KR20130011778A - The ducational microorganism culture kit using carbon dioxide - Google Patents

The ducational microorganism culture kit using carbon dioxide Download PDF

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KR20130011778A
KR20130011778A KR1020110073179A KR20110073179A KR20130011778A KR 20130011778 A KR20130011778 A KR 20130011778A KR 1020110073179 A KR1020110073179 A KR 1020110073179A KR 20110073179 A KR20110073179 A KR 20110073179A KR 20130011778 A KR20130011778 A KR 20130011778A
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water
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박세훈
이미애
김창수
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박세훈
이미애
김창수
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Abstract

PURPOSE: An educational microorganism culture kit using carbon dioxide is provided to easily proliferate microorganisms and to easily observe the microorganisms with naked eyes. CONSTITUTION: An educational microorganism culture kit comprises: a water heating device(103) which heats water contained in a culture tank(50) at a predetermined temperature; a temperature control device(105) mounted at the upper end of the other side; a protection cover(108) for proliferation and maintenance, mounted at the upper portion of the culture tank; an air supply tube(203) around the lower side of the inner wall of the culture tank; an auxiliary air supply device(208) at one side of the air supply tube; and a filtering tank(304) of a predetermined volume for filtering the microorganisms.

Description

이산화탄소를 이용한 교육용 미생물 배양 키트{The ducational microorganism culture kit using carbon dioxide}The ducational microorganism culture kit using carbon dioxide

본 발명은 이산화탄소를 이용한 교육용 미생물 배양 키트에 관한 것으로, 오래전부터 지구상에 존재하는 미생물(식물성 플랑크톤)을 교육용으로 쉽게 간편하게 배양/증식할 수 있는 배양키트에 관한 것이다.
The present invention relates to an educational microbial culture kit using carbon dioxide, and relates to a culture kit that can easily culture / grow the microorganisms (phytoplankton) existing on Earth for a long time for education.

일반적으로 미생물(식물성 플랑크톤)은 지구상에서 오래전부터 존재해왔으며 미생물에 필요한 여러 가지 환경조건에 따라 다양한 형태로 존재하고 그 수 또한 엄청난 양이 우리 눈엔 보이지 않지만 많은 개체가 존재하고 있다.
In general, microorganisms (phytoplankton) have existed for a long time on the earth and exist in various forms according to the various environmental conditions required for microorganisms, and the number is also invisible to our eyes, but there are many individuals.

이러한, 모든 생물의 기본 구성요소인 물, 햇빛을 이용하여 광합성 성장이 가능한 단세포성미생물(식물성 플랑크톤)중 이산화탄소를 주 에너지원으로 이용하여 물, 과 적절한 햇빛이 공급되면 서로 유기적인 반응을 통해 미생물 번식이 이루어 지게 되는데,
In the single-celled microorganism (phytoplankton) capable of photosynthetic growth using water and sunlight, which are the basic components of all living organisms, carbon dioxide is used as the main energy source, and when water and proper sunlight are supplied, microorganisms are organically reacted with each other. Breeding takes place,

이러한 미생물들은 눈에 보이지 않는 생물체이지만 최근 들어 인류사회가 이러한 미생물을 이용하여 인간에게 필요한 대체 에너지자원으로 점차 각광을 받고 있어, 이를 이용한 미생물을 다양한 형태의 에너지를 대체할 수 있는 여러 가지 연구개발이 진행되고 있다.These microorganisms are invisible organisms, but in recent years, the human society has been increasingly spotlighted as an alternative energy source for humans using these microorganisms. It's going on.

또한, 이러한 개발이 최근 들어 증가하는 요인으로 지구온난화가 중요 요인으로 작용하여 이산화탄소가 이러한 지구온난화의 한요소로 작용되고 있고, 이러한 요인에 의해 막대한 피해가 발생되고, 이런 문제점을 해결하기 위한 수단의 한가지로 이산화탄소를 주 에너지원으로 하는 미생물을 배양시켜 공기 중에 있는 이산화탄소를 에너지 원화 시키고 대기중의 이산화탄소를 억제할 수 있는 점에서 최근 들어 여러 가지 방안으로 연구되고 있으나, 이러한 미생물을 이용하여 열대우림보다 단위 면적당 2~3배 높은 이산화탄소 흡수력을 보이는 수만 종의 미생물을 이용하여 미래식량, 기능성 건강식품, 화장품 원료, 방사능 오염물질 제거등 효용가치가 높은 미래생명자원으로 새로운 분야로 주목받고 있는 교육적인 면에서도 많은 활용가치가 있어 최근 들어 주목받고 있는 실정이다.In addition, global warming is an important factor in the recent increase of such development, and carbon dioxide is acting as a factor of global warming, and huge damage is caused by these factors, and means of solving the problems For example, in recent years, various methods have been studied to cultivate microorganisms using carbon dioxide as a main energy source to make energy in the air and to suppress carbon dioxide in the air. The educational side that is attracting attention as a new field as a future life resource with high utility value such as future food, functional health food, cosmetic raw materials, and radioactive contaminant removal using tens of thousands of microorganisms showing 2-3 times higher CO2 absorption capacity per unit area. There are many useful values in For example, the situation is attracting attention.

그러나 이러한 미생물에 대해 단순히 미생물의 의미 정도만 이해할 뿐 다양한 형태의 미생물을 일반인 혹은 학생들에게 교육적인 정보 제공에는 한계가 있어 단순한 이론적인 암기 위주의 교육에만 치중하게 되어 실질적인 실습이라던지 미생물이 어떻게 배양/증식되는지에 대해 실질적으로 이용하기가 쉽지 않아 이에 따른 개선안이 요구되고 있는 실정에 있다.
However, they only understand the meaning of microorganisms, and there are limitations in providing educational information to the general public or students of various types of microorganisms. Therefore, they focus only on simple theoretical memorization-based education. It is not easy to use practically whether or not whether there is a need for improvement accordingly.

이러한 이론적인 교육에서 벗어나 직접 미생물을 배양/증식하여 그 결과에 따른 미생물을 채취하여 단순암기 위주가 아닌 체험이나 미생물 배양/증식과 같은 실습적인 부분에 대한 배양키트가 부족하여 이에 대한 문제점에 대한 개선점이 요구되고 있는 실정에 있다.
Improvement of the problem for this problem by cultivating / growing the microorganisms directly from this theoretical education and collecting the microorganisms according to the result and lacking the culture kit for the practical part such as experience or microbial culture / proliferation This is in demand.

따라서, 미생물 배양에 있어서 쉽고 간편하게 미생물 배양을 할 수 있는 배양조건이 쉽지 않기 때문에 교육적이면서 체계적으로 일정 기간 동안 단계별로 진행할 수 있는 실험키트는 없는 상태여서 일정기간 내에 미생물 배양/증식을 쉽게 하기 위한 장비가 보급 되지 않아 실험용 기자재 측면에서 많은 에로 상황이 있어 이에 대한 문제점이 도출되고 있는 실정에 있다.
Therefore, there is no experimental kit that can be progressed step by step for a certain period of time, because it is not easy to culture microorganisms easily and simply in culture, equipment for easy microbial culture / growth within a certain period of time. There is a lot of erotic situations in terms of experimental equipment because it is not widely distributed, which is causing problems.

이에 본 발명에서는 미생물 배양을 눈으로 쉽게 확인할 수 있고 또한 적은 양의 미생물을 이용하여 일정한 시간 동안 미생물 배양/증식에 필요한 배양키트를 구성하여 쉽고 간단하게 교육용 혹은 일반인 누구나 미생물을 배양/증식 실습이 용이하게 할 수 있는 이산화탄소를 이용한 교육용 미생물 배양 키트를 제공함에 주안점을 두고 그 기술적 과제로서 완성한 것이다.
Therefore, in the present invention, the microbial culture can be easily confirmed by visual observation, and by using a small amount of microorganisms, a culture kit necessary for microbial culture / proliferation for a predetermined time can be easily and simply used for educational or general public to easily incubate / promote the microorganisms. The main objective is to provide an educational microbial culture kit using carbon dioxide that can be made and completed as a technical task.

위와 같이 기술적 과제를 달성하기 위한 본 발명은 일정한 용량의 물(W)을 담수 할 수 배양함(50)에 내부 좌측 상단 위치에 물(W)을 일정온도로 가열할 수 있는 물가열장치(103)와 타측 상단에는 온도측정장치(105)를 설치하고, 상기 배양함(50)의 상부에 증식유지보호커버(108)와, 상기 배양함(50)의 내벽 하측 둘레에는 공기공급관(203)을 설치하며, 상기 공기공급관(203) 일측에는 보조공기공급장치(208)를 연결관(207)으로 연통 구성 설치된 것과; 상기 배양함(50)을 통해 배양된 미생물(W-1)을 포함하는 배양함(50) 내의 물(W)을 공급받은 후 그 현탁액 속의 미생물(W-1)을 걸러 낼 수 있게 일정한 용량의 여과통(304)을 설치한 다음, 그 여과통(304) 상단에 조밀한 여과망(303)을 구성시킨 것과; 상기 배양장치(10)와 여과수단(30) 사이에는 배양장치(10)를 통해 배양이 완료된 물(W)을 여과통(304)으로 공급시키기 위한 배수수단(40)을 구성시켜서 된 것이다.
The present invention for achieving the technical problem as described above is a water heater (103) capable of heating the water (W) at a predetermined temperature in the upper left position inside the incubator (50) to the water (W) of a constant capacity. ) And the other end of the temperature measuring device 105 is installed, the proliferation and maintenance protective cover 108 in the upper portion of the incubator 50, and the air supply pipe 203 around the inner wall lower side of the incubator 50 Installed on one side of the air supply pipe 203, the auxiliary air supply device 208 is configured to communicate with the connection pipe 207; After receiving the water (W) in the culture box (50) containing the cultured microorganism (W-1) through the culture box (50) of a constant volume to filter the microorganisms (W-1) in the suspension Installing a filter barrel 304, and then forming a dense filter net 303 on top of the filter barrel 304; Between the culture device 10 and the filtration means 30 is to configure the drainage means 40 for supplying the water (W) culture is completed through the culture device 10 to the filter barrel (304).

이와 같이 구성된 이산화탄소를 이용한 교육용 미생물 배양 키트는 전술된 바와 같이, Educational microbial culture kit using the carbon dioxide configured as described above,

미생물 배양에 필요한 배양/증식 키트가 갖추어져 있는 상태에서 소량의 배양용 미생물과 촉진제를 투입하여 일정한 시간 동안 온도와 외부 조도가 공급되면 미생물이 쉽게 증식 배양되도록 하여 일정한 기간 경과에 따른 미생물 고유의 색깔을 단계적 시간상으로 확인할 수 있고, 이러한 또한 이러한 미생물을 직접 일정기간 증식을 효과를 통해 쉽게 육안으로 미생물을 직접 손으로 촉각, 육각, 후각 등을 직접 확인할 수 있는 효과가 있어 그 교육적인 측면에서 효과가 있는 등 그 기대하는 바가 실로 다대한 발명이라 할 수 있다.
When a small amount of microorganisms for cultivation and a promoter are added and the temperature and external illuminance are supplied for a certain time while the culture / proliferation kit necessary for microbial culture is in place, the microorganisms are easily multiplied and cultured so that the unique color of the microorganisms can be maintained over a certain period of time. Step by step time, and also through the effect of direct growth of these microorganisms for a certain period of time, it is effective to directly identify the tactile, hexagonal, olfactory, etc. directly with the naked eye by the naked eye, which is effective in the educational aspect. Indeed, it is a great invention.

도 1은 본 발명의 바람직한 실시 상태 예시도
도 2는 본 발명의 배양장치에 대한 평면 확대 예시도
도 3은 본 발명의 또 따른 배수수단을 나타낸 예시도FIG. 1 is a diagram illustrating a preferred embodiment of the present invention
Figure 2 is an enlarged plan view of the culture apparatus of the present invention
3 is an exemplary view showing another drainage means of the present invention

본 발명의 이산화탄소를 이용한 교육용 미생물 배양 키트는 첨부된 각 도면에 의거보다 상세히 설명하면 하기와 같다.
Educational microbial culture kit using carbon dioxide of the present invention will be described in more detail based on the accompanying drawings.

일측에는 미생물 배양을 위한 배양장치(10)가 설치되고, 다른 일측에는 배양된 미생물을 여과하는 여과수단(30)이 설치되는 구성에 있어서,
In one side is provided with a culture apparatus 10 for culturing microorganisms, the other side is provided with a filtration means 30 for filtering the cultured microorganisms,

상기 배양장치(10)는 일정한 용량(10~20리터)의 물(W)을 담수 할 수 배양함(50)을 설치하고,The incubator 10 is installed in a fresh water incubator 50 to a certain capacity (10-20 liters) of water (W),

상기 배양함(50) 내부 좌측 상단 위치에 물(W)을 일정온도로 가열할 수 있는 물가열장치(103)를 설치하며,Install a water heater 103 for heating the water (W) to a predetermined temperature in the upper left position inside the incubator 50,

상기 물가열장치(103)와 타측 상단에는 물(W) 온도를 실시간 측정가능한The water heating device 103 and the other upper end of the water (W) temperature can be measured in real time

온도측정장치(105)를 설치하고,Install the temperature measuring device 105,

상기 배양함(50)의 상부에는 이물질 투입 방지를 위한 증식유지보호커버(108)를 설치하며,In the upper portion of the culture box 50 is installed a proliferation and maintenance protective cover 108 to prevent the entry of foreign substances,

상기 배양함(50)의 내벽 하측 둘레에는 외부로부터 공기펌프(P)로 부터 공기를 공급받아 일정 등간격으로 다수의 공기분사구(203a)가 뚫려 있는 동시에 전체가 인라인으로 연결된 공기공급관(203)을 설치하고,The inner circumference of the inner wall of the incubator 50 receives air from the air pump P from the outside and a plurality of air injection holes 203a are drilled at regular intervals, and at the same time, the air supply pipe 203 is connected inline. Install it,

상기 공기공급관(203) 일측에는 상기 공기공급관(203)과 별도의 위치에서 추가적으로 공기를 분사할 수 있는 보조공기공급장치(208)를 연결관(207)으로 연통 구성 설치된 것과;
One side of the air supply pipe 203 is provided with an auxiliary air supply device 208 which is configured to communicate with the connection pipe 207 to inject additional air at a separate position from the air supply pipe 203;

상기 여과수단(30)은, 배양함(50)을 통해 배양된 미생물(W-1)을 포함하는 배양함(50) 내의 물(W)을 공급받은 후 그 현탁액 속의 미생물(W-1)을 걸러 낼 수 있게 일정한 용량의 여과통(304)을 설치한 다음, 그 여과통(304) 상단에 조밀한 여과망(303)을 구성시킨 것과;
The filtering means 30 receives the water (W) in the culture box 50 including the microorganisms (W-1) cultured through the culture box 50, and then the microorganisms (W-1) in the suspension. Installing a filter vessel 304 of a constant capacity so as to filter out, and then forming a dense filter network 303 on top of the filter vessel 304;

상기 배양장치(10)와 여과수단(30) 사이에는 배양장치(10)를 통해 배양이 완료된 물(W)을 여과통(304)으로 공급시키기 위한 배수수단(40)을 더 설치한 것; 을 특징으로 한다.
Between the culture device 10 and the filtration means 30 is further provided with drainage means 40 for supplying the water (W) culture is completed through the culture device 10 to the filter barrel (304); .

상기 배수수단(40)은 배양함(50) 내부 바닥면에 호스(308) 일측 끝단을 삽입 후, 그 반대측 끝단은 배양장치(10) 보단 낮은 곳에 여과수단(30) 설치하여 작업자가 흡입하면 여과수단(30)으로 물(W)이 배출되도록 설치한 것을 또 다른 특징으로 한다.
The drainage means 40 is inserted into one end of the hose 308 on the bottom surface of the incubator 50, the opposite end is installed in the lower filter than the incubator 10, the filter means 30 is installed and filtered by the operator Another feature is that the water (30) is installed so as to discharge the water (W) to the means (30).

또한, 상기 배수수단(40)은 배양함(50) 내부 아래쪽에 좁아지는 배수로(401)를 구성하되, 그 배수로(401) 전, 후측은 경사면(403)이 구성되도록 설치하며, In addition, the drainage means 40 constitutes a drainage passage 401 that is narrowed below the inside of the incubator 50, and before and after the drainage passage 401 is installed so that the inclined surface 403 is configured,

상기 배수로(401)의 하측에는 조절식레버(402)가 구성되어, 그 하측에 여과수단(30)을 설치되는 것을 또 다른 특징으로 한다.
The control lever 402 is configured at the lower side of the drainage passage 401, and is characterized in that the filtering means 30 is installed at the lower side thereof.

위와 같이 구성될 수 있는 본 발명의 이산화탄소를 이용한 교육용 미생물 배양 키트는 전술된 바와 같이,
Educational microbial culture kit using the carbon dioxide of the present invention can be configured as described above,

미생물 배양을 위해서는 일정한 용량의 10~20리터의 물(W)을 담수 할 수 있는 크기의 유리 재질의 혹은 강화 플라스틱 수지 재로 구성되며 그 형상은 판형 혹은 원형상으로 구성되는 배양함(50)에 미생물 배양 종자를 투입 후 원활한 배양 증식을 위해 구획부 구성된 배양함(50) 내부 물(W)의 온도를 36℃~38℃로 일정하게 유지시켜 주는 물가열장치(103)가 물(W)이 채워진 상태에서 물(W) 내부에 설치되어 미생물 배양이 가장 적절하게 배양/증식 일정한 온도를 유지하도록 하고,For microbial cultivation, it consists of glass or reinforced plastic resin material of the size that can hold 10 ~ 20 liters of water (W) of constant capacity, and its shape is microorganisms The water heating device 103 which maintains the temperature of the water (W) in the compartment 50 for a smooth growth of the culture after the seed is added at 36 ° C. to 38 ° C. is filled with water (W). It is installed inside the water (W) in the state so that the microbial culture is most appropriately maintained at a constant temperature for incubation / proliferation,

상기 배양/증식 작용중에 외부로부터 기타 이물질 및 먼지가 들어가지 않도록 배양함(50) 상부에 증식유지보호커버(108)가 구성되어 상측에 덮개 되어 외부로부터 이물질을 방지와 온도를 일정하게 유지할 수 있도록 작동하고,
The growth and proliferation protection cover 108 is configured on the upper side of the culture 50 to prevent other foreign substances and dust from entering during the culture / proliferation operation, and is covered on the upper side to prevent foreign substances from the outside and maintain a constant temperature. Works,

또한, 공기중에 포함된 (이산화탄소+산소)가 섞여 있는 공기를 외부에 별도로 구성된 공기펌프(P)에 의해 배양함(50) 내부 공기공급관(203)과 보조공기공급장치(208)로 공기를 공급시켜 미생물이 필요로 하는 이산화 탄소를 공기공급관(203)를 통해 원활히 배출됨과 동시에 공기공급관(203)의 공기분사구(203a)에 의해 배양함(50) 내부 벽면에 미생물(W-1)이 흡착되지 않도록 작동하고,
In addition, the air containing (carbon dioxide + oxygen) contained in the air is incubated by an air pump (P) configured separately (50) to supply air to the internal air supply pipe 203 and the auxiliary air supply device 208. The carbon dioxide required by the microorganisms is smoothly discharged through the air supply pipe 203 and at the same time incubated by the air injection port 203a of the air supply pipe 203. Work so that

상기 여과수단(30)은, 배양함(50)을 통해 배양된 일정기간 동아 배양/증식된 미생물(W-1)을 포함하는 물(W)은 그 현탁액 속의 미생물(W-1)을 걸러 낼 수 있게 일정한 용량의 여과통(304)을 설치한 다음, 그 여과통(304) 상단에 조밀한 여과망(303)을 구성시켜 작동 되도록 하고,
The filtration means 30, the water (W) containing the microorganism (W-1) cultured / proliferated for a certain period of time cultivated through the culture box (50) to filter out the microorganism (W-1) in the suspension Install a constant capacity filter barrel 304, and then configure a dense filter net 303 on top of the filter barrel 304 to operate,

상기 배양장치(10)와 여과수단(30) 사이에는 배양장치(10)를 통해 배양/증식이 완료된 물(W)을 여과통(304)으로 공급시키기 위한 배수수단(40)을 더 설치하여 다양한 방법으로 여과를 할 수 있도록 하고,
Between the incubator 10 and the filtering means 30 by installing a drainage means 40 for supplying the water (W) cultivation / growth is completed through the incubator 10 to the filter barrel 304, various methods To allow filtration

상기 배수수단(40)은 배양함(50) 내부 바닥면에 호스(308) 일측 끝단을 삽입후, 그 반대측 끝단은 배양장치(10) 보단 낮은 곳에 여과수단(30) 설치하여 작업자가 일정시간 동안 빨아 댕기게 되면 배양장치(10)와 여과수단(30)과의 높낮이 단차로 인해 중력에 의해 아래쪽으로 이동되도록 한 원리를 이용하여 쉽고 간편하게 여과수단(30)으로 배출되도록 작동할 수도 있고,
The drain means 40 is inserted into one end of the hose 308 on the bottom surface of the incubator 50, the opposite end is installed in the filter means 30 in a lower position than the culture apparatus 10 for the operator for a predetermined time When sucked out, it may operate to be easily and conveniently discharged to the filtration means 30 by using the principle of being moved downward by gravity due to the height difference between the culture apparatus 10 and the filtration means 30,

추가적으로, 상기 배수수단(40)은 배양함(50) 내부 아래쪽에 좁아지는 배수로(401)를 구성하여 그 아래쪽에 구성된 경사면(403)에 의해 한쪽으로 물(W)이 모이면서 배출될 수 있는 구조적인 특징이 있고, 그 아래쪽에 구비된 조절식레버(402)에 의해 여과수단(30)으로 배출되도록 작동할 수 있는 것을 또 다른 특징으로 한다.
In addition, the drainage means 40 has a structure that can be discharged while collecting the water (W) to one side by the inclined surface 403 configured to form a drainage passage 401 narrower in the lower side of the incubator 50 And it is another feature that can be operated to be discharged to the filtration means 30 by the adjustable lever 402 provided below it.

W: 물 W-1: 미생물
10: 배양장치 30: 여과수단 40: 배수수단 50: 배양함
103: 물가열장치 105: 온도측정장치
108: 증식유지보호커버 203: 공급파이프
203a: 공기분사구 207: 연결관
208: 보조공기공급장치 303: 여과망
304: 여과통 401: 배수로
402: 조절식레버 403: 경사면W: water W-1: microorganism
10: culture device 30: filtration means 40: drainage means 50: culture vessel
103: water heating device 105: temperature measuring device
108: growth holding protective cover 203: supply pipe
203a: air nozzle 207 connector
208: auxiliary air supply device 303: filtering network
304: Filter 401: Drainage
402: adjustable lever 403: inclined surface

Claims (3)

일측에는 미생물 배양을 위한 배양장치(10)가 설치되고, 다른 일측에는 배양된 미생물을 여과하는 여과수단(30)이 설치되는 구성에 있어서,

상기 배양장치(10)는 일정한 용량(10~20리터)의 물(W)을 담수 할 수 배양함(50)을 설치하고,
상기 배양함(50) 내부 좌측 상단 위치에 물(W)을 일정온도로 가열할 수 있는 물가열장치(103)를 설치하며,
상기 물가열장치(103)와 타측 상단에는 물(W) 온도를 실시간 측정가능한
온도측정장치(105)를 설치하고,
상기 배양함(50)의 상부에는 이물질 투입 방지를 위한 증식유지보호커버(108)를 설치하며,
상기 배양함(50)의 내벽 하측 둘레에는 외부로부터 공기펌프(P)로 부터 공기를 공급받아 일정 등간격으로 다수의 공기분사구(203a)가 뚫려 있는 동시에 전체가 인라인으로 연결된 공기공급관(203)을 설치하고,
상기 공기공급관(203) 일측에는 상기 공기공급관(203)과 별도의 위치에서 추가적으로 공기를 분사할 수 있는 보조공기공급장치(208)를 연결관(207)으로 연통 구성 설치된 것과;

상기 여과수단(30)은, 배양함(50)을 통해 배양된 미생물(W-1)을 포함하는 배양함(50) 내의 물(W)을 공급받은 후 그 현탁액 속의 미생물(W-1)을 걸러 낼 수 있게 일정한 용량의 여과통(304)을 설치한 다음, 그 여과통(304) 상단에 조밀한 여과망(303)을 구성시킨 것과;

상기 배양장치(10)와 여과수단(30) 사이에는 배양장치(10)를 통해 배양이 완료된 물(W)을 여과통(304)으로 공급시키기 위한 배수수단(40)을 더 설치한 것; 을 특징으로 포함하는 이산화탄소를 이용한 교육용 미생물 배양 키트.
In one side is provided with a culture apparatus 10 for culturing microorganisms, the other side is provided with a filtration means 30 for filtering the cultured microorganisms,

The incubator 10 is installed in a fresh water incubator 50 to a certain capacity (10-20 liters) of water (W),
Install a water heater 103 for heating the water (W) to a predetermined temperature in the upper left position inside the incubator 50,
The water heating device 103 and the other upper end of the water (W) temperature can be measured in real time
Install the temperature measuring device 105,
In the upper portion of the culture box 50 is installed a proliferation and maintenance protective cover 108 to prevent the entry of foreign substances,
The inner circumference of the inner wall of the incubator 50 receives air from the air pump P from the outside and a plurality of air injection holes 203a are drilled at regular intervals, and at the same time, the air supply pipe 203 is connected inline. Install it,
One side of the air supply pipe 203 is provided with an auxiliary air supply device 208 which is configured to communicate with the connection pipe 207 to inject additional air at a separate position from the air supply pipe 203;

The filtering means 30 receives the water (W) in the culture box 50 including the microorganisms (W-1) cultured through the culture box 50, and then the microorganisms (W-1) in the suspension. Installing a filter vessel 304 of a constant capacity so as to filter out, and then forming a dense filter network 303 on top of the filter vessel 304;

Between the culture device 10 and the filtration means 30 is further provided with drainage means 40 for supplying the water (W) culture is completed through the culture device 10 to the filter barrel (304); Educational microbial culture kit using carbon dioxide comprising a.
제 1항에 있어서,
상기 배수수단(40)은 배양함(50) 내부 바닥면에 호스(308) 일측 끝단을 삽입 후, 그 반대측 끝단은 배양장치(10) 보단 낮은 곳에 여과수단(30) 설치하여 작업자가 흡입하면 여과수단(30)으로 물(W)이 배출되도록 설치한 것; 을 특징으로 포함하는 이산화탄소를 이용한 교육용 미생물 배양 키트.
The method of claim 1,
The drainage means 40 is inserted into one end of the hose 308 on the bottom surface of the incubator 50, the opposite end is installed in the lower filter than the incubator 10, the filter means 30 is installed and filtered by the operator Installed to discharge water (W) to the means (30); Educational microbial culture kit using carbon dioxide comprising a.
제 1항에 있어서,
상기 배수수단(40)은 배양함(50) 내부 아래쪽에 좁아지는 배수로(401)를 구성하되, 그 배수로(401) 전, 후측은 경사면(403)이 구성되도록 설치하며,
상기 배수로(401)의 하측에는 조절식레버(402)가 구성되어, 그 하측에 여과수단(30)을 설치되는 것; 을 특징으로 포함하는 이산화탄소를 이용한 교육용 미생물 배양 키트.


The method of claim 1,
The drainage means 40 constitutes a drainage passage 401 that is narrowed below the inside of the incubator 50, and is installed to form an inclined surface 403 before and after the drainage passage 401.
An adjustable lever 402 is formed at the lower side of the drainage path 401, and a filtering means 30 is installed at the lower side thereof; Educational microbial culture kit using carbon dioxide comprising a.


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KR101967772B1 (en) 2018-09-21 2019-04-10 오성환 Ventilation device
KR102163367B1 (en) 2019-12-05 2020-10-08 오성환 Ventilation device
KR102197205B1 (en) 2020-05-07 2020-12-31 오세헌 Ventilation device
KR20210147943A (en) 2020-05-28 2021-12-07 연세대학교 산학협력단 Apparatus and Method for Interaction with Microorganisms
CN113462550A (en) * 2021-07-01 2021-10-01 常州市金坛第一人民医院 Special constant temperature preservation system of microbial cultivation for medical treatment
KR102633617B1 (en) 2023-05-04 2024-02-05 오세헌 Ventilation device
KR102633618B1 (en) 2023-07-28 2024-02-05 주식회사 한성넥스 Ventilator

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