CN207733389U - A kind of device of laboratory water planting identification rice root form - Google Patents

A kind of device of laboratory water planting identification rice root form Download PDF

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Publication number
CN207733389U
CN207733389U CN201721851997.7U CN201721851997U CN207733389U CN 207733389 U CN207733389 U CN 207733389U CN 201721851997 U CN201721851997 U CN 201721851997U CN 207733389 U CN207733389 U CN 207733389U
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CN
China
Prior art keywords
centrifuge tube
water planting
culture
root form
rice root
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201721851997.7U
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Chinese (zh)
Inventor
柯学
殷富有
肖素勤
程在全
张敦宇
王玲仙
陈越
付坚
钟巧芳
陈玲
王波
蒋聪
曾民
蒋春苗
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biotechnology and Germplasm Resource Institute of Yunnan Academy of Agricultural Sciences
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Biotechnology and Germplasm Resource Institute of Yunnan Academy of Agricultural Sciences
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Filing date
Publication date
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Priority to CN201721851997.7U priority Critical patent/CN207733389U/en
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Publication of CN207733389U publication Critical patent/CN207733389U/en
Expired - Fee Related legal-status Critical Current
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/20Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
    • Y02P60/21Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures

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  • Cultivation Receptacles Or Flower-Pots, Or Pots For Seedlings (AREA)

Abstract

The utility model discloses a kind of device of laboratory water planting identification rice root form, including floating culture apparatus, centrifuge tube and immersion device, centrifuge tube is fixed in the mounting hole of floating culture apparatus, and the lower ending opening of centrifuge tube and submerge immersion device in, it is put into rice paddy seed in centrifuge tube, culture solution is filled in the device that soaks.The device of laboratory water planting identification rice root form in the utility model, utilize most common material in laboratory, plastic crate, mesh bag, cystosepiment, centrifuge tube are fabricated to water planting plate, cost of manufacture is cheap, it is used convenient for experiment, and it is easy to operate, it is grown to 2 months from rice paddy seed is seeded into, midway need to only carry out the replacement of 1 subculture, and retain original fertile soil when replacing, basic water planting culture solution is only replaced, midway only adds tap water to keep entire culture medium volume, sowing and culture efficiency high in due course;And cultivate rice quantity number, only need on foam culture plate directly according to demand quantity place.

Description

A kind of device of laboratory water planting identification rice root form
Technical field
The utility model is related to water culture experiment engineering device technique fields, and rice root is identified more particularly to a kind of laboratory water planting It is the device of form.
Background technology
For Root morphology qualification test, rice culture can have two major classes:Solid dielectric and water planting.Solid dielectric generally to A certain amount of agar powder, agarose, plant gel are added in culture solution, makes solid medium, sterile culture can be done in pipe. Culture solution can be MS culture mediums, can also be Hydroponic culture.But this method operation is stringent, cumbersome, needs to carry out institute in a organized way Used sterile working in culture, when in face of large sample material, workload is extremely big.Also, since culture volume has Limit, with the lengthening of incubation time, Toxic Metabolites accumulate in the medium, affect the growth of rice, if carrying out solid Culture medium is replaced, and process is more cumbersome, and old culture medium detaches also inconvenience from root.
Therefore, ciltivating process is most common, at present water-culturing rice be all based on International Rice culture medium, or herein On the basis of the culture medium changed.For cultivating utensil, (tubule of bottom is cut off a duck eye) of useful PCR plate, but PCR plate Aperture is small, limit paddy growth, be placed in culture solution as time goes by, rice grow up weight increasing, can sink.Therefore, this Kind method is only applicable to Seedling Stage.
The problem encountered in water planting is to be easy microbiological contamination, and due to there was only rice root in water body, being harmful to root fungus also can be attached It on root.If solving the problems, such as this, water body is only frequently replaced, generally at least needs to change within 1 week a water.Due to rice Root system is longer (tens centimetres) when water planting, in order to not influence root growth form, it is ensured that the enough depth of water.Therefore, flood is replaced Body becomes time-consuming and laborious and expense culture medium.Moreover it is found by our experiment, it, will not be because of more for lived mushroom It changes water body and is just diffused into other regions, still concentrate on root, therefore, for long-time culture, replacing water body can not Solve the problems, such as that microbiological contamination finally results in root rot well.
Utility model content
The purpose of this utility model is to provide a kind of device of laboratory water planting identification rice root form, above-mentioned to solve Problem of the existing technology, the hydroponic device is efficient, convenient, simple, of low cost, and root system can be kept to give birth to greatest extent Long form.
To achieve the above object, the utility model provides following scheme:The utility model provides a kind of laboratory water planting Identify that the device of rice root form, including floating culture apparatus, centrifuge tube and immersion device, the centrifuge tube are fixed on described In the mounting hole for floating culture apparatus, and the lower ending opening of the centrifuge tube and submerge in the immersion device, the centrifuge tube In be put into rice paddy seed, fill culture solution in the immersion device.
Optionally, the floating culture apparatus is foam culture plate.
Optionally, the diameter of the mounting hole on the foam culture plate is identical as the outer diameter of the centrifuge tube.
Optionally, the diameter of the mounting hole on the foam culture plate is more than the outer diameter of the centrifuge tube.
Optionally, the outer sheath of the foam culture plate be equipped with a mesh bag, the centrifuge tube sequentially pass through upper layer mesh bag and Foam culture plate simultaneously immerses in the immersion device after being contacted with lower layer mesh bag.
Optionally, the quantity for the rice paddy seed being put into each centrifuge tube is 1-3.
Optionally, the immersion device is to fill with the plastic crate of water, and the open area of the plastic crate is more than or equal to institute State the cross-sectional area of floating culture apparatus.
Optionally, the bottom end center of the immersion device offers an osculum being recessed inwardly, at the osculum It is tightly connected drain cap, and the bottom surface for installing the immersion device of the drain cap is plane.
The utility model achieves following technique effect compared with the existing technology:
The device of laboratory water planting identification rice root form in the utility model, utilizes most common material in laboratory Material, plastic crate, mesh bag, cystosepiment, centrifuge tube are fabricated to water planting plate, and cost of manufacture is cheap, are used convenient for experiment, and operation side Just, grow to 2 months from being seeded into rice paddy seed, midway need to only carry out the replacement of 1 subculture, and while replacing retain it is original Fertile soil, only replaces basic water planting culture solution, and midway only adds tap water to keep entire culture medium volume, sowing and culture in due course Ultrahigh in efficiency;And cultivate rice quantity number, only need on foam culture plate directly according to demand quantity place.
Description of the drawings
In order to illustrate the embodiment of the utility model or the technical proposal in the existing technology more clearly, below will be to embodiment Needed in attached drawing be briefly described, it should be apparent that, the accompanying drawings in the following description is only the utility model Some embodiments for those of ordinary skill in the art without having to pay creative labor, can also basis These attached drawings obtain other attached drawings.
Fig. 1 is the overall structure diagram for the device that the utility model laboratory water planting identifies rice root form;
Wherein, 1 rice;2 foam culture plates;3 centrifuge tubes;4 plastic crates;5 mesh bags.
Specific implementation mode
The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are without making creative work The every other embodiment obtained, shall fall within the protection scope of the present invention.
The purpose of this utility model is to provide a kind of device of laboratory water planting identification rice root form, above-mentioned to solve Problem of the existing technology, the hydroponic device is efficient, convenient, simple, of low cost, and root system can be kept to give birth to greatest extent Long form.
The device of laboratory water planting provided by the utility model identification rice root form, including floating culture apparatus, from Heart pipe and immersion device, centrifuge tube is fixed in the mounting hole of floating culture apparatus, and the lower ending opening of centrifuge tube and submerges leaching In water installations, it is put into rice paddy seed in centrifuge tube, culture solution is filled in the device that soaks.
Using most common material in laboratory, plastic crate, mesh bag, cystosepiment, centrifuge tube are fabricated to water planting plate, are fabricated to This is cheap, is used convenient for experiment, and easy to operate, rice paddy seed is put into centrifuge tube and fills the modeling of culture solution by being immersed in Nutrition needed for growth is provided for rice paddy seed, and floating culture apparatus and centrifuge tube are picked up and can directly observe rice root in charging basket The form and upgrowth situation of system.
To keep the above objects, features, and advantages of the utility model more obvious and easy to understand, below in conjunction with the accompanying drawings and have Body embodiment is described in further detail the utility model.
It please refers to Fig.1, wherein Fig. 1 is the entirety for the device that the utility model laboratory water planting identifies rice root form Structural schematic diagram.
As shown in Figure 1, the utility model provides a kind of device of laboratory water planting identification rice root form, including floating Culture apparatus, centrifuge tube 3 and immersion device, centrifuge tube 3 are fixed in the mounting hole of floating culture apparatus, and under centrifuge tube 3 End opening simultaneously submerges in immersion device, and the seed of rice 1 is put into centrifuge tube 3, culture solution is filled in the device that soaks.
Floating culture apparatus is foam culture plate 2, the diameter of mounting hole and being combined with for centrifuge tube 3 on foam culture plate 2 Two kinds, it is identical as the outer diameter of centrifuge tube 3 that one is the diameters of the mounting hole on foam culture plate 2;Another kind is foam culture plate 2 On mounting hole diameter be more than centrifuge tube 3 outer diameter.When the diameter of the mounting hole on foam culture plate 2 is more than centrifuge tube 3 When outer diameter, the outer sheath of foam culture plate 2 is equipped with a mesh bag 5, and centrifuge tube 3 sequentially passes through upper layer mesh bag and foam culture plate 2 simultaneously It is immersed after being contacted with lower layer mesh bag in immersion device.
The quantity for the rice paddy seed being put into each centrifuge tube 3 is 1-3;Immersion device be fill with the plastic crate 4 of water, and The open area of plastic crate 4 is more than or equal to the cross-sectional area of floating culture apparatus.
The bottom end center of immersion device offers an osculum being recessed inwardly, and drain cap is tightly connected at osculum, And the bottom surface for installing the immersion device of drain cap is plane, during cultivating rice paddy seed, integrally can directly be put device It sets on platform or on shelf, when needing replacing culture solution halfway, without that will soak device and foam culture plate 2 detaches, Drain cap is directly opened, the culture solution in immersion device is discharged by osculum, then seals drain cap, then by immersion device Top is directly injected into new culture solution.
In this experimental provision:
The fertile soil of 1/4 volume ratio is added in Hydroponic culture, culture medium is made suspension.On the one hand rich in this way Rich nutrition, in addition more importantly numerous humic soil particle provides attachment and living space for thalline.Formation one is several The root growth ecosystem of nearly soil.Suitable fertile soil is added, the morphologic observation of last root will not be influenced, as long as using water It gently rinses, has just obtained complete root system.
We are in such a way that floating is cultivated, in flower plants and nursery stock market, buy very often and very it is cheap (3 one piece of yuans, often The foam culture plate 2 in 200 hole of block.) vertical growth for rice 1 on the water, it to consider the convenience issue of germination, also want Consider the support problem of plant strain growth.Since the aperture of cystosepiment bottom in the market is somewhat big for rice paddy seed, I In one mesh bag 5 of cystosepiment housing, will not be leaked downwards when mesh both ensure that the germination of rice 1, and also ensure growth When root lift and the support to entire plant.Pass through the 5ml centrifuge tubes of one base cuts mouth of insertion on cystosepiment wire side again 3.The advantage of doing so is that ensureing that plant passes through wire side, vertical growth non-lodging.Cystosepiment is finally placed directly in one In the plastic crate 4 for filling with water, it is put into 1-3 healthy seeds in each centrifuge tube 3, is placed in phjytotron light culture, temperature 32 DEG C, humidity can not have to adjustment, to reach optimal effect, can be adjusted to 90% yet.It sprouts 5-7 days.Cotyledon stretches out centrifugation Outside pipe 3, illumination condition in prepared suspended culture medium can be changed to and grown.Or it is directly given birth on the bright and clear window side in laboratory It is long.Experiment in the utility model is grown to 2 months from 1 material of rice is seeded into, and midway only carried out the replacement of 1 subculture, And retain original fertile soil when replacing.Basic water planting culture solution is only replaced, midway only adds tap water to keep entire training in due course Support medium volume.Using the experimental provision, a people can complete the sowing and culture of nearly 1000 parts of materials, ultrahigh in efficiency for one day. The thinking of this experiment be suitble to sample number, as long as doing correspondingly sized adjustment on culture utensil.
It should be noted that the device of the laboratory water planting identification rice root form in the utility model, floating training Foam culture plate and plastic crate might not be limited only to by supporting the selection of device and the device that soaks, as long as can meet corresponding use The other materials of demand also can, it is within the protection scope of the present utility model;In addition, the selection of centrifuge tube and mesh bag also not office Limit, material or the test tube that can meet corresponding requirement are also within the protection scope of the present utility model;The utility model Data in embodiment content are also only the explanation and implementation for playing the role of one to scheme, and transformation uses the foam of other specifications Plate, centrifuge tube or plastic crate etc. are also within the protection scope of the present utility model.
Specific case is applied in the utility model to be expounded the principles of the present invention and embodiment, it is above The explanation of embodiment is merely used to help understand the method and its core concept of the utility model;Meanwhile for the one of this field As technical staff, according to the utility model thought, there will be changes in the specific implementation manner and application range.To sum up Described, the content of the present specification should not be construed as a limitation of the present invention.

Claims (8)

1. a kind of device of laboratory water planting identification rice root form, it is characterised in that:Including floating culture apparatus, centrifuge tube With immersion device, the centrifuge tube is fixed in the mounting hole of the floating culture apparatus, and the lower ending opening of the centrifuge tube And submerge in the immersion device, it is put into rice paddy seed in the centrifuge tube, culture solution is filled in the immersion device.
2. the device of laboratory water planting identification rice root form according to claim 1, it is characterised in that:The floating Culture apparatus is foam culture plate.
3. the device of laboratory water planting identification rice root form according to claim 2, it is characterised in that:The foam The diameter of mounting hole on culture plate is identical as the outer diameter of the centrifuge tube.
4. the device of laboratory water planting identification rice root form according to claim 2, it is characterised in that:The foam The diameter of mounting hole on culture plate is more than the outer diameter of the centrifuge tube.
5. the device of laboratory water planting identification rice root form according to claim 4, it is characterised in that:The foam The outer sheath of culture plate is equipped with a mesh bag, and the centrifuge tube sequentially passes through upper layer mesh bag and foam culture plate and connect with lower layer mesh bag It immerses after touch in the immersion device.
6. the device of laboratory water planting identification rice root form according to claim 1, it is characterised in that:It is each described The quantity for the rice paddy seed being put into centrifuge tube is 1-3.
7. the device of laboratory water planting identification rice root form according to claim 1, it is characterised in that:The immersion Device is to fill with the plastic crate of water, and the open area of the plastic crate is more than or equal to the cross section of the floating culture apparatus Product.
8. the device of laboratory water planting identification rice root form according to claim 1, it is characterised in that:The immersion The bottom end center of device offers an osculum being recessed inwardly, is tightly connected drain cap at the osculum, and install The bottom surface of the immersion device of the drain cap is plane.
CN201721851997.7U 2017-12-26 2017-12-26 A kind of device of laboratory water planting identification rice root form Expired - Fee Related CN207733389U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201721851997.7U CN207733389U (en) 2017-12-26 2017-12-26 A kind of device of laboratory water planting identification rice root form

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201721851997.7U CN207733389U (en) 2017-12-26 2017-12-26 A kind of device of laboratory water planting identification rice root form

Publications (1)

Publication Number Publication Date
CN207733389U true CN207733389U (en) 2018-08-17

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112602583A (en) * 2020-11-27 2021-04-06 广东省科学院生物工程研究所 Maize seedling water planting test device

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112602583A (en) * 2020-11-27 2021-04-06 广东省科学院生物工程研究所 Maize seedling water planting test device

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CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20180817

Termination date: 20181226

CF01 Termination of patent right due to non-payment of annual fee