CN207435459U - A kind of incubator of the automatically replaceable culture solution of medical experiment - Google Patents

A kind of incubator of the automatically replaceable culture solution of medical experiment Download PDF

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Publication number
CN207435459U
CN207435459U CN201721368660.0U CN201721368660U CN207435459U CN 207435459 U CN207435459 U CN 207435459U CN 201721368660 U CN201721368660 U CN 201721368660U CN 207435459 U CN207435459 U CN 207435459U
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China
Prior art keywords
liquid
culture solution
incubator
bearing
head
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Expired - Fee Related
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CN201721368660.0U
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Chinese (zh)
Inventor
侯阿娜
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Shengjing Hospital of China Medical University
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Shengjing Hospital of China Medical University
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Priority to CN201721368660.0U priority Critical patent/CN207435459U/en
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Abstract

The utility model provides a kind of incubator of the automatically replaceable culture solution of medical experiment, including fluid reservoir, culturing room, lifting gear, liquid changing device, bogey and PLC controller;The lifting gear includes column, fixed plate, lifting motor, guide post, slide, the block that is spirally connected, screw rod and bearing;The liquid changing device includes drain pipe, drainage head, positive displacement pump, liquid-feeding tube, liquid feeding head, charging valve, electronic flowmeter and drip plate;The bogey is arranged on the bottom of liquid changing device;The bogey includes driving motor, train of reduction gears, conical gear group, pedestal, bearing, support plate, culture dish, locating piece and locating slot;The setting that the utility model passes through lifting gear, liquid changing device and bogey, realize the automatic replacement of cell cultivation process culture solution, it avoids manually replacement culture solution and the problem of easily causing mistake is held inaccurately and changed in liquid to the time, it significantly reduces and changes the liquid time, reduce and change liquid difficulty.

Description

A kind of incubator of the automatically replaceable culture solution of medical experiment
Technical field
The utility model belongs to the automatically replaceable training of medical experiment equipment technical field more particularly to a kind of medical experiment The incubator of nutrient solution.
Background technology
With the continuous development of modern science and technology level, the everyway applied to medicine realizes intelligence, however at present Common culture solution addition and substitute mode are still using the method for manually changing liquid in medical experiment so that experimental implementation seems It is relatively complicated, and easily cause to change the situations such as liquid mistake, culture solution pollution in the process, this obvious hair with modern science and technology Exhibition does not match.Therefore, seem very urgent for the intelligentized research of cell culture.
The Chinese patent that notification number is CN203200259U discloses a kind of cell based on semi-automatic speed governing and changes liquid dress It puts, including changing liquid component and speed-regulating switch, the liquid component that changes includes culture solution draw-off pump and the waste liquid draw-off pump that switching starts, The import and export of the culture solution draw-off pump is connected to the first conduit and the second conduit, the import and export point of the waste liquid draw-off pump respectively The 3rd conduit and the 4th conduit are not connected to, and the speed-regulating switch is electrically connected by conducting wire and culture solution draw-off pump and waste liquid draw-off pump It connects.The utility model is extracted by culture solution draw-off pump in fresh medium to culture dish, and waste liquid draw-off pump will after the completion of culture Waste liquid is extracted out, so as to instead of the operating mode for manually changing liquid, improve work efficiency, in addition culture solution draw-off pump and waste liquid are taken out The rotating speed of pump can be controlled so as to adjust by speed-regulating switch by taking the flow of pump so that change that liquid operation is simpler, it is smart to change liquid Exactness higher.The utility model can be applied to biological cell culture.
Utility model people has found that a kind of cell liquid changing device based on semi-automatic speed governing realizes substitution and manually changes liquid Purpose, but its culture solution draw-off pump and waste liquid draw-off pump are only capable of being directed to single culture dish and be used, and cause cell Culture efficiency is relatively low, limits use.
Notification number is that the Chinese patent of CN206308374U discloses a kind of fully-automatic intelligent cell culture apparatus, it includes Controller, storage chamber, culture chamber, operating cavity and observation chamber, the culture chamber is interior to include the first adjusting mechanism and culturing rack, The cell culture apparatus further includes an isolation ward, first be arranged between culture chamber and isolation ward controls door and setting Second between external environment and isolation ward controls door, and the first pipetting device, the isolation ward are further included in affiliated culture chamber Inside include the second adjusting mechanism, stretched out for capturing the Tissue Culture Flask brought of the first pipetting device and passing through the second control door The second pipetting device outside to isolation ward by setting an isolation ward again in fully-automatic intelligent cell culture apparatus, is used for The medium that culture chamber is contacted with external environment, directly can thus take out cultured cell carefully to avoid staff Outside born of the same parents' culture apparatus, cell culture environment is not interfered with, improves work efficiency, also improves the training quality of cell.
Utility model people has found that a kind of fully-automatic intelligent cell culture apparatus needs first to move for the replacement of culture solution The compounding practices such as liquid mechanism, the second pipetting device and the second adjusting mechanism could be completed to change liquid, and operation is complex, also, its Still single culture dish can only be directed to use, limit the practicality.
Then, in view of this, improvement is studied for existing structure and missing, providing a kind of medical experiment use can be certainly The dynamic incubator for replacing culture solution, to achieve the purpose that with more more practical value.
Utility model content
In order to solve the above-mentioned technical problem, the utility model provides a kind of training of the automatically replaceable culture solution of medical experiment Support case, with solve it is existing manually change cumbersome liquid and easy error and existing cell culture apparatus operation it is complex, carefully The problem of efficiency of born of the same parents' culture is low.
The purpose and effect of the utility model medical experiment incubator of automatically replaceable culture solution, by skill in detail below Art means are reached:
The incubator of the automatically replaceable culture solution of a kind of medical experiment, including babinet, insulating layer, upper cover, partition plate, liquid storage Room, culturing room, heating wire, temperature sensor, waste tank, liquid feeding tank, lifting gear, column, fixed plate, lifting motor are led Column, slide, be spirally connected block, screw rod, bearing, liquid changing device, drain pipe, drainage head, positive displacement pump, liquid-feeding tube, liquid feeding head, charging valve, Electronic flowmeter, drip plate, bogey, driving motor, train of reduction gears, conical gear group, pedestal, bearing, support plate, culture Ware, locating piece, locating slot, chamber door, observation window and PLC controller;The madial wall of the babinet is equipped with insulating layer, the heat preservation Layer is heatproof rock wool material;The inside of the babinet is separated to form two chambers above and below fluid reservoir and culturing room by partition plate;It is described Waste tank and liquid feeding tank are arranged in fluid reservoir;The top of the waste tank and liquid feeding tank is equipped with upper cover in babinet;Institute State the bottom that temperature sensor is installed on partition plate;The outside of the culturing room by hinge on babinet in being hinged with chamber door;It is described Chamber door is equipped with observation window;The PLC controller is arranged on the outer wall of babinet;The lifting gear is arranged in culturing room;It is described Lifting gear includes column, fixed plate, lifting motor, guide post, slide, the block that is spirally connected, screw rod and bearing;The fixed plate fixes peace Top side loaded on column;The lifting motor is installed on the top of fixed plate;The power output end and screw rod of the lifting motor It is connected;The bottom of the fixed plate is equipped with guide post at two in the both sides of screw rod;The slide is sheathed on guide post;It is described to be spirally connected Block is sheathed on screw rod;The block that is spirally connected is an integral structure with slide;The right end of the slide is equipped with the bearing of integral type;Institute Liquid changing device is stated to be installed on bearing;The liquid changing device include drain pipe, drainage head, positive displacement pump, liquid-feeding tube, liquid feeding head, plus Liquid valve, electronic flowmeter and drip plate;The drain pipe and liquid-feeding tube are arranged on bearing;The drain pipe and liquid-feeding tube Bottom is respectively equipped with drainage head and liquid feeding head;The top of the drain pipe and liquid-feeding tube is connected respectively with waste tank and liquid feeding tank It connects;The drain pipe is equipped with positive displacement pump;The liquid-feeding tube is equipped with charging valve and electronic flowmeter;The bogey is arranged on The bottom of liquid changing device;The bogey include driving motor, train of reduction gears, conical gear group, pedestal, bearing, support plate, Culture dish, locating piece and locating slot;The driving motor is connected by train of reduction gears with conical gear group;The pedestal Top is rotatablely connected by bearing and conical gear group;The support plate is fixedly installed on the top of conical gear group;It is described to do Top array locating piece everywhere is distributed with;The bottom of the culture dish is equipped with locating slot;The culture dish is determined by bottom It is in mating connection that position slot is installed on locating piece.
Further, the drainage head and liquid feeding head are semicircular arc structure.
Further, the bottom of the drainage head and liquid feeding head is equipped with drip plate.
Further, the culture dish is right against drainage head in the position on support plate and liquid feeding head is set.
Further, the observation window is right against culture dish setting on horizontal level.
Further, the drain pipe and liquid-feeding tube be located at holder top one section are the hose that helical form is set, position It is plastic rigid pipe in one section of rest base.
Further, the S-type structure of the heating wire is wound on the inner wall of culturing room.
Further, the charging valve is electric-controlled type valve.
Compared with prior art, the utility model has the advantages that:
1. the utility model realizes cell culture by the setting of lifting gear, liquid changing device and bogey The automatic replacement of journey culture solution avoids and manually replaces culture solution and the time is held inaccurate and change mistake is easily caused in liquid Problem significantly reduces and changes the liquid time, reduces and changes liquid difficulty.
2. being equipped with culture dish everywhere on the bogey of the utility model altogether, the training of four groups of cell samples can be achieved at the same time It supports, improves the efficiency of cell culture, strengthen the practicability of the utility model.
3. the culture dish of the utility model is engaged by the groove of bottom with the locating slot on support plate, you can is realized to training The good fixation of ware is supported, can conveniently be taken again when picking and placeing.
Description of the drawings
Fig. 1 is the utility model structure diagram;
Fig. 2 is the utility model external structure schematic diagram;
Fig. 3 is lifting device structure schematic diagram;
Fig. 4 is liquid changing device structure diagram;
Fig. 5 is drainage head structure diagram;
Fig. 6 is bogey structure diagram;
Fig. 7 is carrying board structure schematic diagram;
Fig. 8 is culture dish structure diagram.
In figure:1- babinets, 2- insulating layers, 3- upper covers, 4- partition plates, 5- fluid reservoirs, 6- culturing room, 7- heating wire, 8- temperature Sensor, 9- waste tanks, 10- liquid feeding tanks, 11- lifting gears, 111- columns, 112- fixed plates, 113- lifting motors, 114- Guide post, 115- slides, 116- are spirally connected block, 117- screw rods, 118- bearings, 12- liquid changing devices, 121- drain pipes, 122- drainage heads, 123- positive displacement pumps, 124- liquid-feeding tubes, 125- liquid feeding heads, 126- charging valves, 127- electronic flowmeters, 128- drip plates, 13- carryings Device, 131- driving motors, 132- train of reduction gears, 133- conical gear groups, 134- pedestals, 135- bearings, 136- support plates, 137- culture dishes, 138- locating pieces, 139- locating slots, 14- chamber doors, 15- observation windows, 16-PLC controllers.
Specific embodiment
The utility model is described further below in conjunction with attached drawing:
Embodiment:
As shown in attached drawing 1 to attached drawing 8:
The utility model provides a kind of incubator of the automatically replaceable culture solution of medical experiment, including babinet 1, insulating layer 2, upper cover 3, partition plate 4, fluid reservoir 5, culturing room 6, heating wire 7, temperature sensor 8, waste tank 9, liquid feeding tank 10, lifting gear 11, column 111, fixed plate 112, lifting motor 113, guide post 114, slide 115, be spirally connected block 116, screw rod 117, and bearing 118 changes Liquid device 12, drain pipe 121, drainage head 122, positive displacement pump 123, liquid-feeding tube 124, liquid feeding first 125, charging valve 126, electronic flow Meter 127, drip plate 128, bogey 13, driving motor 131, train of reduction gears 132, conical gear group 133, pedestal 134, axis Hold 135, support plate 136, culture dish 137, locating piece 138, locating slot 139, chamber door 14, observation window 15 and PLC controller 16;It is described The madial wall of babinet 1 is equipped with insulating layer 2, and the insulating layer is heatproof rock wool material;The inside of the babinet 1 is divided by partition plate 4 Every forming fluid reservoir 5 and culturing room two chambers about 6;The waste tank 9 and liquid feeding tank 10 are arranged in fluid reservoir 5;Institute The top for stating waste tank 9 and liquid feeding tank 10 is equipped with upper cover 3 in babinet 1;The temperature sensor 8 is installed on the bottom of partition plate 4 Portion;The outside of the culturing room 6 by hinge on babinet 1 in being hinged with chamber door 14;The chamber door 14 is equipped with observation window 15;Institute PLC controller 16 is stated on the outer wall of babinet 1;The lifting gear 11 is arranged in culturing room 6;The lifting gear 11 includes Column 111, fixed plate 112, lifting motor 113, guide post 114, slide 115, the block 116 that is spirally connected, screw rod 117 and bearing 118;
The fixed plate 112 is fixedly installed in the top side of column 111;The lifting motor 13 is installed on fixed plate 112 Top;The power output end of the lifting motor 13 is connected with screw rod 117;The bottom of the fixed plate 112 is in screw rod 117 Both sides are equipped with guide post 114 at two;The slide 115 is sheathed on guide post 114;The block 116 that is spirally connected is sheathed on screw rod 117; The block that is spirally connected is an integral structure with slide 115;The right end of the slide 115 is equipped with the bearing 118 of integral type;It is described to change liquid Device 12 is installed on bearing 118;The liquid changing device 12 includes drain pipe 121, drainage head 122, positive displacement pump 123, liquid-feeding tube 124th, liquid feeding is first 125, charging valve 126, electronic flowmeter 127 and drip plate 128;The drain pipe 121 and liquid-feeding tube 124 are worn On bearing 118;The bottom of the drain pipe 121 and liquid-feeding tube 124 is respectively equipped with drainage head 122 and liquid feeding first 125;It is described The top of drain pipe 121 and liquid-feeding tube 124 is connected respectively with waste tank 9 and liquid feeding tank 10;The drain pipe 121 is equipped with Positive displacement pump 123;The liquid-feeding tube 124 is equipped with charging valve 126 and electronic flowmeter 127;The bogey 13 is arranged on and changes liquid The bottom of device 12;The bogey 13 includes driving motor 131, train of reduction gears 132, conical gear group 133, pedestal 134th, bearing 135, support plate 136, culture dish 137, locating piece 138 and locating slot 139;The driving motor 131 passes through reducing gear Wheel group 132 is connected with conical gear group 133;The top of the pedestal 134 is rotated by bearing 135 and conical gear group 133 Connection;The support plate 136 is fixedly installed on the top of conical gear group 133;It is described to be distributed with everywhere in the top array for doing 136 Locating piece 138;The bottom of the culture dish 137 is equipped with locating slot 139;The culture dish 137 is pacified by the locating slot 139 of bottom It is in mating connection loaded on locating piece 138.
Wherein, the drainage head 122 and liquid feeding first 125 are semicircular arc structure, and the structure of semicircular arc is in drainage head 122 and liquid feeding it is first 125 disengaging culture dish when, culture solution can freely slide, and avoid being trapped on drainage head 122 and liquid feeding first 125.
Wherein, the bottom of the drainage head 122 and liquid feeding first 125 is equipped with drip plate 128, and drip plate 128 is in liquid feeding Play the role of buffering, can avoid that the cell in culture dish is caused to impact in liquid feeding.
Wherein, the culture dish 137 is right against drainage head 122 in the position on support plate 136 and liquid feeding first 125 is set, training Ware 137 is supported under the rotation of bogey 13, turns to the bottom of drainage head 122 and liquid feeding first 125,122 He of drainage head successively Liquid feeding first 125 does vertical movement up and down under the action of lifting gear 11, so as to fulfill drain and changes liquid.
Wherein, the observation window 15 is right against culture dish 137 on horizontal level and sets, and can be observed through observation window 15 The information such as the state of culture solution in culture dish 137, the color by observing culture solution can substantially judge cultivation stage and culture Whether liquid goes bad.
Wherein, the drain pipe 121 and liquid-feeding tube 124 be located at the top of bearing 118 one section be helical form set it is soft Pipe, one section positioned at 118 bottom of bearing is plastic rigid pipe, and when bearing 118 moves up and down, the hose that helical form is set is stretched Contracting keeps the normal connection of drain pipe 121 and liquid-feeding tube 124 and drainage head 122 and liquid feeding first 125.
Wherein, the 7 S-type structure of heating wire is wound on the inner wall of culturing room 6, and heating wire 7 is uniformly layed in On the inner wall of culturing room 6 so that homogeneous heating, gradient of temperature are steady.
Wherein, the charging valve 126 is electric-controlled type valve, and in liquid feeding, PLC controller 16 controls charging valve 26 to open, Electronic flowmeter 127 monitors flow, and communicates information to PLC controller 16, and when completing liquid feeding, the control of PLC controller 16 adds Liquid valve 126 is closed.
The specifically used mode of the present embodiment and effect:
When the utility model is used, the culture solution and newly added culture solution changed are temporarily stored in giving up in fluid reservoir 5 In liquid storage tank 9 and liquid feeding tank 10, opening upper cover 3 can be replaced and be added to waste tank 9 and liquid feeding tank 10, when needs are to training When foster ware 137 carries out changing liquid and addition, the movement of bogey 13 is controlled by PLC controller 16, culture dish 137 is turned to and is changed The bottom of liquid device 12, specifically, PLC controller 16 sends control signal, driving motor 131 passes through 132 He of train of reduction gears Conical gear group 133 is driven the rotation for realizing support plate 136, and array distribution has culture dish 137 everywhere, support plate 136 on support plate 136 An angle of 90 degrees is rotated every time, culture dish 137 is made to turn to the bottom of drain pipe 121 and liquid-feeding tube 124, lifting gear 11 controls at this time The movement of liquid changing device 12 changes liquid and addition, specifically, lifting motor 113 passes through by the realization culture solution of liquid changing device 12 The transmission of screw rod 117 and the block 116 that is spirally connected, and vertical lift up and down is realized under the guiding role of guide post 114 and slide 115, it will Drainage head 122 and liquid feeding first 125 are stretched into culture dish 137, control positive displacement pump 123 will be in culture dish 137 by PLC controller 16 Culture solution is extracted and kept in waste tank 9, and then PLC controller 16 controls charging valve 26 to open, the training in liquid feeding tank 10 Nutrient solution is flowed by liquid-feeding tube 124 in culture dish 137, and electronic flowmeter 127 monitors flow, and communicates information to PLC controls Device 16 processed, when completing liquid feeding, PLC controller 16 controls charging valve 126 to close, so as to fulfill the replacement of culture solution;This practicality is new Type is equipped with heating wire 7 on the inner wall of culturing room 6, and is controlled by PLC come monitoring temperature information by temperature sensor 8 Device 16 controls the work of heating wire 7 to carry out regulation culture temperature, and the insulating layer 2 of rock wool material is equipped on 1 inner wall of babinet, is conducive to The constant of cultivation temperature is kept, and reduces heat and scatters and disappears, reduces energy consumption.
The opening in technical solutions of the utility model using technical solution described in the utility model or those skilled in the art It gives, designs similar technical solution, and reach above-mentioned technique effect, be to fall into the scope of protection of the utility model.

Claims (8)

1. a kind of medical experiment incubator of automatically replaceable culture solution, it is characterised in that:The medical experiment is used can automatically more Change culture solution incubator include babinet, insulating layer, upper cover, partition plate, fluid reservoir, culturing room, heating wire, temperature sensor, give up Liquid storage tank, liquid feeding tank, lifting gear, column, fixed plate, lifting motor, guide post, slide, be spirally connected block, screw rod, bearing, changes liquid dress It puts, drain pipe, drainage head, positive displacement pump, liquid-feeding tube, liquid feeding head, charging valve, electronic flowmeter, drip plate, bogey, drives Motor, train of reduction gears, conical gear group, pedestal, bearing, support plate, culture dish, locating piece, locating slot, chamber door, observation window and PLC controller;The madial wall of the babinet is equipped with insulating layer, and the insulating layer is heatproof rock wool material;The babinet it is interior Portion is separated to form two chambers above and below fluid reservoir and culturing room by partition plate;The waste tank and liquid feeding tank are arranged on fluid reservoir It is interior;The top of the waste tank and liquid feeding tank is equipped with upper cover in babinet;The temperature sensor is installed on the bottom of partition plate; The outside of the culturing room by hinge on babinet in being hinged with chamber door;The chamber door is equipped with observation window;The PLC controller On the outer wall of babinet;The lifting gear is arranged in culturing room;The lifting gear includes column, fixed plate, lifting electricity Machine, guide post, slide, the block that is spirally connected, screw rod and bearing;The fixed plate is fixedly installed in the top side of column;The lifting motor peace Top loaded on fixed plate;The power output end of the lifting motor is connected with screw rod;The bottom of the fixed plate is in screw rod Both sides be equipped with two at guide post;The slide is sheathed on guide post;The block that is spirally connected is sheathed on screw rod;Block and the cunning of being spirally connected Seat is an integral structure;The right end of the slide is equipped with the bearing of integral type;The liquid changing device is installed on bearing;It is described to change Liquid device includes drain pipe, drainage head, positive displacement pump, liquid-feeding tube, liquid feeding head, charging valve, electronic flowmeter and drip plate;The row Liquid pipe and liquid-feeding tube are arranged on bearing;The bottom of the drain pipe and liquid-feeding tube is respectively equipped with drainage head and liquid feeding head;Institute The top for stating drain pipe and liquid-feeding tube is connected respectively with waste tank and liquid feeding tank;The drain pipe is equipped with positive displacement pump;Institute Liquid-feeding tube is stated equipped with charging valve and electronic flowmeter;The bogey is arranged on the bottom of liquid changing device;The bogey Including driving motor, train of reduction gears, conical gear group, pedestal, bearing, support plate, culture dish, locating piece and locating slot;It is described Driving motor is connected by train of reduction gears with conical gear group;The top of the pedestal is turned by bearing and conical gear group Dynamic connection;The support plate is fixedly installed on the top of conical gear group;It is described that locating piece everywhere is distributed in the top array done; The bottom of the culture dish is equipped with locating slot;It is in mating connection that the culture dish by the locating slot of bottom is installed on locating piece.
2. the medical experiment as described in claim 1 incubator of automatically replaceable culture solution, it is characterised in that:The drainage head It is semicircular arc structure with liquid feeding head.
3. the medical experiment as claimed in claim 1 or 2 incubator of automatically replaceable culture solution, it is characterised in that:The row The bottom of liquid head and liquid feeding head is equipped with drip plate.
4. the medical experiment as described in claim 1 incubator of automatically replaceable culture solution, it is characterised in that:The culture dish Drainage head is right against in the position on support plate and liquid feeding head is set.
5. the medical experiment as described in claim 1 incubator of automatically replaceable culture solution, it is characterised in that:The observation window Culture dish setting is right against on horizontal level.
6. the medical experiment as described in claim 1 incubator of automatically replaceable culture solution, it is characterised in that:The drain pipe It is hose that helical form is set to be located at one section of holder top with liquid-feeding tube, and one section positioned at rest base is plastic rigid pipe.
7. the medical experiment as described in claim 1 incubator of automatically replaceable culture solution, it is characterised in that:The heating wire S-type structure is wound on the inner wall of culturing room.
8. the medical experiment as described in claim 1 incubator of automatically replaceable culture solution, it is characterised in that:The charging valve For electric-controlled type valve.
CN201721368660.0U 2017-10-23 2017-10-23 A kind of incubator of the automatically replaceable culture solution of medical experiment Expired - Fee Related CN207435459U (en)

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CN201721368660.0U CN207435459U (en) 2017-10-23 2017-10-23 A kind of incubator of the automatically replaceable culture solution of medical experiment

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Application Number Priority Date Filing Date Title
CN201721368660.0U CN207435459U (en) 2017-10-23 2017-10-23 A kind of incubator of the automatically replaceable culture solution of medical experiment

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108641956A (en) * 2018-06-19 2018-10-12 张起 A kind of adherent type cell culture system
CN112300935A (en) * 2020-11-05 2021-02-02 广东康盾生物工程技术有限公司 Stem cell isolated culture device

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108641956A (en) * 2018-06-19 2018-10-12 张起 A kind of adherent type cell culture system
CN112300935A (en) * 2020-11-05 2021-02-02 广东康盾生物工程技术有限公司 Stem cell isolated culture device

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Granted publication date: 20180601

Termination date: 20181023