CN108641956A - A kind of adherent type cell culture system - Google Patents
A kind of adherent type cell culture system Download PDFInfo
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- CN108641956A CN108641956A CN201810629517.5A CN201810629517A CN108641956A CN 108641956 A CN108641956 A CN 108641956A CN 201810629517 A CN201810629517 A CN 201810629517A CN 108641956 A CN108641956 A CN 108641956A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/04—Flat or tray type, drawers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
Abstract
The present invention provides a kind of adherent type cell culture system, including universal wheel, culture solution entrance, culture solution entry switch, culture solution pipeline, box body device, incubator support plate device, control device, central control unit, liquid feeding entrance, liquid feeding entry switch and charging line, there are four the bottoms for being threaded in box body device for the universal wheel；The culture solution entrance is located at the lower left side position of the sealing cover of box body device；The culture solution entry switch is mounted on the lower part of culture solution entrance；The control device is located at the leftward position of box body device inner wall；By the setting of central control unit, there is the growth situation for facilitating real time monitoring cell, advantage easy to use；By the setting of box body device, the infringement of germ can be prevented, is also convenient for setting culture solution pipeline and charging line, saves space, it is easy to use；By the setting of incubator support plate device, the infringement of germ can be prevented, is also convenient for setting culture solution pipeline and charging line, saves space, it is easy to use.
Description
Technical field
The invention belongs to technical field of cell culture more particularly to a kind of adherent type cell culture systems.
Background technology
Cell is the base unit of the morphosis of all living things body and vital movement in addition to virus.Human body is no exception,
Various types of cells constitutes the various tissues and organ of human body, and carries human body from fertilization, birth to growth and development, until dead
The whole vital movements for the whole life cycle died.All physiological function and biochemical reaction in vivo, be all in these cells and
Being carried out on the material base of its product (such as the collagen and proteoglycans in space between cells).Cell be in addition to virus all
The morphosis of organism and the base unit of vital movement.Human body is no exception, in these vital movements, all cells
All in different environment, body provides an environment stable state for the benign growths of cell.
China Patent Publication No. is 106085858 A of CN, the entitled adherent type cell culture system of innovation and creation and side
The liquid outlet of method, including cell culture case, device for storing liquid and power plant, the device for storing liquid is trained by pipeline and the cell
The inlet connection of box is supported, the liquid outlet of the cell culture case is connect by pipeline with the inlet of the device for storing liquid, institute
It states power plant to be set on the pipeline, for driving the media flow in the cell culture case.The system has
It is simple in structure, easy to use, the characteristics of mass cell culture can be carried out.But existing adherent type cell culture system is also deposited
It is inconvenient to use simple in structure, the shortcomings that cannot monitoring in real time.
Therefore, a kind of adherent type cell culture system of invention is very necessary.
Invention content
In order to solve the above technical problem, the present invention provides a kind of adherent type cell culture systems, to solve existing structure
Simply, inconvenient to use, the shortcomings that cannot monitoring in real time.A kind of adherent type cell culture system, including universal wheel, culture solution enter
Mouth, culture solution entry switch, culture solution pipeline, box body device, incubator support plate device, control device, central control unit add
Liquid entrance, liquid feeding entry switch and charging line, the universal wheel are set as four, are filled mounted on babinet by screw thread
The four corners set；The culture solution entrance is located at the upper left-hand position of box body device；The culture solution entry switch peace
Mounted in the lower part of culture solution entrance；The culture solution pipeline is located in the filter layer of box body device, is connected to culture solution entrance
The lower part of switch, and bottom is reserved with liquid outlet；What described incubator support plate device one end was connected to culture solution pipeline goes out liquid
On mouth, the other end is connected on the filling opening of charging line；The control device is located at the leftward position of box body device inner wall；
The central control unit is mounted on the centre position on the top of the sealing cover of box body device；The liquid feeding entrance is located at case
The upper right position of body device；The liquid feeding entry switch is mounted on the lower position of liquid feeding entrance；The liquid-feeding tube
Road is located in the filter layer of box body device, is connected to the lower part of liquid feeding entry switch, and bottom is reserved with liquid outlet.
The control device includes carbon dioxide steel cylinder, automatic sensing valve, concentration inductor, temperature inductor, humidity
Inductor, environmental monitor, electrical heating and without freon compressing unit, the carbon dioxide steel cylinder is located at the right side of incubator support plate device
Upper lateral part position；The automatic sensing valve is mounted at the upper switches of carbon dioxide steel cylinder, and is electrically connected at central control
Device processed；The concentration inductor is mounted on the lower position of carbon dioxide steel cylinder, and is electrically connected at central control unit；
The temperature inductor is mounted on the lower position of concentration inductor, and is electrically connected at central control unit；Described is wet
It spends inductor and is mounted on the lower position of temperature inductor, and be electrically connected at central control unit；The environmental monitor
Mounted on the lower position of humidity sensor, and it is electrically connected at central control unit；The electrical heating is supervised mounted on environment
The lower position of device position is surveyed, and is electrically connected at central control unit；It is described without freon compressing unit be mounted on it is electrically heated under
Portion position, and be electrically connected at central control unit, have it is reasonable for structure, it is easy to use, facilitate adjustment cell growth to need
The advantages of temperature, environment, nutrient solution situation.
The central control unit includes processor, electrical heating module, automatic sensing valve module, concentration inductor mould
Block, temperature inductor module, humidity sensor module, environmental monitor module, floride-free compressor module, elevator module mould
Block, external power supply, internal electric source, display module and display, the processor are the core of central control unit, and pass through
Conducting wire and electrical heating module, automatic sensing valve module, concentration sensor module, temperature inductor module, humidity sensor module,
Environmental monitor module, floride-free compressor module are connected with elevator module；And one end of internal electric source is connected with external power supply；
The display module is connected by conducting wire with display, has the growth situation for facilitating real time monitoring cell, easy to use
Advantage.
The box body device includes sealing cover, air inlet, filtering pading layer, leakage fluid dram and leakage fluid dram switch, and described is close
Capping is mounted on the top of box body device, and sealing cover upper middle position is central control unit, and there is rubber pad at four wall edges
Circle；The air inlet is located at the bottom left position of box body device, and inside is equipped with filter screen；The filtering pading layer peace
Mounted in the centre position of the inner and outer wall of box body device；The leakage fluid dram is located at the right positions of the bottom of box body device,
And it is connected to the lower position of leakage fluid dram switch, it is connected on the reserved leakage fluid dram of incubator support plate device；The leakage fluid dram
Switch can prevent the infringement of germ positioned at the upper position of leakage fluid dram, be also convenient for setting culture solution pipeline and charging line, section
Save space, it is easy to use.
The incubator support plate device includes culture plate, culture dish, built-in pipe, culture dish pipeline, culture plate bottom
Card slot, pallet stretch pull rod, elevator and Drainage pipe, and the elevator is mounted on the interposition of box body device inner wall bottom
It sets；The upper position stretched pull rod and be located at elevator；The pallet is mounted on the upper position for stretching pull rod；The training
It supports board bottom portion card slot and is located at the bottom of culture plate, and culture plate bottom card slot size and pallet is in the same size；The culture
Ware is mounted on culture plate, and size is identical, is evenly arranged on culture plate, and the built-in pipe is located at the interposition of culture plate
It sets；The culture dish pipeline is connected to the centre position of culture dish and built-in pipe；The Drainage pipe is connected to built-in
The right positions of pipeline；The culture plate is connected to the upper position of elevator, can cultivate multiple cells, can be according to thin
Born of the same parents, which grow up, is added the addition of nutrient solution and other liquid.
The automatic sensing valve uses E3JK-DS30M1 switch sensors, can be replenished in time in cells grown and need
Carbon dioxide.
The concentration inductor uses MJR25SX inductors, can monitor the air concentration feelings in cellular environment in real time
Condition.
The temperature inductor uses MAX44009 inductors, can monitor the temperature feelings during cells grown in real time
Condition.
The humidity sensor uses MODBUS inductors, can monitor the humidity feelings during cells grown in real time
Condition.
The environmental monitor uses AIRNOW detectors, can monitor the pollution feelings of air in cells grown in real time
Condition.
Described uses GTM12AA environmental protection R134A compressors without freon compressing unit, has free-floride, and noise is small, and refrigeration is rapid.
Compared with prior art, the present invention has the advantages that：
1. the setting of control device of the present invention has reasonable for structure, easy to use, the temperature for facilitating adjustment cell growth to need
The advantages of degree, environment, nutrient solution situation.
2. the setting of the central control unit of the present invention has the growth situation for facilitating real time monitoring cell, easy to use
The advantages of.
3. the setting of the box body device of the present invention, can prevent the infringement of germ, it is also convenient for setting culture solution pipeline and adds
Space is saved in liquid pipe road, easy to use.
4. the setting of the incubator support plate device of the present invention, can cultivate multiple cells, can be added according to cells grown
The addition of nutrient solution and other liquid.
Description of the drawings
Fig. 1 is the structural schematic diagram of the present invention.
Fig. 2 is the control device structural schematic diagram of the present invention.
Fig. 3 is the central control unit structural schematic diagram of the present invention.
Fig. 4 is the box body device structural schematic diagram of the present invention.
Fig. 5 is the incubator support plate apparatus structure schematic diagram of the present invention.
In figure：
1- universal wheels, 2- culture solution entrances, 3- culture solution entry switches, 4- culture solution pipelines, 5- box body devices, 51- are close
Capping, 52- air inlets, 53- filtering pading layers, 54- leakage fluid drams, 55- leakage fluid drams switch, 6- incubator support plate devices, 61- cultures
Plate, 62- culture dishes, 63- built-in pipes, 64- culture dish pipelines, 65- culture plates bottom card slot, 66- pallets, 67- stretch pull rod,
68- elevators, 7- control devices, 71- carbon dioxide steel cylinders, 72- automatic sensing valves, 73- concentration inductors, 74- temperature senses
Device, 75- humidity sensors, 76- environmental detectors, 77- electrical heating, 78- is without freon compressing unit, 8- central control units, 81- processing
Device, 82- electrical heating modules, 83- automatic sensing valve modules, 84- concentration sensor modules, 85- temperature inductor modules, 86- are wet
Degree sensor module, 87- environmental detector modules, 88- free-floride compressor modules, 89- elevator modules, 810- external power supplies,
811- internal electric sources, 812- display modules, 813- displays, 9- liquid feeding entrances, 10- heated inlets switch, 11- charging lines.
Specific implementation mode
The present invention is described further below in conjunction with attached drawing：
Embodiment：
As shown in attached drawing 1 to attached drawing 5
The present invention provides a kind of adherent type cell culture system, including universal wheel 1, culture solution entrance 2, culture solution entrance are opened
Pass 3, culture solution pipeline 4, box body device 5, incubator support plate device 6, control device 7, central control unit 8, liquid feeding entrance 9,
Liquid feeding entry switch 10 and charging line 11, the universal wheel 1 are set as four, are connected through a screw thread mounted on box body device 5
Four corners；The culture solution entrance 2 is located at 5 upper left-hand position of box body device；The culture solution entry switch 3 is installed
In the lower part of culture solution entrance 2；The culture solution pipeline 4 is located in the filter layer of box body device 5, is connected to culture solution entrance
The lower part of switch 3, and bottom is reserved with liquid outlet；Described 6 one end of incubator support plate device is connected to going out for culture solution pipeline 4
On liquid mouth, the other end is connected on the filling opening of charging line 11；The control device 7 is located at a left side for 5 inner wall of box body device
Side position；The central control unit 8 is mounted on the upper middle position of the sealing cover 51 of box body device 5；The liquid feeding
Entrance 9 is located at the upper right position of box body device 5；The liquid feeding entry switch 10 is mounted on the lower portion of liquid feeding entrance 9
It sets；The charging line 11 is located in the filter layer of box body device 5, is connected to the lower part of liquid feeding entry switch 10, and bottom
It is reserved with liquid outlet.
The control device 7 includes carbon dioxide steel cylinder 71, automatic sensing valve 72, concentration inductor 73, temperature inductor
74, humidity sensor 75, environmental monitor 76, electrical heating 77 and without freon compressing unit 78, the carbon dioxide steel cylinder 71 is located at
The right upper portion position of incubator support plate device 6；It opens on the top that the automatic sensing valve 72 is mounted on carbon dioxide steel cylinder 71
At pass, and it is electrically connected at central control unit 8；The concentration inductor 73 is mounted on the lower portion of carbon dioxide steel cylinder 71
It sets, and is electrically connected at central control unit 8；The temperature inductor 74 is mounted on the lower position of concentration inductor 73,
And it is electrically connected at central control unit 8；The humidity sensor 75 is mounted on the lower position of temperature inductor 74, and electricity
Property is connected to central control unit 8；The environmental monitor 76 is mounted on the lower position of humidity sensor 75, and electrically connects
It is connected on central control unit 8；The electrical heating 77 is mounted on the lower position of 76 position of environmental monitor, and is electrically connected at
Central control unit 8；Described is mounted on the lower position of electrical heating 77 without freon compressing unit 78, and is electrically connected at central control
Device 8, have it is reasonable for structure, it is easy to use, facilitate adjustment cell growth need temperature, environment, nutrient solution situation the advantages of.
The central control unit 8 includes processor 81, electrical heating module 82, automatic sensing valve module 83, concentration sense
Answer device module 84, temperature inductor module 85, humidity sensor module 86, environmental monitor module 87, floride-free compressor module
88, elevator module 89, external power supply 810, internal electric source 811, display module 812 and display 813, the processing
Device 81 is the core of central control unit 8, and passes through conducting wire and electrical heating module 82, automatic sensing valve module 83, concentration induction
Device module 84, temperature inductor module 85, humidity sensor module 86, environmental monitor module 87, floride-free compressor module 88
It is connected with elevator module 89；And one end of internal electric source 811 is connected with external power supply 810；The display module 812 passes through
Conducting wire is connected with display 813, has the growth situation for facilitating real time monitoring cell, advantage easy to use.
The box body device 5 includes sealing cover 51, air inlet 52, filtering pading layer 53, leakage fluid dram 54 and leakage fluid dram switch
55, the sealing cover 51 is mounted on the top of box body device 5, and sealing cover upper middle position is central control unit 8, and four
There is rubber washer at wall edge；The air inlet 52 is located at the bottom left position of box body device 5, and inside is equipped with filtering
Net；The filtering pading layer 53 is mounted on the centre position of the inner and outer wall of box body device；The leakage fluid dram 54 is located at case
The right positions of the bottom of body device 5, and it is connected to the lower position of leakage fluid dram switch 55, it is connected to incubator support plate device 6
On reserved leakage fluid dram；The leakage fluid dram switch 55 is located at the upper position of leakage fluid dram 54, can prevent the infringement of germ, also
Convenient for setting culture solution pipeline and charging line, space is saved, it is easy to use.
The incubator support plate device 6 includes culture plate 61, culture dish 62, built-in pipe 63, culture dish pipeline 64, training
Board bottom portion card slot 65 is supported, pallet 66 stretches pull rod 67, elevator 68 and Drainage pipe 69, and the elevator 68 is mounted on babinet
The centre position of 5 inner wall bottom of device；The upper position stretched pull rod 67 and be located at elevator 68；The pallet 66 is installed
In the upper position for stretching pull rod 67；The culture plate bottom card slot 65 is located at the bottom of culture plate, and culture plate bottom card slot
65 sizes and pallet 66 it is in the same size；The culture dish 62 is mounted on culture plate 61, and size is identical, is evenly arranged in training
It supports on plate 61, the built-in pipe 63 is located at the centre position of culture plate 61；The culture dish pipeline 64 is connected to culture
The centre position of ware 62 and built-in pipe 63；The Drainage pipe 69 is connected to the right positions of built-in pipe 63；Described
Culture plate 61 is connected to the upper position of elevator 68.6, a kind of adherent type cell culture system as claimed in claim 2,
It is characterized in that：The automatic sensing valve 72 uses E3JK-DS30M1 switch sensors, can cultivate multiple cells, being capable of basis
The addition of nutrient solution and other liquid is added in cells grown.
The automatic sensing valve 72 uses E3JK-DS30M1 switch sensors, and can be replenished in time in cells grown needs
The carbon dioxide wanted.
The concentration inductor 73 uses MJR25SX inductors, can monitor the air concentration in cellular environment in real time
Situation.
The temperature inductor 74 uses MAX44009 inductors, can monitor the temperature during cells grown in real time
Situation.
The humidity sensor 75 uses MODBUS inductors, can monitor the humidity feelings during cells grown in real time
Condition.
The environmental monitor 76 uses AIRNOW detectors, can monitor the pollution feelings of air in cells grown in real time
Condition.
Described uses GTM12AA environmental protection R134A compressors without freon compressing unit, has free-floride, and noise is small, and refrigeration is rapid.
Operation principle
In the present invention, the sealing cover 51 of box body device 5 is opened, cell is put into culture dish 62, and drawing is stretched in the drive of elevator 68
Bar 67 moves downward, and stretches pull rod 67 and pallet 66 is driven to move downward, pallet drives culture plate 61 to be moved downwardly to culture solution pipeline 4
On the interface of charging line 11, culture solution entry switch 3 is opened, culture is added through culture solution entrance 2 and culture solution pipeline 4
Liquid, culture solution are exported from culture solution pipeline 4 and are flowed into the built-in pipe 63 on culture plate 61, and the nutrient solution in built-in pipe 63 is logical
Culture dish pipeline 64 is crossed, culture dish 62 is flowed into full culture solution, while mounted on the certainly dynamic of 71 upper switches of carbon dioxide steel cylinder
Valve 72 is answered, concentration inductor 73, temperature inductor 74, humidity sensor 75 and environmental monitor 76 pass through central control unit 8
It starts to work, while electrical heating 77 and the environmental work that is needed according to cell without freon compressing unit 78, while central control unit 8 is led to
Cross the ambient conditions that display 813 monitors cells grown.
Using technical solution of the present invention or those skilled in the art under the inspiration of technical solution of the present invention, if
Similar technical solution is counted out, and reaches above-mentioned technique effect, is to fall into protection scope of the present invention.
Claims (11)
1. a kind of adherent type cell culture system, it is characterised in that：Including universal wheel (1), culture solution entrance (2), culture solution enters
Mouth switch (3), culture solution pipeline (4), box body device (5), incubator support plate device (6), control device (7), center control dress
It sets (8), liquid feeding entrance (9), liquid feeding entry switch (10) and charging line (11), the universal wheel (1) is set as four, respectively
It is connected through a screw thread the four corners mounted on box body device (5)；The culture solution entrance (2) is located at the upper of box body device (5)
Portion's leftward position；The culture solution entry switch (3) is mounted on the lower part of culture solution entrance (2)；The culture solution pipeline
(4) it is located in the filter layer of box body device (5), is connected to the lower part of culture solution entry switch (3), and bottom is reserved with out liquid
Mouthful；Described incubator support plate device (6) one end is connected on the liquid outlet of culture solution pipeline (4), and the other end is connected to liquid feeding
On the filling opening of pipeline (11)；The control device (7) is located at the leftward position of box body device (5) inner wall；The center
Control device (8) is mounted on the centre position on the top of the sealing cover (51) of box body device (5)；Described liquid feeding entrance (9) position
Right positions in the top of box body device (5)；The liquid feeding entry switch (10) is mounted on the lower portion of liquid feeding entrance (9)
It sets；The charging line (11) is located in the filter layer of box body device (5), is connected to the lower part of liquid feeding entry switch (10),
And bottom is reserved with liquid outlet.
2. a kind of adherent type cell culture system as described in claim 1, it is characterised in that：Control device (7) packet
Include carbon dioxide steel cylinder (71), automatic sensing valve (72), concentration inductor (73), temperature inductor (74), humidity sensor
(75), environmental monitor (76), electrical heating (77) and without freon compressing unit (78), the carbon dioxide steel cylinder (71) is located at culture
The right upper portion position of device support plate device (6)；The automatic sensing valve (72) is mounted on the top of carbon dioxide steel cylinder (71)
At switch, and it is electrically connected at central control unit (8)；The concentration inductor (73) is mounted on carbon dioxide steel cylinder (71)
Lower position, and be electrically connected at central control unit (8)；The temperature inductor (74) is mounted on concentration inductor
(73) lower position, and it is electrically connected at central control unit (8)；The humidity sensor (75) is mounted on temperature sense
The lower position of device (74), and it is electrically connected at central control unit (8)；The environmental monitor (76) is mounted on humidity sense
The lower position of device (75) is answered, and is electrically connected at central control unit (8)；The electrical heating (77) is mounted on environmental monitoring
The lower position of device (76) position, and it is electrically connected at central control unit (8)；Described is mounted on electricity without freon compressing unit (78)
The lower position of (77) is heated, and is electrically connected at central control unit (8).
3. a kind of adherent type cell culture system as described in claim 1, it is characterised in that：The central control unit
(8) include processor (81), electrical heating module (82), automatic sensing valve module (83), concentration sensor module (84), temperature sense
Answer device module (85), humidity sensor module (86), environmental monitor module (87), floride-free compressor module (88), elevator
Module (89), external power supply (810), internal electric source (811), display module (812) and display (813), the processor
(81) it is the core of central control unit (8), and by conducting wire and electrical heating module (82), automatic sensing valve module (83) is dense
Spend sensor module (84), temperature inductor module (85), humidity sensor module (86), environmental monitor module (87), nothing
Freon compressing unit module (88) is connected with elevator module (89)；And one end and external power supply (810) phase of internal electric source (811)
Even；The display module (812) is connected by conducting wire with display (813).
4. a kind of adherent type cell culture system as described in claim 1, it is characterised in that：Box body device (5) packet
Include sealing cover (51), air inlet (52), filtering pading layer (53), leakage fluid dram (54) and leakage fluid dram switch (55), the sealing cover
(51) it is mounted on the top of box body device (5), sealing cover upper middle position is central control unit (8), and four wall edges have
Rubber washer；The air inlet (52) is located at the bottom left position of box body device (5), and inside is equipped with filter screen；Institute
The filtering pading layer (53) stated is mounted on the centre position of the inner and outer wall of box body device；The leakage fluid dram (54) is located at babinet
The right positions of the bottom of device (5), and it is connected to the lower position of leakage fluid dram switch (55), it is connected to incubator support plate device
(6) on reserved leakage fluid dram；The leakage fluid dram switch (55) is located at the upper position of leakage fluid dram (54).
5. a kind of adherent type cell culture system as described in claim 1, it is characterised in that：The incubator support plate device
(6) include culture plate (61), culture dish (62), built-in pipe (63), culture dish pipeline (64), culture plate bottom card slot (65),
Pallet (66), stretches pull rod (67), elevator (68) and Drainage pipe (69), and the elevator (68) is mounted on box body device
(5) centre position of inner wall bottom；The upper position stretched pull rod (67) and be located at elevator (68)；The pallet (66)
Mounted on the upper position for stretching pull rod (67)；The culture plate bottom card slot (65) is located at the bottom of culture plate, and culture plate
Bottom card slot (65) size and pallet (66) it is in the same size；The culture dish (62) is mounted on culture plate (61), size
It is identical, it is evenly arranged on culture plate (61), the built-in pipe (63) is located at the centre position of culture plate (61)；Described
Culture dish pipeline (64) is connected to the centre position of culture dish (62) and built-in pipe (63)；Drainage pipe (69) connection
Right positions in built-in pipe (63)；The culture plate (61) is connected to the upper position of elevator (68).
6. a kind of adherent type cell culture system as claimed in claim 2, it is characterised in that：The automatic sensing valve (72)
Using E3JK-DS30M1 switch sensors.
7. a kind of adherent type cell culture system as claimed in claim 2, it is characterised in that：The concentration inductor (73)
Using MJR25SX inductors.
8. a kind of adherent type cell culture system as claimed in claim 2, it is characterised in that：The temperature inductor (74)
Using MAX44009 inductors.
9. a kind of adherent type cell culture system as claimed in claim 2, it is characterised in that：The humidity sensor (75)
Using MODBUS inductors.
10. a kind of adherent type cell culture system as claimed in claim 2, it is characterised in that：The environmental monitor
(76) AIRNOW detectors are used.
11. a kind of adherent type cell culture system as claimed in claim 2, it is characterised in that：It is described without freon compressing unit
(78) GTM12AA environmental protection R134A compressors are used.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810629517.5A CN108641956A (en) | 2018-06-19 | 2018-06-19 | A kind of adherent type cell culture system |
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| CN201810629517.5A CN108641956A (en) | 2018-06-19 | 2018-06-19 | A kind of adherent type cell culture system |
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| CN108641956A true CN108641956A (en) | 2018-10-12 |
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| WO2017059273A1 (en) * | 2015-10-01 | 2017-04-06 | Berkeley Lights, Inc. | Well-plate incubator |
| CN207435459U (en) * | 2017-10-23 | 2018-06-01 | 中国医科大学附属盛京医院 | A kind of incubator of the automatically replaceable culture solution of medical experiment |
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2018
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Application publication date: 20181012 |