CN207294795U - Edible oil DNA filters extraction element - Google Patents

Abstract

The utility model discloses edible oil DNA to filter extraction element,Including housing,Weight display,Trapped fuel chamber,Active plugboard,First strainer,Silica gel filter membrane,Second strainer,Discharge pipe,Strong suction pumps,Placing groove,Store bucket,First weight sensor,Placement rack,Second weight sensor,Temperature controller,Heater,Rotate lid,Escape groove,Placing tube,Suction pump,Suction pipe,Connecting tube,Edible oil DNA filters extraction element,Clever structure,It is powerful,Pass through micropore separating mechanism first,It can realize the stop separating treatment to DNA fragmentation and oil molecule,Secondly by negative suction system,Persistently oil molecule can be aspirated,Then the separating rate of DNA fragmentation and oil molecule is promoted,Eventually through above-mentioned,So as to while realizing to DNA fragmentation high efficiency extraction in edible oil,Also the operating burden of staff is greatly alleviated,Beneficial to the popularization and application of industry.

Description

Edible oil DNA filters extraction element

Technical field

It the utility model is related to extraction technical field of experiment equipment, more particularly to edible oil DNA filtering extraction elements.

Background technology

China mainly has currently used for the transgenosis raw material of processing and eating oil：Soybean, corn, rapeseed, most is to turn Transgenic soybean.

Food hygiene management mechanism of various countries takes this attitude of extreme discretion invariably, is required to food processing, production enterprise Industry does clear and definite mark in packaging for foodstuff to the service condition of transgenic product.Which make explicit consumer understand in food to wrap Transgenic product is included, so as to make voluntary selection.But whether contain in food, containing which type of transgene component simultaneously Non- is open-and-shut thing.Even developed with current biotechnology, to the sample mixed with a small amount of transgenic product, be particularly By in the food of deep processing, whether the detection containing transgene component is still an intractable problem.Lack effective detection Technology, just can not effectively supervise commercial product.Relevant laws and regulations also just perform practically no function.Therefore, develop it is sensitive, Efficient detection GMOs technology has become one of important topic of current biological research methods research.

The existing home and abroad technical literature on the extraction of edible oil DNA fragmentation is seldom.Its general technical route mostly be first with Cetyl trimethylammonium bromide (CTAB) will precipitate for the lysate of main component to be dissolved, then filtered, so as to fulfill oil Son and the separation of DNA fragmentation, but since edible oil is deep processing grease, the effective dna fragment in edible oil is seldom,

Operation traditional at present, lacks efficient separator, causes complex steps, time and effort consuming, so as to work Personnel's operation brings greatly inconvenience at the same time, also to have impact on the extraction effect of DNA fragmentation, in view of disadvantages described above, it is really necessary to set Count edible oil DNA filtering extraction elements.

Utility model content

The technical problem to be solved by the utility model is to：Edible oil DNA filtering extraction elements are provided, to solve background The problem of technology proposes.

In order to solve the above technical problems, the technical solution of the utility model is：Edible oil DNA filters extraction element, including Housing, weight display, trapped fuel chamber, active plugboard, the first strainer, silica gel filter membrane, the second strainer, discharge pipe, strong suction are pumped, put Put groove, storage bucket, the first weight sensor, placement rack, the second weight sensor, temperature controller, heater, rotate lid, escape groove, Placing tube, suction pump, suction pipe, connecting tube, the weight display are located at housing exterior walls upper end, the weight display It is connected with housing screw thread, the trapped fuel chamber is located at enclosure interior upper end, and the trapped fuel chamber is rectangular cavities, the activity Plate runs through enclosure interior middle-end, and the active plugboard is connected with housing buckle, and first strainer is located at active plugboard Inner upper end, first strainer are connected with active plugboard screw thread, and the silica gel filter membrane is located at middle-end inside active plugboard, The silica gel filter membrane is movably connected with active plugboard, and second strainer is located at active plugboard interior lower end, and described Two strainers are connected with active plugboard screw thread, and the discharge pipe runs through enclosure interior middle-end, the discharge pipe and housing screw thread It is connected, strong suction pump is located at discharge pipe middle-end, and strong suction pump is connected with discharge pipe screw thread, and the strong suction Power pump is connected with housing screw thread, and the placing groove is located at enclosure interior front end downside, and the placing groove is rectangular recess, institute Inside placing groove, the storage bucket is movably connected the storage bucket position stated with housing, and first weight sensor is located at Bottom on the right side of enclosure interior, first weight sensor are connected with housing screw thread, and first weight sensor and Weight display conducting wire is connected, and the placement rack is located at lower end on the right side of housing, and the placement rack is connected with housing screw thread, institute The second weight sensor stated is located at placement rack inside left bottom, second weight sensor and placement rack screw thread phase Even, and second weight sensor is connected with weight display conducting wire, and the temperature controller is located at placement rack outer wall upper end Right side, the temperature controller are connected with placement rack screw thread, and the heater is located at placement rack inner right side, the heater It is connected with placement rack screw thread, and the heater is connected with temperature controller conducting wire, the rotation lid is located at the top of placement rack, institute It is rotatedly connected on the right side of the rotation lid stated with placement rack, the escape groove is positioned at rotation lid inside left middle-end, the avoidance Groove is rectangular recess, and the placing tube is located at placement rack inside left, and the placing tube is movably connected with placement rack, described Suction pump be located at upper end on the right side of housing, the suction pump is connected with housing screw thread, and the suction pipe is located at suction pump bottom Portion, the suction pipe are connected with suction pump screw thread, and the connecting tube is through upper end on the right side of enclosure interior, the connecting tube One end is connected with housing screw thread, and the connecting tube other end is connected with suction pump screw thread.

Further, cabinet is additionally provided with the left of the enclosure interior, the cabinet is movably connected with housing.

Further, the housing right hand top is additionally provided with cover board, and the cover board is movably connected with housing.

Further, holder is additionally provided with the left of the case top, the holder is movably connected with housing.

Further, the placement rack inner right side is additionally provided with protection network, and the protection network is connected with placement rack screw thread.

Further, a number of micropore is also equipped with inside the silica gel filter membrane, the micro-pore diameter is 2um- 4um。

Compared with prior art, edible oil DNA filters extraction element, and before extraction, staff is by weight display, the One weight sensor, the second weight sensor, strong suction pump, suction pump, temperature controller and heater and extraneous power lead phase Connect, then open and rotate lid, then the placing tube equipped with edible oil and 2%CTAB extraction buffers is inserted into placement rack, and close It is upper to rotate lid, then by suction pipe from escape groove through insertion placing tube inside, during extraction, staff is first turned on aspirating Pump, i.e., open the effect that suction is produced to suction pipe by suction pump, realizes placing tube inside edible oil by suction pipe by even Adapter flows into trapped fuel intracavitary, and then edible oil is wandered to the first strainer, and because of the effect of the first strainer, edible oil can be carried out Preliminary filtering, then edible oil is wandered to silica gel filter membrane, because being distributed the micro- of a number of diameter 2um-4um inside silica gel filter membrane Hole, i.e., by the effect of micropore, realize the stop to extracting DNA fragmentation needed for edible oil inside, meanwhile, staff opens strong Suction pump, i.e., by producing the effect of strong negative-pressure suction after strong suction pump work, the edible oil above silica gel filter membrane carries out Suction downwards, at this time by above-mentioned, the oil molecule for accelerating to be less than inside edible oil micro-pore diameter passes through silica gel filter membrane and second The speed that strainer is wandered downwards, meanwhile, it is blocked more than micro-pore diameter DNA fragmentation, eventually through above-mentioned, so as to fulfill DNA pieces Section and the separation of edible oil, the edible oil after then separating is stored in bucket downwards by discharge pipe to be flowed into, when DNA fragmentation extracts After, staff closes suction pump and strong suction pump, then pulls active plugboard to the left, makes in active plugboard drive First strainer, silica gel filter membrane and second strainer in portion synchronously move to the left, at this time by aforesaid operations, so as to fulfill activity The taking-up of plate, facilitate staff on active plugboard DNA fragmentation carry out follow-up study detection, meanwhile, staff according to Experiment type needs, can be beforehand through opening weight display, the first weight sensor and the second weight sensor, i.e., and first Weight sensor carries out weight detecting to edible oil after storage bucket internal extraction, and the second weight sensor is to placing tube internal extraction Preceding edible oil weight detecting, the personnel that proceed with one's work know two kinds of gravimetric values by observing weight display, pass through two kinds at this time The contrast of gravimetric value, so as to facilitate staff to know the ratio of DNA fragmentation and edible oil, meets the needs of different experiments, In addition, to ensure the stabilization of edible oil extraction process with efficient, staff can open heater and temperature controller in advance, that is, heat Device is heated after opening, and the effect to be heated up by heater, realizes the rising of placement rack internal temperature, meanwhile, staff The temperature value of temperature controller is set, it is ensured that the heating-up temperature of heater is 50 ° -60 °, at this time by 50 ° -60 ° inside placement rack Temperature, so as to improve the reaction effect of 2%CTAB extraction buffers, edible oil DNA filters extraction element, and structure is skilful It is wonderful, it is powerful, first by micropore separating mechanism, it can realize the stop separating treatment to DNA fragmentation and oil molecule, secondly By negative suction system, persistently oil molecule can be aspirated, then promote the separation speed of DNA fragmentation and oil molecule Degree, eventually through above-mentioned, so that while realizing to DNA fragmentation high efficiency extraction in edible oil, also greatly alleviates work The operating burden of personnel, beneficial to the popularization and application of industry, meanwhile, when active plugboard Slideslip to the left, live because holder is in Dynamic plate lower position, i.e. holder can the remaining edible oil that is dripped in moving process of collection activity plate, avoid residual Remaining edible oil drips wantonly, so that the problem of polluting surrounding environment, cabinet is the side in order to place corresponding extracting tool and data Just staff operates with, and cover board is that staff opens trapped fuel chamber for convenience, that is, realizes the convenient of trapped fuel intracavitary portion Cleaning.

Brief description of the drawings

Fig. 1 is the stereogram of edible oil DNA filtering extraction elements；

Fig. 2 is the front view of edible oil DNA filtering extraction elements；

Fig. 3 is the sectional view of edible oil DNA filtering extraction elements；

Fig. 4 is silica gel filter membrane position cutaway view Amplified image；

Fig. 5 is the second weight sensor cutaway view Amplified image.

Housing 1, weight display 2, trapped fuel chamber 3, active plugboard 4, the first strainer 5, silica gel filter membrane 6, the second strainer 7, row Outlet pipe 8, strong suction pump 9, placing groove 10, storage bucket 11, the first weight sensor 12, placement rack 13, the second weight sensor 14th, temperature controller 15, heater 16, rotation lid 17, escape groove 18, placing tube 19, suction pump 20, suction pipe 21, connecting tube 22, receipts Receive cabinet 101, cover board 102, holder 103, protection network 1301, micropore 601.

Following embodiment will combine above-mentioned attached drawing and further illustrate.

Embodiment

Hereinafter, a variety of specific details are elaborated, in order to provide the saturating of the concept to forming described embodiment basis Thorough understanding, however, for the technical checking personnel of this area, it is clear that described embodiment can these be specific Put into practice in the case of some or all in details, in other cases, do not specifically describe well-known processing step Suddenly.

As shown in Figure 1, Figure 2, shown in Fig. 3, Fig. 4, Fig. 5, edible oil DNA filtering extraction elements, including housing 1, weight display 2nd, trapped fuel chamber 3, active plugboard 4, the first strainer 5, silica gel filter membrane 6, the second strainer 7, discharge pipe 8, strong suction pump 9, placing groove 10, Store bucket 11, the first weight sensor 12, placement rack 13, the second weight sensor 14, temperature controller 15, heater 16, rotation lid 17th, escape groove 18, placing tube 19, suction pump 20, suction pipe 21, connecting tube 22, the weight display 2 are located at outside housing 1 Wall upper end, the weight display 2 are connected with 1 screw thread of housing, and the trapped fuel chamber 3 is located at 1 inner upper end of housing, described Trapped fuel chamber 3 be rectangular cavities, the active plugboard 4 runs through 1 inside middle-end of housing, and the active plugboard 4 blocks with housing 1 Button is connected, and first strainer 5 is located at 4 inner upper end of active plugboard, first strainer 5 and 4 screw thread phase of active plugboard Even, the silica gel filter membrane 6 is located at 4 inside middle-end of active plugboard, and the silica gel filter membrane 6 is movably connected with active plugboard 4, institute The second strainer 7 stated is located at 4 interior lower end of active plugboard, and second strainer 7 is connected with 4 screw thread of active plugboard, described Discharge pipe 8 runs through 1 inside middle-end of housing, and the discharge pipe 8 is connected with 1 screw thread of housing, and strong suction pump 9 is positioned at discharge 8 middle-end of pipe, strong suction pump 9 are connected with 8 screw thread of discharge pipe, and strong suction pump 9 is connected with 1 screw thread of housing, institute The placing groove 10 stated is located on the downside of 1 interior forward end of housing, and the placing groove 10 is rectangular recess, and the storage bucket 11 is located at Inside placing groove 10, the storage bucket 11 is movably connected with housing 1, and first weight sensor 12 is located in housing 1 Bottom on the right side of portion, first weight sensor 12 are connected with 1 screw thread of housing, and first weight sensor 12 and again Amount 2 conducting wire of display is connected, and the placement rack 13 is located at 1 right side lower end of housing, the placement rack 13 and 1 screw thread phase of housing Even, second weight sensor 14 is located at 13 inside left bottom of placement rack, and second weight sensor 14 is with putting Put 13 screw thread of seat to be connected, and second weight sensor 14 is connected with 2 conducting wire of weight display, the temperature controller 15 On the right side of 13 outer wall upper end of placement rack, the temperature controller 15 is connected with 13 screw thread of placement rack, and the heater 16, which is located at, to be put 13 inner right side of seat is put, the heater 16 is connected with 13 screw thread of placement rack, and the heater 16 is led with temperature controller 15 Line is connected, and the rotation lid 17 is located at the top of placement rack 13, and the right side of rotation lid 17 is rotatedly connected with placement rack 13, The escape groove 18 is rectangular recess positioned at 17 inside left middle-end of lid, the escape groove 18 is rotated, the placing tube 19 are located at 13 inside left of placement rack, and the placing tube 19 is movably connected with placement rack 13, and the suction pump 20 is located at shell 1 right side upper end of body, the suction pump 20 are connected with 1 screw thread of housing, and the suction pipe 21 is located at 20 bottom of suction pump, described Suction pipe 21 be connected with 20 screw thread of suction pump, the connecting tube 22 runs through 1 inner right side upper end of housing, the connecting tube 22 one end are connected with 1 screw thread of housing, and 22 other end of connecting tube is connected with 20 screw thread of suction pump, in the housing 1 Cabinet 101 is additionally provided with the left of portion, the cabinet 101 is movably connected with housing 1, and 1 right hand top of housing is also set There is cover board 102, the cover board 102 is movably connected with housing 1, and 1 top left side of housing is additionally provided with holder 103, institute The holder 103 stated is movably connected with housing 1, and 13 inner right side of placement rack is additionally provided with protection network 1301, and described is anti- Protecting wire net 1301 is connected with 13 screw thread of placement rack, and a number of micropore 601 is also equipped with inside the silica gel filter membrane 6, described 601 a diameter of 2um-4um of micropore.

Edible oil DNA filters extraction element, and before extraction, staff is by weight display 2, the first weight sensor 12nd, the second weight sensor 14, strong suction pump 9, suction pump 20, temperature controller 15 and heater 16 and extraneous power lead phase Even, then open and rotate lid 17, then the placing tube 19 equipped with edible oil and 2%CTAB extraction buffers is inserted into placement rack 13 It is interior, and close and rotate lid 17, then by suction pipe 21 from escape groove 18 through insertion placing tube 19 inside, during extraction, work people Member is first turned on suction pump 20, i.e., the effect that suction is produced to suction pipe 21 is opened by suction pump 20, is realized in placing tube 19 Portion's edible oil is flowed into trapped fuel chamber 3 by suction pipe 21 by connecting tube 22, and then edible oil is wandered to the first strainer 5, because of The effect of one strainer 5, can tentatively filter edible oil, and then edible oil is wandered to silica gel filter membrane 6, because of silica gel filter membrane 6 Inside is distributed the micropore 601 of a number of diameter 2um-4um, i.e., by the effect of micropore 601, realizes to needed for edible oil inside The stop of DNA fragmentation is extracted, meanwhile, staff opens strong suction pump 9, i.e., negative by producing strength after 9 work of strong suction pump The effect of suction is pressed, the edible oil above silica gel filter membrane 6 is aspirated downwards, at this time by above-mentioned, inside quickening edible oil The speed wandered downwards by 6 and second strainer 7 of silica gel filter membrane less than the oil molecule of 601 diameter of micropore, meanwhile, more than micropore 601 diameter DNA fragmentations are blocked, eventually through above-mentioned, so as to fulfill the separation of DNA fragmentation and edible oil, after then separating Edible oil is stored downwards in bucket 11 by discharge pipe 8 and flowed into, and after DNA fragmentation extracts, staff closes suction pump 20 and strong suction pump 9, then active plugboard 4 is pulled to the left, make active plugboard 4 drive inside the first strainer 5, silica gel filter 6 and second strainer of film 7 is synchronous to be moved to the left, convenient so as to fulfill the taking-up of active plugboard 4 at this time by aforesaid operations Staff carries out DNA fragmentation on active plugboard 4 follow-up study detection, meanwhile, staff according to experiment type needs, Can be beforehand through weight display 2, the first weight sensor 12 and the second weight sensor 14 to be opened, i.e. the first weight senses Device 12 carries out weight detecting to edible oil after storage 11 internal extraction of bucket, and the second weight sensor 14 is to 19 internal extraction of placing tube Preceding edible oil weight detecting, the personnel that proceed with one's work know two kinds of gravimetric values by observing weight display 2, pass through two kinds at this time The contrast of gravimetric value, so as to facilitate staff to know the ratio of DNA fragmentation and edible oil, meets the needs of different experiments, In addition, to ensure the stabilization of edible oil extraction process with efficient, staff can open heater 16 and temperature controller 15 in advance, i.e., Heater 16 is heated after opening, and the effect to be heated up by heater 16, realizes the rising of 13 internal temperature of placement rack, together When, staff sets the temperature value of temperature controller 15, it is ensured that the heating-up temperature of heater 16 is 50 ° -60 °, passes through placement at this time The temperature of 50 ° -60 ° of the inside of seat 13, so as to improve the reaction effect of 2%CTAB extraction buffers, meanwhile, work as active plugboard 4 to the left Slideslip when, because holder 103 is in 4 lower position of active plugboard, i.e., holder 103 can collection activity plate 4 exist The remaining edible oil dripped in moving process, avoids remaining edible oil and drips wantonly, so that the problem of polluting surrounding environment, receives Cabinet 101 of receiving is to place corresponding extracting tool and data, facilitates operating with for staff, and cover board 102 is for convenience Staff opens trapped fuel chamber 3, that is, realizes the convenient cleaning inside trapped fuel chamber 3.

The utility model is not limited to above-mentioned specific embodiment, and the ordinary skill reviewer of this area is from above-mentioned structure Think of sets out, and without performing creative labour, a variety of conversion made, all fall within the scope of protection of the utility model.

Claims (6)

1. edible oil DNA filters extraction element, it is characterised in that including housing, weight display, trapped fuel chamber, active plugboard, the One strainer, silica gel filter membrane, the second strainer, discharge pipe, strong suction pump, placing groove, storage bucket, the first weight sensor, placement rack, Second weight sensor, temperature controller, heater, rotation lid, escape groove, placing tube, suction pump, suction pipe, connecting tube, it is described Weight display is located at housing exterior walls upper end, and the weight display is connected with housing screw thread, and the trapped fuel chamber is located at shell Body inner upper end, the trapped fuel chamber are rectangular cavities, and the active plugboard runs through enclosure interior middle-end, and the activity is inserted Plate is connected with housing buckle, and first strainer is located at active plugboard inner upper end, first strainer and active plugboard Screw thread is connected, and the silica gel filter membrane is located at middle-end inside active plugboard, and the silica gel filter membrane is movably connected with active plugboard, Second strainer is located at active plugboard interior lower end, and second strainer is connected with active plugboard screw thread, the row Outlet pipe runs through enclosure interior middle-end, and the discharge pipe is connected with housing screw thread, and strong suction pump is located at discharge pipe middle-end, The strong suction pump is connected with discharge pipe screw thread, and strong suction pump is connected with housing screw thread, the placing groove position On the downside of enclosure interior front end, the placing groove is rectangular recess, and the storage bucket position is inside placing groove, the receipts Bucket of receiving is movably connected with housing, and first weight sensor is located at bottom on the right side of enclosure interior, and first weight passes Sensor is connected with housing screw thread, and first weight sensor is connected with weight display conducting wire, the placement seat The lower end on the right side of housing, the placement rack are connected with housing screw thread, and second weight sensor is located inside placement rack Left side bottom, second weight sensor are connected with placement rack screw thread, and second weight sensor is shown with weight Showing that device conducting wire is connected, the temperature controller is located on the right side of placement rack outer wall upper end, and the temperature controller is connected with placement rack screw thread, The heater is located at placement rack inner right side, and the heater is connected with placement rack screw thread, and the heater with Temperature controller conducting wire is connected, and the rotation lid is located at the top of placement rack, is rotatedly connected on the right side of the rotation lid with placement rack, institute The escape groove stated is rectangular recess positioned at lid inside left middle-end, the escape groove is rotated, and the placing tube is positioned at placement Seat inside left, the placing tube are movably connected with placement rack, and the suction pump is located at upper end on the right side of housing, the pumping Sucking pump is connected with housing screw thread, and the suction pipe is located at suction pump bottom, and the suction pipe is connected with suction pump screw thread, institute The connecting tube stated is through upper end on the right side of enclosure interior, and described connecting tube one end is connected with housing screw thread, and the connecting tube The other end is connected with suction pump screw thread.

2. edible oil DNA as claimed in claim 1 filters extraction element, it is characterised in that is also set on the left of the enclosure interior There is cabinet, the cabinet is movably connected with housing.

3. edible oil DNA as claimed in claim 2 filters extraction element, it is characterised in that the housing right hand top is also set There is cover board, the cover board is movably connected with housing.

4. edible oil DNA as claimed in claim 3 filters extraction element, it is characterised in that is also set on the left of the case top There is holder, the holder is movably connected with housing.

5. edible oil DNA as claimed in claim 4 filters extraction element, it is characterised in that the placement rack inner right side is also Equipped with protection network, the protection network is connected with placement rack screw thread.

6. edible oil DNA as claimed in claim 5 filters extraction element, it is characterised in that also equal inside the silica gel filter membrane Equipped with a number of micropore, the micro-pore diameter is 2um-4um.