CN113502213A - Centrifugal cell separation can external shock carry out integrated cell extractor - Google Patents

Centrifugal cell separation can external shock carry out integrated cell extractor Download PDF

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CN113502213A
CN113502213A CN202110771738.8A CN202110771738A CN113502213A CN 113502213 A CN113502213 A CN 113502213A CN 202110771738 A CN202110771738 A CN 202110771738A CN 113502213 A CN113502213 A CN 113502213A
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shell
rod
block
cell
fixed
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杨芳
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/06Tubular
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B04CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04BCENTRIFUGES
    • B04B1/00Centrifuges with rotary bowls provided with solid jackets for separating predominantly liquid mixtures with or without solid particles
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B04CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04BCENTRIFUGES
    • B04B11/00Feeding, charging, or discharging bowls
    • B04B11/04Periodical feeding or discharging; Control arrangements therefor
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B04CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04BCENTRIFUGES
    • B04B9/00Drives specially designed for centrifuges; Arrangement or disposition of transmission gearing; Suspending or balancing rotary bowls
    • B04B9/02Electric motor drives
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/04Cell isolation or sorting

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
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  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Sustainable Development (AREA)
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  • Biomedical Technology (AREA)
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  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Clinical Laboratory Science (AREA)
  • Centrifugal Separators (AREA)

Abstract

The invention discloses a centrifugal cell separation integrated cell extractor capable of being externally vibrated, which comprises a shell, a feeding port, a motor, a fixed block and an electromagnet, wherein the feeding port is fixedly arranged on the top surface of the shell, a cell sap feeding port and a separation liquid feeding port are respectively and fixedly arranged at the tail end of the feeding port, and the inner surfaces of the cell sap feeding port and the separation liquid feeding port are penetrated through by a transmission gear. This centrifugal cell separation can external shock carry out integrated formula cell extractor, after will holding the material cup and place on placing the board, weight can suppress its gliding drive fixed fluted twist rod and extrude the lug, it is rotatory to let the lug drive drum, it slides and promotes the guide bar to drive the bracing piece through the connecting rod, let the baffler open and order about the liquid in the separator tube and fall into and hold the material cup, and the material cup weight that holds filled with also increases thereupon and drives the dead lever gliding totally and let the drum make a round of commentaries on classics reset, close the baffler, it gets the material to reach the ration.

Description

Centrifugal cell separation can external shock carry out integrated cell extractor
Technical Field
The invention relates to the technical field related to cell extraction, in particular to a centrifugal cell separation integrated cell extractor capable of being externally vibrated.
Background
Cells are basic units in biology, such as white blood cells and red blood cells, which constitute organisms in biology, and generally consist of cell walls, cell membranes, cytoplasm, organelles and cell nuclei, and when extracting cells, a cell extractor is used, by which the cells are extracted, but the cell extractor has some disadvantages:
one, for example, the application number and the name of the stem cell separation device are "CN 202010731801.0", by designing a separation cylinder, a piston is driven by a handle to move, and the separated stem cells are sucked and transferred into the separation cylinder, and the separation cylinder can be removed and transferred, so that the efficiency and quality of stem cell separation are improved, but the extraction can only be conveniently performed on the lowermost cells, the extraction amount is not controlled, and organelles with different sizes are easily sucked;
secondly, after the cells are extracted, the cells need to be put into clear liquid for storage or shake, so that the next test is facilitated, but the shaking needs to be carried out manually, and the extractor does not have the function of quickly connecting and shaking integrally, so that the extractor is inconvenient;
and thirdly, the material is not fed in proportion, so that separation liquid and cell sap can reasonably enter in proportion, rapid separation is conveniently carried out in the centrifugal process, and the operation is inconvenient.
Disclosure of Invention
The invention aims to provide a centrifugal cell separation and external oscillation integrated cell extractor, which solves the problem that the prior art does not have the functions of extracting according to quantity and effectively distinguishing organelles with different sizes; the device does not have the function of shaking by external oscillation; the function of liquid feeding in proportion is inconvenient to carry out.
In order to achieve the purpose, the invention provides the following technical scheme: a centrifugal cell separation integrated cell extractor capable of externally oscillating comprises a shell, a feeding port, a motor, a fixing block and an electromagnet, wherein the feeding port is fixedly arranged on the top surface of the shell, a cell liquid feeding port and a separation liquid feeding port are respectively and fixedly arranged at the tail end of the feeding port, the inner surfaces of the cell liquid feeding port and the separation liquid feeding port are penetrated through by a transmission gear, an inner groove is formed in the transmission gear, a push rod is connected to the outer surface of the transmission gear and penetrates through the outer surface of the shell, a fixing spring is fixedly connected to the tail end of the push rod, the tail end of the fixing spring is fixedly arranged in the shell, the motor is fixedly arranged on the surface of one side of the shell, a fixing column is installed at the output end of the motor, a first conical gear is fixedly connected to the tail end of the fixing column, and a transmission conical gear is connected to the upper surface of the first conical gear, the transmission bevel gear penetrates through the inner surface of the shell, the separation pipe is fixedly arranged on the inner surface of the transmission bevel gear, a driven bevel gear is connected to one side surface of the transmission bevel gear and is installed inside the shell, a support column is fixedly arranged on one side surface of the driven bevel gear, the first magnetic block is fixedly connected to the two ends of the tail end of the support column, a fixed block is fixedly arranged on one side surface of the shell, a cup placing groove is formed in one side surface of the shell, the tail end of the cup placing groove is fixedly connected with the inner surface of the shell, a support spring is fixedly arranged on the inner surface of the shell, a placing plate is fixedly connected to the top surface of the support spring, a fixed rod is fixedly connected to the bottom surface of the placing plate and penetrates through the inner surface of the shell, and a twist rod is fixedly arranged on the top surface of the tail end of the fixed rod, the twist rod runs through the internal surface of drum, and the internal fixed surface of drum is provided with the lug to the inside at the shell is installed to the drum, the top of drum is connected with the connecting rod, and the end-to-end connection of connecting rod has the bracing piece, and the end-to-end connection of bracing piece has the guide bar, the end-to-end connection of guide bar has the baffler, and the baffler runs through the internal surface of shell, the inside fixed electromagnet in bottom of shell is provided with, and the bottom surface of shell is run through by the increased face piece, one side fixed surface of increased face piece is connected with the ejector pad, and one side fixed surface of ejector pad is connected with third magnetic force piece, the surface of increased face piece is run through by the circular arc piece, and the fixed surface of circular arc piece is connected with and pulls the spring.
Preferably, the feeding ports are symmetrically distributed about a longitudinal center line of the shell, radii of the cell sap feeding port and the separation fluid feeding port on the feeding ports are different, and the shell and the push rod form a sliding structure.
Preferably, the transmission gear and the shell form a rotating structure, the transmission gear is meshed with the push rod, and the push rod and the shell form an elastic structure through a fixed spring.
Preferably, the fixed column and the housing form a rotating structure, the first bevel gear and the driven bevel gear on the fixed column are both in meshed connection with the transmission bevel gear, and the first bevel gear and the driven bevel gear are symmetrically distributed about the longitudinal center line of the transmission bevel gear respectively.
Preferably, the upper surface of fixed block is held the material cup and is run through, and holds the upper surface that the material cup runs through the bearing plate simultaneously to the bearing plate is located the inside of fixed block, one side fixed surface of bearing plate is connected with reset spring, and reset spring's end is fixed to be set up in the inside of fixed block, and one side fixed surface of bearing plate is connected with second magnetic block, and second magnetic block runs through the surface of fixed block and shell respectively simultaneously.
Preferably, hold material cup and bearing plate and be connected for the block, and bearing plate and fixed block constitution sliding construction to the fixed block passes through reset spring and bearing plate constitution elastic construction.
Preferably, the second magnetic block and the first magnetic block form a structure with opposite poles repelling each other, and the repulsion force between the second magnetic block and the first magnetic block is greater than the elastic acting force of the return spring.
Preferably, the placing plate and the shell form an elastic structure through a supporting spring, the placing plate and the fixing rod form a sliding structure with the shell, the twist rod on the fixing rod is connected with the protruding block in a clamping manner, and meanwhile, the cylinder on the protruding block and the shell form a rotating structure.
Preferably, the cylinder and the support rod form a rotating structure together with the connecting rod, the support rod and the blocking plate form a rotating structure together with the guide rod, and the upper surface of the blocking plate is attached to the lower surface of the separation tube.
Preferably, the third magnetic block and the electromagnet form a homopolar repulsion structure, the third magnetic block, the pushing block and the face increasing block form a sliding structure with the shell, the face increasing block and the arc block are circumferentially distributed about a central axis point of the overlooking face of the shell, the face increasing block and the arc block form a sliding structure, the arc block forms an elastic structure with the arc block through a pulling spring, and the face increasing block and the arc block are made of soft rubber materials.
Compared with the prior art, the invention has the beneficial effects that: this centrifugal cell separation can external shock carry out integrated formula cell extractor:
1. when the material storage cup is placed on the placing plate under the action of the supporting spring, the weight can press the material storage cup to slide downwards to drive the fixed twist rod to extrude the lug, the lug drives the cylinder to rotate slightly, the cylinder drives the supporting rod to slide through the connecting rod to push the guide rod, the blocking plate is opened to drive liquid in the separating pipe to fall into the material storage cup until the separating pipe is full, the weight of the full material storage cup is increased to drive the fixing rod to slide downwards, the lug is continuously extruded to the cylinder, the cylinder is reset by one turn, the blocking plate is closed, quantitative material taking is achieved, and particles with the bottom end completely settled and falling into the bottom of the pipe can be taken;
2. when the first conical gear drives the driven conical gear to rotate through the transmission conical gear, the driven conical gear can drive the first magnetic force block and the second magnetic force block to generate repulsion force, the second magnetic force block is driven by the bearing plate to slide in a reciprocating manner, and the clamped material storage cup is driven to shake, so that an external oscillation effect is achieved;
3. when pressing the push rod, the push rod can inwards slide, and when loosening the push rod, the push rod can pop out through fixed spring, thereby make the push rod reciprocating sliding, can mesh with drive gear when the push rod should slide, make drive gear rotatory reset again, reach the inside groove and no longer carry out the separation to cell liquid feed inlet and separation liquid feed inlet, then can make the different cell liquid feed inlet of radius and separation liquid feed inlet carry out the pan feeding, because both radiuses are different and the time of opening is the same, so the proportion of pan feeding equals, can effectually reach the function of proportion pan feeding.
Drawings
FIG. 1 is a schematic overall front cross-sectional structural view of the present invention;
FIG. 2 is a schematic side sectional view of the present invention;
FIG. 3 is a schematic top sectional view of the present invention;
FIG. 4 is a schematic view of the overall top view of the present invention;
FIG. 5 is a schematic view of a sectional view of the arc block and the surface increasing block;
FIG. 6 is a schematic view of the connection of the guide bar and the blocking plate in cross section;
FIG. 7 is a bottom sectional view of the connecting rod and cylinder assembly of the present invention.
In the figure: 1. a housing; 2. a feeding port; 3. a cell sap feed port; 4. a separation liquid feed inlet; 5. a transmission gear; 6. an inner tank; 7. a push rod; 8. fixing the spring; 9. a motor; 10. fixing a column; 11. a first bevel gear; 12. a transmission bevel gear; 13. a separation tube; 14. a support pillar; 15. a first magnetic block; 16. a fixed block; 17. a material storage cup; 18. a bearing plate; 19. a second magnetic block; 20. a return spring; 21. a cup placing groove; 22. a support spring; 23. placing the plate; 24. fixing the rod; 25. a twisted rod; 26. a cylinder; 27. a bump; 28. a connecting rod; 29. a support bar; 30. a guide bar; 31. a barrier plate; 32. an electromagnet; 33. a third magnetic block; 34. a push block; 35. adding flour blocks; 36. an arc block; 37. pulling the spring; 38. a driven bevel gear.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-7, the present invention provides a technical solution: a centrifugal cell separation integrated cell extractor capable of externally oscillating comprises a shell 1, a feed inlet 2, a cell sap feed inlet 3, a separation liquid feed inlet 4, a transmission gear 5, an inner groove 6, a push rod 7, a fixed spring 8, a motor 9, a fixed column 10, a first bevel gear 11, a transmission bevel gear 12, a separation tube 13, a support column 14, a first magnetic block 15, a fixed block 16, a material storage cup 17, a bearing plate 18, a second magnetic block 19, a return spring 20, a cup placing groove 21, a support spring 22, a placing plate 23, a fixed rod 24, a twist rod 25, a cylinder 26, a convex block 27, a connecting rod 28, a support rod 29, a guide rod 30, a baffle plate 31, an electromagnet 32, a third magnetic block 33, a push block 34, a face increasing block 35, an arc block 36, a pulling spring 37 and a driven bevel gear 38, wherein the feed inlet 2 is fixedly arranged on the top surface of the shell 1, and the cell sap feed inlet 3 and the separation liquid feed inlet 4 are fixedly arranged at the tail end of the feed inlet 2 respectively, and the inner surfaces of the cell sap feed port 3 and the separation liquid feed port 4 are penetrated by a transmission gear 5, an inner groove 6 is formed inside the transmission gear 5, the outer surface of the transmission gear 5 is connected with a push rod 7, the push rod 7 penetrates the outer surface of the shell 1, the tail end of the push rod 7 is fixedly connected with a fixed spring 8, the tail end of the fixed spring 8 is fixedly arranged inside the shell 1, one side surface of the shell 1 is fixedly provided with a motor 9, the output end of the motor 9 is provided with a fixed column 10, the tail end of the fixed column 10 is fixedly connected with a first conical gear 11, the upper surface of the first conical gear 11 is connected with a transmission conical gear 12, the transmission conical gear 12 penetrates the inner surface of the shell 1, the inner surface of the transmission conical gear 12 is fixedly provided with a separation tube 13, one side surface of the transmission conical gear 12 is connected with a driven conical gear 38, and the driven conical gear 38 is arranged inside the shell 1, a supporting column 14 is fixedly arranged on one side surface of the driven bevel gear 38, two ends of the tail end of the supporting column 14 are fixedly connected with a first magnetic block 15, a fixed block 16 is fixedly arranged on one side surface of the shell 1, a cup placing groove 21 is formed in one side surface of the shell 1, the tail end of the cup placing groove 21 is fixedly connected with the inner surface of the shell 1, a supporting spring 22 is fixedly arranged on the inner surface of the shell 1, a placing plate 23 is fixedly connected to the top surface of the supporting spring 22, a fixing rod 24 is fixedly connected to the bottom surface of the placing plate 23, the fixing rod 24 penetrates through the inner surface of the shell 1, a twist rod 25 is fixedly arranged on the top surface of the tail end of the fixing rod 24, the twist rod 25 penetrates through the inner surface of a cylinder 26, a bump 27 is fixedly arranged on the inner surface of the cylinder 26, the cylinder 26 is arranged inside the shell 1, a connecting rod 28 is connected to the top of the cylinder 26, and a supporting rod 29 is connected to the tail end of the connecting rod 28, and the end-to-end connection of bracing piece 29 has guide bar 30, the end-to-end connection of guide bar 30 has separation board 31, and separation board 31 runs through the internal surface of shell 1, the inside fixed electro-magnet 32 that is provided with in bottom of shell 1, and the bottom surface of shell 1 is run through by increased face piece 35, one side fixed surface of increased face piece 35 is connected with ejector pad 34, and one side fixed surface of ejector pad 34 is connected with third magnetic block 33, the surface of increased face piece 35 is run through by circular arc piece 36, and the fixed surface of circular arc piece 36 is connected with and pulls spring 37.
The feed inlet 2 is symmetrically distributed about the longitudinal central line of the shell 1, the radii of the cell sap feed inlet 3 and the separating liquid feed inlet 4 on the feed inlet 2 are different, the shell 1 and the push rod 7 form a sliding structure, and when the cell sap feed inlet 3 and the separating liquid feed inlet 4 have different radii, the feed amount is different in the same feed time.
The transmission gear 5 and the shell 1 form a rotating structure, the transmission gear 5 is meshed with the push rod 7, the push rod 7 and the shell 1 form an elastic structure through the fixed spring 8, and when the push rod 7 slides under the elastic acting force of the fixed spring 8, the push rod 7 is meshed with the transmission gear 5, and the transmission gear 5 rotates.
The fixed column 10 and the housing 1 form a rotating structure, the first bevel gear 11 and the driven bevel gear 38 on the fixed column 10 are in meshed connection with the transmission bevel gear 12, and the first bevel gear 11 and the driven bevel gear 38 are respectively and symmetrically distributed about a longitudinal center line of the transmission bevel gear 12, and when the fixed column 10 rotates, the fixed column 10 drives the first bevel gear 11 to rotate, so that the first bevel gear 11 is meshed with the transmission bevel gear 12, and the transmission bevel gear 12 rotates to be meshed with the driven bevel gear 38.
The upper surface of the fixed block 16 is penetrated by the material storage cup 17, the material storage cup 17 penetrates the upper surface of the bearing plate 18 at the same time, the bearing plate 18 is positioned inside the fixed block 16, one side surface of the bearing plate 18 is fixedly connected with the return spring 20, the tail end of the return spring 20 is fixedly arranged inside the fixed block 16, one side surface of the bearing plate 18 is fixedly connected with the second magnetic block 19, the material storage cup 17 is connected with the bearing plate 18 in a clamping manner, the bearing plate 18 and the fixed block 16 form a sliding structure, the fixed block 16 and the bearing plate 18 form an elastic structure through the return spring 20, the second magnetic block 19 penetrates the outer surfaces of the fixed block 16 and the shell 1 respectively, the second magnetic block 19 and the first magnetic block 15 form a heteropolar repulsion structure, the repulsion force of the second magnetic block 19 and the first magnetic block 15 is greater than the elastic acting force of the return spring 20, when the driven bevel gear 38 rotates, driven bevel gear 38 can drive support column 14 rotatory, make support column 14 drive first magnetic force piece 15 rotatory, make first magnetic force piece 15 intermittent type nature be close second magnetic force piece 19, make second magnetic force piece 19 receive promote bearing plate 18 slip under the reset spring 20 effect after the repulsion, under the spring effort, make bearing plate 18 shake, it vibrates to reach bearing plate 18 and drive the material cup 17 shock that holds of block, it holds material cup 17 and carries out the effect that the block reached the shock to reach the external installation of accessible.
Place board 23 and shell 1 through supporting spring 22 and constitute elastic construction, and place board 23 and dead lever 24 and all constitute sliding construction with shell 1, and twist pole 25 on the dead lever 24 is connected for the block with lug 27, and drum 26 on lug 27 constitutes rotating construction with shell 1 simultaneously, and when placing board 23 and receiving supporting spring 22's elastic force, place board 23 and can upwards slide for place board 23 drives twist pole 25 through dead lever 24 and slides, lets twist pole 25 slide and carry out the block with lug 27.
Drum 26 and bracing piece 29 all constitute revolution mechanic with connecting rod 28, and bracing piece 29 and baffler 31 all constitute revolution mechanic with guide bar 30, and the upper surface of baffler 31 laminates with the lower surface of separator 13 mutually, and when lug 27 received spiral helicine fried dough twist bar 25 extrusion, lug 27 can drive fixed connection's drum 26 rotatory, it is rotatory to make the one end that can drive connecting rod 28 when drum 26 is rotatory, let the other end of connecting rod 28 can drive bracing piece 29 and carry out reciprocating sliding, and when bracing piece 29 slides, bracing piece 29 can drive the one end of guide bar 30 and slide, let guide bar 30 drive baffler 31 and slide.
The third magnetic block 33 and the electromagnet 32 form a homopolar repulsion structure, the third magnetic block 33, the pushing block 34 and the face-increasing block 35 and the housing 1 form a sliding structure, the face-increasing block 35 and the arc block 36 are circumferentially distributed about a central axis point of a top view of the housing 1, the face-increasing block 35 and the arc block 36 form a sliding structure, the arc block 36 and the self form an elastic structure through a pulling spring 37, the face-increasing block 35 and the arc block 36 are both made of soft rubber materials, the electromagnet 32 is connected in parallel with the motor 9, when the electromagnet 32 is energized to generate repulsion to the third magnetic block 33, and the third magnetic block 33 receives the repulsion, the third magnetic block 33 pushes the pushing block 34, so that the pushing block 34 pushes the face-increasing block 35, because the face-increasing block 35 and the arc block 36 are made of soft rubber materials, the face-increasing block 35 can slide outwards and pull the arc block 36 to increase the area, so that the floor area is increased, and the shake generated in the centrifugal process is effectively reduced by the soft rubber material.
The working principle is as follows: when the centrifugal cell separation integrated cell extractor capable of externally oscillating is used, an external power supply is connected to the device, according to the figures 1, 2, 3 and 4, firstly, the push rod 7 is quickly pressed, the push rod 7 compresses the fixed spring 8 to be charged and slide to be meshed with the transmission gear 5, the transmission gear 5 is rotated, the cell sap feed port 3 and the separation liquid feed port 4 are opened through the inner groove 6, the feed inlet 2 enters liquid through the cell sap feed port 3 and the separation liquid feed port 4 and falls into the separation tube 13, when the push rod 7 is released, the push rod 7 is popped out through the charged fixed spring 8, the push rod 7 is re-meshed with the transmission gear 5, the transmission gear 5 is rotated and reset to re-separate the cell sap feed port 3 and the separation liquid feed port 4, and then the cell sap feed is proportionally fed through the cell sap feed port 3 and the separation liquid feed port 4 with different radiuses, at the moment, the baffle is pushed in to block the top of the separation tube 13, the motor 9 on the shell 1 is opened, the motor 9 drives the fixed column 10 to rotate rapidly, the first bevel gear 11 is driven to rotate to be meshed with the transmission bevel gear 12, the transmission bevel gear 12 drives the separation tube 13 to rotate rapidly, larger particles can be settled to the bottom of the tube due to centrifugal speed, and small particles can be suspended in supernatant at any time to achieve the separation effect, meanwhile, the electromagnet 32 is started simultaneously due to the starting of the motor 9, the electromagnet 32 generates repulsion force on the third magnetic block 33, the third magnetic block 33 pushes the push block 34, the push block 34 pushes the face-increasing block 35, the face-increasing block 35 occupies the floor area by pulling the circular arc block 36 under the action of the pulling spring 37, and the damping effect and the stability are improved by the face-increasing block 35 made of soft rubber materials;
after separation, according to fig. 1, fig. 2, fig. 3, fig. 4, fig. 5, fig. 6 and fig. 7, firstly, a material storage cup 17 is placed on a placing plate 23 through a cup placing groove 21, the placing plate 23 drives a fixing rod 24 downwards through a supporting spring 22, so that the fixing rod 24 drives a knurl rod 25 to extrude a bump 27, the bump 27 drives a cylinder 26 to rotate, the cylinder 26 drives a supporting rod 29 to slide slightly through a connecting rod 28, so that the supporting rod 29 pushes a blocking plate 31 through a guiding rod 30, the blocking plate 31 is driven to open, so that the liquid at the bottom end in the separation tube 13 falls into the material storage cup 17, when the weight of the material storage cup 17 is increased in the process of liquid loading, the placing plate 23 is driven to extrude the supporting spring 22 again to drive the fixing rod 24 to slide downwards, so that the knurl rod 25 also extrudes the bump 27, drives the cylinder 26 to rotate for one circle, the cylinder 26 drives the supporting rod 29 to reset and slide through the connecting rod 28, so that the support rod 29 pulls the separation plate 31 through the guide rod 30, the separation plate 31 separates the separation pipe 13 again to achieve quantitative discharging, simultaneously, all the materials at the bottom are taken out, then the material storage cups 17 filled with the cells can be taken out and clamped on the bearing plate 18, the other material storage cup 17 is placed on the placing plate 23 to take out and circulate the liquid not filled with the cells, when the transmission bevel gear 12 rotates, the transmission bevel gear 12 is meshed with the driven bevel gear 38, so that the driven bevel gear 38 drives the first magnetic block 15 on the supporting column 14 to rotate and intermittently generate repulsive force to the second magnetic block 19, make second magnetic force piece 19 promote bearing plate 18 under the effect of reset spring 20, let bearing plate 18 drive in fixed block 16 and hold material cup 17 and vibrate, reach the effect that can external installation hold material cup 17 and vibrate, increased holistic practicality.
Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that various changes, modifications, equivalents, and improvements may be made without departing from the spirit and scope of the invention.

Claims (10)

1. The utility model provides a but centrifugal cell separation external shock carries out integrated cell extraction machine, includes shell (1), pan feeding mouth (2), motor (9), fixed block (16) and electro-magnet (32), its characterized in that: the cell liquid separation device is characterized in that a feeding port (2) is fixedly arranged on the top surface of the shell (1), the tail end of the feeding port (2) is respectively and fixedly provided with a cell liquid feeding port (3) and a separation liquid feeding port (4), the inner surfaces of the cell liquid feeding port (3) and the separation liquid feeding port (4) are penetrated through by a transmission gear (5), an inner groove (6) is formed in the transmission gear (5), the outer surface of the transmission gear (5) is connected with a push rod (7), the push rod (7) penetrates through the outer surface of the shell (1), the tail end of the push rod (7) is fixedly connected with a fixed spring (8), the tail end of the fixed spring (8) is fixedly arranged in the shell (1), a motor (9) is fixedly arranged on the surface of one side of the shell (1), a fixed column (10) is arranged at the output end of the motor (9), and a first conical gear (11) is fixedly connected with the tail end of the fixed column (10), the upper surface of the first conical gear (11) is connected with a transmission conical gear (12), the transmission conical gear (12) penetrates through the inner surface of the shell (1), a separation pipe (13) is fixedly arranged on the inner surface of the transmission conical gear (12), a driven conical gear (38) is connected to the surface of one side of the transmission conical gear (12), the driven conical gear (38) is installed inside the shell (1), a supporting column (14) is fixedly arranged on the surface of one side of the driven conical gear (38), two ends of the tail end of the supporting column (14) are fixedly connected with a first magnetic block (15), a fixing block (16) is fixedly arranged on the surface of one side of the shell (1), a cup placing groove (21) is formed in the surface of one side of the shell (1), the inner surface of the shell (1) is fixedly connected with the inner surface of the shell (1), and a supporting spring (22) is fixedly arranged on the inner surface of the shell (1), a placing plate (23) is fixedly connected to the top surface of the supporting spring (22), a fixing rod (24) is fixedly connected to the bottom surface of the placing plate (23), the fixing rod (24) penetrates through the inner surface of the shell (1), a twist rod (25) is fixedly arranged on the top surface of the tail end of the fixing rod (24), the twist rod (25) penetrates through the inner surface of the cylinder (26), a convex block (27) is fixedly arranged on the inner surface of the cylinder (26), the cylinder (26) is installed inside the shell (1), a connecting rod (28) is connected to the top of the cylinder (26), a supporting rod (29) is connected to the tail end of the connecting rod (28), a guide rod (30) is connected to the tail end of the supporting rod (29), a blocking plate (31) is connected to the tail end of the guide rod (30), the blocking plate (31) penetrates through the inner surface of the shell (1), and an electromagnet (32) is fixedly arranged inside the bottom end of the shell (1), and the bottom surface of shell (1) is run through by increasing a piece (35), increase one side fixed surface of a piece (35) and be connected with ejector pad (34), and one side fixed surface of ejector pad (34) is connected with third magnetic block (33), the surface that increases a piece (35) is run through by arc piece (36), and the fixed surface of arc piece (36) is connected with and pulls spring (37).
2. The centrifugal cell separation integrated cell extractor as claimed in claim 1, wherein: the feed inlet (2) is symmetrically distributed about the longitudinal central line of the shell (1), the radii of the cell sap feed inlet (3) and the separating liquid feed inlet (4) on the feed inlet (2) are different, and the shell (1) and the push rod (7) form a sliding structure.
3. The centrifugal cell separation integrated cell extractor as claimed in claim 1, wherein: the transmission gear (5) and the shell (1) form a rotating structure, the transmission gear (5) is meshed with the push rod (7), and the push rod (7) and the shell (1) form an elastic structure through a fixed spring (8).
4. The centrifugal cell separation integrated cell extractor as claimed in claim 1, wherein: the fixed column (10) and the shell (1) form a rotating structure, a first bevel gear (11) and a driven bevel gear (38) on the fixed column (10) are in meshed connection with the transmission bevel gear (12), and the first bevel gear (11) and the driven bevel gear (38) are symmetrically distributed about the longitudinal center line of the transmission bevel gear (12) respectively.
5. The centrifugal cell separation integrated cell extractor as claimed in claim 1, wherein: the upper surface of fixed block (16) is run through by holding material cup (17), and holds material cup (17) and run through the upper surface of bearing plate (18) simultaneously to bearing plate (18) are located the inside of fixed block (16), one side fixed surface of bearing plate (18) is connected with reset spring (20), and reset spring (20) terminal fixed the setting in the inside of fixed block (16), and one side fixed surface of bearing plate (18) is connected with second magnetic block (19), and second magnetic block (19) run through the surface of fixed block (16) and shell (1) respectively simultaneously.
6. The centrifugal cell separation integrated cell extractor as claimed in claim 5, wherein: hold material cup (17) and bearing plate (18) and be connected for the block, and bearing plate (18) and fixed block (16) constitute sliding construction to fixed block (16) pass through reset spring (20) and bearing plate (18) constitution elastic construction.
7. The centrifugal cell separation integrated cell extractor as claimed in claim 6, wherein: the second magnetic block (19) and the first magnetic block (15) form a structure with opposite poles repelling each other, and the repulsion force of the second magnetic block (19) and the first magnetic block (15) is larger than the elastic acting force of the reset spring (20).
8. The centrifugal cell separation integrated cell extractor as claimed in claim 1, wherein: the placing plate (23) and the shell (1) form an elastic structure through the supporting spring (22), the placing plate (23) and the fixing rod (24) form a sliding structure with the shell (1), the twist rod (25) on the fixing rod (24) is connected with the bump (27) in a clamping mode, and meanwhile the cylinder (26) on the bump (27) and the shell (1) form a rotating structure.
9. The centrifugal cell separation integrated cell extractor as claimed in claim 8, wherein: the cylinder (26) and the support rod (29) form a rotating structure with the connecting rod (28), the support rod (29) and the blocking plate (31) form a rotating structure with the guide rod (30), and the upper surface of the blocking plate (31) is attached to the lower surface of the separation pipe (13).
10. The centrifugal cell separation integrated cell extractor as claimed in claim 1, wherein: third magnetic block (33) and electro-magnet (32) constitute homopolar repulsion structure, and third magnetic block (33), ejector pad (34) and increase a face piece (35) and all constitute sliding construction with shell (1) to increase a face piece (35) and circular arc piece (36) and all be the circumference distribution about overlooking a face central axis point of shell (1), increase a face piece (35) and circular arc piece (36) and constitute sliding construction, and circular arc piece (36) constitute elastic construction with self through dragging spring (37), and increase a face piece (35) and circular arc piece (36) and be the preparation of flexible glue material and form.
CN202110771738.8A 2021-07-08 2021-07-08 Centrifugal cell separation can external shock carry out integrated cell extractor Withdrawn CN113502213A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202110771738.8A CN113502213A (en) 2021-07-08 2021-07-08 Centrifugal cell separation can external shock carry out integrated cell extractor

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202110771738.8A CN113502213A (en) 2021-07-08 2021-07-08 Centrifugal cell separation can external shock carry out integrated cell extractor

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CN113502213A true CN113502213A (en) 2021-10-15

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114733654A (en) * 2022-06-10 2022-07-12 胜利油田新邦石油科技有限责任公司 Curing centrifugal device for preparing polyaluminium chloride medicament composition

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114733654A (en) * 2022-06-10 2022-07-12 胜利油田新邦石油科技有限责任公司 Curing centrifugal device for preparing polyaluminium chloride medicament composition
CN114733654B (en) * 2022-06-10 2022-08-26 胜利油田新邦石油科技有限责任公司 Curing centrifugal device for preparing polyaluminium chloride medicament composition

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Application publication date: 20211015