CN207284947U - A kind of programmed cell cooling box - Google Patents
A kind of programmed cell cooling box Download PDFInfo
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- CN207284947U CN207284947U CN201721188367.6U CN201721188367U CN207284947U CN 207284947 U CN207284947 U CN 207284947U CN 201721188367 U CN201721188367 U CN 201721188367U CN 207284947 U CN207284947 U CN 207284947U
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Abstract
The utility model discloses a kind of programmed cell cooling box, include：Box body, is provided with some sample slots for being used to place cryopreservation tube, isopropanol is housed in the box body in it；Box lid, lid are located in the opening of the cassette upper end；Sample rack, is detachably arranged in the box body and above the sample slot；Some jacks are provided with the sample rack, and the jack is corresponded with the sample slot；The cryopreservation tube is plugged in the jack of sample rack, and the sample equipped with cryopreservation tube is placed in the box body, and cryopreservation tube lower end is injected in the sample slot.Programmed cell cooling box provided by the utility model, the setting of sample rack is added on the basis of existing structure, cryopreservation tube is first attached on sample rack, then place into the box body of programmed cell cooling box, sample rack is transferred directly in freezing storing box after program cools down, so as to realize the loading together of multiple cryopreservation tubes and taking-up, the time that liquid nitrogen container is transferred to from program temperature reduction box is saved, reduce temperature change irreversible damage caused by freeze-stored cell, it is simple in structure, it is easy to operate, it is efficient.
Description
Technical field
It the utility model is related to technical field of cell biology, and in particular to a kind of programmed cell cooling box.
Background technology
The long-term storage of cell is generally all placed in liquid nitrogen container., it is necessary to which slow cooling, avoids thin during cell cryopreservation
Intracellular moisture quickly forms ice crystal and is damaged caused by cell.The process of current procedure cooling can pass through programmed cooling instrument or journey
What sequence cooling box was realized.Programmed cooling instrument is expensive, is generally all completed by program temperature reduction box.Freeze-stored cell is placed on cryopreservation tube
In, the program temperature reduction box for adding good isopropanol is then placed in, -80 DEG C of ultra low temperature freezers is placed on and stays overnight, be transferred to liquid nitrogen within second day
In tank.Existing program temperature reduction box may only each place 18 cryopreservation tubes.
Stem cell, which establishes master library and work storehouse, disposably to be needed to freeze hundreds of cells, it is necessary to tens programs be cooled down
Box, it is cumbersome.Cell is from during -80 DEG C of ultra low temperature freezers are transferred to liquid nitrogen container, heavy workload, time length, room temperature behaviour
The cell for making to make to freeze heats up and irreversible damage is caused to cell.In addition, the process of transfer cell, which exists, misplaces position
May.The above problem is urgently to be resolved hurrily.
Utility model content
The problem of for being proposed in background technology, the utility model provide a kind of programmed cell cooling box, include：
Box body, is provided with a sample slot structure in it, is equipped with the space between the sample slot structure and the box body
Isopropanol；Some sample slots for being used to place cryopreservation tube are provided with the sample slot structure, the sample slot lower end puts in institute
State in isopropanol；
Box lid, lid are located in the opening of the cassette upper end；
Sample rack, is detachably arranged in the box body and is located at the sample slot superstructure；On the sample rack
Some jacks are provided with, and the jack is corresponded with the sample slot；
The cryopreservation tube is plugged in the jack of sample rack, and the sample equipped with cryopreservation tube is placed in the box body, and is made
Cryopreservation tube lower end is obtained to inject in the sample slot.
It is preferred that the sample slot structure is detachably arranged in the box body.
It is preferred that the aperture of the sample slot freezes pipe diameter more than described.
It is preferred that lug boss is provided with the sample rack upper surface, for pulling out the sample rack.
It is preferred that can realize the fast transfer of cryopreservation tube between freezing storing box, the box body, sample rack and freezing storing box
Shape matches, and can be placed directly into by the sample rack with cryopreservation tube taken out in box body in freezing storing box.
It is preferred that the box body, box lid, sample rack are made of low-temperature resistance material.
It is preferred that the side of the box lid is hinged with the box body, opposite side is buckled in the box body by buckle structure
Together.
It is preferred that surrounding is provided with spongy layer on the outside of the sample slot.
The utility model is due to using above technical scheme, being allowed to compared with prior art, have the following advantages that and accumulate
Pole effect：
Programmed cell cooling box provided by the utility model, adds the setting of sample rack on the basis of existing structure,
Cryopreservation tube is first attached on sample rack, is then placed into the box body of programmed cell cooling box, so as to realize multiple cryopreservation tubes
Loading together and taking-up, it is simple in structure, it is easy to operate；
Meanwhile the programmed cell cooling box is matched with freezing storing box, the sample rack equipped with some cryopreservation tubes is from cell journey
It after integrally being taken out in sequence cooling box, can be directly loaded into corresponding freezing storing box, realize multiple cryopreservation tubes and quickly turn at the same time
Move, it is easy to operate, efficient, not error-prone；Cell caused by heating up when avoiding shifting one by one for a long time because of the cell that freezes
The problem of irreversible damage；On this basis, we can greatly increase is used to house cryopreservation tube in programmed cell cooling box
The quantity of sample slot.
Brief description of the drawings
With reference to attached drawing, pass through the above-mentioned and other spy for stating detailed description, being more clearly understood that the utility model hereafter
Seek peace advantage, wherein：
Fig. 1 is the structure diagram of programmed cell cooling box provided by the utility model；
Fig. 2 is the sectional view of programmed cell cooling box provided by the utility model；
Fig. 3 is the structure diagram of sample rack in the utility model.
Symbol description：
1- box bodies
102- gaps
2- box lids
3- sample racks
301- jacks
4- cryopreservation tubes
5- lug bosses
Embodiment
Referring to the attached drawing for showing the utility model embodiment, the utility model is described in more detail.However, this reality
It can in many different forms be realized with new, and should not be construed as being limited by the embodiment herein proposed.On the contrary, propose
These embodiments be in order to reach fully and complete disclosure, and make those skilled in the art understand completely this practicality newly
The scope of type.In these attached drawings, for clarity, the size and relative size in layer and region may be exaggerated.
With reference to Fig. 1-3, the utility model provides a kind of programmed cell cooling box, includes box body 1, box lid 2, sample slot
Structure 6 and sample rack 3；Sample slot structure 6 is arranged in box body 1, and is equipped with the space between sample slot structure 6 and box body 1
Isopropanol；Some sample slots 601 for being used to place cryopreservation tube are provided with sample slot structure 6,601 lower end of sample slot puts in isopropyl
In alcohol；The lid of box lid 2 is located in the opening of 1 upper end of box body；Sample rack 3 is detachably arranged in box body 1 and is located at sample slot knot
Above structure 6, some jacks 301 are provided with sample rack 3, and jack 301 is corresponded with sample slot 601；Cryopreservation tube 4 is plugged in
In the jack 301 of sample rack 3, the sample rack 3 equipped with cryopreservation tube 4 is put into box body 2, and causes 4 lower end of cryopreservation tube to inject sample
In groove 601.
Wherein, the effect of isopropanol is to ensure freeze-stored cell with -1 DEG C/min after program temperature reduction box is put into -80 DEG C of refrigerators
Speed slow cooling, the too fast intracellular ice crystal that produces that avoids cooling down causes to damage to cell.
Programmed cell cooling box provided by the utility model, adds the setting of sample rack on the basis of existing structure,
Cryopreservation tube is first attached on sample rack, is then placed into the box body of programmed cell cooling box, so as to realize multiple cryopreservation tubes
Loading together and taking-up, it is simple in structure, it is easy to operate.
Meanwhile the programmed cell cooling box is matched with freezing storing box, the sample rack equipped with some cryopreservation tubes is from cell journey
It after integrally being taken out in sequence cooling box, can be directly loaded into corresponding freezing storing box, realize multiple cryopreservation tubes and quickly turn at the same time
Move, it is easy to operate, efficient, not error-prone；Cell caused by heating up when avoiding shifting one by one for a long time because of the cell that freezes
The problem of irreversible damage；On this basis, we can greatly increase is used to house cryopreservation tube in programmed cell cooling box
The quantity of sample slot.
In the present embodiment, the shape of box body 1, sample rack 3 and freezing storing box matches, so that by being taken in box body 1
The sample rack equipped with cryopreservation tube 4 gone out can be placed directly into freezing storing box.
Illustrate so that freezing storing box is in square shape, is disposed with 10 × 10 thereon and is used for the jacks for accommodating cryopreservation tube as an example, it is corresponding
, in the present embodiment, box body 1, sample rack 3 are in the rectangle to match, are disposed with box body with 10 × 10 sample slots
601 sample slot structure 6, is correspondingly arranged on 10 × 10 jack on sample rack 3
Certainly, in other embodiments, box body 1 and sample rack 3 also can rounded, polygon etc., sample slot thereon
601st, jack 301 may be designed in 5 × 5 etc., can as the case may be adjusted, not be limited herein.
In the present embodiment, the size of the jack on sample rack 3 and the outside diameter of cryopreservation tube 4 mutually coincide, or jack 301
Surrounding material can be necessarily elastic, so that the body of cryopreservation tube 4 is passed through and is fixed in jack 301, pacifies so as to fulfill cryopreservation tube 4
It is attached on sample rack, some cryopreservation tubes 4 can be also removed together when removing sample rack；Sample slot in box body 1 it is straight
Footpath is more than the body of cryopreservation tube 4, so that cryopreservation tube can be successfully inserted in sample slot.
Further, lug boss 5 is provided with 3 upper surface of sample rack, for pulling out sample rack 3.Wherein, lug boss 5
Shape can be a column (as shown in figures 2-3), or hook-shaped etc., not be limited herein.
In the present embodiment, as shown in Figure 2, formed with some sample slots for extending downwardly setting in sample slot structure 6
601, and the notch of sample slot 601 is upward, the internal diameter of sample slot 601 is slightly larger than the outside diameter of cryopreservation tube 4, more than 4 sample of cryopreservation tube made
Free gap is left between this groove 601, in order to being inserted and removed from for cryopreservation tube 4.Sample slot structure 6 is detachably arranged in box
In body 1, consequently facilitating in installation process, sample slot structure 6 is first taken out, then isopropanol is added into box body 1, finally again by sample
This slot structure shape is put into box body 1 so that 601 outside lower end of sample slot is put in isopropanol.
Further, 601 outside surrounding of sample slot is provided with spongy layer；
In the present embodiment, the side of box lid 2 is hinged with box body, opposite side and box body 1 are buckled together by buckle structure.
Certainly, in other embodiments, when box lid 2, box body 1 are rounded, box lid 2 can also be twisted by helicitic texture and be set to box body
On 1, it is not limited herein.
In the present embodiment, box body 1, box lid 2, sample rack 3, sample slot structure 6 are made of low-temperature resistance material, are prevented
It is damaged by frost under low temperature environment.
Below with regard to the operation principle of programmed cell cooling box provided by the utility model, it is further described：
First sample slot structure 6 is opened, isopropanol is added in box to graduation mark；Then sample slot structure 6 is put into box
In body 1；
Some cryopreservation tubes equipped with freeze-stored cell are encased in the jack on sample rack 3, then sample rack 3 is put into box
In vivo, and each cryopreservation tube is inserted into corresponding sample slot 601；
Box lid 2 is covered, whole programmed cell cooling box is placed into -80 DEG C of ultra low temperature freezer coolings overnight；
After cooling, the sample rack of related cryopreservation tube is taken out, is entirely moved into corresponding freezing storing box, will finally be frozen again
Deposit box and be transferred to liquid nitrogen container stored for extended periods.
Those skilled in the art should be understood that the utility model can be realized without de- with many other concrete forms
From the spirit or scope of itself.Although have been described for the case study on implementation of the utility model, it should be understood that the utility model should not limit
Be made as these embodiments, those skilled in the art can as defined by the following claims the spirit of the utility model and
Within the scope of make and changing and modifications.
Claims (8)
1. a kind of programmed cell cooling box, it is characterised in that include：
Box body, is provided with a sample slot structure in it, isopropyl is housed in the space between the sample slot structure and the box body
Alcohol；Some sample slots for being used to place cryopreservation tube are provided with the sample slot structure, the sample slot lower end puts in described different
In propyl alcohol；
Box lid, lid are located in the opening of the cassette upper end；
Sample rack, is detachably arranged in the box body and is located at the sample slot superstructure；Set on the sample rack
There are some jacks, and the jack is corresponded with the sample slot；
The cryopreservation tube is plugged in the jack of sample rack, and the sample equipped with cryopreservation tube is placed in the box body, and to freeze
Pipe lower end is deposited to inject in the sample slot.
2. programmed cell cooling box according to claim 1, it is characterised in that the sample slot structure is removably installed
In the box body.
3. programmed cell cooling box according to claim 1, it is characterised in that the aperture of the sample slot is more than the jelly
Deposit pipe diameter.
4. programmed cell cooling box according to claim 1, it is characterised in that be provided with the sample rack upper surface convex
The portion of rising, for pulling out the sample rack.
5. programmed cell cooling box according to claim 1, it is characterised in that cryopreservation tube can be realized between freezing storing box
Fast transfer, the shape of the box body, sample rack and freezing storing box match, by the sample with cryopreservation tube taken out in box body
Frame can be placed directly into freezing storing box.
6. programmed cell cooling box according to claim 1, it is characterised in that the box body, box lid, sample rack are using anti-
Cryogenic material is made.
7. programmed cell cooling box according to claim 1, it is characterised in that cut with scissors with the box body side of the box lid
Connect, opposite side is buckled together with the box body by buckle structure.
8. programmed cell cooling box according to claim 1, it is characterised in that surrounding is provided with sea on the outside of the sample slot
Continuous layer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721188367.6U CN207284947U (en) | 2017-09-15 | 2017-09-15 | A kind of programmed cell cooling box |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721188367.6U CN207284947U (en) | 2017-09-15 | 2017-09-15 | A kind of programmed cell cooling box |
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| Publication Number | Publication Date |
|---|---|
| CN207284947U true CN207284947U (en) | 2018-05-01 |
Family
ID=62442745
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721188367.6U Active CN207284947U (en) | 2017-09-15 | 2017-09-15 | A kind of programmed cell cooling box |
Country Status (1)
| Country | Link |
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| CN (1) | CN207284947U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109164845A (en) * | 2018-09-11 | 2019-01-08 | 希瑞干细胞科技有限公司 | Band can write the programmed cooling instrument of cooling process |
| CN109997838A (en) * | 2019-03-27 | 2019-07-12 | 安徽环球基因科技有限公司 | A kind of practical cell cryopreservation cooling box |
-
2017
- 2017-09-15 CN CN201721188367.6U patent/CN207284947U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109164845A (en) * | 2018-09-11 | 2019-01-08 | 希瑞干细胞科技有限公司 | Band can write the programmed cooling instrument of cooling process |
| CN109997838A (en) * | 2019-03-27 | 2019-07-12 | 安徽环球基因科技有限公司 | A kind of practical cell cryopreservation cooling box |
| CN109997838B (en) * | 2019-03-27 | 2021-10-08 | 安徽环球基因科技有限公司 | Practical cell cryopreserving cooling box |
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