CN207159236U - A kind of cell culture apparatus for strengthening oxygen supply - Google Patents
A kind of cell culture apparatus for strengthening oxygen supply Download PDFInfo
- Publication number
- CN207159236U CN207159236U CN201720905423.7U CN201720905423U CN207159236U CN 207159236 U CN207159236 U CN 207159236U CN 201720905423 U CN201720905423 U CN 201720905423U CN 207159236 U CN207159236 U CN 207159236U
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- China
- Prior art keywords
- oxygen
- incubator
- plc
- sensor
- gas cylinder
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Abstract
The utility model discloses a kind of cell culture apparatus for strengthening oxygen supply,It is related to biological cell technical field,Including incubator and PLC,Fixed oxygen gas bottle is separated in the internal side wall of the incubator,Nitrogen gas cylinder,Dioxide bottle and tolerance adjustment control device,The oxygen gas cylinder,The nitrogen gas cylinder and the dioxide bottle are connected by tracheae with the tolerance adjustment control device,The oxygen gas cylinder is passed through by tracheae and oxygen air flow controller in the nutrient solution of the culture dish one,Gas concentration lwevel sensor and oxygen concentration sensor are fixed in the internal side wall of the incubator,The PLC is fixed in the exterior side wall of the incubator,The utility model can not only meet the environment needed for general cell culture,The supply of oxygen cell can also especially be strengthened,It is greatly convenient to be provided for researcher's research aerobic cell.
Description
Technical field
Biological cell technical field is the utility model is related to, more particularly to a kind of cell culture apparatus for strengthening oxygen supply.
Background technology
Cell culture technology is also in cell culture.No matter for whole biotechnology, or the biology of one of them
For clone technology, cell culture is all an essential process, the extensive clone of cell culture inherently cell.
Cell culture technology can turn into many cells that are simple unicellular or seldom breaking up by a cell by mass propgation, and this is
The essential link of clone technology, and the clone of cell culture inherently cell.Obtained largely by cell culture
Cell or its metabolite.Because biological product is got from cell, it is possible to which it is biotechnology to say cell culture technology
In most crucial, most basic technology.
In scientific research, different cells generally requires different culture environments, oxygen content, carbon dioxide in air
All cell growths such as amount, temperature, humidity and culture matrix have opposite impacts on, and aerobic cell then have to it is constant
Oxygen could quickly breed；Because cell is usually bred in the medium, but the dissolved oxygen in culture medium and and air
Middle oxygen content is different, so just needing one kind can simultaneously to monitor oxygen content in air and culture medium, and can is timely
Device to cell enhancing oxygen supply.
Utility model content
The utility model embodiment provides a kind of cell culture apparatus for strengthening oxygen supply, to solve in the prior art
The problem of existing.
A kind of cell culture apparatus for strengthening oxygen supply, including incubator and PLC, the incubator inner bottom surface
Fixed pole, pole top mounting rotary electric machine, fixed underpan in the rotating shaft of the electric rotating machine are fixed on the chassis
Culture dish one, fixed oxygen gas bottle, nitrogen gas cylinder, dioxide bottle and tolerance are separated in the internal side wall of the incubator and is adjusted
Controller is saved, the oxygen gas cylinder, the nitrogen gas cylinder and the dioxide bottle are adjusted by tracheae and the tolerance
Controller connects, and the oxygen gas cylinder connects oxygen air flow controller by tracheae, and the oxygen air flow controller passes through tracheae
It is passed through in the nutrient solution of the culture dish one, the incubator inner tip installation temperature controller and bulb, the incubator
Internal side wall on fix gas concentration lwevel sensor and oxygen concentration sensor, it is fixed in the exterior side wall of the incubator
The PLC, the PLC are electrically connected with the gas concentration lwevel sensor and the oxygen concentration sensor
Connect, dissolved oxygen sensor one and acdity and basicity measuring meter are set in the culture dish one, the incubator inner bottom surface fixes culture
Ware two, sets dissolved oxygen sensor two in the culture dish two, the PLC and the dissolved oxygen sensor one, described
Acdity and basicity measuring meter and the dissolved oxygen sensor two are electrical connection, the PLC and the oxygen air flow controller,
Tolerance adjustment control device, the temperature controller, the bulb and the electric rotating machine are electrical connection.
It is preferred that inflation inlet is respectively provided with the oxygen gas cylinder, the nitrogen gas cylinder and the dioxide bottle, it is described
Gas outlet is set on tolerance adjustment control device.
It is preferred that the incubator inner bottom surface fixes the culture dish two, the PLC is 51 single-chip microcomputers.
The utility model advantage：The utility model can not only meet the environment needed for general cell culture, additionally it is possible to special
Not Zeng Qiang oxygen cell supply, for researcher study aerobic cell provide it is greatly convenient.
Brief description of the drawings
Fig. 1 is a kind of structural representation of the cell culture apparatus for enhancing oxygen supply that the utility model embodiment provides.
Description of reference numerals：
1- incubators, 2- oxygen gas cylinders, 3- oxygen air flow controllers, 4- tolerance adjustment control devices, 5- nitrogen gas cylinders, 6- temperature
Spend controller, 7- bulbs, 8- dioxide bottles, 9-PLC controllers, 10- gas concentration lwevel sensors, 11- oxygen concentrations
Sensor, 12- dissolved oxygen sensors one, 13- culture dishes one, 14- chassis, 15- electric rotating machines, 16- poles, 17- dissolved oxygens pass
Sensor two, 18- culture dishes two, 19- acdity and basicity measuring meters.
Embodiment
With reference to the accompanying drawing in new embodiment, the technical scheme in the embodiment of the utility model is carried out clear, complete
Whole description, it is to be understood that the scope of protection of the utility model is not limited by embodiment.
Reference picture 1, the utility model provide a kind of cell culture apparatus for strengthening oxygen supply, including incubator 1 and PLC
Controller 9, the inner bottom surface of incubator 1 fix pole 16, the top mounting rotary electric machine 15 of pole 16, the electric rotating
Fixed underpan 14 in the rotating shaft of machine 15, culture dish 1 is fixed on the chassis 14, is separated in the internal side wall of the incubator 1
Fixed oxygen gas bottle 2, nitrogen gas cylinder 5, dioxide bottle 8 and tolerance adjustment control device 4, the oxygen gas cylinder 2, the nitrogen
Gas cylinder 5 and the dioxide bottle 8 are connected by tracheae with the tolerance adjustment control device 4, and the oxygen gas cylinder 2 passes through
Tracheae connection oxygen air flow controller 3, the oxygen air flow controller 3 is passed through the nutrient solution of the culture dish 1 by tracheae
In, the inner tip of incubator 1 installs temperature controller 6 and bulb 7, and dioxy is fixed in the internal side wall of the incubator 1
Change concentration of carbon sensor 10 and oxygen concentration sensor 11, the PLC 9 fixed in the exterior side wall of the incubator 1,
The PLC 9 electrically connects with the gas concentration lwevel sensor 10 and the oxygen concentration sensor 11, the culture
Dissolved oxygen sensor 1 and acdity and basicity measuring meter 19 are set in ware 1, and the inner bottom surface of incubator 1 fixes culture dish two
18, dissolved oxygen sensor 2 17 is set in the culture dish 2 18, the PLC 9 and the dissolved oxygen sensor 1,
The acdity and basicity measuring meter 19 and the dissolved oxygen sensor 2 17 are electrical connection, the PLC 9 and the oxygen gas
Amount controller 3, tolerance adjustment control device 4, the temperature controller 6, the bulb 7 and the electric rotating machine 15 are electricity
Connection.
Inflation inlet, the tolerance are respectively provided with the oxygen gas cylinder 2, the nitrogen gas cylinder 5 and the dioxide bottle 8
Gas outlet is set on adjustment control device 4.
The inner bottom surface of incubator 1 fixes the culture dish 2 18, and the PLC is 51 single-chip microcomputers.
Operation principle：A kind of cell culture apparatus for strengthening oxygen supply provided by the utility model, PLC can be total
Body controls the operation of whole incubator, is adjusted by tolerance adjustment control device into nitrogen, oxygen and titanium dioxide in incubator
The ratio of carbon, the dense of carbon dioxide and oxygen in incubator is monitored by gas concentration lwevel sensor and oxygen concentration sensor
Degree.
Oxygen tolerance in the nutrient solution for squeezing into culture dish one is adjusted by oxygen air flow controller, sensed by dissolved oxygen
Device one come monitor experiment nutrient solution in dissolved oxygen concentration, by dissolved oxygen sensor two come monitor contrast nutrient solution in dissolved oxygen
Concentration, the acid-base value in nutrient solution is monitored by acdity and basicity measuring meter；The speed of chassis rotation is controlled by electric rotating machine, makes oxygen
Gas can be squeezed into equably in experiment nutrient solution, and temperature controller can control the temperature in incubator, and bulb can illuminate, nitrogen
Gas bottle, oxygen gas cylinder and dioxide bottle can be inflated from inflation inlet.
In summary, the utility model can not only meet the environment needed for general cell culture, additionally it is possible to especially enhancing
The supply of oxygen cell, it is greatly convenient to be provided for researcher's research aerobic cell.
Disclosed above is only a specific embodiment of the present utility model, and still, the utility model embodiment is not office
It is limited to this, the changes that any person skilled in the art can think of should all fall into the scope of protection of the utility model.
Claims (3)
1. a kind of cell culture apparatus for strengthening oxygen supply, it is characterised in that including incubator (1) and PLC (9), institute
State incubator (1) inner bottom surface and fix pole (16), pole (16) the top mounting rotary electric machine (15), the electric rotating machine
(15) fixed underpan (14) in rotating shaft, culture dish one (13), the private side of the incubator (1) are fixed on the chassis (14)
Fixed oxygen gas bottle (2), nitrogen gas cylinder (5), dioxide bottle (8) and tolerance adjustment control device (4), the oxygen are separated on wall
Gas bottle (2), the nitrogen gas cylinder (5) and the dioxide bottle (8) pass through tracheae and the tolerance adjustment control device
(4) connect, the oxygen gas cylinder (2) connects oxygen air flow controller (3) by tracheae, and the oxygen air flow controller (3) is logical
Tracheae is crossed to be passed through in the nutrient solution of the culture dish one (13), incubator (1) inner tip installation temperature controller (6) and
Bulb (7), gas concentration lwevel sensor (10) and oxygen concentration sensor are fixed in the internal side wall of the incubator (1)
(11) PLC (9), the PLC (9) and the dioxy, are fixed in the exterior side wall of the incubator (1)
Change concentration of carbon sensor (10) and the oxygen concentration sensor (11) electrical connection, dissolved oxygen is set in the culture dish one (13)
Sensor one (12) and acdity and basicity measuring meter (19), incubator (1) inner bottom surface fix culture dish two (18), the culture
Dissolved oxygen sensor two (17), the PLC (9) and the dissolved oxygen sensor one (12), institute are set in ware two (18)
It is electrical connection, the PLC (9) and the oxygen to state acdity and basicity measuring meter (19) and the dissolved oxygen sensor two (17)
Gas air flow controller (3), the tolerance adjustment control device (4), the temperature controller (6), the bulb (7) and the rotation
Motor (15) is electrical connection.
2. device as claimed in claim 1, it is characterised in that the oxygen gas cylinder (2), the nitrogen gas cylinder (5) and described
Inflation inlet is respectively provided with dioxide bottle (8), gas outlet is set on the tolerance adjustment control device (4).
3. device as claimed in claim 1, it is characterised in that incubator (1) inner bottom surface fixes the culture dish two
(18), the PLC is 51 single-chip microcomputers.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720905423.7U CN207159236U (en) | 2017-07-25 | 2017-07-25 | A kind of cell culture apparatus for strengthening oxygen supply |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720905423.7U CN207159236U (en) | 2017-07-25 | 2017-07-25 | A kind of cell culture apparatus for strengthening oxygen supply |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207159236U true CN207159236U (en) | 2018-03-30 |
Family
ID=61713846
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720905423.7U Expired - Fee Related CN207159236U (en) | 2017-07-25 | 2017-07-25 | A kind of cell culture apparatus for strengthening oxygen supply |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207159236U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109810900A (en) * | 2019-03-12 | 2019-05-28 | 华道(上海)生物医药有限公司 | Totally-enclosed cell culture gas control system |
| CN112608847A (en) * | 2021-01-27 | 2021-04-06 | 张海甄 | Sealed cultivation device for biological cell cultivation and use method thereof |
-
2017
- 2017-07-25 CN CN201720905423.7U patent/CN207159236U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109810900A (en) * | 2019-03-12 | 2019-05-28 | 华道(上海)生物医药有限公司 | Totally-enclosed cell culture gas control system |
| CN109810900B (en) * | 2019-03-12 | 2024-01-12 | 华道(上海)生物医药有限公司 | Totally-enclosed cell culture gas control system |
| CN112608847A (en) * | 2021-01-27 | 2021-04-06 | 张海甄 | Sealed cultivation device for biological cell cultivation and use method thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180330 Termination date: 20210725 |
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| CF01 | Termination of patent right due to non-payment of annual fee |