CN206715966U

CN206715966U - A kind of bioanalysis micro-fluidic detection device

Info

Publication number: CN206715966U
Application number: CN201720466957.4U
Authority: CN
Inventors: 刘玲; 杨海霞
Original assignee: Individual
Current assignee: Individual
Priority date: 2017-04-28
Filing date: 2017-04-28
Publication date: 2017-12-08
Anticipated expiration: 2027-04-28

Abstract

The utility model is applied to medical detection technology, provide a kind of bioanalysis micro-fluidic detection device, including substrate, cover plate is bonded with the substrate, reagent one and reagent two have been arranged side by side on the cover plate, the reagent one is respectively communicated with a microchannel with the bottom of reagent two, the branch microchannel is arranged on substrate, the two branch microchannels are converged at blender, and the mixer outlet sets four points of microchannels, described to divide setting biological specimen unit on microchannel；The blender includes V-type microchannel, two microchannels are pooled a main microchannel by the V-type microchannel, several shunting posts are additionally provided among the main microchannel, the shunting linear array distribution of post, the shunting post is rhombus, the acute angle of the rhombus is 60 °, an acute angle directional liquid flow direction of the rhombus.Reagent can be sufficiently well mixed by the utility model, while the convenient multisample that carries out detects simultaneously, and detection speed improves.

Description

A kind of bioanalysis micro-fluidic detection device

Technical field

It the utility model is related to medical detection technology, more particularly to a kind of bioanalysis micro-fluidic detection device.

Background technology

Micro-fluidic chip is a kind of system for manipulating minute yardstick fluid and being flowed in minim channel or structure, and it can be one A biological or chemical laboratory is integrated on the individual small thin slice to only several square centimeters.Micro-fluidic chip can realize sample Consumption is greatly decreased, i.e. cost is greatly reduced, and reduces sample processing time, improves detection resolution/sensitivity, for The development of modern science and technology has far-reaching influence and meaning.Micro-fluidic chip is widely used in medical diagnosis on disease, drug screening, ring at present The fields such as border detection, food security.

Such as when carrying out bioanalysis using micro-fluidic chip, the identical same detection device of biological specimen, often occur Detection data difference is big, and error is big, and the result to bioanalysis causes greatly to perplex, and traces it to its cause as existing micro-fluidic chip Reagent mixing is uneven to influence testing result；In addition, in multisample contrasting detection, it need to carry out, see on multiple micro-fluidic chips It need to one by one be carried out when examining comparative analysis, contrast unobvious, due to needing largely to be acted, detection speed is slow, especially when more Easily cause to detect data obfuscation during sample.

In summary, prior art there will naturally be inconvenience and defect in actual use, and it is therefore necessary to improved.

Utility model content

For it is above-mentioned the defects of, the purpose of this utility model is to provide a kind of bioanalysis micro-fluidic detection device, Reagent can be sufficiently well mixed by it, be prevented because reagent mixed problem causes to detect data deviation, while conveniently enter Row multisample detects simultaneously, not only facilitates contrast, prevents from obscuring, and multisample detects simultaneously, and detection speed improves.

To achieve these goals, the utility model provides a kind of bioanalysis micro-fluidic detection device, including substrate, Be bonded with cover plate on the substrate, be arranged side by side reagent one and reagent two on the cover plate, the reagent one with The bottom of reagent two is respectively communicated with a microchannel, and the branch microchannel is arranged on substrate, and the two branch microchannels are mixed Converge at clutch, the mixer outlet sets four points of microchannels, described to divide setting biological specimen unit on microchannel；It is described Blender includes V-type microchannel, and two microchannels are pooled a main microchannel, the main microchannel by the V-type microchannel Centre is additionally provided with several shunting posts, and the shunting linear array distribution of post, the shunting post is rhombus, the rhombus it is sharp Angle is 60 °, an acute angle directional liquid flow direction of the rhombus.

As preferable technical scheme, the cross section of reagent one is circle, and the cross section of reagent two is rectangular Shape, the volume of the reagent one are equal with the volume of reagent two.

As preferable technical scheme, the cover plate is two-stage step shape structure, and the reagent one is set with reagent two Put on the cover plate higher step face.

As preferable technical scheme, Micropump and micro-valve door are set on the branch microchannel.

As preferable technical scheme, micro-valve door is additionally provided with each point microchannel, controls the switch of the micro-valve door It can control the break-make of each point of microchannel.

As preferable technical scheme, the biological specimen unit is independent bonding chip, and life is provided with the chip Thing molecule film.

Reagent can be sufficiently well mixed by the utility model, be prevented because reagent mixed problem causes to detect data Deviation, while the convenient multisample that carries out detects simultaneously, not only facilitates contrast, prevents from obscuring, and also multisample detects simultaneously, detection Speed improves.

Brief description of the drawings

Fig. 1 is structural representation of the present utility model；

Fig. 2 is Fig. 1 A-A sectional views；

Fig. 3 is blender enlarged drawing in Fig. 1.

In figure, 1- substrates, 2- cover plates, 3- reagents one, 4- reagents two, 5- branch microchannel, 6- Micropumps, 7- micro-valves Door, 8- blenders, 9- divide microchannel, 10- biological specimen units, 81-V types microchannel, the main microchannels of 82-, 83- shunting posts.

Embodiment

In order that the purpose of this utility model, technical scheme and advantage are more clearly understood, below in conjunction with accompanying drawing and implementation Example, the utility model is further elaborated.It should be appreciated that specific embodiment described herein is only explaining The utility model, it is not used to limit the utility model.

Referring to Fig. 1, Fig. 2, Fig. 3, the utility model provides a kind of bioanalysis micro-fluidic detection device.The device bag Substrate 1 is included, cover plate 2 is bonded with the substrate 1, the cover plate 2 is two-stage step shape structure, in the higher step of cover plate 2 Reagent 1 and reagent 24 are arranged side by side on face, the cross section of reagent 1 is circle, and the reagent 24 is horizontal Section is rectangle, and the volume of the reagent 1 is equal with the volume of reagent 24.

The bottom of the reagent 1 and reagent 24 is respectively communicated with branch microchannel 5, and the branch microchannel 5 is arranged on base On piece 1, Micropump 6 and micro-valve door 7, the two branch microchannels 5 are set to converge at blender 8 on the branch microchannel 5, it is described mixed The outlet of clutch 8 sets four points of microchannels 9, described to divide setting biological specimen unit 10 on microchannel 9.

The blender 8 includes V-type microchannel 81, and it is micro- that two microchannels 5 are pooled a master by the V-type microchannel 81 Passage 82, several shunting posts 83 are additionally provided among the main microchannel 82, and the shunting 83 linear array distribution of post is described Shunting post 83 is rhombus, and the sharp angle α of the rhombus is 60 °, an acute angle directional liquid flow direction of the rhombus.

Micro-valve door 7 is additionally provided with each point microchannel 9, controls each point of microchannel 9 of switch controls of the micro-valve door 7 Break-make.

The biological specimen unit 10 is independent bonding chip, and biomolecule film is provided with the chip.

In summary, reagent can be sufficiently well mixed by the utility model, be prevented because reagent mixed problem is led Detection data deviation is caused, while the convenient multisample that carries out detects simultaneously, not only facilitates contrast, prevents from obscuring, and multisample is same When detect, detection speed improve.

Certainly, the utility model can also have other various embodiments, without departing substantially from the utility model spirit and its essence In the case of, those skilled in the art work as can make various corresponding changes and deformation according to the utility model, but these Corresponding change and deformation should all belong to the scope of the claims appended by the utility model.

Claims

1. a kind of bioanalysis micro-fluidic detection device, it is characterised in that including substrate（1）, the substrate（1）On be bonded with Cover plate（2）, in the cover plate（2）On reagent one has been arranged side by side（3）And reagent two（4）, the reagent one（3）With examination Agent pond two（4）Bottom be respectively communicated with a microchannel（5）, the branch microchannel（5）It is arranged on substrate（1）On, two branch Microchannel（5）In blender（8）Place converges, the blender（8）Outlet sets four points of microchannels（9）, it is described to divide microchannel （9）Upper setting biological specimen unit（10）；The blender（8）Including V-type microchannel（81）, the V-type microchannel（81）Will Two microchannels（5）Pool a main microchannel（82）, the main microchannel（82）Centre is additionally provided with several shunting posts （83）, the shunting post（83）Linear array distribution, the shunting post（83）For rhombus, the acute angle of the rhombus is 60 °, institute State an acute angle directional liquid flow direction of rhombus.

2. bioanalysis according to claim 1 micro-fluidic detection device, it is characterised in that the reagent one（3） Cross section is circular, the reagent two（4）Cross section is rectangle, the reagent one（3）Volume and reagent two（4） Volume it is equal.

3. bioanalysis according to claim 1 micro-fluidic detection device, it is characterised in that the cover plate（2）For two Level step-like structure, the reagent one（3）With reagent two（4）It is arranged on the cover plate（2）On higher step face.

4. bioanalysis according to claim 1 micro-fluidic detection device, it is characterised in that the branch microchannel（5） Upper setting Micropump（6）With micro-valve door（7）.

5. bioanalysis according to claim 1 micro-fluidic detection device, it is characterised in that divide microchannel described in each （9）On be additionally provided with micro-valve door（7）, control the micro-valve door（7）Each point of microchannel of switch controls（9）Break-make.

6. bioanalysis according to claim 1 micro-fluidic detection device, it is characterised in that the biological specimen unit （10）For independent bonding chip, the chip is interior to be provided with biomolecule film.