CN206696185U - Organophosphorus pesticide residue rapid detection device - Google Patents
Organophosphorus pesticide residue rapid detection device Download PDFInfo
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- CN206696185U CN206696185U CN201720383805.8U CN201720383805U CN206696185U CN 206696185 U CN206696185 U CN 206696185U CN 201720383805 U CN201720383805 U CN 201720383805U CN 206696185 U CN206696185 U CN 206696185U
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- 238000001514 detection method Methods 0.000 title claims abstract description 24
- 239000003987 organophosphate pesticide Substances 0.000 title claims abstract description 18
- 230000003287 optical effect Effects 0.000 claims abstract description 12
- 239000013307 optical fiber Substances 0.000 claims abstract description 12
- 238000012545 processing Methods 0.000 claims abstract description 5
- 239000008236 heating water Substances 0.000 claims description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 16
- 108091000080 Phosphotransferase Proteins 0.000 claims description 15
- 102000020233 phosphotransferase Human genes 0.000 claims description 15
- 230000001105 regulatory effect Effects 0.000 claims description 15
- 230000002401 inhibitory effect Effects 0.000 claims description 5
- 230000003993 interaction Effects 0.000 claims description 5
- 229920001875 Ebonite Polymers 0.000 claims description 2
- RRHGJUQNOFWUDK-UHFFFAOYSA-N Isoprene Chemical compound CC(=C)C=C RRHGJUQNOFWUDK-UHFFFAOYSA-N 0.000 claims description 2
- 238000003780 insertion Methods 0.000 claims description 2
- 230000037431 insertion Effects 0.000 claims description 2
- 230000005622 photoelectricity Effects 0.000 claims description 2
- 229920001195 polyisoprene Polymers 0.000 claims description 2
- 230000001276 controlling effect Effects 0.000 claims 1
- 238000013500 data storage Methods 0.000 claims 1
- 230000005611 electricity Effects 0.000 claims 1
- 238000006243 chemical reaction Methods 0.000 abstract description 4
- 238000001914 filtration Methods 0.000 abstract description 2
- 230000003750 conditioning effect Effects 0.000 abstract 2
- 230000003321 amplification Effects 0.000 abstract 1
- 230000005540 biological transmission Effects 0.000 abstract 1
- 230000005764 inhibitory process Effects 0.000 abstract 1
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 1
- 239000000447 pesticide residue Substances 0.000 abstract 1
- 238000000034 method Methods 0.000 description 10
- 102000004190 Enzymes Human genes 0.000 description 6
- 108090000790 Enzymes Proteins 0.000 description 6
- 238000010586 diagram Methods 0.000 description 4
- 238000012360 testing method Methods 0.000 description 3
- 230000000052 comparative effect Effects 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 229920001971 elastomer Polymers 0.000 description 2
- 230000005693 optoelectronics Effects 0.000 description 2
- 241000208340 Araliaceae Species 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000004026 adhesive bonding Methods 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000011017 operating method Methods 0.000 description 1
- 238000004088 simulation Methods 0.000 description 1
- 230000003595 spectral effect Effects 0.000 description 1
- 238000003805 vibration mixing Methods 0.000 description 1
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- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The utility model relates to the technical field of pesticide residue detection, in particular to a device for rapidly detecting organophosphorus pesticide residue, which comprises a power supply transmission module, a laser, an optical fiber branching unit, a collimator, a photoelectric receiving tube, a signal conditioning module and a main control module; the power supply transmitting module supplies power for other modules, laser generated by the laser is changed into a multi-path light source through an optical fiber branching unit and then is connected into the collimator, light collimated by the collimator is received by the photoelectric receiving tube, a cuvette is arranged on an optical channel between the collimator and the photoelectric receiving tube, a signal conditioning module carries out photoelectric conversion and filtering amplification processing on a light source signal received by the photoelectric receiving tube and then outputs the light source signal to the main control module, and the main control module obtains the sample inhibition rate after calculation. The laser is adopted as a light source, so that the stability and reliability are improved, the laser is divided into a plurality of beams by the optical fiber branching unit, the consistency of the light is ensured, and the data obtained by subsequent processing is more accurate.
Description
Technical field
The residual detection technique field of agriculture is the utility model is related to, more particularly to a kind of organophosphorus pesticide quick detection dress
Put.
Background technology
At present, it is former with spectral absorption to be all based on enzyme level in the market main flow organophosphorus pesticide device for fast detecting
Reason, realizes the sxemiquantitative of detection process.In detection process, enzyme it is active most important, and temperature be influence enzyme decision because
Element, therefore typically require that temperature must be constant during actually detected.There is many deficiencies in existing detection means, wherein most
It is important that in terms of optical texture, detection light source use LED point light source more, and light source has fluctuation and variant, and then introducing
Uncertain factor, interference detection results.
Utility model content
The purpose of this utility model is to provide organophosphorus pesticide residual fast speed detector, ensures the uniformity of light source,
Ensure the accuracy of testing result.
To realize object above, the technical solution adopted in the utility model is：A kind of organophosphorus pesticide quick detection
Device, including power supply transmitting module, laser, optical fiber splitter, collimater, photoelectric receiving tube, Signal-regulated kinase and master
Control module；Described power supply transmitting module is laser, Signal-regulated kinase and main control module power supply, is swashed caused by laser
Light is accessed in collimater after optical fiber splitter becomes multichannel light source, and the light after collimator collimation is by photoelectric receiving tube institute
Receive, be disposed with cuvette on the optical channel between collimater and photoelectric receiving tube, Signal-regulated kinase is by photoelectric receiving tube
To main control module, main control module obtains after being computed for output after the light signal received carries out opto-electronic conversion, filter amplifying processing
To sample inhibiting rate.
Compared with prior art, there is following technique effect in the utility model：Here compared using laser as light source
It is more reliable and stable for LED electric light sources, meanwhile, laser is divided into by multi beam by optical fiber splitter, ensured by each comparing
The light of color ware is consistent, so ensures accuracy, the accuracy of data obtained by subsequent treatment.
Brief description of the drawings
Fig. 1 is theory diagram of the present utility model；
Fig. 2 is optical detecting platform structural representation, collimater and photelectric receiver not shown in it；
Fig. 3 is heating water bath cellular construction schematic diagram；
Fig. 4 is main control module theory diagram of the present utility model；
Fig. 5 is Signal-regulated kinase circuit diagram of the present utility model；
Fig. 6 is the utility model control flow chart.
Embodiment
With reference to Fig. 1 to Fig. 6, the utility model is described in further detail.
Refering to Fig. 1, a kind of organophosphorus pesticide device for fast detecting, including power supply transmitting module 10, laser 20,
Optical fiber splitter 30, collimater 41, photoelectric receiving tube 42, Signal-regulated kinase 50 and main control module 60；Described power supply becomes
Module 10 is sent to be powered for laser 20, Signal-regulated kinase 50 and main control module 60, laser caused by laser 20 passes through light
Fine shunt 30 is accessed in collimater 41 after becoming multichannel light source, and the light after the collimation of collimator 41 is by the institute of photoelectric receiving tube 42
Receive, be disposed with cuvette on the optical channel between collimater 41 and photoelectric receiving tube 42, Signal-regulated kinase 50 is by photoelectricity
The light signal that reception pipe 42 receives is exported to main control module 60, main control module after carrying out opto-electronic conversion, filter amplifying processing
60 be computed after obtain sample inhibiting rate.By setting laser 20 and optical fiber splitter 30 so that each road light source is all very unified,
The interference of multiple light courcess is avoided, laser is divided into 5 tunnels by optical fiber splitter 30 in the present embodiment, and being specifically divided into several roads can be as needed
Selected.
In the prior art, in detection operates, it is necessary to manually enter after sound measuring samples solution addition specific reaction reagent
Row vibration mixing, artificial vibration are mixed some following deficiency：First, vibration is not uniform enough, process can not control well；
Second, labor intensive resource.As preferred scheme of the present utility model, described collimater 41, photoelectric receiving tube 42 are fixed
On optical detecting platform 40, below optical detecting platform 40 or side is provided with the vibration unit for driving cuvette to act
43, power supply transmitting module 10 be vibration unit 43 power, the output control signal of main control module 60 control vibration unit 43 startup/
Stop.Unit 43 is shaken by setting so that can automatically be vibrated in detection process, without manual operation, improve device
Automaticity.
During detection, enzyme it is active most important, and temperature is to influence the determinant of enzyme, therefore in reality
Require that temperature must be constant in detection process, existing device is all equipped with single water bath heating device, is all deposited using with carrying
In very big inconvenience.In the present embodiment, it is preferable that also including heating water bath unit 70, power supply transmitting module 10 is heating water bath
Unit 70 is powered, and main control module 60 gathers the temperature of the reclaimed water of heating water bath unit 70 and output control signal control heating water bath list
Startup/stopping of member 70.Similarly, heating water bath unit 70 is controlled by main control module 60 as the case may be, can improves and be
The automaticity of system, convenient use.
It should be noted that above-mentioned vibration unit 43 and heating water bath unit 70 also can be individually controlled, so
Corresponding function can equally be realized.
Refering to Fig. 2, it is preferable that be fixedly installed square block 44 on described optical detecting platform 40, square block 44 is along width
Degree direction offers square hole 441, and the both ends of square hole 441 insertion square is set, and collimater 41, photoelectric receiving tube 42 are placed in square hole
441 both ends, the light come out from collimater 41 are received after square hole 441 by photoelectric receiving tube 42；Except one of side
The groove 442 for accommodating cuvette is offered in other square holes 441 outside hole 441.The setting of square block 44, is mainly used for
Fixed cuvette, meanwhile, in order to avoid the light that collimater 41 projects can be entered in photoelectric receiving tube 42, open up here
Square hole 441.Here it is the fluctuation situation for detecting light source to have a square hole for not opening up groove 442 441, and other roads pass through band
The passage for having cuvette is received by photoelectric receiving tube 42, for calculating the inhibiting rate of sample.
The structure of vibration unit 43 has a variety of designs, provides a kind of more preferred embodiment here for ginseng
Examine：Described vibration unit 43 includes flat board 431, direct current generator 432, and the surrounding of flat board 431 is screwed in optics inspection
Survey on platform 40, the central region of flat board 431 raises up, and the bottom of square block 44 is screwed in the projection of flat board 431
Region on；Direct current generator 432 is arranged between square block 44 and flat board 431, the upper end of direct current generator 432 by vulcanie with
Square block 44 is connected, and its lower end is fixed on the raised region of flat board 431 by elastic caoutchouc, is set on the axle of direct current generator 432
It is equipped with eccentric block.The power resources of vibration are the direct current generators 432 with eccentric block, among flat board 431 it is convex be in order to
Improve the effect of vibration.When mounted, first square block 44 can be screwed on flat board 431, then by hard rubber
The direct current generator 432 for sticking good rubber on direct current generator 432, is finally embedded in square block by glue and soft rubber by two-sided gluing
Between 44 and flat board 431, both ends respectively place one, are allowed to clamping.
Refering to Fig. 3, similarly, the structure of heating water bath unit 70 also has many kinds, provides here a kind of more preferable
Embodiment, described heating water bath unit 70 include water bath 71, support 72, temperature sensor 73 are placed with water bath 71
And electric heater 74；Support 72 is in the square box shape of upper opening, and the cassette bottom of square box offers groove and be used for for en plaque and cassette bottom
Place cuvette, the side wall of square box is netted, thus facilitates and the cuvette in square box is heated, at the box mouth of square box to
Outside is provided with the notch that flange is used to ride upon water bath 71, is so easy to taking for support 72 after design；TEMP
Device 73 and electric heater 74 are arranged on the lower section of support 72 and temperature sensor 73 and electric heater 74 are electric with main control module 60
Connection.
Refering to Fig. 4, as preferred scheme of the present utility model, described main control module 60 include chip S3C6410 and
Power circuit, reset circuit, oscillating circuit, A/D change-over circuits, PWM output circuits, the simulation that Signal-regulated kinase 50 exports
Signal is exported after A/D change-over circuits are converted into data signal to chip S3C6410, and PWM output circuits, which are used to control, to be vibrated
Unit 43 is vibrated and the water temperature of heating water bath unit 70.Further, described main control module 60 also includes memory
Circuit, touch screen circuitry, serial port circuit and mixed-media network modules mixed-media, memory circuitry are used to store data, touch screen circuitry
For man-machine interaction, serial port circuit is used to realize that the data between mixed-media network modules mixed-media and chip S3C6410 to communicate, and mixed-media network modules mixed-media is used for
Realize high in the clouds storage, the remote control of PC ends or mobile terminal of data.Human-computer interaction interface typically selects touch-screen, in order to just
Control instruction is inputted, parameter is adjusted and facilitates main control module 60 by other units or the state of module in operating personnel
Information is sent on human-computer interaction interface and shown, there is provided touch screen circuitry here.
Refering to Fig. 5, specifically, described Signal-regulated kinase 50 include I/V change-over circuits 51, low-pass filter circuit 52 with
And see-saw circuit 53；I/V change-over circuits 51 include chip MAX4488, and chip MAX4488 in-phase input end is grounded, its
Inverting input is by passing sequentially through resistance R1, photodiode D1 is followed by power supply VCC, and two after resistance R2 and electric capacity C1 parallel connections
The inverting input with chip MAX4488, output end are connected respectively at end；Low-pass filter circuit 52 includes chip OP07, chip
OP07 in-phase input end is grounded by electric capacity C2, and its in-phase input end also passes through resistance R4, R3 and chip MAX4488 output
End is connected, and is connected after drawing a branch road between resistance R3, R4 by electric capacity C3 with chip OP07 output end, chip OP07's
Inverting input is grounded by resistance R5 and is connected by resistance R6 with chip OP07 output end；See-saw circuit 53 includes
Chip AT24LC04, chip AT24LC04 in-phase input end are grounded by resistance R8, its inverting input by resistance R7 with
Chip OP07 output end is connected and is connected by resistance R9 with chip AT24LC04 output end, chip AT24LC04 output
The negative pole also with diode D2 is held to be connected, diode D2 plus earth, chip AT24LC04 output end is as signal condition
The output end of module 50 is connected with the input of A/D change-over circuits.
Here is specifically to detect operating procedure using the present apparatus：
(1) 1g detected samples are taken, are put into container, add 5mL extract solutions, stand 10min, notably sample size
To ensure that sample can be immersed in extract solution.Opening detecting device simultaneously, the water temperature of heating water bath unit 70 is allowed to reach default temperature
Degree.
(2) cuvette (quantity determines according to the quantity of measuring samples) is taken, is respectively dropped into 0.1mL enzyme.
(3) resulting solution in step (1) is filtered, takes the solution 2.5mL after filtering, as sample extracting solution, separately
2.5mL extract solutions, as comparative solution are taken, they are separately added into step (2) described cuvette.
(4) when the water temperature stability of heating water bath unit 70 is in default water temperature, the ratio of solution will be carried step (3) Suo Shu
Color ware is put into heating water bath unit 70, is taken out after placing 10min.
(5) gained cuvette wiped clean, the cuvette equipped with sample extracting solution in step (4) are put into sense channel
In, the cuvette equipped with comparative solution is put into contrast passage, adds 0.1mL developers and 0.1mL bottoms thereto successively respectively
Thing.
(6) click on human-computer interaction interface and start detection button, vibration unit 43 starts, and starts to detect after vibrating 10s.
(7) data obtained when detecting are directly over Signal-regulated kinase 50 and exported to main control module 60, main control module 60
The inhibiting rate of measuring samples is calculated.
Refering to Fig. 6, from said process it will be seen that the flow of present apparatus operation mainly has two parts, a part
For heating water bath control flow, current water temperature is detected first, compared with preset temperature, if the two is equal, it indicates that lamp is bright,
Indicator lamp goes out if not waiting, and calls PID algorithms to adjust current water temperature.Another part is sample detection flow, is selected successively first
Passage and sample type are selected.To click on and start detection button, first vibrate 10s, then start to detect, detection process continues 220s,
Period can stop at any time.After detection terminates, testing result will be shown, can choose whether to delete afterwards, check, send number
According to historical data can also be checked.Program has reserved network interface simultaneously, and above-mentioned all operations can also pass through high in the clouds PC and movement
Terminal App is realized.
In a word, the present apparatus uses single laser light source, and five road light sources are divided into after optical fiber splitter, effectively overcome light more
The uncertain interference that source is brought, makes testing result more accurate.In addition, the present apparatus is also integrated with heating water bath unit 70, shaken
Moving cell 43, make whole detection process convenient, fast.In addition, by Configuration network module, the high in the clouds that detection data can be achieved passes
The defeated and remote control of detection process.Therefore, the present apparatus has good application in organophosphorus pesticide field of fast detection
Prospect.
Claims (9)
- A kind of 1. organophosphorus pesticide device for fast detecting, it is characterised in that：Including power supply transmitting module (10), laser (20), optical fiber splitter (30), collimater (41), photoelectric receiving tube (42), Signal-regulated kinase (50) and main control module (60)；Described power supply transmitting module (10) is that laser (20), Signal-regulated kinase (50) and main control module (60) are powered, Laser caused by laser (20) is accessed in collimater (41) after optical fiber splitter (30) becomes multichannel light source, collimator (41) light after collimating is received by photoelectric receiving tube (42), and the optics between collimater (41) and photoelectric receiving tube (42) leads to Cuvette is disposed with road, the light signal that Signal-regulated kinase (50) receives photoelectric receiving tube (42) carries out photoelectricity and turned Change, output to main control module (60), main control module (60) obtains sample inhibiting rate after being computed after filter amplifying processing.
- 2. organophosphorus pesticide device for fast detecting as claimed in claim 1, it is characterised in that：Described collimater (41), photoelectric receiving tube (42) is each attached on optical detecting platform (40), and below optical detecting platform (40) or side is set There is the vibration unit (43) for driving cuvette to act, power supply transmitting module (10) is powered for vibration unit (43), master control mould Startup/stopping of block (60) output control signal control vibration unit (43).
- 3. organophosphorus pesticide device for fast detecting as claimed in claim 1, it is characterised in that：Also include heating water bath list First (70), power supply transmitting module (10) are powered for heating water bath unit (70), main control module (60) collection heating water bath unit (70) startup/stopping of the temperature of reclaimed water and output control signal control heating water bath unit (70).
- 4. organophosphorus pesticide device for fast detecting as claimed in claim 2, it is characterised in that：Described optical detection is put down Square block (44) is fixedly installed on platform (40), square block (44) offers square hole (441), square hole (441) two in the width direction End insertion square is set, and collimater (41), photoelectric receiving tube (42) are placed in the both ends of square hole (441), go out from collimater (41) The light come is received after square hole (441) by photoelectric receiving tube (42)；Other square holes in addition to one of square hole (441) (441) groove (442) for accommodating cuvette is offered in.
- 5. organophosphorus pesticide device for fast detecting as claimed in claim 4, it is characterised in that：Described vibration unit (43) flat board (431), direct current generator (432) are included, the surrounding of flat board (431) is screwed in optical detecting platform (40) On, the central region of flat board (431) raises up, and the bottom of square block (44) is screwed in flat board (431) projection On region；Direct current generator (432) is arranged between square block (44) and flat board (431), and direct current generator (432) upper end passes through hard Rubber is connected with square block (44), and its lower end is fixed on the raised region of flat board (431) by elastic caoutchouc, direct current generator (432) eccentric block is provided with axle.
- 6. organophosphorus pesticide device for fast detecting as claimed in claim 3, it is characterised in that：Described heating water bath list First (70) include water bath (71), and support (72), temperature sensor (73) and electric heater are placed with water bath (71) (74)；Support (72) is in the square box shape of upper opening, and the cassette bottom of square box offers groove and be used to place colorimetric for en plaque and cassette bottom Ware, the side wall of square box is netted, is provided with the notch that flange is used to ride upon water bath (71) at the box mouth of square box laterally；Temperature Degree sensor (73) and electric heater (74) are arranged on lower section and temperature sensor (73) and the electric heater (74) of support (72) Electrically connected with main control module (60).
- 7. the organophosphorus pesticide device for fast detecting as described in claim any one of 1-6, it is characterised in that：Described master Controlling module (60) includes chip S3C6410 and power circuit, reset circuit, oscillating circuit, A/D change-over circuits, PWM output electricity Road, the analog signal of Signal-regulated kinase (50) output are exported to chip after A/D change-over circuits are converted into data signal S3C6410, PWM output circuit are used to control vibration unit (43) to be vibrated and the water temperature of heating water bath unit (70).
- 8. organophosphorus pesticide device for fast detecting as claimed in claim 7, it is characterised in that：Described main control module (60) memory circuitry, touch screen circuitry, serial port circuit and mixed-media network modules mixed-media are also included, memory circuitry is used to carry out data Storage, touch screen circuitry are used for man-machine interaction, and serial port circuit is used to realize that the data between mixed-media network modules mixed-media and chip S3C6410 are led to Letter, mixed-media network modules mixed-media are used for the remote control for realizing the high in the clouds storage of data, PC ends or mobile terminal.
- 9. organophosphorus pesticide device for fast detecting as claimed in claim 7, it is characterised in that：Described signal condition mould Block (50) includes I/V change-over circuits (51), low-pass filter circuit (52) and see-saw circuit (53)；I/V change-over circuits (51) include chip MAX4488, chip MAX4488 in-phase input end ground connection, and its inverting input leads to Cross and pass sequentially through resistance R1, photodiode D1 is followed by power supply VCC, the both ends after resistance R2 and electric capacity C1 parallel connections respectively with chip MAX4488 inverting input, output end are connected；Low-pass filter circuit (52) includes chip OP07, and chip OP07 in-phase input end is grounded by electric capacity C2, and it is the same as mutually defeated Enter end be also connected by resistance R4, R3 with chip MAX4488 output end, between resistance R3, R4 draw a branch road after by Electric capacity C3 is connected with chip OP07 output end, chip OP07 inverting input be grounded by resistance R5 and by resistance R6 with Chip OP07 output end is connected；See-saw circuit (53) includes chip AT24LC04, and chip AT24LC04 in-phase input end is grounded by resistance R8, Its inverting input is connected with chip OP07 output end by resistance R7 and by resistance R9 and chip AT24LC04 output End is connected, and the negative pole of chip AT24LC04 output end also with diode D2 is connected, diode D2 plus earth, chip AT24LC04 output end is connected as the output end of Signal-regulated kinase (50) with the input of A/D change-over circuits.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720383805.8U CN206696185U (en) | 2017-04-12 | 2017-04-12 | Organophosphorus pesticide residue rapid detection device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720383805.8U CN206696185U (en) | 2017-04-12 | 2017-04-12 | Organophosphorus pesticide residue rapid detection device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111189805A (en) * | 2019-11-27 | 2020-05-22 | 常州大学 | High-precision residual pesticide verification system |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111189805A (en) * | 2019-11-27 | 2020-05-22 | 常州大学 | High-precision residual pesticide verification system |
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