CN206573592U - A kind of test strips for detecting canine parainfluenza virus antibody - Google Patents
A kind of test strips for detecting canine parainfluenza virus antibody Download PDFInfo
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- CN206573592U CN206573592U CN201720266537.1U CN201720266537U CN206573592U CN 206573592 U CN206573592 U CN 206573592U CN 201720266537 U CN201720266537 U CN 201720266537U CN 206573592 U CN206573592 U CN 206573592U
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- parainfluenza virus
- canine parainfluenza
- test strips
- trace
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Abstract
The utility model discloses a kind of test strips for detecting canine parainfluenza virus antibody, including：Supporting layer, it includes multiple support blocks, and the side of each support block has inserting groove, and relative opposite side has plug division, the plug division of one of support block is inserted into the inserting groove of another support block in two neighboring support block, so that multiple support blocks are sequentially connected as an entirety；Detection layers, it includes being set in turn in sample adsorption fibrous layer, gold mark fibrous layer, cellulose film layer and absorbent material layer on supporting layer, wherein, detection trace and control trace are formed with cellulose film layer, the canine parainfluenza virus of colloid gold label or the restructuring canine parainfluenza virus HN albumen of colloid gold label are adsorbed with gold mark fibrous layer, detection trace is to be formed by the canine parainfluenza virus antigen liquid printing purified.Test strips of the present utility model can adjust length as needed, and high specificity, sensitivity is high, and easy, intuitively, accurately, applied widely, cost is low.
Description
Technical field
The utility model is related to the instrument of detection canine parainfluenza virus antibody, more particularly to a kind of detection canine parainfluenza virus
The test strips of antibody.
Background technology
It by canine parainfluenza virus (canine parainfluenza virus, CPIV) is to draw that canine parainfluenza virus infection, which is,
Play the important respiratory infectious disease of dog, to generate heat suddenly, coryza and bronchitis be characterized, all foster dogs in the whole world at present
Country nearly all have this sick prevalence.CPIV can infect the dog at various ages, and the pup state of an illness is heavier, and the death rate is higher.
Quick detection canine parainfluenza virus antibody, can not only evaluate immune effect of vaccine, and can be examined with adjuvant clinical
It is disconnected, it is the premise of effective control canine parainfluenza virus infection.The method of detection canine parainfluenza virus antibody mainly has serum at present
Neutralize the methods such as experiment, IFA and ELISA.However, with experiment, IFA and ELISA detection techniques complex operation, it is necessary to high in serum
Expensive instrument and equipment, process are longer, time and effort consuming, it is necessary to particular instrument equipment and professional and technical personnel's operation, it is difficult in basic unit
Popularization, it is impossible to meet the quick detection needs at production line or epidemic disease scene.It would therefore be highly desirable to there is a kind of quick detection dog parainfluenza
The equipment and technology of antiviral antibody.
Utility model content
For above-mentioned technical problem, the utility model can realize quick detection, convenient progress length there is provided a kind of
The test strips of the detection canine parainfluenza virus antibody of adjustment.
The utility model provide technical scheme be：
A kind of test strips for detecting canine parainfluenza virus antibody, including：
Supporting layer, it includes multiple support blocks, and the side of each support block has inserting groove, and relative opposite side, which has, to be inserted
The plug division of one of support block is inserted into the inserting groove of another support block in socket part, two neighboring support block, so that
Multiple support blocks are made to be sequentially connected as an entirety；
Detection layers, it includes the sample adsorption fibrous layer, gold mark fibrous layer, cellulose being set in turn on the supporting layer
Film layer and absorbent material layer, wherein, detection trace and control trace, the gold mark fiber are formed with the cellulose film layer
The canine parainfluenza virus of colloid gold label or the restructuring canine parainfluenza virus HN albumen of colloid gold label, the inspection are adsorbed with layer
It is to be formed by the canine parainfluenza virus antigen liquid printing purified to survey trace, and the control trace is by sheep or rabbit-anti dog parainfluenza virus
Malicious IgG solution printing is formed.
Preferably, in the test strips of described detection canine parainfluenza virus antibody, the multiple support block includes four
Support block, and the sample adsorption fibrous layer, gold mark fibrous layer, cellulose film layer and absorbent material layer be corresponding in turn to and be arranged on
In each support block.
Preferably, in the test strips of described detection canine parainfluenza virus antibody, the supporting layer is by hard plastic
Bar is made.
Preferably, in the test strips of described detection canine parainfluenza virus antibody, the sample adsorption fibrous layer is served as reasons
Mineral wool, nylon fiber or polyester fiber are made.
Preferably, in the test strips of described detection canine parainfluenza virus antibody, the gold mark fibrous layer is by glass
Cotton into.
Preferably, in the test strips of described detection canine parainfluenza virus antibody, the cellulose film layer is by nitric acid
Cellulose membrane, cellulose membrane, carboxymethyl cellulose film or polyvinylidene fluoride PVDF cellulose membranes are made.
Preferably, in the test strips of described detection canine parainfluenza virus antibody, the absorbent material layer blotting paper
It is made.
Preferably, it is the sample adsorption fibrous layer, described in the test strips of described detection canine parainfluenza virus antibody
Covered with the first protective layer on gold mark fibrous layer, covered with the second protective layer on the absorbent material layer, in the described first protection
Sample mark line is printed with layer.
Preferably, in the test strips of described detection canine parainfluenza virus antibody, when the shape in the cellulose film layer
When Cheng Youyi bars detect trace and a control trace, the arrangement mode of the detection trace and the control trace is | |, ten
Tenth, ┬ ┬, ┴ ┴, ├ ├, ┤ ┤ and ● ● any of；Traces are detected when being formed with two in the cellulose film layer
During with a control trace, two detection traces and a control trace are arranged in order, and arrangement mode is | | |, 10, ┬
┬ ┬, ┴ ┴ ┴, ├ ├ ├, ┤ ┤ ┤ and ● ● ● any of.
The test strips of detection canine parainfluenza virus antibody described in the utility model have the advantages that：
(1) structure that supporting layer is designed to be formed by connecting by multiple support blocks by the utility model, each support block
There is plug division and inserting groove in side relative to each other, two neighboring support block grafting each other, when needing to change test strips
Length, that is, change the length of supporting layer, it is only necessary to increases the number of support block as needed.The utility model is greatly improved
Test strips use upper comfort level.
(1) high specificity is detected, sensitiveness is high.The test strips that the utility model is provided are with colloid gold label dog parainfluenza virus
It is prepared from based on poison or colloid gold label restructuring canine parainfluenza virus HN albumen, gold grain and virus in gold mark virus protein
Formed between protein molecular without covalent bond, the two is combined by the Van der Waals force between the charges of different polarity, collaurum mark is to viral egg
White specificity and affinity influence very little, and with higher trace rate.
(2) it is easy to operate, quickly.Test strips of the present utility model are when in use without adding any other instrument and examination
Agent, as long as its test lead is inserted in measuring samples liquid 30 seconds or so, can determine that testing result in 5 minutes or so.
(3) result intuitive display, accurate.Test strips of the present utility model are printed with the detection trace and control for showing brownish red
Mark shows two brownish red traces that is, on cellulose membrane, represented in detected sample as the positive and negative trace of detection
In have antiviral antibody detection, be as a result the positive；Only one brownish red of display compares trace C on cellulose membrane, represents tested
Survey in sample liquid and do not detect antiviral antibody, be as a result feminine gender.Result judgement is directly perceived, accurate, simple and clear, is less prone to false negative
With false positive erroneous judgement.
(4) investment and testing cost are reduced.Test strips of the present utility model when in use, are not required to separately match somebody with somebody Other Instruments, set
Standby and reagent, saves big measuring appratus, equipment and additive reagent expense；Specialty and layman can carry out scene whenever and wherever possible
Detection, removes the travelling expenses of diagnosis room without paying expert diagnosis Laboratory Fee or feeding sample, saves testing cost, testing cost is low.
(5) have a wide range of application, be easy to popularity application.The test strips that the utility model is provided are simple to operate, into " a step
Formula " or " foolproof ", and be convenient for carrying and preserve, the need for different levels personnel can be met, including specialty chemical examination, customs's inspection
Epidemic disease, health and epidemic prevention, quality-monitoring, livestock products processing, intensive culture to individual cultivate etc., with wide market prospects and compared with
Good economical, societal benefits.
(6) the utility model detects antiviral antibody with immune chromatography test paper, utilizes micropore according to ELISA general principle
The diafiltration concentration of filter membrane and capillarity, antigen-antibody reaction is gone on solid phase filter membrane by ELISA Traditional liquid phase environment
It is quick to carry out, and enzyme trace is replaced using collaurum trace, with the colour developing situation of direct visual perception collaurum, examined immediately
Result is surveyed, therefore this method is more easier than serological methods such as ELISA, quick.
Brief description of the drawings
Fig. 1 is the top view of the test strips of detection canine parainfluenza virus antibody described in the utility model；
Fig. 2 is the lateral plan of the test strips of detection canine parainfluenza virus antibody described in the utility model；
Fig. 3 is the structural representation of supporting layer described in the utility model.
Embodiment
The utility model is described in further detail below in conjunction with the accompanying drawings, to make those skilled in the art with reference to explanation
Book word can be implemented according to this.
As illustrated in figs. 1 and 2, the utility model provides a kind of test strips for detecting canine parainfluenza virus antibody, including：Branch
Layer 1 is supportted, it includes multiple support blocks 11, and the side of each support block has inserting groove 13, and relative opposite side has plug division
12, the plug division of one of support block is inserted into the inserting groove of another support block in two neighboring support block, so that
Multiple support blocks are sequentially connected as an entirety；Detection layers, it includes the sample adsorption being set in turn on the supporting layer
Fibrous layer, gold mark fibrous layer, cellulose film layer and absorbent material layer, wherein, detection print is formed with the cellulose film layer
The restructuring of the canine parainfluenza virus or colloid gold label of colloid gold label is adsorbed with mark and control trace, the gold mark fibrous layer
Canine parainfluenza virus HN albumen, the detection trace is to be formed by the canine parainfluenza virus antigen liquid printing purified, the control
Trace is to be formed by sheep or the printing of rabbit-anti canine parainfluenza virus IgG solution.
Supporting layer 1 be refer in Fig. 1, figure by being made with plastic slice bar, sample adsorption fibrous layer 2 is with glass cotton
Into gold mark fibrous layer 3 is the restructuring canine parainfluenza virus of the canine parainfluenza virus or colloid gold label that are attached with colloid gold label
What the mineral wool of HN albumen was made, cellulose film layer 4 is what nitrocellulose filter was made, and absorbent material layer 5 is by absorbent filter
It is made, above layers is pasted onto on supporting layer 1 successively, the intersection fiber spliced each other crosses one another infiltration.In fiber
In plain film layer 4, with the canine parainfluenza virus antigen liquid mark detection trace 6 of purifying, code name T, with sheep or rabbit-anti dog parainfluenza virus
Malicious IgG solution mark control trace 7, code name C；Detection trace and the spread pattern for compareing trace are " | | ".
As shown in Fig. 2 the first protective layer 8 is to be covered in the test lead above sample adsorption fibrous layer 2 and gold mark fibrous layer 3
White diaphragm, marked in sample adsorption layer 2 and gold and be partial to sample adsorption on the corresponding white diaphragm of the intersection of fibrous layer 3
Trace line 9 is printed at the side 0.5cm of fibrous layer 2, the right-hand member of trace line is printed on arrow and MAX printed words, positioned at the water suction of handle end
Covered with other colors such as yellow diaphragm 10 on layer 5.
Fig. 3 is refer to, Fig. 3 provides the structural representation of supporting layer.Supporting layer of the present utility model includes multiple support blocks,
And each support block both sides relative to each other are respectively formed with a support in plug division and inserting groove, two neighboring support block
The plug division of block is plugged in the inserting groove of another support block, so as to realize the grafting of the two.When needing to change test strips
Length, it is necessary to change the length of supporting layer.By the number for increasing or decreasing support block, you can to realize to supporting layer length
Change.
When the length of supporting layer is determined, then detection layers are set on supporting layer, it is final obtain needed for length test paper
Bar.
The utility model is simple in construction, easy to use, with low cost, and Detection results are good.
Illustrate the detection operating method of the test strips below：
A detects the preparation of sample liquid：It is sterile to take the blood of tested animal, and serum is separated, make 1 with physiological saline:200 times
Dilute serum, obtain testing sample；
B detection operations：The test strips test lead is inserted in detected sample, insertion depth is no more than trace line, about 30
Test strips, horizontal positioned about 1-5 minutes are taken out after second, while observing result.
C results judge：If only showing a brownish red control trace C on the cellulose membrane of test strips, represent to survey
Inspection result is negative, and illustrates not detecting canine parainfluenza virus antibody in test sample liquid；If the cellulose in test strips
There is the control trace C of brownish red and detection trace T in film, represents that testing result is positive, i.e., dog pair is detected in measuring samples
Antibody of Influenza；If there is no any brownish red trace to show on cellulose membrane, show that test strips have failed or operated
By mistake.
The Cleaning Principle of test strips of the present invention is：
After test strips test lead is adsorbing fiber layer insertion detected sample solution, solution to be checked is by chromatographing zone of action
The gold mark antigen moved in canine parainfluenza virus antibody to be checked and gold mark tunica fibrosa spreads to cellulose membrane together, and eventually penetrates hand
In the water accepting layer of pommel, antiviral antibody to be checked can be corresponding with the antiviral antibody in diffusion process gold mark virus or golden indicated weight group
Albumen HN is combined, and then with detecting the virus combination in trace on cellulose membrane, so as to show the detection trace of brownish red
T；And compare the sheep in trace or rabbit-anti canine parainfluenza virus IgG then can mark canine recombinant sidestream with gold mark canine parainfluenza virus or gold
Influenza Virus VP1 protein bindings, form brownish red control trace C.If not having CPIV antibody in measuring samples liquid, test strips only show
One brownish red control trace C is shown；If there is no any brownish red trace to show on cellulose membrane, show that test strips have been lost
Effect or operational error.
In a preferred embodiment, in the test strips of described detection canine parainfluenza virus antibody, the sample is inhaled
Linking line between attached fibrous layer and the gold mark fibrous layer is fine with being located at the sample adsorption fibrous layer and the gold mark respectively
Tie up layer below two support blocks closing line alignment, it is described gold mark fibrous layer and the cellulose film layer between linking line with
It is located at the closing line alignment of two support blocks below the gold mark fibrous layer and the cellulose film layer, the cellulose respectively
Linking line and two branch respectively below the cellulose film layer and absorbent material layer between film layer and absorbent material layer
The closing line alignment of bracer.
That is, one or more complete support blocks are provided with below each layer.Such as, under sample adsorption fibrous layer
Fang Yousan support block, is arranged with a support block in gold mark fibrous layer, three is correspondingly arranged on below cellulose film layer
Individual support block, five support blocks are correspondingly arranged in absorbent material layer.When test is completed, can by stirring support block, and incite somebody to action
Sample adsorption fibrous layer, gold mark fibrous layer and absorbent material layer are removed, and only retain the middle cellulose with detection trace
Film layer.The utility model further increases test strips and is using upper flexibility and convenience.
In a preferred embodiment, in the test strips of described detection canine parainfluenza virus antibody, the supporting layer
By being made up of hard plastic bar.
In a preferred embodiment, in the test strips of described detection canine parainfluenza virus antibody, the sample is inhaled
Attached fibrous layer of mineral wool, nylon fiber or polyester fiber by being made up.
In a preferred embodiment, in the test strips of described detection canine parainfluenza virus antibody, the gold mark is fine
Layer is tieed up to be made up of mineral wool.
In a preferred embodiment, in the test strips of described detection canine parainfluenza virus antibody, the cellulose
Film layer of nitrocellulose filter, cellulose membrane, carboxymethyl cellulose film or polyvinylidene fluoride PVDF cellulose membranes by being made up.
In a preferred embodiment, in the test strips of described detection canine parainfluenza virus antibody, the water-absorption material
The bed of material is made of blotting paper.
In a preferred embodiment, in the test strips of described detection canine parainfluenza virus antibody, the sample is inhaled
Covered with the first protective layer on attached fibrous layer, the gold mark fibrous layer, covered with the second protective layer on the absorbent material layer,
Sample mark line is printed with first protective layer.
In a preferred embodiment, in the test strips of described detection canine parainfluenza virus antibody, when in the fibre
When a detection trace and a control trace are formed with the plain film layer of dimension, the arrangement of the detection trace and the control trace
Mode is | |, 10, ┬ ┬, ┴ ┴, ├ ├, ┤ ┤ and ● ● any of；It is formed with when in the cellulose film layer
When two detection traces and a control trace, two detection traces and a control trace are arranged in order, and arrangement mode is |
| |, 10, ┬ ┬ ┬, ┴ ┴ ┴, ├ ├ ├, ┤ ┤ ┤ and ● ● ● any of.
Although embodiment of the present utility model is disclosed as above, it is not restricted in specification and embodiment
Listed to use, it can be applied to various suitable fields of the present utility model completely, for those skilled in the art,
Other modification is easily achieved, therefore under the universal limited without departing substantially from claim and equivalency range, this reality
Specific details is not limited to new and shown here as the legend with description.
Claims (9)
1. a kind of test strips for detecting canine parainfluenza virus antibody, it is characterised in that including：
Supporting layer, it includes multiple support blocks, and the side of each support block has inserting groove, and relative opposite side has grafting
The plug division of one of support block is inserted into the inserting groove of another support block in portion, two neighboring support block, so that
Multiple support blocks are sequentially connected as an entirety；
Detection layers, it includes the sample adsorption fibrous layer, gold mark fibrous layer, cellulose film layer being set in turn on the supporting layer
And absorbent material layer, wherein, it is formed with the cellulose film layer on detection trace and control trace, the gold mark fibrous layer
It is adsorbed with the canine parainfluenza virus of colloid gold label or the restructuring canine parainfluenza virus HN albumen of colloid gold label, the detection print
Mark is to be formed by the canine parainfluenza virus antigen liquid printing purified, and the control trace is by sheep or rabbit-anti canine parainfluenza virus
The printing of IgG solution is formed.
2. the test strips of canine parainfluenza virus antibody are detected as claimed in claim 1, it is characterised in that the multiple support block
Including four support blocks, and the sample adsorption fibrous layer, gold mark fibrous layer, cellulose film layer and absorbent material layer are right successively
It should be arranged in each support block.
3. the test strips of canine parainfluenza virus antibody are detected as claimed in claim 1, it is characterised in that the supporting layer is served as reasons
Hard plastic bar is made.
4. the test strips of canine parainfluenza virus antibody are detected as claimed in claim 1, it is characterised in that the sample adsorption is fine
Layer is tieed up to be made up of mineral wool, nylon fiber or polyester fiber.
5. the test strips of canine parainfluenza virus antibody are detected as claimed in claim 1, it is characterised in that the gold mark fibrous layer
By being made up of mineral wool.
6. the test strips of canine parainfluenza virus antibody are detected as claimed in claim 1, it is characterised in that the cellulose film layer
By being made up of nitrocellulose filter, cellulose membrane, carboxymethyl cellulose film or polyvinylidene fluoride PVDF cellulose membranes.
7. the test strips of canine parainfluenza virus antibody are detected as claimed in claim 1, it is characterised in that the absorbent material layer
It is made of blotting paper.
8. the test strips of canine parainfluenza virus antibody are detected as claimed in claim 1, it is characterised in that the sample adsorption is fine
Covered with the first protective layer on dimension layer, the gold mark fibrous layer, covered with the second protective layer on the absorbent material layer, described
Sample mark line is printed with first protective layer.
9. the test strips of canine parainfluenza virus antibody are detected as claimed in claim 1, it is characterised in that when in the cellulose
When a detection trace and a control trace are formed with film layer, the arrangement mode of the detection trace and the control trace
For | |, 10, ┬ ┬, ┴ ┴, ├ ├, ┤ ┤ and ● ● any of；When being formed with two in the cellulose film layer
When detecting trace and a control trace, two detection traces and a control trace are arranged in order, and arrangement mode is | | |,
10, ┬ ┬ ┬, ┴ ┴ ┴, ├ ├ ├, ┤ ┤ ┤ and ● ● ● any of.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720266537.1U CN206573592U (en) | 2017-03-17 | 2017-03-17 | A kind of test strips for detecting canine parainfluenza virus antibody |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720266537.1U CN206573592U (en) | 2017-03-17 | 2017-03-17 | A kind of test strips for detecting canine parainfluenza virus antibody |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206573592U true CN206573592U (en) | 2017-10-20 |
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ID=60055324
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720266537.1U Expired - Fee Related CN206573592U (en) | 2017-03-17 | 2017-03-17 | A kind of test strips for detecting canine parainfluenza virus antibody |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206573592U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117224689A (en) * | 2023-11-16 | 2023-12-15 | 上海复宏汉霖生物技术股份有限公司 | Use of a combination of an anti-HER 2 antibody and a chemotherapeutic agent for the treatment of gastric cancer |
-
2017
- 2017-03-17 CN CN201720266537.1U patent/CN206573592U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117224689A (en) * | 2023-11-16 | 2023-12-15 | 上海复宏汉霖生物技术股份有限公司 | Use of a combination of an anti-HER 2 antibody and a chemotherapeutic agent for the treatment of gastric cancer |
| CN117224689B (en) * | 2023-11-16 | 2024-02-23 | 上海复宏汉霖生物技术股份有限公司 | Use of a combination of an anti-HER 2 antibody and a chemotherapeutic agent for the treatment of gastric cancer |
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|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20171020 Termination date: 20180317 |
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| CF01 | Termination of patent right due to non-payment of annual fee |