CN205539002U - Detect test paper strip of gosling pestivirus antibody - Google Patents
Detect test paper strip of gosling pestivirus antibody Download PDFInfo
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- CN205539002U CN205539002U CN201620159009.1U CN201620159009U CN205539002U CN 205539002 U CN205539002 U CN 205539002U CN 201620159009 U CN201620159009 U CN 201620159009U CN 205539002 U CN205539002 U CN 205539002U
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Abstract
The utility model provides a quick detect test paper strip of gosling pestivirus antibody, includes the supporting layer, and sample absorbent fibrous layer is pasted to supporting layer one end, and the layer that absorbs water is pasted to the other end, pastes cellulose membrane layer and gold mark fibrous layer, absorbent fibrous layer, gold mark fibrous layer, cellulose membrane layer, the juncture fibre alternately infiltration mutually that absorbs water and splice each other between the layer on the absorbent fibrous layer of supporting layer and the part that absorbs water between the layer. The utility model discloses at least, contain following beneficial effect: (1 ) the utility model discloses according to ELISA's basic principle, detect virus with the immunity chromatography test paper, utilize millipore filtration's filtration concentration and capillary, with antigen the antibody response goes on on going to the solid phase filter membrane to by traditional liquid phase environment of ELISA fast to adopt the colloidal gold trace to replace the enzyme trace, rely on the color development situation of naked eye direct observation colloidal gold, obtain the testing result immediately, thus this method is more more simple and convenient than serology methods such as ELISA, fast. (2 )The detection specificity is strong, and sensitiveness is high.
Description
Technical field
This utility model relates to a kind of utensil detecting Goose Parvovirus antibody, particularly relates to one and quickly detects gosling
The test strips of Pestivirus antibody.
Background technology
Goose Parvovirus (gosling plague virus, GPV), also known as goose parvovirus, can cause the one of young goose
Acute or subacute septicemia gosling plague (gosling plague, GP), to occur exudative enteritis to be main pathological change.
Primary disease mainly encroaches on 4-20 age in days young bird goose, infects fast, case fatality rate height.Primary disease the earliest by Chinese scholar Fang Dingyi in 1956 at me
State Operation in Yangzhou Area finds, most domestic supports goose provinces and regions all has generation, provisions goose industry to cause Important Economic to lose.
Quickly detection Goose Parvovirus antibody, is possible not only to evaluate immune effect of vaccine, and can diagnose with adjuvant clinical,
It is effectively to control the premise that Goose Parvovirus infects.The method of detection Goose Parvovirus antibody mainly has agar immunity to expand at present
Dissipate in test (AGP), serum and the method such as experiment, IFA and ELISA.But, AGP is the longest, in serum and experiment, IFA and
ELISA detection technique operation complexity, needs that expensive instrument and equipment, process be longer, time and effort consuming, need particular instrument equipment and
Professional and technical personnel operates, and is difficult to popularize in basic unit, it is impossible to meet production line or epidemic disease on-the-spot quickly detect needs.Cause
This, it would be highly desirable to there are equipment and the technology of a kind of quick detection Goose Parvovirus antibody.
Utility model content
A purpose of the present utility model is to provide the test strips of a kind of quick detection Goose Parvovirus antibody, and provides extremely
The advantage that will be described later less.
This utility model is it is also an object that provide a kind of result intuitive display, examination accurate, quickly detection GPV antibody
Paper slip, compared with other detection methods, this test strips high specificity, highly sensitive, easy and simple to handle, result show quickly, detection
With low cost.
The technical solution of the utility model particularly as follows:
The test strips of a kind of quick detection Goose Parvovirus antibody, including supporting layer 1, supporting layer 1 one end is pasted sample and is inhaled
Attached fibrous layer 2, the other end pastes water accepting layer 5, the bonding partially fiber between the adsorbing fiber layer 2 and water accepting layer 5 of supporting layer 1
Element film layer 4 and gold mark fibrous layer 3, adsorbing fiber layer 2, gold mark splice between fibrous layer 3, cellulose membrane layer 4, water accepting layer 5 each other
Intersection fiber cross one another infiltration, on described cellulose membrane layer 4, labelling is with the detection of the Goose Parvovirus antigen body of purification
Trace 6 with comprise sheep or rabbit anti gosling plague virus antibody compare trace 7, detection trace compares trace 7 arranged in parallel at fibre with 6
On dimension element film layer, that detects trace 6 is located proximate to adsorbing fiber layer；Gold mark fibrous layer 3 is the gosling being attached with colloid gold label
The glass cotton of the restructuring Goose Parvovirus VP3 albumen of Pestivirus or colloid gold label is made；It is fine that this test strips is provided with sample adsorption
One end of dimension layer 2 is holding end, and the one end being provided with water accepting layer is water intake end, and water intake end outer side covers has the first protecting film of white
81, holding end is coated with the second protecting film of yellow；In the protection that sample adsorption fibrous layer is corresponding with gold mark fibrous layer 3 intersection
It is partial on film at sample adsorption fibrous layer side 0.40cm be printed on trace line；Holding end is provided with the projection being easy to grip, protruding
Being marked with thumb shape, projection is elastomeric material.
Supporting layer is set to latticed at water intake end, generally circular in shape, square, triangle, the rhombus of its mesh, six limits
Shape.
Described supporting layer is for make with plastic slice bar.
This utility model at least includes following beneficial effect:
(1) detection high specificity, sensitivity is high.The test strips that this utility model provides is with colloid gold label Goose Parvovirus
Or be prepared from based on colloid gold label restructuring Goose Parvovirus VP3 albumen, gold grain and virus egg in gold secondary disease toxalbumin
Being formed without covalent bond between white molecule, the two is combined by the Van der Waals force between the charges of different polarity, and gold colloidal mark is to virus protein
Specificity and affinity impact the least, and there is higher trace rate.
(2) easy and simple to handle, quickly.Test strips of the present utility model is in use without additional any Other Instruments and examination
Agent, as long as by its test lead insertion measuring samples liquid about 30 seconds, i.e. can determine that testing result in about 5 minutes.
(3) result intuitive display, accurately.Test strips of the present utility model is to show henna detection trace and comparison print
Mark, as the positive detected and negative trace, i.e. shows two brownish red traces on cellulose membrane, represents at detected sample
In have antiviral antibody to detect, result is positive；On cellulose membrane, only one brownish red of display compares trace C, represents tested
Surveying in sample liquid and do not detect antiviral antibody, result is negative.Result judges directly perceived, accurate, simple and clear, is difficult to false negative occur
Judge by accident with false positive.
(4) investment and testing cost are reduced.Test strips of the present utility model in use, is not required to separately join Other Instruments, set
Standby and reagent, saves big measuring appratus, equipment and additive reagent expense；Specialty and layman all can carry out scene whenever and wherever possible
Detection, it is not necessary to payment expert diagnosis Laboratory Fee or feeding sample remove the travelling expenses of diagnosis room, saves testing cost, and testing cost is low.
(5) applied range, it is simple to popularity is applied.The test strips that this utility model provides is simple to operate, becomes " a step
Formula " or " foolproof ", and be convenient for carrying and preserve, the needs of different levels personnel can be met, including specialty chemical examination, customs's inspection
Epidemic disease, health and epidemic prevention, quality-monitoring, livestock products processing, intensive culture, to individuality cultivation etc., have wide market prospect and relatively
Good economical, societal benefits.
(6) this utility model is according to the ultimate principle of ELISA, detects antiviral antibody with immune chromatography test paper, utilizes micropore
The diafiltration concentration of filter membrane and capillarity, forwarded to antigen-antibody reaction on solid phase filter membrane by the Traditional liquid phase environment of ELISA
Quickly carry out, and use gold colloidal trace to replace enzyme trace, with the colour developing situation of direct visual perception gold colloidal, immediately obtain inspection
Surveying result, therefore the method is easier than serological methods such as ELISA, quickly.
Accompanying drawing explanation
Fig. 1 is generalized section of the present utility model；
Fig. 2 is schematic top plan view of the present utility model；
Fig. 3 is the schematic diagram of supporting layer.
Detailed description of the invention
Below in conjunction with the accompanying drawings this utility model is described in further detail, to make those skilled in the art with reference to explanation
Book word can be implemented according to this.
Such as Fig. 1 and Fig. 2, the test strips of a kind of quick detection Goose Parvovirus antibody, including supporting layer 1, supporting layer 1 one end
Pasting sample adsorption fibrous layer 2, the other end pastes water accepting layer 5, the portion between the adsorbing fiber layer 2 and water accepting layer 5 of supporting layer 1
Point paste cellulose membrane layer 4 and gold mark fibrous layer 3, adsorbing fiber layer 2, gold mark fibrous layer 3, cellulose membrane layer 4, water accepting layer 5 it
Between the intersection fiber that splices each other cross one another infiltration, on cellulose membrane layer 4, labelling is with the Goose Parvovirus antigen of purification
The detection trace 6 of body with comprise sheep or rabbit anti gosling plague virus antibody compare trace 7, detection trace is with 6 to compare traces 7 arranged side by side
Being arranged on cellulose membrane layer, that detects trace 6 is located proximate to adsorbing fiber layer, and the code name of detection trace 6 is T；Comparison trace 7
Code name be C.
Gold mark fibrous layer 3 is the restructuring Goose Parvovirus of Goose Parvovirus or the colloid gold label being attached with colloid gold label
The glass cotton of VP3 albumen is made.
It is holding end that this test strips is provided with one end of sample adsorption fibrous layer 2, and the one end being provided with water accepting layer 5 is water intake end
(also referred to as test lead), water intake end outer side covers has the first protecting film 81 of white, and holding end is coated with the second protection of yellow
Film 82.Sample adsorption fibrous layer 2 is partial to by the protecting film that sample adsorption fibrous layer 2 is corresponding with gold mark fibrous layer 3 intersection
Being printed on trace line 9 at the 0.40cm of side, the right-hand member of trace line is printed on arrow and MAX printed words.
Even if it is true that be provided with the protecting film of different colours, it is also possible to make testing staff be mixed with holding end by water intake end
Confuse, cause detection accident.So, the groove 83 or projection (only having drawn groove in figure) being easy to grip it is provided with at holding end, recessed
Groove 83 or raised marking have thumb shape, so arrange, and testing staff can be facilitated to distinguish water end (W.E.) and holding end, be also convenient for
Grip, more meet ergonomics.
If groove 83, groove 83 extends inward into water accepting layer 5 or supporting layer 1, convenient gripping.
If projection, projection can be elastomeric material, convenient gripping.
Supporting layer 1 is for make with plastic slice bar.
Such as Fig. 3, supporting layer 1 is set to latticed at water intake end, generally circular in shape, square, the triangle of its mesh 11, Pedicellus et Pericarpium Trapae
Shape, hexagon.During normal ELISA test strip, the opposite side of water accepting layer 5 only supporting layer 1 can absorb water (solution), and this reality
It is latticed with the water intake end of novel supporting layer 1, water accepting layer 5 can be made also to be inhaled by mesh 11 in the side of supporting layer 1
Water, so since, water accepting layer 5 can absorb water with two sides, accelerates the speed of water suction, shortens the time used by detection, improves
Work efficiency.
Sample adsorption fibrous layer 2 is for make with glass cotton.
Cellulose membrane layer 4 is made for nitrocellulose filter.
Water accepting layer 5 is for be made up of absorbent filter.
The detection operational approach of described test strips
The preparation of a detection sample liquid: the aseptic blood taking tested animal, and separate serum, make 1:200 with normal saline
Dilute serum again, obtains testing sample；
B detects operation: inserted by this test strips test lead in detected sample, and insertion depth is less than trace line, and about 30
Test strips, horizontal positioned about 1-5 minute, observed result simultaneously is taken out after Miao.
C result judges: if only demonstrating a brownish red comparison trace C on the cellulose membrane of test strips, representing and surveying
Inspection result is negative, and illustrates not detect Goose Parvovirus antibody in test sample liquid；If the cellulose membrane in test strips
Henna comparison trace C and detection trace T occurs, represents that testing result is positive, in measuring samples, i.e. detect gosling plague
Antiviral antibody；If not having any brownish red trace to show on cellulose membrane, then show that test strips had lost efficacy or operated wrong.
The Cleaning Principle of above-mentioned test strips:
After the i.e. adsorbing fiber layer of test strips test lead inserts detected sample solution, solution to be checked is by chromatography zone of action
Gold mark antigen in dynamic sick Goose Parvovirus antibody to be checked and gold mark fibrous membrane spreads to cellulose membrane together, and eventually penetrates hands
In the water accepting layer of pommel, the golden secondary disease that in diffusion process, antiviral antibody to be checked can be corresponding with this antiviral antibody is malicious or golden indicated weight group
Albumen VP3 combines, and then detects the virus combination in trace on cellulose membrane, thus demonstrates henna detection trace
T；Compare the sheep in trace or rabbit anti gosling plague virus IgG then can be with gold mark Goose Parvovirus or gold indicated weight group gosling blast
Poison VP3 protein binding, forms brownish red comparison trace C.If not having GPV antibody in measuring samples liquid, test strips only demonstrates
Article one, brownish red comparison trace C；If not having any brownish red trace to show on cellulose membrane, then show test strips lost efficacy or
Operational error.
Claims (2)
1. detecting a test strips for Goose Parvovirus antibody, including supporting layer (1), sample adsorption is pasted in supporting layer (1) one end
Fibrous layer (2), the other end pastes water accepting layer (5), the part between the adsorbing fiber layer (2) and water accepting layer (5) of supporting layer (1)
Paste cellulose membrane layer (4) and gold mark fibrous layer (3), adsorbing fiber layer (2), gold mark fibrous layer (3), cellulose membrane layer (4), suction
The intersection fiber spliced each other between water layer (5) crosses one another infiltration, it is characterised in that: described cellulose membrane layer (4) subscript
Note have the detection trace (6) of the Goose Parvovirus antigen body with purification with comprise sheep or rabbit anti gosling plague virus antibody compare print
Mark (7), detection trace (6) with compare trace (7) arranged in parallel on cellulose membrane layer, that detects trace (6) is located proximate to suction
Attached fibrous layer；
Gold mark fibrous layer (3) is for be attached with the Goose Parvovirus of colloid gold label or the restructuring Goose Parvovirus of colloid gold label
The glass cotton of VP3 albumen is made；
It is holding end that this test strips is provided with one end of sample adsorption fibrous layer (2), and the one end being provided with water accepting layer (5) is water intake end,
Water intake end outer side covers has first protecting film (81) of white, and holding end is coated with second protecting film (82) of yellow；At sample
It is partial to sample adsorption fibrous layer (2) side on the protecting film that adsorbing fiber layer (2) is corresponding with gold mark fibrous layer (3) intersection
Trace line (9) it is printed at 0.40cm；
Holding end is provided with the projection being easy to grip, and raised marking has thumb shape, and projection is elastomeric material；
Supporting layer (1) is set to latticed at water intake end, its mesh (11) generally circular in shape.
2. the test strips detecting Goose Parvovirus antibody as claimed in claim 1, it is characterised in that described supporting layer (1) is
Make with plastic slice bar.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620159009.1U CN205539002U (en) | 2016-03-02 | 2016-03-02 | Detect test paper strip of gosling pestivirus antibody |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620159009.1U CN205539002U (en) | 2016-03-02 | 2016-03-02 | Detect test paper strip of gosling pestivirus antibody |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205539002U true CN205539002U (en) | 2016-08-31 |
Family
ID=56779502
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620159009.1U Expired - Fee Related CN205539002U (en) | 2016-03-02 | 2016-03-02 | Detect test paper strip of gosling pestivirus antibody |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205539002U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106771180A (en) * | 2016-11-23 | 2017-05-31 | 百奥森（江苏）食品安全科技有限公司 | A kind of test strips for detecting Goose Parvovirus antibody |
-
2016
- 2016-03-02 CN CN201620159009.1U patent/CN205539002U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106771180A (en) * | 2016-11-23 | 2017-05-31 | 百奥森（江苏）食品安全科技有限公司 | A kind of test strips for detecting Goose Parvovirus antibody |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160831 Termination date: 20170302 |