CN206573590U - A kind of fluorescence immune chromatography instrument Quality Control detection card and detection suit - Google Patents
A kind of fluorescence immune chromatography instrument Quality Control detection card and detection suit Download PDFInfo
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- CN206573590U CN206573590U CN201621489364.1U CN201621489364U CN206573590U CN 206573590 U CN206573590 U CN 206573590U CN 201621489364 U CN201621489364 U CN 201621489364U CN 206573590 U CN206573590 U CN 206573590U
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Abstract
The utility model is related to immunochromatography detection field, and in particular to a kind of fluorescence immune chromatography instrument Quality Control detection card and detection suit.Quality Control detection card is on PVC bottom plates, to offer and cellulose nitrate film is fixed with detection window, detection window, and cellulose nitrate film is provided with a QCLine and a QTLine, QCLine and QTLine has drawn fluorescent microsphere solution.Choose at least 2 Quality Control detection cards and be combined into Quality Control detection suit.The Q of every Quality Control detection card is calculated successivelyT/QCArea ratio, and contrasted with standard curve, then judge that the measurement result reliability of instrument is low when error is higher than certain value.The utility model is detected applied to the Quality Control of fluorescence immune chromatography instrument, simple to operate, convenient, can be applied to Site Detection；Carry out instrument quality inspection first before being detected to sample, the reliability of sample measurement can be lifted.
Description
Technical field
The utility model is related to immunochromatography detection field, and in particular to a kind of fluorescence immune chromatography instrument Quality Control detection card
With detection suit.
Background technology
In the detection of biological and medical science, immunochromatography technique is often used.Immunochromatography technique refers to a kind of unique be immunized
Analysis mode.Its principle is a certain zone that special antibody is first fixed on to nitrocellulose filter, when the nitric acid of the drying is fine
After the plain one end immersion sample (urine or serum) of dimension, by capillarity make sample solution along the film on chromatography strip forward
It is mobile, when being moved to the region of the acceptor for being fixed with determinand (such as antibody or antigen), in sample corresponding determinand be with
This receptor is specifically bound, if with immune colloid gold or Immunoperoxidase Staining the region can be made to show certain color, so that
Realize specific immunodiagnosis.
Compared to other immunoassay methods, equipment that immunochromatographic method need not be expensive, professional operator and
Complicated operating procedure can just be quickly obtained testing result.Current China rhythm of life is more and more faster, various infectious diseases
The incidence of disease constantly rises, and the living safety to the people brings great potential safety hazard.And the product based on immunochromatography technique because
It is simple operation, quick and only need to naked eyes and can be obtained by final result, can as a kind of disease detection and prevention
Instrument.Developing such product is conducive to China to alleviate the situation that common transmittable disease incidence increasingly rises.
Yet with the interference of the factor and external environment of instrument in itself, the result of measurement has certain probability to produce
The situation of mistaken diagnosis.In order to exclude this case, except lifting the manufacture craft of instrument and the processing level of the data collected,
Quality inspection can also be carried out to instrument before using instrument.
Utility model content
The purpose of this utility model is that there is provided a kind of fluorescence immune chromatography instrument Quality Control for the above-mentioned the deficiencies in the prior art of solution
Detection card and detection suit.
The utility model is achieved through the following technical solutions：
A kind of fluorescence immune chromatography instrument Quality Control detection card, on PVC bottom plates, is offered in detection window, detection window
Cellulose nitrate film is fixed with, cellulose nitrate film is provided with a QCLine and a QTLine, the QCLine and QTLine is drawn
There is fluorescent microsphere solution.
A kind of fluorescence immune chromatography instrument Quality Control detection suit, the Quality Control detection suit includes at least 2 Quality Control detections
Card, every upper Q of Quality Control detection cardTThe fluorescent microsphere solution concentration of line is different, and between corresponding to instrument minimum sensitivity
Between concentration corresponding to concentration and apparatus measures dynamic range maximum, every upper Q of Quality Control detection cardCThe fluorescent microsphere of line is molten
Liquid concentration all same, and it is same right between the concentration corresponding to instrument minimum sensitivity and apparatus measures dynamic range maximum institute
Between the concentration answered.
Preferably, 1 Q is contained in the Quality Control detection suitTThe fluorescent microsphere solution concentration of line is that instrument minimum is sensitive
The Quality Control detection card and 1 Q of the corresponding concentration of degreeTThe fluorescent microsphere solution concentration of line is apparatus measures dynamic range maximum institute
The Quality Control detection card of corresponding concentration, 2 upper Q of Quality Control detection cardCThe fluorescent microsphere solution concentration of line is identical and minimum between instrument
Between the concentration corresponding to concentration and apparatus measures dynamic range maximum corresponding to sensitivity.
Preferably, the fluorescent microsphere is surface without one in modification group or surface carboxyl functionalized fluorescent microsphere
Kind.
The beneficial effects of the utility model are：
1st, the utility model is detected applied to the Quality Control of fluorescence immune chromatography instrument, simple to operate, convenient, can be applied to scene
Detection；
2nd, the utility model product is easy to preserve, and is convenient for carrying, hot-swappable, at any time the reliability of detecting instrument；
3rd, the utility model is label, fluorescent stabilization from fluorescent microsphere；
4th, carry out instrument quality inspection first before being detected to sample, the reliability of sample measurement can be lifted.
Brief description of the drawings
Fig. 1 is the structural representation of fluorescence immune chromatography instrument Quality Control detection card；
Fig. 2 is the Making programme figure of fluorescence immune chromatography instrument Quality Control detection card.
Reference：
1, PVC bottom plate；2, cellulose nitrate film；3, detection window；4, QCLine；5, QTLine.
Embodiment
To be best understood from the utility model, the utility model is further described with reference to embodiment and accompanying drawing, with
Lower embodiment is only that the utility model is illustrated rather than it is limited.
As shown in figure 1, a kind of fluorescence immune chromatography instrument Quality Control detection card, on PVC bottom plates 1, offers detection window
3, cellulose nitrate film 2 is fixed with detection window 3, cellulose nitrate film 2 is provided with a QCLine 4 and a QTLine 5,
The QCLine 4 and QTLine 5 has drawn fluorescent microsphere solution.
As shown in Fig. 2 the preparation method of fluorescence immune chromatography instrument Quality Control detection card is as follows：
1. solution allocation
A. BANGS microballoon stostes that 10 μ L concentration are 10mg/mL are measured with pipettor into centrifuge tube.
B. plus the μ L of PBS dilutions 990 are into 10 μ L microspheres solutions, mixed liquor is placed in solution rapid mixer vortex,
Switch is opened, quick be vortexed is carried out and mixes 2min.Solution for later use after mixing；(microspheres solution for forming 100 μ g/mL)
C. the μ L of microspheres solution 500 of 100 μ g/mL of generation in (b) are taken, the μ L of PBS dilutions 500 is added, mixed liquor is put
In in solution rapid mixer vortex, switch is opened, quick be vortexed is carried out and mixes 2min.Solution for later use after mixing；(form 50 μ
G/mL microspheres solution)
D. the μ L of microspheres solution 500 of 50 μ g/mL of generation in (c) are taken, the μ L of PBS dilutions 500 is added, mixed liquor is placed in
In solution rapid mixer vortex, switch is opened, quick be vortexed is carried out and mixes 2min.Solution for later use after mixing；(form 25 μ g/
ML microspheres solution)
E. the μ L of microspheres solution 500 of 25 μ g/mL of generation in (d) are taken, the μ L of PBS dilutions 500 is added, mixed liquor is placed in
In solution rapid mixer vortex, switch is opened, quick be vortexed is carried out and mixes 2min.Solution for later use after mixing；(form 12.5 μ
G/mL microspheres solution)
F. the μ L of microspheres solution 500 of 12.5 μ g/mL of generation in (e) are taken, the μ L of PBS dilutions 500 is added, mixed liquor is put
In in solution rapid mixer vortex, switch is opened, quick be vortexed is carried out and mixes 2min.Solution for later use after mixing；(form 6.25
μ g/mL microspheres solution)
G. the μ L of microspheres solution 500 of 6.25 μ g/mL of generation in (f) are taken, the μ L of PBS dilutions 500 is added, mixed liquor is put
In in solution rapid mixer vortex, switch is opened, quick be vortexed is carried out and mixes 2min.Solution for later use after mixing；(formed
3.125 μ g/mL microspheres solution)
H. the μ L of microspheres solution 10 of 100 μ g/mL of generation in (1) are taken, the μ L of PBS dilutions 990 is added, mixed liquor is placed in
In solution rapid mixer vortex, switch is opened, quick be vortexed is carried out and mixes 2min.Solution for later use after mixing；(form 1 μ g/mL
Microspheres solution)
2. line
A. cellulose nitrate film is taken, is pasted onto on PVC backboards, the relevant position of spray film lining instrument is placed on, it is stand-by；
B. two pipelines 3 times of spray film line instrument are cleaned with pure water；
C. conduit positions are adjusted, make two pipeline interval 6mm；
D. stroked parameters are set to：Rule speed 50mm/s, the μ L/cm of line amount 1；
E. the microspheres solution prepared is added in two pipelines with spraying lining instrument；
F. the microspheres solution line liquid (Q that distal line addition solution concentration is 25 μ g/mLCLine), it is 100 μ that near-end, which draws concentration,
G/mL microspheres solutions (QTLine).Start button is clicked on, instrument starts to rule on nitrocellulose filter, and line is finished, and pure water is clear
Wash pipeline 3 times, wait next concentration spray film line；
G. the microspheres solution line liquid (Q that distal line addition solution concentration is 25 μ g/mLCLine), it is 50 μ g/ that near-end, which draws concentration,
ML microspheres solutions (QTLine)；Start button is clicked on, instrument starts to rule on nitrocellulose filter, and line is finished, pure water cleaning
Pipeline 3 times, waits next concentration spray film line；
H. the microspheres solution line liquid (Q that distal line addition solution concentration is 25 μ g/mLCLine), it is 25 μ g/ that near-end, which draws concentration,
ML microspheres solutions (QTLine).Start button is clicked on, instrument starts to rule on nitrocellulose filter, and line is finished, pure water cleaning
Pipeline 3 times, waits next concentration spray film line；
I. the microspheres solution line liquid (Q that distal line addition solution concentration is 25 μ g/mLCLine), it is 12.5 μ that near-end, which draws concentration,
G/mL microspheres solutions (QTLine).Start button is clicked on, instrument starts to rule on nitrocellulose filter, and line is finished, and pure water is clear
Wash pipeline 3 times, wait next concentration spray film line；
J. the microspheres solution line liquid (Q that distal line addition solution concentration is 25 μ g/mLCLine), it is 6.25 μ that near-end, which draws concentration,
G/mL microspheres solutions (QTLine).Start button is clicked on, instrument starts to rule on nitrocellulose filter, and line is finished, and pure water is clear
Wash pipeline 3 times, wait next concentration spray film line；
K. the microspheres solution line liquid (Q that distal line addition solution concentration is 25 μ g/mLCLine), it is 3.125 that near-end, which draws concentration,
μ g/mL microspheres solutions (QTLine).Start button is clicked on, instrument starts to rule on nitrocellulose filter, and line is finished, and pure water is clear
Wash pipeline 3 times, wait next concentration spray film line；
L. the microspheres solution line liquid (Q that distal line addition solution concentration is 25 μ g/mLCLine), it is 1 μ g/ that near-end, which draws concentration,
ML microspheres solutions (QTLine).Start button is clicked on, instrument starts to rule on nitrocellulose filter, and line is finished, pure water cleaning
Pipeline 3 times, closes instrument.
3. film of ruling is dried
The nitrocellulose filter for completion of ruling is put in drying room and dried 1 hour, 37 DEG C of drying temperature.At interval of 15min notes
Record temperature in a drying room.
4. slitting
Take it is dried after line PVC bottom plates, be put into cutting machine, it is 4mm to set slitting width, click is proceeded by and cut
Bar.
5. preserve
The test strips cut are collected in aluminium foil bag respectively by concentration, is put into after drier and seals preservation.When need to use
Concentration test strips needed for choosing are placed in inside reagent strip cartridge neck, and instrument detection is can be used to after pressure shell.Detection after pressure shell
Card need to be placed in drying box and preserve, and its humidity should be controlled below 30%.
6. detection
Above-mentioned detection card is sequentially placed into detection window, the Q of every Quality Control bar is calculated successivelyT/QCArea ratio, and with mark
Directrix curve is contrasted；As a result it is as shown in the table：
By contrasting two groups of data, the error that can obtain two groups of data is respectively：4.5%, 3.44%, 1.2%,
1.36%, 1.19%, 5.06%, 0.0363%.
Error is below 6%, it was demonstrated that now such as measured with instrument, then measurement result is reliable.
Embodiment described above is only that preferred embodiment of the present utility model is described, not to this practicality
New scope is defined, on the premise of the utility model design spirit is not departed from, and those of ordinary skill in the art are to this
Various modifications and improvement that the technical scheme of utility model is made, all should fall into the guarantor that claims of the present utility model are determined
In the range of shield.
Claims (4)
1. a kind of fluorescence immune chromatography instrument Quality Control detection card, it is characterised in that：On PVC bottom plates (1), detection window is offered
(3) cellulose nitrate film (2), is fixed with detection window (3), cellulose nitrate film (2) is provided with a QCLine (4) and
One QTLine (5), the QCLine (4) and QTLine (5) has drawn fluorescent microsphere solution.
2. a kind of detection suit of the fluorescence immune chromatography instrument Quality Control detection card described in utilization claim 1, it is characterised in that：
The Quality Control detection suit includes at least 2 Quality Control detection cards, every upper Q of Quality Control detection cardTThe fluorescent microsphere solution concentration of line
It is different, and between the concentration corresponding to the concentration corresponding to instrument minimum sensitivity and apparatus measures dynamic range maximum it
Between, every upper Q of Quality Control detection cardCThe fluorescent microsphere solution concentration all same of line, and it is same right between instrument minimum sensitivity institute
Between the concentration corresponding to concentration and apparatus measures dynamic range maximum answered.
3. a kind of detection suit of utilization fluorescence immune chromatography instrument Quality Control detection card according to claim 2, its feature
It is：Contain 1 Q in the Quality Control detection suitTThe fluorescent microsphere solution concentration of line is dense corresponding to instrument minimum sensitivity
The Quality Control detection card of degree and 1 QTThe fluorescent microsphere solution concentration of line is concentration corresponding to apparatus measures dynamic range maximum
Quality Control detection card, 2 upper Q of Quality Control detection cardCThe fluorescent microsphere solution concentration of line is identical and right between instrument minimum sensitivity institute
Between the concentration corresponding to concentration and apparatus measures dynamic range maximum answered.
4. a kind of detection suit of utilization fluorescence immune chromatography instrument Quality Control detection card according to claim 2, its feature
It is：The fluorescent microsphere is surface without one kind in modification group or surface carboxyl functionalized fluorescent microsphere.
Priority Applications (1)
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| CN201621489364.1U CN206573590U (en) | 2016-12-31 | 2016-12-31 | A kind of fluorescence immune chromatography instrument Quality Control detection card and detection suit |
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| CN201621489364.1U CN206573590U (en) | 2016-12-31 | 2016-12-31 | A kind of fluorescence immune chromatography instrument Quality Control detection card and detection suit |
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| CN206573590U true CN206573590U (en) | 2017-10-20 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107037215A (en) * | 2016-12-31 | 2017-08-11 | 必欧瀚生物技术（合肥）有限公司 | A kind of fluorescence immune chromatography instrument Quality Control detection card, detection suit, its preparation method and detection method |
-
2016
- 2016-12-31 CN CN201621489364.1U patent/CN206573590U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107037215A (en) * | 2016-12-31 | 2017-08-11 | 必欧瀚生物技术（合肥）有限公司 | A kind of fluorescence immune chromatography instrument Quality Control detection card, detection suit, its preparation method and detection method |
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| GR01 | Patent grant | ||
| CP02 | Change in the address of a patent holder |
Address after: 230088 biohan biological industrial park, northeast corner of the intersection of kongtai road and Chang'an Road, high tech Zone, Hefei, Anhui Province Patentee after: BIOHIT BIOTECHNOLOGY (HEFEI) Co.,Ltd. Address before: 230088 first to fourth floors, D9 building, Hefei Innovation Industrial Park, 800 Wangjiang West Road, high tech Zone, Hefei, Anhui Province Patentee before: BIOHIT BIOTECHNOLOGY (HEFEI) Co.,Ltd. |
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