CN206399840U - Detect the Rapid detection test strip of Organophosphorus and carbamate pesticides pesticide residue - Google Patents
Detect the Rapid detection test strip of Organophosphorus and carbamate pesticides pesticide residue Download PDFInfo
- Publication number
- CN206399840U CN206399840U CN201621203784.9U CN201621203784U CN206399840U CN 206399840 U CN206399840 U CN 206399840U CN 201621203784 U CN201621203784 U CN 201621203784U CN 206399840 U CN206399840 U CN 206399840U
- Authority
- CN
- China
- Prior art keywords
- detection
- nitrocellulose filter
- back board
- supporting back
- organophosphorus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
The utility model discloses a kind of Rapid detection test strip for detecting Organophosphorus and carbamate pesticides pesticide residue, including supporting back board and the detection film being attached in supporting back board, detection film includes the nitrocellulose filter which is provided with detection band, the absorbing membrane mutually overlapped with nitrocellulose filter is pasted with the supporting back board on the left of nitrocellulose filter, overlap joint is provided with substrate pad, enzyme piece and sample pad successively in the supporting back board on the right side of nitrocellulose filter.The utility model has the advantage of make the Rapid detection test strip for Organophosphorus and carbamate pesticides pesticide residue using coupling reaction principle first, reagent strip is simple in construction, make quick, it is easy to operate, specialized instrument and equipment is not needed, without reagent preparation, testing staff is not required to training, show the presence of agricultural chemicals is whether there is in sample indirectly by observing the color of detection band, very intuitively.
Description
Technical field
The utility model is related to biological technical field, and Organophosphorus and carbamate pesticides class is detected more particularly, to a kind of wide spectrum
The Rapid detection test strip of residues of pesticides.
Background technology
Agricultural chemicals has been increasingly becoming an important step of agricultural production as the important tool of preventing and treating pest and disease damage.But go out
Some leaks when supervising and using, Pesticide Residue has been increasingly becoming one of obstacle of modern agricultural development.It is organic
It is Phosphorus the advantages of agricultural chemicals is with its wide spectrum, efficient, degradable, half-life short is killed with carbamates to be widely used in modern agriculture
In, to the mankind with huge economic benefit, but the problems such as environment and food pollution is also generated simultaneously.Food security in recent years
Major event layer goes out constantly, causes people generally to be held the suspicious attitude for domestic food security, how to spend this and " trusts danger
Machine " is the problem of China's food service industry is anxious to be resolved.
The detection method of existing agricultural chemicals mainly includes instrument detection and quick detection, and the detection of its Instrumental mainly includes gas phase
All multiple instruments detection methods including chromatography, high performance liquid chromatography, gas chromatography and mass spectromentry GC-MS etc.；Quick detection
Mainly include colorimetric method, enzyme piece method and hexavalent chrome bio-removal.When detecting vegetable pesticide residue using instrument, because to testing staff's element
Matter requires height, equipment costliness, check analysis cycle length, the features such as Expenses of laboratory examination is more, it is difficult to realize the quick detection in market, can be with
Used as the final judgement detection method of testing result；Fast detection method is easy to operate, it is not necessary to which instrument and equipment and preparation are tried
Agent, testing staff is not required to training, is that market, station, small towns etc. require detection sensitivity most preferably to examine used in relatively low field
Survey method.The existing residual rapid method of agriculture is compared with traditional chromatography, spectroscopic methodology, although being more suitable for a large amount of sample screenings and existing
Field detection, but have the disadvantage the degree of accuracy and sensitivity shortcoming.In order to ensure the quality safety of food, promote the external of agricultural products in China
The export trade, studying effective rapid detection for pesticide residue analysis method has highly important meaning.
As can be seen that existing food quality supervision system is generally built upon on the basis of Laboratory Instruments analytical technology,
The sampling inspection process of food, not only time and effort consuming, and information issue is serious delayed, it is very difficult to carried out to the product that consumer queries
Fast reaction.Simultaneously, because the distrust to Supervision, increasing consumer is desirable to oneself and product is carried out quickly
Qualitative detection, especially to fruit and vegetable necessary to daily life.Therefore, it is quick, sensitive, inexpensive in the urgent need to developing
Food security scene speed up measure method.
The content of the invention
The purpose of this utility model be to provide a kind of highly sensitive, inexpensive, easy-operating disposable detection organophosphor and
The Rapid detection test strip of carbamate pesticide residue.
To achieve the above object, the utility model can take following technical proposals：
The Rapid detection test strip of detection Organophosphorus and carbamate pesticides pesticide residue described in the utility model, including
Supporting back board and the detection film being attached in the supporting back board, the detection film include the cellulose nitrate which is provided with detection band
Plain film, is pasted with the water suction mutually overlapped with the nitrocellulose filter in the supporting back board on the left of the nitrocellulose filter
Film, overlap joint is provided with substrate pad, enzyme piece and sample pad successively in the supporting back board on the right side of the nitrocellulose filter.
The supporting back board is the polyvinyl chloride plastic sheets that thickness is 0.3 ~ 0.7mm, and its length is 60 ~ 100mm, width
3~5mm。
The sample pad is 0.4 ~ 0.7mm of thickness, long 10 ~ 20mm, wide 3 ~ 5mm blotting paper；The enzyme piece is immersed with second
0.2 ~ 0.5mm of thickness of acetylcholinesterase, long 10 ~ 20mm, wide 3 ~ 5mm water adsorption glass tunica fibrosa；The substrate pad be immersed with
0.3 ~ 0.6mm of thickness of ethychlozate ester, long 10 ~ 20mm, wide 3 ~ 5mm water imbibition polyester fiber film；The thickness 0.3 of the absorbing membrane
~ 0.5mm, long 10 ~ 20mm, wide 3 ~ 5mm.
The nitrocellulose filter is NC films, and 10 ~ 20mm of its length, wide 3 ~ 5mm, the detection band are to draw in the nitric acid
Fast red or solid blue reagent line on cellulose membrane.
The lap of splice between adjacent two diaphragm is 1 ~ 2mm.
The utility model has the advantage of made first using coupling reaction principle for Organophosphorus and carbamate pesticides class
The Rapid detection test strip of residues of pesticides, reagent strip is simple in construction, makes quick, easy to operate, it is not necessary to specialized instrument and equipment,
Without reagent preparation, testing staff is not required to training, shows agricultural chemicals is whether there is in sample indirectly by observing the color of detection band
In the presence of very intuitively.
Brief description of the drawings
Fig. 1 is structural representation of the present utility model.
Fig. 2 is the detection card body structure chart for placing the utility model test strips.
Embodiment
As shown in figure 1, the quick detection of detection Organophosphorus and carbamate pesticides pesticide residue described in the utility model
Test strips, including supporting back board 1 and the detection film being attached in supporting back board, detection film of the present utility model include which is provided with
The nitrocellulose filter 3 of detection band 2（Nitrocellulose filter 3 is NC films, 10 ~ 20mm of its length, wide 3 ~ 5mm）, detection band 2 is to draw
Fast red or solid blue reagent line on nitrocellulose filter；It is pasted with and nitre in the supporting back board in the left side of nitrocellulose filter 3
The absorbing membrane 4 that acid cellulose film is mutually overlapped, overlap joint is provided with substrate pad successively in the supporting back board on the right side of nitrocellulose filter
5th, enzyme piece 6 and sample pad 7.
Supporting back board 1 is using thickness 0.3 ~ 0.7mm polyvinyl chloride plastic sheets, and its length is 60 ~ 100mm, and width 3 ~
5mm。
Sample pad 7 uses 0.4 ~ 0.7mm of thickness, long 10 ~ 20mm, wide 3 ~ 5mm blotting paper；
Enzyme piece 6 is 0.2 ~ 0.5mm of thickness immersed with acetylcholinesterase, long 10 ~ 20mm, wide 3 ~ 5mm water adsorption glass fibre
Tie up film；During preparation, glass fibre membrane can be cut into the strip of above-mentioned size, active 2000U/ml acetylcholinesterase is immersed in
30min in solution, takes out after taking out 20min in vacuum freeze drier, dries；
Substrate pad 5 is 0.3 ~ 0.6mm of thickness immersed with ethychlozate ester, long 10 ~ 20mm, wide 3 ~ 5mm water suction polyester fibre
Film DL42 is tieed up, during preparation, polyester fiber film is cut into strip, 30min in 100mM heteroauxin ester solution is immersed in, is taken out
After taking out 5min in vacuum freeze drier, dry；
0.3 ~ 0.5mm of thickness of absorbing membrane 4, long 10 ~ 20mm, wide 3 ~ 5mm.
The lap of splice between adjacent two diaphragm is 1 ~ 2mm.
Shaping test strips can be placed in the detection card body 8 of flat shell shape（Supporting back board is pasted onto card body bottom）, such as
Shown in Fig. 2, detection fenestra 9 and well 10 are offered in the upper surface of detection card body 8, the test strips being placed in detection card body
Sample pad 7 is just to well 10, and the detection band 2 on nitrocellulose filter 3 is just to detection fenestra 9.
Reaction principle of the present utility model is：According to the diazol of respective concentration fixed on nitrocellulose filter（Fast red
Or solid blue reagent）Coupling reaction is occurred by the aromatic compound that substrate hydrolysis discharges processing with acetylcholinesterase, forms even
Nitrogen compound.If without agricultural chemicals, substrate ethychlozate ester by acetylcholine ester enzyme hydrolysis, discharge nucleophilic aromatic compounds of group with again
Nitrogen salt fast red or the solid blue azo-compound for occurring coupling reaction, generation brownish red or blueness, with appearing in chromatographic medium surface
Function group formation ion/covalent bond, therefore index strip can be formed on film；If there is agricultural chemicals, acetylcholinesterase is by agriculture
Medicine suppresses, and substrate hydrolysis is restricted, it is impossible to carry out the coupling reaction of next step, and then shows that at index strip discoloration is weak or nothing
Color.The color finally shown by it indicates the presence or absence of acetylcholinesterase, so as to judge organophosphorus insecticide and amino first
The content of esters of gallic acid agricultural chemicals.
Method using the residual content of ELISA test strip fruits and vegetables middle peasant of the present utility model is as follows：
1st, vegetable sample to be measured is cut into 1cm or so square fragments, takes 1g to be put into small beaker, add 5mL bufferings molten
Liquid, vibrates 1min ~ 2min, pours out sample extracting solution, stands 3min ~ 5min stand-by；
Cushioning liquid wherein used is：11.9g ADKPs and 3.2g potassium dihydrogen phosphates are taken respectively, are distilled with 1L
Water dissolving was both obtained；
2nd, sample liquid to be detected is added dropwise to from well 10 in sample pad 7, due to siphonage principle, sample liquid
Substrate pad 5 is reached by enzyme piece 6, then to diffusion on nitrocellulose filter 3, with the fast red being fixed on nitrocellulose filter 3
Or solid blue reagent（Detection band 2）Generation coupling reaction.
3rd, under the conditions of 25 DEG C, the color change of detection band 2 in detection fenestra 9, key reaction are visually observed in 5 ~ 15 minutes
As a result there are following two situations：
A, when detection band 2 shows brownish red or blue marking band, it is feminine gender to represent testing result, illustrate detected sample
Residues of pesticides are not exceeded；
B, when the not display color or faint yellow of detection band 2, it is the positive to represent testing result, illustrate that detected sample agricultural chemicals is residual
Stay exceeded.
Claims (4)
1. a kind of Rapid detection test strip for detecting Organophosphorus and carbamate pesticides pesticide residue, including supporting back board（1）With
It is attached to the detection film in the supporting back board, it is characterised in that：The detection film includes which is provided with detection band（2）Nitric acid
Cellulose membrane（3）, in the nitrocellulose filter（3）It is pasted with and is mutually taken with the nitrocellulose filter in the supporting back board in left side
The absorbing membrane connect（4）, overlap joint is provided with substrate pad successively in the supporting back board on the right side of the nitrocellulose filter（5）, enzyme piece
（6）And sample pad（7）.
2. the Rapid detection test strip of detection Organophosphorus and carbamate pesticides pesticide residue according to claim 1, its
It is characterised by：The supporting back board（1）The polyvinyl chloride plastic sheets for being 0.3 ~ 0.7mm for thickness, its length is 60 ~ 100mm,
3 ~ 5mm of width.
3. the Rapid detection test strip of detection Organophosphorus and carbamate pesticides pesticide residue according to claim 1, its
It is characterised by：The sample pad（7）For 0.4 ~ 0.7mm of thickness, long 10 ~ 20mm, wide 3 ~ 5mm blotting paper；The enzyme piece（6）For leaching
Bubble has 0.2 ~ 0.5mm of thickness of acetylcholinesterase, long 10 ~ 20mm, wide 3 ~ 5mm water adsorption glass tunica fibrosa；The substrate pad（5）
For 0.3 ~ 0.6mm of thickness immersed with ethychlozate ester, long 10 ~ 20mm, wide 3 ~ 5mm water imbibition polyester fiber film；The absorbing membrane
（4）0.3 ~ 0.5mm of thickness, long 10 ~ 20mm, wide 3 ~ 5mm.
4. the Rapid detection test strip of detection Organophosphorus and carbamate pesticides pesticide residue according to claim 1, its
It is characterised by：The nitrocellulose filter（3）For NC films, 10 ~ 20mm of its length, wide 3 ~ 5mm, the detection band（2）To draw
Fast red or solid blue reagent line on the nitrocellulose filter.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621203784.9U CN206399840U (en) | 2016-11-08 | 2016-11-08 | Detect the Rapid detection test strip of Organophosphorus and carbamate pesticides pesticide residue |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621203784.9U CN206399840U (en) | 2016-11-08 | 2016-11-08 | Detect the Rapid detection test strip of Organophosphorus and carbamate pesticides pesticide residue |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206399840U true CN206399840U (en) | 2017-08-11 |
Family
ID=59507006
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201621203784.9U Active CN206399840U (en) | 2016-11-08 | 2016-11-08 | Detect the Rapid detection test strip of Organophosphorus and carbamate pesticides pesticide residue |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206399840U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109884041A (en) * | 2019-02-14 | 2019-06-14 | 广州奕昕生物科技有限公司 | A kind of Fast Determination of Pesticide Residue card |
| CN114113247A (en) * | 2020-08-31 | 2022-03-01 | 浙江纳智汇生物科技有限公司 | Electrochemical detection method, detection chip, detection kit and detection system based on enzyme inhibition method |
-
2016
- 2016-11-08 CN CN201621203784.9U patent/CN206399840U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109884041A (en) * | 2019-02-14 | 2019-06-14 | 广州奕昕生物科技有限公司 | A kind of Fast Determination of Pesticide Residue card |
| CN109884041B (en) * | 2019-02-14 | 2021-04-06 | 广州奕昕生物科技有限公司 | Pesticide residue short-term test card |
| CN114113247A (en) * | 2020-08-31 | 2022-03-01 | 浙江纳智汇生物科技有限公司 | Electrochemical detection method, detection chip, detection kit and detection system based on enzyme inhibition method |
| CN114113247B (en) * | 2020-08-31 | 2024-05-14 | 浙江纳智汇生物科技有限公司 | Electrochemical detection method, detection chip, detection kit and detection system based on enzyme inhibition method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Khan et al. | Monitoring health implications of pesticide exposure in factory workers in Pakistan | |
| CN102816691B (en) | Device for quickly detecting pesticide residues in fruits and vegetables, and application thereof | |
| CN206399840U (en) | Detect the Rapid detection test strip of Organophosphorus and carbamate pesticides pesticide residue | |
| CN101299029A (en) | Preparation for detecting organophosphorus pesticide residual test paper and its use method | |
| CN102692388A (en) | Digital imaging system and method for rapid detection of organophosphorus pesticide residues in fruits and vegetables by using same | |
| CN106501250A (en) | A kind of Rapid detection test strip and the method using the ELISA test strip Organophosphorus and carbamate pesticides pesticide residue | |
| CN203502356U (en) | Test strip for quickly detecting pesticide residues in fruits and vegetables | |
| CN105928929A (en) | Rapid detection point card for pesticide residues and method for detecting content of pesticide residues by using detection point card | |
| CN103421880A (en) | Detection method of organophosphorus pesticide | |
| CN101598716B (en) | Test strip for fast testing gossypol and preparation method and application thereof | |
| WO2004099754A2 (en) | Sensor for detecting compounds | |
| Han et al. | Chromogenic platform based on recombinant Drosophila melanogaster acetylcholinesterase for visible unidirectional assay of organophosphate and carbamate insecticide residues | |
| Tavallali et al. | A highly selective optode for determination of Hg (II) by a modified immobilization of indigo carmine on a triacetylcellulose membrane | |
| CN205719999U (en) | Formaldehyde in food Test paper | |
| CN103712978A (en) | Simple portable pesticide residue detector | |
| CN105675606A (en) | Quick detection card for pesticide residues | |
| CN105928940A (en) | Pesticide residue detection method and detection kit | |
| Xu et al. | Comparison of four commercial enzymatic assay kits for the analysis of organophosphate and carbamate insecticides in vegetables | |
| CN104297179A (en) | Reagent used for detecting N-acetyl-beta-D-glucosaminidase | |
| CN1271400C (en) | Rapid determination method for organophosphorus pesticide residue in vegetable and fruit | |
| CN106198510A (en) | Fast Determination of Pesticide Residue card and pesticide residue detection method | |
| CN206594063U (en) | A kind of broad-spectrum pesticide remains quick detection plate | |
| CN104076036B (en) | The method of biguanides in detection goods | |
| CN204832045U (en) | Organic phosphorus pesticide quick test card | |
| CN102507880A (en) | Card for quickly detecting acrylamide, and detection method therefor |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |