CN205874450U - Light intensity light kind of controllable plant cell or little algae culture apparatus - Google Patents

Light intensity light kind of controllable plant cell or little algae culture apparatus Download PDF

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Publication number
CN205874450U
CN205874450U CN201620774639.XU CN201620774639U CN205874450U CN 205874450 U CN205874450 U CN 205874450U CN 201620774639 U CN201620774639 U CN 201620774639U CN 205874450 U CN205874450 U CN 205874450U
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CN
China
Prior art keywords
light
plant cell
ventilative
euphotic cover
illumination
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Expired - Fee Related
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CN201620774639.XU
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Chinese (zh)
Inventor
张毅宁
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Zhejiang Province Hangzhou Xuejun Middle School
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Zhejiang Province Hangzhou Xuejun Middle School
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Abstract

The utility model discloses a light intensity light kind of controllable plant cell or little algae culture apparatus, include: a stainless steel blake bottle for training plant cell or little algae, with stainless steel blake bottle screw -thread fit's ventilative euphotic cover, ventilative euphotic cover include that gas -permeable section divides and light transmissive portion, the setting is in electron illumination regulation and control device on the light transmissive portion of ventilative euphotic cover. The utility model discloses the device is applicable to the condition control that carries out plant cell or little algae illumination intensity, light kind of colour when training research in the laboratory, and culture apparatus wholly can be fixed in the shaking table or place in the incubator, than the traditional light shine train the frame or incubator strength of material big, low cost, easy operation, area is little, light conditions control is accurate, independence and avoid the dark environmental disturbance of external light, is applicable to the special research in laboratory.

Description

Plant cell that a kind of smooth high light kind is controlled or micro algae culturing device
Technical field
This utility model relates to plant cell or micro algae culturing device field, is specifically related to a kind of smooth controlled planting of high light kind Thing cell or micro algae culturing device.
Background technology
Plant cell or microdisk electrode are important technology in modern biology, for research novel plant or microalgae product Plant institute required.For the condition of culture of a kind of plant or microalgae cell, illumination factor, including light intensity and light kind, the most directly Connect relevant with every Basic Life activity of plant or microalgae, also can remote-effects to plant or microalgae cell output matter with Amount.In the research of plant or microalgae physiology, illumination is also a kind of important experimental variable that must consider.
The method of plant or the control illumination of microalgae cell culture technique generally uses illumination cultivation frame or illumination cultivation at present Condition broadly unified by case, or broadly calculates its illumination condition according to bright light quantity, and such method exists problem below:
1. accurate not because distribution of light sources is uneven, calculate institute's value and can not react real experiment condition very well;
2. easily affected by laboratory daylight lamp opening and closing outside external environment such as culturing room;
3. single culture bottle can not be carried out concrete regulation and control and analysis;
4. need when processing a small amount of sample to spend substantial amounts of energy and ample resources to meet a certain specified conditions, if desired for many Individual illumination box;
5. can not eliminate interacting of the different experimental conditions in same culturing room, processing different experiments when The biggest demand is had for culture space;
In sum, traditional illumination cultivation frame or illumination box illumination when plant or microalgae cell are cultivated become Amount control method exist easily be disturbed, error is big, required experimental period and the limitation such as space is big, equipment is many.
Application publication number is that the Chinese invention patent application of CN 101724547A (Application No. 200910258258.0) is public Having opened a kind of LED tunable optical and carried out the device of microalgae culture experiment, this device is controlled by casing, illumination control system and temperature System forms, and in the casing of device, every tabula rasa, whole box house is divided into upper and lower two parts, on box house with ventilation Half both sides, equipment LED luminescence component and LED control circuit, at both sides, box house lower half equipment daylight lamp luminescence component and Daylight lamp control circuit.This technical scheme can select the strong Non-follow control of continuous light respectively and automatically control, optional spectrum and light According to the cycle, it is also possible to select the whole day of simulation daylight gradual change to automatically control illumination pattern, but this device is complicated and the most stupid Weight, it is the most convenient to use.
Utility model content
This utility model provides the controlled plant cell of a kind of smooth high light kind or micro algae culturing device, and the strength of materials is big, With low cost, simple to operate, floor space is little, and condition controls accurately, it is adaptable to the special item of laboratory.
Plant cell that a kind of smooth high light kind is controlled or micro algae culturing device, including:
For cultivating the rustless steel culture bottle of plant cell or microalgae;
With the ventilative euphotic cover of described rustless steel culture bottle threaded engagement, described ventilative euphotic cover include permeable portion and Light transmission part;
The electronic light regulating and controlling illumination device being arranged on the light transmission part of described ventilative euphotic cover.
Following as optimal technical scheme of the present utility model:
The body of described rustless steel culture bottle uses stainless steel material to make, intensity height, lucifuge, heat conduction soon, easy cleaning, can High pressure steam sterilization or dry heat sterilization.
The bottle mouth position of described rustless steel culture bottle is provided with the external screw thread coordinated with described ventilative euphotic cover, and described is ventilative The female thread matched with described external screw thread it is provided with in euphotic cover.
Ventilative euphotic cover, is made with glass material by rustless steel, under oriented female thread, rustless steel make, with culture bottle Body matches.
Described ventilative euphotic cover outer wall is provided with coarse lines, facilitates user both to be tightened.
Described ventilative euphotic cover, caping is circular concentric, and in central authorities, region, the center of circle is permeable portion, is provided with multiple circle little Hole, there is upwardly extending cylindrical permeability cell on border, has three vertical draw-in grooves, with electronic light regulating and controlling illumination device phase outside permeability cell Supporting, above border circular areas and permeability cell thereof are made by stainless steel material;Concentric circular outer region is made up of glass material, for Light transmission part, light transmission is fabulous, and size matches with electronics illumination apparatus.
The middle part of described ventilative euphotic cover is permeable portion, and this permeable portion is for cylindrical permeability cell and is arranged on described Air permeable plate in cylindrical permeability cell, described air permeable plate is provided with passage, and the excircle of described ventilative euphotic cover is Light part, can use the transparent material such as plastics, glass.
The outer hull shape such as hollow cylinder of described electronic light regulating and controlling illumination device, breathing freely of the most ventilative euphotic cover of height Pipe, bottom surface annulus size is identical with the glass outer region size of ventilative euphotic cover.The electronic light regulating and controlling illumination of hollow cylinder build Crust of the device is made up of plastic material, is divided into upper and lower two parts, between by screw thread bayonet connection, the electricity of whole hollow cylinder build Sub-illumination regulation device medial surface has the card article protruded inwardly from, with the outer side bayonet slot of permeability cell that described ventilative printing opacity cultivates lid arrangement Match.
Described electronic light regulating and controlling illumination device is hollow cylinder, is set on described cylindrical permeability cell.
The outer wall of described cylindrical permeability cell is provided with draw-in groove, and described electronic light regulating and controlling illumination device is provided with and described draw-in groove The card article coordinated.
Described electronic light regulating and controlling illumination device includes that annulus base and annulus upper cover, described annulus base are provided with illumination Lamp, described annulus is covered with key switch.
Described annulus base and annulus upper cover use threaded, and described annulus base is mounted with operating circuit and electricity Pond, also illumination lamp (can specifically use cold light lamp), threaded, is easy to open dismounting, replaces battery or maintenance.
Further preferably, described illumination lamp is 8, and described key switch is 4, and 1 key switch controls 2 light Illuminator, can control different illumination lamp and light intensity by different key switches, and as by 1 key switch, 2 illumination lamps are the brightest , simultaneously by 4 key switches, then 8 all light of illumination lamp.
Electronic light regulating and controlling illumination crust of the device shape such as hollow cylinder, the permeability cell of height the most ventilative printing opacity cultivation lid, Bottom surface annulus size is identical with the glass outer region size of ventilative euphotic cover.The electronic light regulating and controlling illumination device of hollow cylinder build Shell is made up of plastic material, is divided into upper and lower two parts: top is divided into vinyl cover, and the oriented female thread of its lower end inner wall, with bottom Distribution set connects, and four switches are arranged at top, and luminous environment when can control its intensity of illumination and react its work is identified with device light Kind and model identification (be such as designated Purple:0~4000Lux/1000Lux, i.e. represent this device can provide 0~ 4000Lux, 1000Lux are the purple light of a grade);Lower part loading operating circuit and battery, also light source (such as cold light source lamp), its Upper end inwall is provided with outside screw thread, supporting with upper part is connected.In the electronic light regulating and controlling illumination device of whole hollow cylinder build There is the card article protruded inwardly from side, and outer side bayonet slot matches with the permeability cell of described ventilative euphotic cover.
Ventilative cylindrical permeability cell height designed by euphotic cover is higher than the electronic light regulating and controlling illumination device of hollow cylinder build, Top can install ventilative Nostoc commune Vanch bottle closure film or tampon additional, it is possible to use rubber closure is airtight, it is provided that different cells are cultivated The anaerobic of Shi Suoxu and aerobic environment.
The light source of the different light intensity of electronic light regulating and controlling illumination device application and light kind is combined, energy-conserving and environment-protective, and will not be to training Support bottle internal temperature to impact;Electronic light regulating and controlling illumination installation's power source application button cell, it is simple to change, make this device lifetime more Long;Electronic light regulating and controlling illumination device is provided with individually switch, it is simple to meets experiment sample and sets the regulated variable of illumination condition Control.
Contrast prior art, improvement effect of the present utility model is:
1. this utility model light high light kind is controlled plant cell or micro algae culturing device entirety major applications rustless steel Making, fraction application glass and plastics are made, with low cost, more sturdy and durable, and good heat conductivity overcomes Nostoc commune Vanch The shortcoming that bottle is frangible, is also easier to reach temperature conditions required when cultivating, and meanwhile, lighttight design is completely eliminated in reality During testing, culture bottle inner cell illumination condition is caused by the external condition such as the transfer of experiment material and external environment condition brightness change Interference and impact;
2. cultivate body inner wall smooth, it is simple to clean, it is possible to application ultrasonic washing, whole in addition to electronic light regulating and controlling illumination device Body can autoclaving, it is ensured that aseptic condition in culture bottle;The outside available alcohol wipe surface sterilization of electronic light regulating and controlling illumination device, easily In creating clean experimental situation;
3. cultivate between body and light transmission ventilation lid and electronic light regulating and controlling illumination device detachable, and without changing the behaviour such as bottle during dismounting Make, apply different types of electronic light regulating and controlling illumination device, the different rings of light cultivated needed for different phase can be built the most easily Border;
4. electronic light regulating and controlling illumination device can specifically use cold light source, energy-conserving and environment-protective, and will not make culture bottle internal temperature Become impact;
5. electronic light regulating and controlling illumination installation's power source application button cell, it is simple to change, make this device lifetime longer;
6. electronic light regulating and controlling illumination device is provided with individually switch, it is simple to meets experiment sample and becomes the regulation of illumination condition Amount setup control;
7. the permeability cell top designed by euphotic cover of breathing freely can install ventilative Nostoc commune Vanch bottle closure film or tampon additional, also Rubber closure can be used airtight, it is provided that anaerobic required during different cells cultivation and aerobic environment;
8. culture apparatus entirety is securable on shaking table, it is simple to ventilation during cultivation and mixing, it is to avoid sample pellet, simultaneously The culture bottle of different illumination conditions can be positioned on same shaking table, had both ensured the temperature between laboratory sample different disposal group, concussion The concordance of the condition of culture such as rotating speed, can ensure its independent effectiveness by the illumination condition accurately controlled in each culture bottle again, exempt from By external interference and avoid influencing each other between culture bottle, save culture space and resource, improve experimentation efficiency And accuracy.
9, intensity of illumination, light when plant cell or microdisk electrode research is carried out in this utility model device is applicable to laboratory The condition planting color controls, and culture apparatus entirety is securable to shaking table or is positioned over incubator, than traditional illumination cultivation frame or training Supporting box material intensity big, with low cost, simple to operate, floor space is little, and illumination condition controls accurate, independence and avoids ambient light Dark situation disturbs, it is adaptable to the special item of laboratory.
Accompanying drawing explanation
Fig. 1 is the controlled plant cell of this utility model light high light kind or the structural representation of micro algae culturing device;
Fig. 2 is annulus base and the structural representation of annulus upper cover of electronic light regulating and controlling illumination device in this utility model;Its In, in Fig. 2, (a) is the structural representation of annulus base, and in Fig. 2, (b) is the structural representation of annulus upper cover;
Fig. 3 is the structural representation of ventilative euphotic cover in this utility model.
Detailed description of the invention
Below in conjunction with the accompanying drawings the controlled plant cell of this utility model light high light kind or micro algae culturing device are made further Describe in detail.
In conjunction with the drawings and specific embodiments, the utility model is described in further detail.Should be understood that these embodiments are only used for This utility model is described rather than limits scope of the present utility model.In addition, it is to be understood that reading this utility model instruction Content after, this utility model can be made various changes or modifications by those skilled in the art, and these equivalent form of values fall equally Enter the application appended claims limited range.
As shown in Figure 1, Figure 2, Figure 3 shows, plant cell that a kind of smooth high light kind is controlled or micro algae culturing device, including: it is used for Cultivate plant cell or the rustless steel culture bottle 2 of microalgae;With the ventilative euphotic cover of rustless steel culture bottle 2 threaded engagement, breathe freely thoroughly Light lid includes permeable portion and light transmission part;The electronic light regulating and controlling illumination device being arranged on the light transmission part of ventilative euphotic cover.No The body of rust steel culture bottle 2 uses stainless steel material to make, intensity height, lucifuge, heat conduction soon, easy cleaning, can high pressure steam sterilization Or dry heat sterilization.
The bottle mouth position of rustless steel culture bottle 2 is provided with the external screw thread 3 coordinated with euphotic cover of breathing freely, and ventilative euphotic cover is provided with outward The female thread 4 that screw thread 3 matches.
The middle part of ventilative euphotic cover is permeable portion, and this permeable portion is for cylindrical permeability cell 6 and is arranged on cylinder thoroughly Air permeable plate 10 in trachea 6, air permeable plate 10 is provided with passage, and (euphotic cover top of i.e. breathing freely is same for the excircle of ventilative euphotic cover Heart circle outer ring portion 8) it is light transmission part.
Ventilative euphotic cover, caping is circular concentric, and in central authorities, region, the center of circle is permeable portion, is provided with multiple circular aperture (i.e. passage), there is upwardly extending cylindrical permeability cell 6 on border, has three vertical draw-in grooves 7 outside cylindrical permeability cell 6, with Electronic light regulating and controlling illumination device matches, and above border circular areas and permeability cell thereof are made by stainless steel material;Concentric circular outer portion Dividing 8 to be made up of glass material, for light transmission part, light transmission is fabulous, and size matches with electronics illumination apparatus.
Electronic light regulating and controlling illumination device is hollow cylinder, is set on cylindrical permeability cell 6.
The outer wall of cylindrical permeability cell 6 is provided with draw-in groove 7, and electronic light regulating and controlling illumination device is provided with the card article 9 coordinated with draw-in groove 7.
Electronic light regulating and controlling illumination device includes annulus base and annulus upper cover, and annulus base is provided with illumination lamp 11, illumination lamp 11 can use cold light lamp, and annulus is covered with key switch 1.
Annulus base and annulus upper cover use threaded, and annulus base is mounted with operating circuit and battery, also illumination Lamp 11 (can specifically use cold light lamp), threaded, it is easy to open dismounting, replaces battery or maintenance.
Illumination lamp is 8, and key switch is 4, and 1 key switch controls 2 illumination lamps, can open by different buttons Closing, control different illumination lamp and light intensity, as by 1 key switch, 2 illumination lamps are the brightest, simultaneously by 4 key switches, then 8 all light of illumination lamp.
Plant cell or microalgae are mixed with culture fluid and is placed in rustless steel culture bottle 2 cultivation, on rustless steel culture bottle 2 Portion's bottleneck is furnished with external screw thread 3, cultivates matching to female thread 4 of lid bottom with ventilative printing opacity, makes ventilative euphotic cover be fixed on not With rust steel culture bottle 2, play the effect of sealing, have coarse lines 5 outside ventilative euphotic cover, facilitate user both to be twisted Tightly, ventilative euphotic cover has cylindrical permeability cell 6, cylindrical permeability cell 6 height slightly above electronic light regulating and controlling illumination device, Ke Yiwei The covering leaving space of sealed membrane, maintains the gnotobasis within rustless steel culture bottle 2, it is also possible to fill in rubber closure, builds nothing Collarium border, cylindrical permeability cell 6 is outside draw-in groove 7, matches with the card article 9 on electronic light regulating and controlling illumination device, for fixing electricity Sub-illumination regulation device, has air permeable plate 10 at cylindrical permeability cell 6 inner bottom part, it is simple to the air friendship that culture bottle is internal with outside Change.The above is rustless steel and makes.Ventilative euphotic cover top concentric circular outer ring portion 8 is made up of clear glass, with electronics Illumination lamp 11 (specifically using cold light lamp) bottom illumination regulation device is corresponding, it is simple to electronic light regulating and controlling illumination device is launched Light is fully penetrating in rustless steel culture bottle 2, is also prevented from the entrance of extraneous light;Electronic light regulating and controlling illumination device application button cell Power supply, can change battery, need to will outward winding with threaded sealing 12 during replacing, and battery case is i.e. arranged at bottom, can be by waste and old electricity New battery is taken out and loads in pond, maintains device normal working performance, and the key switch 1 at electronic light regulating and controlling illumination device top has Four, each key switch 1 controls two illumination lamps 11 (specifically using cold light lamp) of bottom symmetrical, and key switch 1 is beaten Little red light internal after holding can light, and represents that this switch is opened, it is simple to user is accurate in the case of not provision for disengagement Intensity of illumination in ground regulation bottle.
Embodiment 1:
Such as apply the controlled plant cell of this light high light kind or micro algae culturing device research light intensity for pyrenoids bead The impact of algae (microalgae) growth rate.I.e. use specification be white light: 0~4000Lux/1000Lux (can provide 0~ 4000Lux, 1000Lux are the white light of a grade) device, experimental group can arrange be not switched on switch (0Lux), open a button Switch 1 (1000Lux), opens two key switches 1 (2000Lux), opens three key switches 1 (3000Lux), open four The illumination condition of key switch 1 (4000Lux).The Chlorella pyrenoidesa suspension of inoculation equivalent, shaking in being placed in control environment After cultivating a period of time on bed, take part culture fluid and use blood cell counting plate can calculate Chlorella pyrenoidesa (microalgae) in it Quantity, i.e. can reach experiment purpose.
Embodiment 2:
Such as apply the controlled plant cell of this light high light kind or micro algae culturing device research light kind for Flos Chrysanthemi wound healing group Knit the impact of (plant cell) growth rate.I.e. using specification is white light (White): 0~4000Lux/1000Lux (can to carry For 0~4000Lux, 1000Lux is the white light of a grade), HONGGUANG (Red): 0~4000Lux/1000Lux (can provide 0~ 4000Lux, 1000Lux are the HONGGUANG of a grade), blue light (Blue): 0~4000Lux/1000Lux (can provide 0~ 4000Lux, 1000Lux are the blue light of a grade), green glow (Green): 0~4000Lux/1000Lux (can provide 0~ 4000Lux, 1000Lux are the green glow of a grade) device of four kinds, experimental group can all be opened two key switches 1 and (provide The illumination of 2000Lux), it is respectively provided with the luminous environment of white light, HONGGUANG, blue and green light, adds equivalent chrysanthemum callus suspension, After cultivating a period of time on shaking table in being placed in control environment, take part culture fluid and use blood cell counting plate can calculate it Interior chrysanthemum callus cell quantity, i.e. can reach experiment purpose.
Embodiment 3:
Such as apply the controlled plant cell of this light high light kind or micro algae culturing device research photoperiod for Radix Dauci Sativae wound healing The impact of histiocyte (plant cell) growth rate.I.e. using specification is white light: 0~4000Lux/1000Lux (can provide 0~4000Lux, 1000Lux are the white light of a grade) device, experimental group can all be opened two key switches 1 and (provide 2000Lux Illumination), the closedown light source of certain time is set, illumination in 14 hours 10 hours is dark, and illumination in 12 hours 12 hours is dark, and 10 is little 14 hours dark of Shi Guangzhao, 16 hours dark illumination conditions of illumination in 8 hours.Inoculation equivalent carrot callus cell is cultivated Liquid, after a period of time cultivated by the shaking table in being placed in control environment, takes part culture fluid and uses blood cell counting plate to count Calculate carrot callus's cell (plant cell) quantity in it, i.e. can reach experiment purpose.
The technical solution of the utility model and beneficial effect have been described in detail by above-described detailed description of the invention, It it should be understood that and the foregoing is only most preferred embodiment of the present utility model, be not limited to this utility model, all at this Any amendment of being made in the spirit of utility model, supplement and equivalent etc., should be included in guarantor of the present utility model Within the scope of protecting.

Claims (8)

1. a light high light kind is controlled plant cell or micro algae culturing device, it is characterised in that including:
For cultivating the rustless steel culture bottle of plant cell or microalgae;
With the ventilative euphotic cover of described rustless steel culture bottle threaded engagement, described ventilative euphotic cover includes permeable portion and printing opacity Part;
The electronic light regulating and controlling illumination device being arranged on the light transmission part of described ventilative euphotic cover.
Plant cell that smooth high light kind the most according to claim 1 is controlled or micro algae culturing device, it is characterised in that described The bottle mouth position of rustless steel culture bottle be provided with the external screw thread coordinated with described ventilative euphotic cover, be provided with in described ventilative euphotic cover The female thread matched with described external screw thread.
Plant cell that smooth high light kind the most according to claim 1 is controlled or micro algae culturing device, it is characterised in that described The middle part of ventilative euphotic cover be permeable portion, this permeable portion is for cylindrical permeability cell and is arranged on described cylindrical permeability cell Interior air permeable plate, described air permeable plate is provided with passage, and the excircle of described ventilative euphotic cover is light transmission part.
Plant cell that smooth high light kind the most according to claim 3 is controlled or micro algae culturing device, it is characterised in that described Electronic light regulating and controlling illumination device be hollow cylinder, be set on described cylindrical permeability cell.
Plant cell that smooth high light kind the most according to claim 4 is controlled or micro algae culturing device, it is characterised in that described The outer wall of cylindrical permeability cell be provided with draw-in groove, described electronic light regulating and controlling illumination device is provided with and the card article of described card slot fit.
Plant cell that smooth high light kind the most according to claim 1 is controlled or micro algae culturing device, it is characterised in that described Electronic light regulating and controlling illumination device include that annulus base and annulus upper cover, described annulus base are provided with illumination lamp, described circle Key switch it is covered with on ring.
Plant cell that smooth high light kind the most according to claim 6 is controlled or micro algae culturing device, it is characterised in that described Annulus base and annulus upper cover use threaded.
Plant cell that smooth high light kind the most according to claim 6 is controlled or micro algae culturing device, it is characterised in that described Illumination lamp be 8, described key switch is 4, and 1 key switch controls 2 illumination lamps.
CN201620774639.XU 2016-07-19 2016-07-19 Light intensity light kind of controllable plant cell or little algae culture apparatus Expired - Fee Related CN205874450U (en)

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Application Number Priority Date Filing Date Title
CN201620774639.XU CN205874450U (en) 2016-07-19 2016-07-19 Light intensity light kind of controllable plant cell or little algae culture apparatus

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Application Number Priority Date Filing Date Title
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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107853180A (en) * 2017-12-05 2018-03-30 福建农林大学 The tissue culture device and its cultural method of integral type controllable light
CN108485936A (en) * 2018-05-15 2018-09-04 中国科学技术大学 A kind of microalgae experimental provision
CN109355182A (en) * 2018-12-14 2019-02-19 上海光音照明技术有限公司 A kind of LED based illumination box

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107853180A (en) * 2017-12-05 2018-03-30 福建农林大学 The tissue culture device and its cultural method of integral type controllable light
CN108485936A (en) * 2018-05-15 2018-09-04 中国科学技术大学 A kind of microalgae experimental provision
CN108485936B (en) * 2018-05-15 2024-03-29 中国科学技术大学 Microalgae experimental device
CN109355182A (en) * 2018-12-14 2019-02-19 上海光音照明技术有限公司 A kind of LED based illumination box

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Granted publication date: 20170111

Termination date: 20170719