CN205794638U - A kind of spiral cover bushing type rareness sperm superthin section freezing carrier - Google Patents
A kind of spiral cover bushing type rareness sperm superthin section freezing carrier Download PDFInfo
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- CN205794638U CN205794638U CN201620669425.6U CN201620669425U CN205794638U CN 205794638 U CN205794638 U CN 205794638U CN 201620669425 U CN201620669425 U CN 201620669425U CN 205794638 U CN205794638 U CN 205794638U
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Abstract
This utility model relates to a kind of spiral cover bushing type rareness sperm superthin section freezing carrier, and described spiral cover bushing type rareness sperm superthin section freezing carrier includes trocar sheath, freezing slide glass, knob lid；Described freezing slide glass one end is fixed on knob lid, and the other end inserts in trocar sheath；Described trocar sheath end connects knob lid；Described trocar sheath one end is provided with external screw thread；Described knob lid includes sub-knob lid and female knob lid；The medial surface of described sub-knob lid and female knob lid is equipped with female thread；It is provided with sub-snap close outside described sub-knob lid；It is provided with locking device and a female outside described female knob；It is additionally provided with platform on described sub-knob lid, and platform is provided with fixed groove；One end of described slide glass is additionally provided with circular indicia；The other end of described slide glass is additionally provided with fixing strip, handle；Described fixing strip matches with fixed groove.Its advantage shows: frozen effective, easy to operate, simple in construction, and the sperm response rate is high, and after recovery, living spermatozoa percentage is high.
Description
Technical field
This utility model relates to animal reproduction and human reproduction field spermatozoa cryopreservation technical field, specifically, is a kind of
Spiral cover bushing type rareness sperm superthin section freezing carrier.
Background technology
Auxiliary procreation technology (Assisted Reproductive Technology is called for short ART) is to oligospermia or nothing
Essence disease patient (obtaining the sperm that quantity is few by testis, epididymis biopsy) carries out Intracytoplasmic Sperm Injection treatment
(Intracytoplasmic sperm injection, be called for short ICSI) and obtain filial generation.The gestation of each ICSI treatment cycle
Rate is at 35%-45%, and in order to obtain gestation, some patients needs repeatedly ICSI treatment.If it is not the dilutest to it
Few sperm carries out frozen, then treatment will carry out testis, epididymis biopsy every time, and the sperm of oligospermia patient is unstable, also has
Sperm may be can not get so that cancelling treatment.
Freezing (2-3 bar/LPF) to rare sperm, the report of the current various freezing carrier of common use, Cohen and
Desai is respectively adopted zona pellucida as the frozen rare sperm of freezing carrier, Nawroth use freezing ring be carrier freezing testis,
The sperm that epididymis obtains；There is the most again the side of " without the method for vitrification freezing rareness sperms of cryoprotective agent " such as Lu Guang
Method is reported.But cut both ways although the studies above has succeeded.There is foreign protein in zona pellucida, it is understood that there may be biological dirty
The risk of dye；Freezing ring is expensive, and the sperm obtaining testis, epididymis may need several freezing ring to carry out freezing, becomes
This increase.Without the open freezing straw of cryoprotective agent, there is the problem that liquid nitrogen pollutes.Current cryopreservation sperm
Storage tube, freezing straw etc. to the sperm motility after the Cryopreservation of rare sperm, sperm morphology, Sperm acrasomal integvity and
The functional parameters such as DNA break index are not the most satisfactory.
Therefore, needing badly and want a kind of frozen effective, easy to operate, simple in construction, the sperm response rate is high, and after recovery, sperm is deposited
The spiral cover bushing type rareness sperm superthin section freezing carrier that motility rate is high.
Summary of the invention
The purpose of this utility model is to provide a kind of frozen effective, easy to operate, simple in construction, and the sperm response rate is high,
The spiral cover bushing type rareness sperm superthin section freezing carrier that after recovery, living spermatozoa percentage is high.
For achieving the above object, this utility model adopts the technical scheme that:
A kind of spiral cover bushing type rareness sperm superthin section freezing carrier, described spiral cover bushing type rareness sperm superthin section is freezing
Carrier includes trocar sheath, freezing slide glass, knob lid；Described freezing slide glass one end is fixed on knob lid, outside the other end inserts
In sleeve pipe；Described trocar sheath end connects knob lid；Described trocar sheath one end is provided with external screw thread；In the described trocar sheath other end
Portion is cone: described knob lid includes sub-knob lid and female knob lid；Described sub-knob lid and the inner side of female knob lid
Face is equipped with female thread, and female thread matches with the external screw thread on trocar sheath；Described sub-knob lid and female knob lid pass through
Fold mechanism connects；It is provided with sub-snap close outside described sub-knob lid；It is provided with locking device and a female outside described female knob；Described son
It is additionally provided with platform on knob lid, and platform is provided with fixed groove；One end of described slide glass is additionally provided with circular indicia；Described slide glass
The other end be additionally provided with fixing strip, handle；Described fixing strip matches with fixed groove.
Further, described trocar sheath two ends are hatch frame.
Further, circular indicia has two, and each circular indicia internal volume is 2 microlitres.
Further, a length of 23.5 millimeters of described slide glass, width is 8 millimeters.
Further, described trocar sheath internal diameter be 10mm slide glass width be 8mm.
Further, described trocar sheath is prepared by heatproof high molecule material.
Further, the height of described handle is 6.2 millimeters, and width is 4.95 millimeters.
Further, described handle is provided with triangle mark.
Further, described fixing strip is rectangle.
This utility model advantage is:
1, a kind of spiral cover bushing type rareness sperm superthin section freezing carrier of the present utility model, frozen effective, operation side
Just, simple in construction, the sperm response rate is high, and after recovery, living spermatozoa percentage is high；
2, being provided with freezing slide glass, and freezing slide glass one end sets circular indicia, wall is thin, transparent, it is simple to technical staff is being inverted
Can clearly observe during operated under microscope, quickly freezing few sperm can will be needed to put into circular indicia；
3, the circular indicia on freezing slide glass, volume is 2 microlitres, and device volume is little, and the sperm of recovery is easily found, and sperm returns
Yield is high；
4, it is provided with trocar sheath, the direct contact of liquid nitrogen in freezing slide glass is contained in the sleeve pipe of sealing, can be effectively prevented from,
Decrease the chance of cross-contamination/infection；
5, being provided with knob lid, its knob lid connects freezing slide glass by fixed groove is fixing, knob lid by female thread with
External screw thread on trocar sheath is fixing to be connected, and knob lid uses equivalent padlock mode, and easy to use, good stability, during use
It is not concerned about turning on one's side or reverse:
6, a kind of spiral cover bushing type rareness sperm superthin section freezing carrier of the present utility model, use micrurgy+
Microdroplet lid oil process carrys out freezing rareness (single) sperm, after selecting sperm end of operation under microscope, carrier is taken out culture dish
Remove the oil covered on carrier again, can put in the sleeve pipe of band spiral cover, put liquid nitrogen storage；
7, the sperm response rate is higher than 90%, recovery survival rate about 50%.It is frozen effective, easy to use, is suitable for clinic
Promote the use of.
Accompanying drawing explanation
Accompanying drawing 1 is a kind of spiral cover bushing type rareness sperm superthin section freezing carrier assembling structural representation of the present utility model
Figure.
Accompanying drawing 2 is knob lid and freezing slide glass attachment structure schematic diagram.
Accompanying drawing 3 is trocar sheath main apparent direction schematic diagram.
Accompanying drawing 4 is outer sleeve structure schematic diagram.
Accompanying drawing 5 is sub-knob lid main apparent direction schematic diagram.
Accompanying drawing 6 is knob lid structural representation.
The freezing slide glass main apparent direction schematic diagram of accompanying drawing 7.
Accompanying drawing 8 is freezing slide structure schematic diagram.
Detailed description of the invention
Below in conjunction with embodiment and referring to the drawings this utility model is further described.
The reference and the ingredient that relate in accompanying drawing are as follows:
1. trocar sheath 11. external screw thread
2. freezing slide glass 21. circular indicia
22. fixing strip 23. handles
24. trianglees identify 3. knob lids
31. female thread 32. fold mechanisms
33. sub-knob lid 331. platforms
The 332. sub-snap closes of fixed groove 333.
34. female knob lid 341. locking device and a female
Embodiment 1
Refer to Fig. 1 and Fig. 2, Fig. 1 is a kind of spiral cover bushing type rareness sperm superthin section freezing carrier of the present utility model
Assembling structural representation.Fig. 2 is knob lid 3 and freezing slide glass 2 attachment structure schematic diagram.A kind of spiral cover bushing type rareness sperm surpasses
Thin slice freezing carrier includes trocar sheath 1, freezing slide glass 2, knob lid；Described freezing slide glass 2 one end is fixed on knob lid 3,
The other end inserts in trocar sheath 1；Described trocar sheath 1 end connects knob lid 3.
Refer to Fig. 3 and Fig. 4, Fig. 3 is trocar sheath 1 main apparent direction schematic diagram.Fig. 4 is trocar sheath 1 structural representation.Described
Trocar sheath 1 two ends be hatch frame；Described trocar sheath 1 one end is provided with external screw thread 11, and the external screw thread 11 on trocar sheath 1 conveniently exists
The one hand-held sub-knob lid 33 equipped with freezing slide glass 2 twists in female knob lid 34 smoothly；In circle inside described trocar sheath 1 other end
Taper, its cone is easy to deposit in the liquid nitrogen container used by Sperm treatment: described trocar sheath internal diameter is that 10mm slide glass width is
8mm is in case slide glass is viscous with internal surface of sleeve pipe glutinous.
Refer to Fig. 5 and Fig. 6, Fig. 5 is sub-knob lid 33 main apparent direction schematic diagram.Fig. 6 is knob lid 3 structural representation.
Described knob lid 3 includes sub-knob lid 33 and female knob lid 34；Described sub-knob lid 33 and the medial surface of female knob lid 34
It is equipped with female thread 31, and female thread 31 matches with the external screw thread 11 on trocar sheath 1；Described sub-knob lid 33 and female knob
Lid 34 is connected by fold mechanism 32, and its fold mechanism 32 may select hinge or bearing pin；It is provided with outside described sub-knob lid 33
Sub-snap close 333；It is provided with locking device and a female 341 outside described female knob；Platform 331 it is additionally provided with on described sub-knob lid 33, and flat
Platform 331 is provided with fixed groove 332.
Refer to the main apparent direction schematic diagram of Fig. 7, Fig. 7 freezing slide glass 2.Fig. 8 is freezing slide glass 2 structural representation.Described cold
The one end freezing slide glass 2 is additionally provided with circular indicia 21, and circular indicia 21 has two, and each circular indicia internal volume is 2 microlitres；Institute
Stating freezing a length of 23.5 millimeters of slide glass 2, width is 8 millimeters；The other end of described freezing slide glass 2 be additionally provided with fixing strip 22,
Handle 23；Described fixing strip 22 is rectangle；Described fixing strip 22 matches with fixed groove 332；Described handle 23
Height be 6.2 millimeters, width is 4.95 millimeters；Described handle 23 is provided with triangle mark 24；Described freezing slide glass 2
Use high transparency to process through special process and there is stable tack.
It should be understood that the rare sperm described in Quan Wen includes that single sperm situation, trocar sheath 1 are heatproof high molecule
Prepared by material, it is ensured that the sperm of freezing slide glass 1 freezing is free of extraneous thermal effects, and refrigerating effect is good；Trocar sheath 1 and knob lid 3
The safeguard protection effect with freezing slide glass 2, so freezing slide glass 2 can be effectively prevented from liquid nitrogen in being contained in the sleeve pipe of sealing
Directly contact, decrease the chance of cross-contamination/infection；
Trocar sheath 1 is provided with external screw thread 11, threadeds with the knob lid 3 being provided with female thread 31, removably connects, operation
Simply, and threaded, good stability.
Freezing slide glass 2 wall is thin, transparent, it is simple to technical staff can clearly observe when operating under inverted microscope, can be fast
Speed puts into circular indicia 21 by needing freezing few sperm.Freezing slide glass 2 is provided with circular indicia 21, and volume is little, recovery
Sperm be easily found, the sperm response rate is high；It is provided with handle 23, during sperm freezing, by little tweezer on freezing slide glass 2
Sub-folder is lived freezing slide glass 2 and can be taken out from culture dish by freezing slide glass 2, convenient and practical.By freezing slide glass 2 when handle 23 uses
Insert the inner side opening sub-knob lid 33, buckle female knob lid 34, safe and reliable for freezing slide glass 2 can be buckled in knob lid 3
In be inserted in trocar sheath 1.
Knob lid bag 3 enclosed tool knob lid 33 and female knob lid 34, and sub-knob lid 33 is provided with platform 331, platform 331 sets
Having fixed groove 332, its fixed groove 332 matches with the fixing strip 22 on handle 23.Freezing slide glass 2 when band handle 23
After containing good freezing sample, can be not concerned about when putting the sub-knob lid 33 opening equivalent button into turning on one's side or reverse；So can again
Female knob lid 34 closes, and buckles sub-snap close 333 and locking device and a female 341, this is existed with sub-knob lid 33 knob of freezing slide glass 2
On trocar sheath 1.
The using method of this utility model rareness sperm freezing ultra-fine pipe carrier comprises the following steps
(1) sperm freezing
In 60mm culture dish, do 3 strips (mHTF, containing 10%SSS) drop containing balance liquid, cover mineral oil, at general
Sperm specimen after reason adds in (mHTF) drop of above-mentioned balance liquid；What a ultra-thin freezing slide glass 2 of another preparation, ultra-thin cold
Add the microdroplet of the frozen solution of 2 μ L in freezing the circular indicia 21 on slide glass 2, then place it into the training containing sperm sample
Support in ware.Culture dish is proceeded under inverted microscope, utilize micromanipulation system to capture sperm, acquisition sperm will be flutterred (less than 3
Bar/every) put to ultra-thin freezing slide glass 2 in the microdroplet of freezing liquid, clamp the handle 23 on ultra-thin freezing slide glass 2 with pincet
Take out ultra-thin freezing slide glass 2, with absorbent paper, mineral oil is blotted as far as possible.Ultra-thin freezing slide glass 2 is inserted and opens in knob lid 3
Side, buckles knob lid 3, puts trocar sheath 1, screws trocar sheath 1, is fixed in aluminum frame, first places in 4 DEG C of refrigerators 20 minutes, then
Be placed in above liquid nitrogen surface in the liquid nitrogen vapor of 5cm 5-10 minute to after freezing liquid microdroplet fully charge, put into liquid nitrogen and protect for a long time
Deposit.
Two, sperm recovery
By trocar sheath 1 after liquid nitrogen container takes out, put the white trace of trocar sheath 1 on the skin, open sub-snap close 333 He on knob lid 3
Locking device and a female 341, turns on knob lid 3, clamps ultra-thin freezing slide glass 2 with pincet and takes out in trocar sheath 1, is rapidly inserted into accurate in advance
In the micrurgy culture dish got ready (being coated with 37 DEG C of mineral oil), under inverted microscope, microneedle is utilized to capture multiple
Sperm after Soviet Union is directly used in the injection of the interior monosperm of ooecium slurry.
A kind of spiral cover bushing type rareness sperm superthin section freezing carrier of the present utility model, frozen effective, easy to operate,
Simple in construction, the sperm response rate is high, and after recovery, living spermatozoa percentage is high；It is provided with freezing slide glass 2, and freezing slide glass 2 one end sets circle
Mark 21, wall is thin, transparent, it is simple to technical staff can clearly observe when operating under inverted microscope, can be quickly cold by needs
The few sperm frozen puts into circular indicia 21；Circular indicia 21 on freezing slide glass 2, volume is 2 microlitres, and device volume is little, multiple
The sperm of Soviet Union is easily found, and the sperm response rate is high；It is provided with trocar sheath 1, can be effective in freezing slide glass 2 is contained in the sleeve pipe of sealing
Avoid the direct contact of liquid nitrogen, decrease the chance of cross-contamination/infection；Being provided with knob lid 3, its knob lid 3 is by fixing
Groove 332 is fixing connects freezing slide glass 2, and knob lid 3 is connected by female thread 31 is fixing with the external screw thread 11 on trocar sheath 1, rotation
Button lid 3 uses equivalent padlock mode, easy to use, good stability, is not concerned about turning on one's side or reverse: this practicality is new during use
A kind of spiral cover bushing type rareness sperm superthin section freezing carrier of type, uses micrurgy+microdroplet lid oil process and comes freezing dilute
Few (single) sperm, after selecting sperm end of operation under microscope, takes out carrier culture dish and removes covering on carrier again
Oil, can put in the sleeve pipe of band spiral cover, put liquid nitrogen storage；The sperm response rate is higher than 90%, recovery survival rate about 50%.It freezes
Deposit effective, easy to use, be suitable for clinical expansion and use.
The above is only preferred implementation of the present utility model, it is noted that for the common skill of the art
Art personnel, on the premise of without departing from this utility model principle, it is also possible to makes some improvement and supplements, and these improve and supplement
Also should be regarded as protection domain of the present utility model.
Claims (9)
1. a spiral cover bushing type rareness sperm superthin section freezing carrier, it is characterised in that described spiral cover bushing type rareness sperm
Superthin section freezing carrier includes trocar sheath, freezing slide glass, knob lid；Described freezing slide glass one end is fixed on knob lid, separately
One end is inserted in trocar sheath；Described trocar sheath end connects knob lid；Described trocar sheath one end is provided with external screw thread；Described overcoat
In cone inside the pipe other end: described knob lid includes sub-knob lid and female knob lid；Described sub-knob lid and female rotation
The medial surface of button lid is equipped with female thread, and female thread matches with the external screw thread on trocar sheath；Described sub-knob lid and mother
Knob lid is connected by fold mechanism；It is provided with sub-snap close outside described sub-knob lid；Female lock it is provided with outside described female knob
Button；It is additionally provided with platform on described sub-knob lid, and platform is provided with fixed groove；One end of described slide glass is additionally provided with circular mark
Know；The other end of described slide glass is additionally provided with fixing strip, handle；Described fixing strip matches with fixed groove.
Spiral cover bushing type rareness sperm superthin section freezing carrier the most according to claim 1, it is characterised in that outside described
Sleeve pipe two ends are hatch frame.
Spiral cover bushing type rareness sperm superthin section freezing carrier the most according to claim 1, it is characterised in that circular indicia
Having two, each circular indicia internal volume is 2 microlitres.
Spiral cover bushing type rareness sperm superthin section freezing carrier the most according to claim 1, it is characterised in that described slide glass
A length of 23.5 millimeters, width is 8 millimeters.
Spiral cover bushing type rareness sperm superthin section freezing carrier the most according to claim 1, it is characterised in that described overcoat
Bore be 10mm slide glass width be 8mm.
Spiral cover bushing type rareness sperm superthin section freezing carrier the most according to claim 1, it is characterised in that outside described
Sleeve pipe is prepared by heatproof high molecule material.
Spiral cover bushing type rareness sperm superthin section freezing carrier the most according to claim 1, it is characterised in that described handle
Height be 6.2 millimeters, width is 4.95 millimeters.
Spiral cover bushing type rareness sperm superthin section freezing carrier the most according to claim 1, it is characterised in that described hands
Handle is provided with triangle mark.
Spiral cover bushing type rareness sperm superthin section freezing carrier the most according to claim 1, it is characterised in that described fixing
Card article is rectangle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620669425.6U CN205794638U (en) | 2016-06-29 | 2016-06-29 | A kind of spiral cover bushing type rareness sperm superthin section freezing carrier |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620669425.6U CN205794638U (en) | 2016-06-29 | 2016-06-29 | A kind of spiral cover bushing type rareness sperm superthin section freezing carrier |
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| Publication Number | Publication Date |
|---|---|
| CN205794638U true CN205794638U (en) | 2016-12-14 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201620669425.6U Active CN205794638U (en) | 2016-06-29 | 2016-06-29 | A kind of spiral cover bushing type rareness sperm superthin section freezing carrier |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107535482A (en) * | 2017-10-11 | 2018-01-05 | 山东大学齐鲁医院 | A kind of bulk Vitrification of Ovarian Tissue carrier and its application |
| CN111700061A (en) * | 2020-06-12 | 2020-09-25 | 山东大学齐鲁医院 | Closed vitrification carrier and application thereof |
-
2016
- 2016-06-29 CN CN201620669425.6U patent/CN205794638U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107535482A (en) * | 2017-10-11 | 2018-01-05 | 山东大学齐鲁医院 | A kind of bulk Vitrification of Ovarian Tissue carrier and its application |
| CN111700061A (en) * | 2020-06-12 | 2020-09-25 | 山东大学齐鲁医院 | Closed vitrification carrier and application thereof |
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