CN205556665U - One set is used for equipment that single bacterial colony microorganism selected and inoculated - Google Patents

One set is used for equipment that single bacterial colony microorganism selected and inoculated Download PDF

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Publication number
CN205556665U
CN205556665U CN201521060460.XU CN201521060460U CN205556665U CN 205556665 U CN205556665 U CN 205556665U CN 201521060460 U CN201521060460 U CN 201521060460U CN 205556665 U CN205556665 U CN 205556665U
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China
Prior art keywords
rifle
gun
button
rifle head
inoculating
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Expired - Fee Related
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CN201521060460.XU
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Chinese (zh)
Inventor
张溪
王友权
孙露露
张衡
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Individual
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Individual
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Abstract

The utility model belongs to the experimental device field discloses one set of equipment that can be used to the microorganism inoculation, comprises inoculation rifle, inoculating needle, inoculation box and stack, the inoculation rifle includes rifle head, rifle core and gun barrel, the rifle head comprises rifle head shell, inner prop, spring and button, on the button was fixed in to inner prop upper end, the lower extreme is fluted to be used for fixed rifle core, the ring spring winds the inner prop, and on the button was fixed in to the upper end, the lower extreme was fixed in on the flange of rifle head shell inner wall, gun barrel, upper end pass through the screw thread and rifle head shell links to each other, and the lower extreme has the connector of connecting the inoculating needle, the recess on the inner prop is connected to rifle core, upper end, and the lower extreme can reciprocate along with pressing the button and stretches out or the gun barrel of retracting, the needle point surface of inoculating needle is the friction surface. The inoculation box for deposit the inoculating needle, the stack. The utility model discloses make this operation of microorganism inoculation further oversimplify, unitize and the standardization.

Description

A set of equipment selected for single bacterium colony microorganism and inoculate
Technical field
The utility model relates to a set of equipment selected for single bacterium colony microorganism and inoculate, and belongs to experiment equipment neck Territory.
Background technology
Selecting and inoculating of single bacterium colony microorganism is the common operation of Microbiological Lab, genetic engineering especially recently The rise of technology, select and cultivate have a certain specialized character recombinant bacterium be operated in day in Microbiological Lab Become common and frequent.But, in this operation, there is no the operation instrument of Unified Generalization so far, each experiment Room, according to own situation, is selected tweezers, haemostatic clamp, toothpick, wooden stick etc. to carry out simple combination and is completed.Meanwhile, Toothpick and wooden stick are wooden structures, and itself, containing the nutriment needed for a lot of growth of microorganism, chooses bacterium with it After join in culture medium, also some experimental results tested may be affected.It addition, use tweezer Son or haemostatic clamp grip toothpick then to carry out choosing the complex operation of bacterium inoculation and easily making a fault.So, invention It is a set of that to choose bacterium for single bacterium colony microorganism necessary with the equipment of inoculation.
Utility model content
In order to overcome Microbiological Lab single bacterium colony select and inoculate in operation disunity, the lacking of difficult operation Falling into, technical problem to be solved in the utility model is to provide to be selected and setting of inoculating for single bacterium colony microorganism Standby.The experimental implementation that this complete equipment can simplify and specification list bacterium colony microorganism selects and inoculates.
The technical solution adopted in the utility model is:
A set of equipment selected for single bacterium colony microorganism and inoculate, by inoculating gun, transfer needle, inoculation box and rifle Frame forms;Described inoculating gun includes rifle head, rifle core and gun barrel, and described rifle head, by rifle head shell, inner prop, bullet Spring and button form, and described inner prop upper end is fixed on button, and lower end is fluted for fixing rifle core, described bullet Circlip is around inner prop, and upper end is fixed on button, and lower end is fixed on the flange of rifle head outer casing inner wall;Described gun barrel, Upper end is connected with rifle head shell by screw thread, and there is the connector connecting transfer needle lower end;Described rifle core, upper end is even Connecing the groove on inner prop, lower end can move up and down with button and stretches out or retraction gun barrel;
Described transfer needle is made up of pintle, pin stalk and needle point, and described pintle can be nested on the connector of gun barrel; Described inoculation box, is used for depositing transfer needle, by inoculation box shell, drawing and pulling type opening and the circular hole with supporting leg Plate forms;Described gun rack, is used for depositing inoculating gun, and the cylinder stent by base with hook forms.
The button of described inoculating gun rifle head, can automatically reset under the action of the spring.
Fixed handle is also had on the rifle head shell of described inoculating gun.
The rifle core of described inoculating gun is by being difficult to microbiological contamination, can not be utilized by microorganism and can carry out at high temperature under high pressure The material (such as stainless steel, PP or PA plastics) of sterilizing is made.
Described transfer needle is by being difficult to microbiological contamination, can not be utilized by microorganism and can carry out sterilizing at high temperature under high pressure Material (such as PP or PA plastics) is made, and the surface of needle point is rubbing surface, the easiest and single bacterium colony adhesion. Tip end can not be excessively sharp or smooth, is otherwise difficult to provoke bacterium colony.
Described inoculation box, is used for placing transfer needle, and shell can stand autoclave sterilization.
Described gun rack, the cylinder stent by base with hook forms.Inoculating gun can be placed in cylinder.As Really inoculating gun is the dirtiest or needs sterilization further, can invade bubble inoculating gun with device alcohol or disinfectant in cylinder. Cylinder outer wall has the arc hook what inoculating gun fixed handle is connected mutually, is used for placing inoculating gun.
Use inoculating gun equipment of the present utility model, effectively prevent tweezers or haemostatic clamp and carry out to grip toothpick This operating defect, specification equipment used by this operation.Meanwhile, transfer needle uses can not be microorganism profit PP or PA plastics replace the wooden tooth containing the potential multiple nutriment that can be utilized by microorganism Sign so that the experimental result of some experiments is more convincing.This experiment novel product, it is possible to the most unified, simple Change and select with specification list bacterium colony microorganism and inoculate, particularly the screening of recombinant bacterium and this operation of cultivation.
The utility model can also customize the inoculating gun of different model, transfer needle according to the demand in laboratory, And provide and inoculate box accordingly, for laboratory microbial inoculant or pass on, particularly the selecting and inoculating of single bacterium colony Work, it is provided that the most more convenient instrument.
The utility model compared with prior art, has the advantage that
1, the utility model devises the detachable inoculating gun assembled being mainly used in single colony inoculation, low cost Honest and clean, simple to operate;
2, the utility model replaces the most common wooden toothpick used by inoculation, unified be difficult to by microorganism Inorganic material that degraded utilizes and can according to needing to design the transfer needle replacement containing rubbing surface in long and short tip, It is also less prone to while being easy to choosing colony be utilized by microorganism, also will not change the culture medium of some accurate experiments Nutritional labeling;
3, the utility model gun rack, design is simple, and function is more;Cylinder can contain alcohol or cleaning agent docking Planting rifle carry out disinfection and clean, cylinder stent surrounding contains hook simultaneously, can place the inoculating gun being not required to clean.
4, the cost of manufacture of the utility model all devices is relatively low, simple in construction, and the life-span is long.
Accompanying drawing explanation
Fig. 1 is the structural representation of the utility model inoculating gun.
Fig. 2 is the structural representation of the utility model transfer needle.
Fig. 3 is the structural representation of the utility model inoculation box.
Fig. 4 is the structural representation of the utility model gun rack.
Wherein, 1-rifle head shell, 2-inner prop, 3-spring, 4-button, 5-groove, 6-rifle core, 7-gun barrel, 8-connector, 9-pintle, 10-pin obstructs, and 11-needle point, 12-inoculates box shell, 13-drawing and pulling type opening, 14- Circular hole slab, 15-supporting leg, 16-base, 17-cylinder stent, 18-links up with, 19-fixed handle.
Detailed description of the invention
Below in conjunction with the accompanying drawings the technical solution of the utility model is described further, but should not be construed as this reality With novel restriction.
A set of equipment selected for single bacterium colony microorganism and inoculate, by inoculating gun, transfer needle, inoculation box and rifle Frame forms.
As it is shown in figure 1, described inoculating gun includes rifle head, rifle core 6 and gun barrel 7, described rifle head, by rifle head shell 1, Inner prop 2, spring 3 and button 4 form, and described inner prop 2 upper end is fixed on button 4, and lower end fluted 5 is for solid Determining rifle core 6, described spring 3 is around inner prop 2, and upper end is fixed on button 4, and rifle head outer casing inner wall is fixed in lower end Flange on;Described gun barrel 7, upper end is connected with rifle head shell 1 by screw thread, and there is the company connecting transfer needle lower end Interface 8;Described rifle core 6, upper end connects the groove 5 on inner prop, and lower end can move up and down with button and stretches out Or retraction gun barrel 7.
As in figure 2 it is shown, described transfer needle is made up of pintle 9, pin stalk 10 and needle point 11, described pintle 9 can be nested On the connector 8 of gun barrel 7, described needle point 11 surface is rubbing surface.
As it is shown on figure 3, described inoculation box, it is used for depositing transfer needle, by inoculation box shell 12, drawing and pulling type opening 13 Form with the circular hole slab 14 with supporting leg 15.
As shown in Figure 4, described gun rack, it is used for depositing inoculating gun, the cylinder by base 16 with hook 18 props up Frame 17 forms.
During use, described rifle core is first connected with the inner prop end groove in the button of rifle head, then rifle head and rifle Cylinder connects, and forms inoculating gun.Pressing inoculating gun rifle head button, rifle core can stretch out gun barrel.Transfer needle is placed on and connects Plant in box, firmly transfer needle is connected mutually with inoculating gun connector, after transfer needle is connected with inoculating gun, holds and connect Plant rifle transfer needle to go to provoke bacterium colony, then will transfer to above culture medium, press rifle head button so that rifle core Stretch out gun barrel, and then withstand on the pintle of transfer needle so that transfer needle departs from inoculating gun, falls in culture medium, Complete inoculation operation.
Use specific operation process of the present utility model as follows:
1, by inoculating gun each several part, it is placed with the inoculation box of transfer needle after high pressure steam sterilization, cooling, and After in gnotobasis, rifle head, rifle core and gun barrel are assembled into inoculating gun successively, open inoculation box, firmly make Inoculating gun connector is connected mutually with the port of transfer needle, composition one;
2, by the inoculating gun with transfer needle, transfer so that transfer needle, to single colony locations, carries out selecting bacterium Fall;
3, after bacterium colony sticks in transfer needle, it is transferred to above culture medium, firmly presses rifle head button, by rifle core Get, and then transfer needle is come off, fall in corresponding culture medium;
4, using new transfer needle instead, said process is carried out successively.End of operation, and can be put into gun rack by inoculating gun Go up or in cylinder.

Claims (5)

  1. The most a set of equipment selected for single bacterium colony microorganism and inoculate, by inoculating gun, transfer needle, inoculation box Form with gun rack;Described inoculating gun includes rifle head, rifle core (6) and gun barrel (7), and described rifle head, by rifle head Shell (1), post (2), spring (3) and button (4) composition, described post (2) upper end is fixed on On button (4), lower end fluted (5) are used for fixing rifle core (6), described spring (3) around post (2), Upper end is fixed on button (4), and lower end is fixed on the flange of rifle head shell (1) inwall;Described gun barrel (7), Upper end is connected with rifle head shell (1) by screw thread, and there is the connector (8) connecting transfer needle lower end;Described rifle Core (6), upper end connects the groove (5) on post, and lower end can move up and down with button and stretches out or retraction rifle Cylinder (7);
    Described transfer needle is made up of pintle (9), pin stalk (10) and needle point (11), and described pintle (9) can It is nested on the connector (8) of gun barrel (7);Described inoculation box, is used for depositing transfer needle, by inoculating outside box Shell (12), drawing and pulling type opening (13) form with the circular hole slab (14) with supporting leg (15);Described rifle Frame, is used for depositing inoculating gun, and the cylinder stent (17) by base (16) with hook (18) forms.
  2. Equipment the most according to claim 1, it is characterised in that the rifle core (5) of described inoculating gun by PP, PA or stainless steel material are made.
  3. Equipment the most according to claim 1, it is characterised in that described transfer needle is by PP or PA plastic production Form.
  4. Equipment the most according to claim 1, it is characterised in that on rifle head shell (1) of described inoculating gun Also has fixed handle (19).
  5. Equipment the most according to claim 1, it is characterised in that described needle point (11) surface is rubbing surface.
CN201521060460.XU 2015-12-13 2015-12-13 One set is used for equipment that single bacterial colony microorganism selected and inoculated Expired - Fee Related CN205556665U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201521060460.XU CN205556665U (en) 2015-12-13 2015-12-13 One set is used for equipment that single bacterial colony microorganism selected and inoculated

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201521060460.XU CN205556665U (en) 2015-12-13 2015-12-13 One set is used for equipment that single bacterial colony microorganism selected and inoculated

Publications (1)

Publication Number Publication Date
CN205556665U true CN205556665U (en) 2016-09-07

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108251287A (en) * 2018-01-22 2018-07-06 李长寿 A kind of sustainable operation type bacterial reproduction culture moves bacterium instrument

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108251287A (en) * 2018-01-22 2018-07-06 李长寿 A kind of sustainable operation type bacterial reproduction culture moves bacterium instrument
CN108251287B (en) * 2018-01-22 2021-08-13 山东拜因诺生物科技有限公司 Sustainable operation type bacterium reproduction culture bacteria transferring instrument

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C14 Grant of patent or utility model
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20160907

Termination date: 20171213

CF01 Termination of patent right due to non-payment of annual fee