CN102217544A - Open tissue culture and factory fast propagation method for potatoes - Google Patents
Open tissue culture and factory fast propagation method for potatoes Download PDFInfo
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- CN102217544A CN102217544A CN2011101346164A CN201110134616A CN102217544A CN 102217544 A CN102217544 A CN 102217544A CN 2011101346164 A CN2011101346164 A CN 2011101346164A CN 201110134616 A CN201110134616 A CN 201110134616A CN 102217544 A CN102217544 A CN 102217544A
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Abstract
The invention discloses an open tissue culture and factory fast propagation method for potatoes. A high-pressure sterilization labile round or square plastic box is used as the culture vessel, and an MS culture medium is used as a basic culture medium. The method comprises the following steps of: regulating the pH to 5.4 after the culture medium is cooked, charging the culture medium to the plastic box, and cooling and solidifying the culture medium as culture base. The method has the advantages that: high-pressure sterilization is not needed, and a large amount of culture medium preparation time is saved; meanwhile, because the culture medium is not subjected to high-pressure sterilization, the nutritional components are not lost, and the growth period of tissue culture seedlings is shortened from original 20 days to 12 days; an ultraclean workbench is not needed, inoculation is performed under the open and bacterial environment, the operation is simple, and the inoculation speed is 4 times that of the traditional mode; and when being cultured in the open and bacterial greenhouse, the growth of the tissue culture seedlings of the potatoes is robust, and the survival rate of the transplanted seedlings is high.
Description
Technical field
The present invention relates to the method for a kind of potato tissue cultivation and industrial fast breeding.
Background technology
Traditional potato tissue cultural method need carry out under aseptic condition, and operation sequence is loaded down with trivial details, pollution rate is high, inoculation speed slow, cultivate the cost height, transplanting survival rate is low
Traditional potato group training is loaded down with trivial details, and culture vessel need be selected the vial or the plastic bottle of high temperature high voltage resistant, and for avoiding polluting, the bottle type is smaller, and inoculation quantity bud generally has only about 30 strains.
Summary of the invention
The objective of the invention is to cultivate group training environmental requirement harshness in fast numerous process at current potato tissue, need under strict aseptic condition, carry out, problems such as operation sequence is loaded down with trivial details, pollution rate is high, inoculation speed slow, cultivate the cost height, transplanting survival rate is low, invent open tissue culture of a kind of potato and the batch production new method of breeding fast, potato tissue is cultivated broken away from strict aseptic operating environment, realize high efficiency, low-cost tissue culture and the batch production purpose of breeding fast in the collarium border in nature, open having.
For achieving the above object, the invention provides the method for open tissue culture of a kind of potato and industrial fast breeding, its with the circle of not high pressure resistant sterilization or square plastic box as culture vessel; With the MS medium is minimal medium, after medium is cooked, transfers PH to 5.4, divides to install in the plastic casing, and cooled and solidified is promptly as medium;
May further comprise the steps:
Fast numerous stage, transfer room alcohol depositing dust, the abundant sterilization of ultra violet lamp, the alcohol of usefulness will inoculate table top and hand is wiped clean, and opens the lid, and with scissors the potato tissue cultivating seedling is cut into 3 to 4 sections, evenly is tiled on the medium with tweezers;
Take root the stage, transfer room is with alcohol depositing dust, ultra violet lamp, and the alcohol with 75% will inoculate table top and hand is wiped clean, and opens the lid, and is cut into stem-segment with single bud with will the tile potato tissue cultivating seedling of gained of scissors, and the cuttage of usefulness tweezers is on medium; And
The potato that has connected is placed in the greenhouse, control temperature daytime and be no more than 30 ℃, be not less than 5 ℃ evening, in the incubation,, keep the culture environment of a relative clean, cultivated about 12 days, can carry out acclimatization and transplants regularly to culturing room's cleaning of sterilizing.
Beneficial effect of the present invention do not need to be autoclaving, has saved the medium preparation time in a large number, simultaneously since medium without autoclaving, the nutrient component free of losses, the tissue cultivating seedling growth cycle shortened to 12 days by original 20 days; Not by superclean bench, inoculate opening, have under the collarium border, simple to operate, inoculation speed is 4 times of traditional approach; In the greenhouse of opening, have bacterium, cultivate potato tissue cultivating seedling robust growth, hardening survival rate height.
Embodiment
1, container is selected
And in the present invention, adopt the circle of not high pressure resistant sterilization or square plastic box as culture vessel, and inoculation quantity is up to 80 strains, owing to adopt antibiotic medium, pollution rate is no more than 0.3%.
2, the preparation of medium
The present invention is a minimal medium with the MS medium, after medium is cooked, adds 0.3ml of mountain farming, transfers PH to 5.4.Install in the plastic casing with PLT-G50 medium dosing filling machine branch,, can use after the cooled and solidified without autoclaving.
3, inoculation method
(1) fast numerous stage
Transfer room is with 75% alcohol depositing dust, ultra violet lamp 20min, alcohol with 75% will be inoculated table top (desk etc. do not need superclean bench) and hand is wiped clean, and open the lid, with scissors the potato tissue cultivating seedling is cut into three-4 sections, evenly is tiled on the medium with tweezers.
(2) take root the stage
Transfer room is with 75% alcohol depositing dust, ultra violet lamp 20min, alcohol with 75% will be inoculated table top (desk etc., do not need superclean bench) and hand wipe clean, open the lid, be cut into stem-segment with single bud with will the tile potato tissue cultivating seedling of gained of scissors, with the tweezers cuttage on medium, but every box cuttage 80 strains.
4, indoor cultivation
The potato that has connected is placed in the greenhouse, control temperature daytime and be no more than 30 ℃, be not less than 5 ℃ evening, in the incubation,, keep the culture environment of a relative clean, cultivated about 12 days, can carry out acclimatization and transplants regularly to culturing room's cleaning of sterilizing.
Claims (4)
1. the method for open tissue culture of potato and industrial fast breeding is characterized in that:
With the circle of not high pressure resistant sterilization or square plastic box as culture vessel; With the MS medium is minimal medium, after medium is cooked, transfers PH to 5.4, divides to install in the plastic casing, and cooled and solidified is promptly as medium;
May further comprise the steps:
Fast numerous stage, transfer room alcohol depositing dust, the abundant sterilization of ultra violet lamp, the alcohol of usefulness will inoculate table top and hand is wiped clean, and opens the lid, and with scissors the potato tissue cultivating seedling is cut into 3 to 4 sections, evenly is tiled on the medium with tweezers;
Take root the stage, transfer room is with alcohol depositing dust, the abundant sterilization of ultra violet lamp, and the alcohol with 75% will inoculate table top and hand is wiped clean, and opens the lid, and is cut into stem-segment with single bud with will the tile potato tissue cultivating seedling of gained of scissors, and the cuttage of usefulness tweezers is on medium; And
The potato that has connected is placed in the greenhouse, control temperature daytime and be no more than 30 ℃, be not less than 5 ℃ evening, in the incubation,, keep the culture environment of a relative clean, cultivated about 12 days, can carry out acclimatization and transplants regularly to culturing room's cleaning of sterilizing.
2. method according to claim 1 is characterized in that: alcohol is 75% alcohol.
3. method according to claim 1 is characterized in that: add the mountain farming and transfer a PH to 5.4.
4. method according to claim 1 is characterized in that: ultra violet lamp sterilization in 20 minutes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011101346164A CN102217544A (en) | 2011-05-24 | 2011-05-24 | Open tissue culture and factory fast propagation method for potatoes |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011101346164A CN102217544A (en) | 2011-05-24 | 2011-05-24 | Open tissue culture and factory fast propagation method for potatoes |
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| Publication Number | Publication Date |
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| CN102217544A true CN102217544A (en) | 2011-10-19 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| CN2011101346164A Pending CN102217544A (en) | 2011-05-24 | 2011-05-24 | Open tissue culture and factory fast propagation method for potatoes |
Country Status (1)
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| CN (1) | CN102217544A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106359094A (en) * | 2016-08-31 | 2017-02-01 | 天津大学 | Method for quickly inducing potato minituber |
| CN107347419A (en) * | 2017-08-21 | 2017-11-17 | 山西省农业科学院作物科学研究所 | A kind of bud culture method for culturing seedlings of potato |
| CN109042326A (en) * | 2018-08-21 | 2018-12-21 | 中国科学院遗传与发育生物学研究所 | Plant Tissue Breeding open inoculation method |
| CN111602595A (en) * | 2020-06-28 | 2020-09-01 | 甘肃省农业科学院马铃薯研究所 | Open type rapid propagation method for potato tissue culture seedlings |
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| JP2000106777A (en) * | 1998-10-08 | 2000-04-18 | Kubota Corp | Propagation of tuberous root |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106359094A (en) * | 2016-08-31 | 2017-02-01 | 天津大学 | Method for quickly inducing potato minituber |
| CN106359094B (en) * | 2016-08-31 | 2018-07-06 | 天津大学 | A kind of method of rapid induction micro potato |
| CN107347419A (en) * | 2017-08-21 | 2017-11-17 | 山西省农业科学院作物科学研究所 | A kind of bud culture method for culturing seedlings of potato |
| CN107347419B (en) * | 2017-08-21 | 2020-03-20 | 山西省农业科学院作物科学研究所 | Bud culture seedling method for potatoes |
| CN109042326A (en) * | 2018-08-21 | 2018-12-21 | 中国科学院遗传与发育生物学研究所 | Plant Tissue Breeding open inoculation method |
| CN111602595A (en) * | 2020-06-28 | 2020-09-01 | 甘肃省农业科学院马铃薯研究所 | Open type rapid propagation method for potato tissue culture seedlings |
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Application publication date: 20111019 |