CN204918611U

CN204918611U - Cell culture bag

Info

Publication number: CN204918611U
Application number: CN201520687191.3U
Authority: CN
Inventors: 颜学恒; 张智培
Original assignee: Shanghai Xp Biomed Ltd
Current assignee: Shanghai Xp Biomed Ltd
Priority date: 2015-09-07
Filing date: 2015-09-07
Publication date: 2015-12-30
Anticipated expiration: 2025-09-07

Abstract

The utility model provides a cell culture bag, includes the bag physically, the external interval from top to bottom of bag clamp is equipped with a plurality of clamps that seal, and this bag of body passes through a plurality of seal to press from both sides separate into bag at the bottom of a plurality of expansion bag and, seal and press from both sides including preceding clamping bar and back clamping bar, the tail end of preceding clamping bar and back clamping bar is articulated, it is protruding that the front end of preceding clamping bar has the card, the rear end of back clamping bar have with the buckle of the protruding correspondence of card, ventilative mouth, sample connection, transfer line interface and inoculation cell interface has on the end bag. The utility model discloses simple structure, preparation convenience when culture cell, are selected to unload next perhaps a plurality of clamps that seal according to cell density and can be expanded the volume, need not change the container, reduce the manual operation flow by a wide margin, do not need operation technique just can let the cell keep certain density simultaneously, and the cell can not polluted to the continued growth to conveniently, the success rate is high.

Description

A kind of cell culture bags

Technical field

The utility model relates to cell culture technology, particularly relates to a kind of cell culture bags.

Background technology

Cell cultures has now been widely used in the every field such as biology, medical science, new drug development, becomes one of most important basic science.

Traditional cell culture processes, need to maintain certain cell density, therefore culture vessel volume can not be excessive, otherwise cell density is too low, cannot grow; But when cell proliferation is to some amount, exceed certain density, then too intensive cell can stop growing, even aging apoptosis.Now must change the culture vessel of larger volume, need the operative technique of extreme care, to avoid cell contamination, very inconvenient and be easy to failure.

Utility model content

Based on this, for above-mentioned technical problem, provide a kind of cell culture bags.

For solving the problems of the technologies described above, the utility model adopts following technical scheme:

A kind of cell culture bags, comprise bag, the outer spacer clip from top to bottom of described bag is provided with multiple sealing clip, this bag is separated into bag at the bottom of multiple expansion bag and by described multiple sealing clip, described sealing clip comprises front clamping bar and rear clamping bar, the tail end of described front clamping bar and rear clamping bar is hinged, the front end of described front clamping bar has card convex, the rear end of described rear clamping bar has the buckle corresponding with described card convex, bag of the described end has ventilation mouth, thief hole, tubing interface and inoculating cell interface.

Bag of the described end is funnel-form.

An expansion bag in described multiple expansion bag has ventilation mouth, thief hole, tubing interface and inoculating cell interface.

In described ventilation mouth, there is filter core.

Described expansion bag has scale marker.

The utility model structure is simple, easy to make, when culturing cell, select to unload one or more sealing clip and extendible volume according to cell density, container need not be changed, significantly reduce manual operation flow process, the density simultaneously not needing operative technique that cell just can be allowed to remain certain, continued growth, can not contamination of cells, and convenient, success ratio is high.

Accompanying drawing explanation

Below in conjunction with the drawings and specific embodiments, the utility model is described in detail:

Fig. 1 is structural representation of the present utility model;

Fig. 2 is side-looking structural representation of the present utility model;

Fig. 3 is the structural representation of sealing clip of the present utility model.

Embodiment

As shown in Figure 1 and Figure 2, a kind of cell culture bags of volume change, comprises bag 110.

The outer spacer clip from top to bottom of bag 110 is provided with multiple sealing clip 120, and this bag 110 is separated into bag 112 at the bottom of multiple expansion bag 111 and by multiple sealing clip 120.

When culturing cell, select to unload one or more sealing clip 120 i.e. extendible volume according to cell density, container need not be changed, significantly reduce manual operation flow process, the density simultaneously not needing operative technique that cell just can be allowed to remain certain, continued growth, can not contamination of cells, and convenient, success ratio is high.

Wherein, as shown in Figure 3, sealing clip 120 comprises front clamping bar 121 and rear clamping bar 122, and the tail end of front clamping bar 121 and rear clamping bar 122 is hinged, and the front end of front clamping bar 121 has card convex 123, and the rear end of rear clamping bar 122 has the buckle 124 corresponding with card convex 123.

End bag 112 has ventilation mouth 113, thief hole 114, tubing interface 115 and inoculating cell interface 116.

Preferably, in multiple expansion bag 111 one expands and bag 111 also has ventilation mouth 113, thief hole 114, tubing interface 115 and an inoculating cell interface 116.

In ventilation mouth 113, there is filter core, air free diffusing can be allowed to enter in bag 110, but other materials or bacterium can not be allowed to pass.

Thief hole 114, for sampling quality inspection, is conveniently monitored.

Tubing interface 115 can be connected with tubing, also can inject liquid by syringe.

Inoculating cell interface 116 can inoculating cell.

Preferably, expand on bag 111 and have scale marker, user correctly can know culture volume.

End bag 112, in funnel-form, can allow cell lean on bottom gravity natural subsidence to sack, reduces cell centrifugation amount, convenient operation.

But, those of ordinary skill in the art will be appreciated that, above embodiment is only used to the utility model is described, and be not used as restriction of the present utility model, as long as in spirit of the present utility model, all will drop in Claims scope of the present utility model the change of the above embodiment, modification.

Claims

1. a cell culture bags, it is characterized in that, comprise bag, the outer spacer clip from top to bottom of described bag is provided with multiple sealing clip, this bag is separated into bag at the bottom of multiple expansion bag and by described multiple sealing clip, described sealing clip comprises front clamping bar and rear clamping bar, the tail end of described front clamping bar and rear clamping bar is hinged, the front end of described front clamping bar has card convex, the rear end of described rear clamping bar has the buckle corresponding with described card convex, bag of the described end has ventilation mouth, thief hole, tubing interface and inoculating cell interface.

2. a kind of cell culture bags according to claim 1, is characterized in that, bag of the described end is funnel-form.

3. a kind of cell culture bags according to claim 1 and 2, is characterized in that, an expansion bag in described multiple expansion bag has ventilation mouth, thief hole, tubing interface and inoculating cell interface.

4. a kind of cell culture bags according to claim 3, is characterized in that, has filter core in described ventilation mouth.

5. a kind of cell culture bags according to claim 4, is characterized in that, described expansion bag has scale marker.