CN204474693U - A kind of culture apparatus special for drug treating zebrafish embryo and juvenile fish - Google Patents
A kind of culture apparatus special for drug treating zebrafish embryo and juvenile fish Download PDFInfo
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- CN204474693U CN204474693U CN201520111511.0U CN201520111511U CN204474693U CN 204474693 U CN204474693 U CN 204474693U CN 201520111511 U CN201520111511 U CN 201520111511U CN 204474693 U CN204474693 U CN 204474693U
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The utility model relates to a kind of culture apparatus special for drug treating zebrafish embryo and juvenile fish, comprises and cultivates body and intermediate; Described cultivation body comprises base plate and multiple culture tank, the uniform upper surface being arranged on base plate of described multiple culture tank; Described intermediate comprises the insertosome be connected as a single entity by multiple culture hole, and the bottom of described culture hole all arranges filtering net; After described culture hole inserts corresponding culture tank, described intermediate and cultivation body are combined into an entirety.Be drawn onto juvenile fish in the residual water process of breeding fish of absorption before this culture apparatus can avoid administration causes juvenile fish to damage by mistake, can ensure that again each culture hole drug solution volume is accurately consistent.This culture apparatus design concept is ingenious, easy to use, and effect is obviously, respond well, ensure that experiment accuracy, improves working efficiency.
Description
Technical field
The utility model relates to a kind of culture apparatus special for drug treating zebrafish embryo and juvenile fish, belongs to culture vessel technical field.
Background technology
Zebra fish (zebrafish) is a kind of low profile thermal hairtail.Because egg laying amount is large, the feature such as simple, the transparent easy observation of embryo of easily collecting, raising, now become the important model building human disease model and medicament high flux screening research biological.Its duplicated disease and human diseases have high similarity, have the features such as reliability, controllability, easy property and economy.Adopt zebra fish to carry out consumption that compound medicine efficacy screening and toxicity assessment not only can reduce tested material, can carry out Large-scale Screening simultaneously, obviously shorten research cycle, reduction research cost.
Zebra fish has so many advantage, but does not have the culture apparatus special of drug treating zebrafish embryo and juvenile fish at present.Normally zebrafish embryo or juvenile fish suction pipe are transferred in common culture plate culture hole, to wherein remove by water again, but like this cannot residual liquid in all sucking-off culture hole, cause the administration volume of each culture hole cannot be completely accurately consistent, and continuously in a couple of days administration process, the liquid operation of changing of every day easily makes embryo and juvenile fish be subject to artificial damage, causes experimental error.
Therefore, a kind of culture apparatus being exclusively used in drug treating zebrafish embryo and juvenile fish of design is needed badly.
Utility model content
For the deficiencies in the prior art, the utility model provides a kind of culture apparatus special for drug treating zebrafish embryo and juvenile fish.
The technical solution of the utility model is as follows:
For a culture apparatus special for drug treating zebrafish embryo and juvenile fish, described culture apparatus special comprises cultivates body and intermediate; Described cultivation body comprises base plate and multiple culture tank, the uniform upper surface being arranged on base plate of described multiple culture tank; Described intermediate comprises the insertosome be connected as a single entity by multiple culture hole, and the bottom of described culture hole arranges filtering net; After described culture hole inserts corresponding culture tank, described intermediate and cultivation body are combined into an entirety.
Preferably, described multiple culture tank becomes the uniform upper surface being arranged on base plate of many rows.
Preferably, the quantity of described culture tank is 1 to 192.
Preferred further, the quantity of described culture tank is 96 or 24 or 12 or 6.
Preferably, the profile of described culture tank is set to hollow cylindrical, and correspondingly, the profile of described culture hole is also set to hollow cylindrical.
Preferably, described culture apparatus special also comprises plate lid, and described plate lid is arranged on the upper surface of intermediate in order to seal culture hole.
Preferably, the surrounding of described base plate is provided with the outer lower than culture tank notch; The surrounding of described plate lid is provided with the outer to downward-extension, and when after plate lid sealing culture hole, the described outer of plate lid contacts with the outer of base plate to coordinate to be formed and seals entirety.
Preferably, described intermediate is provided with the edge extended to surrounding.The advantage of this design is, the edge increasing design facilitates picking and placeing of intermediate.
Preferably, the specification of described filtering net is 40-90 order.
Preferred further, the specification of described filtering net is 60 orders.
During use, assembled mutually with cultivation body by insert intermediate and coordinate, the filtering net arranged bottom culture hole is in the bottom of culture tank; When changing liquid, only need mention insert intermediate, the separation with culture plate plate body and original fluid of embryo or juvenile fish can be realized, avoid in same culture hole, draw nutrient solution mistiming and be drawn onto embryo or juvenile fish, remove the injury to embryo or juvenile fish from; Insert intermediate surrounding is provided with outer rim, can realize closely cooperating of insert intermediate and culture plate plate body, be convenient to picking and placeing of insert intermediate simultaneously.
The beneficial effects of the utility model are:
The culture apparatus special of the utility model zebrafish embryo and juvenile fish, when changing liquid, only need mention insert intermediate, the separation with culture plate plate body and original fluid of embryo or juvenile fish can be realized, avoid in same culture hole, draw nutrient solution mistiming and be drawn onto embryo or juvenile fish, remove the injury to embryo or juvenile fish from; Meanwhile, the administration volume specification of each culture tank is identical, is drawn onto juvenile fish by mistake causes juvenile fish to damage, can ensure that again each culture hole drug solution volume is accurately consistent before can avoiding administration like this in the residual water process of breeding fish of absorption.This culture apparatus design concept is ingenious, easy to use, and effect is obviously, respond well, ensure that experiment accuracy, improves working efficiency.
Accompanying drawing explanation
Fig. 1 is the structural representation that in the utility model, body is cultivated in 6 holes;
Fig. 2 is the structural representation of the intermediate cultivating mates in the utility model with 6 holes;
Fig. 3 is the structural representation of the plate lid cultivating mates in the utility model with 6 holes;
Fig. 4 is the assembling schematic diagram that the utility model cultivates when body, intermediate and Ban Gai match.
Wherein: 1, cultivate body, 101, culture tank, 102, outer, 2, intermediate, 201, culture hole, 202, filtering net, 203, edge, 3, Ban Gai, 301, outer.
Embodiment
Also by reference to the accompanying drawings the utility model is described further below by embodiment, but is not limited thereto.
Embodiment 1:
As shown in Figures 1 to 4, for 6 hole culture apparatuses, the present embodiment provides a kind of culture apparatus special for drug treating zebrafish embryo and juvenile fish, comprises and cultivates body 1 and intermediate 2; Described cultivation body 1 comprises base plate and 6 culture tanks 101, the uniform upper surface being arranged on base plate of described 6 culture tanks; Described intermediate 2 comprises the insertosome be connected as a single entity by 6 culture hole 201, and the bottom of described culture hole 201 arranges filtering net 202; After described culture hole 201 inserts corresponding culture tank 101, described intermediate 2 and cultivation body 1 are combined into an entirety.
Cultivate body 1 and be provided with 6 flat circular culture tanks 101, the internal diameter of culture tank 101 is 34-35mm, and the degree of depth is 17-18mm.Insert intermediate 2 arranges 6 the flat circular culture hole 201 corresponding with cultivating body, and culture hole 201 volume is slightly smaller than culture tank 101, ensures that insert intermediate 2 is set in together with cultivation body 1 close fit.Keep a fixed gap bottom culture hole 201 and culture tank 101, ensure the circulation of cultivating water.Insert intermediate 2 surrounding is provided with edge 203, is convenient to picking and placeing of insert intermediate.Bottom insert intermediate culture hole 201, mesh-like filtering net 202 is set, ensure cultivate water quickly through and zebrafish embryo and juvenile fish cannot pass through.The culture hole 201 surrounding material of insert intermediate is identical with cultivation body 1, and bottom filtering net 202 material is formal wire or nylon wire, and specification is 60 orders, aperture 0.3mm.
The culture tank 101 6 holes being cultivated body 1 (cultivating body 1A) corresponding with insert intermediate 2 is set in together with culture hole 201 wall close fit, selects normotrophic zebra fish juvenile fish, is moved in insert intermediate 2 culture hole 201.Drug solution is joined another hole to cultivate in body (cultivating body 1B).Taken out from cultivation body 1A by insert intermediate containing zebra fish juvenile fish, former water of breeding fish is flowed out automatically by water vent, is inserted in the cultivation body 1B of drug containing solution by the insert culture plate intermediate only containing zebra fish juvenile fish, is put in constant-temperature incubation in illumination box.Be drawn onto juvenile fish in the residual water process of breeding fish of absorption before can avoiding administration like this causes juvenile fish to damage by mistake, can ensure that again each culture hole drug solution volume is accurately consistent.
Embodiment 2:
The present embodiment provides a kind of culture apparatus special for drug treating zebrafish embryo and juvenile fish, structure as described in Example 1, its difference is: in order to increase stopping property when this culture apparatus special uses, prevent the pollution being subject to external environment, this culture apparatus special also comprises a plate lid 3.The surrounding of the base plate of cultivation body 1 is provided with the outer 102 lower than culture tank 101 notch.The surrounding of plate lid 3 is provided with the outer 301 to downward-extension, and after plate lid 3 seals culture hole 201, the outer 301 of plate lid 3 contacts with the outer 102 of base plate and coordinates formation entirety sealed.The advantage of this design is, when after plate lid sealing culture hole, the outer of plate lid closely cooperates with the outer of cultivating body, form the space of a sealing, make culture tank be in a relatively airtight clean environment, from the pollution of external environment, ensure the accuracy of experimental result.
Embodiment 3:
The present embodiment provides a kind of culture apparatus special for drug treating zebrafish embryo and juvenile fish, structure as described in Example 2, its difference is: cultivation body 1 and insert intermediate 2 are provided with 12 culture tanks 101 and 12 culture hole 201, the internal diameter of culture tank 101 is 21-22mm, the degree of depth is 14-15mm, and filtering net 202 specification is 40 orders.
Embodiment 4:
The present embodiment provides a kind of culture apparatus special for drug treating zebrafish embryo and juvenile fish, structure as described in Example 2, its difference is: cultivation body 1 and insert intermediate 2 are provided with 24 culture tanks 101 and 24 culture hole 201, the internal diameter of culture tank 101 is 15-16mm, the degree of depth is 13-14mm, and filtering net 202 specification is 90 orders.
Embodiment 5:
The present embodiment provides a kind of culture apparatus special for drug treating zebrafish embryo and juvenile fish, structure as described in Example 2, its difference is: cultivation body 1 and insert intermediate 2 are provided with 96 culture tanks 101 and 96 culture hole 201, the internal diameter of culture tank 101 is 6-7mm, the degree of depth is 10-11mm, and filtering net 202 specification is 60 orders.
Embodiment 6:
The present embodiment provides a kind of culture apparatus special for drug treating zebrafish embryo and juvenile fish, and as described in Example 2, its difference is structure: cultivate body 1 and insert intermediate 2 is provided with 192 culture tanks 101 and 192 culture hole 201.
Claims (10)
1. for a culture apparatus special for drug treating zebrafish embryo and juvenile fish, it is characterized in that, described culture apparatus special comprises cultivates body and intermediate; Described cultivation body comprises base plate and multiple culture tank, the uniform upper surface being arranged on base plate of described multiple culture tank; Described intermediate comprises the insertosome be connected as a single entity by multiple culture hole, and the bottom of described culture hole arranges filtering net; After described culture hole inserts corresponding culture tank, described intermediate and cultivation body are combined into an entirety.
2. as claimed in claim 1 for the culture apparatus special of drug treating zebrafish embryo with juvenile fish, it is characterized in that, described multiple culture tank becomes and arranges the uniform upper surface being arranged on base plate.
3., as claimed in claim 1 or 2 for the culture apparatus special of drug treating zebrafish embryo and juvenile fish, it is characterized in that, the quantity of described culture tank is 1 to 192.
4., as claimed in claim 3 for the culture apparatus special of drug treating zebrafish embryo and juvenile fish, it is characterized in that, the quantity of described culture tank is 96 or 24 or 12 or 6.
5., as claimed in claim 1 or 2 for the culture apparatus special of drug treating zebrafish embryo and juvenile fish, it is characterized in that, the profile of described culture tank is set to hollow cylindrical, and correspondingly, the profile of described culture hole is also set to hollow cylindrical.
6., as claimed in claim 1 for the culture apparatus special of drug treating zebrafish embryo and juvenile fish, it is characterized in that, described culture apparatus special also comprises plate lid, and described plate lid is arranged on the upper surface of intermediate in order to seal culture hole.
7., as claimed in claim 6 for the culture apparatus special of drug treating zebrafish embryo and juvenile fish, it is characterized in that, the surrounding of described base plate is provided with the outer lower than culture tank notch; The surrounding of described plate lid is provided with the outer to downward-extension, and when after plate lid sealing culture hole, the described outer of plate lid contacts with the outer of base plate to coordinate to be formed and seals entirety.
8., as claimed in claim 1 for the culture apparatus special of drug treating zebrafish embryo with juvenile fish, it is characterized in that, described intermediate is provided with the edge to surrounding extension.
9., as claimed in claim 1 or 2 for the culture apparatus special of drug treating zebrafish embryo and juvenile fish, it is characterized in that, the specification of described filtering net is 40-90 order.
10., as claimed in claim 9 for the culture apparatus special of drug treating zebrafish embryo and juvenile fish, it is characterized in that, the specification of described filtering net is 60 orders.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201520111511.0U CN204474693U (en) | 2015-02-16 | 2015-02-16 | A kind of culture apparatus special for drug treating zebrafish embryo and juvenile fish |
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| CN201520111511.0U CN204474693U (en) | 2015-02-16 | 2015-02-16 | A kind of culture apparatus special for drug treating zebrafish embryo and juvenile fish |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106855560A (en) * | 2017-02-28 | 2017-06-16 | 山东省科学院生物研究所 | The device and its method of work of a kind of Fast Evaluation compound acute toxicity |
| CN107966581A (en) * | 2017-11-03 | 2018-04-27 | 同济大学 | A kind of zebrafish embryo microwell plate arranges adding set |
| CN108982826A (en) * | 2018-09-28 | 2018-12-11 | 广东工业大学 | Test device and test method of a kind of zebra fish detection nano particle to embryotoxicity |
| CN109197732A (en) * | 2018-09-12 | 2019-01-15 | 杭州电子科技大学 | It is a kind of for changing the container of liquid to zebrafish embryo in aquarium or juvenile fish |
| CN109220912A (en) * | 2018-09-28 | 2019-01-18 | 广东工业大学 | A kind of zebra fish conservation cultural method |
| CN110537997A (en) * | 2018-05-29 | 2019-12-06 | 山东省科学院生物研究所 | Special device for tumor transplantation of zebra fish juvenile fish and working method |
| CN111394246A (en) * | 2020-03-26 | 2020-07-10 | 浙江省农业科学院 | Fish embryo collecting and culturing device and method for evaluating toxicity of thiamethoxam and tetraconazole combined effect |
| CN111849772A (en) * | 2020-07-31 | 2020-10-30 | 中国科学院生态环境研究中心 | Device and method for continuous culture and observation of model fish embryos |
-
2015
- 2015-02-16 CN CN201520111511.0U patent/CN204474693U/en active Active
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106855560A (en) * | 2017-02-28 | 2017-06-16 | 山东省科学院生物研究所 | The device and its method of work of a kind of Fast Evaluation compound acute toxicity |
| CN106855560B (en) * | 2017-02-28 | 2023-07-21 | 山东省科学院生物研究所 | Device for rapidly evaluating acute toxicity of compound and working method thereof |
| CN107966581A (en) * | 2017-11-03 | 2018-04-27 | 同济大学 | A kind of zebrafish embryo microwell plate arranges adding set |
| CN107966581B (en) * | 2017-11-03 | 2023-08-29 | 同济大学 | Zebra fish embryo microplate arrangement adding device |
| CN110537997A (en) * | 2018-05-29 | 2019-12-06 | 山东省科学院生物研究所 | Special device for tumor transplantation of zebra fish juvenile fish and working method |
| CN109197732A (en) * | 2018-09-12 | 2019-01-15 | 杭州电子科技大学 | It is a kind of for changing the container of liquid to zebrafish embryo in aquarium or juvenile fish |
| CN108982826A (en) * | 2018-09-28 | 2018-12-11 | 广东工业大学 | Test device and test method of a kind of zebra fish detection nano particle to embryotoxicity |
| CN109220912A (en) * | 2018-09-28 | 2019-01-18 | 广东工业大学 | A kind of zebra fish conservation cultural method |
| CN108982826B (en) * | 2018-09-28 | 2024-03-26 | 广东工业大学 | Test device and test method for detecting embryotoxicity of nano particles by zebra fish |
| CN111394246A (en) * | 2020-03-26 | 2020-07-10 | 浙江省农业科学院 | Fish embryo collecting and culturing device and method for evaluating toxicity of thiamethoxam and tetraconazole combined effect |
| CN111849772A (en) * | 2020-07-31 | 2020-10-30 | 中国科学院生态环境研究中心 | Device and method for continuous culture and observation of model fish embryos |
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