Composite PCR plate
Technical field:
The utility model relates to the anti-application tool technical field of PCR, refers in particular to a kind of composite PCR plate.
Background technology:
At present, in molecular biology research and medical clinic applications process, PCR reaction technology is indispensable.PCR reaction and polymerase chain reaction (polymerase chainreaction, PCR), be a kind of method of specific gene of rapid amplifying in vitro or DNA sequence dna, therefore be also called the amplification in vitro method of gene.At DNA segment both sides to be amplified and two Oligonucleolide primers with its both sides complementation, after several circulations of sex change, annealing and extension, DNA cloning 2 square time doubly.In order to batch amplification, commonly use PCR Sptting plate in PCR reaction, PCR Sptting plate is generally 8 holes, 12 holes, 96 holes or 384 holes, is specially for batch reaction designs, for PCR instrument and sequenator.
Existing PCR plate includes stereotype plate that PC material makes and the tube sheet that PP material is made, it is split-type structural between stereotype plate and tube sheet, tube sheet is arranged on stereotype plate upper surface, tube sheet is provided with some holes, stereotype plate is provided with the salient point coordinated with hole, be fixed by the wringing fit between salient point and hole between tube sheet and stereotype plate, but this fixed sturcture more complicated, assembling trouble, production efficiency is low, and little in conjunction with contact surface between tube sheet and stereotype plate, in conjunction with built on the sand, likely there is skew in use procedure between tube sheet and stereotype plate or rock, affect experimental result.
Utility model content:
The purpose of this utility model be exactly for prior art exist deficiency and the composite PCR plate that a kind of tube sheet is combined firmly with base, structure is simple, production efficiency is high is provided.
To achieve these goals, the technical solution adopted in the utility model is: composite PCR plate, it includes base, the tube sheet be made up of PP material, base is made up of the hard plastics not identical with tube sheet, tube sheet forms multiple closed bottom downwards, open-topped reaction body, base includes panel, around the carriage being arranged on faceplate perimeter lower end, tube sheet is arranged on the panel of base, panel offers respectively with multiple reaction bodys open holes one to one, between tube sheet with base by the one-body molded mode of double-material injection-molding in conjunction with formation integral structure.
Described base is made up of PC or nylon plastic material.
Preferably, described base is made up of glass fibre reinforced nylon composite plastic or glass fiber reinforcement PC composite plastic.
Bonding surface between described tube sheet and the panel of base is plane.
Described tube sheet is arranged on the lower surface of panel.
The mouth of pipe upper surface of described reaction body is concordant with tube sheet upper surface, and the spout diameter of reaction body is consistent with open holes diameter.
The mouth of pipe of described reaction body also can protrude out tube sheet upper surface, and protrudes out panel upper surface through corresponding open holes, and the mouth of pipe external diameter of reaction body is consistent with open holes diameter.
Described tube sheet also can be arranged on the upper surface of panel, and each reaction body is plugged in corresponding open holes respectively, and the mouth of pipe of reaction body protrudes out tube sheet upper surface.
Described reaction body is pyramidal structure, and open holes is the tapered hole with reaction body corresponding matching.
Gap is left between described reaction tubes external surface and open holes.
The utility model beneficial effect is: the composite PCR plate that the utility model provides, it includes base, the tube sheet be made up of PP material, base is made up of the hard plastics not identical with tube sheet, tube sheet forms multiple closed bottom downwards, open-topped reaction body, base includes panel, around the carriage being arranged on faceplate perimeter lower end, tube sheet is arranged on the panel of base, panel offers respectively with multiple reaction bodys open holes one to one, between tube sheet with base by the one-body molded mode of double-material injection-molding in conjunction with formation integral structure, make to combine between tube sheet and base tightr, more firm, there will not be skew between tube sheet and base or rock, ensure the accuracy of experimental result, and do not need salient point and hole to coordinate between tube sheet with base to be fixed, integrated structure is simple, tube sheet and base is formed successively by carrying out the one-body molded mode of double-material injection-molding in one set of die, simplification of flowsheet, without the need to assembling between tube sheet and base, greatly improve working (machining) efficiency.
Accompanying drawing illustrates:
Fig. 1 is structural representation of the present utility model.
Fig. 2 is decomposition texture schematic diagram of the present utility model.
Fig. 3 is the cut-away view of the utility model reaction body.
Embodiment:
Below in conjunction with accompanying drawing, the utility model is further described, see shown in Fig. 1 ~ 3, the utility model includes base 1, the tube sheet 2 be made up of PP material, base 1 is made up of the hard plastics not identical with tube sheet 2, tube sheet 2 forms multiple closed bottom downwards, open-topped reaction body 21, base 1 includes panel 11, around the carriage 12 being arranged on panel 11 periphery lower end, tube sheet 2 is arranged on the panel 11 of base 1, panel 11 offers respectively with multiple reaction body 21 open holes 13 one to one, between tube sheet 2 with base 1 by the one-body molded mode of double-material injection-molding in conjunction with formation integral structure.
Base 1 can be made up of PC or nylon plastic material, and particularly, can be made up of glass fibre reinforced nylon composite plastic or glass fiber reinforcement PC composite plastic, intensity is higher, and dimensional stability is better.Bonding surface between the panel 11 of tube sheet 2 and base 1 is plane, and integrated structure is simple, and increase the bonding surface area between tube sheet 2 and the panel 11 of base 1, structure is more firm.
In the present embodiment, tube sheet 2 is arranged on the upper surface of panel 11, and each reaction body 21 is plugged in corresponding open holes 13 respectively.Reaction body 21 be pyramidal structure, and open holes 13 be and the tapered hole of reaction body 21 corresponding matching, and making to react when body 21 is put in open holes 13 can automatic aligning, accurate positioning, in conjunction with firmly.The mouth of pipe 22 of reaction body 21 protrudes out tube sheet 2 upper surface, prevents the liquid of tube sheet 2 upper surface from entering the mouth of pipe 22 of reaction body 21, avoids the sample reacting body 21 contaminated.Leave gap between reaction body 21 outside surface and open holes 13, prevent the performance of the Effect of Materials reaction body 21 of base 1, more can the accuracy of warranty test result.
Between the utility model tube sheet 2 with base 1 by the one-body molded mode of double-material injection-molding in conjunction with formation integral structure, make to combine more closely between tube sheet 2 and base 1, more firm, there will not be between tube sheet 2 and base 1 and offset or rock, ensure the accuracy of experimental result, and integrated structure is simple between tube sheet 2 and base 1, tube sheet 2 and base 1 is formed successively by carrying out the one-body molded mode of double-material injection-molding in one set of die, simplification of flowsheet, without the need to assembling between tube sheet 2 and base 1, greatly improve working (machining) efficiency.
As another embodiment of the present utility model, tube sheet 2 is arranged on the lower surface of panel 11.The mouth of pipe 22 of reaction body 21 protrudes out tube sheet 2 upper surface, and protrude out panel 11 upper surface through corresponding open holes 13, prevent the liquid on panel 11 from entering the mouth of pipe 22 of reaction body 21, avoid the sample reacting body 21 contaminated, the mouth of pipe 22 external diameter of reaction body 21 is consistent with open holes 13 diameter, combines and form integrative-structure between the mouth of pipe 22 of reaction body 21 and open holes 13.
Certainly, the mouth of pipe 22 upper surface of reaction body 21 also can be concordant with tube sheet 2 upper surface, and the mouth of pipe 22 internal diameter of reaction body 21 is consistent with open holes 13 diameter, and open holes 13 also form a part for the mouth of pipe 22 of reaction body 21, and structure is simpler.
Certainly, the above is only better embodiment of the present utility model, therefore all equivalences done according to structure, feature and the principle described in the utility model patent claim change or modify, and are included in the utility model patent claim.