CN204848844U - PCR test tube and array thereof - Google Patents
PCR test tube and array thereof Download PDFInfo
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- CN204848844U CN204848844U CN201520388241.8U CN201520388241U CN204848844U CN 204848844 U CN204848844 U CN 204848844U CN 201520388241 U CN201520388241 U CN 201520388241U CN 204848844 U CN204848844 U CN 204848844U
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- test tube
- tube wall
- pcr
- pcr test
- utility
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- 238000012360 testing method Methods 0.000 title claims abstract description 55
- 239000012780 transparent material Substances 0.000 claims abstract description 7
- 239000000463 material Substances 0.000 claims description 16
- 238000000034 method Methods 0.000 claims description 11
- 239000004743 Polypropylene Substances 0.000 claims description 6
- 239000011521 glass Substances 0.000 claims description 6
- 239000007769 metal material Substances 0.000 claims description 6
- 238000000465 moulding Methods 0.000 claims description 6
- 229920003023 plastic Polymers 0.000 claims description 6
- 229920001155 polypropylene Polymers 0.000 claims description 6
- 238000000338 in vitro Methods 0.000 abstract description 10
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 3
- 238000002474 experimental method Methods 0.000 abstract description 3
- 238000011065 in-situ storage Methods 0.000 abstract 1
- 238000006243 chemical reaction Methods 0.000 description 12
- 230000003321 amplification Effects 0.000 description 4
- 238000001816 cooling Methods 0.000 description 4
- 238000003199 nucleic acid amplification method Methods 0.000 description 4
- 241000973497 Siphonognathus argyrophanes Species 0.000 description 3
- 108020004414 DNA Proteins 0.000 description 2
- 108091028043 Nucleic acid sequence Proteins 0.000 description 2
- 238000012408 PCR amplification Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 238000003752 polymerase chain reaction Methods 0.000 description 2
- 238000010792 warming Methods 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 238000012300 Sequence Analysis Methods 0.000 description 1
- NIXOWILDQLNWCW-UHFFFAOYSA-N acrylic acid group Chemical group C(C=C)(=O)O NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 230000003292 diminished effect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
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- 239000000203 mixture Substances 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 150000007523 nucleic acids Chemical group 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
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- Devices For Use In Laboratory Experiments (AREA)
Abstract
The utility model discloses a PCR test tube and array thereof, including one end open -ended pipe wall with set up in window on the pipe wall, the window is the transparent material window. The utility model discloses the high thermal conductivity not only has, moreover can in situ observation to the experiment condition of in vitro reagent or gather in vitro signal, convenient to use.
Description
Technical field
The utility model relates to the experimental installation of biological technical field, particularly relate to a kind of thermal conductivity high, be easy to observe in vitro situation or gather PCR test tube and the array thereof of in vitro signal.
Background technology
At present, in biology field, PCR reaction is conventional experimental technique, PCR reaction and polymerase chain reaction (PolymeraseChainReaction, PCR) are a kind of methods of external enzyme' s catalysis specific DNA fragment, reacted by a few step such as high-temperature denatured, low-temperature annealing and thermophilic extension and form one-period, circulation is carried out, and makes target DNA be able to rapid amplification, has high specificity, highly sensitive, easy and simple to handle, the feature such as save time.It not only can be used for the fundamental researchs such as gene isolation, clone and nucleic acid sequence analysis, also can be used for the diagnosis of disease.Round pcr is also known as cell-free molecular cloning or the directed enzymatic amplification technique of the external primer of specific DNA sequences.
PCR test tube is the reaction vessels generally used during pcr amplification reaction is implemented, a typical pcr amplification reaction has been come the heating and cooling that the PCR test tube be placed on wherein carries out repeatedly by PCR thermal cycler, in this process, the conduction of temperature passes to by PCR thermal cycler heatable metal block the PCR test tube be placed on wherein again, then the tube wall through PCR test tube conducts heat to the PCR reaction solution in pipe, thus causes amplified reaction.Whether a PCR reaction can rapidly and efficiently increase except the composition of netic module quality, polysaccharase character, reaction solution to netic module, and the speed of the heating and cooling of reaction solution is also key factor.The efficiency that the faster PCR of warming and cooling rate reacts is higher, more not easily produces non-specific amplification product, and warming and cooling rate is fast, greatly can shorten the time of PCR reaction.
At present general in the world PCR test tube (amplification pipe; thin-walled tube, reaction tubes) the general high-quality clear acrylic that adopts is made; be usually divided into single tube, eight connecting legs and 96 orifice plates (8 × 12); but only have about 0.2W/mK, poor heat conduction due to polyacrylic thermal conductivity, and adopt Shooting Technique to make; and tube wall is the thinnest can only accomplish 0.25-0.30mm; in whole PCR temperature cycle process, test tube tube wall becomes maximum thermal resistance, is the bottleneck of heat transfer; Roche Holding Ag once produced glass thin-walled kapillary, although transparent, thermal conductivity good, due to very easily broken, use inconvenience, add that its cost is close to 20 times of plastic test tube, seldom employs at present.
Utility model content
For the defect of above-mentioned prior art and the technical problem of existence, the primary technical problem that the utility model solves is to provide a kind of PCR test tube and array thereof, not only there is high thermal conductivity, and can Real Time Observation in vitro reagent or gather the in vitro experimental conditions such as signal.
The technical scheme realizing above-mentioned purpose is:
A kind of PCR test tube that the utility model provides, the tube wall comprising one end open and the form be arranged on tube wall, form is transparent material form.
Further improvement of the utility model is, form is extended to bottom tube wall by tube wall top.
Further improvement of the utility model is, form is arranged at lower part of tube wall.
Further improvement of the utility model is, form is transparent plastics material form or glass material form.
Further improvement of the utility model is, tube wall is modified polypropene material tube wall or metal material tube wall.
Further improvement of the utility model is, tube wall is pyramid type.
Further improvement of the utility model is, tube wall is ladder type.
Further improvement of the utility model is, PCR test tube is the test tube made by two-shot molding process.
The another aspect of the technical program, a kind of PCR cuvette array, this PCR cuvette array comprises multiple above-mentioned PCR test tube.
The utility model is owing to have employed above technical scheme, and making it have following beneficial effect is:
1, the tube wall in the utility model adopts modified polypropene material tube wall or the metal material tube wall of high thermal conductivity coefficient, and form is offered on tube wall, compared with prior art, not only thermal resistance is little for the PCR test tube that the technical program provides, good heat conduction effect, and user by the form of transparent material can Real Time Observation in vitro reagent or gather the in vitro test situation such as signal, have easy to use, substantially increase the advantage of PCR experiment efficiency.
2, the PCR test tube that the technical program provides adopts two-shot molding process to make, thus decreases production cost, is beneficial to and promotes the use of.
3, the PCR test tube that provides of the technical program, form according to actual needs, can be opened in the optional position on tube wall, has more practicality.
Accompanying drawing explanation
Fig. 1 is embodiment one structural representation of a kind of PCR test tube of the utility model;
Fig. 2 is embodiment two structural representation of a kind of PCR test tube of the utility model;
Fig. 3 is embodiment three structural representation of a kind of PCR test tube of the utility model;
Fig. 4 is embodiment three lateral plan of a kind of PCR test tube of the utility model;
Fig. 5 is embodiment three three-dimensional view of a kind of PCR test tube of the utility model.
Embodiment
Below in conjunction with the accompanying drawing in the utility model embodiment, be clearly and completely described the technical scheme in the utility model embodiment, obviously, described embodiment is only the utility model part embodiment, instead of whole embodiments.Based on the embodiment in the utility model, those of ordinary skill in the art are not making the every other embodiment obtained under creative work prerequisite, all belong to the scope of the utility model protection.
Embodiment one
As shown in Figure 1, a kind of PCR test tube, the tube wall 1 comprising one end open and the form 2 be arranged on tube wall 1, form 2 is transparent material form.
Optimal way of the present utility model is, PCR test tube is the test tube made by two-shot molding process, tube wall 1 is pyramid type, and the diameter of tube wall 1 diminishes gradually by bottom test tube mouth to test tube, and tube wall 1 is metal material tube wall or thermal conductivity is not less than the modified polypropene material tube wall of 0.7W/mK.
Form 2 is transparent plastics material form or glass material form, and this form 2 is the window of circle, rectangle or other arbitrary shapes, and form 2 is opened in lower part of tube wall 1, and form 2 is one or more.
Embodiment two
As shown in Figure 2, a kind of PCR test tube, the tube wall 1 comprising one end open and the form 2 be arranged on tube wall 1, form 2 is transparent material form.
Optimal way of the present utility model is, PCR test tube is the test tube made by two-shot molding process, tube wall 1 is pyramid type, and the diameter of tube wall 1 diminishes gradually by bottom test tube mouth to test tube, and tube wall 1 is metal material tube wall or thermal conductivity is not less than the modified polypropene material tube wall of 0.7W/mK.
Form 2 is transparent plastics material form or glass material form, and form 2 extends to the bottom of tube wall 1 by the top of tube wall 1 always, and user can by this form 2 Real Time Observation to the situation of test tube interior or gather in vitro signal etc.
Embodiment three
As shown in Figures 3 to 5, a kind of PCR cuvette array, this PCR cuvette array 3 comprises the multiple PCR test tubes be set up in parallel, and the tube wall 1 that this PCR test tube comprises one end open and the form 2 be arranged on tube wall, form 2 is transparent material form.
Particularly, PCR test tube is the test tube made by two-shot molding process, tube wall 1 for cross section be rectangle by the trapezoidal-structure diminished gradually at the bottom of test tube mouth to test tube, tube wall 1 is overall in flats in the direction of the width, and tube wall 1 is the modified polypropene material tube wall that metal material tube wall or thermal conductivity are not less than 0.7W/mK.
Form 2 is transparent plastics material form or glass material form, form 2 extends to the bottom of tube wall 1 by the top of tube wall 1 always, and the form 2 of all test tubes is unified is arranged at PCR cuvette array 3 side outwardly, user can by this form 2 Real Time Observation to the situation of all test tube interior or gather in vitro signal etc.
Should be appreciated that the noun of locality in the technical program all the regular job custom of binding operation person and Figure of description set up, their appearance should not affect protection domain of the present utility model.
Below embodiment has been described in detail the utility model by reference to the accompanying drawings, and those skilled in the art can make many variations example to the utility model according to the above description.Thus, some details in embodiment should not formed restriction of the present utility model, the utility model by the scope that defines using appended claims as protection domain of the present utility model.
Claims (9)
1. a PCR test tube, is characterized in that: the tube wall comprising one end open and the form be arranged on described tube wall, described form is transparent material form.
2. a kind of PCR test tube according to claim 1, is characterized in that: described form is extended to bottom described tube wall by described tube wall top.
3. a kind of PCR test tube according to claim 1, is characterized in that: described form is arranged at lower part of described tube wall.
4. according to a kind of PCR test tube in claim 1-3 described in any one, it is characterized in that: described form is transparent plastics material form or glass material form.
5. according to a kind of PCR test tube in claim 1-3 described in any one, it is characterized in that: described tube wall is modified polypropene material tube wall or metal material tube wall.
6. a kind of PCR test tube according to claim 1, is characterized in that: described tube wall is pyramid type.
7. a kind of PCR test tube according to claim 1, is characterized in that: described tube wall is ladder type.
8. a kind of PCR test tube according to claim 1, is characterized in that: described PCR test tube is the test tube made by two-shot molding process.
9. a PCR cuvette array, is characterized in that: this PCR cuvette array comprises multiple PCR test tube as claimed in any of claims 1 to 8 in one of claims.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520388241.8U CN204848844U (en) | 2015-06-05 | 2015-06-05 | PCR test tube and array thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520388241.8U CN204848844U (en) | 2015-06-05 | 2015-06-05 | PCR test tube and array thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204848844U true CN204848844U (en) | 2015-12-09 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520388241.8U Active CN204848844U (en) | 2015-06-05 | 2015-06-05 | PCR test tube and array thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN204848844U (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106701560A (en) * | 2016-06-13 | 2017-05-24 | 秦荣 | PCR test tube |
| CN106867892A (en) * | 2017-01-09 | 2017-06-20 | 杭州安杰思生物科技有限公司 | A kind of metal test tubes PCR instrument |
| CN108913811A (en) * | 2018-07-05 | 2018-11-30 | 湖南圣湘生物科技有限公司 | The rapid amplifying method of hepatitis B virus nucleic acid |
| CN116814397A (en) * | 2023-08-25 | 2023-09-29 | 新羿制造科技（北京）有限公司 | PCR reaction tube and multi-row PCR tube |
-
2015
- 2015-06-05 CN CN201520388241.8U patent/CN204848844U/en active Active
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106701560A (en) * | 2016-06-13 | 2017-05-24 | 秦荣 | PCR test tube |
| CN106867892A (en) * | 2017-01-09 | 2017-06-20 | 杭州安杰思生物科技有限公司 | A kind of metal test tubes PCR instrument |
| CN108913811A (en) * | 2018-07-05 | 2018-11-30 | 湖南圣湘生物科技有限公司 | The rapid amplifying method of hepatitis B virus nucleic acid |
| CN108913811B (en) * | 2018-07-05 | 2020-06-16 | 圣湘生物科技股份有限公司 | Rapid amplification method of hepatitis B virus nucleic acid |
| CN116814397A (en) * | 2023-08-25 | 2023-09-29 | 新羿制造科技（北京）有限公司 | PCR reaction tube and multi-row PCR tube |
| CN116814397B (en) * | 2023-08-25 | 2023-11-28 | 新羿制造科技（北京）有限公司 | PCR reaction tube and multi-row PCR tube |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20190729 Address after: Five Floors of Building 1181 Zhaoxian Road, Jiading District, Shanghai, 201821 Patentee after: Shanghai Hong Shi Medical Technology Co Ltd Address before: 200072 Shanghai, Zhabei District Guang Yan Road, Lane 52, room 4, No. 501 Patentee before: Qin Rong |