CN203794896U - Fermentation tank inoculation device - Google Patents
Fermentation tank inoculation device Download PDFInfo
- Publication number
- CN203794896U CN203794896U CN201420133570.3U CN201420133570U CN203794896U CN 203794896 U CN203794896 U CN 203794896U CN 201420133570 U CN201420133570 U CN 201420133570U CN 203794896 U CN203794896 U CN 203794896U
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- CN
- China
- Prior art keywords
- inoculation
- welding
- weldering seat
- fermentation tank
- bottle
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- 238000011081 inoculation Methods 0.000 title claims abstract description 78
- 238000000855 fermentation Methods 0.000 title claims abstract description 17
- 230000004151 fermentation Effects 0.000 title claims abstract description 17
- 238000003466 welding Methods 0.000 claims abstract description 29
- 229920001971 elastomer Polymers 0.000 claims abstract description 17
- 230000004308 accommodation Effects 0.000 claims abstract description 8
- 230000002572 peristaltic effect Effects 0.000 claims abstract description 8
- 229920001296 polysiloxane Polymers 0.000 claims description 15
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 12
- 229910002027 silica gel Inorganic materials 0.000 claims description 12
- 239000000741 silica gel Substances 0.000 claims description 12
- 229960001866 silicon dioxide Drugs 0.000 claims description 12
- 230000001580 bacterial effect Effects 0.000 claims description 4
- 239000007788 liquid Substances 0.000 abstract description 4
- 229920002379 silicone rubber Polymers 0.000 abstract 5
- 239000004945 silicone rubber Substances 0.000 abstract 5
- 230000001737 promoting effect Effects 0.000 abstract 1
- 230000001954 sterilising effect Effects 0.000 description 5
- 238000000034 method Methods 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000011169 microbiological contamination Methods 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 235000009161 Espostoa lanata Nutrition 0.000 description 1
- 240000001624 Espostoa lanata Species 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 230000000712 assembly Effects 0.000 description 1
- 238000000429 assembly Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000011017 operating method Methods 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to a fermentation tank inoculation device. The fermentation tank inoculation device comprises an inoculation welding base used for an inoculation bottle and welded on the fermentation tank and a silicone rubber tube for connecting the inoculation bottle and the inoculation welding base, an inoculation bottle plug is mounted at the bottleneck of the inoculation bottle, two holes are formed in the inoculation bottle plug, a liquid guide pipe is inserted into one hole, the other hole is connected to the outlet end of an air filter; the liquid guide pipe is tightly connected with one end of the silicone rubber tube, an inoculation needle is mounted on the other end of the silicone rubber tube, a peristaltic pump for promoting the flow of liquid is arranged on the silicone rubber tube; the inoculation welding base comprises a welding inner ring and a welding outer ring, the top edge of the welding inner ring is lower than the welding outer ring to form an accommodation space, the accommodation space is internally provided with a silicone rubber pad and a welding base plug; the welding base plug comprises a welding base plug sleeve and a rubber inner core, and a through hole into which the rubber inner core and the inoculation needle are inserted is arranged on the welding base plug sleeve. The inoculation device disclosed by the utility model has the advantages of simple structure, convenience in application and strong reliability, the sterile inoculation can be achieved and the inoculation device has good application prospects.
Description
Technical field
The utility model relates to a kind of classification inoculation apparatus, specifically a kind of inoculating device of fermentation tank.
Background technology
Use aborning; when the microorganism seed after activation is seeded to fermentor tank; conventionally adopt pyrosphere protection inoculation; working specification is conventionally as follows: 1, prepare: smooth fast for guaranteeing inoculation operating process; need 4 people's cooperation complete operations, a people to be responsible for opening inoculation and emit and control pyrosphere, a people is responsible for kind; one people is responsible for transmitting kind of a bottle, and a people is responsible for the control of air inlet and exhaust.Operator wear mouth mask, head-shield, fall to plant with transmission personnel and need use cotton ball soaked in alcohol wiping both hands.Confirm to shut around window, fan etc., tank week is accessible, and the parameters such as tank temperature, pH meet processing requirement, and blank is planted tank and planted the aseptic qualified of bottle, just can carry out subsequent operations.2, inoculation: pyrosphere dips in full alcohol, puts inoculation and emits, and slightly unscrews inoculation and emit.Close air and enter pot valve, close rapidly large exhaust simultaneously, open inoculation mouthful.Treat that inoculation mouth gives vent to anger very littlely, tank pressure is down to below 0.01MPa, closes inoculation mouthful, lights pyrosphere, turns on slightly inoculation and emits, and is placed in flame top.Kind of personnel, on flame ring side after tampon is slightly pulled up, burn bottleneck 3-5 second on flame, pour kind of liquid into kind of a tank rapidly, and bottleneck does not leave flame scope.Operating process will be sought to stable, standard, fast.After kind bottle graft kind completes, inoculation is emitted and on flame, burnt 3-5 second, tighten rapidly, remove pyrosphere, and put out pyrosphere with wet towel.Drive rapidly air into, then adjust exhaust, regulate air flow quantity, mixing speed, tank pressure to processing range.
From above inoculation operating method, can find out, pyrosphere seeded process needs multiple person cooperational, and coordinate and will get well, the details should be noted that is a lot, operator are had relatively high expectations, and risky link is more, if misoperation can increase microbiological contamination and dye the risk of phage, increase and pour in down a chimney rate, cause directly and indirect economic loss.
Utility model content
The utility model object is the deficiency for solving the problems of the technologies described above, and a kind of inoculating device of fermentation tank is provided, and it has light and handy convenience, can move freely, and effectively guarantees aseptic inoculation, greatly reduces the advantages such as microbiological contamination risk.
The utility model is to solve the problems of the technologies described above adopted technical scheme to be: a kind of inoculating device of fermentation tank, comprise inoculation bottle for holding bacterial classification, be welded on inoculation weldering seat on fermentor tank with for being connected the silicone tube of inoculation bottle and inoculation weldering seat, the bottleneck of described inoculation bottle is provided with inoculation bottle plug, on inoculation bottle plug, offer two holes, in one of them hole, be inserted with catheter, another hole is connected with the exit end of air filter; One end of catheter and silicone tube is tightly connected, and on the other end of silicone tube, inoculating needle is housed, and silicone tube is provided with the peristaltic pump that promotes liquid-flow; Described inoculation weldering seat is comprised of ring and welding outer shroud in the welding being welded on fermentor tank inoculation mouth, in welding, the upper edge of ring is lower than welding outer shroud, form spatial accommodation, in spatial accommodation, be provided with and cover the silicagel pad of ring upper port in welding and be pressed in the weldering seat plug in silicagel pad; Described weldering seat plug forms by welding a plug cover and rubber inner core, and described weldering seat plug puts and is provided with for inserting the through hole of rubber inner core or inoculating needle.
Described weldering seat plug cover inwall and rubber inner core top layer are equipped with screw thread, and rubber inner core can be utilized and be threaded into weldering seat plug cover.
The top layer of described inoculating needle is provided with screw thread, can utilize and be threaded into weldering seat plug cover.
Silicagel pad described in this practicality is T-shaped.
Beneficial effect is:
1, the utility model inoculating device of fermentation tank can lightly facilitate, move freely, and as required, can put at any time the sterilizing of high-temperature sterilization pot; Can shift bacterial classification at Bechtop, if once inoculate the unsuccessful secondary inoculation mobility strong that facilitates; Inoculum size can be conveniently controlled in the use of peristaltic pump.
2, the utility model classification inoculation apparatus, simple in structure, easy to use, simple to operate, single can completing inoculated operation, and in seeded process, need not change any state in fermentor tank, and risk link is few, reliability is strong, can reach aseptic inoculation, has good application prospect.
Accompanying drawing explanation
Fig. 1 is the utility model inoculating device of fermentation tank structural representation;
Fig. 2 is the inoculation weldering holder structure schematic diagram of the utility model classification inoculation apparatus;
Fig. 3 is the structural representation that weldering seat plug in seat is welded in the inoculation of the utility model classification inoculation apparatus;
Fig. 4 is the structural representation that weldering seat plug cover in seat is welded in the inoculation of the utility model classification inoculation apparatus;
Fig. 5 is the inoculating needle structural representation of the utility model classification inoculation apparatus;
In figure, mark is: 1, inoculation bottle, 11, inoculation bottle plug, 12, catheter, 13, air filter, 2, inoculation weldering seat, 21, silicagel pad, 22, ring in weldering seat plug 23, welding, 24, welding outer shroud, 25, rubber inner core, 26 weldering seat plug covers, 3, silicone tube, 4, peristaltic pump, 5, inoculating needle, 6, fermentor tank.
Embodiment
As Figure 1-5, a kind of inoculating device of fermentation tank of the utility model, comprise inoculation bottle 1 for holding bacterial classification, be welded on inoculation weldering seat 2 on fermentor tank 6 with for being connected the silicone tube 3 of inoculation bottle 1 and inoculation weldering seat 2, the bottleneck of described inoculation bottle 1 is provided with inoculation bottle plug 11, on inoculation bottle plug 11, offer two holes, in one of them hole, be inserted with catheter 12, another hole is connected with the exit end of air filter 13; Catheter 12 is tightly connected with one end of silicone tube 3, and inoculating needle 5 is housed on the other end of silicone tube 3, and silicone tube 3 is provided with the peristaltic pump 4 that promotes liquid-flow; Described inoculation weldering seat 2 forms with welding outer shroud 24 by encircling 23 in the welding being welded on fermentor tank 6 inoculation mouths, in welding, the upper edge of ring 23 is lower than welding outer shroud 24, form spatial accommodation, in spatial accommodation, be provided with and cover the silicagel pad 21 of ring 23 upper port in welding and be pressed in the weldering seat plug 22 in silicagel pad 21; Described weldering seat plug 22 is comprised of weldering seat plug cover 26 and rubber inner core 25, and described weldering seat plug cover 26 is provided with for inserting the through hole of rubber inner core 25 or inoculating needle 5.
Described weldering seat plug overlaps 26 inwalls and rubber inner core 25 top layers are equipped with screw thread, and rubber inner core 25 can be utilized and be threaded into weldering seat plug cover 26.
The top layer of described inoculating needle 5 is provided with screw thread, can utilize and be threaded into weldering seat plug cover 26.
Silicagel pad 21 described in this practicality is T-shaped.
The utility model inoculating device of fermentation tank, in use, other assemblies by inoculating device of fermentation tank except inoculation weldering seat 2 are all put into after Autoclave sterilizing, seed liquor are put into inoculation bottle 1 in Bechtop, then the rubber inner core 25 in the weldering seat plug cover 26 in inoculation weldering seat 2 is taken out, by silicagel pad 21 and inoculation weldering seat 2 interior external application alcohol swab cleaning disinfections, gauze on the good inoculating needle 5 of sterilizing is punctured to silicagel pad 21 immediately after opening insert inoculation mouthful, then will on inoculating needle 5, be screwed in inoculation weldering seat 2, inoculating needle 5 and tank body inoculation mouthful are combined closely, finally sterilizing is crossed to silicone tube 3 and connect peristaltic pump 4, by peristaltic pump 4, the seed liquor in inoculation bottle 1 is driven in fermentor tank 6 through silicone tube 3, seed liquor is extracted inoculating needle 5 after having beaten, again rubber inner core 25 is inserted to weldering seat plug cover 26, single can completing inoculated operation, and microbiological contamination risk is little.
Claims (4)
1. an inoculating device of fermentation tank, it is characterized in that: comprise inoculation bottle (1) for holding bacterial classification, be welded on inoculation weldering seat (2) on fermentor tank (6) and for being connected the silicone tube (3) of inoculation bottle (1) and inoculation weldering seat (2), the bottleneck of described inoculation bottle (1) is provided with inoculation bottle plug (11), on inoculation bottle plug (11), offer two holes, in one of them hole, be inserted with catheter (12), another hole is connected with the exit end of air filter (13); Catheter (12) is tightly connected with one end of silicone tube (3), and inoculating needle (5) is housed on the other end of silicone tube (3), and silicone tube (3) is provided with the peristaltic pump (4) that promotes liquid-flow; Described inoculation weldering seat (2) is comprised of ring (23) and welding outer shroud (24) in the welding being welded on fermentor tank (6) inoculation mouth, in welding, the upper edge of ring (23) is lower than welding outer shroud (24), form spatial accommodation, in spatial accommodation, be provided with and cover the silicagel pad (21) of ring (23) upper port in welding and be pressed in the weldering seat plug (22) in silicagel pad (21); Described weldering seat plug (22) is comprised of weldering seat plug cover (26) and rubber inner core (25), and described weldering seat plug cover (26) is provided with for inserting the through hole of rubber inner core (25) or inoculating needle (5).
2. inoculating device of fermentation tank as claimed in claim 1, is characterized in that: described weldering seat plug cover (26) inwall and rubber inner core (25) top layer are equipped with screw thread, and rubber inner core (25) can be utilized and be threaded into weldering seat plug cover (26).
3. inoculating device of fermentation tank as claimed in claim 1, is characterized in that: the top layer of described inoculating needle (5) is provided with screw thread, can utilize and be threaded into weldering seat plug cover (26).
4. inoculating device of fermentation tank as claimed in claim 1, is characterized in that: described silicagel pad (21) is T-shaped.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420133570.3U CN203794896U (en) | 2014-03-24 | 2014-03-24 | Fermentation tank inoculation device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420133570.3U CN203794896U (en) | 2014-03-24 | 2014-03-24 | Fermentation tank inoculation device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203794896U true CN203794896U (en) | 2014-08-27 |
Family
ID=51377129
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420133570.3U Expired - Fee Related CN203794896U (en) | 2014-03-24 | 2014-03-24 | Fermentation tank inoculation device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203794896U (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105145121A (en) * | 2015-10-16 | 2015-12-16 | 中国科学院天津工业生物技术研究所 | Inoculating device for liquid strains for mushroom cultivating |
| CN105176820A (en) * | 2015-09-15 | 2015-12-23 | 上海雪榕生物科技股份有限公司 | Liquid strain propagation mode |
| CN105660187A (en) * | 2016-02-23 | 2016-06-15 | 张家港顺泰元生物科技有限公司 | Aseptic silkworm cordyceps militaris strain inoculation system and method |
| CN106244435A (en) * | 2016-09-27 | 2016-12-21 | 新疆惠森生物技术有限公司 | A kind of classification inoculation apparatus for microbial manure liquid fermentation and manufacture method thereof |
| CN107216996A (en) * | 2017-05-25 | 2017-09-29 | 天方药业有限公司 | A kind of measurable classification inoculation apparatus of seeding tank and the measurable inoculation method for fermentation |
| CN108384708A (en) * | 2018-05-23 | 2018-08-10 | 宁夏西麒麟生物科技有限公司 | A kind of sterile controlled amounts type classification inoculation apparatus of seeding tank and the sterile controlled amounts type inoculation method for fermenting or cultivating |
| CN110447471A (en) * | 2019-07-31 | 2019-11-15 | 江南大学 | Plant cultivation device is used in a kind of experiment of blade inoculation endophyte of plant |
-
2014
- 2014-03-24 CN CN201420133570.3U patent/CN203794896U/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105176820A (en) * | 2015-09-15 | 2015-12-23 | 上海雪榕生物科技股份有限公司 | Liquid strain propagation mode |
| CN105145121A (en) * | 2015-10-16 | 2015-12-16 | 中国科学院天津工业生物技术研究所 | Inoculating device for liquid strains for mushroom cultivating |
| CN105660187A (en) * | 2016-02-23 | 2016-06-15 | 张家港顺泰元生物科技有限公司 | Aseptic silkworm cordyceps militaris strain inoculation system and method |
| CN106244435A (en) * | 2016-09-27 | 2016-12-21 | 新疆惠森生物技术有限公司 | A kind of classification inoculation apparatus for microbial manure liquid fermentation and manufacture method thereof |
| CN106244435B (en) * | 2016-09-27 | 2018-09-28 | 新疆惠森生物技术有限公司 | A kind of classification inoculation apparatus and its manufacturing method for microbial manure liquid fermentation |
| CN107216996A (en) * | 2017-05-25 | 2017-09-29 | 天方药业有限公司 | A kind of measurable classification inoculation apparatus of seeding tank and the measurable inoculation method for fermentation |
| CN108384708A (en) * | 2018-05-23 | 2018-08-10 | 宁夏西麒麟生物科技有限公司 | A kind of sterile controlled amounts type classification inoculation apparatus of seeding tank and the sterile controlled amounts type inoculation method for fermenting or cultivating |
| CN110447471A (en) * | 2019-07-31 | 2019-11-15 | 江南大学 | Plant cultivation device is used in a kind of experiment of blade inoculation endophyte of plant |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140827 |