CN106244435B - A kind of classification inoculation apparatus and its manufacturing method for microbial manure liquid fermentation - Google Patents
A kind of classification inoculation apparatus and its manufacturing method for microbial manure liquid fermentation Download PDFInfo
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- CN106244435B CN106244435B CN201610855494.0A CN201610855494A CN106244435B CN 106244435 B CN106244435 B CN 106244435B CN 201610855494 A CN201610855494 A CN 201610855494A CN 106244435 B CN106244435 B CN 106244435B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/12—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by pressure
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
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Abstract
The present invention discloses a kind of classification inoculation apparatus and its manufacturing method for microbial manure liquid fermentation, including bottle, bottleneck, bottle cap and conduit, and bottle uses 304 stainless steels, at triangular pyramidal, double-layer interlayer structure, bottom of bottle diameter 208mm 290mm, bottle wall height 393mm 465mm；Bottleneck is provided with external screw thread, bottle cap is provided with internal thread, bottle cap is arranged to double-layer interlayer structure, the holes 10mm are opened in bottle cap center, bottle neck passes through bottle cap central openings and welds together with bottle cap, an outer diameter 10mm air inlet pipe is welded beside bottle neck, air inlet pipe is led to across bottle cap in bottle, bottle neck upper end is provided with silicone tube, and silicone tube other end is provided with quick-joint valve；The metallic connection parts position is all using argon arc welding welding.Design is scientific and reasonable for the device, and safe operation is feasible, easy to clean, can effectively prevent miscellaneous bacteria infection, has wide applicability to liquid fermentation inoculation field.
Description
Technical field
The invention mainly relates to classification inoculation apparatus fields, specifically, the present invention relates to the classification inoculation apparatus fields of liquid fermentation.
Background technology
Liquid spawn growth cycle is short, and growth is vigorous, and energetic, production cost is low, has mobility, is sent out convenient for multiple spot
Bacterium is not easy pollution microbes, therefore liquid spawn is compared with solid spawn with apparent advantage, is the trend of Spawn incubation.But
It is to be unfavorable for Cord blood because fermenter volume is larger, therefore just need the processes such as to shift and transport.
China's microbial manure production starts from the early 1950s, having more than 60 years history, centre to experienced several so far
Developing stage.
The 50-70 ages 20th century of first stage-.Microbial manure produces based on rhizobium, to produce by indigenous method masses' fortune
Based on dynamic, based on opened type culture tank, the problem of being inoculated with using inoculation bottle is not present during this period.
The 80-90 ages 20th century of second stage-.Microbial manure is in addition to nitragin, also other bacterium microbial inoculums.It is pure
Kind culture and liquid deep layer fermenting technology start it is prevailing, utilize inoculation bottle inoculation technique occur.Technology in advance mainly utilizes glass
Strain is directly poured into seeding tank by glass triangular flask by pyrosphere sterile working by inoculation mouth, the disadvantage is that can not accomplish stricti jurise
On sterile working, inoculum concentration is small, inoculation mouth for a long time exposure, microbiological contamination is difficult to avoid.The prior art uses pressure difference culture transferring technology,
It is poor using instantaneous pressure between seeding tank and inoculation bottle using upper and lower mouth bottle,suction by pyrosphere aseptic technique, it will be inoculated with
Strain in bottle is pressed into seeding tank.The disadvantage is that the upper and lower opening silica gel plug and bottle,suction itself of bottle,suction can not be combined into effective system
One can cause strain run, drip, leak in inoculation bottle in bottle when pressurization, and microbiological contamination risk is big, and glass material bearing capacity
Difference has bottle explosion point danger, and there are security risks.
2000 phase IIIs-- are so far.Innovation and development stage of the microbial manure production into industry.Inoculation bottle changes
With stainless steel inoculation bottle, wink between seeding tank and inoculation bottle is utilized using pressure difference culture transferring technology by pyrosphere aseptic technique
Between pressure difference, by inoculation bottle strain be pressed into seeding tank.Although there is great progress, partial extent is solved to be often inoculated with
In bottle a series of production safety problems such as strain run, drip, leak in inoculation bottle can be caused when pressurization.But its unreasonable, nothing of design
The shortcomings of method thoroughly cleans still exists, and microbiological contamination point danger can not still prevent.
Invention content
For prior art defect, this patent provide a kind of classification inoculation apparatus for microbial manure liquid fermentation and its
Manufacturing method, principle are that solve the problems, such as that inoculation bottle is easy itself microbiological contamination, using argon arc by the comprehensive sterilizing of vapor
Welding technique, 304 stainless steels and unique dimension scale solve the problems, such as that inoculation bottle is difficult to clean and bearing capacity is poor,
Unique Design of Screw Thread and ultraviolet commit genocide solve the problems, such as the microbiological contamination not easy to clean and easy of difficult sealing, bottle cap.
A kind of classification inoculation apparatus for microbial manure liquid fermentation provided by the invention, including bottle, bottleneck, bottle cap and
Silicone tube, bottle use the double-layer interlayer structure of 304 stainless steels, bottle inner wall and bottle outer wall to constitute bottle interlayer knot
Structure, bottle include bottle wall and bottom of bottle two parts, and bottom of bottle and bottle wall are welded into triangular pyramidal, and there are one go out the setting of bottle top side
Inside one air expansion bottle interlayer body of vapor quick-joint valve, there are one the settings of the bottle lower part other side into one air expansion bottle of vapor quick-joint valve
Inside interlayer body, bottom of bottle diameter 208-290mm, bottle wall height 393-465mm；Bottleneck is welded on above bottle, is arranged outside bottleneck
There is an external screw thread, external screw thread is provided with multi-thread helix, bottleneck outer diameter 45-63.5mm, bottleneck height 50- using triangular tooth-shaped
60mm；It is provided with internal thread in bottle cap, threaded line is provided with multi start thread line, the interior spiral shell being arranged on the inside of bottle cap at triangular tooth-shaped
Line is adapted setting with the external screw thread being arranged on the outside of bottleneck, and bottle cap constitutes double-layer interlayer structure, bottle cap outer one by interior external bottle cap wall
Side setting is connected to bottle cap interlayer there are one vapor quick-joint valve two is gone out, and another side lower part of bottle cap is provided with one and is connect soon into vapor
Valve two is connected to bottle cap interlayer；The holes 10mm are opened in bottle cap center, bottle neck passes through bottle cap central openings and welds together with bottle cap, bottle
Mouth uses 304 stainless-steel pipes of outer diameter 10mm, bottle neck bottle neck length 40-50mm on bottom of bottle 3-10mm, bottle cap, by bottle neck
An outer diameter 10mm air inlet pipe is welded on side, and air inlet pipe is led to across bottle cap in bottle, and air inlet pipe uses 304 stainless steel tubes；On bottle neck
End is provided with silicone tube, and silicone tube other end is provided with quick-joint valve；The metallic connection parts position is all using argon arc welding welding.
In the present invention, closure inside is provided with one layer of tempered glass, leads between tempered glass and bottle cap, bottle neck and air inlet pipe
High-temperature plastic sealing is crossed, LED ultraviolet lamps are provided between glass and bottle cap.
In the present invention, heat safe rubber sleeve is provided with outside the quick-joint valve of silica gel pipe end.
In the present invention, bottle and bottle cap stainless steel thickness are 2mm-3mm.
In the present invention, bottom of bottle diameter 208mm, bottle wall height 393mm.
In the present invention, bottleneck outer diameter 45mm, bottleneck height 50mm.
In the present invention, stainless steel lathe dog is equipped with outside each quick-joint valve.
In the present invention, bottom of bottle is suppressed using tablet or the stainless steel flat bottom cover of spinning.
In the present invention, bottle wall is using the compacting welding of taper veneer reeling machine.
Meanwhile the present invention provides a kind of classification inoculation apparatus production method for microbial manure liquid fermentation, it is specific to make
Preparation Method is as follows：
（1）Bottom of bottle is made to make：One outer bottom of bottle and one is made using the stainless steel flat bottom cover of tablet compacting or spinning
A interior bottom of bottle.
（2）Bottle wall makes：Using two sets of taper veneer reeling machine compacting welding, respectively bottle inner wall and bottle outer wall.
（3）Bottleneck makes：Using the common external screw thread of cylinder, screw thread is triangular tooth-shaped, and multi-thread helix is fabricated to outer spiral shell
The bottleneck of line, then process a L-type bottleneck with cylinder stainless steel.
（4）Bottle outer wall, bottle inner wall and two kinds of bottleneck argon arc welding weldings will be processed, composition one is with outer line silk bottle
There are bottle interlayer in mouth triangular interlayer stainless steel device, centre, successively connect interior bottom of bottle and outer blank back welding, and it is outer to form a band
Line silk bottleneck triangular interlayer stainless steel triangular flask.
（5）Bottle cap makes：It selects internal thread pipe cap as bottle cap inner wall, triangular tooth-shaped, multi-thread helix, then makes one
Cylinder is as bottle cap wall, by the stainless steel ring plate argon arc welding welding with female pipe cap, cylinder and one fixed width one
It rises, forms the bottle cap with bottle cap interlayer, the hole of diameter 10mm is opened in bottle cap center, by the 304 stainless steel phials of outer diameter 10mm
Mouth passes through bottle cap argon arc welding welding, and the eyelet welding for opening a 10mm again beside bottle neck (34) taps into tracheae.
Line is drawn bottle cap punching outside bottle cap by more optimized LED ultraviolet lamps bonding in bottle cap (3), ultraviolet in LED
Tempered glass is installed under lamp, high-temperature plastic is used in combination to seal.
Implement to obtain following advantageous effect provided by the present invention for the classification inoculation apparatus of microbial manure liquid fermentation：
A kind of classification inoculation apparatus for microbial manure liquid fermentation provided by the invention, design is scientific and reasonable, transfer side
Just quick, safe operation is feasible, easy to clean, can effectively prevent miscellaneous bacteria infection, has to liquid fermentation inoculation field extensive
Applicability.
Description of the drawings
Fig. 1 is shown as classification inoculation apparatus structural schematic diagram of the present invention.
Fig. 2 is shown as bottle cross-sectional view in classification inoculation apparatus of the present invention.
Fig. 3 is shown as bottle cap cross-sectional view in classification inoculation apparatus of the present invention.
Fig. 4 is shown as carrying ultraviolet-sterilization bottle cap cross-sectional view in classification inoculation apparatus of the present invention.
Fig. 5 is shown as yoke configurations schematic diagram in classification inoculation apparatus of the present invention.
Fig. 6 is shown as clip cross section structure schematic diagram in classification inoculation apparatus of the present invention.
In figs. 1-6,1- bottles, 11- into vapor quick-joint valve one, 12- go out vapor quick-joint valve one, 13- bottles interlayer,
14- bottles inner wall, 15- bottles outer wall, 2- bottlenecks, 21- external screw threads, 3- bottle caps, 31- are steamed into vapor quick-joint valve two, 32- water outlets
Gas quick-joint valve two, 33- air inlet pipe, 34- bottle necks, 35- internal threads, 36- bottle caps interlayer, 37- bottle caps wall, 38- tempered glass, 39-
LED ultraviolet lamps, 4- silicone tubes, 41- quick-joint valves, 5- clips, 51- screw screw rod.
Specific implementation mode
1-6 and embodiment below in conjunction with the accompanying drawings are described in further detail the specific implementation mode of the present invention, but this hair
Bright device is not limited to following embodiments.
In the present invention, for ease of description, in the classification inoculation apparatus for microbial manure liquid fermentation, each component
The description of relative position relation is the Butut mode of 1-6 to be with reference to the accompanying drawings described, such as：It closes the positions such as upper and lower, left and right
System is the Butut direction of foundation attached drawing 1 to determine.
Embodiment one：
A kind of classification inoculation apparatus for microbial manure liquid fermentation provided by the invention, including bottle (1), bottleneck (2),
Bottle cap (3) and silicone tube (4), bottle (1) use the double-layer interlayer structure of 304 stainless steels, bottle inner wall（14）And bottle
Outer wall（15）Bottle interlayer (13) structure is constituted, bottle (1) includes bottle wall and bottom of bottle two parts, and bottom of bottle and bottle wall are welded into triangle
Taper, the setting of bottle (1) top side is internal there are one vapor quick-joint valve one (12) air expansion bottle interlayer body (13) is gone out, under bottle
There are one internal, bottom of bottle diameter 208mm, bottle walls into vapor quick-joint valve one (11) air expansion bottle interlayer body (13) for the setting of the portion other side
Height 393mm；Bottleneck (2) is welded on bottle (1) above, and external screw thread (21) is provided with outside bottleneck (2), and external screw thread (21) uses three
Angle thread form is provided with multi-thread helix, bottleneck (2) outer diameter 45mm, bottleneck (2) height 50mm；It is provided with internal thread in bottle cap (3)
(35), threaded line is provided with multi start thread line, bottle cap at triangular tooth-shaped（3）The internal thread of inside setting（35）With bottleneck（2）Outside
The external screw thread of side setting（21）It is adapted setting, bottle cap (3) is by interior external bottle cap wall（37）Constitute double-layer interlayer structure, bottle cap (3)
Outer side setting is provided with there are one vapor quick-joint valve two (32) connection bottle cap interlayer (36), bottle cap (3) another side lower part is gone out
One is connected to bottle cap interlayer (36) into vapor quick-joint valve two (31)；The holes 10mm are opened in bottle cap (3) center, bottle neck (34) passes through bottle
Lid (3) central openings simultaneously weld together with bottle cap (3), and bottle neck (34) uses 304 stainless-steel pipes of outer diameter 10mm, bottle neck
(34) an outer diameter 10mm air inlet pipe is welded on bottle neck (34) the length 40mm on bottom of bottle 3mm, bottle cap (3), bottle neck (34) side
(33), air inlet pipe (33) is led to across bottle cap (3) in bottle, and air inlet pipe (33) uses 304 stainless steel tubes；Bottle neck (34) upper end is arranged
There are silicone tube (4), silicone tube (4) other end to be provided with quick-joint valve (41)；The metallic connection parts position is all using argon arc welding
Welding.
In the present invention, heat safe rubber sleeve is provided with outside the quick-joint valve (41) of silicone tube (4) end.
In the present invention, the stainless steel thickness of bottle (1) and bottle cap (3) is 2mm.
Embodiment two：
A kind of classification inoculation apparatus for microbial manure liquid fermentation provided by the invention, including bottle (1), bottleneck (2),
Bottle cap (3) and silicone tube (4), bottle (1) use the double-layer interlayer structure of 304 stainless steels, bottle inner wall（14）And bottle
Outer wall（15）Bottle interlayer (13) structure is constituted, bottle (1) includes bottle wall and bottom of bottle two parts, and bottom of bottle and bottle wall are welded into triangle
Taper, for the setting of bottle (1) top side there are one vapor quick-joint valve one (12) air expansion bottle interlayer body (13) is gone out, bottle lower part is another
There are one side settings into vapor quick-joint valve one (11) air expansion bottle interlayer body (13), bottom of bottle diameter 290mm, bottle wall height
465mm；Bottleneck (2) is welded on bottle (1) above, and external screw thread (21) is provided with outside bottleneck (2), and external screw thread (21) uses trigonodont
Shape is provided with multi-thread helix, bottleneck (2) outer diameter 63.5mm, bottleneck (2) height 60mm；It is provided with internal thread in bottle cap (3)
(35), threaded line is provided with multi start thread line, bottle cap at triangular tooth-shaped（3）The internal thread of inside setting（35）With bottleneck（2）Outside
The external screw thread of side setting（21）It is adapted setting, bottle cap (3) is by interior external bottle cap wall（37）Constitute double-layer interlayer structure, bottle cap (3)
There are one vapor quick-joint valve two (32) connection bottle cap interlayer (36) is gone out, bottle cap (3) lower part other side is provided with for outer side setting
One is connected to bottle cap interlayer (36) into vapor quick-joint valve two (31)；The holes 10mm are opened in bottle cap (3) center, bottle neck (34) passes through bottle
Lid (3) central openings simultaneously weld together with bottle cap (3), and bottle neck (34) uses 304 stainless-steel pipes of outer diameter 10mm, bottle neck
(34) an outer diameter 10mm air inlet pipe is welded on bottle neck (34) the length 50mm on bottom of bottle 10mm, bottle cap (3), bottle neck (34) side
(33), air inlet pipe (33) is led to across bottle cap (3) in bottle, and air inlet pipe (33) uses 304 stainless steel tubes；Bottle neck (34) upper end is arranged
There are silicone tube (4), silicone tube (4) other end to be provided with quick-joint valve (41)；The metallic connection parts position is all using argon arc welding
Welding.
In the present invention, heat safe rubber sleeve is provided with outside the quick-joint valve (41) of silicone tube (4) end.
In the present invention, the stainless steel thickness of bottle (1) and bottle cap (3) is 3mm.
Embodiment three：
A kind of classification inoculation apparatus for microbial manure liquid fermentation provided by the invention, including bottle (1), bottleneck (2),
Bottle cap (3) and silicone tube (4), bottle (1) use the double-layer interlayer structure of 304 stainless steels, bottle inner wall（14）And bottle
Outer wall（15）Bottle interlayer (13) structure is constituted, bottle (1) includes bottle wall and bottom of bottle two parts, and bottom of bottle and bottle wall are welded into triangle
Taper, the setting of bottle (1) top side is internal there are one vapor quick-joint valve one (12) air expansion bottle interlayer body (13) is gone out, under bottle
There are one internal, bottom of bottle diameter 250mm, bottle walls into vapor quick-joint valve one (11) air expansion bottle interlayer body (13) for the setting of the portion other side
Height 450mm；Bottleneck (2) is welded on bottle (1) above, and external screw thread (21) is provided with outside bottleneck (2), and external screw thread (21) uses three
Angle thread form is provided with multi-thread helix, bottleneck (2) outer diameter 50mm, bottleneck (2) height 55mm；It is provided with internal thread in bottle cap (3)
(35), threaded line is provided with multi start thread line, bottle cap at triangular tooth-shaped（3）The internal thread of inside setting（35）With bottleneck（2）Outside
The external screw thread of side setting（21）It is adapted setting, bottle cap (3) is by interior external bottle cap wall（37）Constitute double-layer interlayer structure, bottle cap (3)
Outer side setting is provided with there are one vapor quick-joint valve two (32) connection bottle cap interlayer (36), bottle cap (3) another side lower part is gone out
One is connected to bottle cap interlayer (36) into vapor quick-joint valve two (31)；The holes 10mm are opened in bottle cap (3) center, bottle neck (34) passes through bottle
Lid (3) central openings simultaneously weld together with bottle cap (3), and bottle neck (34) uses 304 stainless-steel pipes of outer diameter 10mm, bottle neck
(34) an outer diameter 10mm air inlet pipe is welded on bottle neck (34) the length 45mm on bottom of bottle 5mm, bottle cap (3), bottle neck (34) side
(33), air inlet pipe (33) is led to across bottle cap (3) in bottle, and air inlet pipe (33) uses 304 stainless steel tubes；Bottle neck (34) upper end is arranged
There are silicone tube (4), silicone tube (4) other end to be provided with quick-joint valve (41)；The metallic connection parts position is all using argon arc welding
Welding.
In the present invention, the stainless steel thickness of bottle (1) and bottle cap (3) is 2.5mm.
Example IV：
In a kind of classification inoculation apparatus for microbial manure liquid fermentation provided by the invention, bottle cap (3) is internally provided with
It is close by high-temperature plastic between one layer of tempered glass (38), tempered glass (38) and bottle cap (3), bottle neck (34) and air inlet pipe (33)
Envelope, LED ultraviolet lamps are provided between tempered glass (38) and bottle cap (3).
Referring to attached drawing 5,6, in of the invention, it is equipped with stainless steel lathe dog (5) outside each quick-joint valve, is screwed on clip (5)
Setting screws screw rod (51), stainless steel lathe dog（5）To be Metal Flake, the insertion of clip movable head is screwed into screw rod when use（51）Under
Gap in, by screwing screw rod with Phillips screwdriver（51）It can allow stainless steel lathe dog（5）Annulus constantly reduce to
Compress interface.
Embodiment five：
A kind of classification inoculation apparatus production method for microbial manure liquid fermentation of the invention is as follows：
（1）Bottom of bottle is made to make：One outer bottom of bottle and one is made using the stainless steel flat bottom cover of tablet compacting or spinning
A interior bottom of bottle.
（2）Bottle wall makes：Using two sets of taper veneer reeling machine compacting welding, respectively bottle inner wall (14) and bottle outer wall
(15)。
（3）Bottleneck makes：Using the common external screw thread of cylinder (21), screw thread is triangular tooth-shaped, and multi-thread helix is fabricated to band
Externally threaded bottleneck, then process a L-type bottleneck with cylinder stainless steel.
（4）Bottle outer wall (15), bottle inner wall (14) and two kinds of bottlenecks (2) will be processed and use argon arc welding welding, composition one
It is a with outer line silk bottleneck triangular interlayer stainless steel device, there is bottle interlayer (13) in centre, successively by interior bottom of bottle and outer blank back welding
It connects, forms one with outer line silk bottleneck triangular interlayer stainless steel triangular flask.
（5）Bottle cap makes：It selects internal thread pipe cap as bottle cap inner wall, triangular tooth-shaped, multi-thread helix, then makes one
Cylinder is as bottle cap wall (37), by the stainless steel ring plate argon arc welding of pipe cap, cylinder and one fixed width with internal thread (35)
Weld together, form the bottle cap (3) with bottle cap interlayer (36), the hole of diameter 10mm is opened in bottle cap (3) center, by outer diameter
The 304 stainless steel tube bottle necks (34) of 10mm pass through bottle cap (3) argon arc welding welding, open 10mm's again on bottle neck (34) side
Eyelet welding taps into tracheae (33).
Line is drawn bottle cap (3) by bottle cap (3) punching by more optimized LED ultraviolet lamps (39) bonding in bottle cap (3)
Outside, tempered glass (38) is installed under LED ultraviolet lamps (39), high-temperature plastic is used in combination to seal.
Embodiment six：
In a kind of classification inoculation apparatus for microbial manure liquid fermentation for being applicable in the present invention, first scrubbed clean with wash bottle
Each position covers bottle cap (3), and each vapor is accessed to the quick-joint valve for picking out button and silicone tube (4) end with clip (5)
(41) it fixes, screws and screw screw rod (51) on clip (5), then by the air inlet pipe (33) on bottle cap in bottle (1)
Boiling water is injected, then by leading under vapor quick-joint valve one (11) and bottle cap into vapor quick-joint valve two (31) under bottle (1)
Enter vapor, after washing and sterilizing, compressed air is pressed by air inlet pipe (33), boiling water is excluded from bottle neck (34), reaches bottle neck
(34) effect to sterilize, by being packed into the microbial manure liquid fermentation strain to be inoculated in the inoculation bottle of high-temperature sterilization processing,
The high-temperature resistant rubber set by sterilization treatment is all blocked outside quick-joint valve, to rubber sleeve access inoculation tank is removed when needing, is passed through
Strain is transferred to the fermentation tank to be inoculated with by air inlet pipe (33) indentation sterilizing compressed air.
As described above, you can preferably realize that the present invention, the above embodiments are only the preferred implementation side to the present invention
Formula is described, and is not defined to the scope of the present invention, and under the premise of not departing from design spirit of the present invention, this field is general
The various modifications and improvement that logical technical staff makes technical scheme of the present invention, should all fall into present invention determine that protection domain
It is interior.
Claims (7)
1. a kind of classification inoculation apparatus for microbial manure liquid fermentation, including bottle, bottleneck, bottle cap and silicone tube, feature
It is, bottle uses the double-layer interlayer structure of 304 stainless steels, bottle inner wall and bottle outer wall to constitute bottle sandwich,
Bottle includes bottle wall and bottom of bottle two parts, and bottom of bottle and bottle wall are welded into circular arc Pyrex flat bottom vial, and bottle top side is provided with one
It is a go out one air expansion bottle interlayer body of vapor quick-joint valve inside, the bottle lower part other side setting there are one into vapor quick-joint valve one connect
Inside logical bottle interlayer, bottom of bottle diameter 208-290mm, bottle wall height 393-465mm；Bottleneck is welded on above bottle, outside bottleneck
It is provided with external screw thread, external screw thread is provided with multi-thread helix, bottleneck outer diameter 45-63 .5mm, bottleneck height using triangular tooth-shaped
50-60mm；It is provided with internal thread in bottle cap, threaded line is provided with multi start thread line, is arranged on the inside of bottle cap interior at triangular tooth-shaped
The external screw thread being arranged on the outside of screw thread and bottleneck is adapted setting, and bottle cap constitutes double-layer interlayer structure by interior external bottle cap wall, outside bottle cap
Side setting there are one go out vapor quick-joint valve two be connected to bottle cap interlayer, another side lower part of bottle cap be provided with one it is fast into vapor
It connects valve two and is connected to bottle cap interlayer；The holes 10mm are opened in bottle cap center, bottle neck passes through bottle cap central openings and welds together with bottle cap,
Bottle neck uses 304 stainless-steel pipes of outer diameter 10mm, bottle neck bottle neck length 40-50mm, bottle neck on bottom of bottle 3-10mm, bottle cap
An outer diameter 10mm air inlet pipe is welded on side, and air inlet pipe is led to across bottle cap in bottle, and air inlet pipe uses 304 stainless steel tubes；Bottle neck
Upper end is provided with silicone tube, and silicone tube other end is provided with quick-joint valve；It is all welded using argon arc welding the metallic connection parts position
It connects；Closure inside is provided with one layer of tempered glass, close by high-temperature plastic between tempered glass and bottle cap, bottle neck and air inlet pipe
Envelope, is provided with LED ultraviolet lamps between glass and bottle cap；Bottom of bottle is suppressed using tablet or the stainless steel flat bottom cover of spinning；Bottle wall
It is suppressed and is welded using taper veneer reeling machine.
2. being used for the classification inoculation apparatus of microbial manure liquid fermentation as described in claim 1, which is characterized in that silica gel pipe end
Quick-joint valve outside be provided with heat safe rubber sleeve.
3. being used for the classification inoculation apparatus of microbial manure liquid fermentation as described in claim 1, which is characterized in that bottle and bottle cap
Stainless steel thickness be 2mm-3mm.
4. being used for the classification inoculation apparatus of microbial manure liquid fermentation as described in claim 1, which is characterized in that bottom of bottle diameter
208mm, bottle wall height 393mm.
5. being used for the classification inoculation apparatus of microbial manure liquid fermentation as described in claim 1, which is characterized in that bottleneck outer diameter
45mm, bottleneck height 50mm.
6. being used for the classification inoculation apparatus of microbial manure liquid fermentation as described in claim 1, which is characterized in that each quick-joint valve
Outside is equipped with stainless steel lathe dog
7. a kind of production method for microbial manure liquid fermentation classification inoculation apparatus as claimed in any one of claims 1 to 6,
Specific preparation method is as follows：
（1）Bottom of bottle is made to make：It is made in an outer bottom of bottle and one of the stainless steel flat bottom cover of tablet compacting or spinning
Bottom of bottle；
（2）Bottle wall makes：Using two sets of taper veneer reeling machine compacting welding, respectively bottle inner wall and bottle outer wall；
（3）Bottleneck makes：Using the common external screw thread of cylinder, screw thread is triangular tooth-shaped, and multi-thread helix is fabricated to externally threaded
Bottleneck, then process a L-type bottleneck with cylinder stainless steel；
（4）Bottle outer wall, bottle inner wall and two kinds of bottleneck argon arc welding weldings will be processed, composition one is with outer line silk bottleneck three
There are bottle interlayer in angular interlayer stainless steel device, centre, successively connect interior bottom of bottle and outer blank back welding, form one with outer line silk
Bottleneck triangular interlayer stainless steel triangular flask；
（5）Bottle cap makes：It selects internal thread pipe cap as bottle cap inner wall, triangular tooth-shaped, multi-thread helix, then makes a cylinder
As bottle cap wall, together with the stainless steel ring plate argon arc welding welding with female pipe cap, cylinder and one fixed width, shape
At the bottle cap with bottle cap interlayer, the hole of diameter 10mm is opened in bottle cap center, the 304 stainless steel tube bottle necks of outer diameter 10mm are passed through
Bottle cap argon arc welding welding, the eyelet welding for opening a 10mm again beside bottle neck tap into tracheae.
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| CN108384708A (en) * | 2018-05-23 | 2018-08-10 | 宁夏西麒麟生物科技有限公司 | A kind of sterile controlled amounts type classification inoculation apparatus of seeding tank and the sterile controlled amounts type inoculation method for fermenting or cultivating |
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