CN203748376U - Device for culturing arabidopsis and observing growth of arabidopsis in close-to-nature state - Google Patents
Device for culturing arabidopsis and observing growth of arabidopsis in close-to-nature state Download PDFInfo
- Publication number
- CN203748376U CN203748376U CN201420068912.8U CN201420068912U CN203748376U CN 203748376 U CN203748376 U CN 203748376U CN 201420068912 U CN201420068912 U CN 201420068912U CN 203748376 U CN203748376 U CN 203748376U
- Authority
- CN
- China
- Prior art keywords
- casing
- culture tube
- observation
- arabidopsis
- cultivate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The utility model relates to a device for culturing arabidopsis and observing growth of arabidopsis in a close-to-nature state. A culture box and an observation box are connected movably; the cultivation box comprises a culture tube holder, a culture medium holder and a culture medium cover plate, the culture tube holder is arranged on the upper portion of the culture box to partition the culture box into an overground part growth area and a root system growth area, the culture tube holder is provided with at least one culture tube supporting groove, the culture medium holder is arranged in the root system growth area, the culture medium cover plate is arranged in the root system growth area, and the plate surface of the culture medium cover plate is tangential to the bottom of the at least one culture tube supporting groove during filling of culture medium; the observation box comprises vent holes, a light filtering layer and a light shielding layer, the light filtering layer is arranged on the side wall of the observation box, the light shielding layer covers the light filtering layer and is movably connected with the observation box, and the vent holes are formed in the observation box. The device enables the overground part of the arabidopsis to be irradiated and the root system to grow under specific-wave-length light or in a completely dark environment, and is free of sucrose addition and accordant with a natural growth environment of plants.
Description
Technical field
The application belongs to the cultivation technical field of model plant arabidopsis, particularly a kind of for cultivate the device of growing with observation arabidopsis under nearly nature.
Background technology
Arabidopsis has become one of the model plant in plant science field, and plant research person can not only excavate valuable genetic resources from arabidopsis, can also study with the genetic conversion system of arabidopsis the gene function of other plant.Although can cultivate arabidopsis plant with artificial dielectrics such as vermiculite or peat, but can not real-time monitored analyze root growth situation etc., therefore, plant research person cultivates arabidopsis thaliana with agar growth plate all the time, the method is simple to operate, be easy to the growth of observation of plant, many valuable Arabidopsis Mutants are all to use the method screening and identification out.Yet, under the cultivating system of this arabidopsis agar growth plate (traditional culture apparatus), the equal irradiation of the overground part of plant and root system, root system is also grown in the environment that Exogenous Sucrose adds, and this and the natural growing environment of plant are different larger.As everyone knows, under natural growing environment, the overground part irradiation of plant and root growth adds in dark environment and without Exogenous Sucrose.So, use the physiology of traditional culture apparatus research arabidopsis and genetic mechanism to bring insecure result of study to plant research person.
Summary of the invention
The technical problem solving: for above-mentioned technological deficiency, it is a kind of for cultivate the device of growing with real-time monitored arabidopsis under nearly nature that the application provides, this device can make the overground part irradiation of arabidopsis and root growth adds in dark environment and without Exogenous Sucrose, and has kept the feature of " conventional apparatus " easy operating.New equipment gives plant and approaches natural growing environment, avoids the generation of " not rigorous result " in research process, and person can draw " compared with the conclusion of reality " to make plant research.
Technical scheme: for cultivate the device of growing with real-time monitored arabidopsis under nearly nature, comprise cultivation casing, observe casing and culture tube, cultivate casing and the flexible connection of observation casing; Described cultivation casing comprises culture tube bracket, medium bracket and medium cover plate, described culture tube bracket is located at the top of cultivating casing, cultivation casing is divided into overground part vitellarium and root growth district, culture tube bracket is provided with at least one culture tube bracket, medium bracket is located in root growth district, fit with cultivating cabinet wall, medium cover plate is located in root growth district, and culture tube bracket bottom when its plate face and perfusion cultures base is tangent; Described observation casing comprises ventilation hole, filter layer and shadow shield, and filter layer is located at the sidewall of observing casing, and shadow shield is covered on filter layer, and is flexibly connected with observing casing, and ventilation hole is located at and is observed on casing.
Described medium bracket is provided with the stressing device shifting out in Jiang Qicong root growth district.
Described medium cover plate is provided with at least one through hole.
Described inspection box body sidewall is provided with the chute sliding for shadow shield.
Also comprise oxygen increasing pump, described ventilation hole is connected with oxygen increasing pump.
Culture tube is upper coarse and lower fine inverted round stage communicating structure, the upper fixture that prevents that culture tube from rotating that is provided with along the mouth of pipe, and lower tangent along the mouth of pipe and medium cover plate, described fixture is bulge-structure.
Described culture tube bracket is provided with the stopping means coordinating with culture tube fixture, and described stopping means is groove.
Described filter layer is green color filter, green color filter can only allow the green glow of visible ray enter, this be because green glow on root growth without impact, Red and blue light has impact to root growth, under green glow, observe root system, can avoid the impact of Red and blue light on root system, therefore make root system observation more accurate.
Beneficial effect: compare with conventional apparatus, the application's device can make the overground part irradiation of arabidopsis and root growth in lower or complete darkness environment and add without sucrose, meets the self-sow environment (Fig. 1) of plant at special wavelength light (green glow).The application's device can with oxygen increasing pump coupling, can according to cultivated plant growth, require to regulate in real time condition of culture, the coordinate system that incubator cover plate is provided with can facilitate experimenter to observe the growth details of root system, accurately locate the seedling on each incubator cover plate, casing is comprised of the material of two kinds of light transmissions, the equipment that need to be connected with casing all can connect by the light-proof material of lower floor, the harmful effect of having avoided interface light leak to cause plant root growth, light transmissive material part can facilitate the growth of experimenter's observation of plant root system, thereby the growing way of judgement plant, light transmissive material part is also attached with shadow shield, shadow shield has own independent chute, after experimenter observes, can reduce the required dark surrounds of root growth, reduce as much as possible the impact of light on plant.We have further compared growth and the response change of arabidopsis under these two kinds of culture apparatuses, and result of study shows: compare the application's device energy natural reality ground reflection overground part of arabidopsis and the growth (table 1) of root system with traditional device; The response of reflection root system in the application's device energy natural reality ground to gravity; The polarity of the application's device energy natural reality ground reflection tip of a root growth hormone distributes.Meanwhile, this device, after finishing using, is cleared up conveniently, and medium bracket gapless fits in cultivation cabinet wall, and medium can be poured in bracket, therefore can be by medium bracket by used medium global purge.In addition, tangent by medium cover plate and culture tube bracket bottom, and culture tube is upper coarse and lower fine communicating structure, while having guaranteed arabidopsis root growth to medium interface, can more naturally fit with medium, the usage amount problem of perfusion cultures base while also having solved each experiment, by a through hole on medium cover plate, inject medium, another through hole exhaust, has guaranteed smooth encapsulating, and it is smooth to get rid of bubble assurance media surface completely.
Accompanying drawing explanation
Fig. 1 is for cultivate the structural representation of the device of growing with observation arabidopsis under nearly nature;
Fig. 2 is culture tube structural representation;
In figure, cultivate casing 1, observe casing 2, culture tube bracket 3, culture tube bracket 4, medium bracket 5, medium cover plate 6, through hole 7, ventilation hole 8, filter layer 9, shadow shield 10, chute 11, fixture 12.
Embodiment
Below in conjunction with instantiation and accompanying drawing, further set forth the application.Should be understood that these embodiment, only for the application is described, do not form the restriction to claim scope, other alternative means that it may occur to persons skilled in the art that, all within the scope of the application's claim.
Embodiment 1:
Device for cultivation under nearly nature with the growth of observation arabidopsis, comprises and cultivates casing 1, observation casing 2 and culture tube, cultivates casing 1 and observe casing 2 to be flexibly connected; Described cultivation casing 1 comprises culture tube bracket 3, medium bracket 5 and medium cover plate 6, described culture tube bracket 3 is located at the top of cultivating casing, cultivation casing is divided into overground part vitellarium and root growth district, culture tube bracket is provided with at least one culture tube bracket 4, medium bracket 5 is located in root growth district, medium cover plate 6 is located in root growth district, and culture tube bracket 4 bottoms when its plate face and perfusion cultures base are tangent; Described observation casing 2 comprises ventilation hole 8, filter layer 9 and shadow shield 10, and filter layer 9 is located at the sidewall of observing casing 2, and shadow shield 10 is covered on filter layer, and is flexibly connected with observing casing, and ventilation hole 8 is located at and is observed on casing 2.Described medium bracket 5 is provided with the stressing device shifting out in Jiang Qicong root growth district.Described medium cover plate is provided with a pair of through hole 7, and while injecting medium in a through hole wherein, another through hole is for exhaust.Described observation casing 2 sidewalls are provided with the chute 11 sliding for shadow shield 10.Also comprise oxygen increasing pump, described ventilation hole 8 is connected with oxygen increasing pump.Culture tube is upper coarse and lower fine communicating structure, and the upper fixture 12 that prevents that culture tube from rotating that is provided with along the mouth of pipe is lower tangent along the mouth of pipe and medium cover plate.Culture tube bracket 4 is provided with the stopping means coordinating with culture tube fixture.Described fixture is bulge-structure.Described stopping means is groove.Described filter layer 9 is green color filter, green color filter can only allow the green glow of visible ray enter, this be because green glow on root growth without impact, Red and blue light has impact to root growth, under green glow, observe root system, can avoid the impact of Red and blue light on root system, therefore make root system observation more accurate.
Traditional arabidopsis device shortcoming be root system see light and system airtight, make so airborne CO
2enter not go, arabidopsis can not carry out with CO
2photosynthesis for bed material, so added the sucrose of 1%wt-3%wt on plate, required to carbohydrate to supplement plant, do not meet the self-sow state (root system see light and grow in the environment that sucrose adds, overground part can not carry out normal photosynthesis) of plant.Therefore, we have designed the new equipment of cultivating under nearly nature with the growth of observation arabidopsis.It is (12.5 * 3.5 * 12.5cm that new equipment is cultivated casing 1 size, L * W * H), the culture tube bracket of cultivating casing 1 is interior for placing culture tube, this culture tube can fill the agaropectin of dissolving, until agaropectin solidify with cooling after arabidopsis seed can be sowed on culture tube, arabidopsis seed can be grown being equipped with on the culture tube of agaropectin, its root system can pass culture tube by agaropectin, cultivate in casing 1 and also filled a little agaropectins, after it solidifies, tangent with culture tube bottom level, the arabidopsis root stretching out from culture tube so just in time also can paste the agaropectin growth of cultivating after solidifying casing 1, as shown in Figure 1, cultivate casing 1 and observe the same size of casing 2, both can synthesize an integral body, the ventilation hole of inspection box body side surface can with oxygen increasing pump coupling, observing has coordinate system and with the shadow shield of independent chute on casing, oxygen increasing pump can be constantly to oxygenation in incubator, can make like this arabidopsis root growth ambient oxygen abundance required to maintain growth, when needs are monitored arabidopsis root growth, pulling open shadow shield just can directly observe the root growth situation of arabidopsis and read extent of the root system according to the scale on coordinate system, green color filter after shadow shield can only allow green glow enter, green glow can not affect root growth, we can be when observing root system so, avoid the impact on root system of Red and blue light in visible ray, the observation of let us is more accurate.Therefore, by such design, new equipment can make arabidopsis root growth add without sucrose in dark surrounds (during observation in green glow environment), and additionally top grows in open air, can airborne CO
2for bed material carries out normal photosynthesis.
New equipment can be cultivated the application in growing with real-time monitored arabidopsis under nearly nature, concrete grammar is: what heating was melted contains 0.6% agar (w/v, g/mL) MS medium pours into culture tube and cultivates casing 1, by tangent with culture tube bracket bottom after the culture medium solidifying in medium cover plate 6 assurance devices, after cooling culture tube is put into the culture tube bracket of cultivating casing 1, then will be observed casing 2 and cultivate the synthetic integral body of casing 1, then, arabidopsis seed is sowed to culture tube surface, after emerging, the arabidopsis overground part with greenery grows in open air and (can carry out normal photosynthesis), the root system of the arabidopsis stretching out from tubule just in time also can paste the agaropectin growth of cultivating after solidifying casing 1 (in growth and dark environment and add without sucrose), during observing, filter layer can only allow green glow enter under complete natural light is irradiated, do not disturb growing of root system, facilitate experimenter to observe, simultaneously, also can use shadow shield to guarantee that its arabidopsis grows the in the situation that of complete darkness, in the time of need to observing, pull open shadow shield, monitoring arabidopsis root length, growing state with judgement arabidopsis, and open the oxygen content in oxygen increasing pump regulation culture case.After having tested, can, by medium bracket by used medium global purge, facilitate follow-up cleaning to use.
Embodiment 2:
Adopt the device of embodiment 1 to test as follows
Plant growth analysis
Plant is measured immediately after sampling, dry weight, total root length, lateral root length, main root are shown in to angle of bend and the laser co-focusing assay method based on green fluorescent protein (GFP) of principal characteristic article (the Xu and Shi that we deliver, Plant and Soil, 2007,301:17-28; Xu et al., New Phytologist, 2013,197:139-50).
Statistical analysis
With SPSS13.0Duncan ' s Multiple Range Test, carry out statistical analysis (P<0.05).
Result and discussion
The growth of root system and response
We have studied, and in conventional apparatus, (root system is shown in light and grows in sucrose and add environment, and overground part can not carry out with CO in airtight device
2photosynthesis for bed material) (environment that root growth does not add in dark and sucrose, overground part can carry out with CO in open environment with new equipment
2photosynthesis for bed material) growth and the response of arabidopsis under culture systems.First, cultivate lower arabidopsis plant compare with conventional apparatus, new equipment cultivates that lower arabidopsis main root is grown, lateral root is short, overground part and all minimizings (table 1) to some extent of weight of root system.The second, cultivate comparing to principal characteristic of lower arabidopsis main root with conventional apparatus, the arabidopsis main root of new equipment under cultivating to the response of gravity so strong.The 3rd, the distribution situation comparison of the arabidopsis tip of a root growth hormone under cultivating with conventional apparatus, the arabidopsis tip of a root growth hormone under new equipment cultivation has been shown polarity distribution.Therefore, the above results shows that new equipment can give plant and approach natural growing environment, avoids the generation of " not rigorous result " in research process, and person can draw " compared with the conclusion of reality " to make plant research.
The growing state of wild type arabidopsis (Ws) under two kinds of devices of table 1
Claims (10)
1. the device of growing for cultivation under nearly nature and observation arabidopsis, is characterized in that: comprise and cultivate casing (1), observation casing (2) and culture tube, cultivate casing (1) and observe casing (2) flexible connection; Described cultivation casing (1) comprises culture tube bracket (3), medium bracket (5) and medium cover plate (6), described culture tube bracket (3) is located at the top of cultivating casing, cultivation casing is divided into overground part vitellarium and root growth district, culture tube bracket is provided with at least one culture tube bracket (4), medium bracket (5) is located in root growth district, with the laminating of cultivation cabinet wall, medium cover plate (6) is located in root growth district, and culture tube bracket (4) bottom when its plate face and perfusion cultures base is tangent; Described observation casing (2) comprises ventilation hole (8), filter layer (9) and shadow shield (10), filter layer (9) is located at the sidewall of observing casing (2), shadow shield (10) is covered on filter layer, and is flexibly connected with observing casing, and ventilation hole (8) is located at and is observed on casing (2).
2. according to claim 1 for cultivate the device of growing with observation arabidopsis under nearly nature, it is characterized in that described medium bracket (5) is provided with the stressing device shifting out in Jiang Qicong root growth district.
3. according to claim 1 for cultivate the device of growing with observation arabidopsis under nearly nature, it is characterized in that described medium cover plate is provided with at least one through hole (7).
4. according to claim 1 for cultivate the device of growing with observation arabidopsis under nearly nature, it is characterized in that described observation casing (2) sidewall is provided with the chute (11) sliding for shadow shield (10).
5. according to claim 1 for cultivate the device of growing with observation arabidopsis under nearly nature, characterized by further comprising oxygen increasing pump, described ventilation hole (8) is connected with oxygen increasing pump.
6. according to claim 1 for cultivate the device of growing with observation arabidopsis under nearly nature, it is characterized in that described culture tube is upper coarse and lower fine inverted round stage communicating structure, the upper fixture (12) that prevents that culture tube from rotating that is provided with along the mouth of pipe, lower tangent along the mouth of pipe and medium cover plate.
7. according to claim 6 for cultivate the device of growing with observation arabidopsis under nearly nature, it is characterized in that described culture tube bracket (4) is provided with the stopping means coordinating with culture tube fixture.
8. according to claim 6 for cultivate the device of growing with observation arabidopsis under nearly nature, it is characterized in that described fixture is bulge-structure.
9. according to claim 7 for cultivate the device of growing with observation arabidopsis under nearly nature, it is characterized in that described stopping means is groove.
10. according to claim 1 for cultivate the device of growing with observation arabidopsis under nearly nature, it is characterized in that described filter layer (9) is green color filter.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420068912.8U CN203748376U (en) | 2014-02-18 | 2014-02-18 | Device for culturing arabidopsis and observing growth of arabidopsis in close-to-nature state |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420068912.8U CN203748376U (en) | 2014-02-18 | 2014-02-18 | Device for culturing arabidopsis and observing growth of arabidopsis in close-to-nature state |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203748376U true CN203748376U (en) | 2014-08-06 |
Family
ID=51243682
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420068912.8U Expired - Fee Related CN203748376U (en) | 2014-02-18 | 2014-02-18 | Device for culturing arabidopsis and observing growth of arabidopsis in close-to-nature state |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203748376U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103828701B (en) * | 2014-02-18 | 2016-04-20 | 中国科学院南京土壤研究所 | For cultivating the device grown with observation arabidopsis under near-nature forest state |
| CN109463355A (en) * | 2018-09-28 | 2019-03-15 | 陈雅丝 | A kind of observation control method for the insect life observation case being equipped with baby spot |
| CN116762701A (en) * | 2023-07-31 | 2023-09-19 | 扬州大学 | Device and method for detecting water-based nature of plant roots in near-natural state |
-
2014
- 2014-02-18 CN CN201420068912.8U patent/CN203748376U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103828701B (en) * | 2014-02-18 | 2016-04-20 | 中国科学院南京土壤研究所 | For cultivating the device grown with observation arabidopsis under near-nature forest state |
| CN109463355A (en) * | 2018-09-28 | 2019-03-15 | 陈雅丝 | A kind of observation control method for the insect life observation case being equipped with baby spot |
| CN116762701A (en) * | 2023-07-31 | 2023-09-19 | 扬州大学 | Device and method for detecting water-based nature of plant roots in near-natural state |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN204810887U (en) | Percentage of seedgermination detects incubator | |
| WO2017161698A1 (en) | Plant hydroponics rack and plant-growth protection system | |
| CN102763573A (en) | Plant root system in-situ dynamic observation device, system and method under soil culture conditions | |
| CN205962176U (en) | Experimental hydroponic device of soybean nutritional deficiency | |
| CN206517933U (en) | Hydroponic system for sugarcane root system research | |
| CN209234453U (en) | A kind of multi-functional seed rearing device of use for laboratory | |
| CN203748376U (en) | Device for culturing arabidopsis and observing growth of arabidopsis in close-to-nature state | |
| CN103828701B (en) | For cultivating the device grown with observation arabidopsis under near-nature forest state | |
| CN103197050A (en) | In-situ measurement device for measuring plant root respiration | |
| CN208657483U (en) | A kind of novel growth of watermelon greenhouse stereo cultivation frame | |
| CN205946812U (en) | Plant culture dish | |
| CN103026892A (en) | Cultivating device of antrodia camphorata | |
| CN200987313Y (en) | Plant root system development cultivation observing device | |
| CN205408974U (en) | Plant roots viewing device that grows | |
| CN206760344U (en) | A kind of multi-functional plant cultivates growth cabinet | |
| CN201733690U (en) | Device for in situ screening and cultivation of transgenosis arabidopsis thaliana sprouts | |
| CN205756178U (en) | A kind of high flux screening allelopathic Rice Germplasm Resources device | |
| CN208908784U (en) | A kind of corn seed culture apparatus | |
| CN205861658U (en) | The allelopathic test device of secretions from plant roots | |
| CN207040584U (en) | A kind of flower and fish co-culturing device | |
| CN206043034U (en) | A kind of plant individual plant seedling water planting transplantation device | |
| CN109089656A (en) | A kind of monolithic processor controlled plant culture box | |
| CN115735488A (en) | Medicinal plant ecological landscape zone planning method for protecting ecological environment of yellow river basin | |
| CN108684519A (en) | For cultivating and detecting the hydrotropic device and method of arabidopsis root | |
| CN209727785U (en) | Individual plants root system observation device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140806 Termination date: 20150218 |
|
| EXPY | Termination of patent right or utility model |