CN203700344U - Stair type reaction lactose cholate fermentation tube for measuring coliform in sanitary inspection - Google Patents
Stair type reaction lactose cholate fermentation tube for measuring coliform in sanitary inspection Download PDFInfo
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- CN203700344U CN203700344U CN201420072163.6U CN201420072163U CN203700344U CN 203700344 U CN203700344 U CN 203700344U CN 201420072163 U CN201420072163 U CN 201420072163U CN 203700344 U CN203700344 U CN 203700344U
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Abstract
The utility model discloses a stair type reaction lactose cholate fermentation tube for measuring coliform in sanitary inspection and belongs to the technical field of medical and laboratory equipment. The stair type reaction lactose cholate fermentation tube is made of quartz glass and comprises large fermentation tubes (9), small fermentation tubes (13) and an auxiliary structure, wherein the large fermentation tubes are used for culturing the coliform; the auxiliary structure is designed for realizing the stair type reaction; three large fermentation tubes and three small fermentation tubes are included in each group; one Durham's small inverted tube (10) is arranged in each fermentation tube; the large fermentation tubes are connected with each other through joints (3) between tubes, and the joints are made of quartz glass, so are the small fermentation tubes; the base parts of every two large fermentation tubes are communicated with each other through communicating tubes (8) or liquid inlet communicating tubes (11), and so are the base parts of every two small fermentation tubes; a balance hole (15) is formed in the upper end of the last large fermentation tube and the upper end of the last small fermentation tube. The stair type reaction lactose cholate fermentation tube is simple to manufacture, convenient to operate and high in practicality.
Description
Technical field
The present invention relates to coliform mensuration in a kind of sanitary inspection and use graduated response bile salt lactose fermentation tube, belong to medical treatment and laboratory equipment technical field.
Background technology
Coliform means a group energy ferment lactose, produces sour aerogenesis, aerobic and amphimicrobian Gram-negative bactacin.This bacterium mainly comes in human and animal excreta, therefore evaluate the hygienic quality of food using this as fecal pollution index, infers the possibility of whether polluting pathogen enterobacteria in food.In food, coliform number system represents with the maximum most probable number (MPN) of coliform in 100mLg sample (most probable number, MPN).When coliform is detected, known experimental implementation step is: (1) review sample dilution: 1. with aseptic technique, sample 25mL or g are put in the sterilizing vial of 225mL sterile saline or other diluents and give in the granulated glass sphere or sterilizing mortar of putting proper amt in bottle, make the even diluent of 1: 10 through shake well or grinding.The most handy homogenizer of solid sample, with the velocity process 1min of 8000-10000r/min, makes the even diluent of 1: 10.2. draw 1: 10 diluent 1mL with 1mL sterilizing suction pipe, inject and contain 9mL sterile saline or other diluents in vitro, jolting test tube mixes, and makes the diluent of 1: 100.3. separately get 1mL sterilizing suction pipe, take turns doing 10 times by upper operation and increase progressively diluent, often increase progressively dilution once, use 1 1mL sterilizing suction pipe instead.4. according to food hygienic standard requirement or the estimation to sample pollution condition, select three extent of dilution, each extent of dilution, inoculation 3 pipes.(2) lactose-fermentation test: sample to be checked is inoculated in bile salt lactose fermentation tube, and inoculum size, the above person of 1mL, is used extra quality bile salt lactose fermentation tube, and inoculum size, 1mL and person below 1mL, is used pure bile salt lactose fermentation tube.Each extent of dilution inoculation 3 pipes, put in 36 ± 1 ℃ of incubators, cultivate 24 ± 2h, as aerogenesis not of all lactose cholate fermentation tube, can be reported as coliform feminine gender, if any aerogenesis or variable color person, are undertaken by follow procedure.(3) separation and Culture: the fermentation tube difference transferred species of aerogenesis, on eosin methylene blue agar flat board, is put in 36 ± 1 ℃ of incubators, cultivate 18-24h, then take out, observe colonial morphology, and do gramstaining and confirmatory test.(4) confirmatory test: on above-mentioned flat board, the suspicious coliform bacterium colony of picking 1-2 is carried out gramstaining, inoculates lactose fermentation tube simultaneously, puts in 36 ± 1 ℃ of incubators and cultivates 24 ± 2h, observes aerogenesis situation.All lactose pipe aerogenesis, the negative bactacin of gramstaining, can be reported as coliform-positive.(5) report: according to the pipe number that turns out to be coliform-positive, look into MPN key, report the MPN value of every 100mLg coliform.In known experimental implementation process, because lactose fermentation tube quantity used is more, comparatively trouble operates, simultaneously, lactose fermentation tube used is due to multi-pass operations, contaminated chance is very many, thereby must authority's sanitary inspection requirement accurately bring very large challenge for result, is also the difficult problem of pendulum in face of sanitary inspection personnel.How to design a kind of step that can reduce experimental implementation, allow sanitary inspection personnel number of operations reduce, so multiple bile salt lactose fermentation tubes can be communicated with thereby as much as possible reduce the contaminated difficult problem invention one of being badly in need of solving that becomes of sample, it is necessary that single job can complete coliform mensuration graduated response bile salt lactose fermentation tube in the sanitary inspection that has or not coliform checking in sample.
Summary of the invention
In order to solve the step that reduces experimental implementation, allow sanitary inspection personnel number of operations reduce, thereby as much as possible reduce the contaminated difficult problem of sample, the invention provides coliform mensuration graduated response bile salt lactose fermentation tube in a kind of sanitary inspection, in this sanitary inspection, coliform mensuration is communicated with multiple bile salt lactose fermentation tubes with graduated response bile salt lactose fermentation tube, single job can complete in sample and have or not coliform to verify, reach the step that reduces experimental implementation, allow sanitary inspection personnel number of operations reduce, thereby as much as possible reduce the contaminated object of sample.
The technical solution adopted for the present invention to solve the technical problems is:
In sanitary inspection, coliform is measured and is used graduated response bile salt lactose fermentation tube, and material is silica glass, comprises and cultivates the large fermentation tube 9, little fermentation tube 13 of coliform and formed by realizing the supplementary structure that graduated response designs; The interior diameter of described large fermentation tube 9 is 2-5 centimetre, wall thickness 1-3 millimeter, and length is 3-10 centimetre, totally 3, between the pipe by silica glass matter, connect 3 each other and link together, locate in one plane or be equilateral triangle to be close together mutually each other; The base portion of 3 large fermentation tubes 9 was communicated with between two by communicating pipe 8, in the upper end that comes last large fermentation tube 9, was provided with equalizing port 15; The interior diameter of described little fermentation tube 13 is 2-5 centimetre, wall thickness 1-3 millimeter, length is 1-5 centimetre, upper and lower 2 groups, every group 3, totally 6,3 every group little fermentation tubes 13 connect 3 between the pipe by silica glass matter each other and link together, and locate in one plane or be equilateral triangle to be close together mutually each other; The base portion of 3 little fermentation tubes 13 was communicated with between two by feed liquor communicating pipe 11, in the upper end that comes last little fermentation tube 13, was also provided with equalizing port 15, was provided with 1 little voltage regulator tube 10 of Du Shi in each large fermentation tube 9 and little fermentation tube 13; Described for realize supplementary structure that graduated response designs comprise between liquid injection tube lid 1, upper slow injection capsule 2, pipe, connect 3, diluted liquid tube 4, first liquid pipe 5, liquid injection tube 6, lower slow injection capsule 7, communicating pipe 8, the little voltage regulator tube 10 of Du Shi, feed liquor communicating pipe 11, separating tube 12, three fluid-through tubes 14, equalizing port 15 and catheter 16.
Described liquid injection tube lid 1 silica glass quality, point upper and lower two portions, upper part is cylindrical, and thickness is 0.5-2 centimetre, and diameter is 3-8 centimetre; Lower part truncated cone-shaped, upper base is free, and diameter is 1.5-4 centimetre; Go to the bottom and be connected to upper part lower surface central authorities, diameter is 2-5 centimetre, is highly 1-5 centimetre, side frosted.
Described upper slow injection capsule 2 spherical shape, diameter is 1.6-4.6 centimetre, and lower end is communicated with just liquid pipe 5 upper end open, and upper end open is in liquid injection tube 6 upper pipes; Lower slow injection capsule 7 spherical shape, diameter is 1.6-4.6 centimetre, and lower end is communicated with communicating pipe 8 upper end open, and upper end is communicated with first liquid pipe 5 lower ending openings.
Described diluted liquid tube 4 is that the central authorities that are positioned at first liquid pipe 5 and catheter 16 are communicated with the circular pipe of three fluid-through tubes 14 to little fermentation tube 13, and internal diameter is 2-5 millimeter, wall thickness 0.2-1 millimeter; Just liquid pipe 5 internal diameters are 5-10 millimeter, wall thickness 0.2-1 millimeter; Liquid injection tube 6 and catheter 16 are all pipes for protection, and interior diameter is 2-5 centimetre, wall thickness 3-5 millimeter, and length is 10-20 centimetre.
The little voltage regulator tube 10 silica glass matter of described Du Shi, internal diameter is 3-5 millimeter, wall thickness 1-3 millimeter, length is 2-3 centimetre; Feed liquor communicating pipe 11 is pipelines that little fermentation tube 13 base portions are communicated with each other, and pipe interior diameter is 2-5 millimeter, wall thickness 1-3 millimeter; Separating tube 12 is branches for three fluid-through tube 14 extensions, one group little fermentation tube 13 in the middle of lower end connects, and internal diameter is 2-5 millimeter, wall thickness 0.2-1 millimeter.
The invention has the beneficial effects as follows, in sanitary inspection, coliform mensuration is communicated with multiple bile salt lactose fermentation tubes with graduated response bile salt lactose fermentation tube, single job can complete in sample and have or not coliform to verify, reach the step that reduces experimental implementation, allow sanitary inspection personnel number of operations reduce, thereby as much as possible reduce the contaminated object of sample.In sanitary inspection, coliform is measured with the making of graduated response bile salt lactose fermentation tube simple, easy to operate, practical.
Accompanying drawing explanation
Below in conjunction with accompanying drawing, the present invention is further described.
Fig. 1 is that in sanitary inspection of the present invention, coliform is measured the one-piece construction schematic diagram with graduated response bile salt lactose fermentation tube.
Fig. 2 is that in sanitary inspection of the present invention, coliform is measured the vertical section structure schematic diagram with graduated response bile salt lactose fermentation tube.
In figure, 1. liquid injection tube lids, 2. go up slow injection capsule, 3. between pipe, connect, 4. diluted liquid tube, 5. first liquid pipe, 6. liquid injection tube, 7. descend slow injection capsule, 8. communicating pipe, 9. large fermentation tube, 10. the little voltage regulator tube of Du Shi, 11. feed liquor communicating pipe, 12. separating tubes, 13. little fermentation tubes, 14. 3 fluid-through tubes, 15. equalizing ports, 16. catheters.
Embodiment
Embodiment mono-
In sanitary inspection, coliform is measured and is used graduated response bile salt lactose fermentation tube, and material is silica glass, comprises and cultivates the large fermentation tube 9, little fermentation tube 13 of coliform and formed by realizing the supplementary structure that graduated response designs.
The interior diameter of described large fermentation tube 9 is 2-5 centimetre, wall thickness 1-3 millimeter, and length is 3-10 centimetre, totally 3, between the pipe by silica glass matter, connect 3 each other and link together, locate in one plane or be equilateral triangle to be close together mutually each other.The base portion of 3 large fermentation tubes 9 was communicated with between two by communicating pipe 8, and liquid substratum can be entered in another pipe by a pipe, and according to U-shaped pipe principle, the amount of 3 large fermentation tube 9 interior substratum is identical.Because atmospheric existence, when toward large fermentation tube 9 filling liquid substratum, or when large fermentation tube 9 is cleaned, liquid is not easy to trickle inward.In order to make liquid be full of smoothly large fermentation tube 9 or convenient cleaning, in the upper end that comes last large fermentation tube 9, be provided with equalizing port 15, the pressure at large fermentation tube 9 two ends equated, so that the high temperature and high pressure gas of liquid or sterilizing and virus killing use enters in large fermentation tube 9 smoothly from liquid injection tube 6.
The interior diameter of described little fermentation tube 13 is 2-5 centimetre, wall thickness 1-3 millimeter, length is 1-5 centimetre, upper and lower 2 groups, every group 3, totally 6,3 every group little fermentation tubes 13 connect 3 between the pipe by silica glass matter each other and link together, and locate in one plane or be equilateral triangle to be close together mutually each other.The base portion of 3 little fermentation tubes 13 was communicated with between two by feed liquor communicating pipe 11, and liquid substratum can be entered in another pipe by a pipe, and according to U-shaped pipe principle, the amount of 3 little fermentation tube 13 interior substratum is identical.In order to make liquid be full of smoothly little fermentation tube 13 or convenient cleaning, in the upper end that comes last little fermentation tube 13, also be provided with equalizing port 15, the pressure at little fermentation tube 13 two ends is equated, so that the high temperature and high pressure gas of liquid or sterilizing and virus killing use enters in little fermentation tube 13 smoothly from diluted liquid tube 4, three fluid-through tubes 14 and separating tube 12.In each large fermentation tube 9 and little fermentation tube 13, be provided with 1 little voltage regulator tube 10 of Du Shi.
Described for realize supplementary structure that graduated response designs comprise between liquid injection tube lid 1, upper slow injection capsule 2, pipe, connect 3, diluted liquid tube 4, first liquid pipe 5, liquid injection tube 6, lower slow injection capsule 7, communicating pipe 8, the little voltage regulator tube 10 of Du Shi, feed liquor communicating pipe 11, separating tube 12, three fluid-through tubes 14, equalizing port 15 and catheter 16.Liquid injection tube lid 1 silica glass quality, shape and structure, with known glass cap, are divided upper and lower two portions, and upper part is cylindrical, and thickness is 0.5-2 centimetre, and diameter is 3-8 centimetre; Lower part truncated cone-shaped, upper base is free, and diameter is 1.5-4 centimetre; Go to the bottom and be connected to upper part lower surface central authorities, diameter is 2-5 centimetre, is highly 1-5 centimetre, and side frosted, in order to increase the sealing effectiveness between liquid injection tube lid 1 and liquid injection tube 6 upper end open, reduces contaminated risk in coliform culturing process.Upper slow injection capsule 2 spherical shape, diameter is 1.6-4.6 centimetre, and lower end is communicated with just liquid pipe 5 upper end open, and upper end open is in liquid injection tube 6 upper pipes.Lower slow injection capsule 7 spherical shape, diameter is 1.6-4.6 centimetre, and lower end is communicated with communicating pipe 8 upper end open, and upper end is communicated with first liquid pipe 5 lower ending openings.The effect of upper slow injection capsule 2 and lower slow injection capsule 7 is all to allow the high-temperature gas that liquid substratum or sterilizing are used more effectively enter in large fermentation tube 9 and little fermentation tube 13.Between pipe, connect 3 silica glass quality, the pipeline that needs can be connected couples together on outer wall, makes adjacent each pipeline form an entirety.Diluted liquid tube 4 is to be positioned at the circular pipe that the first central authorities of liquid pipe 5 and catheter 16 are being communicated with three fluid-through tubes 14, and internal diameter is 2-5 millimeter, wall thickness 0.2-1 millimeter, can by diluent or just liquid import in little fermentation tube 13.Just liquid pipe 5 is the pipelines that first liquid culture medium liquid injected to large fermentation tube 9, and internal diameter is 5-10 millimeter, wall thickness 0.2-1 millimeter.The little voltage regulator tube 10 silica glass matter of Du Shi, internal diameter is 3-5 millimeter, wall thickness 1-3 millimeter, length is 2-3 centimetre, its effect is to show the colibacillus in the item inspecting of ehec infection with aerogenesis.Because bile salt lactose fermentation tube is a kind of microbiological culture media, for the cultivation of clinical, medicine, food colibacillus, in actual production, be commonly used to item inspecting and whether infect coliform, bile salt lactose fermentation tube is made acid base indicator with purpurum bromocresolis pH5.2~6.8, when production by biological acid, color becomes yellow from bluish voilet, and when microorganism aerogenesis, Du Shi is little 10 vacates one section above.Feed liquor communicating pipe 11 is pipelines that little fermentation tube 13 base portions are communicated with each other, and pipe interior diameter is 2-5 millimeter, wall thickness 1-3 millimeter.Separating tube 12 is branches for three fluid-through tube 14 extensions, liquid culture medium can be divided in the little fermentation tube 13 in the middle of installing to, and internal diameter is 2-5 millimeter, wall thickness 0.2-1 millimeter.Equalizing port 15 is arranged on the upper end that comes last large fermentation tube 9 or little fermentation tube 13, while opening, can make the pressure at large fermentation tube 9 or little fermentation tube 13 two ends equate, so that the high temperature and high pressure gas of liquid or sterilizing and virus killing use enters smoothly in large fermentation tube 9 or enters little fermentation tube 13 from diluted liquid tube 4, three fluid-through tubes 14 and separating tube 12 from liquid injection tube 6.Once pour into completely, available lid or rubber case be equalizing port 15 shutoff, in case coliform is measured and used graduated response bile salt lactose fermentation tube in extraneous bacterium or cell entry sanitary inspection.Liquid injection tube 6 and catheter 16 are all pipes for protection, and interior diameter is 2-5 centimetre, wall thickness 3-5 millimeter, and length is 10-20 centimetre.
In the time that coliform is tested, coliform mensuration in used medium and sanitary inspection is carried out to autoclaving with graduated response bile salt lactose fermentation tube, then under aseptic condition, the sample of different extension rates is injected to different fermentation tubes with syringe, in shutoff, in sanitary inspection, coliform is measured with all openings of graduated response bile salt lactose fermentation tube, can be placed in 36 ± 1 ℃ of incubators, cultivate 24 ± 2h, as aerogenesis not of all lactose cholate fermentation tube, can be reported as coliform feminine gender, if any aerogenesis person, undertaken by follow procedure: first, separation and Culture: the fermentation tube of aerogenesis is extracted to nutrient solution by equalizing port 15 with syringe under aseptic condition, transferred species is on eosin methylene blue agar flat board respectively, put in 36 ± 1 ℃ of incubators, cultivate 18-24h, then take out, observe colonial morphology, and do gramstaining and confirmatory test.Then carry out confirmatory test: on above-mentioned flat board, the suspicious coliform bacterium colony of picking 1-2 is carried out gramstaining, inoculates lactose fermentation tube simultaneously, puts in 36 ± 1 ℃ of incubators and cultivates 24 ± 2h, observes aerogenesis situation.All lactose pipe aerogenesis, the negative bactacin of gramstaining, can be reported as coliform-positive.Final report (FR): according to the pipe number that turns out to be coliform-positive, look into the maximum most probable number (MPN) of MPN, most probable number) key, report the MPN value of every 100mLg coliform.
More than show and described ultimate principle of the present invention and principal character and advantage of the present invention.The technician of the industry should be appreciated that; the present invention is not restricted to the described embodiments; that in above-described embodiment and specification sheets, describes just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications; these changes and improvements all fall in the claimed scope of the invention, and the claimed scope of the present invention plays equivalent by appending claims and defines.
Claims (5)
1. in sanitary inspection, coliform is measured and is used graduated response bile salt lactose fermentation tube, material is silica glass, comprises and cultivates the large fermentation tube (9) of coliform, little fermentation tube (13) and formed by realizing the supplementary structure that graduated response designs;
The interior diameter of described large fermentation tube (9) is 2-5 centimetre, wall thickness 1-3 millimeter, length is 3-10 centimetre, totally 3, link together by connecting (3) between the pipe of silica glass matter each other, locate in one plane or be equilateral triangle to be close together mutually each other; The base portion of 3 large fermentation tubes (9) was communicated with between two by communicating pipe (8), in the upper end that comes last large fermentation tube (9), was provided with equalizing port (15);
The interior diameter of described little fermentation tube (13) is 2-5 centimetre, wall thickness 1-3 millimeter, length is 1-5 centimetre, upper and lower 2 groups, every group 3, totally 6,3 every group little fermentation tubes (13) link together by connecting (3) between the pipe of silica glass matter each other, locate in one plane or be equilateral triangle to be close together mutually each other; The base portion of 3 little fermentation tubes (13) was communicated with between two by feed liquor communicating pipe (11), in the upper end that comes last little fermentation tube (13), also be provided with equalizing port (15), in each large fermentation tube (9) and little fermentation tube (13), be provided with 1 little voltage regulator tube of Du Shi (10);
Described comprise and between liquid injection tube lid (1), upper slow injection capsule (2), pipe, connect (3), diluted liquid tube (4), first liquid pipe (5), liquid injection tube (6), lower slow injection capsule (7), communicating pipe (8), the little voltage regulator tube of Du Shi (10), feed liquor communicating pipe (11), separating tube (12), three fluid-through tubes (14), equalizing port (15) and catheter (16) for realizing supplementary structure that graduated response designs.
2. in sanitary inspection according to claim 1, coliform is measured and is used graduated response bile salt lactose fermentation tube, it is characterized in that liquid injection tube lid (1) silica glass quality, point upper and lower two portions, upper part is cylindrical, thickness is 0.5-2 centimetre, and diameter is 3-8 centimetre; Lower part truncated cone-shaped, upper base is free, and diameter is 1.5-4 centimetre; Go to the bottom and be connected to upper part lower surface central authorities, diameter is 2-5 centimetre, is highly 1-5 centimetre, side frosted.
3. in sanitary inspection according to claim 1, coliform is measured and is used graduated response bile salt lactose fermentation tube, it is characterized in that slow injection capsule (2) spherical shape, diameter is 1.6-4.6 centimetre, lower end is communicated with just liquid pipe (5) upper end open, and upper end open is in liquid injection tube (6) upper pipes; Lower slow injection capsule (7) spherical shape, diameter is 1.6-4.6 centimetre, and lower end is communicated with communicating pipe (8) upper end open, and upper end is communicated with first liquid pipe (5) lower ending opening.
4. in sanitary inspection according to claim 1, coliform is measured and is used graduated response bile salt lactose fermentation tube, it is characterized in that diluted liquid tube (4) is that the central authorities that are positioned at first liquid pipe (5) and catheter (16) are communicated with the circular pipe of three fluid-through tubes (14) to little fermentation tube (13), internal diameter is 2-5 millimeter, wall thickness 0.2-1 millimeter; Just liquid pipe (5) internal diameter is 5-10 millimeter, wall thickness 0.2-1 millimeter; Liquid injection tube (6) and catheter (16) they are all pipes for protection, and interior diameter is 2-5 centimetre, wall thickness 3-5 millimeter, and length is 10-20 centimetre.
5. in sanitary inspection according to claim 1, coliform is measured and is used graduated response bile salt lactose fermentation tube, it is characterized in that the little voltage regulator tube of Du Shi (10) silica glass matter, and internal diameter is 3-5 millimeter, wall thickness 1-3 millimeter, and length is 2-3 centimetre; Feed liquor communicating pipe (11) is the pipeline that little fermentation tube (13) base portion is communicated with each other, and pipe interior diameter is 2-5 millimeter, wall thickness 1-3 millimeter; Separating tube (12) is a branch of three fluid-through tubes (14) extension, one group of little fermentation tube (13) in the middle of lower end connects, and internal diameter is 2-5 millimeter, wall thickness 0.2-1 millimeter.
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN111961587A (en) * | 2020-08-20 | 2020-11-20 | 江苏康正生物科技有限公司 | Water quality microorganism detection device and detection method thereof |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN111961587A (en) * | 2020-08-20 | 2020-11-20 | 江苏康正生物科技有限公司 | Water quality microorganism detection device and detection method thereof |
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