CN203212575U - Sterile culture detection device - Google Patents
Sterile culture detection device Download PDFInfo
- Publication number
- CN203212575U CN203212575U CN 201320226403 CN201320226403U CN203212575U CN 203212575 U CN203212575 U CN 203212575U CN 201320226403 CN201320226403 CN 201320226403 CN 201320226403 U CN201320226403 U CN 201320226403U CN 203212575 U CN203212575 U CN 203212575U
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- CN
- China
- Prior art keywords
- air
- hose
- sterile culture
- sampling
- pyrosphere
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn - After Issue
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The utility model relates to a sterile culture detection device which comprises a gas inlet hose, an exhaust hose, a sealed bottle and a sampling fire ring. The sampling fire ring is sleeved on an air branch pipeline, the gas inlet hose and the exhaust hose are connected into the sealed bottle, and detection culture media are filled in the sealed bottle. The sterile culture detection device has the advantages that a sterile condition of an air system can be timely found out and timely judged, and accordingly production loss is reduced.
Description
Technical field
The utility model relates to the sterile culture proofing unit in a kind of microbial cultivation process.
Background technology
Most industrial fermentations all adopt purebred cultivation, require fermenting process that the production bacterium can only be arranged, and have not allowed " assorted bacterium " to pollute.Cause the reason of fermentation microbiological contamination a lot, seed-borne fungi, air carry disease germs, equipment seepage, sterilization not thoroughly, misoperation or mismanagement be the common reason that causes the fermentation microbiological contamination, wherein to carry disease germs be the main reason that causes microbiological contamination to air.In case it is the air system microbiological contamination can cause a large amount of continuous production batch microbiological contaminations, serious to producing influence.Therefore in production process, the strict control microbiological contamination, find that in time the microbiological contamination reason has very great help to whole production.
Summary of the invention
The purpose of this utility model is to provide a kind of filtrated air sterile culture proofing unit, can in time find whether microbiological contamination of air system.
Technical solution of the present utility model is: a kind of sterile culture proofing unit, comprise air induction hose, exhaust hose, air-tight bottle and be socketed on the sampling pyrosphere of air bypass line, air induction hose and exhaust hose are connected in the air-tight bottle, and the detection substratum is housed in the air-tight bottle.
Further, air induction hose is deep at the bottom of the air-tight bottle bottle.
Further, exhaust hose position in bottle is not less than air induction hose.
Further, jagged on the sampling pyrosphere.
Again further, fluted on the sampling pyrosphere.
After adopting above technical scheme, the beneficial effect that the utility model reaches is:
By this aseptic proofing unit, can in time find the aseptic situation of air system, accomplish timely judgement, reduce the loss of producing.
Description of drawings
Fig. 1 is integral construction figure of the present utility model.
Shown in the figure: fermentor tank air branch road valve 1, air bypass line 2, sampling pyrosphere 3, handle 4, air induction hose strainer 5, air induction hose 6, air-tight bottle 7 detects substratum 8, exhaust hose 9, exhaust hose strainer 10.
Embodiment
As shown in Figure 1, a kind of sterile culture proofing unit comprises fermentor tank air branch road valve 1, air bypass line 2, and sampling pyrosphere 3, handle 4, air induction hose strainer 5, air induction hose 6, air-tight bottle 7 detects substratum 8, exhaust hose 9, exhaust hose strainer 10.
The air bypass line 2 that air induction hose strainer 5 will be connected with fermentor tank air branch road valve 1 is connected on the air induction hose 6, air induction hose 6 connects the air-tight bottle 7 that detection substratum 8 is housed, air-tight bottle 7 also is connected with exhaust hose 9, and exhaust hose strainer 10 is housed on the vapor pipe 9.
Sampling pyrosphere 3 is the semicircle steel pipes that have groove, and ethanol is housed in the groove.The handle 4 of sampling on the pyrosphere 3 of holding with a firm grip, light ethanol, be socketed on the air bypass line 2 by sampling pyrosphere 3 breach, open the air branch road valve 1 of fermentor tank, sampling pyrosphere 3 is scorching hot air bypass line 2 about 5 minutes back and forth up and down, and air bypass line 2 is discharged filtrated airs; Take out air induction hose strainer 5 then, be put into the interior sterilization of container of sterilization; Rapidly air induction hose 6 is connected on the air bypass line 2 by air induction hose strainer 5; After air after the filtration is delivered to air-tight bottle 7, detect via detecting substratum 8, cultivation is after 24 hours down at 37 ℃ for substratum 8 in the air-tight bottle 7, and sampling microscopic examination microbiological contamination situation is discharged detected gas outside the bottle finally by exhaust hose 9 and exhaust hose strainer 10.After the fermentation ventilation finishes, re-use sampling pyrosphere 3, light the ethanol on the sampling pyrosphere 3, aseptic technique disconnects air induction hose 6 fast.
Claims (5)
1. sterile culture proofing unit is characterized in that: comprise air induction hose, exhaust hose, air-tight bottle and be socketed on the sampling pyrosphere of air bypass line, air induction hose and exhaust hose are connected in the air-tight bottle, and the detection substratum is housed in the air-tight bottle.
2. a kind of sterile culture proofing unit according to claim 1, it is characterized in that: described exhaust hose position in bottle is not less than air induction hose.
3. a kind of sterile culture proofing unit according to claim 1 and 2, it is characterized in that: described air induction hose is deep at the bottom of the air-tight bottle bottle.
4. a kind of sterile culture proofing unit according to claim 1 is characterized in that: have breach on the described sampling pyrosphere.
5. a kind of sterile culture proofing unit according to claim 1, it is characterized in that: described sampling pyrosphere is provided with groove.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320226403 CN203212575U (en) | 2013-04-28 | 2013-04-28 | Sterile culture detection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320226403 CN203212575U (en) | 2013-04-28 | 2013-04-28 | Sterile culture detection device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203212575U true CN203212575U (en) | 2013-09-25 |
Family
ID=49202126
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201320226403 Withdrawn - After Issue CN203212575U (en) | 2013-04-28 | 2013-04-28 | Sterile culture detection device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203212575U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103205359A (en) * | 2013-04-28 | 2013-07-17 | 博瑞生物医药泰兴市有限公司 | Sterile culture detection device |
-
2013
- 2013-04-28 CN CN 201320226403 patent/CN203212575U/en not_active Withdrawn - After Issue
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103205359A (en) * | 2013-04-28 | 2013-07-17 | 博瑞生物医药泰兴市有限公司 | Sterile culture detection device |
| CN103205359B (en) * | 2013-04-28 | 2014-04-16 | 博瑞生物医药泰兴市有限公司 | Sterile culture detection device |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20130925 Effective date of abandoning: 20140416 |
|
| RGAV | Abandon patent right to avoid regrant |