CN203136593U - Disposable petri dish applicable to rapid inoculation of ectomycorrhizal fungi in laboratory - Google Patents
Disposable petri dish applicable to rapid inoculation of ectomycorrhizal fungi in laboratory Download PDFInfo
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- CN203136593U CN203136593U CN 201320063064 CN201320063064U CN203136593U CN 203136593 U CN203136593 U CN 203136593U CN 201320063064 CN201320063064 CN 201320063064 CN 201320063064 U CN201320063064 U CN 201320063064U CN 203136593 U CN203136593 U CN 203136593U
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- petri dish
- box body
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- inoculation
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Abstract
The utility model discloses a disposable petri dish applicable to rapid inoculation of ectomycorrhizal fungi in a laboratory and relates to a disposable petri dish. By means of the disposable petri dish, the problem that ectomycorrhizae cannot be formed rapidly through prior petri dishes is solved. The disposable petri dish applicable to rapid inoculation of ectomycorrhizal fungi in the laboratory comprises a cover (1) and a box body (2), and the cover (1) covers the box body (2). The disposable petri dish is characterized in that a side casing of the cover (1) is made of an opaque black resin material, and the box body (2) is also made of the opaque black resin material. According to the disposable petri dish, the structure is simple, the utilization is convenient, and the ectomycorrhizae inoculation efficiency is improved rapidly. Compared with conventional ectomycorrhizae inoculation, the inoculation rate is improved by about 30% and the time is shortened by about 50% by the aid of the disposable petri dish.
Description
Technical field
The utility model relates to a kind of disposable culture dish, is specifically related to a kind of laboratory that is applicable to and inoculates the disposable culture dish of ectotrophic mycorrhiza fast.
Background technology
Employed disposable culture dish has many inconvenience as the device part of inoculation ectotrophic mycorrhiza in the process of using at present: (1) culture dish all is that transparent material is made, plant is exposed to the growth that is unfavorable for plant root under the full light at the inoculation back root part, also is unfavorable for forming fast simultaneously ectotrophic mycorrhiza; (2) do not have the perforate of reserving on lid and the box body, make plant stem can't be exposed to culture dish and carry out normal photosynthesis outward, unfavorable to the plant growth like this, can not form ectotrophic mycorrhiza as early as possible simultaneously.
The utility model content
The purpose of this utility model is in order to solve the problem that existing culture dish is unfavorable for forming fast ectotrophic mycorrhiza, and then provides a kind of laboratory that is applicable to inoculate the disposable culture dish of ectotrophic mycorrhiza fast.
The technical solution of the utility model is: a kind of laboratory that is applicable to is inoculated the disposable culture dish of ectotrophic mycorrhiza fast and is comprised lid and box body, cover cap is contained on the box body, the limit shell of lid is the resin material limit shell of black non transparent, and box body is the resin material box body of black non transparent.
Preferred version: have down gap on the limit shell of box body, have gap on the limit shell of lid, last gap is corresponding with following gap position and form a round hole jointly.
Preferred version: the diameter of round hole is 5mm.
Preferred version: the upper casing of lid is the transparent resin material upper casing.
The utility model compared with prior art has following effect: a culture dish of the present utility model is simple in structure, easy to use, improves ectotrophic mycorrhiza inoculation efficient fast.The lid upper casing is transparent, is convenient to observe the colony growth situation of ectotrophic mycorrhiza, generally inoculates when bacterium colony roughly covers with culture dish, can improve inoculation efficient like this, makes plant form ectotrophic mycorrhiza fast; Root must shading during plant inoculation ectotrophic mycorrhiza, uses the effectively shading of opaque black resin material, and several culture dishes constitute under one group the situation, and a upper casing to first culture dish carries out shading treatment and gets final product; Round hole on the culture dish is used for placing the stem of plant, and the stem leaves of plants part can not influence photosynthesis of plants at the culture dish outgrowth like this, and the well-grown of plant is conducive to form fast ectotrophic mycorrhiza.The rate of vaccination of the more conventional ectotrophic mycorrhiza of the utility model has improved about 30%, and the time has shortened about 50%.
Description of drawings
Fig. 1 is that the perspective view of the disposable culture dish of ectotrophic mycorrhiza is inoculated in a kind of laboratory that is applicable to of the utility model fast.
Embodiment
Embodiment one: present embodiment is described in conjunction with Fig. 1, a kind of laboratory that is applicable to of present embodiment is inoculated the disposable culture dish of ectotrophic mycorrhiza fast and is comprised lid 1 and box body 2, lid 1 lid is contained on the box body 2, the limit shell of lid 1 is the resin material limit shell of black non transparent, and box body 2 is the resin material box body of black non transparent.The resin material limit shell main purpose of the resin material box body of black non transparent and black non transparent is to be conducive to plant root to be in dark environment when the inoculation ectotrophic mycorrhiza, is beneficial to quick formation ectotrophic mycorrhiza.Have down gap 2-1 on the limit shell of box body 2, have gap 1-1 on the limit shell of lid 1, last gap 1-1 is corresponding with following gap 2-1 position and form a round hole jointly.The diameter of round hole is 5mm.Plant is in seeded process, and root is in the culture dish, and leaves and stems is in outside the culture dish, does not influence photosynthesis of plants like this, is conducive to the normal growth of plant and inoculates ectotrophic mycorrhiza fast.The upper casing of lid 1 is the transparent resin material upper casing, is convenient to observe the colony growth situation of ectotrophic mycorrhiza.
Use-case
Pinus sylvestris var. mongolica (Pinus sylvestris var.mongolica) inoculation suillusgranulatus (Suillus granulatus (L.:Fr.) O.Kuntce).The suillusgranulatus strain separating is from Heilongjiang Province's Fenglin Nature Reserve; bacterial strain is cultivated in the improvement comprehensive PDA culture medium; use the disposable culture dish of the utility model to cultivate ectotrophic mycorrhiza; colony diameter reaches 6cm after 2 weeks; the pinus sylvestris var. mongolica aseptic seedling is carried out inoculation experiments after cultivating for 4 weeks; the root of pinus sylvestris var. mongolica aseptic seedling is fitted on the bacterium colony of suillusgranulatus; enclose 2 of filter paper that soak into the deionized water of sterilizing above; cover lid; pinus sylvestris var. mongolica stem passes from the round hole of reserving, and with sealing film culture dish is sealed.The pinus sylvestris seedling of 2 week of inoculation back taking-up inoculation forms situation with body formula sem observation pinus sylvestris var. mongolica radicula surface mycelia sheath, by freezing microtome section technology microexamination Hartig net formation situation.The more traditional solid spawn rate of vaccination of the utility model improves 30%, time shortens 50%, traditional solid spawn inoculation method rate of vaccination reaches more than 90% and needs at least 60 days, and practical the utility model can reach rate of vaccination in 30 days above 90%, can go out the mycorhiza seedling in fast culture under the laboratory condition like this, for the research of mycorhiza is provided convenience.
Claims (4)
1. one kind is applicable to that the laboratory inoculates the disposable culture dish of ectotrophic mycorrhiza fast, and it comprises lid (1) and box body (2), and lid (1) lid is contained on the box body (2); It is characterized in that: the limit shell of lid (1) is the resin material limit shell of black non transparent, and box body (2) is the resin material box body of black non transparent.
2. the disposable culture dish of ectotrophic mycorrhiza is inoculated in a kind of laboratory that is applicable to according to claim 1 fast, it is characterized in that: have down gap (2-1) on the limit shell of box body (2), have gap (1-1) on the limit shell of lid (1), last gap (1-1) is corresponding with following gap (2-1) position and form a round hole jointly.
3. the disposable culture dish of ectotrophic mycorrhiza is inoculated in a kind of laboratory that is applicable to according to claim 2 fast, and it is characterized in that: the diameter of round hole is 5mm.
4. inoculate the disposable culture dish of ectotrophic mycorrhiza fast according to claim 1 or 3 described a kind of laboratories that are applicable to, it is characterized in that: the upper casing of lid (1) is the transparent resin material upper casing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320063064 CN203136593U (en) | 2013-02-04 | 2013-02-04 | Disposable petri dish applicable to rapid inoculation of ectomycorrhizal fungi in laboratory |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320063064 CN203136593U (en) | 2013-02-04 | 2013-02-04 | Disposable petri dish applicable to rapid inoculation of ectomycorrhizal fungi in laboratory |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203136593U true CN203136593U (en) | 2013-08-21 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201320063064 Expired - Fee Related CN203136593U (en) | 2013-02-04 | 2013-02-04 | Disposable petri dish applicable to rapid inoculation of ectomycorrhizal fungi in laboratory |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203136593U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107896690A (en) * | 2017-10-18 | 2018-04-13 | 内蒙古和盛生态科技研究院有限公司 | A kind of nutrient solution for promoting the fast quick-recovery of pinus sylvestris var. mongolica tree vigo(u)r |
-
2013
- 2013-02-04 CN CN 201320063064 patent/CN203136593U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107896690A (en) * | 2017-10-18 | 2018-04-13 | 内蒙古和盛生态科技研究院有限公司 | A kind of nutrient solution for promoting the fast quick-recovery of pinus sylvestris var. mongolica tree vigo(u)r |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130821 Termination date: 20150204 |
|
| EXPY | Termination of patent right or utility model |