CN202748306U

CN202748306U - Confocal optical scanner

Info

Publication number: CN202748306U
Application number: CN 201220235252
Authority: CN
Inventors: 赖博
Original assignee: Individual
Current assignee: Individual
Priority date: 2012-05-24
Filing date: 2012-05-24
Publication date: 2013-02-20
Anticipated expiration: 2022-05-24

Abstract

The utility model relates to a confocal optical scanner, in particular to a confocal optical scanner which uses array detectors such as a CCD (Charge Coupled Device), an EMCCD (Electron Multiplying Charge Coupled Devices), a CMOS (Complementary Metal-Oxide-Semiconductor Transistor), and an sCOMS (Service Customizable Overlay Multicast System) as a detecting unit and is applied to biological-fluorescence microscopic imaging. The confocal optical scanner is mainly characterized in that a lighting pinhole array and an imaging pinhole array are arranged in a light path, and pinholes arranged on the two arrays are in one-to-one correspondence; the imaging pinhole array is conjugate with the imaging focal plane of a microscope objective, only fluorescence emitted by a sample positioned on the focal plan of the microscope objective is transmitted, and light emitted by a light source is not reflected; and the scanning imaging to the sample is realized through the rotation of a scanning galvanometer with two reflecting surfaces. Thus, the confocal optical scanner has the advantages of high scanning speed, zero stray light and background noise, uniform lighting intensity and the like. Meanwhile, the scanning of the confocal optical scanner and the exposure of the detector are easy to realize synchronous control.

Description

A kind of confocal optical scanner

Technical field

The utility model relates to a kind of confocal optical scanner, particularly uses the planar array detectors such as CCD, EMCCD and CMOS as the burnt optical scanner of the copolymerization of detecting device.

Background technology

At present, the existing planar array detectors such as CCD, EMCCD and CMOS that use are as the burnt optical scanner of the copolymerization of detecting device, the main rotation of passing through single rotating disk of a plurality of printing opacity pin holes of arrangement, or arrange a plurality of printing opacity pin holes single sliding block reciprocatingly slide to realize scanning imagery to sample, the light direct irradiation of light source emission is on rotating disk or slide block, there is the light above 96% to be reflected by rotating disk or slide block, causes the high problem of parasitic light noise.By between light source and rotating disk or slide block, placing microlens array, can reduce rotating disk or slide block to the reflection of light source, but the microlens array manufacturing cost too high, be difficult to make and the problems such as size restrictions make it to be difficult to promote.

Summary of the invention

The utility model is for solving the high problem of parasitic light noise, and the technical scheme that adopts is: placed illumination pinhole array and imaging pinhole array in the light path, and these two pinhole array have identical pin hole arrangement mode, all pin holes are one-to-one relationship.The lightproof part of illumination pinhole array, the light reflection that does not see through pin hole with the light source emission makes it not enter imaging optical path, so avoided the high problem of parasitic light noise.Simultaneously, the utility model uses has the scanning galvanometer scanning samples of two reflectings surface, so have advantages of that sweep velocity is fast.

The utility model comprises as shown in Figure 1: light source 1; Exciter filter 2; Collimation lens set 3; Illumination pinhole array 4 has been arranged the pin hole 4a of a plurality of printing opacities on it; Dichroic beam splitter 5; Imaging pinhole array 6 is being arranged a plurality of printing opacity pin hole 6a on it; Delay lens group 7; Scanning galvanometer 8 and the first reflecting surface 8a and the second reflecting surface 8b; Total reflective mirror 9; Imaging lens group 10; Emission color filter 11; Controller 12.

The purpose of this utility model is achieved through the following technical solutions (such as Fig. 1):

The light of described light source 1 emission successively sees through described illumination pinhole array 4 and imaging pinhole array 6, and illumination is positioned at the sample 15 of object lens 14 focal planes of microscope 18; Arranged on described illumination pinhole array 4 and the described imaging pinhole array 6 one to one, pin hole 4a and the 6a of printing opacity, and the imaging focal plane conjugation of described imaging pinhole array 6 and micro objective 14; The fluorescence of described sample 15 emissions sees through the described pin hole 6a of described imaging pinhole array 6, quilt cover battle array detecting device 19 records; The rotation of the described scanning galvanometer 8 by having two reflectings surface scans described sample 15, realizes the confocal scanning imaging to described sample 15.

Described

pin hole

4a and 6a are shaped as circle or polygon.

The described first reflecting surface 8a of described scanning galvanometer 8 is that plane reflection or concave reflection, described the second reflecting surface 8b are plane reflection or concave reflection; Perhaps, described the first reflecting surface 8a and described the second reflecting surface 8b are respectively on two described scanning galvanometers 8.

Described exciter filter 2, be positioned at described light source 1 after, its role is to: see through the light of the part wavelength of described light source 1 emission, and stop the light of other wavelength.

Described collimation lens set 3 between described light source 1 and described illumination pinhole array 4, makes the light of described light source 1 emission form parallel beam; The optical axis of described collimation lens set 3 is perpendicular to described illumination pinhole array 4.

Described dichroic beam splitter 5 between described illumination pinhole array 4 and described imaging pinhole array 6, its role is to the light of described light source 1 emission and the fluorescence of described sample 15 emissions are split up into two light paths.

Described emission color filter 11 is positioned at before described the battle array detecting device 19, its role is to the fluorescence of only launching through described sample 15, the light that reflects other wavelength, makes described battle array detecting device 19 can only detect the fluorescence of described sample 15 emissions.

Described controller 12 has signal and communication with described battle array detecting device 19, by described scanning galvanometer 8 rotations of described controller 12 controls, make the time shutter of described battle array detecting device 19 equal the integral multiple of the rotational time of described scanning galvanometer 8, and to make the startup of described scanning galvanometer 8 rotations and the startup that described battle array detecting device 19 exposes be synchronization; In addition, the described light source 1 of described controller 12 controls makes 1 of described light source luminous when 19 exposure of described battle array detecting device.

The beneficial effects of the utility model are:

1, sweep velocity is fast, can realize quick co-focusing imaging.

2, avoid the parasitic light of slide block reflection, reduced ground unrest.

Description of drawings

Fig. 1: the structural representation of the utility model and microscope and face battle array detector applies

Fig. 2: pinhole array schematic diagram of the present utility model

Fig. 3: the schematic diagram of the second embodiment of the present utility model

Fig. 4: the schematic diagram of the third embodiment of the present utility model

Fig. 5: the schematic diagram of the 4th kind of embodiment of the present utility model

The drawing explanation:

Light source 1; Exciter filter 2; Collimation lens set 3; Illumination pinhole array 4 has been arranged the pin hole 4a of a plurality of printing opacities on it; Dichroic beam splitter 5; Imaging pinhole array 6 is being arranged a plurality of printing opacity pin hole 6a on it; Delay lens group 7; Scanning galvanometer 8 and the first reflecting surface 8a and the second reflecting surface 8b; Total reflective mirror 9; Imaging lens group 10; Emission color filter 11; Controller 12; Sensitive chip 13; Object lens 14; Sample 15; Computing machine 16; Display 17; Microscope 18; Face battle array detecting device 19.

Embodiment

Further describe the present invention below in conjunction with drawings and Examples.

Embodiment 1

Fig. 1 is the first confocal optical scanner schematic diagram relevant with the utility model, and Fig. 2 is the pinhole array schematic diagram of the present embodiment.Wherein, the first reflecting surface 8a of scanning galvanometer 8, the second reflecting surface 8b and total reflective mirror group 10 all are plane reflections, and pin hole 4a or pin hole 6a are shaped as circle; In addition, pin hole 4a or pin hole 6a also can be polygons, such as triangle, square, rectangle, quadrilateral, pentagon and hexagon etc.

In the present embodiment, when battle array detecting device 19 was unexposed face to face, controller 12 gated sweep galvanometers 8 stopped at forward or backwards maximum position of rotation, and it is not luminous to control simultaneously light source 1.

A battle array detecting device 19 begins exposure face to face, controller 12 control light sources 1 emissive lighting light, and simultaneously gated sweep galvanometer 8 begins clockwise or is rotated counterclockwise to the reverse or maximum spin angular position of forward from maximum spin angular position forward or backwards.Light is launched from light source 1, forms a plurality of shot point light sources through exciting the pin hole 6a on pin hole 4a, dichroic beam splitter 5 and the imaging pinhole array 6 on color filter 2, collimation lens set 3, the illumination pinhole array 4; The shot point light source forms lighting point through the sample 15 of the first reflecting surface 8a on the focal plane of the object lens 14 of microscope 18 of delay lens group 7 and scanning galvanometer 8, and lighting point is along with being rotated on the sample 15 of scanning galvanometer 8 is mobile.The fluorescence of lighting point excited sample 15 emissions is reflected by dichroic beam splitter 5 through the pin hole 6a of the first reflecting surface 8a, delay lens group 7 and the imaging pinhole array 6 of object lens 14, scanning galvanometer 8, the second reflecting surface 8b and emission color filter 11 through total reflective mirror group 9, imaging lens group 10, scanning galvanometer 8 is formed into picture point at the sensitive chip 13 of face battle array detecting device 19 again, and imaging point is along with mobile on the sensitive chip 13 of the face that the is rotated in battle array detecting device 19 of scanning galvanometer 8.

Face to face during battle array detecting device 19 end exposure, scanning galvanometer 8 just rotates to reverse or the maximum spin angular position of forward, sample 15 is once illuminated, and simultaneously sensitive chip 13 obtains the once complete fluorescence information of samples 15, and is processed, is shown as image at graphoscope 18 by computing machine 17.Could pass through the pin hole 6a of imaging pinhole array 6, reflected by dichroic beam splitter 5 and see through and launch color filter 11 arrival sensitive chips 13 because only be positioned at the fluorescence of sample 15 emission of the focal plane of object lens 14, so the image of demonstration is the Confocal Images of sample 15 on the graphoscope 17.

Embodiment 2

Fig. 3 is the structural representation of the second confocal optical scanner relevant with the utility model, specific as follows with the difference of embodiment 1: as to have increased the quantity of imaging lens group 10, made the image of sensitive chip 13 records and the imaging of 14 pairs of samples 15 of object lens can realize amplifying in strict 1: 1.

Embodiment 3

Fig. 4 is the structural representation of the third confocal optical scanner relevant with the utility model, specific as follows with the difference of embodiment 1: the first reflecting surface 8a and the second reflecting surface 8b, the total reflective mirror group 9 of scanning galvanometer 8 all are concave reflections, delay lens group 7 and imaging lens group 10 are not placed in the imaging effect of delay lens group 7 and imaging lens group 10 in the alternative light path in the light path.

Embodiment 4

Fig. 5 is the structural representation of the four kind confocal optical scanner relevant with the utility model, specific as follows with the difference of embodiment 3: the first reflecting surface 8a and the second reflecting surface 8b are respectively on two identical scanning galvanometers 8, by the rotation of controller 13 synchro control, reduced a slice total reflective mirror 9.

Claims

1. confocal optical scanner, the light of its light source emission is transmission illumination pinhole array and imaging pinhole array successively, and illumination is positioned at the sample of described microscopical focal plane of lens; Arranged on described illumination pinhole array and the described imaging pinhole array one to one, the pin hole of printing opacity, and the imaging focal plane conjugation of described imaging pinhole array and micro objective; The fluorescence of described sample emission sees through the described pin hole of described imaging pinhole array, quilt cover battle array detector recording; The rotation of the scanning galvanometer by having two reflectings surface scans described sample, realizes the confocal scanning imaging to described sample.

2. a kind of confocal optical scanner according to claim 1 is characterized in that, described pin hole be shaped as circle or polygon, such as triangle, square, rectangle, quadrilateral, pentagon and hexagon etc.

3. a kind of confocal optical scanner according to claim 1 is characterized in that: the first reflecting surface of described scanning galvanometer is that plane reflection or concave reflection, the second reflecting surface are plane reflection or concave reflection.

4. a kind of confocal optical scanner according to claim 3, it is characterized in that: described the first reflecting surface and described the second reflecting surface are respectively on two scanning galvanometers.

5. a kind of confocal optical scanner according to claim 1 also comprises a slice exciter filter, be positioned at described light source after, its role is to: see through the light of the part wavelength of described light source emission, and stop the light of other wavelength.

6. a kind of confocal optical scanner according to claim 1 also comprises one group of collimation lens set, between described light source and described illumination pinhole array, makes the light of described light source emission form parallel beam; The optical axis of described collimation lens set is perpendicular to described illumination pinhole array.

7. a kind of confocal optical scanner according to claim 1, also comprise a slice dichroic beam splitter, between described illumination pinhole array and described imaging pinhole array, its role is to the light of described light source emission and the fluorescence of described sample emission are split up into two light paths.

8. a kind of confocal optical scanner according to claim 1, also comprise a slice emission color filter, be positioned at before described the battle array detecting device, its role is to the fluorescence of only launching through described sample, the light that reflects other wavelength, make described battle array detecting device can only detect the fluorescence of described sample emission.

9. a kind of confocal optical scanner according to claim 1, comprise that also one has the controller of signal and communication with described battle array detecting device, by described controller gated sweep galvanometer rotation, make the time shutter of described battle array detecting device equal the integral multiple of the rotational time of described scanning galvanometer, and to make the startup of described scanning galvanometer rotation and the startup of described battle array detector exposure be synchronization.

10. a kind of confocal optical scanner according to claim 9, described controller is controlled described light source, makes described light source only luminous when described battle array detector exposure.