CN202715274U - Simple solid phase extraction (SPE) device - Google Patents
Simple solid phase extraction (SPE) device Download PDFInfo
- Publication number
- CN202715274U CN202715274U CN2012200462789U CN201220046278U CN202715274U CN 202715274 U CN202715274 U CN 202715274U CN 2012200462789 U CN2012200462789 U CN 2012200462789U CN 201220046278 U CN201220046278 U CN 201220046278U CN 202715274 U CN202715274 U CN 202715274U
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- CN
- China
- Prior art keywords
- solid phase
- phase extraction
- air inlet
- seal cover
- piston sheet
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000002414 normal-phase solid-phase extraction Methods 0.000 title claims abstract description 32
- 238000007789 sealing Methods 0.000 claims abstract description 26
- 239000000463 material Substances 0.000 claims description 13
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 8
- 239000007789 gas Substances 0.000 claims description 4
- 229910052757 nitrogen Inorganic materials 0.000 claims description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 3
- 239000000741 silica gel Substances 0.000 claims description 3
- 229910002027 silica gel Inorganic materials 0.000 claims description 3
- 229910001220 stainless steel Inorganic materials 0.000 claims description 3
- 239000010935 stainless steel Substances 0.000 claims description 3
- 230000008878 coupling Effects 0.000 claims description 2
- 238000010168 coupling process Methods 0.000 claims description 2
- 238000005859 coupling reaction Methods 0.000 claims description 2
- 238000003780 insertion Methods 0.000 claims description 2
- 230000037431 insertion Effects 0.000 claims description 2
- 239000007788 liquid Substances 0.000 abstract description 7
- 238000002203 pretreatment Methods 0.000 abstract description 2
- 238000000605 extraction Methods 0.000 description 8
- 239000012488 sample solution Substances 0.000 description 8
- 239000000523 sample Substances 0.000 description 5
- 239000002904 solvent Substances 0.000 description 5
- 238000000034 method Methods 0.000 description 4
- 238000013461 design Methods 0.000 description 2
- 238000010828 elution Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000000945 filler Substances 0.000 description 2
- 238000012856 packing Methods 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 239000004480 active ingredient Substances 0.000 description 1
- 239000012491 analyte Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005755 formation reaction Methods 0.000 description 1
- 238000001172 liquid--solid extraction Methods 0.000 description 1
- 239000006210 lotion Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000005245 sintering Methods 0.000 description 1
- 238000004454 trace mineral analysis Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
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Abstract
The utility model relates to a simple solid phase extraction (SPE) device applied to sample pre-treatment. The simple SPE device comprises an SPE small column, a sealing cover and an air inlet device, wherein the sealing cover is covered at the inlet of the SPE small column; the air inlet device comprises a rubber pipette bulb, a rubber tube, a rubber tube interface and an air inlet tube; the rubber pipette bulb is sleeved on the rubber tube; the rubber tube is sleeved on the air inlet tube through the rubber tube interface; a hollow sealing ring is arranged below the sealing cover; the diameter of the sealing ring is the same as the shape and size of the inlet of the SPE small column; the sealing cover is provided with a large hole and a small hole; the large hole is taken as a liquid filling hole; the small hole is taken as an air inlet hole; the bottom surface of the liquid filling hole of the sealing cover is provided with a piston sheet; the size of the piston sheet is greater than the diameter of the liquid filling hole of the sealing cover, and is smaller than the diameter of the SPE small column; a half of the piston sheet is fixed below the liquid filling hole; the other parts of the piston sheet are movable; the air inlet tube is inserted into the small hole; and one tenth of the circumference of the left side of the piston sheet is bonded on the sealing cover.
Description
Technical field
The utility model relates to a kind of solid-phase extraction device of sample pre-treatments, relate in particular to a kind of can be quickly and easily to single or a plurality of trace analysis things separate, the simple and easy liquid-solid extraction device of purifying.
Background technology
At present, the most frequently used solid-phase extraction device in laboratory is made of SPE (SPE) pillar and auxiliary.The SPE pillar is comprised of three parts such as column jecket, sintering pad and fillers.Auxiliary generally has the formations such as vacuum system, vavuum pump, inertia source of the gas, sampler and surge flask.The SPE pillar can be changed according to kind difference, the quantity of analyte various dissimilar, and slow owing to SPE pillar rate of extraction in extraction process, need to by the pressure-acting pick up speed of auxiliary, raise the efficiency.But existing auxiliary device assembling is complicated, needs vacuum hydro-extraction, and material is required height, and loss is large, and complex operation is not suitable for some quick, simple extraction experiments of laboratory.
Summary of the invention
In order to solve the problems of the technologies described above, the utility model solves the technical scheme that its technical problem adopts and is: the structure of solid-phase extraction device has the SPE pillar, also comprises a seal cover, and inlet duct, seal cover cover the entrance at the SPE pillar.
Further inlet duct comprises rubber pipette bulb, rubber tube, and the rubber tube interface, air inlet pipe, rubber pipette bulb is socketed on the rubber tube, and rubber tube is socketed on the air inlet pipe by the rubber tube interface.
The sealing ring that the below of further seal cover has a hollow, the diameter of sealing ring is identical with the shape size of the entrance of SPE pillar, at seal cover small one and large one two holes are arranged, wherein macropore is filling opening, aperture is air inlet, in the filling opening bottom surface of seal cover a piston sheet is arranged, the size of piston sheet is greater than the filling opening diameter of seal cover, piston sheet is semifixed in the filling opening below, remainder can be movable, aperture is used for inserting air inlet pipe, and the size of air inlet is identical with the appearance and size of air inlet pipe insertion portion.
1/10 circumference in further preferred piston sheet left side sticks on the seal cover.
Further have clip on the seal cover, be used for fixing and clamp seal cover and SPE pillar.
Further sealing ring is the hollow sealing circle, and its diameter is identical with the inlet diameter of SPE pillar, and it is wide to be 1.0mm, the high 3.0mm of being.
The material of further preferred seal cover and sealing ring is quality of rubber materials, and the material of circular piston sheet is the silica gel material.Adopt such material corrosion resistance strong, the elasticity of material itself, frictional force is good.
Further preferred air inlet pipe is a lower thin and upper thick stainless steel tube, the butt end of air inlet pipe up, taper end is inserted in the air inlet, the length of taper end is 20mm, butt end is used for overlapping the rubber tube interface of coupling, connects gas source.
Cuff rubber pipette bulb or Nitrogen evaporator on the further air inlet pipe.Adopt such structure to can be implemented in the SPE pillar to add single or multiple samples and process.
In the SPE pillar, add sample solution in sum, cover a kind of seal cover of Simple solid phase extraction device, and use clamp, airtight.If the processing of single sample, directly the rubber pipette bulb of cuff rubber tube on air inlet pipe pressurizes to the SPE pillar with rubber pipette bulb; If the processing of a plurality of samples can be overlapped Nitrogen evaporator in air inlet pipe, directly with the nitrogen pressurization, at this moment the piston sheet of filling opening is because the reaction of pressure is in sealing state.Simultaneously, piston sheet also plays the effect of a surge flask, and when the rubber pipette bulb resorption, piston sheet is opened, and can prevent the liquid suck-back in the SPE pillar.All behind the little column packing, stop pressurization by SPE when sample solution, piston sheet is in active state, and eluting solvent can directly inject from filling opening with liquid-transfering gun or small syringe, repeats above-mentioned pressure process again, carries out wash-out.The design of piston sheet has been satisfied simultaneously sealing, surge flask and has been added the requirement of three aspects such as washing lotion, simply, rapidly the SPE pillar is carried out repeatedly wash-out, reaches the purpose that reduces the sample loss of effective components.And when carrying out the extraction of another sample, a part that only needs to change seal cover reaches the cross pollution that prevents solvent, reduces the wastage, and reduces experimentation cost.By normal pressure, finish easily and quickly the extraction process of single or a plurality of samples simultaneously, can carry out repeatedly elution process again simultaneously, and can prevent cross pollution, simplify experiment, easy to operate, few loss.
Description of drawings
Fig. 1 is the main cutaway view that a kind of Simple solid phase extraction device seal cover engages with air inlet pipe;
Fig. 2 is the top view of Fig. 1;
Fig. 3 is a kind of schematic diagram of Simple solid phase extraction device;
Among the figure: 1 is air inlet pipe, and 2 is air inlet, and 3 is filling opening, and 4 is piston sheet, and 5 is sealing ring, and 6 is clip, and 7 is the rubber tube interface, and 8 is rubber pipette bulb, and 9 is rubber tube, and 10 is the SPE pillar, and 11 is seal cover.
The specific embodiment
Below in conjunction with drawings and Examples the utility model is further specified.
Among Fig. 1 and Fig. 2: take SPE pillar 10 entrances as shape, rubber is material, be seal cover 11 of SPE pillar 10 designs, its thickness is 2.0mm, and with two clip 6 fixing seals lid 11 and SPE pillar 10, and for the sealing of enhanced leaktightness lid 11, there is a hollow sealing circle 5 onesize with the little column inlet of SPE the lower end of device, the diameter 12.0mm of sealing ring, wide 1.0mm, high 3.0mm.On the seal cover small one and large one two holes are arranged simultaneously, macropore is filling opening 3, the diameter of filling opening is selected according to the diameter of SPE pillar, in the present embodiment because the SPE pillar is the standard component of 6ml, so preferred 3.5mm of the diameter of filling opening, and do a circular piston sheet 4 of being made by the silica gel material in the bottom, diameter 6.5mm, piston sheet 4 left sides 1/10 circumference is sticked on the seal cover, and remainder is movable, and covers filling opening 3; Aperture is air inlet 2, diameter 2.0mm, and simultaneously, one one is thin, thin diameter 2.0mm, long 20.0mm; One is pluckings, and its diameter 8.0mm, the stainless steel tube of long 12.0mm are air inlet pipe 1, the air inlet 2 of taper end insert-seal lid, and butt end is furnished with the rubber tube interface 7 of a straight-through G 1/8, connects gas source with this.
In the embodiment shown in fig. 3, when single sample solution is extracted, open first a kind of seal cover 11 of Simple solid phase extraction device, add sample solution from SPE pillar opening part, cover seal cover 11, and with clip 6 clampings, sealing, the rubber tube 9 on the rubber pipette bulb 8 is accessed rubber tube interface 7 upper ends that match.After finishing above-mentioned steps, begin slowly to pressurize with 8 pairs of SPE pillars 11 of rubber pipette bulb, piston sheet 4 is closed, and the sample solution in the SPE pillar 11 slowly oozes.If once the outflow of all samples solution is not finished in pressurization, can discharge rubber pipette bulb 8, at this moment piston sheet 4 is opened because of the counter-pressure effect, can not cause liquid suck-back in the SPE pillar.And then continue pressurization, until sample solution is all by the little column packing of SPE, thereby finished the pressure extraction process of sample solution.Next to carry out to SPE pillar and filler 2-3 time solvent elution, reduce the loss of sample active ingredient.Do not having under the pressure-acting, piston sheet 4 can easily be opened, extract a certain amount of solvent with liquid-transfering gun and be directly injected into filling opening 3, in the situation that does not need the device for opening seal cover, finish the liquid feeding process of eluting solvent, and then repeat above-mentioned pressure extraction step, namely can reach the separation of single sample solution, the purpose of purifying.
Above embodiment is a kind of preferred realization of the present utility model, and the utility model is not consisted of special restriction.The change that those skilled in the art carry out on this basis and modification still belong to protection domain of the present utility model.
Claims (9)
1. Simple solid phase extraction device, its structure comprises solid phase extraction column, it is characterized in that: its structure also comprises a seal cover, and inlet duct, seal cover cover the entrance at solid phase extraction column.
2. a kind of Simple solid phase extraction device according to claim 1, it is characterized in that: inlet duct comprises rubber pipette bulb, rubber tube, the rubber tube interface, air inlet pipe, rubber pipette bulb is socketed on the rubber tube, and rubber tube is socketed on the air inlet pipe by the rubber tube interface.
3. a kind of Simple solid phase extraction device according to claim 1 and 2 is characterized in that: the below of seal cover has the sealing ring of a hollow, the diameter of sealing ring is identical with the shape size of the entrance of solid phase extraction column, at seal cover small one and large one two holes are arranged, wherein macropore is filling opening, aperture is air inlet, in the filling opening bottom surface of seal cover a piston sheet is arranged, the size of piston sheet is greater than the filling opening diameter of seal cover, diameter less than solid phase extraction column, piston sheet is semifixed in the filling opening below, remainder can be movable, and aperture is used for inserting air inlet pipe, and the size of air inlet is identical with the appearance and size of air inlet pipe insertion portion.
4. a kind of Simple solid phase extraction device according to claim 3 is characterized in that: 1/10 circumference in piston sheet left side sticks on the seal cover.
5. a kind of Simple solid phase extraction device according to claim 3 is characterized in that: have clip on the seal cover, be used for fixing and clamp seal cover and solid phase extraction column.
6. a kind of Simple solid phase extraction device according to claim 3, it is characterized in that: sealing ring is the hollow sealing circle, and its diameter is identical with the inlet diameter of solid phase extraction column, and it is wide to be 1.0mm, highly be 3.0mm.
7. a kind of Simple solid phase extraction device according to claim 3, it is characterized in that: the material of seal cover and sealing ring is quality of rubber materials, the material of circular piston sheet is the silica gel material.
8. a kind of Simple solid phase extraction device according to claim 3, it is characterized in that: air inlet pipe is a lower thin and upper thick stainless steel tube, and the butt end of air inlet pipe is up, taper end is inserted in the air inlet, the length of taper end is 20mm, and butt end is used for overlapping the rubber tube interface of coupling, connects gas source.
9. a kind of Simple solid phase extraction device according to claim 3 is characterized in that: cuff rubber pipette bulb or Nitrogen evaporator on the air inlet pipe.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012200462789U CN202715274U (en) | 2012-02-14 | 2012-02-14 | Simple solid phase extraction (SPE) device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012200462789U CN202715274U (en) | 2012-02-14 | 2012-02-14 | Simple solid phase extraction (SPE) device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202715274U true CN202715274U (en) | 2013-02-06 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012200462789U Expired - Fee Related CN202715274U (en) | 2012-02-14 | 2012-02-14 | Simple solid phase extraction (SPE) device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202715274U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103191586A (en) * | 2013-04-23 | 2013-07-10 | 陈浩达 | Chromatographic column assembly |
| CN106422417A (en) * | 2016-11-23 | 2017-02-22 | 百奥森（江苏）食品安全科技有限公司 | Novel solid-phase extraction column capable of performing suction under negative pressure |
| CN114019039A (en) * | 2021-10-13 | 2022-02-08 | 温州医科大学附属第二医院（温州医科大学附属育英儿童医院） | Method for selectively extracting phospholipid from plasma sample and phospholipid detection method |
-
2012
- 2012-02-14 CN CN2012200462789U patent/CN202715274U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103191586A (en) * | 2013-04-23 | 2013-07-10 | 陈浩达 | Chromatographic column assembly |
| CN106422417A (en) * | 2016-11-23 | 2017-02-22 | 百奥森（江苏）食品安全科技有限公司 | Novel solid-phase extraction column capable of performing suction under negative pressure |
| CN114019039A (en) * | 2021-10-13 | 2022-02-08 | 温州医科大学附属第二医院（温州医科大学附属育英儿童医院） | Method for selectively extracting phospholipid from plasma sample and phospholipid detection method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130206 Termination date: 20140214 |