CN103920306B - A kind of pressue device for nano-fiber solid phase extraction column - Google Patents
A kind of pressue device for nano-fiber solid phase extraction column Download PDFInfo
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- CN103920306B CN103920306B CN201410148184.6A CN201410148184A CN103920306B CN 103920306 B CN103920306 B CN 103920306B CN 201410148184 A CN201410148184 A CN 201410148184A CN 103920306 B CN103920306 B CN 103920306B
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Abstract
The invention discloses a kind of pressue device for nano-fiber solid phase extraction column, this pressue device comprises housing, bottom, shell fragment and pressured column in a tubular form, housing is provided with the dead slot paralleled with the axis of housing, and bottom is provided with through hole, and bottom is fixedly connected on the bottom of housing; Shell fragment is fixedly connected on housing; The outer surface of pressured column is provided with the draw-in groove be parallel to each other, and pressured column is arranged in housing, and the draw-in groove of pressured column matches with the bottom of shell fragment.This pressue device solves nano-fiber solid phase extraction column and crosses in post process, the problem that the manual operations that may occur when pressure is excessive is wasted time and energy.
Description
Technical field
The present invention relates to a kind of pressue device, relate in particular to a kind of pressue device for nano-fiber solid phase extraction column.
Background technology
When carrying out analysis to biological sample and detecting, sample composition is very complicated, and interfering material is many, and testing concentration is general all very low, and therefore, but Sample pretreatment is the most complicated, a loaded down with trivial details the most key step all the time.SPE is the one separation method fast and effectively based on liquid chromatogram separation principle.By sample solution by being pre-charged with the pillar of Stationary liquid, separated component is retained by forms such as absorption, distribution, ion-exchanges, then uses suitable solvent elution, to reach the object of separation, enrichment and elite.This researcher takes the lead in carrying out with electro spinning nano fiber analyzing sample pre-treatments research, is that adsorbent loads solid-phase extraction column with nanofiber.Because electro spinning nano fiber has high specific area, obviously increase with the interactional site of target molecule, adsorption and desorption speed is fast, compared with existing extraction stuffing, nanofiber solid phase extraction techniques Extraction and enrichment efficiency significantly improves, the consumption of amount of samples and eluant, eluent all greatly reduces, if but containing the stickum such as mucopolysaccharide, protein in sample, column pressure will be increased, extended the post time, simple operation is by hand wasted time and energy and also may not reached the effect of pressurization, and this device comes therefrom.
Summary of the invention
technical problem:technical problem to be solved by this invention is: provide a kind of pressue device for nano-fiber solid phase extraction column, and this pressue device solves nano-fiber solid phase extraction column and crosses in post process, the problem that the manual operations that may occur when pressure is excessive is wasted time and energy.
technical scheme:for solving the problems of the technologies described above, the technical solution used in the present invention is:
A kind of pressue device for nano-fiber solid phase extraction column, it is characterized in that, this pressue device comprises housing, bottom, shell fragment and pressured column in a tubular form, and housing is provided with the dead slot paralleled with the axis of housing, bottom is provided with through hole, and bottom is fixedly connected on the bottom of housing; Shell fragment is fixedly connected on housing; The outer surface of pressured column is provided with the draw-in groove be parallel to each other, and pressured column is arranged in housing, and the draw-in groove of pressured column matches with the bottom of shell fragment.
Further, the described pressue device for nano-fiber solid phase extraction column, also comprises fastener, fastener is arranged in housing, the side of fastener 6 passes the dead slot of housing 1, and matches with the draw-in groove of pressured column in the upper end of fastener, matches with the push rod top of solid-phase extraction column in the lower end of fastener.
Further, the internal face of described bottom is provided with internal thread, and the bottom wall of housing is provided with external screw thread, and the internal thread of bottom and the external screw thread of housing match.
Further, middle part and the top of described shell fragment are positioned at hull outside, and the top of shell fragment is positioned at above housing, and the middle part of shell fragment is connected in the outside wall surface of housing by Flexible Connector.
Further, the bottom of shell fragment is through the dead slot of housing, and the bottom of shell fragment tilts to the axis of housing.
Further, the diameter on the top of described pressured column is greater than the diameter of housing cavity.
Further, the draw-in groove of described pressured column and the axis perpendicular of pressured column, and the distance between adjacent two draw-in grooves is 3 to 7 millimeters.
Further, the top of described shell fragment is provided with suppending hole.
Further, described Flexible Connector comprises screw and spring, and spring housing is contained on the root of screw, and the bottom of screw is embedded in the housing.
Further, the outer surface of described housing is provided with the fin be parallel to each other.
beneficial effect:compared with prior art, technical scheme of the present invention has following beneficial effect:
1. cross post speed controlled.Cross the extraction efficiency impact of post speed on SPE very large, when crossing post speed, object not easily adsorbs, and time slower, lose time and requires great effort.Pressue device of the present invention arranges the draw-in groove of some on pressured column surface, and when pressured column presses down, a certain draw-in groove of pressured column blocks fixing shell fragment, plays pressured column positioning action, and then controls the pressure in pillar, thus controls post speed.When shell fragment matches with the draw-in groove of pressured column diverse location, pressured column varies in size to solid-phase extraction column applied pressure, thus controls the size of post speed.
2., when pressure is excessive, can be easy to apply pressure.During for viscous samples, such as saliva sample, the saliva sample of 0.5mL crosses post process often needs 10 minutes-30 minutes.Prior art normally uses hand solid-phase extraction column, until sample is excessively complete, wastes time and energy very much.Pressue device of the present invention stops the mode of pressured column resilience by shell fragment, does not need artificially to apply pressure in the process waiting for post, utilizes the suppending hole on shell fragment to be suspended on and waits on one side.
3. convenient manual operation, without the need to providing power, being applicable to various occasion and using.Prior art such as the patent No. is the array solid-phase extraction device based on nanofiber absorption of CN 200910033559, when carrying out the sample detection of negligible amounts (1 or 2), inconvenient with this array.Pressue device convenient manual operation of the present invention, without the need to providing power, being applicable to various occasion and using.The use of existing solid-phase extraction column all needs the devices such as aspiration pump usually, to aspirate or pressuring method operation, needs extra power to supply power.Pressue device structure of the present invention is easier, more convenient operation.
Accompanying drawing explanation
Fig. 1 is structural representation of the present invention.
Fig. 2 is the structural representation of pressured column in the present invention.
Have in figure: housing 1, bottom 2, shell fragment 3, pressured column 4, suppending hole 5, fastener 6, draw-in groove 7.
Detailed description of the invention
Below in conjunction with accompanying drawing, technical scheme of the present invention is described in detail.
As shown in Figure 1 to Figure 2, a kind of pressue device for nano-fiber solid phase extraction column of the present invention, comprise housing 1 in a tubular form, bottom 2, shell fragment 3 and pressured column 4, housing 1 is provided with the dead slot paralleled with the axis of housing 1, bottom 2 is provided with through hole, and bottom 2 is fixedly connected on the bottom of housing 1; Shell fragment 3 is fixedly connected on housing 1; The outer surface of pressured column 4 is provided with the draw-in groove 7 be parallel to each other, and pressured column 4 is arranged in housing 1, and the draw-in groove 7 of pressured column 4 matches with the bottom of shell fragment 3.
When using the pressue device for nano-fiber solid phase extraction column of said structure, solid-phase extraction column is positioned at above bottom 2, and bottom 2 is for bottling up solid-phase extraction column.Solid-phase extraction column, by the dead slot on housing 1 sidewall, enters in housing 1.Bottom 2 is connected to the bottom of housing 1.Shell fragment 3 is fixedly connected on housing 1.Pressured column 4 moves down from the top end opening of housing 1.Match with the draw-in groove 7 on pressured column 4 in the bottom of shell fragment 3.When dismantling solid-phase extraction column, bottom 2 being unloaded from housing 1, then solid-phase extraction column is taken out from the dead slot housing 1 sidewall.Pressue device of the present invention be especially applicable to nano-fiber solid phase extraction column with the use of, because nano-fiber solid phase extraction column is especially applicable to the process of biological sample, amount of samples is few, is generally several milliliters, generally adding sample just can processing sample completely, can be excessively complete by sample easily.
Further, the described pressue device for nano-fiber solid phase extraction column, also comprises fastener 6, fastener 6 is arranged in housing 1, the side of fastener 6 passes the dead slot of housing 1, and matches with the draw-in groove 7 of pressured column 4 in the upper end of fastener 6, matches with the push rod top of solid-phase extraction column in the lower end of fastener 6.By arranging fastener 6, the push rod of pressured column 4 with solid-phase extraction column is coupled together.When pressured column 4 is pulled back, the push rod of solid-phase extraction column is also pulled back with pressured column 4.The side of fastener 6 passes the dead slot of housing 1, is convenient to the installation and removal of fastener 6.
Further, the internal face of described bottom 2 is provided with internal thread, and the bottom wall of housing 1 is provided with external screw thread, and the internal thread of bottom 2 and the external screw thread of housing 1 match.Bottom 2 is fixedly connected with mode with housing 1 to be had multiple, such as, adopt clamping.This patent is preferably threaded.By arranging internal thread and external screw thread, make the installation and removal between bottom 2 and housing 1 very convenient, and the fixing that both connect is high.
Further, middle part and the top of described shell fragment 3 are positioned at outside housing 1, and the top of shell fragment 3 is positioned at above housing 1, and the middle part of shell fragment 3 is connected in the outside wall surface of housing 1 by Flexible Connector.Shell fragment 3 is arranged on outside housing 1, is convenient to the installation of shell fragment 3.By arranging Flexible Connector, when push shrapnel 3 top time, the bottom of shell fragment 3 can tilt, and the upper and lower direction of motion of shell fragment 3 is contrary.Push shrapnel 3 top, the bottom of shell fragment 3 can be made to depart from the draw-in groove 7 of pressured column 4, pressured column 4 of now can pulling back.Flexible Connector can adopt various structures, the structure below the present invention preferably: Flexible Connector comprises screw and spring, and spring housing is contained on the root of screw, and the bottom of screw is embedded within the case 1.
Further, the bottom of described shell fragment 3 is through the dead slot of housing 1, and the bottom of shell fragment 3 tilts to the axis of housing 1.Arrange shell fragment 3 bottom to tilt to the axis of housing 1, make shell fragment 3 bottom can be good at coordinating with the draw-in groove 7 of pressured column 4.
Further, the diameter on the top of described pressured column 4 is greater than the diameter of housing 1 inner chamber.Like this, pressured column 4 can not all enter in housing 1.Meanwhile, the tip size of pressured column 4 is comparatively large, is convenient to people and exerts a force by hand.
Further, the draw-in groove 7 of described pressured column 4 and the axis perpendicular of pressured column 4, and the distance between adjacent two draw-in grooves 7 is 3 to 7 millimeters.The draw-in groove 7 of fair amount and spacing is set, is conducive to controlling pressured column 4 pairs of solid-phase extraction columns and applies pressure size, control to exert a force precision at every turn.
Further, the top of described shell fragment 3 is provided with suppending hole 5.Suppending hole 5 is for hanging whole pressue device.
Further, the outer surface of described housing 1 is provided with the fin be parallel to each other.Like this, when people holds the fin of housing 1, not easily skid, increase the fixing held.
Exemplify an embody rule example below.
Extract the object in saliva: put into from the top end opening of housing 1 by the pressured column 4 of nano-fiber solid phase extraction column, pressured column 4 is inserted, the nano-fiber solid phase extraction column after activation is added by getting supernatant after centrifugal for 1mL saliva, the pusher section of solid-phase extraction column is connected by fastener 6 with forcing pipe 4, through hole on bottom 2 was overlapped solid-phase extraction column, after external screw thread bottom the internal thread of bottom 2 and housing 1 is tightened, pressured column 4 is pressed downwards from pressured column 4 top, when pressured column 4 in position time, when producing certain pressure, be fixed on the bottom of the shell fragment 3 of upper end, Handheld Division to match with the draw-in groove 7 on pressured column 4, can realize adding required pressure to crossing post liquid.Matching with the draw-in groove 7 on pressured column 4 in the bottom of shell fragment 3, makes when not having External Force Acting, and pressured column 4 can not move along its axis.The draw-in groove 7 on a pressured column 4 is blocked in shell fragment 3 bottom, ensures that pressured column 4 up can not rebound because of the pressure of nano-fiber solid phase extraction column, often blocks a groove and also can provide different pressure for solid-phase extraction column, regulate sample to cross the speed of post with this.Crossing in post process, the suppending hole 5 on shell fragment 3 can also be utilized, whole pressue device is hung up, does not need hand-held.After sample has been crossed, back out screw-cap, taken off nano-fiber solid phase extraction column, add elution for subsequent analysis operation.
Claims (7)
1. the pressue device for nano-fiber solid phase extraction column, it is characterized in that, this pressue device comprises housing (1), bottom (2), shell fragment (3) and pressured column (4) in a tubular form, housing (1) is provided with the dead slot paralleled with the axis of housing (1), bottom (2) is provided with through hole, and bottom (2) is fixedly connected on the bottom of housing (1); Shell fragment (3) is fixedly connected on housing (1); The outer surface of pressured column (4) is provided with the draw-in groove (7) be parallel to each other, pressured column (4) is arranged in housing (1), and the draw-in groove (7) of pressured column (4) matches with the bottom of shell fragment (3);
Also comprise fastener (6), fastener (6) is arranged in housing (1), the side of fastener (6) passes the dead slot of housing (1), and match with the draw-in groove (7) of pressured column (4) in the upper end of fastener (6), match with the push rod top of solid-phase extraction column in the lower end of fastener (6);
The middle part of described shell fragment (3) and top are positioned at housing (1) outside, and the top of shell fragment (3) is positioned at housing (1) top, the middle part of shell fragment (3) is connected to by Flexible Connector in the outside wall surface of housing (1); The bottom of shell fragment (3) is through the dead slot of housing (1), and the bottom of shell fragment (3) tilts to the axis of housing (1).
2. according to the pressue device for nano-fiber solid phase extraction column according to claim 1, it is characterized in that, the internal face of described bottom (2) is provided with internal thread, the bottom wall of housing (1) is provided with external screw thread, and the internal thread of bottom (2) and the external screw thread of housing (1) match.
3. according to the pressue device for nano-fiber solid phase extraction column according to claim 1, it is characterized in that, the diameter on the top of described pressured column (4) is greater than the diameter of housing (1) inner chamber.
4. according to the pressue device for nano-fiber solid phase extraction column according to claim 1, it is characterized in that, the draw-in groove (7) of described pressured column (4) and the axis perpendicular of pressured column (4), and the distance between adjacent two draw-in grooves (7) is 3 to 7 millimeters.
5. according to the pressue device for nano-fiber solid phase extraction column according to claim 1, it is characterized in that, the top of described shell fragment (3) is provided with suppending hole (5).
6. according to the pressue device for nano-fiber solid phase extraction column according to claim 1, it is characterized in that, described Flexible Connector comprises screw and spring, and spring housing is contained on the root of screw, and the bottom of screw is embedded in housing (1).
7. according to the pressue device for nano-fiber solid phase extraction column according to claim 1, it is characterized in that, the outer surface of described housing (1) is provided with the fin be parallel to each other.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410148184.6A CN103920306B (en) | 2014-04-15 | 2014-04-15 | A kind of pressue device for nano-fiber solid phase extraction column |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410148184.6A CN103920306B (en) | 2014-04-15 | 2014-04-15 | A kind of pressue device for nano-fiber solid phase extraction column |
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| CN103920306B true CN103920306B (en) | 2015-11-04 |
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| CN105344131A (en) * | 2015-11-18 | 2016-02-24 | 河北中烟工业有限责任公司 | Manual solid phase extraction device |
| CN111443074B (en) * | 2020-05-11 | 2021-08-31 | 厦门大学 | Automatic on-line pretreatment and Raman detection device and method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101590333A (en) * | 2009-06-23 | 2009-12-02 | 东南大学 | Array solid-phase extraction device based on nanofiber absorption |
| CN103191584A (en) * | 2013-04-26 | 2013-07-10 | 苏州东奇生物科技有限公司 | Portable solid phase extracting device |
| CN203842349U (en) * | 2014-04-15 | 2014-09-24 | 东南大学 | Pressurizing device for nanofiber solid-phase extraction column |
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- 2014-04-15 CN CN201410148184.6A patent/CN103920306B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101590333A (en) * | 2009-06-23 | 2009-12-02 | 东南大学 | Array solid-phase extraction device based on nanofiber absorption |
| CN103191584A (en) * | 2013-04-26 | 2013-07-10 | 苏州东奇生物科技有限公司 | Portable solid phase extracting device |
| CN203842349U (en) * | 2014-04-15 | 2014-09-24 | 东南大学 | Pressurizing device for nanofiber solid-phase extraction column |
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