CN202543201U - Microarray dialysis chamber - Google Patents
Microarray dialysis chamber Download PDFInfo
- Publication number
- CN202543201U CN202543201U CN 201220166716 CN201220166716U CN202543201U CN 202543201 U CN202543201 U CN 202543201U CN 201220166716 CN201220166716 CN 201220166716 CN 201220166716 U CN201220166716 U CN 201220166716U CN 202543201 U CN202543201 U CN 202543201U
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- cover plate
- micropore
- lower cover
- plate
- microwell array
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
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Abstract
The utility model discloses a microarray dialysis chamber, which is characterized by comprising an upper cover plate, a lower cover plate and a central plate, wherein the central plate is clamped and fixed between the upper cover plate and the lower cover plate, micropore arrays are arranged on the upper cover plate, the lower cover plate and the central plate respectively and are strictly and vertically aligned, and each micropore in the micropore arrays on the central plate is isolated into an independent dialysis chamber by a first micropore filtering membrane and a second micropore filtering membrane. Compared with the prior art, the microarray dialysis chamber has the advantages that on one hand, high-throughput cultivation of microorganisms can be ensured by a plurality of micropores on the central plate, and on the other hand, appropriate amount of strains with different dominance hierarchies can be implanted into each micropore on the central plate and can be cultivated through independent cultivation of each micropore, monoclonal cultivation is further and approximately acquired, and the separation efficiency is simultaneously improved.
Description
Technical field
The utility model relates to a kind of device of culturing micro-organisms, specifically is meant a kind of microarray dialysate chamber.
Background technology
Marine microorganism distributes extensively, quantity is many, and it is special in Marine ecosystems, to play a part, and the mikrobe in the ocean environment more than 99% can't obtain through conventional separation and Culture, all can't grow in case leave former environment.Existing research shows; These " but non-culturing micro-organisms " play the important and pivotal role in ecotope and earth energy and material cycle; Therefore, the cultural method of research and development " but non-culturing micro-organisms " all is the focus of microbiological research with technology all the time.
At present, the researchist has carried out original position separation " but non-culturing micro-organisms " Study on Technology, and wherein, the dialysate chamber culture technique is one of its main culture technique, correspondingly, multiple dialysate chamber culture apparatus also occurred.Like the patent No. is the Chinese utility model patent disclosed " a kind of two-way dialyzer " of ZL 200620120442.0 (Granted publication number for CN 2937146Y); This dialyzer is processed with stainless steel, is shaped as rectangle, it is characterized in that being made up of three parts; Wherein, Left and right side room is the nutrient media storage chamber, and intermediate chamber is a bacteria growth chamber, about two Room and intermediate chamber separate with the dialysis mould.This two-way dialyzer has enlarged dialysis face through two-way dialysis; Thereby the nutritive substance in substratum is fully utilized, and still, this two-way dialyzer is not suitable in situ environment and cultivates; Because the nutrient media storage chamber in this two-way dialyzer is in the relative closure state; In culturing process, being positioned at the indoor nutritive substance of nutrient media storage can reduce gradually, and culture efficiency must be influenced.As improvement to the dialysate chamber of this relative closure; People have invented many open dialysate chamber devices that can be suitable for the in situ environment cultivation; But these dialysate chamber devices all have only a single dialysate chamber, make once can only cultivate a duplicate samples, and culture efficiency is lower.And; The diameter of these dialysate chambers generally is centimetre-sized, and the dialysate chamber volume is bigger, and is more in the bacterial strain quantity that a dialysate chamber is implanted into; These bacterial strains of implanting same dialysate chamber generally comprise the bacterial classification of different dominance hierarchies; In culturing process, the process survival of the fittest between the bacterial classification of different dominance hierarchies in the dialysate chamber, dominant bacteria wherein is able to existence and cultivates; Inferior advantage bacterial classification wherein and non-dominant bacteria are difficult to because of growth is suppressed cultivate, and promptly are difficult to obtain the mono-clonal cultivation of time advantage bacterial classification and non-dominant bacteria.In addition,, in culturing process, cultivate the dominant bacteria that comes out and often also divide quite a few kinds, these dominant bacterias are separated, operate comparatively loaded down with trivial details thereby also must pass through follow-up lock out operation because bacterial strain quantity is more in the dialysate chamber.
The utility model content
The utility model technical problem to be solved is to above-mentioned prior art present situation, provides a kind of flux of cultivating higher and can obtain to be similar to the microarray dialysate chamber of mono-clonal cultivation.
The utility model solves the problems of the technologies described above the technical scheme that is adopted: this microarray dialysate chamber; It is characterized in that: comprise that upper cover plate, lower cover and folder establish the center plate that is fixed between the upper and lower cover plate; On said upper and lower cover plate and center plate, be respectively equipped with the microwell array of strict alignment up and down; Between the microwell array of the microwell array of upper cover plate and center plate, be folded with first millipore filtration; Between the microwell array of the microwell array of lower cover and center plate, be folded with second millipore filtration, each micropore in the microwell array of said center plate is isolated into independently dialysate chamber by described first millipore filtration and second millipore filtration.
Further preferred, the aperture of each micropore is 1～2mm in the said microwell array.Certainly, corresponding adjustment can be done according to the experiment difference in the aperture of micropore in the microwell array, can implant an amount of bacterial strain quantity in each micropore of center plate and cultivates as long as make.
Further preferred; Described upper and lower cover plate and center plate are rectangle; Length direction is respectively equipped with two described microwell arrays separately in upper and lower cover plate and center plate upper edge; And described microwell array is circular, and accordingly, described first millipore filtration and second millipore filtration also are circle.Like this, whole apparatus structure is comparatively simple, also is convenient to adopt simultaneously standardized millipore filtration.
As the improvement of such scheme, the diameter of said microwell array is 25mm, in said first millipore filtration in the aperture of each micropore and said second millipore filtration aperture of each micropore be 0.1 μ m.Wherein, The micropore size of millipore filtration can be done corresponding adjustment according to the experiment difference; But what need guarantee implantable dialysis chamber waits that cultivating the bacterial strain bacterial strain outer with being positioned at dialysate chamber all can not see through millipore filtration, and makes small molecules nutrition mass-energy through millipore filtration and the dialysate chamber of freely coming in and going out simultaneously.
As the further improvement of such scheme, all have circular groove at the upper surface of said upper cover plate and the lower surface of lower cover, the microwell array on the said upper and lower cover plate takes shape in the bottom of the circular groove that belongs to cover plate separately respectively.After groove was set, the integral thickness of upper and lower cover plate can increase, thereby improved apparatus structure intensity.
In order further to improve structural strength, described upper and lower cover plate and center plate all adopt the polyoxymethylene plate.
Compared with prior art; The advantage of the utility model is: because each micropore in the center plate microwell array of this microarray dialysate chamber forms independently dialysate chamber under millipore filtration is isolated; At first, the numerous micropores in the microwell array of center plate can be guaranteed the high-throughput cultivation of mikrobe; Secondly; Because the aperture of the micropore of center plate is less, in each micropore, can put into an amount of bacterial strain and cultivate, dominant bacteria possibly only be present in the part dialysate chamber wherein; Another part dialysate chamber wherein possibly only contain time advantage bacterial classification and non-dominant bacteria; These times advantage bacterial classification and non-dominant bacteria can not receive the inhibition of dominant bacteria in culturing process, so through after the whole microarray dialysate chamber cultivation, can be similar to the mono-clonal that obtains various different dominance hierarchy bacterial classifications and cultivate; At last and since each independently dialysate chamber generally only turn out the single culture of certain dominance hierarchy, make that the later separation operation is comparatively simple, separation efficiency is higher.
Description of drawings
Fig. 1 is the perspective view of the utility model embodiment;
Fig. 2 is the vertical view (removing screw) of microarray dialysate chamber shown in Figure 1;
Fig. 3 is the three-dimensional exploded view of microarray dialysate chamber shown in Figure 1.
Embodiment
Embodiment describes in further detail the utility model below in conjunction with accompanying drawing.
To shown in Figure 3, the microarray dialysate chamber in the present embodiment comprises upper cover plate 1, lower cover 2 and presss from both sides establishes the center plate 3 that is fixed between the upper and lower cover plate like Fig. 1.Wherein, upper cover plate 1, lower cover 2 and center plate 3 are rectangle, and the size of upper cover plate 1 and lower cover 2 is 80*40*8mm, and center plate 3 is of a size of 80*40*2mm.At the lower surface of the upper surface of upper cover plate 1 and lower cover 2 along the circular groove 6 that to be evenly equipped with two diameters be 25mm of length direction separately; Form circular microwell array 4 in the bottom of above-mentioned circular groove 6, the diameter of above-mentioned circular microwell array 4 is 25mm or is slightly less than 25mm; Length direction at center plate 3 is evenly equipped with two circular microwell arrays 4, and the diameter of this two microwell array 4 also is 25mm or be slightly less than 25mm, and these two microwell arrays 4 that are positioned at center plate 3 and the microwell array that is positioned at upper and lower cover plate about in the of 4 strictness align.Certainly, upper and lower cover plate and center plate are not limited to above-mentioned size, can adjust slightly as required.Folder is established first millipore filtration 51 between the microwell array 4 of the microwell array 4 of center plate 3 and upper cover plate 1; Folder is established second millipore filtration 52 between the microwell array 4 of the microwell array 4 of center plate 3 and lower cover 2; First millipore filtration 51 and diameter identical with second millipore filtration, 52 structures is 25mm; Under the isolation of first millipore filtration 51 and second millipore filtration 52, each micropore in the center plate 3 is formed an independently dialysate chamber.
The diameter of existing dialysate chamber generally is centimetre-sized, adopts existing dialysate chamber to cultivate bacterial strain, implantable a large amount of bacterial strain in the dialysate chamber; Often not only include dominant bacteria but also comprise time advantage bacterial classification and non-dominant bacteria in these a large amount of bacterial strains; So the bacterial classification of above-mentioned different dominance hierarchies is cultivated in a bigger space, each bacterial classification is through after selecting the superior and eliminating the inferior in culturing process; Dominant bacteria is able to existence and is cultivated out; And inferior advantage bacterial classification and non-dominant bacteria are suppressed and be difficult to cultivate, so last cultivation results is that the mono-clonal that can only obtain dominant bacteria is cultivated.Cultivate in order to implant an amount of bacterial strain in each micropore that makes center plate 3; The aperture of the micropore of center plate 3 and each micropore of upper and lower cover plate generally is below the 5mm; Be preferably 1～2mm, in the present embodiment, the aperture of above-mentioned each micropore is 1mm; When making diluted sample concentration reach nearest inoculum density, the bacterial strain quantity of implanting in each dialysate chamber of center plate 3 is about 5.Like this; Dominant bacteria possibly only be present in the part dialysate chamber wherein; Another part dialysate chamber wherein possibly only contain time advantage bacterial classification and non-dominant bacteria; These times advantage bacterial classification and non-dominant bacteria can not receive the inhibition of dominant bacteria in culturing process, so through after the whole microarray dialysate chamber cultivation, the mono-clonal that can obtain the bacterial classification of various different dominance hierarchies is cultivated.And, since each independently dialysate chamber generally only turn out the single culture of certain dominance hierarchy, make that the later separation operation is comparatively simple, separation efficiency is higher.
In addition; Wait that for what make the implantable dialysis chamber cultivating the bacterial strain bacterial strain outer with being positioned at dialysate chamber all can not see through millipore filtration, and make small molecules nutrition mass-energy through millipore filtration and the dialysate chamber of freely coming in and going out simultaneously, in the present embodiment; The aperture of each micropore is 0.1 μ m in the millipore filtration 5; Certainly, according to the different experiments needs, can select to use the millipore filtration 5 in different apertures.In the present embodiment; A microwell array 4 on the center plate 3 has 192 micropores, and promptly center plate 3 microwell array has 192 independently dialysate chambers, thereby has guaranteed the high-throughput cultivation; Certainly, the quantity of micropore also is not limited to 192 in center plate 3 microwell array.
For make upper and lower cover plate and center plate closely clamping be one; In the present embodiment; On upper and lower cover plate and center plate, all have one group of open hole 8, every group of open hole is six, four mounting hole site wherein in around; Two other mounting hole site is in the centre; The corresponding aperture position up/down perforation of these three groups of open holes after six screws 7 pass corresponding open hole 8 as fastening piece fastens as one upper and lower cover plate and center plate, thereby makes each micropore of center plate 3 form an independently dialysate chamber.In addition, in order to improve the structural strength of this microarray dialysate chamber, in the present embodiment, upper and lower cover plate and center plate all adopt structural strength polyoxymethylene plate preferably.
The installation process of this microarray dialysate chamber is following: earlier bacterial strain to be cultivated is inoculated in each dialysate chamber of center plate; Then upper cover plate 1, first millipore filtration 51, center plate 3, second millipore filtration 52 and lower cover 2 are fastened as one up and down through screw from top to bottom, the device that will fasten as one at last places in situ environment or simulation in situ environment to cultivate.
Claims (6)
1. microarray dialysate chamber; It is characterized in that: comprise upper cover plate (1), lower cover (2) and press from both sides and establish the center plate (3) that is fixed between the upper and lower cover plate; On said upper and lower cover plate (1,2) and center plate (3), be respectively equipped with the microwell array (4) of strict alignment up and down; Between the microwell array of the microwell array of upper cover plate (1) and center plate (3), be folded with first millipore filtration (51); Between the microwell array of the microwell array of lower cover (2) and center plate (3), be folded with second millipore filtration (52), each micropore in the microwell array of said center plate (3) is isolated into independently dialysate chamber by described first millipore filtration (51) and second millipore filtration (52).
2. microarray dialysate chamber according to claim 1 is characterized in that: the aperture of each micropore is 1～2mm in the said microwell array (4).
3. microarray dialysate chamber according to claim 1 and 2; It is characterized in that: described upper and lower cover plate (1,2) and center plate (3) are rectangle; Length direction is respectively equipped with two described microwell arrays (4) separately in upper and lower cover plate (1,2) and center plate (3) upper edge; And described microwell array (4) is circular, and accordingly, described first millipore filtration (51) and second millipore filtration (52) also are circle.
4. microarray dialysate chamber according to claim 3 is characterized in that: the diameter of said microwell array (4) is 25mm, in said first millipore filtration (51) in the aperture of each micropore and said second millipore filtration (52) aperture of each micropore be 0.1 μ m.
5. microarray dialysate chamber according to claim 1 and 2; It is characterized in that: all have circular groove (6) at the upper surface of said upper cover plate (1) and the lower surface of lower cover (2), the microwell array (4) on the said upper and lower cover plate (1,2) takes shape in the bottom of the circular groove (6) that belongs to cover plate separately respectively.
6. microarray dialysate chamber according to claim 1 and 2 is characterized in that: described upper and lower cover plate (1,2) and center plate (3) all adopt the polyoxymethylene plate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220166716 CN202543201U (en) | 2012-04-18 | 2012-04-18 | Microarray dialysis chamber |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220166716 CN202543201U (en) | 2012-04-18 | 2012-04-18 | Microarray dialysis chamber |
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| CN202543201U true CN202543201U (en) | 2012-11-21 |
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| CN 201220166716 Withdrawn - After Issue CN202543201U (en) | 2012-04-18 | 2012-04-18 | Microarray dialysis chamber |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102634447A (en) * | 2012-04-18 | 2012-08-15 | 浙江万里学院 | Micro-array dialysis chamber and enrichment culture method using same |
| CN103865751A (en) * | 2012-12-17 | 2014-06-18 | 中国科学院上海生命科学研究院 | Array-type high-throughput microbe separating culturing apparatus |
| CN104789449A (en) * | 2015-04-27 | 2015-07-22 | 国家***第一海洋研究所 | In-situ culturing device for deep-sea microbes |
| CN105255712A (en) * | 2015-10-12 | 2016-01-20 | 国家***第一海洋研究所 | High-throughput microorganism in-situ culture device |
| CN105969637A (en) * | 2016-06-17 | 2016-09-28 | 国家***第海洋研究所 | Deep sea microorganism in-situ cultivation and enrichment device |
| CN107118966A (en) * | 2017-05-05 | 2017-09-01 | 李宜芳 | In situ environment microorganism separation method, the separation of soil origin oil degradation microorganism and screening technique |
-
2012
- 2012-04-18 CN CN 201220166716 patent/CN202543201U/en not_active Withdrawn - After Issue
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102634447A (en) * | 2012-04-18 | 2012-08-15 | 浙江万里学院 | Micro-array dialysis chamber and enrichment culture method using same |
| CN102634447B (en) * | 2012-04-18 | 2013-09-18 | 浙江万里学院 | Micro-array dialysis chamber and enrichment culture method using same |
| CN103865751A (en) * | 2012-12-17 | 2014-06-18 | 中国科学院上海生命科学研究院 | Array-type high-throughput microbe separating culturing apparatus |
| CN103865751B (en) * | 2012-12-17 | 2015-11-18 | 中国科学院上海生命科学研究院 | A kind of array high throughput separation and Culture antimicrobial device |
| CN104789449A (en) * | 2015-04-27 | 2015-07-22 | 国家***第一海洋研究所 | In-situ culturing device for deep-sea microbes |
| CN105255712A (en) * | 2015-10-12 | 2016-01-20 | 国家***第一海洋研究所 | High-throughput microorganism in-situ culture device |
| CN105969637A (en) * | 2016-06-17 | 2016-09-28 | 国家***第海洋研究所 | Deep sea microorganism in-situ cultivation and enrichment device |
| CN107118966A (en) * | 2017-05-05 | 2017-09-01 | 李宜芳 | In situ environment microorganism separation method, the separation of soil origin oil degradation microorganism and screening technique |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20121121 Effective date of abandoning: 20130918 |
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| RGAV | Abandon patent right to avoid regrant |