CN202849409U - Cell co-culture bottle - Google Patents
Cell co-culture bottle Download PDFInfo
- Publication number
- CN202849409U CN202849409U CN 201220547224 CN201220547224U CN202849409U CN 202849409 U CN202849409 U CN 202849409U CN 201220547224 CN201220547224 CN 201220547224 CN 201220547224 U CN201220547224 U CN 201220547224U CN 202849409 U CN202849409 U CN 202849409U
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- China
- Prior art keywords
- culture
- cell
- culturing room
- bottleneck
- cells
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/58—Reaction vessels connected in series or in parallel
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/08—Chemical, biochemical or biological means, e.g. plasma jet, co-culture
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Clinical Laboratory Science (AREA)
- General Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Cell Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to a cell co-culture bottle which is mainly used for solving the problems of small size, complexity in operation, low cell yield and incapability of recycle commonly existing in the current co-culture device. The cell co-culture bottle is provided with two culture rooms which are separated through a microfiltration membrane, and each culture room is provided with a culture bottle opening. The cell co-culture bottle has the advantages of larger size, high cell yield and capable of meeting the research requirement for a great number of proteins and the like; and due to the adoption of the structure, the culture bottle is more unique in operation and can be recycled, and the cost is reduced.
Description
Technical field
The utility model relates to a kind of coenecium culture apparatus, relates in particular to that a kind of volume is large, handled easily, the co-culture of cells bottle that can reuse.
Background technology
The interaction of cell and microorganism is that organic sphere generally occurs.The common culturing room research cell of cell or microorganism and the basic means of microbial interaction.Early stage scientific research with single cell or single microorganism as research, the research of cell interaction is less, the research of cell and microbial interaction is also less.Along with the increase of the depth of investigation, these interact and in fact to belong to exchanging between information interchange between a kind of intercellular information interchange, the microorganism and cell and the microorganism.This information interchange all has a great impact microorganism and cell, affects their propagation, differentiation, material secretion.
Because interactional search time is longer, the research of direct interaction is difficulty comparatively.Because different cell co-cultivations, different cell growth cycles are different, and probably the grow living space of the another kind of cell of too fast contention of a kind of cell is not easy to observe; The inconvenience of what is more important mixed culture cell is distinguished, and causes cell in-situ to be identified and has certain difficulty.What is particularly worth mentioning is that, cell and microorganism are difficult to cultivate altogether, because mixed culture inevitably causes necrocytosis, research can't be carried out.By contrast, the Indirect Interaction of iuntercellular or cell and microorganism then is conducive to scientific research personnel's discovery information interchange therebetween, has also found a lot of important phenomenon, and for preventing and curing diseases, the biology shape of control cell and microorganism is for providing strategy.
Co-culture of cells is an important method of research cell-cell interaction, and co-culture device ubiquity volume is little at present, complicated operation, and cell yield is few, and the research such as a large amount of albumen of can not satisfying the demand requirement can not be recycled with price high.
Summary of the invention
It is little that the utility model has mainly solved present co-culture device ubiquity volume, complicated operation, and cell yield is few, and the problem that can not reuse provides a kind of volume large, simple to operate, and cell yield is large, the co-culture of cells bottle that can reuse.
The technical scheme that its technical problem that solves the utility model adopts is: a kind of co-culture of cells bottle, this culturing bottle have two culturing room, are separated by by millipore filtration between two culturing room, are respectively arranged with the cultivation bottleneck in each culturing room.The utility model separates by middle millipore filtration, allows cell culture fluid freely to come in and go out, and is applicable to the foundation of the co-culture system of attached cell, suspension cell and half suspension cell.The utility model can carry out separately the cell cultures operation by cultivating bottleneck, and is simple to operate, mutual contamination of cells not, and can reuse, reduced cost.
As a kind of preferred version, described millipore filtration is penetrating film, and the membrane pore size of millipore filtration is less than 50 microns.This millipore filtration aperture arranges and allows nutrient solution freely to enter, but does not allow the cell turnover.
As a kind of preferred version, the size of described culturing room is 5ml-5L.The utility model culturing room volume is large, and cell yield is large, the research such as a large amount of albumen of can satisfying the demand requirement.
As a kind of preferred version, described millipore filtration is organic materials or inorganic materials.
As a kind of preferred version, described culturing room is square chamber.
As a kind of preferred version, the described bottleneck chamber that quadrangular frustum pyramid shaped is arranged respectively on a side of each culturing room, described bottleneck chamber communicates with culturing room, and described bottleneck is arranged on the end face of bottleneck chamber truncated rectangular pyramids structure.The structure of bottleneck chamber is convenient to operation, more easily pours into and pours out nutritive medium.
The utility model has the advantages that: the culturing bottle volume is larger, and cell yield is large, the research such as a large amount of albumen of can satisfying the demand requirement; The culturing bottle structure and can reuse so that operation is more independent, has reduced cost.
Description of drawings
Fig. 1 is a kind of structural representation of the present utility model.
The 2-of 1-culturing room millipore filtration 3-bottleneck chamber 4-cultivates bottleneck
Embodiment
Below by embodiment, and by reference to the accompanying drawings, the technical solution of the utility model is further described.
Embodiment:
A kind of co-culture of cells bottle of the present embodiment as shown in Figure 1, is provided with millipore filtration 2 in this culturing bottle, it is square that this millipore filtration is divided into 1, two culturing room of two culturing room with culturing bottle, and culturing room's size is 5L, and the aperture of this millipore filtration is 40 microns.Culturing bottle also includes two bottleneck chambers, and two bottleneck chambers are the truncated rectangular pyramids structure, and the bottleneck chamber is separately positioned on the side of culturing room, with culturing room's intracavity inter-connection, is provided with cultivation bottleneck 4 at bottleneck chamber front end face.
This culturing bottle is not limited only to two culturing room, can culturing bottle be separated into 4 or more culturing room by millipore filtration as required, is provided with the bottleneck chamber on the side of each culturing room and cultivates bottleneck.
Specific embodiment described herein only is to the explanation for example of the present invention's spirit.Those skilled in the art can make various modifications or replenish or adopt similar mode to substitute described specific embodiment, but can't depart from spirit of the present invention or surmount the defined scope of appended claims.
Although this paper has more used the terms such as culturing room, millipore filtration, bottleneck chamber, cultivation bottleneck, do not get rid of the possibility of using other term.Using these terms only is in order to describe more easily and explain essence of the present invention; They are construed to any additional restriction all is contrary with spirit of the present invention.
Claims (6)
1. co-culture of cells bottle, it is characterized in that: this culturing bottle has two culturing room (1), is separated by by millipore filtration (2) between two culturing room, is respectively arranged with in each culturing room and cultivates bottleneck (4).
2. a kind of co-culture of cells bottle according to claim 1 is characterized in that described millipore filtration (2) is penetrating film, and the membrane pore size of millipore filtration is less than 50 microns.
3. a kind of co-culture of cells bottle according to claim 1 and 2, the size that it is characterized in that described culturing room (1) is 5ml-5L.
4. a kind of co-culture of cells bottle according to claim 1 and 2 is characterized in that described millipore filtration (2) is organic materials or inorganic materials.
5. a kind of co-culture of cells bottle according to claim 1 is characterized in that described culturing room (1) is square chamber.
6. according to claim 1 and 2 or 5 described a kind of co-culture of cells bottles, it is characterized in that the described bottleneck chamber (3) that quadrangular frustum pyramid shaped is arranged respectively on a side of each culturing room (1), described bottleneck chamber communicates with culturing room, and described cultivation bottleneck (4) is arranged on the end face of bottleneck chamber truncated rectangular pyramids structure.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220547224 CN202849409U (en) | 2012-10-23 | 2012-10-23 | Cell co-culture bottle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220547224 CN202849409U (en) | 2012-10-23 | 2012-10-23 | Cell co-culture bottle |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202849409U true CN202849409U (en) | 2013-04-03 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201220547224 Expired - Lifetime CN202849409U (en) | 2012-10-23 | 2012-10-23 | Cell co-culture bottle |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202849409U (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105324478A (en) * | 2013-08-08 | 2016-02-10 | 学校法人金泽医科大学 | Culture vessel |
| CN106103691A (en) * | 2014-04-14 | 2016-11-09 | 株式会社岛津制作所 | Cell is cultivated with equipment, cell cultivation system and cell culture processes |
| CN106754358A (en) * | 2016-12-23 | 2017-05-31 | 青岛农业大学 | A kind of co-culture of cells blake bottle of convenient transfer cell |
| CN110438007A (en) * | 2019-07-25 | 2019-11-12 | 天津大学 | Many cells system culture bottle |
| WO2021038038A1 (en) * | 2019-08-30 | 2021-03-04 | Deutsches Krebsforschungszentrum | Multi-cell culture device and kit |
| CN114525198A (en) * | 2022-01-21 | 2022-05-24 | 江苏海洋大学 | Microalgae non-contact co-culture device |
-
2012
- 2012-10-23 CN CN 201220547224 patent/CN202849409U/en not_active Expired - Lifetime
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105324478A (en) * | 2013-08-08 | 2016-02-10 | 学校法人金泽医科大学 | Culture vessel |
| CN105324478B (en) * | 2013-08-08 | 2018-05-18 | 学校法人金泽医科大学 | Culture vessel |
| US10253291B2 (en) | 2013-08-08 | 2019-04-09 | Kanazawa Medical University | Culture vessel |
| CN106103691A (en) * | 2014-04-14 | 2016-11-09 | 株式会社岛津制作所 | Cell is cultivated with equipment, cell cultivation system and cell culture processes |
| US10301586B2 (en) | 2014-04-14 | 2019-05-28 | Shimadzu Corporation | Cell culturing device, cell culturing system and cell culturing method |
| CN106754358A (en) * | 2016-12-23 | 2017-05-31 | 青岛农业大学 | A kind of co-culture of cells blake bottle of convenient transfer cell |
| CN106754358B (en) * | 2016-12-23 | 2023-06-30 | 青岛农业大学 | Cell co-culture bottle convenient for transferring cells |
| CN110438007A (en) * | 2019-07-25 | 2019-11-12 | 天津大学 | Many cells system culture bottle |
| WO2021038038A1 (en) * | 2019-08-30 | 2021-03-04 | Deutsches Krebsforschungszentrum | Multi-cell culture device and kit |
| CN114525198A (en) * | 2022-01-21 | 2022-05-24 | 江苏海洋大学 | Microalgae non-contact co-culture device |
| CN114525198B (en) * | 2022-01-21 | 2022-12-06 | 江苏海洋大学 | Microalgae non-contact co-culture device |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20130403 |