CN202222239U - Carbon dioxide incubator - Google Patents
Carbon dioxide incubator Download PDFInfo
- Publication number
- CN202222239U CN202222239U CN2011202153997U CN201120215399U CN202222239U CN 202222239 U CN202222239 U CN 202222239U CN 2011202153997 U CN2011202153997 U CN 2011202153997U CN 201120215399 U CN201120215399 U CN 201120215399U CN 202222239 U CN202222239 U CN 202222239U
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- carbon dioxide
- co2gas incubator
- incubator
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- Cultivation Of Plants (AREA)
Abstract
The utility model relates to a plant tissue culturing device, in particular to a carbon dioxide incubator. The carbon dioxide incubator comprises a carbon dioxide gas cylinder, an artificial climate box with a carbon dioxide sensor inside as well as a flow controller connected with the carbon dioxide gas cylinder and the artificial climate box; a solenoid valve is arranged in the flow controller, and the input of the carbon dioxide is controlled through on/off of the solenoid valve. According to the utility model, the accuracy control of concentration of carbon dioxide in the incubator can be realized through a carbon dioxide through-put capacity adjusting device, and the additionally-applied carbon dioxide enables the carbon dioxide in a container to be enriched, so that tissue culture seedlings grow strong, and have high survival rates.
Description
Technical field
The utility model relates to the plant tissue culture device, more specifically, relates to a kind of CO2gas incubator.
Background technology
In plant tissue culture course, envirment factor is to the great influence of having grown of tissue cultivating seedling.Traditional Plant Tissue Breeding mode, for preventing to pollute the container that adopts seal very strong, with certain light and shade cycle constant temperature culture, tissue cultivating seedling leans on the sugar in the medium to carry out heterotrophic growth under certain illumination condition.Environmental difference inside and outside the container is very big, and humidity is higher in the culture vessel, and gas fluidity is poor, and carbonic acid gas is not enough, and secondary metabolism noxious material concentration is higher, and microbial contamination is serious.These all can directly cause on the tissue cultivating seedling form and physiological variation, as poor growth, be prone to the glass seedling and emerge after environmental suitability difference etc. to external world.
The utility model content
The purpose of the utility model provides a kind of CO2gas incubator, and it is poor to overcome the gas fluidity that has the sugar cultivation to cause in the prior art in the plant tissue culture process, and carbonic acid gas is not enough, and secondary metabolism noxious material concentration is higher, the problem that microbial contamination is serious.
In order to achieve the above object; The utility model provides a kind of CO2gas incubator, and said CO2gas incubator comprises the flow regulator that dioxide bottle, inside are equipped with the climatic cabinate of carbon dioxide sensor and are connected said dioxide bottle and climatic cabinate.
Wherein, have magnetic valve in the said flow regulator, control the input of carbonic acid gas through the opening and closing of magnetic valve.
Wherein, said flow regulator has the concentration set parts, is used to preestablish the experiment desired concn;
When the gas concentration lwevel in the said climatic cabinate is higher than the predefined experiment desired concn of said concentration set parts, closed electromagnetic valve; When the gas concentration lwevel in the said climatic cabinate was lower than the predefined experiment desired concn of said concentration set parts, magnetic valve was opened.
Wherein, have plant tissue culture vessel in the said climatic cabinate, this container is to be processed by polyethylene resin film.
The CO2gas incubator that the utility model provided; Pass through quantity regulating device through carbonic acid gas; Realization is to the accurate control of gas concentration lwevel in the incubator; Increase and execute carbonic acid gas and make the carbonic acid gas in the container be enrichment state, this environment that more approaches nature can make tissue cultivating seedling grow more healthy and stronglyer, and survival rate is higher.
Description of drawings
Fig. 1 is the structural representation of the utility model CO2gas incubator.
Among the figure: 1, dioxide bottle; 2, climatic cabinate; 3, flow regulator.
Embodiment
Following examples are used to explain the utility model, but are not used for limiting the scope of the utility model.
The utility model provides a kind of CO2gas incubator, and said CO2gas incubator comprises the dioxide bottle 1 and climatic cabinate 2 that carbon dioxide is housed, and the flow regulator 3 that connects said dioxide bottle and climatic cabinate.Said climatic cabinate 2 inside are equipped with carbon dioxide sensor and are used for the density of carbon dioxide gas in the induction-box.Can on carbon dioxide flow adjuster 3, set the required gas concentration lwevel of experiment; The concentration that carbon dioxide flow adjuster 3 records according to carbon dioxide sensor; Utilize the input of its inner solenoid control carbonic acid gas; When gas concentration lwevel in the case is higher than setting concentration, closed electromagnetic valve; Otherwise magnetic valve is opened until the concentration of arrive setting, and its ERROR CONTROL is within ± 40ppm scope.
Have the container that is used for tissue culture in the said climatic cabinate, this container is to utilize polyethylene resin film to process.With the fluoroethylene resin is that the fluoroethylene resin film that raw material is processed has superior resistance to elevated temperatures (can carry out autoclaving) and highly-breathable, high transmission rate, low-moisture permeability and is easy to characteristics such as processing and forming.Thereby the fluoroethylene resin film is the material culture vessel, and this container will have: light transmittance height, good permeability, poisture-penetrability is low and characteristics such as chemical resistance corrosion.In addition; After adopting this container to accomplish the explant inoculation, can use sealing machine directly to carry out hot-press sealed at opening of container, this mode will greatly reduce contamination by micro probability in the test-tube plantlet incubation; Former because in medium, do not add sugar simultaneously as carbon; Greatly reduce the topmost pollution problem that occurs in the group training, the reduction of pollution rate directly brings the decline of production cost, also helps preserving simultaneously the rare species material.
Traditional group training matrix is agar, and in the process that tissue cultivating seedling is emerged, cleaning agar is a very time-consuming process of taking a lot of work, and cleans the transplanting survival rate that totally can not reduce tissue cultivating seedling, especially under the situation of large-scale production, can increase production cost greatly.Mineral wool block is a modern container nursery matrix commonly used, replaces agar with mineral wool block, can directly be transplanted to the supporting cave dish from tissue cultivating seedling, thereby reduce the production cost of nursery stock.The utility model satisfies the illumination demand of different plant tissue culture seedling optimum growh states through regulating light quality and intensity of illumination.
Adopt the culture effect of the utility model CO2gas incubator:
With chrysanthemum (eastern woods timely snow) is example, uses sugar-free culture-medium, the artificial CO2 that adds; CO2 concentration is made as 3000ppm, with normal condition (medium MS+ sucrose 30g/L+ agar 6g/L, temperature: 25 ℃; Light intensity: 3000Lx, light application time: 12h/d, environment gas concentration lwevel 800ppm) as contrast.Use MS to be medium, the pH value is adjusted to 5.8.Adopt polyflon film culture vessel and 350ml vial as culture vessel.To supply the every container of examination material to inoculate 16 strains, 3 containers are respectively inoculated in four kinds of processing of each kind, totally 48 strains.Each handles actual measurement strain number is 15 strains.Twice of repeated test.
Conclusion is following: the cultivation results of CO2gas incubator significantly is superior to conventional the cultivation on plant height, the number of sheets, radical, root length, fresh weight, dry weight index.
Table 1 adopts the cultivation situation of CO2gas incubator
Above embodiment only is used to explain the utility model; And be not the restriction to the utility model; The those of ordinary skill in relevant technologies field under the situation of spirit that does not break away from the utility model and scope, can also be made various variations; Therefore all technical schemes that are equal to also belong to the category of the utility model, and the scope of patent protection of the utility model should be limited its claim.
Claims (5)
1. a CO2gas incubator is characterized in that, said CO2gas incubator comprises the flow regulator that dioxide bottle, inside are equipped with the climatic cabinate of carbon dioxide sensor and are connected said dioxide bottle and climatic cabinate.
2. CO2gas incubator as claimed in claim 1 is characterized in that, has magnetic valve in the said flow regulator, controls the input of carbonic acid gas through the opening and closing of magnetic valve.
3. CO2gas incubator as claimed in claim 2 is characterized in that, said magnetic valve is set to when the gas concentration lwevel in the said climatic cabinate is higher than the experiment desired concn, closed electromagnetic valve; When the gas concentration lwevel in the said climatic cabinate was lower than the experiment desired concn, magnetic valve was opened and is charged into carbonic acid gas.
4. CO2gas incubator as claimed in claim 3 is characterized in that said flow regulator has the concentration set parts, is used to preestablish the experiment desired concn.
5. like each described CO2gas incubator of claim 1-4, it is characterized in that having plant tissue culture vessel in the said climatic cabinate, this container is processed by polyethylene resin film.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011202153997U CN202222239U (en) | 2011-06-23 | 2011-06-23 | Carbon dioxide incubator |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011202153997U CN202222239U (en) | 2011-06-23 | 2011-06-23 | Carbon dioxide incubator |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202222239U true CN202222239U (en) | 2012-05-23 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011202153997U Expired - Lifetime CN202222239U (en) | 2011-06-23 | 2011-06-23 | Carbon dioxide incubator |
Country Status (1)
| Country | Link |
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| CN (1) | CN202222239U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104807967A (en) * | 2015-05-12 | 2015-07-29 | 北京电子科技职业学院 | Device and method for simulating emission reduction effect of vegetation on gas pollutants |
-
2011
- 2011-06-23 CN CN2011202153997U patent/CN202222239U/en not_active Expired - Lifetime
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104807967A (en) * | 2015-05-12 | 2015-07-29 | 北京电子科技职业学院 | Device and method for simulating emission reduction effect of vegetation on gas pollutants |
| CN104807967B (en) * | 2015-05-12 | 2016-08-24 | 北京电子科技职业学院 | A kind of vegetation is to gas pollutant emission reduction effect analog and method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C53 | Correction of patent for invention or patent application | ||
| CB03 | Change of inventor or designer information |
Inventor after: Gao Yike Inventor after: Mou Ningning Inventor after: Zhang Qixiang Inventor after: Cheng Tangren Inventor before: Gao Yike Inventor before: Zhang Qixiang Inventor before: Cheng Tangren |
|
| COR | Change of bibliographic data |
Free format text: CORRECT: INVENTOR; FROM: GAO YIKE ZHANG QIXIANG CHENG TANGREN TO: GAO YIKE MOU NINGNING ZHANG QIXIANG CHENG TANGREN |
|
| TR01 | Transfer of patent right |
Effective date of registration: 20170519 Address after: Temple town 223651 Suqian city in Jiangsu province Shuyang County on the north side of Zhaxinlu Patentee after: Jiangsu Subei Flower Co., Ltd. Address before: 100083 Haidian District Qinghua East Road, No. 35, Beijing Patentee before: Beijing Forestry University |
|
| TR01 | Transfer of patent right | ||
| CX01 | Expiry of patent term |
Granted publication date: 20120523 |
|
| CX01 | Expiry of patent term |