CN205884284U - Plant tissue culture system - Google Patents

Plant tissue culture system Download PDF

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Publication number
CN205884284U
CN205884284U CN201620796291.4U CN201620796291U CN205884284U CN 205884284 U CN205884284 U CN 205884284U CN 201620796291 U CN201620796291 U CN 201620796291U CN 205884284 U CN205884284 U CN 205884284U
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CN
China
Prior art keywords
filter
gas
pipeline
culture vessel
plant tissue
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Expired - Fee Related
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CN201620796291.4U
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Chinese (zh)
Inventor
肖玉兰
张宇
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Yangtze Delta Region Institute of Tsinghua University Zhejiang
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Yangtze Delta Region Institute of Tsinghua University Zhejiang
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Priority to CN201620796291.4U priority Critical patent/CN205884284U/en
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Publication of CN205884284U publication Critical patent/CN205884284U/en
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  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The utility model discloses a plant tissue culture system, including CO2 steel bottle, solenoid valve, first filter, second filter, gas circulation case, fan, CO2 concentration meter, air flow meter, large -scale culture vessel, gas -cooled pipe, air pump, the CO2 steel bottle is connected with the solenoid valve through gas pipeline, and it is simultaneously associative with CO2 concentration meter and first filter that the solenoid valve passes through the pipeline, and first filter is connected with the gas circulation case through the pipeline, and the gas circulation incasement is equipped with the fan, and it is associative with one side of large -scale culture vessel that CO2 concentration meter, air flow meter pass through the pipeline, it is associative with the opposite side and the gas circulation case of large -scale culture vessel that gas -cooled pipe, air pump, second filter pass through the pipeline. The utility model discloses can show the growth that promotes little plant, the seedling quality is showing and is improving, and cultivation period shortens, and reduction in production cost is favorable to the fast numerous scale of plant, batch production production.

Description

A kind of plant tissue culture system
Technical field
This utility model belongs to plant tissue culture technical field, is related to a kind of plant tissue culture system, more particularly, to a kind of large-scale The plant tissue culture system of culture vessel and forced ventilation.
Background technology
The target of micropropagation is to obtain that substantial amounts of heredity is identical, physiology is consistent, growth promoter is normally planted at short notice Strain.However, in traditional plant tissue culture micropropagation, the carbon source of plantlet growth depends on the sugar in culture medium, But sugar promotes the pollution of microorganism;For avoiding pollution it has to be cultivated using airtight small container, lead to plant physiology Disorder, seedling poor quality, the low outstanding problem of transplanting survival rate.And, small container complex operation, human resourcess consume big, machine Tool low degree is it is difficult to realize plant tissue culture factorial praluction numerous soon.
Plant sugar-free culture micropropagation is with co2Sugar in substitutive medium, as carbon source, controls handss using artificial environment Section, the growth for plantlet in container provides suitable illumination, temperature, humidity, co2The conditions such as concentration, nutrition, thus promote to plant The growth promoter of thing and Fast-propagation.Overcome pollution rate height in traditional tissue culture, growth and development of plants is bad, physiology and appearance is disorderly Disorderly, the problems such as deformity, growth promoter delay or are dead;It is remarkably improved seedling quality, shortens cultivation cycle, improve work and produce Rate, reduces production cost.Plant sugar-free cultivate micro-propagation technique one of the advantages is that due to culture medium not sugary, decrease dirt Dye occurrence probability is so that the maximization of culture vessel is possibly realized;Achieve conversion from test tube to workshop for the culture vessel, for planting The factorial praluction of Seedling provides guarantee.
However, the enforcement of plant sugar-free culture technique, need the technology of environmental Kuznets Curves and corresponding facility, in particular for Meet required illumination during plant culturing, the environmental condition such as carbon source, temperature, humidity;In view of this, nowadays in the urgent need to Designing a kind of new training method, to overcome the existing drawbacks described above with the presence of sugared training method, realizing plant sugar-free numerous soon Seedling factorial praluction.
Utility model content
Technical problem to be solved in the utility model is: provides a kind of plant tissue culture system it is ensured that plant was being cultivated Cheng Zhong, required illumination, carbon source, temperature, humidity are sufficiently met, particularly can be according to plant growing situation and light cooperation With adjusting air velocity, rate of ventilation and supplementary co2 gas.Achieve conversion from test tube to workshop for the culture vessel, be seedling Factorial praluction provide guarantee.
For solving above-mentioned technical problem, this utility model adopts the following technical scheme that
A kind of plant tissue culture system, described plant tissue culture system includes: co2Steel cylinder, electromagnetic valve, the first filter, second Filter, gas circulating box, fan, co2Densitometer, air flow meter, large-scale culture vessel, gas cooling pipe, air pump;
Described co2Steel cylinder is connected with electromagnetic valve by gas pipeline, electromagnetic valve pass through pipeline simultaneously with co2Densitometer and First filter is connected, and the first filter is connected with gas circulating box by pipeline, and gas circulating box is built with fan;co2 Densitometer, air flow meter are connected by the side of pipeline and large-scale culture vessel;Gas cooling pipe, air pump, the second filter pass through Pipeline is connected with the opposite side of large-scale culture vessel gentle body circulation case.
As a kind of preferred version of the present utility model, described large-scale culture vessel is made up of transparent material, in sealing shape, It is shaped as cuboid;Large-scale culture vessel includes mandatory air interchanger, mandatory air interchanger include the first filter, Tow filtrator, air pump.
As a kind of preferred version of the present utility model, in described large-scale culture vessel gas the top of a side equipped with Supplement co2The diffuser of gas, diffuser is uniformly thick with the aperture of needle-like, co2Gas can pass through co2Close on diffuser The aperture of cloth is even in large-scale culture vessel.
As a kind of preferred version of the present utility model, the external gas recycling duct of described culture vessel, and it is furnished with gas Body cooling tube is it is ensured that the temperature in container is consistent with outside container.
As a kind of preferred version of the present utility model, equipped with described air pump, the first filtration on described gas recycling duct Device, the second filter.
A kind of plant tissue culture system is it is characterised in that described plant tissue culture system includes: co2Storage container, electromagnetic valve, First filter, the second filter, gas circulating box, air mixing machine structure, co2Concentration detection apparatus, air flow meter, culture vessel, gas Body chiller, air pump;
Described co2Storage container is connected with electromagnetic valve by gas pipeline, electromagnetic valve pass through pipeline simultaneously with co2Concentration Detection means and the first filter are connected, and the first filter is connected with gas circulating box by pipeline, and gas circulating box is built-in There are air mixing machine structure, co2Concentration detection apparatus, air flow meter are connected by pipeline and culture vessel;Gas cooling device, air pump, Tow filtrator is connected by pipeline and culture vessel gentle body circulation case.
The beneficial effects of the utility model are: the utility model proposes plant tissue culture system, solve tradition tissue The problems such as growth and development of plants present in culture is bad, physiology and appearance is disorderly, lopsided, growth promoter delays or be dead, adopts Large-scale culture vessel and mandatory air exchange system, can remarkably promote the growth of plantlet, and seedling quality significantly improves, cultivation cycle Shorten, reduce production cost, be conducive to scale, the factorial praluction of plant fast propagation.
Brief description
Fig. 1 is the composition schematic diagram of this utility model system.
Specific embodiment
Describe preferred embodiment of the present utility model below in conjunction with the accompanying drawings in detail.
Embodiment one
Refer to Fig. 1, this utility model discloses a kind of plant tissue culture system, comprising: co2Steel cylinder 1, electromagnetic valve 2, first Filter 3, gas circulating box 4, fan 5, co2Densitometer 6, air flow meter 7, large-scale culture vessel 8, gas cooling pipe 9, air pump 10th, the second filter 11.All parts are all to be connected with large-scale culture vessel by pipeline.
co2Steel cylinder 1 is connected with electromagnetic valve 2 by gas pipeline, electromagnetic valve 2 pass through pipeline simultaneously with co2Densitometer 6 He First filter 3 is connected, and the first filter 3 is connected with gas circulating box 4 by pipeline, and gas circulating box 4 is built with fan 5;co2Densitometer 6 and air flow meter 7 are connected by the side of pipeline and large-scale culture vessel 8.One in large-scale culture vessel 8 gas The top of individual side is equipped with supplementary co2The pipeline of gas, pipeline has many apertures, with sharp co2It is uniformly distributed;Gas cooling pipe 9th, air pump 10, the second filter 11 are connected with the opposite side gentle body circulation case 4 of large-scale culture vessel 8 by pipeline.Gas is cold But the effect of pipe 9 is the gas temperature being lowered in culture vessel, because in general, is cultivated due to due to illumination The outer high 1-2 degree of temperature container in container.
The method of operation of this utility model plant tissue culture system is: co2The co of high concentration in steel cylinder2Gas is by electromagnetic valve control System, enters gas circulating box through the first filter, through co after mixing with air2Densitometer, air flow meter control the large-scale culture of entrance Container for plant culturing or carries out photosynthesis, for the gas after plant absorption co2 through gas cooling pipe, air pump, the second filtration Device, after be sent back to gas circulating box and carry out new circulation again.Co2 concentration is by electromagnetic valve and co2Densitometer is connected to control, gas Flow velocity by air flow meter control.
Co2 concentration and air velocity, example can be adjusted according to the species of plant and growing state during plant culturing Co2 concentration as c4 plant is adjustable to 2000-2500ppm, c3 plant 1000-1500ppm.In plant culturing, the stream of gas Amount can be gradually increased with the prolongation of plantlet incubation time and constantly increase with photosynthetic enhancing in other words.
Embodiment two
A kind of plant tissue culture system is it is characterised in that described plant tissue culture system includes: co2Storage container, electromagnetic valve, First filter, the second filter, gas circulating box, air mixing machine structure, co2Concentration detection apparatus, air flow meter, culture vessel, gas Body chiller, air pump;
Described co2Storage container is connected with electromagnetic valve by gas pipeline, electromagnetic valve pass through pipeline simultaneously with co2Concentration Detection means and the first filter are connected, and the first filter is connected with gas circulating box by pipeline, and gas circulating box is built-in There are air mixing machine structure, co2Concentration detection apparatus, air flow meter are connected by pipeline and culture vessel;Gas cooling device, air pump, Tow filtrator is connected by pipeline and culture vessel gentle body circulation case.
In sum, the utility model proposes plant tissue culture system, solve plant present in traditional tissue culture Retarded growth, physiology and appearance is disorderly, deformity, the problems such as growth promoter delays or be dead, using large-scale culture vessel and strong Property air exchange system processed, can remarkably promote the growth of plantlet, seedling quality significantly improves, and cultivation cycle shortens, and reduce and produce into This, be conducive to scale, the factorial praluction of plant fast propagation.
Here description of the present utility model and application are illustrative, are not wishing to be limited in scope of the present utility model State in embodiment.The deformation of embodiments disclosed herein and change are possible, for the ordinary skill people of those this areas For member, the replacement of embodiment and equivalent various parts are known.It should be appreciated by the person skilled in the art that not taking off In the case of spirit or essential characteristics of the present utility model, this utility model can in other forms, structure, arrangement, ratio, And realized with other assemblies, material and part.In the case of without departing from this utility model scope and spirit, can be to this In disclosed embodiment carry out other deformation and change.

Claims (6)

1. a kind of plant tissue culture system is it is characterised in that described plant tissue culture system includes: co2Steel cylinder, electromagnetic valve, the first filtration Device, the second filter, gas circulating box, fan, co2Densitometer, air flow meter, large-scale culture vessel, gas cooling pipe, air pump;
Described co2Steel cylinder is connected with electromagnetic valve by gas pipeline, electromagnetic valve pass through pipeline simultaneously with co2Densitometer and first Filter is connected, and the first filter is connected with gas circulating box by pipeline, and gas circulating box is built with fan;co2Concentration Meter, air flow meter are connected by the side of pipeline and large-scale culture vessel;Gas cooling pipe, air pump, the second filter pass through pipeline It is connected with the opposite side of large-scale culture vessel gentle body circulation case.
2. plant tissue culture system according to claim 1 it is characterised in that:
Described large-scale culture vessel is made up of transparent material, and in sealing shape, it is shaped as cuboid;Large-scale culture vessel includes by force Property air interchanger processed, mandatory air interchanger includes the first filter, the second filter, air pump.
3. plant tissue culture system according to claim 1 it is characterised in that:
In described large-scale culture vessel, the top of a side is equipped with supplementary co2The diffuser of gas, diffuser is uniformly thick with The aperture of needle-like, co2Gas can pass through co2The aperture gathering on diffuser is even in large-scale culture vessel.
4. plant tissue culture system according to claim 1 it is characterised in that:
The external gas recycling duct of described culture vessel, and it is furnished with gas cooling pipe it is ensured that outside temperature in container and container Unanimously.
5. plant tissue culture system according to claim 4 it is characterised in that:
Equipped with described air pump, the first filter, the second filter on described gas recycling duct.
6. a kind of plant tissue culture system is it is characterised in that described plant tissue culture system includes: co2Storage container, electromagnetic valve, first Filter, the second filter, gas circulating box, air mixing machine structure, co2Concentration detection apparatus, air flow meter, culture vessel, gas are cold But device, air pump;
Described co2Storage container is connected with electromagnetic valve by gas pipeline, electromagnetic valve pass through pipeline simultaneously with co2Concentration Testing Device and the first filter are connected, and the first filter is connected with gas circulating box by pipeline, and gas circulating box is built with mixed Mechanism of qi structure, co2Concentration detection apparatus, air flow meter are connected by pipeline and culture vessel;Gas cooling device, air pump, the second mistake Filter is connected by pipeline and culture vessel gentle body circulation case.
CN201620796291.4U 2016-07-25 2016-07-25 Plant tissue culture system Expired - Fee Related CN205884284U (en)

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Application Number Priority Date Filing Date Title
CN201620796291.4U CN205884284U (en) 2016-07-25 2016-07-25 Plant tissue culture system

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110806775A (en) * 2019-12-05 2020-02-18 上海离草科技有限公司 Automatic control device for plant container seedling
WO2021017656A1 (en) * 2019-07-27 2021-02-04 万贤能 Plant cultivation environment adjustment system and method
CN113359600A (en) * 2021-06-30 2021-09-07 青岛建华食品机械制造有限公司 Corona case CO2Monitoring system
CN115529984A (en) * 2022-10-25 2022-12-30 陕西师范大学 Plant cultivation greenhouse CO 2 Enhanced construction experiment control system

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021017656A1 (en) * 2019-07-27 2021-02-04 万贤能 Plant cultivation environment adjustment system and method
CN110806775A (en) * 2019-12-05 2020-02-18 上海离草科技有限公司 Automatic control device for plant container seedling
CN113359600A (en) * 2021-06-30 2021-09-07 青岛建华食品机械制造有限公司 Corona case CO2Monitoring system
CN115529984A (en) * 2022-10-25 2022-12-30 陕西师范大学 Plant cultivation greenhouse CO 2 Enhanced construction experiment control system

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CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20170118

Termination date: 20170725

CF01 Termination of patent right due to non-payment of annual fee