CN202143383U - In-situ cultivation barrel for phytoplankton - Google Patents
In-situ cultivation barrel for phytoplankton Download PDFInfo
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- CN202143383U CN202143383U CN201120242490U CN201120242490U CN202143383U CN 202143383 U CN202143383 U CN 202143383U CN 201120242490 U CN201120242490 U CN 201120242490U CN 201120242490 U CN201120242490 U CN 201120242490U CN 202143383 U CN202143383 U CN 202143383U
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- cultivation
- staving
- mesh screen
- barrel
- bucket
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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Abstract
The utility model discloses an in-situ cultivation barrel for phytoplankton, comprising a cylindrical barrel body which is made of an organic glass material; openings are arranged on the wall of the barrel body; the openings which are arranged on the side wall of the barrel body are arranged in layers and each layer has two openings which are dead against each other; the openings of every two adjacent layers are crossly arranged; a mesh screen which is used for covering the openings and made of a Sefar mesh screen is arranged on the wall of the barrel body; and the mesh screen is arranged on an organic glass frame which tightly clings to the outer wall of the barrel body. Water flow exchange can be carried out on the periphery of the side wall of the cultivation barrel, so that the influences on the growth of the phytoplankton caused by the nutritive salt in the water and the flow velocity can be fully reflected; the cylindrical cultivation barrel is small in volume, convenient to carry and small in required algae during the cultivation, so that the difficulty of indoor algae cultivation work is lightened; and the cultivation barrel is small in diameter and large in speed and the top of the cultivation barrel is exposed above the water level, so that the cultivation barrel does not need to be completely lifted up for sampling, the sampling is convenient and the mesh screen bears less pressure and is not easy to break.
Description
Technical field
The utility model relates to culture apparatus, is specifically related to the good phytoplankton original position of a kind of culture effect and cultivates bucket.
Background technology
Phytoplankton original position cultivation at present is to utilize natural phytoplankton cluster; Remove the predation of zooplankton through manual operation after; The container of putting into sealing in position or the device of simulation original position cultivate; Its purpose is to confirm single growth characteristics in actual waters of planting algae or main phytoplankton cluster, is used to estimate the Primary Production potentiality of phytoplankton, the ecological data that the comparatively validate laboratory obtains.Phytoplankton original position culture apparatus need possess the characteristic of two aspects: 1) can support phytoplankton growth, avoid it in incubation, to become feeble and die because of installing discomfort; 2) guarantee that the phytoplankton habitat conditions approaches actual natural conditions.
Traditional original position training method mainly adopts transparent vial to carry out the cultivation of phytoplankton for hanging the bottle method, but because it cultivates the water flow exchange existing problems for closed container.At present, mostly the original position culture apparatus is cube transparent organic glass incubator, and the incubator volume is 50L; Sealing around the incubator, top drilling is used for sample circuit and adds the algae kind, and the bottom mesh screen covers; Can stop the turnover of colony Microcystis aeruginosa and even individual cells; All be applied in the class water body Microcystis aeruginosa growth in situ The Characteristics of lake, the more traditional extension bottle of original position culture apparatus is cultivated has obvious improvement, but also has tangible weak point:
1. incubator only can carry out water flow exchange in the bottom, and is bigger for horizontal flow velocity, and vertically the less water body of disturbance is difficult to realize effective water flow exchange, thereby can not embody in the water body nutritive salt and flow velocity for the influence of phytoplankton growth;
2. the incubator volume is bigger, need add more algae kind to reach rational initial concentration for the original position cultivation of purebred algae, and this just cultivates indoor algae kind and has strengthened difficulty;
3. sampling is inconvenient, need incubator is taken out from water, and the bottom is a mesh screen that culture fluid can spill unavoidably;
4. install the process of in water, transferring or from water, taking out, the bottom mesh screen bears big pressure, breaks easily.
5. device is cube structure, and volume is big, and it is bigger that the process of transferring behind the top seal reaches the suffered buoyancy in top all around, is increased in and transfers difficulty in the water body.
The utility model content
The utility model technical problem to be solved is; Be difficult to realize the problem of effective water flow exchange at water body to existing incubator; Provide the high original position of a kind of water flow exchange efficient to cultivate bucket, it is big that this original position cultivation bucket also has the little degree of depth of volume, and the cultivation difficulty is little; Sampling is convenient, and advantage fragile, not easy to carry when using.
In order to solve the problems of the technologies described above, the utility model provides a kind of phytoplankton original position to cultivate bucket, it is characterized in that comprise staving, barrel body wall is provided with perforate, and the mesh screen that covers said perforate also is installed on the barrel body wall.
Said staving is cylindrical, and this columniform diameter is less, and length is greater than diameter, and the perforate layering on the staving sidewall is provided with, every layer have two over against perforate, the perforate of adjacent two layers all cross is crisscross arranged.
Said mesh screen all is installed on the organic glass frame, and this organic glass frame closely sticks on said staving inwall or the outer wall.
Said staving top is provided with opening, and the top cover that cooperates with open top top seal is installed at its top.
Said staving top sidewall has aperture, and the rope that is used to hang said staving is installed through this aperture.
Said staving and top cover all are to be processed by pmma material.
Said mesh screen adopts the Sefar mesh screen to process.
Compare prior art, the utlity model has following beneficial effect:
1. cultivate bucket sidewall four sides and all can carry out water flow exchange, can reflect fully that nutritive salt and flow velocity are for the influence of phytoplankton growth in the water body.
2. columniform cultivation staving is long-pending little, easy to carry, and required algae kind is few during cultivation, alleviates the difficulty of indoor algae kind training.
3. it is big to cultivate the little degree of depth of bucket diameter, cultivates the bucket top and is exposed to more than the water surface, and therefore sampling need not be mentioned cultivating bucket, and sampling is convenient.
4. cultivating bucket is the side perforate, and it is little that the upper and lower handling mesh screen that installs in the incubation bears pressure, is difficult for breaking long service life.
5. device is for small diameter cylinders shape structure, and the top is open, and it is little to transfer the suffered buoyancy in process bottom, and it is little to transfer difficulty.
6. cultivate bucket adopt light transmission preferably pmma material and the high Sefar mesh screen of the even precision of perforate process, can guarantee that phytoplankton accepts sufficient illumination and non-leakage.
Description of drawings
Fig. 1 is the structural representation of the utility model;
Fig. 2 is for cultivating phosphate penetration curve sketch map in the bucket.
Embodiment
Below in conjunction with specific embodiment the utility model is done further explain.
As shown in Figure 1, a kind of phytoplankton original position is cultivated bucket, comprises the columniform staving of being processed by pmma material 3; Staving 3 walls are provided with four perforates, and perforate layering on these staving 3 sidewalls is provided with, every layer have two over against perforate; The perforate of adjacent two layers all cross is crisscross arranged, and four mesh screens 5 that adopt Sefar mesh screen (Switzerland's mesh screen) to process that cover this perforate also are installed on staving 3 walls, and mesh screen 5 all is installed on the organic glass frame 4; This organic glass frame 4 closely sticks on staving 3 outer walls; Staving 3 tops are provided with opening, and the top cover 1 that cooperates with open top top seal is installed at its top, and wherein top cover 1 is processed by pmma material; Staving 3 top sidewalls have aperture, and the rope 2 that is used to hang staving 3 is installed through this aperture.
When carrying out the cultivation of phytoplankton original position, the cultivation bucket of this utility model places water, surfaces in top, through rope 2 this device is hung.Water flow exchange all can be carried out in this cultivation bucket sidewall four sides, and water flow exchange efficient is high, can reflect the influence of the flow velocity of nutritive salt and water in the water body for phytoplankton growth fully, for the growth of phytoplankton provides a growing environment that is similar to original position.
The cylindrical cultivation barrel cultivation staving that the little degree of depth of diameter is big is long-pending little, easy to carry, and required algae kind is few, alleviates the difficulty of indoor algae kind training, cultivates a bucket top and is exposed to more than the water surface, so take a sample and need not mention cultivating bucket, and sampling conveniently.
Cultivating bucket is the side perforate, and it is little that the upper and lower handling mesh screen that installs in the incubation bears pressure, is difficult for breaking.
Cylindrical cultivation bucket be a small diameter cylinders shape structure, and the top is open, and floor space is little, and it is little to transfer bottom the process suffered buoyancy, and it is little to transfer difficulty.
Cultivating bucket adopts light transmission pmma material and mesh screen 5 adopts the high Sefar mesh screen of the even precision of perforate to process preferably; The Sefar mesh screen is the lightweight mesh screen; Its light transmission is good, mesh is even and precision is high; Can stop the turnover of colony Microcystis aeruginosa and even individual cells, guarantee that simultaneously phytoplankton accepts sufficient illumination.
Need carry out attribute testing for the effect of verifying this device, attribute testing comprises the mensuration of water flow exchange speed trial, frustule leak test and light reduction rate altogether, and concrete operations and result are following:
1. water flow exchange speed: the phosphate solution of certain volume and concentration is packed in the culture apparatus, will cultivate bucket again and flood in the water-butt that certain volume distilled water is housed; Adopt magnetic stirring apparatus constantly to stir the water in the water-butt therebetween; Cultivate the inside and outside phosphate concn of bucket bucket and survey at regular intervals once, being more or less the same up to concentration just stops, and makes penetration curve (as shown in Figure 2).Formula Y=Xe is followed in the phosphate infiltration
BT, wherein X is an initial concentration, T is the time, thus, calculates half life period and water flow exchange speed.Result of the test is seen Fig. 2.Through calculating, cultivating bucket water flow exchange speed is 0.65mlcm
-2H
-1, half only needs 1.92h nutritive salt exchange, this shows that this cultivations barrel can carry out water flow exchange effectively, is convenient to reflect fully the influence of the flow velocity of nutritive salt and water in the water body for phytoplankton growth.
2. frustule seepage situation: 6 kinds of purebred algaes (Hubei little ring algae, beautiful star bar algae, wawter bloom anabena, microcystic aeruginosa, avette latent algae, empty ball algae) are mixed by same ratio and be made into to pour into behind the high cell concentration cultivate in the bucket; To cultivate bucket again and flood in the water-butt that certain volume distilled water is housed, and adopt magnetic stirring apparatus to stir the water in the water-butt continuously.Long run test is carried out microscopy after in water-butt, taking a sample after 24 hours and precipitating 48 hours.The result shows that under the condition of continuous stirring (average water velocity is 0.2m/s), frustule seepage phenomenon is all not obvious.
3. light reduction rate: record with illuminometer on the roof of spaciousness and to cultivate the inside and outside light intensity of bucket, calculate the reduction rate of light.Measuring the result, to cultivate the light transmission of bucket fairly good, and light decay rate is merely about 13%.
Embodiment: under suitable weather condition (March), test adopts five cultivation buckets and five kinds of purebred algaes to carry out in Pinghu, Gaoyang, Peng Xi river, and five kinds of algaes are respectively microcystic aeruginosa, beautiful star bar algae, empty ball algae, the little ring of wawter bloom anabena and Hubei algae.Water sample is that superficial water filters back entering cultivation bucket; Each is cultivated and adds the purebred algae liquid of certain volume in the bucket; Cultivate and carried out altogether two days, utilize and cultivate the chlorophyllous change calculating in front and back phytoplankton growth speed, the growth rate of each algae is respectively: wawter bloom anabena 0.188d
-1, beautiful star bar algae 1.429d
-1, microcystic aeruginosa 0.417d
-1, empty ball algae 0.877d
-1, Hubei little ring algae 0.587d
-1The growth of five kinds of algaes all clearly in two days; And The above results has more excellent contrast property with phytoplankton composition, successional rate result in the actual water body, explains that the utility model lucite cultivation bucket can provide a growing environment that is similar to original position for the growth of phytoplankton.
The foregoing description of the utility model only is to give an example for what explanation the utility model was done, and is not to be the qualification to the embodiment of the utility model.For the those of ordinary skill in affiliated field, on the basis of above-mentioned explanation, can also make other multi-form variation and changes.Here can't give exhaustive to all embodiments.Conspicuous variation that every technical scheme that belongs to the utility model is amplified out or change still are in the row of the protection domain of the utility model.
Claims (7)
1. a phytoplankton original position is cultivated bucket, it is characterized in that comprise staving (3), staving (3) wall is provided with perforate, and the mesh screen (5) that covers said perforate also is installed on staving (3) wall.
2. cultivation according to claim 1 bucket is characterized in that said staving (3) is cylindrical, and the perforate layering on this staving (3) sidewall is provided with, every layer have two over against perforate, the perforate of adjacent two layers all cross is crisscross arranged.
3. cultivation bucket according to claim 1 is characterized in that said mesh screen (5) all is installed on the organic glass frame (4), and this organic glass frame (4) closely sticks on said staving (3) inwall or the outer wall.
4. according to claim 1,2 or 3 described cultivations bucket, it is characterized in that said staving (3) top is provided with opening, the top cover (1) that cooperates with open top top seal is installed at its top.
5. cultivation bucket according to claim 4 is characterized in that said staving (3) top sidewall has aperture, installs through this aperture to be used to hang the rope (2) of said staving (3).
6. cultivation bucket according to claim 4 is characterized in that said staving (3) and top cover (1) all are to be processed by pmma material.
7. according to claim 1 or 3 described cultivation buckets, it is characterized in that said mesh screen (5) adopts the Sefar mesh screen to process.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201120242490U CN202143383U (en) | 2011-07-11 | 2011-07-11 | In-situ cultivation barrel for phytoplankton |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201120242490U CN202143383U (en) | 2011-07-11 | 2011-07-11 | In-situ cultivation barrel for phytoplankton |
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| Publication Number | Publication Date |
|---|---|
| CN202143383U true CN202143383U (en) | 2012-02-15 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201120242490U Expired - Fee Related CN202143383U (en) | 2011-07-11 | 2011-07-11 | In-situ cultivation barrel for phytoplankton |
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| Country | Link |
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| CN (1) | CN202143383U (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104498329A (en) * | 2014-12-22 | 2015-04-08 | 浙江海洋学院 | Micro-algae generator device for open shellfish aquaculture area |
| CN104488788A (en) * | 2014-12-22 | 2015-04-08 | 浙江海洋学院 | Method for increasing supply quantities of baits for natural shellfishes in aquaculture areas on open seas |
| CN104498322A (en) * | 2014-11-18 | 2015-04-08 | 中国海洋大学 | In-situ high flux culture apparatus for sea microbe |
| CN106485312A (en) * | 2016-10-17 | 2017-03-08 | 大连海洋大学 | A kind of tangleweed trip Spore adhesion is observed and counting assembly |
| CN115161202A (en) * | 2022-07-12 | 2022-10-11 | 中国科学院水生生物研究所 | In-situ culture method and culture system for plankton |
-
2011
- 2011-07-11 CN CN201120242490U patent/CN202143383U/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104498322A (en) * | 2014-11-18 | 2015-04-08 | 中国海洋大学 | In-situ high flux culture apparatus for sea microbe |
| CN104498329A (en) * | 2014-12-22 | 2015-04-08 | 浙江海洋学院 | Micro-algae generator device for open shellfish aquaculture area |
| CN104488788A (en) * | 2014-12-22 | 2015-04-08 | 浙江海洋学院 | Method for increasing supply quantities of baits for natural shellfishes in aquaculture areas on open seas |
| CN106485312A (en) * | 2016-10-17 | 2017-03-08 | 大连海洋大学 | A kind of tangleweed trip Spore adhesion is observed and counting assembly |
| CN106485312B (en) * | 2016-10-17 | 2018-09-04 | 大连海洋大学 | A kind of trip Spore adhesion observation of tangleweed and counting device |
| CN115161202A (en) * | 2022-07-12 | 2022-10-11 | 中国科学院水生生物研究所 | In-situ culture method and culture system for plankton |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120215 Termination date: 20160711 |
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| CF01 | Termination of patent right due to non-payment of annual fee |