CN103852453A - Fluorescence image-based alga chlorophyll a concentration detection device - Google Patents
Fluorescence image-based alga chlorophyll a concentration detection device Download PDFInfo
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- CN103852453A CN103852453A CN201210506853.3A CN201210506853A CN103852453A CN 103852453 A CN103852453 A CN 103852453A CN 201210506853 A CN201210506853 A CN 201210506853A CN 103852453 A CN103852453 A CN 103852453A
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Abstract
The invention provides a fluorescence image-based alga chlorophyll a concentration detection device, and aims at providing a non-contact movable alga chlorophyll a concentration detection device capable of measuring multiple points. Relatively stable fluorescence 14 can be excited through modulated purple light generated by a purple light laser driving circuit 1 and a laser device 2. Fluorescent images collected by a fluorescent light filter 8, a lens group 9 and a CCD (Charge Coupled Device) detector 10 are delivered into a computer 11, the fluorescent light spot grey value detected by the computer 11 is divided by a reference signal collected by a light detector 5, an ultraviolet light detector amplifying circuit 6 and an A/D (Analog-Digital) conversion circuit 7, in the computer 11 through adopting the differential principle to obtain a value which serves as the alga chlorophyll a concentration value, so that the relatively precise alga chlorophyll a concentration value can be obtained. The fluorescence image-based alga chlorophyll a concentration detection device is designed as a device capable of remotely detecting the alga chlorophyll a in a non-contact manner, and is applicable to polling of unmanned aircrafts and ships in various lakes and seas.
Description
Affiliated technical field:
The present invention is a kind of design of device of remote non-contact detecting algae chlorophyll a.The design detects for unmanned vehicle and the boat-carrying of various lakes and ocean.
Background technology
Along with the economic fast development of China, the continuous increase of population, environmental pollution constantly increases the weight of, and increasing people start to pay close attention to the detection of lake and marine environment.And the taking place frequently of " red tide " phenomenon in the growth and ocean of lake algae excessive in recent years, causing the mass mortality of fish, a lot of aquatic organisms are all deeply hurt, and the large-area lake resource of China and ocean resources are destroyed.
The method of measuring at present marine alga concentration mainly contains following several: cell counting, spectrophotometric method, fluorescence method, satellite remote sensing detection method and airborne remote sensing method.Cell counting is mainly the number of utilizing microscopic examination cell, and spectrophotometric method is that the sample collecting is detected in laboratory, and trace routine is cumbersome, is unfavorable for continuous coverage.Although utilizing Remote Sensing Principles to carry out monitoring method with satellite or aircraft can realize large area and measure the mechanism of sea chlorophyll remote sensing, be that the phytoplankton concentration based on different has different radiation spectrum characteristics to detect.The shortcoming of this method be the large weight ratio of equipment volume heavier and when being subject to aqueous vapor and Influence of cloud remote monitoring measuring accuracy very poor.It is mainly on-line measurement that fluorescence method is measured, it is different from measuring method and principle although the method adopts fluorescent method, be automatic collected specimens and in sample chamber, utilize photodiode or photomultiplier, by the method that detects fluorescent intensity, algae chlorophyll-a concentration is detected.So an equipment can only be monitored a point, measure and certainly do not accomplish for large area.
Summary of the invention
The object of the invention is, overcome the shortcoming of prior art, provide a kind of highly sensitive, simple in structure, the gray-scale value that irradiates the fluoroscopic image of algae chlorophyll a generation by detecting purple light detects algae chlorophyll-a concentration, provide a kind of in lake or coastal waters can measure the noncontact packaged type algae chlorophyll-a concentration pick-up unit of multiple spot, accurately measure algae chlorophyll-a concentration for unmanned vehicle and boat-carrying gordian technique be provided.
The object of the invention is to be realized by following technical scheme:
Algae chlorophyll-a concentration pick-up unit based on fluoroscopic image, it comprises purple light drive circuit for laser 1, semiconductor purple light laser instrument 2, prism 3, Violet colour filter 4, purple light detector 5, purple light detector amplifying circuit 6, A/D change-over circuit 7, fluorescent optical filter 8, lens group 9, ccd detector 10, computing machine 11, laser module fixed mount 12, laser instrument and CCD module fixing rack.Purple light drive circuit for laser 1 drives semiconductor ultraviolet laser 2, the purple light of transmitting is through prism 3, by prism 3, light beam is divided into two-beam, the anti-utilizing emitted light of the other light beam of light beam transmission, reflection arrives purple light detector 5, the weak signal of exporting from photo-detector 5 is amplified by purple light detector amplifying circuit 6, then enters A/D change-over circuit 7, simulating signal is converted to digital signal and enter into computing machine 11 as with reference to signal.Inciding the other through Beam that the light of prism 3 is divided into is irradiated on testee and will be produced fluorescence by Violet colour filter 4, this wavelength of fluorescence, the fluorescence producing enters into lens group 9 by fluorescent optical filter 8, send into ccd detector 10 from the light signal of camera lens output, then light signal is converted to electric signal enters into computing machine 11 and shows fluorescence light spot image, the reference signal that we take differential type principle that the fluorescence hot spot gray-scale value that detects and photo-detector 5 are collected for fear of the measuring error causing due to the fluctuation of light source is divided by the value that obtains on computers as algae chlorophyll-a concentration.
Beneficial effect of the present invention: owing to reflecting algae chlorophyll-a concentration by the gray-scale value of fluorescence hot spot, so can realize in lake or coastal waters can measure multiple spot noncontact packaged type algae chlorophyll-a concentration detect.
Fig. 1 is the structural representation of the detection algae chlorophyll a based on fluoroscopic image.
Fig. 2 is purple light drive circuit for laser 1 circuit theory diagrams in Fig. 1.
Fig. 3 is purple light detector amplifying circuit 6 circuit theory diagrams in Fig. 1.
Fig. 4 is A/D change-over circuit 7 circuit theory diagrams in Fig. 1.
In Fig. 1: 1 purple light drive circuit for laser, 2 semiconductor ultraviolet lasers, 3 prisms, 4 Violet colour filters, 5 purple light detectors, 6 purple light detector amplifying circuits, 7A/D change-over circuit, 8 fluorescent optical filters, 9 lens group, 10CCD detector, 11 computing machines, 12 laser module fixed mounts, 13 laser instruments and CCD module fixing rack, the fluorescence hot spot under 14 purple light excitations.
The invention will be further described to utilize drawings and Examples below.
Purple light drive circuit for laser 1 drives semiconductor purple light laser instrument 2, produce the purple light modulated of 1000 hertz, adopting the object of light modulated is that CCD system can gather stable fluorescence (while using the chlorophyll a without the light signal excitation algae of light modulated, fluorescent intensity weakens in time).The purple light modulated of laser instrument transmitting is through prism 3, light beam is divided into two-beam by prism 3, the anti-utilizing emitted light of the other light beam of light beam transmission, reflection arrives purple light detector 5, the weak signal of exporting from photo-detector 5 is amplified by purple light detector amplifying circuit 6, then enter A/D change-over circuit 7, simulating signal is converted to digital signal and enter into computing machine 11 as with reference to signal, incide the other through Beam that the light of prism 3 is divided into, be irradiated on testee and will produce fluorescence by purple light optical filter 4, the object of purple light optical filter 4 is the purple lights that obtain 435 nano wave lengths.The fluorescence of 685 nano wave lengths that produce enters into lens group 9 by fluorescent optical filter 8, the effect of fluorescent optical filter 8 is the light of removing except the fluorescence of 685 nano wave lengths, the effect of lens group 9 is that fluoroscopic image is focused on ccd detector 10, by CCD, light signal being converted to electric signal then enters into computing machine 11 and shows fluorescence light spot image, the reference signal that we take differential type principle that the fluorescence hot spot gray-scale value that detects and photo-detector 5 are collected for fear of the measuring error causing due to the fluctuation of light source is divided by the value that obtains on computers as algae chlorophyll-a concentration value.
Claims (4)
1. the algae chlorophyll-a concentration pick-up unit based on fluoroscopic image comprises: purple light laser instrument sends purple light and excites algae chlorophyll a to produce fluorescence hot spot, gather fluoroscopic image by ccd detector, detect the concentration of algae chlorophyll a according to the gray-scale value of fluoroscopic image, its feature: purple light drive circuit for laser 1, semiconductor purple light laser instrument 2, prism 3, Violet colour filter 4, purple light detector 5, purple light detector amplifying circuit 6, A/D change-over circuit 7, fluorescent optical filter 8, lens group 9, ccd detector 10, computing machine 11, laser module fixed mount 12.
2. a kind of algae chlorophyll-a concentration pick-up unit based on fluoroscopic image according to claim 1, is characterized in that: purple light drive circuit for laser 1 drives semiconductor purple light laser instrument 2 to produce the laser that frequency is wavelength 435 nanometers of 1000 hertz.
3. according to the algae chlorophyll-a concentration pick-up unit based on fluoroscopic image of claim 1 or 2,
It is characterized in that: the reference signal of taking differential type principle that the fluorescence hot spot gray-scale value that detects and photo-detector 5 are collected is divided by the value that obtains on computers as algae chlorophyll-a concentration value.
4. according to the algae chlorophyll-a concentration pick-up unit based on fluoroscopic image of claim 1 or 2,
It is characterized in that: Violet colour filter 4 adopts and sees through optical maser wavelength is the interference filter of 435 nanometers,
Fluorescent optical filter 8 adopts and sees through optical maser wavelength is the interference filter of 685 nanometers.
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| CN201210506853.3A CN103852453A (en) | 2012-12-03 | 2012-12-03 | Fluorescence image-based alga chlorophyll a concentration detection device |
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Cited By (3)
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| CN105424669A (en) * | 2015-12-21 | 2016-03-23 | 江南大学 | On-line detection device of cyanobacteria density in water body |
| CN105527260A (en) * | 2015-12-21 | 2016-04-27 | 江南大学 | Online detection device of concentration of blue-green algae in water body |
| CN108414477A (en) * | 2018-01-12 | 2018-08-17 | 山东省科学院海洋仪器仪表研究所 | Seawater chlorophyll a, phycocyanin and phycoerythrin parameter measuring apparatus and method |
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