CN1986567A - 日本血吸虫雌虫性别特异基因、编码蛋白及其应用 - Google Patents
日本血吸虫雌虫性别特异基因、编码蛋白及其应用 Download PDFInfo
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Abstract
本发明公开了日本血吸虫(Schistosoma japonicum)雌性成虫特异蛋白及其基因序列,还公开了与该蛋白特异结合的抗体,以及把雌性成虫特异蛋白作为免疫原制备所得的日本血吸虫病防治疫苗,此外,本发明还公开了日本血吸虫雌性成虫特异蛋白在制备预防和治疗日本血吸虫病的药物中的应用。运用本发明的蛋白,可抑制血吸虫雌性成虫的产卵,从而治疗血吸虫病、防止血吸虫的扩散。
Description
技术领域
本发明涉及生物基因领域,尤其涉及日本血吸虫雌性成虫特异蛋白质的基因核酸序列、以及特异蛋白质的氨基酸序列;此外,本发明还涉及日本血吸虫雌性成虫特异基因、蛋白的应用。
背景技术
血吸虫病由血吸虫的感染引起,在世界上有76个国家,受感染者约1.5亿。致病的血吸虫有3种,既日本血吸虫(Schistosoma japonicum),曼氏血吸虫(S.mansoni)和埃及血吸虫(S.haematobium)。在我国流行的是日本血吸虫。血吸虫病的流行给人民身体健康和经济发展都造成巨大的损失,因此,防治血吸虫病、开发研制治疗血吸虫病和抗血吸虫的药物具有巨大的社会和经济效益。
研制新的治疗血吸虫病和抗血吸虫的药物,首先必须找到合适的药物作用靶点。血吸虫的虫卵是造成血吸虫病的最主要原因,肉芽肿就是由虫卵造成的。此外,虫卵排出体外进入外界环境,污染环境后会制造更多的感染。所以,治疗血吸虫病、防止血吸虫的扩散,一个关键的措施是抑制血吸虫雌虫成虫的产卵。这可以通过干扰雌虫性成熟,干扰雌、雄虫的配对,或者抑制产卵相关的生理途径来实现。而雌虫特异表达的蛋白质,很可能与雌虫性成熟、雌雄虫的配对,或者产卵密切相关。通过大规模的基因表达谱学研究,发现了日本血吸虫雌性成虫的特异蛋白质,这些蛋白质,都是可能的药物作用靶点和候选疫苗。
在本发明之前,还没有出现涉及本发明的日本血吸虫雌性成虫特异蛋白的公开报道。
发明内容
本发明要解决的技术问题之一是提供一类日本血吸虫雌性成虫特异蛋白,及其基因的核酸序列。
本发明要解决的技术问题之二是提供一类日本血吸虫病防治疫苗,该疫苗以日本血吸虫雌性成虫特异蛋白为免疫原。
本发明要解决的技术问题之三是提供一类与日本血吸虫雌性成虫特异蛋白结合的抗体。
本发明要解决的技术问题之四是提供日本血吸虫雌性成虫特异蛋白在制备预防和治疗日本血吸虫病的药物中的应用。
为了解决上述技术问题,本发明通过如下技术方案实现:
在本发明的一个方面,提供了一类日本血吸虫雌性成虫特异蛋白,包括:
(a)具有SEQ ID No.25~SEQ ID No.48所示氨基酸序列的蛋白;
(b)由(a)的蛋白发生一个或几个氨基酸置换、缺失或***而形成的具有(a)蛋白相同活性的蛋白。
较佳地,所述蛋白是具有SEQID No.25~SEQ ID No.48氨基酸序列的蛋白。
在本发明的另一方面,提供了一类编码日本血吸虫雌性成虫特异蛋白的基因,包括:
(a)SEQ ID No.1~SEQ ID No.24所示的核苷酸序列;
(b)SEQ ID No.1~SEQ ID No.24所示的核苷酸序列中每条序列的互补序列;
(c)与SEQ ID No.1~SEQ ID No.24所示的核苷酸序列中每条序列有至少70%同源性、且编码相同功能蛋白质的核苷酸序列。
较佳地,所述基因具有SEQ ID No.1~SEQ ID No.24所示的核苷酸序列。
在本发明的另一方面,还提供了一类日本血吸虫病防治疫苗,该疫苗以日本血吸虫雌性成虫特异蛋白为免疫原。
在本发明的另一方面,还提供了一类与日本血吸虫雌性成虫特异蛋白结合的抗体。
此外,本发明还提供了日本血吸虫雌性成虫特异蛋白在制备预防和治疗日本血吸虫病的药物中的应用。
在本发明中,术语“日本血吸虫雌性成虫特异蛋白”指具有日本血吸虫雌性成虫特异蛋白活性的SEQ ID No.25~SEQ ID No.48所示氨基酸序列的蛋白质。该术语还包括具有与日本血吸虫雌性成虫特异蛋白质相同功能的、SEQ ID No.25~SEQ IDNo.48序列的变异形式。这些变异形式包括(但并不限于):若干个(通常为1-50个,较佳地1-30个,更佳地1-20个,最佳地1-10个)氨基酸的缺失、***和/或取代,以及在C末端和/或N末端添加一个或数个(通常为20个以内,较佳地为10个以内,更佳地为5个以内)氨基酸。例如,在本领域中,用性能相近或相似的氨基酸进行取代时,通常不会改变蛋白质的功能。又比如,在C末端和/或N末端添加一个或数个氨基酸通常也不会改变蛋白质的功能。该术语还包括日本血吸虫雌性成虫特异蛋白的活性片段和活性衍生物。
在本发明中,术语“编码日本血吸虫雌性成虫特异蛋白的基因序列”指编码具有日本血吸虫雌性成虫特异蛋白活性的核苷酸序列,如SEQ ID No.1~SEQ ID No.24所示的核苷酸序列及其简并序列。该简并序列是指,位于SEQ ID No.1~SEQ ID No.24序列中,有一个或多个密码子被编码相同氨基酸的简并密码子所取代后而产生的序列。由于密码子的简并性,所以与SEQ ID No.1~SEQ ID No.24所示核苷酸序列的同源性低至约70%的简并序列也能编码出SEQ ID No.25~SEQ ID No.48所示的氨基酸序列。
在本发明中,可以使用一系列本领域已知的方法来制备针对日本血吸虫雌性成虫特异蛋白的抗体。例如,将提纯的日本血吸虫雌性成虫特异蛋白产物或它的抗原片段注射入动物体内以产生多克隆抗体。同样,表达日本血吸虫雌性成虫特异蛋白或它的抗原片段的细胞也可以用来对动物致免疫而产生抗体。本发明制备的抗体也可以是单克隆抗体,这些单克隆抗体可用杂交瘤技术制备。
应用本发明的日本血吸虫雌性成虫特异蛋白,可抑制血吸虫雌性成虫的产卵,从而治疗血吸虫病、防止血吸虫的扩散,该类蛋白在血吸虫病防治和治疗中有着重要的作用。
附图说明
图1是用RT-PCR实验验证日本血吸虫成虫特异表达基因的图;
图2是图1的数据分析图。
具体实施方式
下面结合附图和实施例对本发明作进一步详细的说明。
以下实施例仅用于说明本发明而不用于限制本发明的范围。实施例中未注明具体条件的实验方法,通常按照常规条件,例如Sambrook等人,分子克隆:实验室手册(New York:Cold Spring Harbor Laboratory Press,1989)中所述的条件,或按照制造厂商所建议的条件。
实施例1
日本血吸虫雌性成虫特异蛋白的生物信息学分析。
1.日本血吸虫雌性成虫特异蛋白的筛选:利用表达序列标签(EST)的数据,分析特异表达的蛋白。雌虫相对于雄虫特异表达的基因:1)其差异显著性必须满足p<0.05,这个显著性分析用网上专门的软件工具计算获得;2)每个基因,其EST数量必须大于等于10条;3)雌虫的EST数量必须是雄虫数量的3倍或3倍以上。经过这3个标准的筛选,得到了雌性成虫特异表达基因。
2.同源性和相似性分析:日本血吸虫雌性成虫特异蛋白序列对GenBank(http://www.ncbi.nlm.nih.gov/)的非冗余数据库(Non-redundant,NR)做BLASTP比对;日本血吸虫雌性成虫特异蛋白序列对Swissprot数据库做BLASTP同源性比对。根据比对结果得到的与已知其他物种或已知的血吸虫蛋白的序列相似性,做出日本血吸虫雌性成虫特异蛋白的功能注释。
实施例2
RT-PCR实验验证日本血吸虫雌性成虫特异蛋白的表达情况。
1.样品准备
准备日本血吸虫的成年雌虫和雄虫,以抽提其RNA。
2.总RNA抽提试剂盒
抽提RNA试剂采用TRIzol reagent(GIBCO/BRL),该试剂基于酸性酚一步抽提法生产。用于抽提RNA所用器皿和水均要进行无RNA酶处理,以保证实验中无RNA酶环境。
3.RNA抽提步骤
将碾杵和匀浆器等器皿在200℃干烤4h,去除RNA酶,冷却;加入液氮中预冷,将组织从液氮中迅速取出,碾成粉末;用刮匙将组织放入预先加入TRIzol试剂匀浆器中,匀浆数分钟;将匀浆后液体转入无RNA酶离管中,加入氯仿后,4℃离分层;将上层水相转入一无RNA酶离管中,加入氯仿后,4℃离分层;将上层水相转入一无RNA酶离管中,加异丙醇,4℃离沉淀RNA;用75%乙醇洗涤沉淀2次;用无RNA酶去离子水溶解沉淀。抽提RNA质量鉴定:紫外分光光度计测定260/20比值(比值均在1.7~2.0);并在MOPS甲醛变性胶中观察有无降解。
4.RT-PCR反应
用半定量反转录多聚酶链式反应(RT-PCR)来验证日本血吸虫雌性成虫中一些基因的表达丰度差异。采用不同的循环数,分别是21、24、27和30个反应循环。每个PCR重复3次。实验结果见图1和图2所示。
序 列 表
<110>上海人类基因组研究中心
<120>日本血吸虫雌虫性别特异基因、编码蛋白及其应用
<130>NP-10266
<160>48
<170>PatentIn version3.3
<210>1
<211>1442
<212>DNA
<213>日本血吸虫(Schistosoma japonicum)
<400>1
gttttgcgga gcgttgttaa ttagtgatgc gtgaatgtat cagtcttcat gttggtcagg 60
ccggagccca aatgggcgac tcttgttggg agctctactg ccttgagcat ggtatacaac 120
ctgatggaaa ggttcctagc gactctacta taaaaaatga caacgattct ttcagtacat 180
tttttagcga gaccagctat ggccgttacg tcccacgcgc tgtgttcgtc gacttagaac 240
cgtcggttat tgacagactg cgaacagggg cttataagca gttattccat cctgaacaaa 300
tgataagtgg aaaagaagat gctgccaata attacgctcg cggtcactat actgttggta 360
aggagttggt tgatgaagtc cttgaccgca tacgcaagtt gtctgaaaga tgcacagggc 420
ttcagggatt cttggtattt cattcatttg gcggtggaac aggatcagga ttcacgtctt 480
tgctgatgga acgcctctcg gttgatttcg gtaaaaaagc caagttagag tttgctgttt 540
acccagcacc tcatatctcg acagctgttg ttgaacccta taactccatt ctaaccacgc 600
atacaacact agaacattct gattgcgcgt ttatggtcga taatgaagcc atttatgaga 660
tctgtcgccg taatttaact atcgatcgac caacatattg caaccttaac cgattaattg 720
gacagatcgt cagttcaatt actgcctcac tacgatttag tggtgcttta aacgttgatt 780
tgaacgaatt tcaaactaat ttggtccctt atccacgtat ccacttccca ctaacaacct 840
atgctcccat tatttcagca gagaaagctt tccatgaaca actgagtgtt tcagaaataa 900
ctaactcctg ctttgaacct gcaaatcaga tggtcaaatg tgacccaaga aatggaaaat 960
atatggcatg ttgtttgtta taccgtggtg acgttgtgcc taaggatgtg aatgccgcaa 1020
tcgcgaatat aaagactaga cggtccatcc agtttgtcga ttggtgtcct acaggtttca 1080
aagttggaat caactaccaa cctccaactg ttgttccagg tggagatcta gcaaaggttc 1140
agcgtgctgt ttgtatgctt agtaatacga cggcgatatc ggaagcttgg gcccgtttaa 1200
atcggaagtt tgatctaatg tatgctaaac gtgcatttgt tcattggtac gttggtgaag 1260
gcatggagga aggagaattt tcagaagctc gcgaagactt ggctgctctt gaaaaagatt 1320
acgaagaagt tgcttcagat actgtagatg cacagtctga tgatggattt taatgtaata 1380
tgattacaat ttacaaaata aatttttata ttctgtattt ctctaaaaaa aaaaaaaaaa 1440
aa 1442
<210>2
<211>1276
<212>DNA
<213>日本血吸虫(Schistosoma japonicum)
<400>2
tgtggttatc ctatcccatg atgcctgcct atactaatgg tggaaatgaa gagttggaaa 60
ccttcctgtt cacttctgaa tctgttggtg aaggacatcc agataaactt tgtgatcaga 120
taagtgatgc catacttgat gagtttttga agcaggatcc cgatgccaaa gtagcatgtg 180
aatcgtttgt caaaagcggt cttattattg ttgggggcga aatttcttct aatgctgtga 240
tcgacattca gaagaccgtc cgtaatgtcg ttaaagacat tggctatgat gatactcgca 300
aaggtcttga ctataaaaat tgcaacatct tagctgtgat tgaacaacag agtgcagata 360
ttgcgaaagg tgtgcatgaa ggaagaaatg ttgaagacat tggtgctgga gaccaaggtt 420
taatgttcgg ctatgcgact gacgaaactg aagaatgtat gccattaacg gttaccttat 480
cacatgcttt aaataaacgc ctggctatgt gcagacgtaa tggcattttg ccttgggccg 540
gtcctgattc aaaaactcaa gtgactgttg agtatttaaa tgaccatgga gcatgtcgac 600
ctttacgagt acatacagta gtcatatctg cacaacacga cgagcatgta accttagaga 660
aagttcgtgc tgatttaatg gaatacgtta ttaaagagga aatacctgct aaccttttgg 720
atgaaaaaac aaaatatcat ttgaatccag ctgggagatt tgtaattggt ggtcctcaaa 780
gcgatgctgg tttgacagga agaaaaatca ttgtagatac atatggaggc tggggtgcac 840
atggtggcgg agcgttttct gggaaggatt actcaaaagt agaccgatct gcagcttatg 900
cagcacgatg ggtgtctggt ctttgtagac gtgtcttggt tcagctttct tacgctatcg 960
ggattgctga accattgtct attaacgtaa acagttatgg aactggcaag attagtgata 1020
agaagctact ggatgttatt atgaagaatt ttgatttgcg acctggtata atagtcaagg 1080
atctagacct tcgaagacca cgttacttac aaacctccgt ctatggtcac ttcggtcgac 1140
ctgaatttcc ttgggaacaa tgtagaaagt taaaattgta atattgacga ttttgtccag 1200
ttccatttct attttagaaa tatctattca tatgaatata aataataaat tggaaattta 1260
aaaaaaaaaa aaaaaa 1276
<210>3
<211>1492
<212>DNA
<213>日本血吸虫(Schistosoma japonicum)
<400>3
gattcatgaa cttttaaagt gacagctagt taccccacac ttttattgcc cgggtttatt 60
cagatcaatt acttccatgg ctgaaataaa gtatcgtagg tgtttgtcgc tttcaatcta 120
ttgttagtgg cgaagtactg tcgatatttt cagtatggag cagtacattt catacagtct 180
atgaaaacct aacgtaatca gtactatacg ttcctttcta gaacacctgc tctcattcag 240
ggatttttta ttttacatca cttcttttct agtcccgaat ttcagtaata gtaaacaatt 300
tatgttatga aatccatttt gcaagcatag atattgaatt ctgtgaattt ggttgtttct 360
catacaaatt tacttcaata tcggttatat ttagtcaaaa cattttagaa attgttaacc 420
taactattca ctatcacttc atatgaatca tacaagagca ttacattttg ttttcaaagt 480
agctaatcgt aatgaaacaa tagattttta caaacgaatt cttggaatga aggttttaag 540
acatgaagaa ttcagtgagg gttgtaaagc ttcctgcaat ggaccatatg atggaaagtg 600
gagtaaaact atgatagggt atggtccgga agataatcat tttgttattg agttgactta 660
taattatggg gtcggtgctt atcaacttgg taatgatttc caatatatac gtattcacaa 720
caaggaagcg tattctagaa taatcaatgg atcttggcca atcacgtcag aagaatcaga 780
ttcagtaaag gtgaaagctc ccggcggata tacattttgg gtgcataact ctgatagcga 840
tggggatcca gttcgaatgg tggctttaag tagttccgat attaaacgat caattgatta 900
ttggtcacac aaacttcata tgacacttgt atcatccagt tcaaatgaag ccgtatttta 960
ctattcaccg aatcaatgta atttgaagtt aatttctatt caaaagcctg ttgatcatgc 1020
tagtgctttt ggtcgtatag cttttgcatg cccaagtgct caattaccta ttttgcaaga 1080
attaatggac aaagaaaatg agaccattct tacgcgttta gtcagtctgg acacaccagg 1140
aaaggctaca gtacaagtta ttattctagc tgatccagat ggtcatgaaa tttgctttgt 1200
tggagatgaa gcatttagac aattatcaca agtagattca caagctgata gtctacttca 1260
aactgctata acttcagata aaagtgatga atggtacaat aaacatggtg gaaagacaac 1320
aaaatgaaat gaaatacaat ttcatttgaa ctcattcaat taagtatact aatggtggga 1380
taatttagtc actatttttt gtaccttttt ctttttcata cggaatattt gattttcttt 1440
gctttaatga agaaattatt gttgattctc ttacaataag tcattagata gt 1492
<210>4
<211>757
<212>DNA
<213>日本血吸虫(Schistosoma japonicum)
<400>4
taaatatatg tatatatgtg tgttcgagta tggagaaaaa atatgtagtt atccaataac 60
aaggtattga ggataaagat gaagttgcat tgattgcatt ttgttacggt tatgtttaag 120
agaaacacat tattgatcag tttataatga aattgtgtaa tagtctatca tgttgtagga 180
gtttttggct tggcgaaacc caaattactt tgattaaata ccacacttcc tagattacca 240
tagctttcaa tgataatttc accattagta aactgaacat tcataccaga acaagcttct 300
ttcaaagctg caagaaaatt gttcacatca tatacagata ctggattaga taaatcttcc 360
agccgctagc atcaaaccgc tcgaaaaagt cgctgatggt ctcattaaga aagctaaaga 420
ccagaatttg agggttaaag gccctgttcg aatgccgaca aaagttttga gggttactac 480
gcggaagact ccatgtggtg aaggctcaaa gacatgggac aggtttcagt taagaatcca 540
caagcgtgtg atcgatttac tgtctactga cgaagcgaca ctacagttga aacacatcta 600
tttagagcct ggcgtcaact gtgatgttaa agtttcagga gatttatagc ttgtaatata 660
attatgtgaa aggatctcta gacttttgtt aaagtgcttt gttacatgta gaacttaccg 720
tcgtagtgaa tccattcaat aaaatattca ccctatt 757
<210>5
<211>1756
<212>DNA
<213>日本血吸虫(Schistosoma japonicum)
<400>5
ttgacttatt agacttatca aaccctgacg ctaaaccttc aaattcttcg aacaatatag 60
ttcagaagcc tactcatgtt actgaaaatg gcctaaaata caatcatgat agcaatctta 120
cgcattcggt gtcacctcct aaagaacaac tattattaca gttagatgtt actcaagctc 180
gtcttgttag gaattatggt ttcctcccta tgaaccttta ctgcataatt cgcattggag 240
atgccagatt cgaaactcag acttcatcaa gatctggtaa acatcccatc tggaatgaaa 300
cttttcgatg ttggctccat gatggtgttg acaaattatc attagaaata gtttcggaaa 360
atatattttc gtcttcacaa gtcgctcatg ctgtaatagc ttttccacga tcgttatatg 420
atggtatcgc tttaaatcag tggtttgagc ttagtggaca acaaggagaa aacttagaag 480
gttcaattaa tctgattttc caatgtcgaa aagtaccagt aaatctccca cttctccaat 540
cgactccact atactgtcaa ccaagattgg gtgtagttgg tgatatgcaa gttttaccta 600
aaccgatatc tactgaagaa gttcagtcaa tacatgaaat cttcccatct gtcgaagagg 660
atacaattcg atcactttta gcaacacatc atggaaatcg tgaaattgta atcagcgaat 720
tacttctatg gcatcggaaa cttgaaatag tatgttcaga ctgtgatgta tttaaatcat 780
tttcaaataa tgttattttt ctgttcattt gttattggaa cgatttttct tctgaacaac 840
gttttccgct cacagcacta acgtgttatt accctgtttt acttcaacct cctttatcaa 900
actttttaac aaaaataaaa cagcgttatg atcatttcta cactgatttt atctaattca 960
attatcagtt attatttgtt tgttttcaca cagttacagt atatgtgcat tctgtgcgta 1020
tcttcatcct tctttcctaa tacctaatta ttaagatcat ctcaatgaaa cgacactgta 1080
gttataatgt ttatcacgta tgtatatttt attttgttgt ccatgttgtt cttattttcc 1140
attttttcta ccaatttatt ttcacctcta agcatatcct atttttgtgc tacatcagtt 1200
tgttatatag ccaaaacttt agtgaattca atgatagtgt ctatctcttt acaactgtca 1260
ctactgtcgt tgtaactgtc actcttttgt cttctacaaa taatgattgt gtagtttata 1320
tatatatata tatatatata tatatatata tatatatata tatatatttc gaatcatcaa 1380
cctatggatt gaaactaact tctctaacta ccgtttcatt tacttaccaa atatagattt 1440
ttgtatactc tgctttaagt aacattgttt gtgtacgaac aattgtctta aatatactag 1500
attgaataag gttagtaact tcaaacccat tgatcgaaaa tgaatacttt ttcaaatact 1560
tggacactac tctccattta tgtatagtag gcttatggaa ttgctagtca tgtccccact 1620
ttgtatcctg cctagagcct ttaggctcca cggtaaatgc ttaaccctct gtccattgaa 1680
cctatatcca gtgattgaca actcacagta tatttctcag tcttgtccct gccattggtg 1740
agtaccgatc acctat 1756
<210>6
<211>1760
<212>DNA
<213>日本血吸虫(Schistosoma japonicum)
<400>6
tttgtaaacc acaatacggc tgacatcaga gcccatagtt tcaaagagct ccacctcaaa 60
cttaagagat ttcccatcca tgtacgcata ttcaggcgac gcaatcaatt cacaaacttc 120
tcccaacctc atagttgcaa caccgatatc ccaagcttta attacactac ctttcccaat 180
tgtaaattca aacttttcat ttcgggctct gcttgagtcg aatacttcac catgcttttc 240
accaccgaag ttagtaccga cataatgtac tatgaccgtg tcatcatcgc atggtttgat 300
gtccgagtag ccttctcgta ctactttctg gaaacattag tggaatgaac aaggacgcag 360
ttgacatgtc ggagacgcca aagcagtctg aagaggaata tctaaaagat ttcatagact 420
taagcccatc tggtgatcgt ggtattctca agaaagtagt acgagaaggc tactcggaca 480
tcaaaccatg cgatggtgac acggtcatag tacattatgt cggtactaac ttcggtggtg 540
aaaagcatgg tgaagtattc gactcaagca gagcccgaaa tgaaaagttt gaatttacaa 600
ttgggaaagg tagtgtaatt aaagcttggg atatcggtgt tgcaactatg aggttgggag 660
aagtttgtga attgatcgcg tcgcctgaat atgcgtacat ggatgggaaa tctcttaagt 720
ttgaggtgga gctttttgaa actatgggct ctgatgtcag cagaaacaaa gatgggagta 780
ttcgcaagtc aattattaaa aagggaagag atatccacaa tcctgtagct ggggctgaag 840
ccactattgt tttccgcaat cttttgaact tgacggagga aacagaagtc acatattgtg 900
ttggcgaccc tccatcaaac gtaccggatg agttggacca atctgttcgt cacatgaata 960
caggtgaagt cagccgtatt gtggtttaca aagacggtca ttcattaact tctggagatt 1020
cggataaaga tagaatagtt tacgaactaa cactgaagtc ttttgaaaag accaaacacc 1080
tctctggtat tacgtctttt ccagagcgaa tagcctatgc aaatacactt aaagagaagg 1140
ccaacaattt cttaaaggat tctaaatttg attcagctat cgaattatat aaacgtttgg 1200
acgatgagct gcagtacgta gtggctaatg ggcctacaga acaaaaggaa ttgtctgggg 1260
tcactgtagc tgtccaactc aatttagctc tggtctatct gaagctttgt aaaccagaca 1320
aatgtattga gttttgcaag aaagtgctcg ataattttag cgacaacgaa aaggcgctat 1380
ttagaattgg tcaggcacat ttactacgta aggaccatga agaagcagtt gtttacttca 1440
aaaggattgt aacaaaaaat cctaacaatg catctgctgt aaaacaggtt caaatctgtg 1500
aggaagagat tagaagagca aaagatatag aacgaaagaa gtttcgtcat attttcgaac 1560
gttgtaaaga cacgggatta aatgatgctc aaaatcatga ggatggggtt gtagtgaatg 1620
atgaaaagtc tgccctgtga gagcttaatt ctgtgtgtgg catgaagtta gcaccccgtg 1680
attttgtaaa tgtttcttat aattactggt ttaatttcaa agttgtgaat acattttaaa 1740
gcaaacaaaa aaaaaaaaaa 1760
<210>7
<211>1758
<212>DNA
<213>日本血吸虫(Schistosoma japonicum)
<400>7
ttcgttttca tctcgtcatg ctgacaagtg gtgatagtga tctggtgggg gaagctgtta 60
agtttcacaa accaggggag aactacaatt ctcctggtta tgttgtgaca aaaaatacgt 120
tgcggttatt aaaggagcac ctgaaacaaa cgggtggtaa ggtagtcacc aggtttcccc 180
ctgaaccaaa tggtatttta catattggtc atgcaaaggc aatcaatttt aattttggct 240
atgcaatgaa gaatggtggt ataacttact tgcgatacga tgatactaac cctgaaaaag 300
aagaagcaga attttttgaa gctattgagg atatggtcag gtggttaggt tttacaccat 360
ataaaattac gtacgcatcg gataattttc atcagttata cgaatggtct gtagaagaaa 420
taagaggttt taatccacct ccttctccat ggagagaaag gccggttgaa gaatctcttc 480
gactgtttga agacatgaaa aatggaaaga tcgatgaggg aatggcaact ttacgtttga 540
aagtgacgtt gggagatgga aaagtggatc cagtagctta tcgtatcaaa atgactcccc 600
accaccgtac tggtaatgaa tggtgtattt atcctactta tgattatact cattgtttat 660
gtgattccat cgagaatatt acacattcat tatgcaccaa agagttccag tcacggcgtc 720
cagcctacta ttggctttgt aatgcgctag atgtttactg tccagttcaa tgggaatatg 780
gtcgtctcaa tttgtattat agtgtagtat ctaagcgcaa aattttaaag ctgatagaag 840
ctggcattgt tagtgattgg gatgatccac ggttagtaac actgacagca cttcgtcgtc 900
gtggatttcc gcctcaagct attaatatgt tttgtgaacg tattggcgtt actatggccc 960
aaacaatcct cgatccttct gctctagacg cttgcgtacg cgactatttg aatgatcatg 1020
ctccacgtgt aatggctgtg ttagagccag tgttggtaac tattactaat tggtttgaat 1080
tatacggcga taagtcatct atcgagctta cagtagcgga ttttcctgcc gttccggata 1140
gtaaaactca cagcattact ttgcaacatg atctatacat agagaagacg gattttcaag 1200
aagttgctga gaaaggatat cgtcgtttaa cgcccactca ggcagttggt cttcgtcaca 1260
caggccttgt tttagaattt tctgatttta aaaaggattc taatggggaa atagttcatt 1320
tattcgtaaa agccaaaaag gttgaagatt ctattaaacc aaaagctttt atacactggg 1380
tatctaatcc gttgcagttt gaagctcgac tgtatgaccg attgttcact gtcaaggaac 1440
cagaaagcgc aaaggaggga ttcttgtctg tcattaataa agattcatta gtcgtactct 1500
cggatgcttt gattgagcag tctgtcatga atgcaaaagt ttacaccgct tatcagtttg 1560
aacgtattgg tttcttttct gttgatccag atacaacctc gaaacgcatg gtcttcaata 1620
ggactgtagc tctcaaagtc gatcctggta aaacaatttg atgtgaaaag catctcagtt 1680
atcataaatc attaatgacg tgttgaacac tatgtgtaaa gtgtcatgta atttgtaata 1740
aatttcttac tctggatc 1758
<210>8
<211>932
<212>DNA
<213>日本血吸虫(Schistosoma japonicum)
<400>8
cgatatttcc gtcgcattaa tacgggatca gaccacgtgt tttagtgtgc gacaatgttt 60
taatacttaa acgttatggt gcaccagtga ttttgcgggg ctggatggaa gagttaacta 120
cttttgttat acggaggcga gcatattggc cgtgtagaat aataccgatc tggcgacgtg 180
cactccacat agatagcgat accaaagacg aggggtcacc cctcacagtg gtgaacgact 240
cgtgaaatcg aagtggtctc cccgggcgcg cgctaatttg ggccgccacg atgcgagtgt 300
tcaaggatgc tatttctggt gatgagatgt tttcagactc tcactctcct cagcttataa 360
acgatgtcgt gtatgaagtc gatgccaact ttataacagt gtctaatggt cttgatagca 420
aattgatcgc tgctaatcca tcaggtgaag aaggccaaga agaagtatct gattccactg 480
aaagagttat tgatctagtt catgcgagcc gactagtcag tacgtctttc gataagaaat 540
catatagagc atatctcaag ggctacttga aagccataaa ggaacgttta caaaaagaga 600
atccagaaag agtcagtata tttgaaagtc gaatcaacga atacatggta aatgtcttca 660
aaaatttcga cgattatgag cactacattg gcgagtcaat gaaccctgat ggtatggtag 720
cactgatgaa ctttcgtgag aatggtgtga ctccctactt tgttttccta aaagatgggt 780
tgattgaaga aaaatactaa gtcgcttcag gtcattatcg gaattccgaa ttcatggaaa 840
acatcttgta aatcagtttc aatatacaat attttctgga taaaaaaaaa aaaaaaaaaa 900
aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aa 932
<210>9
<211>951
<212>DNA
<213>日本血吸虫(Schistosoma japonicum)
<400>9
atcatttaga atggataaga tttatttatc tggaattcac acattacgat attacagtga 60
gatgaattga gtggtgtgtg ttgtatgatg agaggaatga gagcagtatg acacaaatga 120
atgttggagt gatatagatg ggcatgaatt gatgagtgat gaactcaata ataggagggt 180
gcataactgc ctcctttgcc tccgttacca cctttgccac caccattctt accaccacct 240
cctttcccgg cattaggtcg tttgcagcat gggtattgct cgagatcgca ttcataaaag 300
agctaaaact ggcgctcgcc gggaacgaaa tcgcaagaag aggaaattcg agttgggtcg 360
cccggcagca ctgacaaagc ttgggcctaa acgtattcac accgttcgtg tcagaggtgg 420
aaatcttaag ttacgtgcaa tgcgcttgga tcagggaaac tttagctggc catctcaagc 480
tatttcacga aagacgaaaa ttattgatgt tgtgtataat gcgtcgaaca acgaacttgt 540
ccgaacgaag actcttgtca aaagcgcgat agttcaaatt gacggagctc cctttagaca 600
atggtttgag gctcactact tgaaagagct cggtcgccgc aaagttgtta gtaaaaaagg 660
acatacagta acacaagaaa atccagaaga agatattttg ttgaagaaaa gaagcaaatc 720
agcattgaaa aagtatgaaa ctcgtcaggc tttgcctcaa gcaaacgtag aagagtcttt 780
gaaggaagaa tttgttacag gtcgactctt agcttgtatt tcctctaaac ctggtcaggt 840
tggccgagca gacggctaca ttcttgaagg aaaagagttg gaattttact caaagaaatt 900
aaaagctaag aaagcaaaat aaatttctct tataataaac ttctgtagga c 951
<210>10
<211>624
<212>DNA
<213>日本血吸虫(Schistosoma japonicum)
<400>10
aattcggcac gagggtttgc gcgggttact tgttatcggg cgtgactgtt ggtagttttt 60
caagccacta agttttaaga tgaagttcaa caaacatgtg agtagctctc gtcgcaaagc 120
tcgaaaacgt tacttcaacg ctccttctca tattcgccgg cgactaatga gcgctccttt 180
atcgaaggag ctgcgatcaa aatacaaagt cagaagtatg cccattcgca aaggggatga 240
agtacagatt gtgcgaggtg agcggaaaga ttgcaatcct gcaaaggtca ttcgtgtata 300
tcgcaaaaaa tatgtaattc acatcgagag gcttcagtgt cgtaagatga acggggcata 360
tgcacccatt ggcattcatc cgtccaatgt tgtcatacat aaattaaagt tggataagga 420
cagacgctca ttgttggagc gcaaggctgc cggtaaactc gctggacagg acaaaaacaa 480
atacacagaa gaaactattg agtcgtaatt tttattacat gcttttgtgg taaaaaaaaa 540
aaattaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 600
aaaaaaaaaa aaaaaaaaaa aaaa 624
<210>11
<211>456
<212>DNA
<213>日本血吸虫(Schistosoma japonicum)
<400>11
tcggattgtg ttctagaaga catgtctgct cgtgcattta gaaagtttgc tcctctgttt 60
gaccgagtat tggtccaacg gtttgaagct gaaaccaaaa gtaagggtgg catcatgttg 120
ccagagaaag ctaagggcaa ggttcttgaa gcaactgtag ttgcacacgg tccaggagtg 180
aagaatgaga aaggtgaggt agttccagta tgcgtcactg ttggtgacaa ggtttttctc 240
cctgaatatg gtggaactaa agtcgtactt gaagataccg agtactttct tttccgagaa 300
tctgacatct tggctaaatt tgagaagtga ccggtgtctt agatctagtc tatctaccct 360
tgtacaatga ttattccaat tacatagttt ttcatctcta aaaaaaaaaa aaaaaaaaaa 420
aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaa 456
<210>12
<211>968
<212>DNA
<213>日本血吸虫(Schistosoma japonicum)
<400>12
agtgtgccat aaagcttgtc taactgccga aatgctgcat ctgaccaaat agctaaacgt 60
cccatatgac cacccggagc cagcttaagg agatttaaag ctctcacgtt aatcaaaggt 120
gacacctgga atatttctaa aagcacgcga ttatcccatc atccttctta taaattataa 180
ttgggccacg tttttgaatt cgccttcgat ttctcatttc tccttttaat ttacaagtgt 240
gatcacgagg atgtcgttgt gccgacaaaa ctaccatgag gaatgtgaag ctggtattaa 300
caagcagatt aatatggagc tctatgctag ttatgtttat atgaccatgg cttttcattt 360
tcatcgtgat gacgtcgcgt tgaacgggtt ttataaattc tttcttaatg agtccgaaga 420
agagcgacaa catgcgataa agttaatgac gtaccaaaat atgcgaggtg ggcggattgt 480
gttgcaagat atatcagcac ctcctcaact ttcatggact tcaggtctcc atgccatgca 540
ggatgctttg gaattggaga aaaaagtcaa tcaaagtttg atggatttag ttgccgttgg 600
tgagaggcat cgggatacac acttttgtga ttttattgga aatgaatatt tggaaactca 660
aatacaatca atgaagaagc ttgctgatta cattacgaat ctcaaccgtg tgggcactgg 720
tttaggagaa tatactttcg ataaagaaac cttacacgga gaaagtgatt aatttcgtgc 780
tttcaagtag ctgtttgcgg attgtaataa actatttttt gtaaaataat ttatgtagat 840
tgatttacgg tattcttcag cgaaattcat caggttgcaa actagttaca tgtaatgtca 900
gaaaagtgtt tcattttttg ttattgatta ttacaatatg accaaactaa gtcttctttc 960
taaaaaaa 968
<210>13
<211>616
<212>DNA
<213>日本血吸虫(Schistosoma japonicum)
<400>13
atacgatgtg taccagagcc agttgttgga ggaccgtcat gcacaatgtt ggctatcaag 60
tcgtaagtag tgtaccgatg tttagctcga aattctgaag acaaaagatc acctaaatca 120
atacttttaa tggggaaatt tacaattgtt ggatttttct ccagtgtgaa gatgttttta 180
acaaatctct tcatatagag aattaaataa ggaggaagtc gacgaagctc aaaccgacgc 240
aacgtagaat cacggtgagt tttatattcc ttctccgcaa ttccattaaa ttttgaaagt 300
agtgtagctg ttaatatcca aggtcgtaac atggcaaaga gcaagaacca tacaagtcat 360
aatcaaaata aaaagcaaca ccgcaacgga atcattagac cgaagaagca gcgatatccg 420
tcacttaaag gcgttgaccc caagttcctt agaaatatga ggtttgccaa gaagcacaat 480
aaacgtggga ataagaaggt acagcctgca gttactccaa cagtagcggg atgtaaataa 540
tcgtggattg cactcgtaat atgtaaggaa taaatatact tatataaatc acaatgatcc 600
agttcaaaaa aaaaac 616
<210>14
<211>432
<212>DNA
<213>日本血吸虫(Schistosoma japonicum)
<400>14
tttagcgcca ttgtttactt cttgtttact tgacacgtat tcacatggcc gataaatcac 60
ctgagaaaaa ccatatttca catcctccag cagtgaaagc tggagggatg cgaattgtga 120
aacatgctaa agaacacgag gaaagtgtgt tatcgaagtc agatctagcc aaacaagcgg 180
ttgaatataa ttctagtatt acaggtattt catcacacga tcttctcact aaggaagaac 240
ttgaatctca tgatagatgt atcaaacttc atcaggaaaa gccgcttcca acagttgcaa 300
aacctcataa caacgatagt acgagacgtg ttattcaaca acctttaaag tgaataacat 360
gttgttttta aagaatgaag ttactctttt gtaaacttta ttttttaaaa taaaataagt 420
taatatttat tt 432
<210>15
<211>1394
<212>DNA
<213>日本血吸虫(Schistosoma japonicum)
<400>15
attgaaacca ggtgtttcgc tcattacaag agtcgacgta cttgtccagg atttacgctg 60
tctgtttcgt tgcagcattg aacttttcct cctgatcgtt ttgctttatt ttttgcatag 120
tgtattttta attgttgatc ggatacccaa aagcattgag cgtacttccc tcttagattg 180
ctaagtttgc taggatcact cctgaaagtg atcagttggt tttcttcatt caaccttttc 240
ccagagctta cgtgcctacc tcactactcc gagactttat tttctcatgt tttacagtat 300
caagctgtga cacatgagac acagccacct ttttgtctgg tcgtgttacc ctctgagtac 360
gttgtttgtt agtaaaagaa tgagtgtata aaacttgaaa aacgtattta agcagcatct 420
taggtttcac acgtttgtaa ttctgtttgg ctttattcgg cttttgttta ttgttgtctt 480
gttccgtttt ctattccatt tttctaacct ttagatcact tgactgaata cctttcaaga 540
gcatcagaca cttggtccta catttgctgt tatcgctgag tacatttcta agtgcatctc 600
gttttgagtc ttattctttc cctgatttct tcgtaaacac tgctctgcat tttatcccag 660
tattggacac cttcatacta gtcgcacagt tgctaaaggc ttaacttcac atgtagcttt 720
ccgtgatgac gtctgaaaca gtgaatataa tggacttaac cattccacaa ctccaagagc 780
ttgcgaggca aatggagcaa aaaatacaat ttatatctgc ttccatacag caactaaagt 840
cacttcaaac ccaattcatt tcttcgaaga attatctgtg cgaactgact ccagacaaag 900
agaatagcga tattcttgtt cctttaactt caactttgtg tgtacctggg acactttccg 960
acgcttcaca tatatttgtg gatatcggta cagggtatta tgttgaaatg accgtgctag 1020
aagctgaaag tcactttgac cgtcgagtgg aatatatcaa taagcaaatc agaaagattt 1080
ttccagttct ggaggagaag actcgtgttc ataagtccat ttcagacact ttagatgcca 1140
aaattcagga tttcattaag atccctccat caccgtgctc atagtatagt aagatgaact 1200
tttaggactt gcacaaattt ccactattac tccgctgttt taccaatgaa gattgtgtga 1260
aactaatgat gtagttggga tcatgccctg attattataa agtatgcaaa atttattagt 1320
aactgagtct taaatctaaa tgtttatttg aataaatggt gaatcggatt ggtcaaaaaa 1380
aaaaaaaaaa aaag 1394
<210>16
<211>561
<212>DNA
<213>日本血吸虫(Schistosoma japonicum)
<400>16
tttggagaac tatttgcact tcatggtatt gtagaaacca atcgccacaa attcaagata 60
cggtttaagc gagattttgg caatgacgag ctcattttat ttgcgctact acgtgggaca 120
caaagggaaa ttcgggcatg aattccttga gtttgagttc aggcctgaag gcaagttgcg 180
ttatgctaac aactctaatt ataaaaatga cacaatgata cgcaaagagg catatgtgag 240
cccttctgta atggaagaac tgaagagaat cgtgctggaa agcgatatta tgtcagaaga 300
tgatgccaca tggcctgtac cggatagagt tggacgtcaa gagctcgaaa ttgtttgtgg 360
tgatgaacac atatctttca caacctcgaa gataggctct ttgattgata ttaccaacag 420
caaggatccg gatggactaa gaacgtatta ttacctggtg caagatctga agtgcctagt 480
gttttcactg attggattgc attttaaaat aaagccaata taattcattc ccttcaaaaa 540
aaaaaaaaaa aaaaaaaaaa a 561
<210>17
<211>1600
<212>DNA
<213>日本血吸虫(Schistosoma japonicum)
<400>17
atatttatgt tgtctaacgg tgttacttcg ggtaaaatgt catgtgcaga cagttgtcct 60
atggccgaga aagtacgctc tataatatat tggcggcatc caattataac aggcgttata 120
ctgactcttt tactgattgt tgaatttagt tttatgtgtc tttctgcaat ctctttcatt 180
gcttatatcg ggttatcact ccttgtttcc gtcaacttag tgcgtatgta ctatcatttt 240
gtcgccaaaa cagaaaataa ttttgtcaga gaatacctgg aacgcgagat cactgtgcct 300
caagacaaag cagaaaaagt ctctcgacgg ctgaccgaag ttattaacaa gggtttagta 360
cgaactagag aaatattctt attaactgac cccgctgcat ctataaagtt tgctgtacta 420
ctgtatctac tgacatacat tggagctcag ttcaactttc taactttatg catactgttc 480
acttttggta tcttctgtgt gccaaagttt tatgaacgct atcaaccgga aattgataaa 540
atactggagc tagcaaaatc taaaatagat atggtagtat cacaagtaaa atctcaaatt 600
gaaaagctcc cgattattgg cggaggtggt cgtaaagaaa aagcacagta aattaaacgt 660
ttccagctta tctaaatata tttcgtacca aattcgatga gcaacgatac aatttgttca 720
aagcaattta caataaaatt cccttttctt cctataagta taataaatgc ataaaactgt 780
aaagcatata aggaacaatt agttttacat tctttctctt ctccttgttt tgtgtgtgtt 840
tcctaccgat ttttatatat cgtgctttct gtctacaaaa attctctcta acgttgttat 900
tcattcaaca aaaatttatg ataaattctt aattgtttct gtttgttgga gatttgggtt 960
ttcattatag taatctgtgt attaatgaat agagacgatt attaaatatc tggtatcttt 1020
caaaataaac aatgttcatc atccccctct gtcatatttt actgtgttta gattatccat 1080
ttgttgtaac aacgctaatt tctttttctt tattcctaaa ggaattgtcc tatcatgtta 1140
ctacaaaaca taattgaata ctgtttccat ttgataaaat tctatgtacg tattatctag 1200
atgttactaa ctgcacaatg tttagcctac tttctttttt tgttctctcc gtggtaaatc 1260
ctacttcttg ttgtccatta cagttttgct gcagtggttt ttttttcttg gttccattac 1320
tgttatcatt tactattcaa atattgtgtt tggtattaca atgagcattt aatgacatta 1380
acattattgt attggactaa ctaattggtc aactggttgt tttactacca ttattatata 1440
gagaatgatg ttaatctctt ttgaacgtga tttatatttg catagttatt gttttcgctt 1500
cggaattcca tagatatggt tcataaagct aatgagttac tttgaaacga agtaatctat 1560
cttcgttaaa acaaaaactt acacttagta ttttccatct 1600
<210>18
<211>928
<212>DNA
<213>日本血吸虫(Schistosoma japonicum)
<400>18
aggctaatca tctaagcatg cctaataagc aaaaaaaggt tattaaagcc aagaaaatta 60
aggaaatagt tcatcatgaa cctgatatac cagttcttcg aaaaggaaag gcttcaaaga 120
ttgctcccct tccagatatt gccaagaaaa cgtctgttaa gaagaaagtt attaagaacc 180
cactaatcca acgccgacct aaaaactttg gaatcggcca aaacattcag ccgaaacgga 240
atctttaccg ctttgttaag tggcctaagt acatcgagct tcaaagaaaa aaggctatac 300
ttaaaaagcg attgaagatc ccacctccaa tccatcaatt ttcccagaca ttggacagaa 360
actcagctaa tcagatattt aacttagtta cgaagtatca accactgagt aaacaagcca 420
agaaaatgct tttagttcat cgagcgcaac ttcgtgccga aggcaaaccc gatcaaccaa 480
caccacgtaa acctactgtc cgtgctggta ttcgtgaagt aaccactgcc ataactcaga 540
aaaaggcacg cctagttgtt atagcacacg acgtagagcc tattgaaata gtattatttc 600
ttcctgctct gtgtagaaaa atgggcgttc cctactgtat agttaaaagt aaagcaaagc 660
ttggcctcct tcttcacaga aagacgtgta cctgcctagc tttcacaaac attagagaag 720
aggataaatc acgattgtcg aagatagttg aaatgggtga aaaacaatta caatgatcgc 780
tttgaagaga ttcgcaaaca ttggggtggc ggaatcatgg gttcaaaatc tctcgctaaa 840
caagctaaaa tcgagaaggc aaagagaaag gagcttcaaa ttaagcttgg ataatttatt 900
aactttaata cagtttaatg gccaattt 928
<210>19
<211>1008
<212>DNA
<213>日本血吸虫(Schistosoma japonicum)
<400>19
cgttgcgcaa cgcatgggtc gcaggaaagg acctaagcat gatcatcagc tgaagagtcc 60
aaacgtagaa cattccacgg aagaactaat tgcagaatcc tctgtcagtg aaaatttgac 120
tgaggcccct acgactaagt cacctgactt ggaggttact gcttgcagca cagagactat 180
tgacgtccta ggaaatggtc tcgtcgttat aaagacgtta agaaaaggtc tcggtaggga 240
aactcgacca agccacggtg atactgttgt tataaattac aaaggttggt tggaagatgg 300
gactcttgtc gatgatgttg aaaatgctaa aatagtactt ggcgatggtg atgttataca 360
tgcatttgat ctttctatcc ctttggccga acacaaagag acattcgaac tgattactga 420
tgcgcgattc gcctatggat ctcgtgggcg ggatccggat attccatcag gagcaaaatt 480
aacctatcac atagaaattc taaaagttga tgatcctccc tgttatgcca atatgccgaa 540
ttctgaaaga cttgcaattg ctaatcagaa aaaagataga ggcaactatt actacaggag 600
agaagaattt gcattcgcca tcgatagtta tagcaaagca ttgaaaattt tacaattacc 660
tacatcttct actcaatcat cgggggaaaa atcggatgta gattgtccga cagagcttat 720
taatgatgcg aagttaaaat tggaaaataa tcttgcagca gctcagttga aggttgaggc 780
gtttgatgcc gctataatgt cctgtgatac tgttttgcaa agcgaccccc aaaatattaa 840
ggctctgttt aggaagggaa aggctttatt agaaatgaat gaagtagatg atgcgatccc 900
aattcttcaa aaagtgctaa cattagctcc aggttcacag atggctagcg ttgagctagc 960
tcgtgctcga gctatacgac agaaggaacg cgaacattgg tcacgatc 1008
<210>20
<211>821
<212>DNA
<213>日本血吸虫(Schistosoma japonicum)
<400>20
gttttttcaa tagtctgtga catgcgtgca aatggcgagc tgaaattatt ttctgtcgta 60
ggccggaagc taccgacaac aaaggagcca aaacccgtcc cttacagaat gcggatattt 120
gctcctaact cggttgttgc aaaatcacgt ttctggtact tcattagaaa gctaaagaaa 180
atgaagaagg gtagcggtga gataatttct tgtgaaagaa tacacccacg aaagccattg 240
atggttaaaa atttcggtat ttggctaaga tatgatagtc gttcagggac acataacatg 300
tacaaggaat atcgtggttt gacggaggaa agtgcagtga ctcaattgta ccgtgagatg 360
ggtgctcgtc atcgtgctcg agcggagtct atccaggtaa ttaaagtgga ggcaatacca 420
gcatcgaagt gtcgtcgccc atatattact cagttccacg acagtaaact gaagtttcct 480
ctacctcatc gcgtggcccg tcctcttcat cgcccaaggt tcacaaccag aagacctaca 540
acagccttct agttttacaa taaatttgaa tggttacttt catttgttct tgattatgta 600
ttccgtgtta gattttgtat cctcactatt tcagtaaacg cgtattaatg cgaaattatg 660
gtttgttaat ggtgtataat ataggcagct cttcctcctc aactgtttta tctgccagct 720
tacgcgtatt caacatttaa ttggtacttt aatcatttca actgctttat ccagttgttt 780
gttcttatta ctttattatt ctgctaaagt atagtaaagg g 821
<210>21
<211>943
<212>DNA
<213>日本血吸虫(Schistosoma japonicum)
<400>21
gtgcaatctt tcgaatatgc ctacattaag ctgtggatat aagtgtttgc aattcttgtt 60
aatcaccttt aatatccttg tttttgcatg tgggattgct ttaatagtta ttgggagtct 120
ctctcaagtg gctgttaata actactcgac gggaatcgat tcaaatatca aaggactcgt 180
aatatttatc ataatactcg gatgctttct cttcttgctc ggatttcttg gattttgcgg 240
ggcatgcaca aaaaatactt gctgtttaac aatatatgct attcttttgt ccgttatggt 300
tgcggctgaa ataggggtag gaatagccgc tgccgtccta aaagaagatg tgaaaacaag 360
ctttgtgtct gtggttagga actctgtttc ggaatatagt aaaaatcctg atattcagaa 420
tctttttgac aagatacaaa cagagtttaa atgttgtggt tccgagtcat ctatcgatta 480
tacatcaact ggacaatccg ttccatcctc ctgtacggat tcagatactg ggcttgcgta 540
ccaagaaggg tgctcaaaca aaataattgc cttcttcgaa aagtatctta ttgctgttgt 600
ggtggcagca ttcgcattcg ctatccttca aatattaagt attgttttta ctgtctgtat 660
tattcgagct attaaatctg gtgatactga gtgaaactaa aaatcataaa cgtgtaaaaa 720
ctagctttga tttttcataa acttccaaat taatgaaatt aaactttttt tcattgtatc 780
tttattttgc tgtcatttaa cacttggaaa acggagccat attttttatt aaaacatata 840
ctagaaaact acttatgaat attttatggt tactggacta acggttataa taatgacaaa 900
attaaataaa atttgaatac tttcttaaaa aaaaaaaaaa aaa 943
<210>22
<211>812
<212>DNA
<213>日本血吸虫(Schistosoma japonicum)
<400>22
taaaaaagaa acaatgaaga ttttatttat ttgtctgcta cttattacac tacttactat 60
gactattcaa tataatctaa atcgaaaata cactaatcat tatcggagac aaccaaataa 120
tccttataat cgtaataata atggtaataa taattatgat gataacaaca ataatgacaa 180
caaaaatatg tatgataagt atagtgataa tagtaaatac ggtaattatt acccaactaa 240
taatgattat aataaacaga aaccaaaata tccacgtctt accagaagat acagtatgga 300
aggtgaagaa aagcatgcgc atcaaaaaca tggtgaacgt catttaaaat ctattggtga 360
attatttggt tttactaata caaatcaagg ttcagactac gcattaacaa caacatttca 420
taaaggtagt atcgctgcta aaaatggtaa acaaaagcat ttttctactt atgaaacaga 480
aggtcaagtg aaacactatc agaatacaca tggtcaggct gaattcaatc taaatgctaa 540
actatatggt caagaaaagt ataatgatca gaaggaaatg aaagcaacag taaaaatgga 600
agattatgat aatacacctg acgacgaagc aaattactca ccagtcggac atccatatgg 660
taaattagaa ggctactaat tatgattaaa aaaaacaagc gctcataata aatatgaaat 720
tataagtcag ttgaattgaa tgtatataac ttccatatta ttctcttttg agtcatgaca 780
tgttgaaaat cgaataaatc ttataataag at 812
<210>23
<211>1535
<212>DNA
<213>日本血吸虫(Schistosoma japonicum)
<400>23
aggagctgca gttctcacat ctactgcaag tctaggctgt gatccatttt tactgccccc 60
ggatcatcga agacttacaa accatcaacg atcatttgcc gctggctatt caagtgatca 120
cttggctggt ttgaatgtct ttcaagtatt ggcttctgaa cgtaaccgtc ggggtgatga 180
gtcagctgat tcattttgtg accaacatgg gttcaatagc tctgcgttac gaattattga 240
tgatgctgca aaccaactta gagctatcct tatcaatttg ggttttcctg aagaatcatt 300
gtctgatgca ccggttgatt tcaacgttaa acacaatatt gattgcgata taattaatac 360
tttgctggtg tctggattgt atccaaatat ctgttttcat tcagataaac gcaaattact 420
cacaactgaa ggtactctgg ctctcataca caaaggttct gtcaattgca tcaaagatat 480
caagttcact tacccatttt ttgtttttga tgaaaaaatt cgtactcagg ccgtttcatg 540
caaaggtctc acaatggtaa atccaattca actgatgctc tttggttgtc gctcagctgt 600
ctggaggaat agtgattcta atgataaagg aatagttgtt attgatgatt ggattccgtt 660
ccgcatgagc tttacttcag ccgctcggat ttttgctttt cgaccagcat tggaagcatt 720
gcttgtccga acttgcttgc gaccagaagg tattgctgat cttagagagg aagataagcg 780
tgttattaat gtattgcgtc aactatgctc accagaagct ttaaatccaa acgctagttt 840
cgatgaagca agcagttacg actctccacc gagaaaaaag tttgctacag ctggtaatta 900
tccgttactt tctggtcgat cgcatcgcac tgtgggttat gagaattcat gctcagagaa 960
acaaggtttt ggacgttatg ctaattgtcc tagtcggttt ggtgtctcag aacaagcatt 1020
cgatggtcag tgtggttttt ggcaggaccc tctttctcaa ttttcaaatc agaggtatga 1080
ccaatcatct gtcaacagat ttcctgggaa ctacacaagt atacggtcgt ttggcgctag 1140
cggtatggga aataatctta atggatcatc ttgttactcg tatggagtta ctccttttgg 1200
tggatacccg aatcaacagc cacagtttac aaatgatagg tttattcaac aaagtcaaag 1260
gccagtaaat agacctcatt ggggcatacg ttattagctt taatttttga aactgttttt 1320
aattgtatcc aagtattaga ttcttctcct aaactctgag tatttattta tttggactca 1380
tttctatatg tactgtattg atatccccat ctttctaaca tgctaaactt tgtttcttgt 1440
tgactaactt taatgacctg gtgtctaaac cttcatttct acgcactgtt ttgttattgc 1500
catctaagtt acaataataa aatcgttcta agttt 1535
<210>24
<211>1055
<212>DNA
<213>日本血吸虫(Schistosoma japonicum)
<400>24
acctgatatg ccgaattatg aagtattatc tccacaggtt gtttgcccaa catgcaccgg 60
agcaggctat gtgagaagag ggctgagtat gtggctttaa tccctttgac agaccatcgt 120
ctccggccgc gtagaatctg tcttaaattg tttggctctg tagtgatttg ctccttagtt 180
tttactctct tactgatttt tctctttcca cgaacaatat atttgcgaag ttcagtttac 240
aagttaatta ctgtcagtgc catcgccaat atgcaagata atgtgattga tctcactgtt 300
attaaccaga ttaatgtttc aaatccaaac tttgttcctg ccatggtaaa taaggtcatc 360
atctctccga aataccgttc agtggatcta caagagactg taaacaattc catccatatg 420
ttaccacttc gttccgaaac tttactgaat gtcagcacca agttacgctt cgagcataag 480
cattttgatg tcattaaatt gtgcttcatg aaattgcctt acttccacga tatatacgta 540
acttttacat ttcatatcac ttacatgttg ttatggcaaa gttttgatgc atatcttaca 600
attatgccat tggttgattg ttatccaact aatataaatt catccatgtt gatggtgtct 660
agttaaatgg tttatcattc ttgctaatca tcattgattc ttttttatat tgaatatgat 720
ttaaagagat ttttataatt tagactttct atctacgcaa ttattttcta ttgaaagtca 780
ctatgcttat tgacttttat ttgaatattt tatttttctc attgtgattt ttaatctatc 840
attgttcagt tgattagaaa gtgatagcga gcgcgagaga gagaacgttt tttccacatc 900
taattcctat ttgttttgca tttgttatta attgtatgaa tacaaaatat gaagtcatta 960
tgcttttgaa ttgttattat tgtattccaa ctgtttctac tatttcaatg tagtggtcgg 1020
atttatctat gttaataaac caattgagtt atact 1055
<210>25
<211>448
<212>PRT
<213>日本血吸虫(Schistosoma japonicum)
<400>25
MRECISLHVG QAGAQMGDSC WELYCLEHGI QPDGKVPSDS TIKNDNDSFS TFFSETSYGR 60
YVPRAVFVDL EPSVIDRLRT GAYKQLFHPE QMISGKEDAA NNYARGHYTV GKELVDEVLD 120
RIRKLSERCT GLQGFLVFHS FGGGTGSGFT SLLMERLSVD FGKKAKLEFA VYPAPHISTA 180
VVEPYNSILT THTTLEHSDC AFMVDNEAIY EICRRNLTID RPTYCNLNRL IGQIVSSITA 240
SLRFSGALNV DLNEFQTNLV PYPRIHFPLT TYAPIISAEK AFHEQLSVSE ITNSCFEPAN 300
QMVKCDPRNG KYMACCLLYR GDVVPKDVNA AIANIKTRRS IQFVDWCPTG FKVGINYQPP 360
TVVPGGDLAK VQRAVCMLSN TTAISEAWAR LNRKFDLMYA KRAFVHWYVG EGMEEGEFSE 420
AREDLAALEK DYEEVASDTV DAQSDDGF 448
<210>26
<211>387
<212>PRT
<213>日本血吸虫(Schistosoma japonicum)
<400>26
MMPAYTNGGN EELETFLFTS ESVGEGHPDK LCDQISDAIL DEFLKQDPDA KVACESFVKS 60
GLIIVGGEIS SNAVIDIQKT VRNVVKDIGY DDTRKGLDYK NCNILAVIEQ QSADIAKGVH 120
EGRNVEDIGA GDQGLMFGYA TDETEECMPL TVTLSHALNK RLAMCRRNGI LPWAGPDSKT 180
QVTVEYLNDH GACRPLRVHT VVISAQHDEH VTLEKVRADL MEYVIKEEIP ANLLDEKTKY 240
HLNPAGRFVI GGPQSDAGLT GRKIIVDTYG GWGAHGGGAF SGKDYSKVDR SAAYAARWVS 300
GLCRRVLVQL SYAIGIAEPL SINVNSYGTG KISDKKLLDV IMKNFDLRPG IIVKDLDLRR 360
PRYLQTSVYG HFGRPEFPWE QCRKLKL 387
<210>27
<211>294
<212>PRT
<213>日本血吸虫(Schistosoma japonicum)
<400>27
MNHTRALHFV FKVANRNETI DFYKRILGMK VLRHEEFSEG CKASCNGPYD GKWSKTMIGY 60
GPEDNHFVIE LTYNYGVGAY QLGNDFQYIR IHNKEAYSRI INGSWPITSE ESDSVKVKAP 120
GGYTFWVHNS DSDGDPVRMV ALSSSDIKRS IDYWSHKLHM TLVSSSSNEA VFYYSPNQCN 180
LKLISIQKPV DHASAFGRIA FACPSAQLPI LQELMDKENE TILTRLVSLD TPGKATVQVI 240
ILADPDGHEI CFVGDEAFRQ LSQVDSQADS LLQTAITSDK SDEWYNKHGG KTTK 294
<210>28
<211>65
<212>PRT
<213>日本血吸虫(Schistosoma japonicum)
<400>28
MPTKVLRVTT RKTPCGEGSK TWDRFQLRIH KRVIDLLSTD EATLQLKHIY LEPGVNCDVK 60
VSGDL 65
<210>29
<211>248
<212>PRT
<213>日本血吸虫(Schistosoma japonicum)
<400>29
MNLYCIIRIG DARFETQTSS RSGKHPIWNE TFRCWLHDGV DKLSLEIVSE NIFSSSQVAH 60
AVIAFPRSLY DGIALNQWFE LSGQQGENLE GSINLIFQCR KVPVNLPLLQ STPLYCQPRL 120
GVVGDMQVLP KPISTEEVQS IHEIFPSVEE DTIRSLLATH HGNREIVISE LLLWHRKLEI 180
VCSDCDVFKS FSNNVIFLFI CYWNDFSSEQ RFPLTALTCY YPVLLQPPLS NFLTKIKQRY 240
DHFYTDFI 248
<210>30
<211>431
<212>PRT
<213>日本血吸虫(Schistosoma japonicum)
<400>30
MNKDAVDMSE TPKQSEEEYL KDFIDLSPSG DRGILKKVVR EGYSDIKPCD GDTVIVHYVG 60
TNFGGEKHGE VFDSSRARNE KFEFTIGKGS VIKAWDIGVA TMRLGEVCEL IASPEYAYMD 120
GKSLKFEVEL FETMGSDVSR NKDGSIRKSI IKKGRDIHNP VAGAEATIVF RNLLNLTEET 180
EVTYCVGDPP SNVPDELDQS VRHMNTGEVS RIVVYKDGHS LTSGDSDKDR IVYELTLKSF 240
EKTKHLSGIT SFPERIAYAN TLKEKANNFL KDSKFDSAIE LYKRLDDELQ YVVANGPTEQ 300
KELSGVTVAV QLNLALVYLK LCKPDKCIEF CKKVLDNFSD NEKALFRIGQ AHLLRKDHEE 360
AVVYFKRIVT KNPNNASAVK QVQICEEEIR RAKDIERKKF RHIFERCKDT GLNDAQNHED 420
GVVVNDEKSAL 431
<210>31
<211>552
<212>PRT
<213>日本血吸虫(Schistosoma japonicum)
<400>31
RFHLVMLTSG DSDLVGEAVK FHKPGENYNS PGYVVTKNTL RLLKEHLKQT GGKVVTRFPP 60
EPNGILHIGH AKAINFNFGY AMKNGGITYL RYDDTNPEKE EAEFFEAIED MVRWLGFTPY 120
KITYASDNFH QLYEWSVEEI RGFNPPPSPW RERPVEESLR LFEDMKNGKI DEGMATLRLK 180
VTLGDGKVDP VAYRIKMTPH HRTGNEWCIY PTYDYTHCLC DSIENITHSL CTKEFQSRRP 240
AYYWLCNALD VYCPVQWEYG RLNLYYSVVS KRKILKLIEA GIVSDWDDPR LVTLTALRRR 300
GFPPQAINMF CERIGVTMAQ TILDPSALDA CVRDYLNDHA PRVMAVLEPV LVTITNWFEL 360
YGDKSSIELT VADFPAVPDS KTHSITLQHD LYIEKTDFQE VAEKGYRRLT PTQAVGLRHT 420
GLVLEFSDFK KDSNGEIVHL FVKAKKVEDS IKPKAFIHWV SNPLQFEARL YDRLFTVKEP 480
ESAKEGFLSV INKDSLVVLS DALIEQSVMN AKVYTAYQFE RIGFFSVDPD TTSKRMVFNR 540
TVALKVDPGK TI 552
<210>32
<211>169
<212>PRT
<213>日本血吸虫(Schistosoma japonicum)
<400>32
MRVFKDAISG DEMFSDSHSP QLINDVVYEV DANFITVSNG LDSKLIAANP SGEEGQEEVS 60
DSTERVIDLV HASRLVSTSF DKKSYRAYLK GYLKAIKERL QKENPERVSI FESRINEYMV 120
NVFKNFDDYE HYIGESMNPD GMVALMNFRE NGVTPYFVFL KDGLIEEKY 169
<210>33
<211>217
<212>PRT
<213>日本血吸虫(Schistosoma japonicum)
<400>33
MGIARDRIHK RAKTGARRER NRKKRKFELG RPAALTKLGP KRIHTVRVRG GNLKLRAMRL 60
DQGNFSWPSQ AISRKTKIID VVYNASNNEL VRTKTLVKSA IVQIDGAPFR QWFEAHYLKE 120
LGRRKVVSKK GHTVTQENPE EDILLKKRSK SALKKYETRQ ALPQANVEES LKEEFVTGRL 180
LACISSKPGQ VGRADGYILE GKELEFYSKK LKAKKAK 217
<210>34
<211>142
<212>PRT
<213>日本血吸虫(Schistosoma japonicum)
<400>34
MKFNKHVSSS RRKARKRYFN APSHIRRRLM SAPLSKELRS KYKVRSMPIR KGDEVQIVRG 60
ERKDCNPAKV IRVYRKKYVI HIERLQCRKM NGAYAPIGIH PSNVVIHKLK LDKDRRSLLE 120
RKAAGKLAGQ DKNKYTEETI ES 142
<210>35
<211>109
<212>PRT
<213>日本血吸虫(Schistosoma japonicum)
<400>35
SDCVLEDMSA RAFRKFAPLF DRVLVQRFEA ETKSKGGIML PEKAKGKVLE ATVVAHGPGV 60
KNEKGEVVPV CVTVGDKVFL PEYGGTKVVL EDTEYFLFRE SDILAKFEK 109
<210>36
<211>173
<212>PRT
<213>日本血吸虫(Schistosoma japonicum)
<400>36
MSLCRQNYHE ECEAGINKQI NMELYASYVY MTMAFHFHRD DVALNGFYKF FLNESEEERQ 60
HAIKLMTYQN MRGGRIVLQD ISAPPQLSWT SGLHAMQDAL ELEKKVNQSL MDLVAVGERH 120
RDTHFCDFIG NEYLETQIQS MKKLADYITN LNRVGTGLGE YTFDKETLHG ESD 173
<210>37
<211>69
<212>PRT
<213>日本血吸虫(Schistosoma japonicum)
<400>37
MAKSKNHTSH NQNKKQHRNG IIRPKKQRYP SLKGVDPKFL RNMRFAKKHN KRGNKKVQPA 60
VTPTVAGCK 69
<210>38
<211>102
<212>PRT
<213>日本血吸虫(Schistosoma japonicum)
<400>38
MADKSPEKNH ISHPPAVKAG GMRIVKHAKE HEESVLSKSD LAKQAVEYNS SITGISSHDL 60
LTKEELESHD RCIKLHQEKP LPTVAKPHNN DSTRRVIQQP LK 102
<210>39
<211>152
<212>PRT
<213>日本血吸虫(Schistosoma japonicum)
<400>39
MTSETVNIMD LTIPQLQELA RQMEQKIQFI SASIQQLKSL QTQFISSKNY LCELTPDKEN 60
SDILVPLTST LCVPGTLSDA SHIFVDIGTG YYVEMTVLEA ESHFDRRVEY INKQIRKIFP 120
VLEEKTRVHK SISDTLDAKI QDFIKIPPSP CS 152
<210>40
<211>146
<212>PRT
<213>日本血吸虫(Schistosoma japonicum)
<400>40
MTSSFYLRYY VGHKGKFGHE FLEFEFRPEG KLRYANNSNY KNDTMIRKEA YVSPSVMEEL 60
KRIVLESDIM SEDDATWPVP DRVGRQELEI VCGDEHISFT TSKIGSLIDI TNSKDPDGLR 120
TYYYLVQDLK CLVFSLIGLH FKIKPI 146
<210>41
<211>214
<212>PRT
<213>日本血吸虫(Schistosoma japonicum)
<400>41
MLSNGVTSGK MSCADSCPMA EKVRSIIYWR HPIITGVILT LLLIVEFSFM CLSAISFIAY 60
IGLSLLVSVN LVRMYYHFVA KTENNFVREY LEREITVPQD KAEKVSRRLT EVINKGLVRT 120
REIFLLTDPA ASIKFAVLLY LLTYIGAQFN FLTLCILFTF GIFCVPKFYE RYQPEIDKIL 180
ELAKSKIDMV VSQVKSQIEK LPIIGGGGRK EKAQ 214
<210>42
<211>252
<212>PRT
<213>日本血吸虫(Schistosoma japonicum)
<400>42
MPNKQKKVIK AKKIKEIVHH EPDIPVLRKG KASKIAPLPD IAKKTSVKKK VIKNPLIQRR 60
PKNFGIGQNI QPKRNLYRFV KWPKYIELQR KKAILKKRLK IPPPIHQFSQ TLDRNSANQI 120
FNLVTKYQPL SKQAKKMLLV HRAQLRAEGK PDQPTPRKPT VRAGIREYTT AITQKKARLV 180
VIAHDVEPIE IVLFLPALCR KMGVPYCIVK SKAKLGLLLH RKTCTCLAFT NIREEDKSRL 240
SKIVEMGEKQ LQ 252
<210>43
<211>331
<212>PRT
<213>日本血吸虫(Schistosoma japonicum)
<400>43
MGRRKGPKHD HQLKSPNVEH STEELIAESS VSENLTEAPT TKSPDLEVTA CSTETIDVLG 60
NGLVVIKTLR KGLGRETRPS HGDTVVINYK GWLEDGTLVD DVENAKIVLG DGDVIHAFDL 120
SIPLAEHKET FELITDARFA YGSRGRDPDI PSGAKLTYHI EILKVDDPPC YANMPNSERL 180
AIANQKKDRG NYYYRREEFA FAIDSYSKAL KILQLPTSST QSSGEKSDVD CPTELINDAK 240
LKLENNLAAA QLKVEAFDAA IMSCDTVLQS DPQNIKALFR KGKALLEMNE VDDAIPILQK 300
VLTLAPGSQM ASVELARARA IRQKEREHWS R 331
<210>44
<211>176
<212>PRT
<213>日本血吸虫(Schistosoma japonicum)
<400>44
MRANGELKLF SVVGRKLPTT KEPKPVPYRM RIFAPNSVVA KSRFWYFIRK LKKMKKGSGE 60
IISCERIHPR KPLMVKNFGI WLRYDSRSGT HNMYKEYRGL TEESAVTQLY REMGARHRAR 120
AESIQVIKVE AIPASKCRRP YITQFHDSKL KFPLPHRVAR PLHRPRFTTR RPTTAF 176
<210>45
<211>225
<212>PRT
<213>日本血吸虫(Schistosoma japonicum)
<400>45
MPTLSCGYKC LQFLLITFNI LVFACGIALI VIGSLSQVAV NNYSTGIDSN IKGLVIFIII 60
LGCFLFLLGF LGFCGACTKN TCCLTIYAIL LSVMVAAEIG VGIAAAVLKE DVKTSFVSVV 120
RNSVSEYSKN PDIQNLFDKI QTEFKCCGSE SSIDYTSTGQ SVPSSCTDSD TGLAYQEGCS 180
NKIIAFFEKY LIAVVVAAFA FAILQILSIV FTVCIIRAIK SGDTE 225
<210>46
<211>221
<212>PRT
<213>日本血吸虫(Schistosoma japonicum)
<400>46
MKILFICLLL ITLLTMTIQY NLNRKYTNHY RRQPNNPYNR NNNGNNNYDD NNNNDNKNMY 60
DKYSDNSKYG NYYPTNNDYN KQKPKYPRLT RRYSMEGEEK HAHQKHGERH LKSIGELFGF 120
TNTNQGSDYA LTTTFHKGSI AAKNGKQKHF STYETEGQVK HYQNTHGQAE FNLNAKLYGQ 180
EKYNDQKEMK ATVKMEDYDN TPDDEANYSP VGHPYGKLEG Y 221
<210>47
<211>247
<212>PRT
<213>日本血吸虫(Schistosoma japonicum)
<400>47
MVNPIQLMLF GCRSAVWRNS DSNDKGIVVI DDWIPFRMSF TSAARIFAFR PALEALLVRT 60
CLRPEGIADL REEDKRVINV LRQLCSPEAL NPNASFDEAS SYDSPPRKKF ATAGNYPLLS 120
GRSHRTVGYE NSCSEKQGFG RYANCPSRFG VSEQAFDGQC GFWQDPLSQF SNQRYDQSSV 180
NRFPGNYTSI RSFGASGMGN NLNGSSCYSY GVTPFGGYPN QQPQFTNDRF IQQSQRPVNR 240
PHWGIRY 247
<210>48
<211>204
<212>PRT
<213>日本血吸虫(Schistosoma japonicum)
<400>48
MHRSRLCEKR AEYVALIPLT DHRLRPRRIC LKLFGSVVIC SLVFTLLLIF LFPRTIYLRS 60
SVYKLITVSA IANMQDNVID LTVINQINVS NPNFVPAMVN KVIISPKYRS VDLQETVNNS 120
IHMLPLRSET LLNVSTKLRF EHKHFDVIKL CFMKLPYFHD IYVTFTFHIT YMLLWQSFDA 180
YLTIMPLVDC YPTNINSSML MVSS 204
Claims (8)
1.一类日本血吸虫雌性成虫特异蛋白,其特征在于,选自下组:
(a)具有SEQ ID No.25~SEQ ID No.48所示氨基酸序列的蛋白;
(b)由(a)的蛋白发生一个或几个氨基酸置换、缺失或***而形成的具有(a)蛋白相同活性的蛋白。
2.如权利要求1所述的蛋白,其特征在于,它具有SEQ ID No.25~SEQ ID No.48所示的氨基酸序列。
3.一类日本血吸虫雌性成虫特异蛋白基因,其特征在于,它编码权利要求1所述的蛋白。
4.如权利要求3所述的基因,其特征在于,选自下组:
(a)SEQ ID No.1~SEQ ID No.24所示的核苷酸序列;
(b)SEQ ID No.1~SEQ ID No.24所示的核苷酸序列中每条序列的互补序列;
(c)与SEQ ID No.1~SEQ ID No.24所示的核苷酸序列中每条序列有至少70%同源性、且编码相同功能蛋白质的核苷酸序列。
5.如权利要求3或4所述的基因,其特征在于,它具有SEQ ID No.1~SEQ ID No.24所示的核苷酸序列。
6.一类日本血吸虫病防治疫苗,其特征在于,所述疫苗以权利要求1所述的蛋白为免疫原。
7.一类能与权利要求1所述的蛋白特异性结合的抗体。
8.权利要求1所述的蛋白在制备预防和治疗日本血吸虫病的药物中的应用。
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102167735A (zh) * | 2011-03-21 | 2011-08-31 | 中国疾病预防控制中心寄生虫病预防控制所 | 日本血吸虫SjTollip重组抗原蛋白及其制备方法和用途 |
CN101597645B (zh) * | 2009-06-26 | 2012-05-30 | 华南农业大学 | 一种日本血吸虫性别特异性检测引物、检测试剂盒和检测方法 |
CN115961051A (zh) * | 2019-06-18 | 2023-04-14 | 中国医学科学院病原生物学研究所 | 日本血吸虫w染色体特异基因的筛选及其在尾蚴性别鉴定中的应用 |
-
2005
- 2005-12-20 CN CNA2005101117348A patent/CN1986567A/zh active Pending
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101597645B (zh) * | 2009-06-26 | 2012-05-30 | 华南农业大学 | 一种日本血吸虫性别特异性检测引物、检测试剂盒和检测方法 |
CN102167735A (zh) * | 2011-03-21 | 2011-08-31 | 中国疾病预防控制中心寄生虫病预防控制所 | 日本血吸虫SjTollip重组抗原蛋白及其制备方法和用途 |
CN115961051A (zh) * | 2019-06-18 | 2023-04-14 | 中国医学科学院病原生物学研究所 | 日本血吸虫w染色体特异基因的筛选及其在尾蚴性别鉴定中的应用 |
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