CN1913910B - Composition comprising hovenia dulcis thunb. extract, lindera obtusiloba blume extract, or herbal mixture extract thereof - Google Patents
Composition comprising hovenia dulcis thunb. extract, lindera obtusiloba blume extract, or herbal mixture extract thereof Download PDFInfo
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Abstract
The present invention relates to a composition comprising Hovenia dulcis Thunb extract, Lindera obtusiloba extract or herbal mixture extract thereof as an effective ingredient. Hovenia dulcis Thunb extract, Lindera obtusiloba extract or herbal mixture extract thereof of the present invention is highly effective for the improvement of liver functions since it can lower the level of GOT, GPT, ALP, BUN and total bilirubin, which are major liver function indices. ALP and BUN are also used as kidney function indices, so the decrease of the level of ALP and BUN by the extract of the present invention indicates that Hovenia dulcis Thunb extract, Lindera obtusiloba extract or herbal mixture extract thereof of the present invention can improve kidney functions as well. Hovenia dulcis Thunb extract, Lindera obtusiloba extract or herbal mixture extract thereof of the present invention also can lower the amount of hydroxyproline in liver but increase the amount of hydroxyproline in kidney, suggesting that the extract above has excellent anti-fibrosis and kidney protecting effects. In addition, Hovenia dulcis Thunb extract, Lindera obtusiloba extract or herbal mixture extract thereof of the present invention can lower the level of malondialdehyde, an index of lipid peroxidation in liver and kidney tissues, suggesting that the extract has excellent anti-oxidative effect. Hovenia dulcis Thunb extract, Lindera obtusiloba extract or herbal mixture extract thereof of the present invention promotes cell viability in liver and kidney cell lines, indicating that the extract has excellent liver and kidney cell protective effects. Therefore, the composition of the present invention can be effectively used not only for anti-oxidation and anti-fibrosis but also for the protection and the improvement of liver and kidney functions.
Description
[technical field]
The present invention relates to comprise Semen Hoveniae (Hovenia dulcis Thunb) extract, Cortex Linderae Obtusilobae (Lindera obtusiloba) extract or its herbal mixture extract are as the compositions of effective ingredient.
[background field]
As one of representational adult disease, the hepatic injury that the confirmed fatigue that hepatopathy is caused by pressure and most of allogenic material are caused causes.Compare with foreign country, very high in the development rate of Korea S's hepatopathy, and especially, liver cancer mortality is the highest in the world, the mortality rate of chronic hepatopathy occupy the 3rd.According to the nearest report of Korea S State Statistics Bureau (National Statistical Office), hepatopathy is the adult's main causes of death that cause 40 years old age bracket of Korea S.In Korea S, fatal diseases is a viral hepatitis in many hepatopathys.Simultaneously, in western countries, dead 5～10 times that are caused by liver cirrhosis are higher than the death that is caused by viral hepatitis.
Liver is to show to enliven metabolic organ most in many human organs.By multiple reason such as the fatty diet, severe excessive drinking, viral infection comprises the toxin of chemicals, the acute or chronic hepatic injury that undernutrition etc. cause can form serious disease such as fatty liver, hepatitis, jaundice, liver cirrhosis and hepatocarcinoma.Especially, fatty diet and severe excessive drinking cause fatty liver, and promptly fat is accumulated in the hepatic tissue.At this moment, GOT in the serum (glutamic-oxaloacetic transaminase), GPT (glutamic acid-acetone acid transaminase) and γ-GTP (gamma glutamyl transpeptidase) increase.Because liver has huge buffer capacity, hepatopathy shows symptom in early days hardly, and and if only if it when becoming serious, just shows symptom.
Liver has keeping of blood and circulation, the adjusting of blood flow and the function of detoxification, and the known ergasia that relates to.Because industrialization, human body is exposed to and pollutes and many toxin, thereby so liver must excessively work and neutralize a toxin.Bad more by the hepatic injury that stress causes.If people's spirit is loosened, the hepatocyte of damage is recovered.For modern resident, situation is not have the unnecessary time to be used for having a rest.Therefore, over-drastic stress, indulge in cigarette and excessive drinking worsen hepatic injury, cause the malfunction of detoxification, and promptly functions of immune system is not normal, and this may be the reason of another kind of disease.
Liver cirrhosis by hepatic tissue fibre modification caused, this is a unbalanced disease in the generation of connective tissue and between degenerating.That is, connective tissue is excessively accumulation in hepatic tissue, follows necrosis or inflammation.The fibre modification of hepatic tissue is propulsive by the excessive generation institute of connective tissue, this is because the growth of hepatic stellate cell (HSCs) and transfer institute cause, described hepatic stellate cell is with the form of myofibroblast (the Gressner et al. that is transformed, Biochem.Biophys.Res.Commun.151～222,1988).Liver cirrhosis is the terminal stage of every kind of chronic hepatopathy, loses its general function rapidly by its liver, is shown as the minimizing of liver blood stream, the minimizing of blood flow in the liver, the malfunction of metabolic enzyme, the qualitative and quantitative variation of hematoglobin protein, the effusive variation of bile etc.
The hepatotoxicity inducer is illustrated as carbon tetrachloride (CCl
4), D-galactosamine, lipopolysaccharide (LPS) and bromobenzene, and especially, known carbon tetrachloride is induced hepatotoxicity (Recknagel et al., Pharmacol.Rev.19:145-208,1967 by the lipid peroxidation of inducing hepatocyte; Alpers et al., Mol.Pharmacol.4:566-573,1968; Slater T.F., Biochem.J.222:1～15,1994).Carbon tetrachloride is the hepatotoxicity inducer, and it has been used for studying anti-hepatotoxicity activity.More precisely, it has been applied to mice or rat, the hepatocyte of cultivation and the hepatic tissue of cultivation are with artificial induction's hepatotoxicity.In endoplasmic reticulum, carbon tetrachloride is converted into free radical CCl by metabolic enzyme such as Cytochrome P450
3The high activity molecular structure, by it, it needs very strong hepatotoxicity effect.Free radical CCl
3Triglyceride that accumulates in liver by pure oxidation and the fatty acid on membrane phospholipid have caused the acidify and the oxidation of lipid, carry out lipid peroxidation subsequently.As a result, produced organic peroxide.By such lipid peroxidation, fat accumulates in liver, and protein synthesis reduces, glycogenolysis, and the Cytoplasm enzyme in the blood vessel is destroyed, has caused necrosis (Chang I.M., the et al. of hepatic tissue, Drug and chemicaltoxicology 6,443-453,1983).Free radical has also damaged Golgi body, thereby the protein that discharges cell is affected, and points out not only liver, and kidney also might sustain damage.
Antioxidation not only suppresses or has prevented lipid peroxidation, also has liver protection and antiphlogistic effects.Therefore, anti-oxidizing compounds has been used for protecting liver and hepatocyte at the attack of reactive oxygen intermediate.
Reported that isolating antioxidant is such as flavone compound from natural origin, quercetin, silymarin or vitamin E have for the fibrotic good effect of lipid peroxidation regulating liver-QI.And, confirmed that N-acetylcysteine (NAC) has reduced the oxidative pressure and the hepatic fibrosis of the commitment of hepatic fibrosis by its antioxidant activity.Picrorhiza Kurroa (picroside) is another kind of natural anti-oxidation chemical compound, and it has liver protection and fibrosis effect by reducing the damage that is caused by lipid peroxidation and free radical.
Up to the present, the Therapeutic Method that will be used for hepatopathy mainly is divided into dual mode; A kind of mode is a Diet Therapy, and another kind of mode is a Drug therapy.In most applications, two kinds of methods are used together.For Drug therapy,, use various medicines with difference in functionality according to the cause and the type of hepatopathy.For example, use hepatocyte to duplicate stimulus object and/or liver function-protecting such as ursodesoxycholic acid, silymarin (Biotech.Therapeutics, 4,263-270,1993), DDB (diphenyl dimethyl dicarboxylate (ester); Biochem.Biophy.Res.Comm., 103,1131-1137,1981), glutathion, glycyrrhizin etc., antiviral agent such as acyclovir and immunosuppressive drug be such as corticosteroid, Ismipur (6-MP), azathioprine etc.Yet hepatopathy is not only by a reason but developed by the combined effect of many factors.Therefore, a kind of medicine with certain function is not enough to treat various hepatopathys.The conventional medicine of treatment hepatopathy is by using the problem with the reaction that can't expect and other side effect for a long time or in a large number.
Simultaneously, kidney has the concentration of regulating ion and body fluid, discharges refuse (urine, uric acid, creatinine etc.) and remove toxicant, the function of medicine and metabolite after eliminating toxin from system.The known product that comes lipid peroxidation in the self-organizing, malonaldehyde (Malonedialdehyde) (MDA) and 4-hydroxynonenal (HNE) be the index of cell injury.And the superoxide dismutase of known catalysis lipid peroxidation relates to recovery (Slater TF.Biochem.J., 222,1-15,1994 from cell injury; Esterbauer H, Schauer RJ, Zollner H., 1994; Free RadicalBiology﹠amp; Medicine 11,81-128,1992).In addition, also with alkali phosphatase (ALP) and hematuria nitrogen (BUN) diagnosis index as renal function.Especially, the increase of BUN indication is by the minimizing of azotemia glomerular filtration rate (GFR).Azotemia means the dysfunction of kidney before the kidney, and does not have injury of kidney, and this is because congestive heart failure, shock, hypoglycemia and the hemorrhage hypoperfusion that causes cause.On the other hand, azotemia is to cause when urine stream is blocked in the bottom of kidney behind the kidney.In the situation of azotemia, expection reaches rehabilitation completely by eliminating cause behind kidney.When azotemia is followed various clinical symptoms and biochemical disorder, be referred to as uremia.Therefore, uremia is not only single biochemical disorder, still carries the syndrome of metabolic disease, endocrinopathy, gastroenteropathy, neuromuscular disease and cardiovascular disease except eliminate a disease (elimination disorders).The disease that dysfunction caused by kidney is illustrated as acute pyelonephritis, chronic pyelonephritis, renal tuberculosis, UTI, urinary stone, nephrocyte cancer etc.
Semen Hoveniae is a kind of tall and big trees of rhamnaceous broad leaved and deciduous broad leaved that belong to, and it is also referred to as ' Jigumok ' in Chinese medicine.Relevant for the explanation of Semen Hoveniae, its flavor is sweet and pure, nontoxic in Bon-cho-kang-mok, make the five internal organs (heart, liver, spleen, lung and kidney) working stability and mild, eliminate the heating in the thoracic cavity, suppress thirsty, in and alcoholism, slow down vomiting, separate insecticide poison and can treat five types hemorrhoid.Also known its has liver protection effect.Say that exactly it has the elimination halitosis, improve alcoholic hepatitis, fatty liver and liver cirrhosis, anticancer, blood pressure regulation, blood sugar lowering, the excellent activity of liver detoxifcation and constipation relieving.
Cortex Linderae Obtusilobae is a kind of tall and big trees of broad leaved and deciduous broad leaved that belong to Lauraceae, and it extensively is distributed in Korea S, Japan, China and Manchuria.The flower of Cortex Linderae Obtusilobae, leaf and stem give out unique fragrance.Because their antibacterial action is used for medicament purpose with its stem.The bud that it is fresh is as tea.
Behind the natural drug chemical compound that studied for a long period of time with various mechanism of action and low toxicity; the inventor is by confirming the Semen Hoveniae extract; Cortex Linderae Obtusilobae extract or its herbal mixture extract are except protection and improve the activity of renal function, also have antioxidation, fibrosis and improve the activity of liver function and finished the present invention.
[summary of the invention]
[technical scheme]
A target of the present invention provides and comprises the Semen Hoveniae extract, and Cortex Linderae Obtusilobae extract or its herbal mixture extract are as the compositions of effective ingredient.
[best mode]
The invention provides and comprise the Semen Hoveniae extract, Cortex Linderae Obtusilobae extract or its herbal mixture extract are as the chemical compound of effective ingredient.
Compositions of the present invention comprises antioxidant composition, and fibrosis compositions, liver function improve compositions and renal function improves compositions.
Hereinafter, will describe the present invention in detail.
Semen Hoveniae extract of the present invention, the extracting method of Cortex Linderae Obtusilobae extract or its herbal mixture extract is as follows.
Wash Semen Hoveniae or Cortex Linderae Obtusilobae with water, follow it in the shady place drying.Exsiccant Semen Hoveniae or Cortex Linderae Obtusilobae are placed the reflection extractor,, obtained the hot water extract to the water that wherein adds purification and 100 ℃ of heating 90 minutes.When it still is heat, under reduced pressure the hot water extract is filtered with filter paper.Leach thing by using vacuum evaporator to concentrate.For long term store, come drying solution by using freeze dryer.In the present invention, the stem of Semen Hoveniae or Cortex Linderae Obtusilobae, flower, leaf and seed all are available.
Determine the combination ratio of the book on Chinese herbal medicine of compositions of the present invention based on the dry weight of every kind of book on Chinese herbal medicine.Say exactly, Semen Hoveniae and the Cortex Linderae Obtusilobae ratio with 3: 2～1: 1 is mixed, more preferably mix with 2: 1～1: 1 ratio.Determine such ratio under the effective dose of considering every kind of book on Chinese herbal medicine and side effect thereof, when ratio was not in above-mentioned scope, effect of drugs descended rapidly or side effect may become problem.
Using the Semen Hoveniae extract, in the group of Cortex Linderae Obtusilobae extract or its herbal mixture extract, all as the GOT of liver function index, GPT, ALP, BUN and total bilirubin have all descended, and illustrate that extract of the present invention has splendid liver function improvement effect.Simultaneously, not only ALP and BUN are used as liver function index, also used as renal function index.Therefore, the level of the reduction of ALP and BUN illustrates Semen Hoveniae extract of the present invention, and Cortex Linderae Obtusilobae extract or its herbal mixture extract also have splendid renal function and improve effect.
Semen Hoveniae extract of the present invention; Cortex Linderae Obtusilobae extract or its herbal mixture extract have reduced the amount of the hydroxyproline of hepatic tissue; but increased the amount of the hydroxyproline in the nephridial tissue, illustrated that extract of the present invention has splendid fibrosis and kidney protective effect.Especially, extraction shows than every kind of effect that independent extract is higher from the herbal extract of the mixture of Semen Hoveniae and Cortex Linderae Obtusilobae, promptly, compare with every kind of independent extract, mixture extract reduces hydroxyproline more in hepatic tissue, but increases hydroxyproline in nephridial tissue more.
Semen Hoveniae extract of the present invention, Cortex Linderae Obtusilobae extract or its herbal mixture extract can reduce the level as the malonaldehyde of lipid peroxidation index in liver and the nephridial tissue, illustrate that extract of the present invention has splendid antioxidation.
With Semen Hoveniae extract of the present invention, hepatic cell line and kidney cell line that Cortex Linderae Obtusilobae extract or its herbal mixture extract are handled show high cell survival, illustrate that extract of the present invention has splendid hepatocyte and nephrocyte protective effect.
Therefore, compositions of the present invention can be used for improving and protection liver and renal function and be used for antioxidation and fibrosis effectively.
Except the Semen Hoveniae extract, outside Cortex Linderae Obtusilobae extract or its herbal mixture extract, compositions of the present invention can comprise in addition, one or more effective ingredient that have same or similar function with extract of the present invention.
Semen Hoveniae extract of the present invention, Cortex Linderae Obtusilobae extract or its herbal mixture extract can be oral or parenteral administration and can using with the general type of pharmaceutical preparation.Herbal mixture extract of the present invention can be by mixing with filler, supplement, binding agent, wetting agent commonly used, and disintegrating agent, diluent be such as surfactant, or excipient is prepared and is used for oral or parenteral administration.Being used for Orally administered solid preparation is tablet, pill, face powder, granule and capsule.By mixing one or more excipient that are fit to such as starch, calcium carbonate, sucrose, lactose, gelatin wait and prepare these solid preparations.Except simple excipient, can make with lubricator, for example magnesium stearate, Talcum, etc.Being used for Orally administered liquid preparation is that suspension, solution, Emulsion and syrup and the above-mentioned preparation of mentioning can comprise various excipient such as wetting agent, sweeting agent, aromatic and antiseptic, and simple diluent such as water and liquid paraffin commonly used.The preparation that is used for parenteral administration is aseptic aqueous solution, the excipient that is insoluble in water, suspension, Emulsion, lyophilized preparation and suppository.Be insoluble in the excipient of water and suspension except active a kind of chemical compound or multiple chemical compound, can comprise propylene glycol, Polyethylene Glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate etc.Except active one or more chemical compounds, suppository can comprise witepsol, Polyethylene Glycol, tween 61, cocoa butter, laurin fat, glycerol, gelatin etc.
Dosage unit for example can comprise, 1,2,3 or 4 individually dosed or 1/2,1/3 or 1/4 independent dosage.Independent dosage is preferably included in the amount of active compound administered in the applied once and its usually corresponding to complete, 1/2,1/3 or 1/4 dosage every day.Semen Hoveniae extract of the present invention, the effective dose of Cortex Linderae Obtusilobae extract or its herbal mixture extract are respectively 200～600mg/kg, and more preferably 300～400mg/kg, and application times is every day 1～6 time.
Thereby compositions of the present invention can be improved liver and renal function and obtain antioxidation and the fibrosis effect with surgical operation, radiotherapy, hormone therapy, chemotherapy and biological response modifier combined administration alone or in combination.
Improve liver and renal function in the health food and obtain antioxidation and the fibrosis effect thereby compositions of the present invention can be added on.At this moment, can be according to conventional method with Semen Hoveniae extract of the present invention, Cortex Linderae Obtusilobae extract or its herbal mixture extract former state add or mix to add behind other food or the composition.Determine the blending ratio of effective ingredient according to the purpose of using (prevention, health or therapeutic treatment).Comprise Semen Hoveniae extract of the present invention in production, in the situation of the Foods or drinks of Cortex Linderae Obtusilobae extract or its herbal mixture extract, preferably with described extract with under the raw-material 15 weight %, more preferably 10 weight % add down.Yet, when its chronic administration when improving health status, the content of extract can be lower than above-mentioned, but effective dose can comprise and surpasses above-mentioned amount because extract of the present invention is as safe as a house.
Do not have restriction in applicable food, it is illustrated as meat, sausage, bread, chocolate, confection, snack, cookies, pizza, ramyun, noodles, chewing gum, milk product and comprises that ice cream, soup, beverage, tea, wine, alcoholic beverage and vitamin complex etc. and every kind of health food in fact will generally producing are all included.
Comprise the healthy beverage of compositions of the present invention such as other beverage, can comprise various food flavors or natural sugar etc. in addition.Above-mentioned natural sugar can be monosaccharide such as glucose and fructose, disaccharide such as maltose and sucrose, polysaccharide such as dextrin and cyclodextrin and sugar alcohol such as xilytole, Sorbitol and erythritol one of them.As sweeting agent, can use natural sweetener such as thaumatin and stevia extract or artificial sweetening agent such as glucide and radix asparagi sweet extract.The ratio of natural sugar and compositions of the present invention preferably 0.01～0.04g more preferably is that 0.02～0.03g is to 100ml to 100ml.
Except the above-mentioned composition of mentioning, compositions of the present invention can comprise multiple nutritional components, vitamin, electrolyte, fumet, coloring agent, pectic acid and salt thereof, arginine (arginic acid) and salt thereof, organic acid, protective colloid viscosifying agent (viscosifiers), pH regulator agent, stabilizing agent, antiseptic, glycerol, alcohol, be used to add the carbonating agent (carbonators) of soda etc.Compositions of the present invention can also comprise natural fruit juice, fruit nectar and/or can add the plant beverage pulp.All compositions of mentioning can add separately or together.In fact the mixed ratio of those compositions is unimportant, but common every kind can be with 100 wt parts of 0.01～0.1 wt part/compositions of the present invention.
[invention mode]
Practice of the present invention and present embodiment preferred are illustrated as in the following embodiments.
Yet, will be appreciated that those skilled in the art, after having considered this description, can modify and improve within the spirit and scope of the present invention.
＜embodiment 1〉preparation of Semen Hoveniae extract
Wash Semen Hoveniae with water, follow it in the shady place drying.The exsiccant Semen Hoveniae of 20g is placed the reflection extractor, to the water that wherein adds the 1.5l purification and 100 ℃ of heating 90 minutes.When the hot water extract still is heat, under reduced pressure it is filtered with filter paper.Leach thing by using vacuum evaporator to concentrate.It is carried out drying to be used for long term store by freeze dryer.
Spissated solution is used for animal testing (2ml/ rat/sky).
＜embodiment 2〉preparation of Cortex Linderae Obtusilobae extract
By using Cortex Linderae Obtusilobae, the Cortex Linderae Obtusilobae extract is prepared in the method described in the embodiment 1 to be similar to as above-mentioned.Spissated solution is used for animal testing (2ml/ rat/sky).
＜embodiment 3〉preparation of herbal mixture extract
The dry Cortex Linderae Obtusilobae stem of the dry Semen Hoveniae of 20g and 10g is mixed and prepare its extract to be similar to the method described in the embodiment 1.
Spissated solution is used for animal testing (2ml/ rat/sky), and 4 times of spissated solution are used for MTT and NR mensuration (50 μ l/ hole).
＜EXPERIMENTAL EXAMPLE 1〉antioxidation, fibrosis, liver and renal function improvement and the protective effect of extract in rat, described rat has inductive liver and injury of kidney by the chronic administration carbon tetrachloride
Thereby following experimentizing studied Semen Hoveniae extract of the present invention, antioxidation and fibrosis effect and liver and renal function improvement and the protective effect in liver and renal injury model of Cortex Linderae Obtusilobae extract or its herbal mixture extract.
1. test animal
Be weighed as about 180～210g 12 age in week the Sprague-Dawley rat (Damul science, Osan is Korea) as test animal.Raising temperature is that 23 ± 2 ℃ and relative humidity are maintained 60 ± 10%.Arbitrarily supply with feedstuff (Purina feedstuff) and water, and regulate the cycle at day-night.
Make 2 weeks of animal adaptive testing chamber, and then they are divided into 5 groups, 1. normal group, 2. CCl
4Processed group, 3. CCl
4+ herbal mixture extract processed group, 4. CCl
4+ Semen Hoveniae extract-treated groups and 5. CCl
4+ Cortex Linderae Obtusilobae extract-treated groups.Each group is made up of 10 rats.2. hepatic fibrosis (sclerosis) and injury of kidney induces
Except normal group, with 1ml/ rat/day, with olive oil and CCl
4Mixture is used to experimental group, thereby 3 lasting 4 weeks an of week are induced hepatic fibrosis (sclerosis) and injury of kidney.
With 2ml/ rat/day, to CCl
4The Orally administered distilled water of processed group, with 2ml/ rat/day to CCl
4+ herbal mixture extract processed group Orally administered in the foregoing description 3 preparation herbal mixture extract, with 2ml/ rat/day to CCl
4+ Semen Hoveniae extract-treated groups Orally administered in the foregoing description 1 preparation the Semen Hoveniae extract and with 2ml/ rat/day, to CCl
4The Cortex Linderae Obtusilobae extract of the Orally administered preparation in the foregoing description 2 of+Cortex Linderae Obtusilobae extract-treated groups.
To weigh the rat in comprising every group of normal group, and then it be used etherization.By cardiocentesis,, place room temperature to reach described blood above 2 hours with blood extraction from heart.Then, carry out centrifugally reaching 10 minutes, described serum is stored in-20 ℃ to obtain serum at 3000rpm.Check that serum is to measure GOT, GPT, alkali phosphatase, the level of BUN and total bilirubin.
Extract liver and the kidney of rat, it with phosphate buffer (pH 7.0) washing, is measured its weight subsequently, described liver and kidney are because the artificial induction has been subjected to damage.The part of liver and nephridial tissue is kept at-75 ℃ to be applied to measure hydroxyproline and malonaldehyde (MDA).
Measure weight change weekly.Research ear, tail and foot detect the sign of jaundice.When rat is put to death, liver is weighed.
The result is represented by meansigma methods ± SD, and check the significance of the difference of checking the meansigma methods between matched group and the experimental group by Si Shi t-.When p＜0.005, it is judged as the significance that has on the statistics.
To the results are shown in table 1
[table 1]
*：p＜0.005
As shown at table 1, compared with the control, with Semen Hoveniae extract of the present invention, body weight is inapparent with the variation of the ratio of liver weight in the experimental group that Cortex Linderae Obtusilobae extract and herbal mixture extract are handled.Equally, compared with the control, the variation of the ratio of body weight and kidney weight also is inapparent.
3. serology and biochemical test
1) GOT (AST) measures (using the EMBIEL test kit)
The AST substrate solution of 500 μ l is placed two falcon pipes, it was heated 3～5 minutes at 37 ℃.In a pipe, substrate solution is diluted with standard solution, and in another pipe, add the blood serum sample of 100 μ l.In 37 ℃, induced reaction reaches 60 minutes in two pipes.The water of the purification of 100 μ l and the coloring solution (2,3-dinitrophenyl hydrazine) of 500 μ l are added in each pipe, then be placed on room temperature and reach 20 minutes.The 0.4N NaOH of 5ml is added in each pipe, subsequently room temperature reaction 10 minutes.Measure OD at 505nm.
2) GPT (ALT) measures (using the EMBIEL test kit)
The ALT substrate solution of 150 μ l is placed two falcon pipes, it was heated 4 minutes at 37 ℃.In a pipe, substrate solution is diluted with standard solution, and in another pipe, add the blood serum sample of 100 μ l and in 37 ℃, induced reaction reaches 30 minutes in two pipes.The water of the purification of 100 μ l and the coloring solution (2,3-dinitrophenyl hydrazine) of 500 μ l are added in each pipe, then be placed on room temperature and reach 20 minutes.The 0.4N NaOH of 1.5ml is added in each testing tube, subsequently room temperature reaction 10 minutes.Measure OD at 505nm.
3) ALP (alkali phosphatase) measures
The ALP substrate buffer solution (2-phosphenylic acid sodium) of 2.0ml is placed three testing tubes, it was reacted 3～5 minutes at 37 ℃.With the blood serum sample of 50 μ l, the standard solution of the water of the purification of 50 μ l and 50 μ l adds respectively in the testing tube, subsequently 37 ℃ of reactions 15 minutes.The coloring agent of 2.0ml is added in each testing tube, and then be placed on room temperature and reach 10 minutes.In 60 minutes, measure OD at 570nm.
4) BUN (blood urinary nitrogen) measures
With the blood serum sample of 20 μ l, the standard solution of the water of the purification of 20 μ l and 20 μ l places three testing tubes respectively.The enzymatic solution of 2.0ml is added wherein, subsequently 37 ℃ of reactions 5 minutes.The coloring agent of 2.0ml is added in each pipe, it was heated 10 minutes at 37 ℃.In 60 minutes, measure OD in 580nm.
5) measurement of total bilirubin
With the blood serum sample of 100 μ l, the standard solution of the water of the purification of 100 μ l and 100 μ l places and places three testing tubes respectively, with 600 μ l/ pipes to wherein adding the coloring agent that works at total bilirubin.The diazonium mixture of 600 μ l is added in each testing tube, be placed on room temperature and reach 10 minutes.The ferring reagent of 600 μ l is added in each pipe with induced reaction.In 2 hours, measure OD in 600nm.
To the results are shown in table 2
[table 2]
| GOT(IU) | GPT(IU) | ALP(KA) | BUN (mg/□) | Total bilirubin (mg/) | |
| Normal group | 61.78±6.0 | 13.2± 1.0 | 63.5± 11.2 | 13.6± 1.1 | 0.2±0.05 |
| CCl 4Processed group | 241.8±43.1 | 145.3±38.1 | 156.9±36.1 | 23.4± 2.1 | 1.6±0.92 |
| CCl 4+ herbal mixture extract processed group | 137.6±52.4 * * | 69.6±47.0 * | 137.0±26.0 | 1g.04±6.9 1 | 0.27±0.14 |
| CCl 4+ Semen Hoveniae extract-treated groups | 165.1±46.4 * * | 70.9±42.2 * | 143.1±37.9 | 18.6± 2.1 * | 0.7±0.04 |
| CCl 4+ Cortex Linderae Obtusilobae extract-treated groups | 144.5±5.7 ** | 74.4±17.6 * | 138.2±14.8 | 18.4± 3.6 | - |
*：p＜0.05，
**：p＜0.005
As be displayed in Table 2, compared with the control, using Semen Hoveniae extract of the present invention respectively, in the experimental group that Cortex Linderae Obtusilobae extract and its herbal mixture extract are handled, as the exponential GOT of liver function, GPT, ALP, the level of BUN and total bilirubin is significantly lower.Especially, in the group of handling with herbal mixture extract, those exponential levels are minimum.
Therefore, prove Semen Hoveniae extract of the present invention, Cortex Linderae Obtusilobae extract and its herbal mixture extract have splendid liver function improvement effect.
And, ALP and BUN are not only the index that is used for liver function, it also is the index that is used for renal function, therefore, the level of ALP that extract of the present invention causes and the reduction of BUN also means Semen Hoveniae extract of the present invention, and Cortex Linderae Obtusilobae extract and its herbal mixture extract also have splendid renal function improvement effect.
4. the measurement of hydroxyproline (hyp)
1) preparation of reagent
1. acetate citrate buffer
With the sodium acetate trihydrate of 50g, the trisodium citrate of 37.5g and the monohydrate potassium of 5.5g are mixed in the isopropyl alcohol of 395ml.And, the cumulative volume of mixture is adjusted to 1L by adding distilled water.Also the pH with mixed solution adjusts to 6.0.Final solution is stored in 4 ℃.
2. chloramine-T solution
The chloramine-T of 84mg is dissolved in the acetate citrate buffer of 10ml.
3. Ehrilich ' s reagent
60% the perchloric acid of the right-dimethylamino benzaldehyde of mixed 10g and 11ml is with the preparation stock solution.The stock solution of 3ml is mixed isopropyl alcohol with 8.0ml, obtain Ehrilich ' s reagent.Prepare described reagent before use immediately, and this stock solution is stored in 4 ℃ at shady place.
2) measurement of hydroxyproline
Liver (or kidney) tissue of the 0.2g of freezing or lyophilizing (50 ℃, 72 hours) is dispersed in the vial of 10ml.The 6N HCl of 4ml is placed this bottle, carry out homogenate with homogenizer subsequently.Then, being hydrolyzed in 110 ℃ in exsiccant baking oven reaches 10～24 hours, filters with Whatmann filter paper subsequently.Equally, will be with trans-hydroxy proline 6N HCl dilution, 0,0.2, the standard solution of the variable concentrations of 0.4,0.6,0.8 and 1.0 μ g/50 μ l is also 110 ℃ of hydrolysis 12～14 hours.Each gets 50 μ l with every kind of sample and standard solution, and in vial finish-drying to remove HCl.50% isopropyl alcohol that adds 1.2ml comes the dissolution precipitation thing.The chloramine-T solution of 200 μ l is added wherein, subsequently room temperature reaction 10 minutes.Then, Ehrilich ' the s reagent that adds 1.2ml.Induced painted 90 minutes at 50 ℃, cool off in room temperature subsequently.Measure OD by using spectrophotometer at 558nm.
Measure the content of hydroxyproline in the hepatic tissue (or nephridial tissue) by following formula.
The hydroxyproline concentration of C[0.2g hepatic tissue (or nephridial tissue)]=
[HA (OD of sample)/SA (with the OD of the standard solution of the trans hydroxyproline 6N of 1.0 μ g/50 μ l HCl dilution)] * 80
C * 5=hydroxyproline amount/g hepatic tissue (or nephridial tissue)
The results are shown in the table 3.
[table 3]
*：p＜0.005
As shown in table 3, compared with the control, at each personal Semen Hoveniae extract of the present invention, in the experimental group that Cortex Linderae Obtusilobae extract and herbal mixture extract thereof are handled, the hydroxyproline content in the hepatic tissue is lower and higher in nephridial tissue.Especially, compared with the control, in the group of handling with herbal mixture extract, the hydroxyproline content in hepatic tissue significantly low (50.8%).But the comparison of the hydroxyproline content in kidney is according to high by 5.5%, than high by 3.0% with the group of Semen Hoveniae extract-treated, than high by 1.2% with the group of Cortex Linderae Obtusilobae extract-treated.
Thereby, having confirmed Semen Hoveniae extract of the present invention, Cortex Linderae Obtusilobae extract and herbal mixture extract thereof have fabulous fibrosis and kidney protective effect.
The measurement of (5.MDA malonaldehyde)
The tissue sample [the 0.2g hepatic tissue (or nephridial tissue) in the 1.15%KCl of 1.8ml] of homogenate and the standard substance (0,4,8,16,32nmol/200 μ l tetramethoxy propane) of 200 μ l dilution are put together in the falcon pipe.200 μ l are mixed with the 0.2%SDS of 100 μ l mutually with the standard solution of diluted sample, subsequently in room temperature reaction 10 minutes.To 20% acetic acid that wherein adds 750 μ l and 750 μ l, 0.8% Thiobarbiturate.Solution in 95 ℃ of reactions 30 minutes, is cooled off in ice subsequently.After finishing cooling, add the n-butyl alcohol of 2ml, carry out centrifugal subsequently to obtain supernatant.On 532nm, measure the OD of supernatant by spectrophotometer.
The content of MDA in following measurement serum and the tissue.
The concentration of malondialdehyde of C[0.2g hepatic tissue (or nephridial tissue) (or 200 μ l serum)]=[HA (OD of sample)/SA (OD of standard solution (8 μ mol/1.15%KCl, 200 μ l)) * 80
C * 5=content of MDA (μ mol/ml)
The result is presented in the table 4.
[table 4]
*：p＜0.05
As shown in table 4, compared with the control, at each personal Semen Hoveniae extract of the present invention, in the experimental group that Cortex Linderae Obtusilobae extract and herbal mixture extract thereof are handled, the amount of malonaldehyde, the index of lipid peroxidation is low in liver and nephridial tissue.Especially, in the group of handling with herbal mixture extract, compared with the control, the content in hepatic tissue significantly low (31.0%) and content also very low (13.0%) in the nephridial tissue.
Therefore, confirmed Semen Hoveniae extract of the present invention, Cortex Linderae Obtusilobae extract and herbal mixture extract thereof have fabulous non-oxidizability and kidney protective effect.
＜EXPERIMENTAL EXAMPLE 2〉the cytotoxicity test
Used cell line is NCTC clone's 1469 (hepatic cell lines) and vero (kidney cell line) among the present invention, and it available from Korea S cell line storehouse (Korea Cell Line Bank) (KCLB).
5mg MTT powder is dissolved in the 1ml phosphate buffer, it is fully stirred and filter by 0.4 μ m filter subsequently, obtain the MTT stock solution.
4mg NR powder is dissolved in the triple distillation water of 1ml, then it is fully stirred and filter, obtain the NR stock solution by 0.4 μ m filter.
Purchase contains trypsin-EDTA of 0.5% trypsin and 5.3mM EDTA and carries out 10 times of dilutions with PBS before use.
1) MTT[3-(4,5-dimethylthiazole-2-yl)-2,5-brominated diphenyl base tetrazolium salts] measure
After removing culture medium, add 1ml trypsin-EDTA solution, it was placed 10～20 minutes.Isolated cell and transferring to subsequently in the falcon pipe of 15ml from container.Join the 1ml culture medium in the culture bottle and shake, it is also placed the falcon pipe to separate all remaining cells.With the cell suspension of 10 μ l place hematimeter and subsequently pair cell count (2.5 * 10
4Cell/ml).With 50 μ l (1～2 * 10
5Cell)/hole seeds cells on 96 orifice plates, adds the culture medium (DMEM+10%FBS+ antibiotic) of 150 μ l to it.With cell culture in 37 ℃, 5%CO
2In the incubator 24 hours, cause cell attachment.
After 24 hours, culture medium is removed the cell on being attached to 96 orifice plates carefully (need careful note making culture medium not to be infected with and to pollute) by cell.The culture medium of 150 μ l is added the Semen Hoveniae extract for preparing with among the embodiment 1 of 50 μ l, the herbal mixture extract of preparation among the embodiment 3 of the Cortex Linderae Obtusilobae extract of preparation and 50 μ l among the embodiment 2 of 50 μ l, making cumulative volume is 200 μ l.With hydroponics in 37 ℃, 5%CO
2Reach 24 hours in the incubator.At this moment, extract should add afterwards, otherwise cell can suffer damage.So, should at first add culture medium and subsequently extract is put in wherein.After cultivation, get 96 orifice plates and remove culture medium.The MTT dyestuff (culture medium of the stock solution of 50 μ l+950 μ l) that adds 50 μ l/ml is with dilute solution, and it distributes 50 μ l in every hole, subsequently at CO
2Further cultivated 4 hours in the incubator.After removing supernatant, add the DMSO of 100 μ l, stirred subsequently 10 minutes.Go up measurement OD with the ELISA reader in 540nm.
In contrast, only add culture medium, and the research viability.
2) NR (dimethyl diaminophenazine chloride: 3-amino-7-dimethylamino-2-toluphenazine) measure
After removing culture medium, add trypsin-EDTA solution of 1ml, it was placed 10～20 minutes.Isolated cell and transferring to subsequently in the falcon pipe of 15ml from container.Join the culture medium of 1ml in the culture bottle and shake, it is also placed the falcon pipe to separate all remaining cells.With the cell suspension of 10 μ l place hematimeter and subsequently pair cell count (2.5 * 10
4Cell/ml).With 50 μ l (1～2 * 10
5Cell)/hole seeds cells on 96 orifice plates, adds 150 μ l culture medium (DMEM+10%FBS+ antibiotic) to it.With cell culture in 37 ℃, 5%CO
2In the incubator 24 hours, obtain cell attachment.
After 24 hours, culture medium is removed the cell on being attached to 96 orifice plates carefully (need careful note making culture medium not to be infected with and to pollute) by cell.The culture medium of 150 μ l is added the Semen Hoveniae extract for preparing with among the embodiment 1 of 50 μ l, the herbal mixture extract of preparation among the embodiment 3 of the Cortex Linderae Obtusilobae extract of preparation and 50 μ l among the embodiment 2 of 50 μ l, making cumulative volume is 200 μ l.With hydroponics in 37 ℃, 5%CO
2In the incubator 24 hours.At this moment, extract should add otherwise cell can suffer damage afterwards.So, should at first add culture medium and subsequently extract is placed in one.
After cultivation, get 96 well culture plates and remove culture medium.The NR dyestuff (culture medium of the stock solution of 10 μ l+990 μ l) that adds 10 μ l/ml is with dilute solution, and it distributes 200 μ l in every hole, subsequently at 37 ℃, and 5%CO
2Further cultivated 3 hours in the incubator.After removing culture medium, with the 1%CaCl of 100 μ l
2With 0.5% formaldehyde wash plate.Remove supernatant.Subsequently, add 1% acetic acid and 50% ethanol of 200 μ l, stirred subsequently 10 minutes.Go up measurement OD with the ELISA reader in 540nm.
In contrast, only add culture medium, and the research viability.
The results are shown in the table 5.
[table 5]
| (Semen Hoveniae+Cortex Linderae Obtusilobae) | ||||
| The Semen Hoveniae extract | 44.60±6.6 | 42.30±4.1 | 98.40±4.1 | 99.00±12 |
| The Cortex Linderae Obtusilobae extract | 95.7±10.9 | 96.7±5.7 | 98.7±3.8 | 97.3±3.5 |
As shown in table 5, Cortex Linderae Obtusilobae extract of the present invention and herbal mixture extract show high cell survival in hepatic cell line.Especially, herbal mixture extract is more effective for improve cell survival in hepatic cell line than Semen Hoveniae extract.Simultaneously, Semen Hoveniae extract of the present invention, Cortex Linderae Obtusilobae extract and herbal mixture extract thereof do not damage the cell survival in the kidney cell line.
Therefore, proved conclusively Semen Hoveniae extract of the present invention, Cortex Linderae Obtusilobae extract and herbal mixture extract thereof have fabulous liver and kidney protective effect.
Hereinafter introduce preparation of compositions embodiment of the present invention.
Be prepared as follows and comprise Semen Hoveniae extract of the present invention, the pharmaceutical composition of Cortex Linderae Obtusilobae extract or its herbal mixture extract.
＜preparation embodiment 1〉preparation of drug combination
1. the preparation of powder
Semen Hoveniae extract (or Cortex Linderae Obtusilobae extract or its herbal mixture extract) 2g
Lactose 1g
Mentioned component is mixed, and airtight bag is filled with described mixture with the preparation powder.
2. the preparation of tablet
Semen Hoveniae extract (or Cortex Linderae Obtusilobae extract or its herbal mixture extract) 100mg
Corn starch 100mg
Lactose 100mg
Magnesium stearate 2mg
Mentioned component is mixed, prepare tablet by tabletting according to the conventional tablet production method.
3. capsular preparation
Semen Hoveniae extract (or Cortex Linderae Obtusilobae extract or its herbal mixture extract) 100mg
Corn starch 100mg
Lactose 100mg
Magnesium stearate 2mg
Mentioned component is mixed, capsule is filled with described mixture with the preparation capsule according to conventional capsule manufacture method.
＜preparation embodiment 2〉preparation of food
Be prepared as follows and comprise Semen Hoveniae extract of the present invention, the food of Cortex Linderae Obtusilobae extract or its herbal mixture extract.
1. the preparation of spice and flavoring agent
Spice and flavoring agent that preparation contains the Semen Hoveniae extract of the present invention (or Cortex Linderae Obtusilobae extract or its herbal mixture extract) of 20～95 weight % are used for improving healthy.
2. the preparation of tomato sauce and raw material (source)
Prepare tomato sauce and raw material in tomato sauce and the raw material and be used for improving healthy by Semen Hoveniae extract of the present invention (or Cortex Linderae Obtusilobae extract or its herbal mixture extract) being joined with 0.2～1.0 weight %.
3. the preparation of wheaten food
With 0.5～5.0 weight % Semen Hoveniae extract of the present invention (or Cortex Linderae Obtusilobae extract or its herbal mixture extract) is joined in the flour, and described mixture is used to prepare bread, cake, cookies, crispbread and noodles improve healthy food to produce.
4. the preparation of soup and gravy
Improve healthy finished meat, noodle soup and gravy by Semen Hoveniae extract of the present invention (or Cortex Linderae Obtusilobae extract or its herbal mixture extract) being added to prepare in soup and the gravy with 0.1～5.0 weight %.
5. grind the preparation of beef
Improve healthy grinding beef by Semen Hoveniae extract of the present invention (or Cortex Linderae Obtusilobae extract or its herbal mixture extract) being joined to grind to prepare in the beef with 10 weight %.
6. the preparation of milk product
With 5～10 weight % Semen Hoveniae extract of the present invention (or Cortex Linderae Obtusilobae extract or its herbal mixture extract) is added the Ruzhong, use it to produce subsequently and improve healthy milk product, comprise butter and ice cream.
7.cerial preparation
By conventional method with brown rice, Fructus Hordei Vulgaris, Oryza glutinosa and Semen Coicis (Job ' s-tears) agglutination, dry and baking is pulverized with pulverizer subsequently, obtains the granule of 60-mesh.
Steam Semen sojae atricolor by conventional method, Semen Sesami Nigrum, Perilla japonica, dry and baking is pulverized with pulverizer subsequently, causes into the granule of 60-mesh.
Semen Hoveniae extract of the present invention (or Cortex Linderae Obtusilobae extract or its herbal mixture extract) is concentrated under decompression in the vacuum concentration instrument, carry out drying by spray dryer subsequently.Exsiccant product is ground into the granule of 60-mesh.
Mix crop, the dry powder of seed and Semen Hoveniae extract (or Cortex Linderae Obtusilobae extract or its herbal mixture extract) by following ratio.
Crop (brown rice 30 weight %, Semen Coicis 15 weight %, Fructus Hordei Vulgaris 20 weight %),
Seed (Perilla japonica 7 weight %, Semen sojae atricolor 8 weight %, Semen Sesami Nigrum 7 weight %),
The dry powder (3 weight %) of Semen Hoveniae extract (or Cortex Linderae Obtusilobae extract or its herbal mixture extract),
Ganoderma (Ganoderma lucidum) (0.5 weight %),
Radix Rehmanniae (Rehmannia glutinosa) (0.5 weight %)
＜preparation embodiment 3〉preparation of beverage
1. the preparation of soda pop
With sugar (5～10%), citric acid (0.05～0.3%), caramel (0.005～0.02%) and vitamin C (0.1～1%) mix, and to its water (79～94%) that adds purification, obtain syrup.With described syrup in 85～98 ℃ of sterilizations 20～180 seconds, subsequently with 1: 4 ratio and cold water mix.With 0.5～0.82% to injecting carbon dioxide wherein, cause comprising the preparation of the soda pop of Semen Hoveniae extract of the present invention (or Cortex Linderae Obtusilobae extract or its herbal mixture extract).
2. the preparation of healthy beverage
With optional component such as liquid fructose (0.5%), oligosaccharide (2%), sugar (2%), salt (0.5%), water (75%) and Semen Hoveniae extract (or Cortex Linderae Obtusilobae extract or its herbal mixture extract) uniform mixing.After the pulverizing, mixture is placed small container such as pet or vial, obtain the preparation of healthy beverage.
3. the preparation of plant juice
5g Semen Hoveniae extract of the present invention (or Cortex Linderae Obtusilobae extract or its herbal mixture extract) is added to 1, improves healthy plant juice with preparation in 000ml Fructus Lycopersici esculenti or the Radix Dauci Sativae juice.
4. the preparation of fruit juice
1g Semen Hoveniae extract of the present invention (or Cortex Linderae Obtusilobae extract or its herbal mixture extract) is added to 1, improves healthy fruit juice with preparation in the Fructus Mali pumilae of 000ml or the Sucus Vitis viniferae.
[industrial applicability]
Northern trifoliate orange Dulcis extract of the present invention, Cortex Linderae Obtusilobae extract or its herbal mixture extract are effective for improving the liver function height, because it can reduce the GOT as main liver function index, GPRT, ALP, the level of BUN and total bilirubin. ALP and BUN also are used as renal function index, so the reduction of the ALP that extract of the present invention causes and BUN level illustrates northern trifoliate orange Dulcis extract of the present invention, Cortex Linderae Obtusilobae extract or its herbal mixture extract can also improve renal function.
Northern trifoliate orange Dulcis extract of the present invention, Cortex Linderae Obtusilobae extract or its herbal mixture extract can also reduce the amount of hydroxy-proline in the liver but improve the amount of hydroxy-proline in the kidney, and prompting said extracted thing has splendid fibrosis and kidney protective effect.
In addition, northern trifoliate orange Dulcis extract of the present invention, Cortex Linderae Obtusilobae extract or its herbal mixture extract can reduce the level of the index MDA of lipid peroxidation in liver and the nephridial tissue, point out described extract to have splendid antioxidation.
Northern trifoliate orange Dulcis extract of the present invention, Cortex Linderae Obtusilobae extract or its herbal mixture extract promote the cell survival in liver and the kidney cell line, illustrate that described extract has splendid liver and nephrocyte protective effect.
Therefore, composition of the present invention not only can be used for anti-oxidant and fibrosis effectively, can also for the protection of with improve liver and renal function.
Claims (8)
1. antioxidative compositions, it comprises that Cortex Linderae Obtusilobae (Lindera obtusiloba) water extract is as effective ingredient.
2. antioxidative compositions, it water extract of herbal mixture that comprises Semen Hoveniae (Hovenia dulcis Thunb) and Cortex Linderae Obtusilobae is as effective ingredient, and wherein the dry weight ratio of Semen Hoveniae and Cortex Linderae Obtusilobae is 2: 1～1: 1 in described herbal mixture.
3. the compositions of a fibrosis, it water extract that comprises Cortex Linderae Obtusilobae is as effective ingredient.
4. the compositions of a fibrosis, it water extract of herbal mixture that comprises Semen Hoveniae and Cortex Linderae Obtusilobae is as effective ingredient, and wherein the dry weight ratio of Semen Hoveniae and Cortex Linderae Obtusilobae is 2: 1～1: 1 in described herbal mixture.
5. compositions of improving liver function, it water extract that comprises Cortex Linderae Obtusilobae is as effective ingredient.
6. compositions of improving liver function, it water extract of herbal mixture that comprises Semen Hoveniae and Cortex Linderae Obtusilobae is as effective ingredient, and wherein the dry weight ratio of Semen Hoveniae and Cortex Linderae Obtusilobae is 2: 1～1: 1 in described herbal mixture.
7. compositions of improving renal function, it water extract that comprises Cortex Linderae Obtusilobae is as effective ingredient.
8. compositions of improving renal function, it water extract of herbal mixture that comprises Semen Hoveniae and Cortex Linderae Obtusilobae is as effective ingredient, and wherein the dry weight ratio of Semen Hoveniae and Cortex Linderae Obtusilobae is 2: 1～1: 1 in described herbal mixture.
Applications Claiming Priority (10)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020040006437A KR100586269B1 (en) | 2004-01-31 | 2004-01-31 | Composition comprising Lindera obtusiloba extract |
| KR1020040006436 | 2004-01-31 | ||
| KR10-2004-0006435 | 2004-01-31 | ||
| KR1020040006437 | 2004-01-31 | ||
| KR10-2004-0006437 | 2004-01-31 | ||
| KR10-2004-0006436A KR100536855B1 (en) | 2004-01-31 | 2004-01-31 | Composition comprising Hovenia dulcis Thunb. extract for improvement of kidney function |
| KR10-2004-0006435A KR100536856B1 (en) | 2004-01-31 | 2004-01-31 | Composition comprising herbal mixture extract |
| KR1020040006435 | 2004-01-31 | ||
| KR10-2004-0006436 | 2004-01-31 | ||
| PCT/KR2005/000283 WO2005072758A1 (en) | 2004-01-31 | 2005-01-31 | Composition comprising hovenia dulcis thunb. extract, lindera obtusiloba blume extract, or herbal mixture extract thereof |
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| CN1913910B true CN1913910B (en) | 2011-02-16 |
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| KR101124442B1 (en) * | 2009-09-14 | 2012-03-21 | 영남대학교 산학협력단 | Composition comprising the extract of Lindera obtusiloba for preventing and improving inflammatory or allergic diseases |
| KR101039628B1 (en) * | 2009-11-20 | 2011-06-08 | 양지화학 주식회사 | Composition Comprising the Extracts of Lindera obtusiloba as Effective Ingredient for Prevention and Treatment of Thrombosis |
| CN103830540A (en) * | 2012-11-20 | 2014-06-04 | 浙江红石梁集团天台山乌药有限公司 | Preparation technology for compound Lindera aggregata jujube kernel extract |
| CN112088996A (en) * | 2020-10-13 | 2020-12-18 | 湖南尚道生物科技有限公司 | Lactobacillus fermented beverage for protecting liver and alleviating hangover and preparation method thereof |
| KR20220062834A (en) | 2020-11-09 | 2022-05-17 | 지성일 | Method for manufacturing reagent for liver function diagnosis |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1448166A (en) * | 2002-04-03 | 2003-10-15 | 顾奎琴 | Pure natural infusion for relieving alcoholism and protecting liver and prep. thereof |
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|---|---|---|---|---|
| CN1448166A (en) * | 2002-04-03 | 2003-10-15 | 顾奎琴 | Pure natural infusion for relieving alcoholism and protecting liver and prep. thereof |
Non-Patent Citations (6)
| Title |
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| CHO J.Y. ET AL:.＇Isolation and identification of3-methoxy-4-hydroxybenzoic acid and3-methoxy-4-hydroxycinnamic acid from hot water extracts ofHovenia dulcis THUNB, and confirmation of theirantioxidative and antimicrobial activity＇.KOR. J. FOOD SCI.32 6.2000,32(6),1403-1408. |
| CHO J.Y. ET AL:.＇Isolation and identification of3-methoxy-4-hydroxybenzoic acid and3-methoxy-4-hydroxycinnamic acid from hot water extracts ofHovenia dulcis THUNB, and confirmation of theirantioxidative and antimicrobial activity＇.KOR. J. FOOD SCI.32 6.2000,32(6),1403-1408. * |
| HASE K. ET AL.＇Hepatoprotective effect of Hovenia dulcisTHUNB, on experimental liver injuries induced by carbontetrachloride or D-galactosamine/lipopolysaccharide＇.BIOL. PHARM. BULL.20 4.1997,20(4),381-385. |
| HASE K. ET AL.＇Hepatoprotective effect of Hovenia dulcisTHUNB, on experimental liver injuries induced by carbontetrachloride or D-galactosamine/lipopolysaccharide＇.BIOL. PHARM. BULL.20 4.1997,20(4),381-385. * |
| YOSHIKAWA M. ET AL:.＇Bioactive constituents of Chinesenatural medicine.III. Absolute stereostructures of newdihydroflavonols, hovenitins I,II,III, isolated fromhoveniae semen seufrctus, the seed and fruit of Hoveniadulcis THUNB.(Rhamnaceae): Inhibitory effect on alcohol...＇.YAKUGAKU ZASSHI117 2.1997,117(2),108-118. |
| YOSHIKAWA M. ET AL:.＇Bioactive constituents of Chinesenatural medicine.III. Absolute stereostructures of newdihydroflavonols, hovenitins I,II,III, isolated fromhoveniae semen seufrctus, the seed and fruit of Hoveniadulcis THUNB.(Rhamnaceae): Inhibitory effect on alcohol...＇.YAKUGAKU ZASSHI117 2.1997,117(2),108-118. * |
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| KR20050078693A (en) | 2005-08-08 |
| CN1913910A (en) | 2007-02-14 |
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