CN1861637A - Preparation process of Huai Chinese yam polysaccharose and use thereof - Google Patents
Preparation process of Huai Chinese yam polysaccharose and use thereof Download PDFInfo
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- CN1861637A CN1861637A CN 200610085512 CN200610085512A CN1861637A CN 1861637 A CN1861637 A CN 1861637A CN 200610085512 CN200610085512 CN 200610085512 CN 200610085512 A CN200610085512 A CN 200610085512A CN 1861637 A CN1861637 A CN 1861637A
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Abstract
A process for extracting polyose from yam includes such steps as peeling, slicing, drying in the air, extracting in boiling water, filtering, centrifugal separation to obtain supernatant, adding alcohol, educing out, centrifugal separation of deposit to obtain crude polyose, dissolving in water, educing out, filtering and washing with absolute alcohol. It can be used for health-care food to decrease blood sugar, improve immunity, and delay senility.
Description
Technical field
The present invention relates to preparation process of Huai Chinese yam polysaccharose and uses thereof.
Background technology
The record the earliest of Chinese yam sees in the Classic of Mountains and Rivers, and Shennong's Herbal row Chinese yam is top grade.Except that the name of Chinese yam, also have all administrations, administration Henan, potato medicine in the book on Chinese herbal medicine, affix one's name to titles such as pre-.In Su Song " figure is through book on Chinese herbal medicine ", TANG Shen-Wei " Classified Materia Medica ", LI Shi-Zhen Compendium of Material Medica, Wu Qijun books such as " An Illustrated Book on Plants ", record is arranged all.Promptly be utilized as top grade qi-restoratives protective foods since ancient times by compatriots, tradition name side is as all having Chinese yam in pill of Six Ingredients, eight flavor ball, shenling baizhu powder and the four refreshing soup, successive dynasties utilize Chinese yam assembly prescription reach 300 surplus the side, occupying crucial status in Chinese medicine, is the very high integration of drinking and medicinal herbs food of nutritive value.As the Chinese yam face in the principle of Correct Diet, but the informal dress qi-restoratives is thin, beneficial vigour.Splendid qi-restoratives health product are prolonged life, are in body-building.
Rhizoma dioscoreae derives from the stem tuber of Dioscoreaceae Wild yam Dioscorea L Chinese yam group Sect.Enantiophyllum plant Chinese yam Dioscoreaopposita Thunb., ginseng potato D.alata L., Dioscarea persimilis Prain et Burkill D.persimilis Prain et Burkill or mountain potato D.fordii Prain etBurkill, the equal edible of stem tuber of most kind plants in the Chinese yam group, and have similar nutrition and pharmaceutical use.Chinese yam is distributed widely in various places, and both edible was pharmaceutically acceptable again." different garden " cloud of uncle Liu Jing of the Northern and Southern Dynasties: " sweet potato of Chinese yam, root not only can be used as medicine but also multiple edible." the existing best place of production of book on Chinese herbal medicine record in ancient times, think that as Collective Notes to the Canon of Materia medica (modern Jiangxi) is maximum and beautiful between Nankang; Tang Materia Medica thinks that Shu Dao (modern Sichuan) person is good." figure is through book on Chinese herbal medicine " thinks north all (modern Taiyuan, Shanxi), four bright (modern Zhejiang Siming Shan) person's the best.The self-evident branch that gradually occurs medicinal Chinese yam and edible Chinese yam backwards, according to herbal for Relief of Famines and " An Illustrated Book on Plants " record, medicinal Chinese yam distributes northern.Someone thinks that the Chinese yam drug effect that originates in Huaihe River one band is best, the Chinese yam that just specially refers to originate in Huaihe River with " Rhizoma dioscoreae ".
Chinese yam is all previous " kind that Chinese pharmacopoeia is recorded, the Chinese yam sweet property of distinguishing the flavor of is flat, the gas of tonifying the spleen and stomach, can mend the moon of stomach again, be used for the treatment of weakness of the spleen and stomach, as food less, burnout, loose stool, rush down for a long time, infantile malnutrition, women's lukorrhagia due to hypofunction of the spleen etc., normal and the use of genseng (or Radix Codonopsis) bighead atractylodes rhizome compatibility are as name side's shenling baizhu powder.Chinese yam energy help lung, kidney is used for the YIN-deficiency of the lung and kidney disease.As chronic cough of deficiency lung, or the deficiency of both the lung and kidney chronic cough breathes heavily for a long time, often joins the tuber of dwarf lilyturf, shizandra berry is equal to usefulness.Chinese yam treatment emission due to deficiency of the kidney, diseases such as frequent micturition, normal and Radix Rehmanniae Preparata, Fructus Corni, South Dodder Seed Chinese Dodder Seed are equal to usefulness.Chinese yam treatment diabetes (diabetes) can independent heavy dose of decocting for tea-drinking, also can join the wind-weed, Snakegourd Root, glutinous rehmannia are equal to usefulness, bright Chinese yam is mashed the external application tuberculosis, swollen from the beginning of carbuncle that can dissipate.The bighead atractylodes rhizome and Chinese yam can the tonifying spleen invigorating the spleen, but the bighead atractylodes rhizome is dry, Chinese yam property profit.Dampness blocking in middle-JIAO, the bighead atractylodes rhizome is suitable; Deficiency of YIN of both the lung and kidney, then Chinese yam is the most suitable.Rhizoma dioscoreae output is huge, all is basically at present to use as raw medicinal material or food, does not have deep processed product, and added value is low.The present invention's Rhizoma dioscoreae is a raw material, through preparation for processing carry polysaccharide, further improved Rhizoma dioscoreae quality product and added value.
Summary of the invention
The used Rhizoma dioscoreae of the present invention means the tame Rhizoma dioscoreae Dioscorea of Basin of Huaihe River opposita L..
The purpose of this invention is to provide a kind of preparation process of Huai Chinese yam polysaccharose.
Purpose of the present invention can reach by following measure:
A kind of method of extracting Huai Chinese yam polysaccharose is characterized by: adopt the hot water lixiviate, the extraction process condition is: extraction temperature 70-90 ℃, and extraction time 4-10h, the weight ratio of dried raw material of Rhizoma dioscoreae and amount of water is 1: 30-1: 100.
Another object of the present invention provides a kind of Huai Chinese yam polysaccharose in hypoglycemic, the Rezulin of treatment and the application in enhance immunity power, anti-ageing and antioxidant.
The feature of preparation process of Huai Chinese yam polysaccharose of the present invention is the earth that fresh Rhizoma dioscoreae stem tuber is scraped off gently the surface, the epidermis of pruning, and crosscut places rapidly under the daylight and dries into about 2～3mm thin slice, in case brownization of Rhizoma dioscoreae; The air dried Rhizoma dioscoreae is pulverized, and the dress sealing bag places drying basin standby.Add the 30-100 distillation water yield doubly by every gram dry weight, in 70-90 ℃ of boiling water bath lixiviate 4-10h.Extracting liquid filtering in the centrifugal 5min of 3000rmin-1, is got filtrate supernatant liquor adding volume ethanol and is left standstill, and alcohol is analysed.It is centrifugal to separate out precipitation, gets polysaccharide crude, and water redissolves, alcohol is analysed, and filters, and the absolute alcohol washing obtains total polysaccharides.Carry out the hypoglycemic experiment through ICR kind mouse animal, the experiment of the effect of ICR kind mouse lymphocyte multiplication capacity, strengthen mouse carbon and clean up the experiment of the effect of ability, cause that the experiment of SOD vigor and MDA content shows in aging model mice serum and the brain, that the total polysaccharides that the present invention obtains can be used as is hypoglycemic, improve humoral immunity of organism and cellular immunity, anti-oxidant, antidotal heath food.Rhizoma dioscoreae output is huge, because the correlative study basis is not enough, all is to use as medicinal material or food basically, does not have deep processed product, and added value is low.
The invention has the beneficial effects as follows:
The plant material wide material sources that the present invention utilizes, holding concurrently again is the medicine-food two-purpose kind, security is good, the production technique simple and effective, health care and adjuvant treatment effect are definite.It is the effective way that further improves Rhizoma dioscoreae product and added value.
Embodiment
Embodiment 1
Fresh Rhizoma dioscoreae stem tuber is also scraped off surperficial earth gently, the epidermis of pruning, crosscut places rapidly under the daylight and dries into about the 2-3mm thin slice, in case brownization of Rhizoma dioscoreae; Air dried Rhizoma dioscoreae 100 grams are pulverized, and the dress sealing bag places drying basin standby.Add the 3000 distillation water yields that restrain by every gram dry weight, in 70 ℃ of boiling water bath lixiviate 4h.Extracting liquid filtering, with filtrate in 3000rmin
-1Centrifugal 5min gets supernatant liquor adding volume ethanol and leaves standstill, and alcohol is analysed.It is centrifugal to separate out precipitation, gets polysaccharide crude, and water redissolves, alcohol is analysed, and filters, and the absolute alcohol washing obtains total polysaccharides.
ICR kind mouse 18-22g, male.Sub-cage rearing is indoor in each 12 hours the experimentation on animals of 20 ± 2 ℃ of light and shades, and laboratory animal freely drinks water and takes food.The normal nursing 2 days behind the fasting 20hr, pressed 90mg/kg IV injection tetraoxypyrimidine solution, and injection finishes in the 30sce, measures fasting plasma glucose behind the 72hr.Select for use blood glucose value to carry out the hypoglycemic experiment greater than the animal of 11.1mmol/ml.
Animal is divided into 6 groups at random by blood glucose value, 10 every group: dosage group (400mg/kg), Rhizoma dioscoreae Crude polysaccharides high dose group (800mg/kg) in normal group, model control group, positive group (N1,N1-Dimethylbiguanide 100mg/kg), Rhizoma dioscoreae Crude polysaccharides low dose group (200mg/kg), the Rhizoma dioscoreae Crude polysaccharides.Each administration group is pressed 10ml/kg volume gastric infusion (IG), and normal group and model group give equivalent distilled water.After IG14 days, fasting 5hr gets blood through eye socket after last administration continuously, measures blood sugar.The result is as follows:
| Groups | Dose(mg/kg) | Glu(mmol/l) | Velocity of variation (%) |
| Model control group | - | 25.91±4.19 * | |
| The N1,N1-Dimethylbiguanide group | 100 | 20.07±5.03 | -22.53 |
| The Chinese yam polysaccharide group | 200 | 22.21±7.05 | -14.29 |
| The Chinese yam polysaccharide group | 400 | 20.94±6.03 * | -19.17 |
| The Chinese yam polysaccharide group | 800 | 20.19±4.18 ** | -22.08 |
*P＜0.01
*P＜0.05 vs model control group
Dosage is compared blood sugar with high dose group and has been descended 19.17% and 22.08% respectively in the Huai Chinese yam polysaccharose with model group, have significant difference (p＜0.05 or this＜0.01), show that the Rhizoma dioscoreae Crude polysaccharides has hypoglycemic activity to this tetraoxypyrimidine type diabetic mice.
Embodiment 2
Fresh Rhizoma dioscoreae stem tuber is also scraped off surperficial earth gently, the epidermis of pruning, crosscut places rapidly under the daylight and dries into about the 2-3mm thin slice, in case brownization of Rhizoma dioscoreae; Air dried Rhizoma dioscoreae 200 grams are pulverized, and the dress sealing bag places drying basin standby.Add the 20000 distillation water yields that restrain by every gram dry weight, in 90 ℃ of boiling water bath lixiviate 10h.Extracting liquid filtering, with filtrate in 3000rmin
-1Centrifugal 5min gets supernatant liquor adding volume ethanol and leaves standstill, and alcohol is analysed.It is centrifugal to separate out precipitation, gets polysaccharide crude.
The Rhizoma dioscoreae Crude polysaccharides is mixed with suspension with distilled water.
ICR kind mouse 18-22g, male; Cavy.Edible full-valence pellet feed, the drinking-water of freely ingesting.
The YAC-1 cell.
Healthy sheep jugular vein is got blood, and the sterilization Erlenmeyer flask of sheep blood being put into granulated glass sphere shakes towards a direction, with defiber, is stored in 4 ℃ of refrigerators with A Shi liquid and preserves standby.The centrifugal 10min washing of time spent physiological saline 2000r/min three times.Gather guinea pig blood, isolate serum (pooled serums of at least 5 cavys), preceding 1mL hematocrit SRBC is joined in the 5mL guinea pig serum, put 4 ℃ of refrigerator 30min, often vibration, centrifuging and taking supernatant, packing bottle ,-70 ℃ of preservations.Time spent dilutes by 1: 10 with physiological saline.
Mouse is divided into control group (giving distilled water) and Chinese yam polysaccharide 200mg/kg., 400mg/kg and 3 dosage groups of 800mg/kg, 10 every group at random.Irritate the long-pending 0.1ml/10g bw of body of stomach, 1 time/d, continuous 8d.
RPMI-1640 filtering with microporous membrane degerming, add 10% calf serum, 1% glutamine (200/ml), penicillin (100/ml), Streptomycin sulphate (100L/ml) and 2 mercapto ethanol (5 μ mol/ml), transfer PH to 7.0～7.2 with aseptic HCl (1mol/L) or aseptic NaOH (1mol/L), make complete culture solution; PBS damping fluid with 7.2～7.4 is made solvent, matching while using MTT solution (5mg/ml); Aseptic NaHCO with 3.5%
3Aseptic Hanks liquid PH is transferred to 7.2～7.4; Be mixed with the ConA liquid of 100 μ g/ml with distilled water, after the filtration sterilization ,-20 ℃ of preservations; In the 96ml Virahol, add 4ml HCl (1mol/L) and make the acid isopropyl alcoholic solution.Each treated animal continuous irrigation stomach to the 8d, the aseptic spleen of getting is put to death in every mouse cervical vertebra dislocation, in the little plate that fills an amount of aseptic Hanks liquid, gently spleen is torn up with tweezers, make the individual cells suspension, 200 orders filter, and repeatedly wash, be suspended in the 2ml complete culture solution after centrifugal with Hanks liquid, with the blue dyeing of the platform phenol viable cell that counts, adjusting cell concn is 2 * 106/ml, divide two holes to put into culture hole, every hole 1ml, a hole adds 50 μ l Con A liquid, 5%CO is put in contrast in another hole
2, cultivate 72hr for 37 ℃, to cultivate and finish preceding 4hr, supernatant liquor 0.7ml is inhaled in every hole gently, adds the RPMI-1640 that 0.7ml does not contain calf serum, adds 50 μ l MTT liquid simultaneously, continues to cultivate 4h.Cultivate and finish, every hole adds 1ml acid isopropyl alcohol, and the piping and druming mixing dissolves purple crystal fully, moves in the 1ml cuvette, and in spectrophotometer, the 570nm place measures optical density(OD).
| Group | Dosage (mg/kg) | Multiplication capacity |
| The normal control group | - | 0.014±0.0059 |
| Chinese yam polysaccharide | 200 | 0.014±0.012 |
| Chinese yam polysaccharide | 400 | 0.063±0.049 * |
| Chinese yam polysaccharide | 800 | 0.077±0.041 ** |
*P＜0.05
*Compare with the normal control group p＜0.01
When above result shows Rhizoma dioscoreae Crude polysaccharides 400,800mg/kgbg/d dosage, has the effect that strengthens the mouse lymphocyte multiplication capacity.
Embodiment 3
Fresh Rhizoma dioscoreae stem tuber is also scraped off surperficial earth gently, the epidermis of pruning, crosscut places rapidly under the daylight and dries into about the 2-3mm thin slice, in case brownization of Rhizoma dioscoreae; Air dried Rhizoma dioscoreae 150 grams are pulverized, and the dress sealing bag places drying basin standby.Add the 7500 distillation water yields that restrain by every gram dry weight, in 80 ℃ of boiling water bath lixiviate 6h.Extracting liquid filtering, with filtrate in 3000rmin
-1Centrifugal 5min gets supernatant liquor adding volume ethanol and leaves standstill, and alcohol is analysed.It is centrifugal to separate out precipitation, gets polysaccharide crude.
The Rhizoma dioscoreae Crude polysaccharides is mixed with suspension with distilled water.
Each treated animal continuous irrigation stomach is during to 3d, every mouse abdominal injection 2% (v/v) sheep red blood cell (SRBC) suspension 0.2mL.Behind the 5d (during do not stop to irritate stomach), mouse is got blood in centrifuge tube through eye socket, places 1h, and the centrifugal 15min of 3500r/min collects serum, and serum is with 320 times of normal saline buffer solution dilutions.Serum 1mL after the dilution is put in vitro, add 10% (v/v) SRBC0.5mL successively, complement 1mL (diluting by 1: 10) with physiological saline, other establishes the not control tube of increase serum (with physiologic saline for substitute).After putting in 37 ℃ of waters bath with thermostatic control insulation 30min, the ice bath termination reaction.The centrifugal 10min of 2000r/min.Get supernatant liquor 1mL, Dou Shi reagent 3mL gets 10% (v/v) SRBC 0.25mL simultaneously and adds Dou Shi reagent to 4mL in vitro, in another in vitro, and as the half hemolysis value of SRBC.Fully mixing behind the placement 10min, is sentenced control tube in 540nm and is made blank, measures respectively and respectively manages optical density value.The amount of hemolysin is with half hemolysis value (HC
50) expression, calculate by following formula:
Half hemolysis value (the HC of each mouse
50Optical density(OD) * extension rate result during)=sample hose optical density(OD)/SRBC HD50 is as follows:
| Group | Dosage (mg/kg) | Multiplication capacity |
| The normal control group | - | 0.014±0.0059 |
| Chinese yam polysaccharide | 200 | 0.014±0.012 |
| Chinese yam polysaccharide | 400 | 0.063±0.049 * |
| Chinese yam polysaccharide | 800 | 0.077±0.041 ** |
*Compare with the normal control group P＜0.05
The Rhizoma dioscoreae total polysaccharides has the enhancing antibody nucleus formation.
Embodiment 4
Fresh Rhizoma dioscoreae stem tuber is scraped off surperficial earth gently, the epidermis of pruning, crosscut places rapidly under the daylight and dries into about the 2-3mm thin slice, in case brownization of Rhizoma dioscoreae; Air dried Rhizoma dioscoreae 100 grams are pulverized, and the dress sealing bag places drying basin standby.By every gram dry weight add 7000 can the distillation water yield, in 75 ℃ of boiling water bath lixiviate 8h.Extracting liquid filtering, with filtrate in 3000rmin
-1Centrifugal 5min gets supernatant liquor adding volume ethanol and leaves standstill, and alcohol is analysed.It is centrifugal to separate out precipitation, gets polysaccharide crude.
The Rhizoma dioscoreae Crude polysaccharides is mixed with suspension with distilled water.
India ink is made injection prepared Chinese ink for 4 times with the physiological saline dilution, take by weighing 1.0g Na
2CO
3Adding distil water is configured to 0.1%Na to 1000mL
2CO
3Solution.Each treated animal continuous irrigation stomach is during to 8d, and every caudal vein injects injection prepared Chinese ink (0.1mL/10gbw), and timing immediately after the injection is got blood 20 μ L from the angular vein clump respectively in injecting back 0.5min, 6min, is added to 2mL Na
2CO
3In the solution, with the spectrophotometer meter at 600nm wavelength place's photometry density value, with Na
2CO
3Solution is as blank.Mouse is put to death in the cervical vertebra dislocation, gets liver, spleen, blots the organ surface blood stains with filter paper, weighs.Clean up index K by following formula calculating:
Clean up index K=(logOD0.5-logOD6)/(t6-t0.5)
The result is as follows:
| Group | Dosage (mg/kg) | Clean up index |
| The normal control group | - | 0.076±0.016 |
| Chinese yam polysaccharide | 200 | 0.082±0.012 |
| Chinese yam polysaccharide | 400 | 0.091±0.022 |
| Chinese yam polysaccharide | 800 | 0.098±0.011 ** |
*Compare with the normal control group P＜0.01: the Rhizoma dioscoreae Crude polysaccharides has the effect that enhancing mouse carbon is cleaned up ability.
Example 4 proofs gained Rhizoma dioscoreae total polysaccharides of the present invention can be used for enhance immunity power.
Embodiment 5
Fresh Rhizoma dioscoreae stem tuber is also scraped off surperficial earth gently, the epidermis of pruning, crosscut places rapidly under the daylight and dries into about the 2-3mm thin slice, in case brownization of Rhizoma dioscoreae; Air dried Rhizoma dioscoreae 200 grams are pulverized, and the dress sealing bag places drying basin standby.Add the 16000 distillation water yields that restrain by every gram dry weight, in 85 ℃ of boiling water bath lixiviate 9h.Extracting liquid filtering, with filtrate in 3000rmin
-1Centrifugal 5min gets supernatant liquor adding volume ethanol and leaves standstill, and alcohol is analysed.It is centrifugal to separate out precipitation, gets polysaccharide crude.
The Rhizoma dioscoreae Crude polysaccharides is mixed with suspension with distilled water.
ICR kind mouse 18-22g, male.
The ICR mouse 10 every group, is divided into into following 5 groups according to the body weight random packet: normal control group, model group, positive controls (gynostemma pentaphylla total saponins 100mg/kg), Chinese yam polysaccharide low dose group (100mg/kg) and high dose group (200mg/kg).The subcutaneous injection physiological saline 0.1ml/10g of normal control group nape every day portion wherein, all the other respectively organize the subcutaneous injection D-of nape portion semi-lactosi 200mg/kg every day.Subcutaneous injection simultaneously each administration group is irritated stomach by 0.1ml/10g give different pharmaceutical every day, and model group, normal group are irritated stomach and give equivalent physiological saline every day.Each mouse is weighed record once weekly in experimentation, observes mode of appearance.Irritate stomach in the time of the 30th day after two hours, mouse orbit is got blood, the centrifugal 15min of 3500r/min, and it is standby to get upper serum, puts to death mouse, gets cerebral tissue.Press kit method and measure MDA, SOD in blood and the cerebral tissue.
MDA in the serum and SOD content
| Group | Dosage mg/kg | MDA(nmol/ml) | SOD(U/ml) |
| The normal control group | - | 3.23±1.69 ** | 64.77±5.23 ** |
| Model control group | - | 5.45±1.51 | 36.15±6.26 |
| Chinese yam polysaccharide | 100 | 4.50±0.99 | 49.73±13.16 ** |
| Chinese yam polysaccharide | 200 | 4.05±1.18 * | 52.60±10.40 ** |
**P＜0.01
*P＜0.05 VS MOD
MDA in the cerebral tissue and SOD content
| Group | Dosage mg/kg | MDA(nmol/g) | SOD(U/g) |
| The normal control group | - | 45.41±23.42 * | 141.33±9.25 ** |
| Model control group | - | 76.24±34.79 | 97.49±24.21 |
| The Rhizoma dioscoreae Crude polysaccharides | 100 | 48.77±13.24 * | 123.88±30.85 * |
| The Rhizoma dioscoreae Crude polysaccharides | 200 | 45.61±15.09 * | 129.76±16.20 ** |
ΔΔP＜0.01 VS Nor，
**P＜0.01
*P＜0.05VS MOD
Huai Chinese yam polysaccharose can significantly increase the D-semi-lactosi and cause SOD vigor in aging model mice serum and the brain, reduces MDA content, has anti-oxidant, anti-aging effects.
Claims (4)
1. method of extracting Huai Chinese yam polysaccharose is characterized by: adopt the hot water lixiviate, the extraction process condition is: extraction temperature 70-90 ℃, and extraction time 4-10h, the weight ratio of dried raw material of Rhizoma dioscoreae and amount of water is 1: 30-1: 100.
2. the Rhizoma dioscoreae total polysaccharides that extracts of method according to claim 1, it consists of the mixed polysaccharide that D-semi-lactosi, D-seminose, glucose, D-pectinose are formed, and molecular weight is 45970Dal～45990Dal.
3. Huai Chinese yam polysaccharose according to claim 1, its preparation is hypoglycemic in treatment, the application in the Rezulin.
4. Huai Chinese yam polysaccharose according to claim 1, the application of its preparation in enhance immunity power, anti-ageing and antioxidant.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103520425A (en) * | 2013-10-18 | 2014-01-22 | 广西中医药大学 | Preparation method and application of effective part of dioscorea persimilis for decreasing blood sugar |
| CN104448017A (en) * | 2014-11-27 | 2015-03-25 | 秦富英 | Method for extracting Chinese yam oligosaccharide |
| CN107137420A (en) * | 2017-07-05 | 2017-09-08 | 无限极（中国）有限公司 | A kind of mulberry leaf-chrysanthemum Chinese yam complex polysaccharide with clearing heat and nourishing yin function of blood sugar reduction and preparation method thereof |
| CN107722134A (en) * | 2017-11-08 | 2018-02-23 | 广西南宁胜祺安科技开发有限公司 | A kind of preparation method of Huai Chinese yam polysaccharose |
| WO2018103723A1 (en) * | 2016-12-10 | 2018-06-14 | 高尚先 | New use for mannose for increasing treg cell number and foxp3 factor expression level thereof |
| CN111763267A (en) * | 2020-06-19 | 2020-10-13 | 山东天智绿业生物科技有限公司 | Method for extracting Chinese yam polysaccharide and protein |
| CN114957500A (en) * | 2021-12-24 | 2022-08-30 | 济宁医学院 | Selenylation modification method for improving immunocompetence of Chinese yam polysaccharide and application thereof |
-
2006
- 2006-06-20 CN CN 200610085512 patent/CN1861637A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103520425A (en) * | 2013-10-18 | 2014-01-22 | 广西中医药大学 | Preparation method and application of effective part of dioscorea persimilis for decreasing blood sugar |
| CN103520425B (en) * | 2013-10-18 | 2016-03-23 | 广西中医药大学 | The preparation method and its usage of Dioscarea persimilis Prain et Burkill． blood-sugar-lowering effective parts |
| CN104448017A (en) * | 2014-11-27 | 2015-03-25 | 秦富英 | Method for extracting Chinese yam oligosaccharide |
| WO2018103723A1 (en) * | 2016-12-10 | 2018-06-14 | 高尚先 | New use for mannose for increasing treg cell number and foxp3 factor expression level thereof |
| CN107137420A (en) * | 2017-07-05 | 2017-09-08 | 无限极（中国）有限公司 | A kind of mulberry leaf-chrysanthemum Chinese yam complex polysaccharide with clearing heat and nourishing yin function of blood sugar reduction and preparation method thereof |
| CN107722134A (en) * | 2017-11-08 | 2018-02-23 | 广西南宁胜祺安科技开发有限公司 | A kind of preparation method of Huai Chinese yam polysaccharose |
| CN111763267A (en) * | 2020-06-19 | 2020-10-13 | 山东天智绿业生物科技有限公司 | Method for extracting Chinese yam polysaccharide and protein |
| CN114957500A (en) * | 2021-12-24 | 2022-08-30 | 济宁医学院 | Selenylation modification method for improving immunocompetence of Chinese yam polysaccharide and application thereof |
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