QISHEN YIQI DIWAN is prevented and treated application in the high altitude anoxia medicine in preparation
Technical field
The invention belongs to field of medicaments, be specifically related to the application of QISHEN YIQI DIWAN in preparation prevention and treatment high altitude anoxia medicine.
Background technology
The change of aspects such as the function of human body that the anoxia in the altitude environment due to the oxygen supply deficiency can cause, metabolism and form, but threat to life when serious.Common people step into altitude environment for the first time, the adaptability compensation response of each organ of human body and tissue at first can occur, accelerate as exaggerated respiration, and increased heart rate, vasoconstrictions such as skin gastrointestinal kidney, and cerebrovascular and crown arteriectasia, or the like.Need by the above-mentioned compensatory homergy that still can not keep human body, just need treatments such as artificial oxygen uptake.If can't correct its anoxybiotic words, will make each nerve centre enter inhibitory state because of central nervous system's anoxia, feasible breathing shoals, slack-off, heart rate reduces and kinemic decline, pulmonary edema, monge disease, myocardial infarction, cerebral thrombosis, cerebral edema, in addition dead.The high altitude anoxia most people can be regulated by self and adapt to gradually, have only the sub-fraction people can produce a series of clinical manifestation.However, high altitude anoxia is prevented and treated still seem extremely important early.
QISHEN YIQI DIWAN is made up of the Radix Astragali, Radix Salviae Miltiorrhizae, Radix Notoginseng and Lignum Dalbergiae Odoriferae, in order to the angina pectoris of treatment coronary heart disease with qi deficiency and blood stasis.Chinese patent CN1375316A discloses composition, method for making and the purposes aspect angina pectoris of said preparation, but this medicine does not appear in the newspapers as yet in the effect that prevents and treats aspect the high altitude anoxia.
Summary of the invention
The purpose of this invention is to provide QISHEN YIQI DIWAN and prevent and treat application in the high altitude anoxia medicine in preparation.
QISHEN YIQI DIWAN is to be prepared from by following component by weight percentage: the Radix Astragali 22.2%~66.8%, Radix Salviae Miltiorrhizae 11.6%~33.4%, Radix Notoginseng 2.5%~13.5%, Lignum Dalbergiae Odoriferae 14.5%~44.3%.Its best proportioning is the Radix Astragali 44.7%, Radix Salviae Miltiorrhizae 26.7%, Radix Notoginseng 6.3%, Lignum Dalbergiae Odoriferae 22.3%; Perhaps with the Radix Astragali 41.2%, Radix Salviae Miltiorrhizae 23.8%, Radix Notoginseng 4.5%, Lignum Dalbergiae Odoriferae 30.5%.
For a better understanding of the present invention, below by the experiment of QISHEN YIQI DIWAN, illustrate that QISHEN YIQI DIWAN is in the effect that prevents and treats aspect the high altitude anoxia to the influence of malonaldehyde (MDA), lactic acid content in simulation 7500 meters hypoxia rat cerebral tissues of height above sea level and the blood.
One, method
1. animal is handled: 30 of (1) SD rats, body weight 180~200g is divided into normal oxygen matched group (n=10), acute hypoxia group (n=10), QISHEN YIQI DIWAN at random and adds hypoxia group (n=10).QISHEN YIQI DIWAN adds hypoxia group by 0.8ml/100g body weight (be 30mg QISHEN YIQI DIWAN/100g body weight, use water dissolution) dosage gastric infusion every day 1 time, continuous 7 days; All the other two groups give isopyknic water.(2) decompression method: acute hypoxia group and QISHEN YIQI DIWAN add the hypoxia group animal and placed in the lower pressure environment tank in 1 hour after the administration the last time, rise to 7500 meters of height above sea level with the speed of 10 meter per seconds, continue 7 hours; Normal oxygen matched group is left intact, and normal oxygen room temperature is raised.Behind the deliver from vault each treated animal break end rapidly the stripping brain, get blood.Wherein half brain is used for the mensuration of brain water content, makes 5% brain homogenate by the normal saline of 0~4 ℃ of 1: 20 (w/v) adding after half brain is weighed in addition.Centrifugal blood is got serum.
2. brain water content is measured: with doing wet mass method, each is organized half side cerebral tissue and places weighing botle, weighs to be placed on 50 ℃ of stove-dryings to constant weight.By formula calculate brain water content: brain water content (%)=(wet weight-dry weight)/wet weight * 100%.
3. biochemical measurement: measure the brain homogenate protein content with Folin-phenol method.Get 5% brain homogenate, each 100 μ l mensuration MDA content of serum; MDA content is represented with nmol/mgprot in the tissue, and MDA content is represented with nmol/ml in the serum.Get 5% brain homogenate, each 10 μ l mensuration lactic acid content of serum; Lactic acid content represents that with μ mol/mgprot lactic acid content is represented with mmol/L in the serum in the tissue.
4. statistical method: every group of experimental result represent with x ± s, respectively organizes the significance of difference between the mean with the t check.
Two, result
1. the variation of brain tissue homogenate's water content, MDA and lactic acid content (seeing Table 1)
The normal oxygen matched group of acute hypoxia group brain water content significantly increases (P<0.01), and MDA and lactic acid content obviously increase (P<0.01); Brain water content, MDA and lactic acid content that QISHEN YIQI DIWAN adds hypoxia group obviously reduce (P<0.05 or P<0.01) than the acute hypoxia group.
The variation of table 1 brain tissue homogenate water content, MDA, lactic acid content (x ± s)
Group | Water content (%) | MDA(nmol/mgprot) | Lactic acid (μ mol/mgprot) |
Normal oxygen matched group | 78.06±0.192 | 6.042±0.845 | 0.155±0.034 |
The acute hypoxia group | 78.71±0.221
** | 7.483±0.747
** | 0.239±0.041
** |
QISHEN YIQI DIWAN adds hypoxia group | 78.38±0.281
**## | 6.753±0.426
*# | 0.178±0.039
## |
Annotate: compare with normal oxygen matched group,
*P<0.05
*P<0.01
Compare with the acute hypoxia group,
#P<0.05
##P<0.01
2. the variation of MDA and lactic acid content in the serum (seeing Table 2)
The normal oxygen matched group of MDA and lactic acid content significantly increases (P<0.01) in the acute hypoxia group serum, and QISHEN YIQI DIWAN adds that MDA and lactic acid content significantly reduce (P<0.01) than the acute hypoxia group in the hypoxia group serum.
The variation of MDA, lactic acid content in table 2 serum (x ± s)
Group | MDA(nmol/ml) | Lactic acid (mmol/L) |
Normal oxygen matched group | 7.562±0.985 | 9.414±1.122 |
The acute hypoxia group | 10.138±1.241
** | 10.987±1.124
** |
QISHEN YIQI DIWAN adds hypoxia group | 7.112±0.973
## | 8.763±1.257
## |
Annotate: compare with normal oxygen matched group,
*P<0.05
*P<0.01
Compare with the acute hypoxia group,
#P<0.05
##P<0.01
Hypoxia causes that the process of glucose anerobic glycolysis in cerebral tissue and the blood strengthens, and lactic acid generates to be increased.Lactic acid gathers at tissue and can cause Premeabilisation of cells to be pressed increasing, and mitochondrial swelling and breaking influences the mitochondrial respiratory function and energy metabolism takes place falls and hinder.Lactic acid and oxygen-derived free radicals and lipid peroxide generation increase in the born of the same parents can damage mitochondrial energy metabolism again during hypoxia, and the ATP level descends in the born of the same parents.In the above-mentioned experiment, lactic acid and MDA content obviously increase in rat cerebral tissue and the blood after exposed hypoxia, and give in advance before the hypoxia that QISHEN YIQI DIWAN can suppress lactic acid and MDA content increases.This result shows, and QISHEN YIQI DIWAN has the ATP of promotion generative capacity, can be used for preventing and treating high altitude anoxia.
The specific embodiment
Below in conjunction with embodiment medicine of the present invention is described further, following each embodiment only is used to the present invention is described and is not limitation of the present invention.
Embodiment one
Get Radix Astragali 86.5g, Radix Salviae Miltiorrhizae 21.3g, Radix Notoginseng 3.5g, Lignum Dalbergiae Odoriferae 20.6g, adjuvant Polyethylene Glycol-6000 30g.Will be through Radix Salviae Miltiorrhizae, the Radix Notoginseng of pulverizing, decocting boils 2 times, adds 7 times of water gagings at every turn, each 2 hours, merge decoction liquor, filter, filtrate is concentrated into 900ml, add 95% ethanol, make determining alcohol reach 70%, left standstill 12~24 hours, filter, reclaim ethanol, being condensed into relative density is the extractum of 1.32~1.38 (50~60 ℃); To decoct with water 2 times through the Radix Astragali of pulverizing, add 6 times of water gagings at every turn, extracted successively 2 hours, 1 hour, merging filtrate is when being concentrated into the 1500ml left and right sides, adding 95% ethanol, to make determining alcohol be 60%, left standstill 12~24 hours, filters, filtrate recycling ethanol, when being concentrated into the 400ml left and right sides, adding 95% ethanol, to make determining alcohol be 80%, left standstill 12~24 hours, filter, filtrate recycling ethanol, being condensed into relative density is the extractum of 1.32~1.38 (50~60 ℃); Get Lignum Dalbergiae Odoriferae, add 5 times of water gagings, reflux, extract, 5 hours is collected volatile oil; Get above-mentioned Salvia miltiorrhiza and Panax notoginseng extractum, Radix Astragali extractum and Polyethylene Glycol-6000, water-bath is dissolved, and after change is even, adds volatile oil of Lignum Dalbergiae Odoriferae, and mixing moves in the drop pill machine, makes 1000 drop pill.
Embodiment two
Get Radix Astragali 40.6g, Radix Salviae Miltiorrhizae 44.8g, Radix Notoginseng 11.2g, Lignum Dalbergiae Odoriferae 38.6g, adjuvant Polyethylene Glycol-6000 30g makes Chinese medicine preparation of the present invention by the preparation technology of embodiment 1.
Embodiment three
Get Radix Astragali 77.3g, Radix Salviae Miltiorrhizae 22.8g, Radix Notoginseng 4.8g, Lignum Dalbergiae Odoriferae 30.5g, adjuvant Polyethylene Glycol-6000 28g, make Chinese medicine preparation of the present invention by the preparation technology of embodiment 1.
Embodiment four
Get Radix Astragali 42.3g, Radix Salviae Miltiorrhizae 39.2g, Radix Notoginseng 8.2g, Lignum Dalbergiae Odoriferae 46.8g, adjuvant Polyethylene Glycol-6000 25g, make Chinese medicine preparation of the present invention by the preparation technology of embodiment 1.
Embodiment five
Get Radix Astragali 65.2g, Radix Salviae Miltiorrhizae 38.9g, Radix Notoginseng 9.3g, Lignum Dalbergiae Odoriferae 32.5g, adjuvant Polyethylene Glycol-6000 40g, make Chinese medicine preparation of the present invention by the preparation technology of embodiment 1.
Embodiment six
Get Radix Astragali 56.2g, Radix Salviae Miltiorrhizae 32.5g, Radix Notoginseng 6.2g, Lignum Dalbergiae Odoriferae 41.6g, adjuvant Polyethylene Glycol-6000 22g, make Chinese medicine preparation of the present invention by the preparation technology of embodiment 1.
Embodiment seven
Get Radix Astragali 36.5g, Radix Salviae Miltiorrhizae 32.4g, Radix Notoginseng 6.2g, Lignum Dalbergiae Odoriferae 41.7g, adjuvant Polyethylene Glycol-6000 22g, make Chinese medicine preparation of the present invention by the preparation technology of embodiment 1.
Embodiment eight
Get Radix Astragali 65.6g, Radix Salviae Miltiorrhizae 25.8g, Radix Notoginseng 9.5g, Lignum Dalbergiae Odoriferae 46.4g, adjuvant Polyethylene Glycol-6000 35g makes Chinese medicine preparation of the present invention by the preparation technology of embodiment 1.
Embodiment nine
Get Radix Astragali 35.5g, Radix Salviae Miltiorrhizae 50.8g, Radix Notoginseng 16.3g, Lignum Dalbergiae Odoriferae 52.3g, adjuvant Polyethylene Glycol-6000 35g, make Chinese medicine preparation of the present invention by the preparation technology of embodiment 1.