CN1401365A - Chinese health medicine - Google Patents
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- CN1401365A CN1401365A CN 02131251 CN02131251A CN1401365A CN 1401365 A CN1401365 A CN 1401365A CN 02131251 CN02131251 CN 02131251 CN 02131251 A CN02131251 A CN 02131251A CN 1401365 A CN1401365 A CN 1401365A
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Abstract
A health-care Chinese medicine for resisting against radiation and regulating immunity is prepared from proanthocyanidin and American ginseng or ginseng in a ratio of 1:(0.01-100).
Description
Technical field
The present invention relates to a kind of health medicine or food, particularly with Radix Panacis Quinquefolii or Radix Ginseng and procyanidin (Semen Vitis viniferae or Cortex Pini Massonianae extract) health medicine or food compositions as active component.
Background technology
Radix Panacis Quinquefolii is the araliaceae ginseng plant, has the supplementing QI and nourishing YIN characteristics, and tradition is mainly used in Chinese medical discrimination and is the deficiency syndrome patient of " deficiency of both QI and YIN " and the clinical symptoms that is produced.
Radix Ginseng is the Araliaceae herbaceos perennial.Root is used as medicine.Sweet, the little hardship of nature and flavor, temperature.Effect is a strongly invigorating primordial QI, invigorating the spleen to benefit the lung, promoting the production of body fluid to quench thirst, intelligence promoting and the mind tranquilizing.Be used for the deficiency of vital energy and desire to take off disease, deficiency of spleen-QI, lung qi lose empty, and Tianjin wound is thirsty, quench one's thirst.
Contain similar ingredient ginsenoside in Radix Ginseng and the Radix Panacis Quinquefolii.
Cortex Pini is the Pinaceae platymiscium, has the advantages that to drive wind prevailing over dampness, blood stasis dispelling, and tradition is mainly used in treatment of diseases such as rheumatic osteopathia, traumatic injury.Contain procyanidin in the Cortex Pini Massonianae extract, be a kind of natural antioxidant, free radical scavenger and UV absorbent have the cardio and vascular function of improvement efficiently; Blood pressure lowering; Blood fat reducing; Anti-allergic effects; Also can be used as sun-proof host in the cosmetics.
Also existing in the Semen Vitis viniferae in the human body can not synthetic natural materials, procyanidin, and it can effectively remove unnecessary free radical in the body, and oxidation resistance is 50 times of vitamin E, ascorbic 20 times.At present, its oxidation resistance has obtained internationally recognized.
Contain common composition procyanidin in Cortex Pini and the Semen Vitis viniferae.
At present Songyuan City's anthocyanidin and Radix Ginseng or Radix Panacis Quinquefolii are not share the report of making the medicines and health protection product.
The surprised discovery of the present invention has procyanidin and Radix Ginseng or Radix Panacis Quinquefolii use in conjunction the effect of better radioprotective, mediator's body immunity function, both additions are used has collaborative the work, and the procyanidin source fully, cheap, can buy, medicine of the present invention or health product have good health protection and therapeutic action.
Summary of the invention
The invention provides the medicine or the health product of Radix Panacis Quinquefolii or Radix Ginseng and procyanidin Application of composite, this medicine or health product can be used for radioprotective, mediator's body immunity function, have disease preventing and treating, the health effect.
Medicine of the present invention or health product, effective ingredient is, procyanidin (is mainly extracted by Semen Vitis viniferae or Cortex Pini, can directly buy and obtain, also can from other medicinal plants that are rich in procyanidin, extract acquisition, can be pure product, it also can be crude product, content is high more good more) and the crude drug raw material or the compound composition of extract of Radix Panacis Quinquefolii or Radix Ginseng, the part by weight of its composition is: the weight ratio of procyanidin and Radix Panacis Quinquefolii or Radix Ginseng is 1: 0.01-100, the Radix Panacis Quinquefolii wherein or the amount of Radix Ginseng can be the amounts of extract, it also can be the amount of crude drug raw material, the weight ratio of preferred procyanidin and Radix Panacis Quinquefolii or Radix Ginseng is 1: 0.1-10, wherein the extract of Radix Panacis Quinquefolii or Radix Ginseng through water or/and alcohol extraction, concentrate, drying steps is made, the crude drug raw material is Radix Panacis Quinquefolii or Radix Ginseng drying, pulverizing is made, procyanidin wherein be by Semen Vitis viniferae or Cortex Pini through water or/and alcohol extraction, concentrate, drying is made.The amount of preferred procyanidin is the 1-2 weight portion, the amount of Radix Panacis Quinquefolii or Radix Ginseng is the 2-6 weight portion, wherein the weight of effective ingredient also can be calculated by crude drug, preferred proportion is calculated as by crude drug, Semen Vitis viniferae or Cortex Pini: Radix Ginseng or Radix Panacis Quinquefolii=5-20: 1, this medicine or health product also can add physiologically acceptable carrier when needed.
The present invention also comprises, the application in the medicine of a kind of radioprotective of preparation and mediator's body immunity function of medicine of the present invention or health product.
Medicine of the present invention or health product, described physiologically acceptable carrier can be the various materials of oral or external preparation, as: starch, sucrose, lactose, cellulose derivative, cyclodextrin, beta-schardinger dextrin-, phospholipid material, magnesium stearate etc.
Medicine of the present invention or health product, can make different dosage forms, peroral dosage form preferably, these dosage forms can be, tablet, capsule, oral liquid, suck agent, granule, electuary, pill, powder, suspensoid, powder, solution, injection, suppository, ointment, plaster, cream, spray, drop, patch.
Medicine of the present invention or health product, effective ingredient procyanidin wherein and Radix Panacis Quinquefolii or Radix Ginseng can extract by common Chinese medicine extraction method, carry or alcohol extraction as water, water precipitating or precipitate with ethanol, extracting solution through concentrating, dry, make extractum, but pharmacy after the pulverizing, Radix Ginseng in medicine of the present invention or the health product or Radix Panacis Quinquefolii are made up a prescription after also can directly pulverizing and making Radix Ginseng powder or Radix Panacis Quinquefolii powder, need not to extract.With effective ingredient with make preparation according to a conventional method after physiologically acceptable carrier mixes and promptly can be made into medicine of the present invention or health product.
Specifically, the extraction of medicine of the present invention or health product effective ingredient procyanidin can be adopted following method, can adopt ethanol to boil for the extraction of procyanidin in the Cortex Pini carries, concentration of alcohol is 10-95%, separate and adopt water precipitating, alcohol precipitating method to separate respectively, water is that 1-10 doubly measures, and concentration of alcohol is 70-95%, and consumption is that 1-10 doubly measures.Semen Vitis viniferae extract also can adopt same or the similar approach preparation.Below be technological process:
Radix Panacis Quinquefolii is measured alcohol reflux three times for 8 times, and filtrate concentrates, and reclaims ethanol, gets thick paste, and drying under reduced pressure is pulverized, and 80 orders sieve, and get extract powder 1.
6 times of amounts of Cortex Pini alcohol reflux three times, filtrate concentrates, and gets thick paste, and water precipitating filters, concentrates, and 85% precipitate with ethanol filters, and drying under reduced pressure is pulverized, and 80 orders sieve, and get extract powder 2.
Extract powder 1, extract powder 2, lactose, the magnesium stearate mix homogeneously, encapsulated, through quality inspection, pack finished product.
Same method can be made into the compound product of compound product, Radix Ginseng and Cortex Pini Massonianae extract of Radix Ginseng and Semen Vitis viniferae extract and the compound product of Radix Panacis Quinquefolii and Semen Vitis viniferae extract.
Medicine of the present invention or health product, its effective ingredient procyanidin and Radix Ginseng or Radix Panacis Quinquefolii extract also can have been bought from the market.The raw material of buying can be made into medicine of the present invention or health product through processing.
The present invention is capsule most preferably, and every capsules contains procyanidin 50-100mg, Radix Panacis Quinquefolii or Radix Ginseng 100-200mg.Specifically be that every capsules contains procyanidin 75mg, Radix Panacis Quinquefolii or Radix Ginseng 150mg, medicine acceptable carrier 76.5mg.
The preparation method of medicine of the present invention or health product is with procyanidin and Radix Panacis Quinquefolii or Radix Ginseng and the mixing of physiologically acceptable carrier.
Medicine of the present invention or health product have fabulous radioprotective and immunoregulation effect through experiment confirm, below are the experiment illustration of medicine of the present invention or health product.
Experimental example one: the immunoregulation effect trial target is for pressing the capsule of embodiment 1 preparation
Experimental technique: internal organs/weight ratio pH-value determination pH continuous irrigation stomach is after 25 days, cervical vertebra takes off puts to death animal, takes out spleen, thymus, and analytical balance weighs up animal, spleen, thymic weight respectively, calculate internal organs/body weight ratio (representing) by fraction, and carry out statistical procedures with mg/10g.
Delayed allergy (the sufficient sole of the foot thickens method) continuous irrigation stomach is after 20 days, and every mouse peritoneal injection 2%SRBC0.2ml immunity continued to irritate stomach after 4 days, measured left back sufficient sole of the foot thickness, measured and averaged for 3 times; Then at measuring point subcutaneous injection 20% (v/v) SRBC, every 20 μ l.Inject and measured left back sufficient sole of the foot thickness in back 24 hours, 48 hours respectively, measure and average for 3 times.Calculate the difference of sufficient sole of the foot degree of capturing before and after attacking, represent the degree of mouse DTH with difference, and carry out statistical procedures.
The serum amboceptor is measured (blood clotting method) continuous irrigation stomach 20 days, every mouse peritoneal injection 2%SRBC0.The 2ml immunity continued to irritate stomach after 5 days, extractd eyeball and got blood in centrifuge tube, placed 1 hour, peel off, centrifugal 10 minutes of 2000rpm collects serum, with normal saline serum is made doubling dilution, every part of dilution 12 holes place blood-coagulation-board with the dilution serum of difference, and every hole 100 μ l add 0 again.5%SRBC suspension 100 μ l, mixing is put 37 ℃ in wet box observed result after 3 hours, writes down the coagulation degree in every hole.Calculating antibody product by formula, and carry out statistical procedures.
Turnover of Mouse Peritoneal Macrophages was engulfed chicken red blood cell experiment (half intracorporal method) continuous irrigation stomach after 25 days, inject 20% chicken erythrocyte suspension 1ml in every mouse peritoneal, after 30 minutes, the cervical vertebra dislocation is put to death, be fixed on the Mus plate, abdominal skin is cut off in the center, injects normal saline 2ml through the abdominal cavity, rotated the Mus plate 1 minute, sucking-off abdominal cavity washing liquid 1ml, mean droplet is carried on the sloping sheet in 2, puts 37 ℃ in wet box 30 minutes, taking-up is rinsing in normal saline, dry, fixing, 4%GiemsaPBS dyeing 3 minutes, the distilled water rinsing is dried, microscopy.Press fraction and calculate phagocytic percentage and phagocytic index, and carry out statistical procedures.
The NK cytoactive was measured (determination of lactate dehydrogenase method) continuous irrigation stomach after 25 days, and animal is put to death in the cervical vertebra dislocation, takes out spleen, tears up, cross 200 eye mesh screens after, use Hank ' s3 time, be made into 5 * 10 with complete RPMI-1640
6Individual/ml cell suspension. the cell suspension of each mice is got 300 μ l be placed in 96 well culture plates, every hole 100 μ l, every hole adds target cell (YAC-1 cell, 1 * 10
5Individual/ml) 100 μ l, do target cell nature release aperture (target cell 100 μ l+ culture fluid 100 μ l) and each 8 hole of maximum release aperture (target cell 100 μ l+1%NP-40 100 μ l) simultaneously, 37 ℃ of 5%CO
2Cultivated 4 hours, took out 1500rpm centrifugal 5 minutes.Each hole supernatant 100 μ l is placed another culture plate, and every hole adds 100 μ l substrate liquid again, adds 1mol/L HCl 30 μ l cessation reactions after 10 minutes, measures the 0.D. value at the 490nm place, by formula calculates NK cytoactive rate, and carries out statistical procedures.The results are shown in following table:
The influence of table 1. pair mice body weight (serum hemolysin and delayed allergy mensuration group) the initial body weight of group number of animals (g) body weight in mid-term (g) end body weight (g) weightening finish value (g) p value (only) (-
x± S) ± S--control group 11 19.84 ± 0.83 20.95 ± 0.81 21.68 ± 0.78 1.84 ± 0.3537.5mg/kg.bw 11 19.64 ± 0.75 20.69 ± 0.85 21.47 ± 0.98 1.83 ± 0.40＞0.0575.0mg/kg.bw organizes 12 19.66 ± 0.92 20.96 ± 0.86 21.55 ± 0.67 1.89 ± 0.49＞0.05150.0mg/kg.bw 12 19.78 ± 0.97 20.83 ± 0.60 21.65 ± 0.62 1.87 ± 0.64＞0.05
Table 2. finishes body weight (g) weightening finish value (g) p value control group 12 19.95 ± 0.78 21.28 ± 1.01 21.28 ± 1.01 1.88 ± 0.87-37.5mg/kg.bw 11 19.62 ± 0.88 21.13 ± 0..96 21.56 ± 1.00 1.94 ± 0.65＞0.0575.0mg/kg.bw 12 19.94 ± 0.93 21.25 ± 0.84 21.83 ± 0.55 1.89 ± 0.61＞0.05150.0mg/kg.bw 11 19.74 ± 0.79 21.06 ± 0.91 21.57 ± 0.85 1.83 ± 0.67＞0.05 to the impact initial body weight of (engulfing the chicken red blood cell test group) group number of animals (g) body weight in mid-term (g) of Mouse Weight
Table 3. finishes body weight (g) weightening finish value (g) p value control group 12 19.60 ± 0.24 20.67 ± 1.22 21.35 ± 1.30 1.75 ± 0.35-37.5mg/kg.bw 11 19.62 ± 0.70 20.70 ± 0.97 21.28 ± 1.88 1.66 ± 0.56＞0.0575.0mg/kg.bw 11 19.77 ± 0.82 20.71 ± 0.79 21.60 ± 0.75 1.83 ± 0.62＞0.05150.0mg/kg.bw 12 19.76 ± 0.80 20.86 ± 0.81 21.39 ± 0.94 1.64 ± 0.54＞0.05* to impact (NK cytoactive detection group) the initial body weight of group number of animals (g) body weight in mid-term (g) of Mouse Weight and represents the weightening finish value comparison of each dosage group and control group
Table 4. pair mice internal organs/body weight ratio influence group number of animals spleen/body weight ratio P value * thymus/weight ratio P value *
(only), (mg/10g) matched group 11 48.72 ± 0.98-11.89 ± 2.32-37.5mg/kg.bw 11 51.45 ± 7.07＞0.05 14.00 ± 2.49＞0.0575.0mg/kg.bw organizes 12 51.63 ± 5.53＞0.05 13.18 ± 2.01＞0.05150.0mg/kg.bw 12 49.75 ± 6.79＞0.05 12.65 ± 2.34＞0.05*P
1Represent each dosage group and matched group spleen/body weight ratio relatively, P
2Be worth the relatively influence (table 5) of 2.3 pairs of mice delayed allergies (DTH) of each dosage group and matched group thymus/body weight ratio
Antigen was attacked after 24 hours, 37.5mg/kg.bw dosage is compared with control group mice, swelling degree of the paw does not have significance and increases (P＞0.05), 75.0mg/kg.bw dosage 150.0mg/kg.bw dosage compare swelling degree of the paw there are no significant difference (P＞0.05) with control group mice.Table 5. pair mice delayed allergy (DTH) influence group number of animals (only)
Swelling degree of the paw (mm)P value * P value *
24h 48h control group 11 0.26 ± 0.10 0.18 ± 0.10--37.5mg/kg.bw 11 0.27 ± 0.12 0.18 ± 0.11＞0.05＞0.0575.0mg/kg.bw organizes 12 0.40 ± 0.11 0.18 ± 0.10＜0.05＞0.05150.0mg/kg.bw 12 0.37 ± 0.11 0.19 ± 0.09＜0.05＞0.05*P1Represent 24 hours swelling degree of the paw of each dosage group and matched group relatively, P
2Be worth 48 hours swelling degree of the paw of each dosage group and matched group relatively
Table 6. pair mice serum hemolytic antibody product influence the group number of animals, (only) antibody product, (P value * matched group 11 181.27 ± 10.23-37.5mg/kg.bw 11 180.45 ± 9.18＞0.0575.0mg/kg.bw organizes 12 181.17 ± 12.16＞0.05150.0mg/kg.bw, 12 198.00 ± 15.67＜0.01*P and represents each dosage group and matched group antibody product relatively
What table 7. pair Turnover of Mouse Peritoneal Macrophages was engulfed the chicken red blood cell ability influences group number of animals phagocytic percentage (%) P
1Value * phagocytic index P
2Value * (only) control group 12 28.33 ± 6.22-0.99 ± 0.20-37.5mg/kg.bw 11 35.27 ± 9.03＜0.05 1.42 ± 0.27＜0.0175.0mg/kg.bw organize 12 35.96 ± 6.52＜0.05 1.39 ± 0.28＜0.01150.0mg/kg.bw 11 37.00 ± 7.86＜0.05 1.53 ± 0.26＜0.01*P1Represent each dosage group and matched group phagocytic percentage relatively, P
2Be worth each dosage group and matched group phagocytic index relatively
2.5 to each dosage group of the active influence of NK cells in mice (table 8) respectively with control group mice group ratio, its NK cytoactive rate there are no significant difference (P＞0.05).
Group number of animals (only) the NK cytoactive rate (%) () that the affects P value * control group 12 9.19 ± 6.20 of table 8. pair NK cells in mice activity--37.5mg/kg.bw 11 13.80 ± 8.42＞0.0575.0mg/kg.bw organizes 11 12.76 ± 6.67＞0.05150.0mg/kg.bw, 11 13.18 ± 6.84＞0.05*P table not each dosage group and the comparison of control group NK cytoactive rate
Experimental example two: radiation resistance experimental technique
1. select female Kunming mouse, be divided into blank group, radiation matched group and three test group at random.The test group animal respectively by recommended dose 450mg/ people/order (7.5mg/kg..bw) doubly, 20 times, 30 times (75mg/kg..bw, 225gm/kg.bw) irritate stomach every day once, totally 30 days, blank group, radiation control animals were irritated stomach with distilled water every day.The 3rd week was used radiation matched group and three test group animals after irritating stomach
60The Cor ray carries out irradiation, once a day, 1.0GY/ time, totally 10 times.
2. got blood in 3 days behind the last irradiation and put to death a collection of zoometry serum superoxide dismutases (SOD) active (the yellow fast oxidation enzyme process of holding in the mouth or the eyes), peripheral white blood cell (WBC), get bone marrow of sternum by micronucleus test prescriptive procedure film-making in " toxicological evaluation of food safety procedure and method ", dyeing, microscopy; Another batch animal gives over to observes for the first time behind the irradiation 30 days survival rates and mean survival time.The results are shown in following table (test group is to give the medicament of the method manufacturing of the embodiment of the invention 1, is called the eyelash curing capsule):
Table 1 eyelash curing capsule is to the influence of mice body weight
Initial body weight body weight in mid-term finishes (mg/kg..bw) number of animals body weight number of animals body weight number of animals body weight of body weight group dosage------
(only) (g) (only) (g) (only) (g) blank 0 30 21.0 ± 1.1 30 28.0 ± 1.7 29 31.4 ± 2.5 radiation contrast 0 30 20.9 ± 1.1 30 28.3 ± 3.0 20 26.3 ± 4.3
*Eyelash curing capsule 75 30 20.9 ± 1.1 30 27.8 ± 2.4 29 26.5 ± 3.4*
150 30 20.9±1.0 30 27.0±2.5 16 24.3±3.9*
225 30 20.9±1.1 30 27.0±2.6 20 25.8±3.4*
Last irradiation rear section animal dead.*: p＜0.01 (with the blank group relatively) body weight all significantly was lower than blank group (p＜0.01) when radiation matched group and three test dose group mices were finished, and did not have significant difference between three eyelash curing capsule dosage groups and the radiation matched group.
Table 2 pair mice peripheral blood cells number influence group dosage number of animals leukocyte count (mg/kg.bw) (only) (X10
9/ L) blank 0 12 7.94 ± 1.61 radiation contrast 0 12 0.41 ± 0.28
* ▲Eyelash curing capsule 75 12 0.99 ± 0.23
* ▲
150 12 0.66±0.46
*▲
225 12 0.91 ± 0.46
* ▲ *: p＜0.01 (comparing) with the blank group:
▲:P＜0.01 (with the radiation matched group relatively) be subjected to irradiation respectively organize the mice peripheral white blood cell all significantly be lower than the blank group (
:P＜0.01), three dosage groups of eyelash curing capsule murine interleukin digital display work be higher than the radiation matched group (
:P＜0.01), promptly the eyelash curing capsule has the rising effect to raying murine interleukin number.
Irradiation mice superoxide dismutase (SOD) is active to be influenced 0 11 486.43 ± 58.02 radiation of group dosage number of animals leukocyte count (mg/kg.bw) (only) (NU/ml serum) blank and contrasts 0 11 389.71 ± 50.37 table 3 eyelash curing capsule to being subjected to
*Eyelash curing capsule 75 11 411.93 ± 58.83
*
150 11 447.00±36.60
▲
225 11 462.25 ± 42.88
▲:
:P＜0.01 (comparing) with the blank group:
▲::
:P＜0.01 (with the radiation matched group relatively) radiation matched group, eyelash curing capsule 75mg/kg.bw dosage group mice serum superoxide dismutase activity significantly be lower than the blank group (
:P＜0.01), 150mg/kg.bw, 225mg/kg.bw dosage group mice superoxide dismutase activity be significantly higher than the radiation matched group (
:P＜0.05), promptly the eyelash curing capsule can increase and is subjected to irradiation mice serum superoxide dismutase activity.
Table 4 eyelash curing capsule influences group dosage number of animals mean survival time to what be subjected to irradiation mice mean survival time
(mg/kg..bw) (only) (my god) blank 0 15 29.0 ± 3.9 radiation contrast 0 15 12.1 ± 3.5
*Eyelash curing capsule 75 15 21.8 ± 4.7
* ▲
150 15 13.9±1.6
225 15 13.5±4.8
P＜0.01 (comparing) with the blank group:
▲(compare with the radiation matched group p＜0.01
Annotate: the time-to-live is the 1st the irradiation number day after tomorrow in the table, and the dead animals survived time was in behind the 1st irradiation 30 days.
Radiation matched group and three dosage groups of eyelash curing capsule mice be subjected to the mean survival time behind the irradiation significantly be shorter than the blank group (
:P＜0.01), 75mg/kg.bw dosage group mice is subjected to the mean survival time behind the irradiation significantly to be longer than radiation matched group p＜0.01) be that the eyelash curing capsule can increase the mean survival time that is subjected to the irradiation mice.
Table 5 eyelash spirit glue is subjected to behind the irradiation group dosage that influences of 30 days survival rates to observe 30 days surviving animals of number of animals and count survival rate to mice
(mg/kg..bw) (only) (only) (%) blank 0 15 14 93.3 radiation contrast 0 15 0 0.0* eyelash curing capsules 75 15 2 13.3
*
150 15 0 0.0
*
225 15 0 0.0
*
P＜0.01 (with the blank group relatively): annotate: survival rate is 30 days a survival rate behind the 1st irradiation in the table.Be subjected to irradiation respectively to organize mice 30 days survival rates after being subjected to irradiation for the first time and all significantly be lower than blank group (p＜0.01), three dosage groups of eyelash curing capsule mice is subjected to behind the irradiation 30 days survival rates and radiation matched group not to have significant difference (p＜0.05), and promptly the eyelash curing capsule does not have the increase effect to 30 days survival rates of raying mice.The eyelash curing capsule is to the influence of mouse Bone marrow cells micronucleus rate
Be subjected to irradiation respectively to organize the PCEMNR micronuclear rates and all be significantly higher than blank group (p＜0.01), three dosage groups of eyelash curing capsule PCEMNR micronuclear rates significantly is lower than radiation matched group (p＜0.01), and dose-response relationship is preferably arranged.Be that the eyelash curing capsule has the effect (seeing Table 6) that reduces raying PCEMNR micronuclear rates
Table 6 eyelash curing capsule is had a liking for many RBC number micronucleus and is counted micronuclear rates the group dosage number of animals that influences of mouse Bone marrow cells micronucleus rate
(mg/kg.bw) (only) (individual/only) (individual) (%
0) blank 0 10 1,000 1.5 ± 0.8 1.5 radiation contrast 0 10 1,000 27.8 ± 4.3*, 27.8 eyelash curing capsules, 75 10 1,000 20.9 ± 2.6*
▲20.9
150 10 1000 14.6±2.0*
▲ 14.6
225 10 1,000 8.2 ± 1.2*
▲8.2
*: p＜0.01 (comparing) with the blank group,
▲P＜0.01 (with the radiation matched group relatively) with the safety evaluatio that the health medicine compositions of this product invention is carried out, the result is as follows: acute toxicity test: the anxious poisons mouth LD of large and small Mus all greater than 10000mg/kg.bw, is declared true border non-toxic type; Salmonella reversion test, mouse marrow cell micro nuclear test and three genetic toxicity tests of mouse sperm deformity test are negative findings, promptly do not have mutagenic action.Long term toxicity test: get 50 of rat, be equivalent to 100 times of people's dosage by 750mg/kg.bw), continuously the port gastric infusion is 90 days, and is all not unusual, puts to death also no abnormality seen of internal organs pathology section examination such as the internal organs perusal of basic animal behind the animal and the heart, liver, spleen, lung kidney.The result shows medicine avirulence of the present invention.Other the product of prescription of the present invention also has the same function in the above-mentioned experiment, and is very little as their toxicity, all has immunomodulating and radiation protection, all can be by oral, and every day three times, each 1-5 capsules.
The present invention is described further by the following examples for the specific embodiment:
Embodiment 1 Cortex Pini 1500g Radix Panacis Quinquefolii 150g gets 75% alcohol reflux three times that Cortex Pini adds 6 times of amounts, each 2 hours, merging filtrate; Reclaim ethanol, concentrated filtrate is to the thick paste shape, and 5 times of water gagings were placed 24 hours, filtering and concentrating, and 4 times of amount 85% ethanol were placed 24 hours, filtered, and drying under reduced pressure is pulverized extractum, crosses 80 mesh sieves; Get 75% alcohol reflux three times of participating in 8 times of amounts in the West, each 2 hours, merging filtrate; Reclaim ethanol, concentrated filtrate is to thick paste shape, drying under reduced pressure; Pulverize extractum, cross 80 mesh sieves; Merge two medicines, add lactose 75g and magnesium stearate 1.5g, mix homogeneously; Adorn 1000 capsules, quality inspection, packing.
Embodiment 2 Semen Vitis viniferae 1500g Radix Ginseng 150g get 75% alcohol reflux three times that Semen Vitis viniferae adds 6 times of amounts, each 2 hours, merging filtrate; Reclaim ethanol, concentrated filtrate is to the thick paste shape, and 5 times of water gagings were placed 24 hours, filtering and concentrating, and 4 times of amount 85% ethanol were placed 24 hours, filtered, and drying under reduced pressure is pulverized extractum, crosses 80 mesh sieves; Get 75% alcohol reflux three times that the people participates in 8 times of amounts, each 2 hours, merging filtrate; Reclaim ethanol, concentrated filtrate is to thick paste shape, drying under reduced pressure; Pulverize extractum, cross 80 mesh sieves; Merge two medicines, add lactose 75g and magnesium stearate 1.5g, mix homogeneously; Adorn 1000 capsules, quality inspection, packing.
Embodiment 3 Cortex Pini 1500g Radix Ginseng 150g get 75% alcohol reflux three times that Cortex Pini adds 6 times of amounts, each 2 hours, merging filtrate; Reclaim ethanol, concentrated filtrate is to the thick paste shape, and 5 times of water gagings were placed 24 hours, filtering and concentrating, and 4 times of amount 85% ethanol were placed 24 hours, filtered, and drying under reduced pressure is pulverized extractum, crosses 80 mesh sieves; Get 75% alcohol reflux three times that the people participates in 8 times of amounts, each 2 hours, merging filtrate; Reclaim ethanol, concentrated filtrate is to thick paste shape, drying under reduced pressure; Pulverize extractum, cross 80 mesh sieves; Merge two medicines, add lactose and magnesium stearate is an amount of, mix homogeneously; Adorn 1000 capsules, quality inspection, packing.
Embodiment 4 Semen Vitis viniferae 1500g Radix Panacis Quinquefolii 150g get 75% alcohol reflux three times that Semen Vitis viniferae adds 6 times of amounts, each 2 hours, merging filtrate; Reclaim ethanol, concentrated filtrate is to the thick paste shape, and 5 times of water gagings were placed 24 hours, filtering and concentrating, and 4 times of amount 85% ethanol were placed 24 hours, filtered, and drying under reduced pressure is pulverized extractum, crosses 80 mesh sieves; Get 75% alcohol reflux three times of participating in 8 times of amounts in the West, each 2 hours, merging filtrate; Reclaim ethanol, concentrated filtrate is to thick paste shape, drying under reduced pressure; Pulverize extractum, cross 80 mesh sieves; Merge two medicines, add lactose and magnesium stearate is an amount of, mix homogeneously; Adorn 1000 capsules, quality inspection, packing.
Embodiment 5 Cortex Pini Massonianae extract 75g Radix Panacis Quinquefolii extract 150g get the said extracted thing and cross 80 mesh sieves; Merge two medicines, add lactose and magnesium stearate is an amount of, mix homogeneously; Adorn 1000 capsules, quality inspection, packing.
Embodiment 6 Semen Vitis viniferae extract 75g Radix Ginseng extract 150g get the said extracted thing and cross 80 mesh sieves; Merge two medicines, add lactose and magnesium stearate is an amount of, mix homogeneously; Adorn 1000 capsules, quality inspection, packing.
Embodiment 7 procyanidin 75g Radix Ginseng extract 150g get procyanidin and Radix Ginseng extract is crossed 80 mesh sieves; Merge two medicines, add lactose and magnesium stearate is an amount of, mix homogeneously; Adorn 1000 capsules, quality inspection, packing.
Embodiment 8 procyanidin 75g Radix Panacis Quinquefolii extract 150g get procyanidin and Radix Ginseng extract is crossed 80 mesh sieves; Merge two medicines, add lactose and magnesium stearate is an amount of, mix homogeneously; Adorn 1000 capsules, quality inspection, packing.
Embodiment 9 Cortex Pini 300g Radix Panacis Quinquefolii 150g get 75% alcohol reflux three times that Cortex Pini adds 6 times of amounts, each 2 hours, merging filtrate; Reclaim ethanol, concentrated filtrate is to the thick paste shape, and 5 times of water gagings were placed 24 hours, filtering and concentrating, and 4 times of amount 85% ethanol were placed 24 hours, filtered, and drying under reduced pressure is pulverized extractum, crosses 80 mesh sieves; Get 75% alcohol reflux three times of participating in 8 times of amounts in the West, each 2 hours, merging filtrate; Reclaim ethanol, concentrated filtrate is to thick paste shape, drying under reduced pressure; Pulverize extractum, cross 80 mesh sieves; Merge two medicines, add lactose and magnesium stearate is an amount of, mix homogeneously; Adorn 1000 capsules, quality inspection, packing.
Embodiment 10 Cortex Pini 750g Radix Panacis Quinquefolii 150g get 75% alcohol reflux three times that Cortex Pini adds 6 times of amounts, each 2 hours, merging filtrate; Reclaim ethanol, concentrated filtrate is to the thick paste shape, and 5 times of water gagings were placed 24 hours, filtering and concentrating, and 4 times of amount 85% ethanol were placed 24 hours, filtered, and drying under reduced pressure is pulverized extractum, crosses 80 mesh sieves; Get 75% alcohol reflux three times of participating in 8 times of amounts in the West, each 2 hours, merging filtrate; Reclaim ethanol, concentrated filtrate is to thick paste shape, drying under reduced pressure; Pulverize extractum, cross 80 mesh sieves; Merge two medicines, add lactose and magnesium stearate is an amount of, mix homogeneously; Adorn 1000 capsules, quality inspection, packing.
Embodiment 11 procyanidin 75g Radix Panacis Quinquefolii 150g lactose 75g magnesium stearate 1.5g get procyanidin and cross 80 mesh sieves, Radix Ginseng is pulverized, cross 80 mesh sieves, merge two medicines, add lactose 75g and magnesium stearate 1.5g, mix homogeneously; Adorn 1000 capsules, quality inspection, packing.
Embodiment 12 procyanidin 75g Radix Ginseng 450g lactose 75g magnesium stearate 1.5g get procyanidin and cross 80 mesh sieves, Radix Ginseng is pulverized, cross 80 mesh sieves, merge two medicines, add lactose 75g and magnesium stearate 1.5g, mix homogeneously; Adorn 1000 capsules, quality inspection, packing.
Embodiment 13 procyanidin 75g Radix Panacis Quinquefolii 150g lactose 75g magnesium stearate 1.5g get procyanidin and cross 80 mesh sieves; Get 75% alcohol reflux three times of participating in 8 times of amounts in the West, each 2 hours, merging filtrate reclaimed ethanol, and concentrated filtrate is to the thick paste shape, and drying under reduced pressure is pulverized extractum, crosses 80 mesh sieves; Merge procyanidin and Radix Panacis Quinquefolii extract, add lactose 75g and magnesium stearate 1.5g, mix homogeneously; Adorn 1000 capsules, quality inspection, packing.
Embodiment 14 procyanidin 75g Radix Ginseng 450g lactose 75g magnesium stearate 1.5g get procyanidin and cross 80 mesh sieves; Get 75% alcohol reflux three times that the people participates in 8 times of amounts, each 2 hours, merging filtrate reclaimed ethanol, and concentrated filtrate is to the thick paste shape, and drying under reduced pressure is pulverized extractum, crosses 80 mesh sieves; Merge procyanidin and Radix Ginseng extract, add lactose 75g and magnesium stearate 1.5g, mix homogeneously; Adorn 1000 capsules, quality inspection, packing.
Claims (10)
1. one kind has the anti-width of cloth and penetrates Chinese medicine medicine or health product with the effect of mediator's body immunity function, it is characterized in that, its active ingredient is made by procyanidin and Radix Panacis Quinquefolii or Radix Ginseng, the weight ratio of procyanidin and Radix Panacis Quinquefolii or Radix Ginseng is 1: 0.01-100, this medicine or health product also can contain physiologically acceptable carrier.
2. the medicine of claim 1 or health product, the Radix Panacis Quinquefolii wherein or the amount of Radix Ginseng can be the amounts of their extract, also can be the amounts of crude drug raw material, the weight ratio of procyanidin and Radix Panacis Quinquefolii or Radix Ginseng is 1: 0.1-10.
3. the medicine of claim 2 or health product, wherein the extract of Radix Panacis Quinquefolii or Radix Ginseng through alcohol extraction, concentrate, drying steps makes, the crude drug raw material is Radix Panacis Quinquefolii or Radix Ginseng drying, pulverizes and make, procyanidin wherein by Semen Vitis viniferae or Cortex Pini through alcohol extraction, concentrate, drying makes.
4. any medicine or the health product of claim 1-3, wherein the amount of procyanidin is the 1-2 weight portion, the amount of Radix Panacis Quinquefolii or Radix Ginseng is the 2-6 weight portion.
5. any medicine or the health product of claim 1-4, it is characterized in that described medicine or health product are tablet, capsule, oral liquid, suck agent, granule, electuary, pill, powder, suspensoid, powder, solution, injection, suppository, ointment, plaster, cream, spray, drop, patch.
6. the medicine of claim 5 or health product are capsules.
7. the medicine of claim 3 or health product is characterized in that, wherein the part by weight of effective ingredient is calculated as by crude drug, Semen Vitis viniferae or Cortex Pini: Radix Ginseng or Radix Panacis Quinquefolii=5-20: 1.
8. any medicine of claim 1-7 or the preparation method of health product is characterized in that, with procyanidin and Radix Panacis Quinquefolii or Radix Ginseng and the mixing of medicine acceptable carrier.
9. any medicine of claim 1-7 or health product are penetrated application in the medicine with the effect of mediator's body immunity function at a kind of anti-width of cloth of preparation.
10. the medicine of claim 6 or health product, wherein every capsules contains procyanidin 50-100mg, Radix Panacis Quinquefolii 100-200mg.
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| CN 02131251 CN1401365A (en) | 2002-09-19 | 2002-09-19 | Chinese health medicine |
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| Application Number | Priority Date | Filing Date | Title |
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| CN 02131251 CN1401365A (en) | 2002-09-19 | 2002-09-19 | Chinese health medicine |
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| CN1401365A true CN1401365A (en) | 2003-03-12 |
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| CN 02131251 Pending CN1401365A (en) | 2002-09-19 | 2002-09-19 | Chinese health medicine |
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| CN (1) | CN1401365A (en) |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102198195A (en) * | 2011-05-25 | 2011-09-28 | 遵义陆圣康源科技开发有限责任公司 | Antioxidative medicinal composition |
| CN102258195A (en) * | 2011-08-15 | 2011-11-30 | 通化腾龙生物科技有限公司 | Amur grape seed oligomeric proantho cyanidins (OPC)-ginseng health-care product |
| CN102631482A (en) * | 2012-05-12 | 2012-08-15 | 赵全成 | Chinese medicinal composition for preventing and treating diabetes and complications |
| CN102657334A (en) * | 2012-05-14 | 2012-09-12 | 青岛海隆达生物科技有限公司 | Hard capsules taking grape seed extracts as raw materials and preparation method for hard capsules |
| CN102669657A (en) * | 2012-04-20 | 2012-09-19 | 苏州爱斯欧蒂生物科技有限公司 | Immunity-improving health product |
| CN102716272A (en) * | 2012-06-27 | 2012-10-10 | 乔树宏 | Traditional Chinese medicine composition for senescence delaying and antifatigue as well as soft capsule and preparation method of traditional Chinese medicine composition |
| CN106692505A (en) * | 2017-01-23 | 2017-05-24 | 徐斌 | Health-care food composition and preparation method thereof |
| CN109965286A (en) * | 2019-04-16 | 2019-07-05 | 湖南泽迈生物科技有限公司 | A kind of radix polygonati officinalis grape health product composition and preparation method thereof |
| CN111149957A (en) * | 2020-01-08 | 2020-05-15 | 孟凡云 | Beverage suitable for diabetic patients and preparation method thereof |
-
2002
- 2002-09-19 CN CN 02131251 patent/CN1401365A/en active Pending
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102198195A (en) * | 2011-05-25 | 2011-09-28 | 遵义陆圣康源科技开发有限责任公司 | Antioxidative medicinal composition |
| CN102258195A (en) * | 2011-08-15 | 2011-11-30 | 通化腾龙生物科技有限公司 | Amur grape seed oligomeric proantho cyanidins (OPC)-ginseng health-care product |
| CN102669657A (en) * | 2012-04-20 | 2012-09-19 | 苏州爱斯欧蒂生物科技有限公司 | Immunity-improving health product |
| CN102631482A (en) * | 2012-05-12 | 2012-08-15 | 赵全成 | Chinese medicinal composition for preventing and treating diabetes and complications |
| CN102631482B (en) * | 2012-05-12 | 2014-01-08 | 赵全成 | Chinese medicinal composition for preventing and treating diabetes and complications |
| CN102657334A (en) * | 2012-05-14 | 2012-09-12 | 青岛海隆达生物科技有限公司 | Hard capsules taking grape seed extracts as raw materials and preparation method for hard capsules |
| CN102716272A (en) * | 2012-06-27 | 2012-10-10 | 乔树宏 | Traditional Chinese medicine composition for senescence delaying and antifatigue as well as soft capsule and preparation method of traditional Chinese medicine composition |
| CN106692505A (en) * | 2017-01-23 | 2017-05-24 | 徐斌 | Health-care food composition and preparation method thereof |
| CN109965286A (en) * | 2019-04-16 | 2019-07-05 | 湖南泽迈生物科技有限公司 | A kind of radix polygonati officinalis grape health product composition and preparation method thereof |
| CN111149957A (en) * | 2020-01-08 | 2020-05-15 | 孟凡云 | Beverage suitable for diabetic patients and preparation method thereof |
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