CN1585897A - 阳离子表面活性剂定量法 - Google Patents
阳离子表面活性剂定量法 Download PDFInfo
- Publication number
- CN1585897A CN1585897A CN02822673.9A CN02822673A CN1585897A CN 1585897 A CN1585897 A CN 1585897A CN 02822673 A CN02822673 A CN 02822673A CN 1585897 A CN1585897 A CN 1585897A
- Authority
- CN
- China
- Prior art keywords
- plate
- cationic surfactant
- sample solution
- liquid chromatography
- performance liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000003093 cationic surfactant Substances 0.000 title claims abstract description 48
- 238000000034 method Methods 0.000 title abstract description 15
- 238000004128 high performance liquid chromatography Methods 0.000 claims abstract description 27
- 239000012488 sample solution Substances 0.000 claims abstract description 22
- 239000002245 particle Substances 0.000 claims abstract description 17
- 239000003957 anion exchange resin Substances 0.000 claims abstract description 16
- 229920001577 copolymer Polymers 0.000 claims abstract description 11
- 238000002414 normal-phase solid-phase extraction Methods 0.000 claims description 19
- 239000003945 anionic surfactant Substances 0.000 claims description 15
- 238000013459 approach Methods 0.000 claims description 10
- JSAIENUMNDAGTD-UHFFFAOYSA-N benzene ethene styrene Chemical compound C1=CC=CC=C1.C=C.C=C.C=CC1=CC=CC=C1 JSAIENUMNDAGTD-UHFFFAOYSA-N 0.000 claims description 10
- 239000000523 sample Substances 0.000 claims description 10
- 239000000243 solution Substances 0.000 claims description 5
- 239000007864 aqueous solution Substances 0.000 claims description 4
- 238000012360 testing method Methods 0.000 claims description 4
- 238000002798 spectrophotometry method Methods 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 21
- 238000000926 separation method Methods 0.000 abstract description 5
- 229910001410 inorganic ion Inorganic materials 0.000 abstract description 4
- 238000011002 quantification Methods 0.000 abstract description 2
- RLGQACBPNDBWTB-UHFFFAOYSA-N cetyltrimethylammonium ion Chemical compound CCCCCCCCCCCCCCCC[N+](C)(C)C RLGQACBPNDBWTB-UHFFFAOYSA-N 0.000 description 7
- 230000007613 environmental effect Effects 0.000 description 5
- 239000007790 solid phase Substances 0.000 description 5
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 4
- 238000011084 recovery Methods 0.000 description 4
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 3
- 239000004372 Polyvinyl alcohol Substances 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 229920002451 polyvinyl alcohol Polymers 0.000 description 3
- HNSDLXPSAYFUHK-UHFFFAOYSA-N 1,4-bis(2-ethylhexyl) sulfosuccinate Chemical compound CCCCC(CC)COC(=O)CC(S(O)(=O)=O)C(=O)OCC(CC)CCCC HNSDLXPSAYFUHK-UHFFFAOYSA-N 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 230000008033 biological extinction Effects 0.000 description 2
- 239000012141 concentrate Substances 0.000 description 2
- -1 decyl dimethyl benzyl ammonium ions Chemical class 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 229920002521 macromolecule Polymers 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000004445 quantitative analysis Methods 0.000 description 2
- 239000011347 resin Substances 0.000 description 2
- 229920005989 resin Polymers 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 238000004513 sizing Methods 0.000 description 2
- 239000004809 Teflon Substances 0.000 description 1
- 229920006362 Teflon® Polymers 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000003011 anion exchange membrane Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 229910052731 fluorine Inorganic materials 0.000 description 1
- 239000011737 fluorine Substances 0.000 description 1
- 125000001153 fluoro group Chemical group F* 0.000 description 1
- 230000009931 harmful effect Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-M hydroxide Chemical compound [OH-] XLYOFNOQVPJJNP-UHFFFAOYSA-M 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000006277 sulfonation reaction Methods 0.000 description 1
- 229940124597 therapeutic agent Drugs 0.000 description 1
- HFUMBCROCNBYQL-UHFFFAOYSA-N trimethyl(octadecanoyl)azanium Chemical compound CCCCCCCCCCCCCCCCCC(=O)[N+](C)(C)C HFUMBCROCNBYQL-UHFFFAOYSA-N 0.000 description 1
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/22—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
- B01J20/26—Synthetic macromolecular compounds
- B01J20/261—Synthetic macromolecular compounds obtained by reactions only involving carbon to carbon unsaturated bonds
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/28—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
- B01J20/28014—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their form
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/96—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation using ion-exchange
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/18—Water
- G01N33/1813—Specific cations in water, e.g. heavy metals
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
- B01D15/361—Ion-exchange
- B01D15/363—Anion-exchange
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
- G01N30/14—Preparation by elimination of some components
- G01N2030/143—Preparation by elimination of some components selective absorption
Landscapes
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Pathology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
样品溶液中阳离子表面活性剂的定量方法，其特征在于，将样品溶液通过由苯乙烯－二乙烯基苯共聚物颗粒填充的板与用阴离子交换树脂填充的板，然后进行高效液相色谱。利用本发明的方法可以对与无机离子和高浓度AS共存的河流水和湖沼水中ppb级或更低水平的CS进行分离定量。
Description
技术领域
本发明涉及能在环境水等的阴离子表面活性剂和阳离子表面活性剂共存的水溶液中，准确且迅速地对阳离子表面活性剂进行定量的方法及其相关设备。
背景技术
阳离子表面活性剂(CS)作为护发素、治疗剂(treatment)、衣料柔软润饰剂等的主要成分而被广泛应用。但是，由于CS具有杀菌消毒性、难于被微生物降解、其对水生生物的不良影响为人们所忧虑。因此，对环境水中的CS进行定量非常重要，需要建立低浓度CS的快速分析法。
本发明的发明人现利用导电率检测高效液相色谱(HPLC)，以建立环境水中CS的高灵敏性分离定量法为目的，对亲水性高分子凝胶柱作为分离柱的HPLC***进行了研究。由所得的结果构建出与现有的利用导电率和间接UV吸光检测的HPLC法相比，测定范围低出一位以上(0.03-0.04μM)的高灵敏的HPLC***。
由于河流中的CS浓度在ppb水平或以下，待进行定量的样品中需要进行CS的浓缩以及无机离子和阴离子表面活性剂(AS)之间的分离。
因此，本发明的目的是提供一种对含有AS和CS的环境水等的水溶液中的仅对CS进行精确定量的方法。
发明内容
因此，本发明的发明人为将样品溶液中的AS和CS分开，安装了用各种树脂填充的板进行各种检测，完全出乎意料地发现用苯乙烯-二乙烯基苯共聚物颗粒填充的板与用阴离子交换树脂填充的板进行组合，样品溶液通过这两个板后，AS被选择性地去除，CS得以浓缩，随后必要时进行固相提取后进行HPLC，可以对低浓度的CS进行正确的定量，由此完成了本发明。
也就是说，本发明提供一种样品溶液中阳离子表面活性剂的定量方法，该方法的特征在于：将样品溶液通过由苯乙烯-二乙烯基苯共聚物颗粒填充的板与用阴离子交换树脂填充的板，然后进行高效液相色谱。
其次，本发明提供一种样品溶液中阳离子表面活性剂的定量方法，该方法的特征在于：将样品溶液通过由苯乙烯-二乙烯基苯共聚物颗粒填充的板与用阴离子交换树脂填充的板，再通过固相提取柱，然后进行高效液相色谱。
再次，本发明还提供一种用于样品溶液中阳离子表面活性剂的定量方法的设备，该设备由用苯乙烯-二乙烯基苯共聚物颗粒填充的板与用阴离子交换树脂填充的板与高效液相色谱设备组成。
本发明还进一步提供一种用于样品溶液中阳离子表面活性剂的定量方法的设备，该设备由用苯乙烯-二乙烯基苯共聚物颗粒填充的板与用阴离子交换树脂填充的板与固相提取柱，以及高效液相色谱设备组成。
附图说明
图1是用于本发明的在线固相提取/HPLC***的示意图。
图2是用河流中的水作为样品，经过SDB-XD(SDB颗粒填充板)和ANION-SR(阴离子交换树脂填充板)的板预处理-在线固相提取-HPLC分离后结果的示意图。
图3用河流中的水作为样品，经过SDB-XD和ANION-SR板预处理-在线固相提取-HPLC对CS进行定量分离中，CTMA(十六烷基三甲基铵离子)和TMSA(三甲基硬脂酰铵离子)的标准添加量实验结果。
图中的符号如下所示。
1：样品溶液 7：洗提液
2：纯水 8：废弃物
3：泵 9：分离柱
4：固相提取柱 10：抑制器
5：注射器 11：检测器
6：泵 12：再生液
具体实施方式
本发明的定量法所用的样品溶液可以是任何含有CS的溶液，更具体地可列举出的有河流水、湖沼水等环境水。在河流水和湖沼水中，除CS以外多数情况下还含有AS、无机离子，对它们进行CS定量此前没有很好的方法。
在本发明中，样品溶液首先通过用苯乙烯-二乙烯基苯共聚物(SDB)颗粒填充的板与用阴离子交换树脂填充的板，经过上述的两板可去除样品溶液中的AS、将CS选择性地回收。在许多种的板中，仅这两种板的组合可去除AS，且CS良好的回收率是完全出乎意料的。所述板中的任何一个都可以用纤维填充颗粒固定，所用的纤维是含氟树脂，优选例如特氟隆(注册商标)等。另外，作为阴离子交换树脂，优选季铵盐型阴离子交换树脂。所述的板可以是由スリ一エム公司获得的商品名为エムポア板SDB-XD、エムポア板ANION-SR。而且所述的板任何一个在先使用均可以。
然后通过上述板后的样品，在必要时需经固相提取柱后，再进行高效液相色谱(HPLC)。经过固相提取可将样品中的CS高度浓缩、由此可使通过HPLC进行CS定量的灵敏性大大提高。固相提取优选使用在线固相提取进行浓缩。
如图1所示，通过泵3将纯水2和样品溶液1供给固相提取柱4后，按一定的循环次数进行在线固相提取。用作固相提取中的固相是填充了亲水高分子的固相，例如可使用高度交联的聚乙烯醇凝胶所填充的固相。所述的聚乙烯醇凝胶可以是例如，Hhodex AsahipakGF-310HP(昭和电工(株式会社))。
将经CS浓缩的样品和通过了上述板的样品进行HPLC。HPLC是由图1中的分离柱9、注射器10和检测器11组成。其中，作为分离柱的亲水性高分子填充柱，例如高度交联的聚乙烯醇凝胶填充的柱，更具体地优选Shodex Asahipak GF-310HP(昭和电工(株式会社))。作为注射器可使用阴离子交换膜，例如DIONEX CMMS-II(日本ダイオネクス(株式会社))。
作为检测器11，可列举出的有：导电率检测器、间接UV吸光检测器、质量分析检测器，特别优选导电率检测器和质量分析检测器。应用质量分析检测时，HPLC之前即使不进行固相提取也可以对很低的浓度进行测定，应用导电率测定时，优选在进行HPLC之前进行固相提取。
应用本发明的方法，以含有超过CS100倍以上的AS的河流或湖沼水为对象进行检测时，可以对低于ppb水平的CS进行定量。
实施例
通过下述的实施例可对本发明进行更具体地说明，但本发明不受这些实施例的任何限制。
实施例1
(1)图1是用作在线固相提取/HPLC***的示意图。4，4-联吡啶/盐酸/乙腈用作洗提液7，Shodex Asahipak GF-310HQ(4.6mmI.D.×150mm，6μm)用作分离柱9。进行固相提取的柱4使用填充了与分离柱相同材质但粒径不同的填充剂的柱(4.6mmI.D.×10mm，9μm)，注射器10使用DIONEX CMMS-II(日本ダイオネクス(株式会社))，四丁基铵氢氧化物溶液用作再生液12，样品1可用十六烷基三甲基铵离子(CTMA)、四癸基二甲基苄基铵离子(TDDBA)和三甲基硬脂酰铵离子(TMSA)三种。此外使用エムポア板SDB-XD(SDB颗粒填充板)、CATION-SR(阳离子交换树脂填充板)、ANION-SR(阴离子交换树脂填充板)、SDB-RPS(磺化SDB颗粒填充板)。
(2)首先以50mM浓度的CS用エムポア板对CS的吸收浓缩进行研究。即，在样品溶液通过所述板后，用图1中的在线固相提取/HPLC对CS进行定量。向样品溶液中添加盐酸使溶液达到0.1M，以防止CS吸附到玻璃器皿的壁上。结果对CS的吸收效率的优劣顺序为SDB-RPS＞SDB-XD＞ANION-SR。但是，向溶液中添加5μM左右的AS则可以观察到每板中的CS回收率均明显下降。在各种检测结果中，将SDB颗粒填充的板SDB-XD和阴离子交换树脂填充的板ANION-SR配套使用，在将AS去除的同时可以得到CS的高回收率(95％以上)。这种高回收率是其他板的组合形式所不能达到的。而且，SDB-XD和ANION-SR以顺序或逆序配套使用所得的结果相同。
(3)根据(2)中的结果，将本发明的方法用于测定实际的河流水。图2是从流经船桥市的海老河采集的河流水的色谱图。由于共存物对CS保存时间变化的影响，重复测定的结果确定了CTMA和TMSA的存在。而且经过质谱分析也确认了CTMA和TMSA的存在。据此，用这些与CS相关的标准添加法进行定量，如图3所示获得了良好的直线关系。根据所得结果，计算出CTMA和TMSA在各河流水中的浓度为1.7nM(0.54ppb)、7.0nM(2.5ppb)。
工业实用性
利用本发明的方法可以对与无机离子和高浓度AS共存的河流水和湖沼水中ppb级或更低水平的CS进行分离定量。
Claims (8)
1、一种对样品溶液中阳离子表面活性剂的定量方法，其特征在于，将样品溶液通过由苯乙烯-二乙烯基苯共聚物颗粒填充的板与用阴离子交换树脂填充的板，然后进行高效液相色谱。
2、如权利要求1所述的定量方法，其特征在于，所述样品是含有阳离子表面活性剂和阴离子表面活性剂的水溶液。
3、如权利要求1或2所述的定量方法，其特征在于，所述高效液相色谱的测定手段是质谱分析。
4、一种样品溶液中阳离子表面活性剂的定量方法，其特征在于，溶液通过由苯乙烯-二乙烯基苯共聚物颗粒填充的板与用阴离子交换树脂填充的板，再通过固相提取柱，然后进行高效液相色谱。
5、如权利要求4所述的定量方法，其特征在于，所述样品是含有阳离子表面活性剂和阴离子表面活性剂的水溶液。
6、如权利要求4或5所述的定量方法，其特征在于：所述高效液相色谱的测定手段是导电率和质谱分析。
7、一种用于检测样品溶液中阳离子表面活性剂的装置，其特征在于，该设备由用苯乙烯-二乙烯基苯共聚物颗粒填充的板、用阴离子交换树脂填充的板以及高效液相色谱设备组成。
8、一种用于检测样品溶液中阳离子表面活性剂的装置，其特征在于，该设备由用苯乙烯-二乙烯基苯共聚物颗粒填充的板、用阴离子交换树脂填充的板、固相提取柱以及高效液相色谱设备组成。
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2001356859A JP2003156483A (ja) | 2001-11-22 | 2001-11-22 | 陽イオン界面活性剤の定量法 |
| JP356859/2001 | 2001-11-22 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1585897A true CN1585897A (zh) | 2005-02-23 |
Family
ID=19168302
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN02822673.9A Pending CN1585897A (zh) | 2001-11-22 | 2002-11-15 | 阳离子表面活性剂定量法 |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US20050010002A1 (zh) |
| EP (1) | EP1447662A1 (zh) |
| JP (1) | JP2003156483A (zh) |
| CN (1) | CN1585897A (zh) |
| AU (1) | AU2002349681A1 (zh) |
| WO (1) | WO2003044518A1 (zh) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102085490A (zh) * | 2010-12-27 | 2011-06-08 | 天津博纳艾杰尔科技有限公司 | 一种固相萃取混合填料及固相萃取柱 |
| CN101395476B (zh) * | 2005-12-14 | 2012-10-03 | 电化生研株式会社 | 多重耐药葡萄球菌的免疫色谱检测方法和诊断试剂盒 |
| CN104792938A (zh) * | 2015-04-10 | 2015-07-22 | 中国石油大学(华东) | 一种测定co2乳液在渗流过程中表面活性剂浓度分布的装置及方法 |
| CN109342638A (zh) * | 2018-11-29 | 2019-02-15 | 浙江树人学院 | 一种利用阳离子交换抑制电导法检测卡内腈、季铵盐及其杂质铵、钾、钙离子含量的方法 |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2316035A2 (en) * | 2008-07-16 | 2011-05-04 | Radiometer Medical ApS | Thrombin substrate and assay for determining the level of bioactive thrombin in a sample |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH01140061A (ja) * | 1987-11-26 | 1989-06-01 | Shimadzu Corp | 高速液体クロマトグラフィによるエーテル系界面活性剤の分析方法 |
| JPH05196606A (ja) * | 1991-09-03 | 1993-08-06 | Kao Corp | 界面活性剤の微量定量分析方法 |
| JP3345266B2 (ja) * | 1996-05-28 | 2002-11-18 | 横河電機株式会社 | 陰イオン界面活性剤測定装置 |
| JPH1073578A (ja) * | 1996-08-30 | 1998-03-17 | Lion Corp | 界面活性剤を構成する親油基成分の分離方法及び測定方法 |
-
2001
- 2001-11-22 JP JP2001356859A patent/JP2003156483A/ja active Pending
-
2002
- 2002-11-15 AU AU2002349681A patent/AU2002349681A1/en not_active Abandoned
- 2002-11-15 US US10/495,575 patent/US20050010002A1/en not_active Abandoned
- 2002-11-15 EP EP20020781789 patent/EP1447662A1/en not_active Withdrawn
- 2002-11-15 WO PCT/JP2002/011937 patent/WO2003044518A1/ja not_active Application Discontinuation
- 2002-11-15 CN CN02822673.9A patent/CN1585897A/zh active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101395476B (zh) * | 2005-12-14 | 2012-10-03 | 电化生研株式会社 | 多重耐药葡萄球菌的免疫色谱检测方法和诊断试剂盒 |
| CN102085490A (zh) * | 2010-12-27 | 2011-06-08 | 天津博纳艾杰尔科技有限公司 | 一种固相萃取混合填料及固相萃取柱 |
| CN104792938A (zh) * | 2015-04-10 | 2015-07-22 | 中国石油大学(华东) | 一种测定co2乳液在渗流过程中表面活性剂浓度分布的装置及方法 |
| CN104792938B (zh) * | 2015-04-10 | 2016-08-24 | 中国石油大学(华东) | 一种测定co2乳液在渗流过程中表面活性剂浓度分布的装置及方法 |
| CN109342638A (zh) * | 2018-11-29 | 2019-02-15 | 浙江树人学院 | 一种利用阳离子交换抑制电导法检测卡内腈、季铵盐及其杂质铵、钾、钙离子含量的方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| AU2002349681A1 (en) | 2003-06-10 |
| US20050010002A1 (en) | 2005-01-13 |
| EP1447662A1 (en) | 2004-08-18 |
| WO2003044518A1 (fr) | 2003-05-30 |
| JP2003156483A (ja) | 2003-05-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Garcia-Ac et al. | On-line solid-phase extraction of large-volume injections coupled to liquid chromatography-tandem mass spectrometry for the quantitation and confirmation of 14 selected trace organic contaminants in drinking and surface water | |
| Alexiadou et al. | Molecularly imprinted polymers for bisphenol A for HPLC and SPE from water and milk | |
| Gros et al. | Trace level determination of β-blockers in waste waters by highly selective molecularly imprinted polymers extraction followed by liquid chromatography–quadrupole-linear ion trap mass spectrometry | |
| Venn et al. | Synthesis and properties of molecular imprints of darifenacin: the potential of molecular imprinting for bioanalysis | |
| Du et al. | An in situ immobilized pipette tip solid phase microextraction method based on molecularly imprinted polymer monolith for the selective determination of difenoconazole in tap water and grape juice | |
| Hoshina et al. | Simultaneous determination of non-steroidal anti-inflammatory drugs in river water samples by liquid chromatography–tandem mass spectrometry using molecularly imprinted polymers as a pretreatment column | |
| Fu et al. | Preparation and evaluation of molecularly imprinted polymers as selective SPE sorbents for the determination of cathinones in river water | |
| CN1585897A (zh) | 阳离子表面活性剂定量法 | |
| Ahmadi et al. | Electrochemically controlled in-tube solid phase microextraction of naproxen from urine samples using an experimental design | |
| Liu et al. | Determination of trace macrolide antibiotics in milk with online solid‐phase extraction with an ionic‐liquid‐based monolithic column | |
| Shao et al. | On-line enrichment of N-glycans by immobilized metal-affinity monolith for capillary electrophoresis analysis | |
| CN106526062A (zh) | 一种测定三乙基铝中痕量氯离子的离子方法 | |
| Gillespie et al. | Using scanning contactless conductivity to optimise photografting procedures and capacity in the production of polymer ion-exchange monoliths | |
| Sedldk | Structure and dynamics of polyelectrolyte solutions by light scattering | |
| Ning et al. | Preparation of monodisperse agglomerated pellicular anion-exchange resins compatible with high-performance liquid chromatography solvents for ion chromatography | |
| Mikes et al. | Ion-exchange derivatives of spheron: III. Carboxylic cation exchangers | |
| Kok et al. | Peer Reviewed: Electrokinetic Separations for Synthetic Polymers. | |
| JPS6031055A (ja) | 試料の前処理法 | |
| Goto et al. | Separation of electrolyte and nonelectrolyte by an ion retardation resin | |
| Li et al. | Preparation of a porous functional polymer and its application in the separation of small molecules in conjunction with HPLC | |
| De Borba et al. | Determination of inorganic anions in environmental waters using a hydroxide-selective column | |
| CN1226622C (zh) | 氢氧根离子的分离方法和分析方法及离子色谱装置 | |
| Ahmed et al. | Determination of chlordiazepoxide in pure drug samples, pharmaceutical dosage forms and environmental wastewater samples using high performance liquid chromatographic method | |
| Xu et al. | Fluoride Determination by Ion Chromatography in Fluorocarbon Coatings | |
| Mitsumori et al. | Quantitative analysis of cyclodextrins by electrochemical detector and column with core-shell type filler |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |