CN1563406A - 重组幽门螺杆菌热休克蛋白a基因工程疫苗制备中的纯化工艺 - Google Patents
重组幽门螺杆菌热休克蛋白a基因工程疫苗制备中的纯化工艺 Download PDFInfo
- Publication number
- CN1563406A CN1563406A CN 200410022360 CN200410022360A CN1563406A CN 1563406 A CN1563406 A CN 1563406A CN 200410022360 CN200410022360 CN 200410022360 CN 200410022360 A CN200410022360 A CN 200410022360A CN 1563406 A CN1563406 A CN 1563406A
- Authority
- CN
- China
- Prior art keywords
- purifying
- purification
- nickel ion
- renaturation
- superdex
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
Description
Claims (8)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN 200410022360 CN1241938C (zh) | 2004-04-20 | 2004-04-20 | 重组幽门螺杆菌热休克蛋白a基因工程疫苗制备中的纯化方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN 200410022360 CN1241938C (zh) | 2004-04-20 | 2004-04-20 | 重组幽门螺杆菌热休克蛋白a基因工程疫苗制备中的纯化方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1563406A true CN1563406A (zh) | 2005-01-12 |
CN1241938C CN1241938C (zh) | 2006-02-15 |
Family
ID=34480059
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN 200410022360 Expired - Fee Related CN1241938C (zh) | 2004-04-20 | 2004-04-20 | 重组幽门螺杆菌热休克蛋白a基因工程疫苗制备中的纯化方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN1241938C (zh) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104962541A (zh) * | 2015-03-31 | 2015-10-07 | 芜湖康卫生物科技有限公司 | 口服重组幽门螺杆菌疫苗的纯化工艺 |
CN107417765A (zh) * | 2017-09-26 | 2017-12-01 | 珠海联邦制药股份有限公司 | 大肠杆菌自溶表达体系中分离纯化重组蛋白的方法 |
CN107459575A (zh) * | 2016-06-03 | 2017-12-12 | 硕英生医股份有限公司 | 具有抑制病原体的免疫抑制功能的单克隆抗体,其抗原结合片段,以及产生该抗体的杂交瘤 |
CN112812969A (zh) * | 2021-01-12 | 2021-05-18 | 福建基诺厚普生物科技有限公司 | 一种基因工程重组表达多肽的***纯化方法 |
-
2004
- 2004-04-20 CN CN 200410022360 patent/CN1241938C/zh not_active Expired - Fee Related
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104962541A (zh) * | 2015-03-31 | 2015-10-07 | 芜湖康卫生物科技有限公司 | 口服重组幽门螺杆菌疫苗的纯化工艺 |
CN107459575A (zh) * | 2016-06-03 | 2017-12-12 | 硕英生医股份有限公司 | 具有抑制病原体的免疫抑制功能的单克隆抗体,其抗原结合片段,以及产生该抗体的杂交瘤 |
CN107459575B (zh) * | 2016-06-03 | 2021-08-31 | 硕英生医股份有限公司 | 具有抑制病原体的免疫抑制功能的单克隆抗体,其抗原结合片段,以及产生该抗体的杂交瘤 |
CN107417765A (zh) * | 2017-09-26 | 2017-12-01 | 珠海联邦制药股份有限公司 | 大肠杆菌自溶表达体系中分离纯化重组蛋白的方法 |
CN107417765B (zh) * | 2017-09-26 | 2020-12-04 | 珠海联邦制药股份有限公司 | 大肠杆菌自溶表达体系中分离纯化重组蛋白的方法 |
CN112812969A (zh) * | 2021-01-12 | 2021-05-18 | 福建基诺厚普生物科技有限公司 | 一种基因工程重组表达多肽的***纯化方法 |
Also Published As
Publication number | Publication date |
---|---|
CN1241938C (zh) | 2006-02-15 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Klemm et al. | F7 and type 1-like fimbriae from three Escherichia coli strains isolated from urinary tract infections: protein chemical and immunological aspects | |
CN106967696B (zh) | 一种蒿属植物花粉的过敏原及其应用 | |
CN1189186A (zh) | 流感嗜血杆菌的omp26抗原 | |
WO2013065009A1 (en) | A sortase-click reaction suite for synthesis of multivalent dendrimeric protein assembly | |
CN1241938C (zh) | 重组幽门螺杆菌热休克蛋白a基因工程疫苗制备中的纯化方法 | |
CN110642927B (zh) | 一种蛋白在制备预防化脓隐秘杆菌感染的药物中的应用 | |
US7517533B2 (en) | Pseudomonas aeruginosa antigens | |
CN1844398A (zh) | 一种金属硫蛋白融合表达方法及其应用 | |
CN108611327A (zh) | 一种猪圆环病毒2型病毒样颗粒的分离纯化方法 | |
CN1275981C (zh) | 人血清白蛋白多聚体的单体化方法 | |
JPH06511154A (ja) | 組換えBorreliaタンパクの製造法 | |
CN109337853B (zh) | 一种铜绿假单胞菌基因工程疫苗候选抗原rExoU的纯化方法 | |
CN108251445B (zh) | 一种gcrv ii s9及s10重组蛋白规模化的制备工艺及应用 | |
CN112999341B (zh) | 迟缓爱德华氏菌外膜蛋白疫苗及其制备和应用 | |
CA2622635A1 (en) | Polynucleotides of haemophilus parasuis and its use | |
JPH10506521A (ja) | ヘリコバクターピロリニッケル結合タンパク質 | |
CN113754782A (zh) | 一种幽门螺旋杆菌卵黄抗体及其制备方法和应用 | |
Young et al. | Reassembly of a fimbrial hemagglutinin from Pseudomonas solanacearum after purification of the subunit by preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis | |
RU2799574C1 (ru) | Способ получения холерного токсина для контроля производства холерной химической вакцины | |
JP3823268B2 (ja) | ヘリコバクター・ピロリの細胞死誘導剤 | |
CN116217680B (zh) | 碱稳定性高的免疫球蛋白结合蛋白及其应用 | |
CN113896775A (zh) | 幽门螺杆菌特异性免疫原性肽段 | |
EP2908852B1 (en) | Lyme disease vaccine | |
US20210308245A1 (en) | Construction and use of tandem epitope polypeptide of outer membrane protein of campylobacter jejuni, gene, recombinant plasmid and recombinant bacteria | |
JP3693900B2 (ja) | 1a5b構造を有する蛋白質の製造法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
ASS | Succession or assignment of patent right |
Owner name: CHONGQING KANGWEI BIOLOGICAL TECHNOLOGY CO., LTD. Free format text: FORMER OWNER: MILITARY MEDICAL UNIV NO.3, P.L.A. CLINICAL MICROBIOLOGY STAFF ROOM, MEDICAL LABORATORY DEPARTMENT, THIRD MILITARY MEDICAL UNIVERSITY Effective date: 20060630 |
|
C41 | Transfer of patent application or patent right or utility model | ||
TR01 | Transfer of patent right |
Effective date of registration: 20060630 Address after: 400038 Chongqing Shapingba city gaotanyan Garden City Yuyuan Yan building B 7-5 Patentee after: Chongqing Kangwei biotechnology Ltd. Address before: Chongqing city Shapingba street 400038 gaotanyan No. 30 Department of clinical microbiology Patentee before: Third Military Medical University, Chinese People's Liberation Army |
|
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20060215 Termination date: 20180420 |
|
CF01 | Termination of patent right due to non-payment of annual fee |