CN1560048A - Extractive of smalt and extracting process thereof - Google Patents
Extractive of smalt and extracting process thereof Download PDFInfo
- Publication number
- CN1560048A CN1560048A CNA2004100236487A CN200410023648A CN1560048A CN 1560048 A CN1560048 A CN 1560048A CN A2004100236487 A CNA2004100236487 A CN A2004100236487A CN 200410023648 A CN200410023648 A CN 200410023648A CN 1560048 A CN1560048 A CN 1560048A
- Authority
- CN
- China
- Prior art keywords
- extract
- red
- halophytes
- cyanidin
- floral leaf
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention is an anthocyanin extract and its extraction method, extracted from he flowers and leaves of the halophytes: Suaeda heteoptera Kitag., Salicorniaeuropaea and Suaedasalsa (L.) Pal. It is a hygroscopic purplish red powdery substance, containing 25-70% anthocyanin; its maximum absorption wavelength is 529 nm. The color of its water solution turns with the pH value from deep red->red->light red->light yellow->deep yellow; its water solution is placed for 72 hours in the natural light, the color changes from red to light red, and the light absorption value at 529 nm wavelength is reduced from 2.0 to 1.80. As Na+ and Cu2+ ions exist, the color of the water solution turns from shallow red to kelly; as Fe3+ and/or Zn2+ ions exist, the color is deep red, here the absorption wavelengths of the water solution are 560 and 570 nm. The invention extracted anthocyanin by using the flowers and leaves of the halophytes, thus enlarging the range of raw materials. And the yield of anthocyanin is high.
Description
Technical field
The present invention relates to a kind of synthetic, the pre-preventing thrombosis and effects such as arteriosclerosis, anticancer enzymic activity that can promote the human body rhodopsin; can vision protection, biological medicine---the cyanidin extract of diseases such as anti-ageing, treatment cardiovascular and cerebrovascular, cancer, arthritis, it belongs to the biological medicine technology field.
Background technology
As everyone knows, anthocyanidin is the intravital class water colo(u)r of plant.Anthocyanidin acylations and anthocyanidin plant tissue culture are most promising two fields of its suitability for industrialized production.Evidence suggests, the not only nontoxic and no mutagenesis of anthocyanidin, and the characteristic for the treatment of disease is arranged.Anthocyanidin is learned at the eye medical courses in general, treats various blood circulation disorders, and inflammatory diseases is effective in cure.Pay close attention to the anti-oxidation characteristics of anthocyanidin and associated class flavonoid substance recently, cause it working aspect minimizing coronary heart disease.In people's pay attention to day by day health, today of back to nature.Anthocyanidin also can be used for treating diabetic retinopathy, galactocele, the microcirculation disease that treatment is caused by the capillary vessel fragility, the normal saturating property of maintenance blood vessel.Also can be used for preventing the atherosclerosis of the rabbit that cholesterol causes, as tumor inhibitor, vasoprotector, radio-protector and antiinflammatory agents etc.As a kind of natural food colour---the anthocyanidin in the foodstuff additive,, be subjected to people's favor day by day because of its unique anti-oxidation characteristics and characteristic good for health.
Existing anthocyanidin mostly is by the edibility plant and really extracts purified, as bilberry anthocyanidin, vaccinium oxycoccus pigment, bilberry anthocyanosides.The method of its extraction is a lot, and refining as (1) resin method: the extraction spent ion exchange resin by grape skin color is removed carbohydrate and organic acid.(2) refining various types of ultra-filtration membranes and the reverse osmosis membrane of utilizing of membrane sepn made with extra care pigment with filtering form.Anthocyanidin also can and concentrate by ultrafiltration cellulose acetate film purifying: (3) recrystallization method.
As everyone knows, Suaeda heteroptera (Suaeda heterotera Kitagawa) and Suaeda salsa are Chenopodiaceae Suaeda plant, are annual herbs.China North China district, NORTHEAST REGION IN, the northwest, Shandong, Henan, Jiangsu, zhejiang and other places beach saline-alkali soil all has distribution on the ground.Suaeda heteroptera mainly distributes and lies in the above coastal waters beach of equal tide line, physical features is smooth, canescence salt efflorescence bare area patch is arranged more, be tangible zonal arrangement, the residing soil texture is a silty loam in antecedent soil moisture or saltiness in the location more than 1.5%, plant smear short and small, general 15-20 centimetre, branch is few, is red-purple in the 8-9 month, becomes a big wonder of strand mud bank.Alkali fluffy (Suaeda salsa) has another name called the salt wormwood artemisia, Chenopodiaceae annual herb plant.The protein content of its fresh and tender cauline leaf accounts for 40% of dry-matter, equates with soybean; Contain rich in amino acid, the bright taper branch of per 100 grams contains 1.75 milligrams of carotene; 20.10 milligrams of Wei ShengsuB2s, 78 milligrams of vitamins Cs; Also contain other trace elements, as Ca, P, Fe, Zn, Se etc., wherein many indexs all are higher than spirulina.
Summary of the invention
The objective of the invention is to enlarge the raw material range that anthocyanidin extracts further, high extraction ground extracts the extract that preparation contains anthocyanidin, to satisfy people's pay attention to day by day health, the demand of back to nature.
Task of the present invention is finished by following technical scheme, developed a kind of cyanidin extract, described extract is by extracting in the halophytes floral leaf, it has the hygroscopic red-purple powdery substance that is, the anthocyanidin that contains 25-70% in this extract, this extract is water-soluble, is slightly soluble in ethanol and trichloromethane, is insoluble to benzene, toluene, ether, sherwood oil; This extract is 529nm at the visible region maximum absorption wavelength; The color that the aqueous solution of this extract presents, and presents by dark red → red → pale red → pale yellow → Huang → deep yellow variation by 3 to 13 variation with the pH value; The aqueous solution of this extract was placed under natural light after 72 hours, and its solution appearance color becomes light red by redness, and the absorbance value under the 529nm wavelength drops to 1.80 by 2.0; And UV-light does not have obvious influence to the aqueous solution of this extract.
Described this solution of extract is also at Na
+Or Cu
2+When ion existed, its color became yellow-green colour by light red; At Fe
3+And/or Zn
2+When existing, its color presents scarlet, and this moment, the aqueous solution absorbing wavelength of this extract was 560 and 570nm.
Described halophytes is: Suaeda heteroptera (suaeda heteroptera), salicornia europaeal (Salicorniaeuropaea), Suaeda salsa (suaeda salsa).
By the step of extracting the extracting method of cyanidin extract in the halophytes floral leaf be,
A), will handle clean quantitative halophytes floral leaf, put into refiner, add 1-3 clean water doubly, homogenized 5-15 minute, gained halophytes floral leaf slurries are put into whizzer,, get supernatant liquor and carry out vacuum filtration again through centrifugal treating 10-30 minute, remove the remaining cellulosic in this supernatant liquor, promptly obtain halophytes floral leaf extracting solution;
B), get above-mentioned halophytes floral leaf extracting solution, add the polar solvent of this extracting liquid volume 30-70% and the non-polar solvent of 20-40%, mix and stirred 10-30 minute, the normal temperature standing demix extracts, the upper strata is green oil reservoir, and lower floor is red water layer, gets this water layer through concentrating under reduced pressure, spray-dried again, obtain red-purple powdery substance-cyanidin extract.
Described polar solvent, it is an acetone, or propyl alcohol, or ethanol, or ethyl acetate, wherein one or more.
Described by the extracting method that extracts cyanidin extract in the halophytes floral leaf, it is characterized in that: described non-polar solvent, it is a sherwood oil, or ether, or methylene dichloride, or chloroform, or contains the N-alkanes hydro carbons of 5-8 carbon.
By the step of extracting the another kind of extracting method of cyanidin extract in the halophytes floral leaf be,
A), will handle clean quantitative halophytes floral leaf drying and pulverize after, add 1-3 clean water doubly, under 30-50 ℃ of temperature, soak and extracted 30-60 minute, with soaked extracting solution through centrifugal, filter after, get filtrate and obtain halophytes floral leaf extracting solution;
B), above-mentioned halophytes floral leaf extracting solution is adopted AP-8 type polymeric adsorbent refinement treatment, with the ethanolic soln wash-out of 60%-80%, collect this ethanol eluate;
C), the gained ethanol eluate puts into rotatory evaporator again, evaporation concentration, again through vacuum concentration that this elutriant after concentrating is spray-dried again, promptly obtain having hygroscopic red-purple powder material-cyanidin extract.
Described AP-8 polymeric adsorbent, its dress post specification is: resin bottom face 1.5 * 25cm, resin load height 15cm.
Described vacuum concentration is under 60 ℃ of temperature, carries out under vacuum tightness-0.08Mpa.
Described spraying drying is 240 ℃ of air inlet temperatures, and 80 ℃ of air outlet temperatures carry out under the condition of air compressor machine pressure 0.4MPa.
The invention has the advantages that: because cyanidin extract of the present invention is by extracting in the halophytes floral leaf.Described halophytes is: Suaeda heteroptera (suaeda heteroptera), salicornia europaeal (Salicornia europaea), Suaeda salsa (suaeda salsa).The present invention uses above-mentioned halophytes floral leaf to extract anthocyanidin first, and this just enlarges the raw material range that anthocyanidin extracts.The high yield of cyanidin extract of the present invention makes preparation contain this extract of anthocyanidin, is enough to satisfy people's pay attention to day by day health, the demand of back to nature.Cyanidin extract of the present invention is to have the hygroscopic red-purple powdery substance that is, and contains the anthocyanidin of 25-70% in this extract.The physico-chemical property of this extract is as follows: soluble in water, be slightly soluble in ethanol and trichloromethane, and be insoluble to benzene, toluene, ether, sherwood oil; This extract is 529nm at the visible region maximum absorption wavelength; The color that the aqueous solution of this extract presents, and presents by dark red → red → pale red → pale yellow → Huang → deep yellow variation by 3 to 13 variation with the pH value; The aqueous solution of this extract was placed under natural light after 72 hours, and its solution appearance color becomes light red by redness, and the absorbance value under the 529nm wavelength drops to 1.80 by 2.0; And UV-light does not have obvious influence to the aqueous solution of this extract.These physico-chemical properties intuitively can be surveyed, and differentiate to have obvious color distinctive and separation property easily.Through experiment confirm: cyanidin extract of the present invention can promote synthetic, the pre-preventing thrombosis and the effects such as arteriosclerosis, anticancer enzymic activity of human body rhodopsin.Therefore, energy vision protection, anti-ageing, diseases such as treatment cardiovascular and cerebrovascular, cancer, arthritis.Especially the anthocyanidin that from the Suaeda heteroptera leaf is spent, extracts, its not only nontoxic and no mutagenesis, and the treatment characteristic is arranged.It can make food such as beverage, meat product, egg in foodstuffs industry tinting material also can be used for industries such as pharmacy, makeup.Because the cyanidin extract aqueous solution of the present invention is also at Na
+Or Cu
2+When ion existed, its color became yellow-green colour by light red; At Fe
3+And/or Zn
2+When existing, its color presents scarlet, and this moment, the aqueous solution absorbing wavelength of this extract was 560 and 570nm.These physico-chemical properties also are intuitively can survey, and differentiate to have the obvious color distinctive easily.The extraction solvent that is adopted by the extraction extracting method that extracts cyanidin extract in the halophytes floral leaf of the present invention obtains than being easier to, and its solvent also is easy recycling.And, in this halophytes floral leaf raw material of every 100g, can obtain the cyanidin extract of 1.0-4.0g, promptly the raw material yield is 1.0-4.2%.Therefore, extracting method running cost of the present invention is low, has reduced environmental pollution.Of the present invention is that halophytes floral leaf extracting solution is adopted AP-8 type polymeric adsorbent refinement treatment by the polymeric adsorbent method for refining that extracts cyanidin extract in the halophytes floral leaf, ethanolic soln wash-out with 60%-80%, utilized the soluble in water of anthocyanidin, be slightly soluble in ethanol character, anthocyanidin is eluted from polymeric adsorbent, collect this ethanol eluate, can obtain containing the higher elutriant dilute concentration cyanidin extract of anthocyanidin.This elutriant promptly obtains the cyanidin extract that the raw material extraction yield is 1.0-4.0% again through secondary concentration and spraying drying.
Embodiment
Embodiments of the invention have embodied this patent protection domain, but this patent protection domain does not limit to following examples.
Bright Suaeda heteroptera (suaeda heteroptera) the plant sample of example one: 25.0g adds 100ml water, and tissue homogenate filters, and puts in the closed glass bottle, obtains homogenate 105g.Get 70g, add 40ml acetone, the 20ml sherwood oil extracts.The upper strata is a green (pollution free) petro ether layer, and lower floor is red water/acetone layer, gets water layer after the layering, and underpressure distillation is removed moisture and got the 0.7g pigment, raw material yield=((0.7 * (105/70))/25.0) * 100%=4.2%.
Example two: get the halophytes floral leaf of handling the 100g Suaeda salsa (suaeda salsa) after the cleaning, put into refiner, add 250 gram clean waters, homogenate obtained halophytes floral leaf slurries in 15 minutes, with described halophytes floral leaf slurries, put into whizzer, through centrifugal treating 30 minutes, then supernatant liquor is carried out vacuum filtration, remove the remaining cellulosic in the described supernatant liquor, obtain halophytes floral leaf extracting solution 260ml;
Get this halophytes floral leaf extracting solution 70g again, add 40ml acetone, 20 sherwood oils extract.The upper strata is a green (pollution free) petro ether layer, and lower floor is red water/acetone layer, gets water layer after the layering, and concentrating under reduced pressure is spray-dried, obtains red pigments 0.94 gram, raw material yield=((0.94 * (260/70))/100) * 100%=3.5%.
Example three: the dry floral leaf of 100g salicornia europaeal (Salicornia europaea) adds 300 gram clean waters after crushed, and 50 ℃ were extracted 60 minutes, extracting solution through centrifugal, filter after, obtain halophytes floral leaf extracting solution.This halophytes floral leaf extracting solution adopts polymeric adsorbent refining, with anthocyanidin 60% ethanolic soln that is adsorbed in the resin, extracting, obtain ethanolic soln, described ethanolic soln is inserted rotatory evaporator, and vacuum concentration under 60 ℃ of temperature obtains this halophytes floral leaf extract 1.5 grams with the aqueous solution after concentrating through spray thing drying, this halophytes floral leaf extract is to have hygroscopic red-purple powder material, the yield of gained raw material=(1.5/100) * 100%=1.5%.
Claims (10)
1, a kind of cyanidin extract, it is characterized in that: described extract is by extracting in the halophytes floral leaf, it has the hygroscopic red-purple powdery substance that is, the anthocyanidin that contains 25-70% in this extract, this extract is water-soluble, be slightly soluble in ethanol and trichloromethane, be insoluble to benzene, toluene, ether, sherwood oil; This extract is 529nm at the visible region maximum absorption wavelength; The color that the aqueous solution of this extract presents, and presents by dark red → red → pale red → pale yellow → Huang → deep yellow variation by 3 to 13 variation with the pH value; The aqueous solution of this extract was placed under natural light after 72 hours, and its solution appearance color becomes light red by redness, and the absorbance value under the 529nm wavelength drops to 1.80 by 2.0; And UV-light does not have obvious influence to the aqueous solution of this extract.
2, according to the described cyanidin extract of claim 1, it is characterized in that: described this solution of extract is also at Na
+Or Cu
2+When ion existed, its color became yellow-green colour by light red; At Fe
3+And/or Zn
2+When existing, its color presents scarlet, and this moment, the aqueous solution absorbing wavelength of this extract was 560 and 570nm.
3, according to claim 1 or 2 described cyanidin extracts, it is characterized in that: described halophytes is: Suaeda heteroptera (suaeda heteroptera), salicornia europaeal (Salicornia europaea), Suaeda salsa (suaedasalsa).
4, according to the extracting method of claim 1 or 2 described cyanidin extracts, it is characterized in that: the step of this method is,
A), will handle clean quantitative halophytes floral leaf, put into refiner, add 1-3 clean water doubly, homogenized 5-15 minute, gained halophytes floral leaf slurries are put into whizzer,, get supernatant liquor and carry out vacuum filtration again through centrifugal treating 10-30 minute, remove the remaining cellulosic in this supernatant liquor, promptly obtain halophytes floral leaf extracting solution;
B), get above-mentioned halophytes floral leaf extracting solution, add the polar solvent of this extracting liquid volume 30-70% and the non-polar solvent of 20-40%, mix and stirred 10-30 minute, the normal temperature standing demix extracts, the upper strata is green oil reservoir, and lower floor is red water layer, gets this water layer through concentrating under reduced pressure, spray-dried again, obtain red-purple powdery substance one cyanidin extract.
5, according to the extracting method of the described cyanidin extract of claim 4, it is characterized in that: described polar solvent, it is an acetone, or propyl alcohol, or ethanol, or ethyl acetate, wherein one or more.
6, according to the extracting method of the described cyanidin extract of claim 4, it is characterized in that: described non-polar solvent, it is a sherwood oil, or ether, or methylene dichloride, or chloroform, or contains the N-alkanes hydro carbons of 5-8 carbon.
7, according to the another kind of extracting method of claim 1 or 2 described cyanidin extracts, it is characterized in that: the step of this method is,
A), will handle clean quantitative halophytes floral leaf drying and pulverize after, add 1-3 clean water doubly, under 30-50 ℃ of temperature, soak and extracted 30-60 minute, with soaked extracting solution through centrifugal, filter after, get filtrate and obtain halophytes floral leaf extracting solution;
B), above-mentioned halophytes floral leaf extracting solution is adopted AP-8 type polymeric adsorbent refinement treatment, with the ethanolic soln wash-out of 60%-80%, collect this ethanol eluate;
C), the gained ethanol eluate puts into rotatory evaporator again, evaporation concentration, again through vacuum concentration that this elutriant after concentrating is spray-dried again, promptly obtain having hygroscopic red-purple powder material-cyanidin extract.
8, according to the extracting method of the described cyanidin extract of claim 7, it is characterized in that: described AP-8 polymeric adsorbent, its dress post specification is: resin bottom face 1.5 * 25cm, resin load height 15cm.
9, according to the extracting method of the described cyanidin extract of claim 7, it is characterized in that: described vacuum concentration is under 60 ℃ of temperature, carries out under vacuum tightness-0.08Mpa.
10, according to the extracting method of the described cyanidin extract of claim 7, it is characterized in that: described spraying drying is 240 ℃ of air inlet temperatures, and 80 ℃ of air outlet temperatures carry out under the condition of air compressor machine pressure 0.4MPa.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2004100236487A CN1560048A (en) | 2004-03-02 | 2004-03-02 | Extractive of smalt and extracting process thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2004100236487A CN1560048A (en) | 2004-03-02 | 2004-03-02 | Extractive of smalt and extracting process thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1560048A true CN1560048A (en) | 2005-01-05 |
Family
ID=34441100
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2004100236487A Pending CN1560048A (en) | 2004-03-02 | 2004-03-02 | Extractive of smalt and extracting process thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1560048A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102653634A (en) * | 2011-03-02 | 2012-09-05 | 盐城工学院 | Ultrasound-assisted method for extracting betacyanin from suaeda salsa |
| CN105175381A (en) * | 2015-10-14 | 2015-12-23 | 盐城工学院 | Method of extracting and purifying anthocyanin from suaeda salsa |
| CN105361182A (en) * | 2015-10-14 | 2016-03-02 | 盐城工学院 | Composite stabilizer formulation containing suaeda salsa anthocyanin and application of composite stabilizer formulation |
| CN115677642A (en) * | 2022-07-20 | 2023-02-03 | 孙大利 | Method for extracting anthocyanin from suaeda salsa |
-
2004
- 2004-03-02 CN CNA2004100236487A patent/CN1560048A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102653634A (en) * | 2011-03-02 | 2012-09-05 | 盐城工学院 | Ultrasound-assisted method for extracting betacyanin from suaeda salsa |
| CN105175381A (en) * | 2015-10-14 | 2015-12-23 | 盐城工学院 | Method of extracting and purifying anthocyanin from suaeda salsa |
| CN105361182A (en) * | 2015-10-14 | 2016-03-02 | 盐城工学院 | Composite stabilizer formulation containing suaeda salsa anthocyanin and application of composite stabilizer formulation |
| CN115677642A (en) * | 2022-07-20 | 2023-02-03 | 孙大利 | Method for extracting anthocyanin from suaeda salsa |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102060833A (en) | Method for extracting anthocyanin from lycium ruthenicum fruit | |
| CN105524482A (en) | Hylocereus polyrhizus flesh and pericarp red pigment and extraction method thereof | |
| CN103788218B (en) | A kind of Rhizoma Dioscoreae esculentae effective ingredient decomposes extracting method | |
| CN102659743A (en) | Extraction rectification method of anthocyanin in mulberry | |
| CN101253987B (en) | Preparation of raspberry natural red coloring matter | |
| CN102604424A (en) | Method for extracting anthocyanins from purple sweet potato | |
| CN104041821A (en) | Lycium ruthenicum murr anthocyanin chewable tablet | |
| CN1212320C (en) | Resin process of extracting proanthocyanidin from graup seed | |
| CN102429275A (en) | Plant herbal beverage and preparation method thereof | |
| CN107652337B (en) | Method for extracting and preparing anthocyanin such as black rice, black beans, purple potatoes, roses, peonies and the like | |
| CN1304488C (en) | Technique for extracting edible natural red color element from psammophyte of nitraria | |
| CN101775233A (en) | Membrane separation integrated technology-based preparation method for producing deodorized red radish pigment | |
| CN102757663A (en) | Chestnut shell pigment grading method | |
| CN1560048A (en) | Extractive of smalt and extracting process thereof | |
| CN105901681A (en) | Preparation methods for extract liquid, fermentation extracellular liquid and fermentation intracellular liquid of dictyophora, and application thereof | |
| CN105175381A (en) | Method of extracting and purifying anthocyanin from suaeda salsa | |
| CN105410279A (en) | Composite tea composed of extracts of siraitia grosvenorii and radix isatidis and preparation method thereof | |
| CN102786507B (en) | Simple method for extracting food-grade anthocyanin in purple potato | |
| CN104761596A (en) | Method for preparing anthocyanin standard substance | |
| CN101311225A (en) | Process for abstracting kohlrabi pigment | |
| CN1075517C (en) | Method for extracting solomonscal polyose from Chinese drug solomonseal rhizome | |
| CN103146476A (en) | Green extraction technique capable of comprehensively and efficiently utilizing sophora flower bud | |
| KR101039217B1 (en) | Enhanced Health Functional Patterned Fabrics Printed with Tannin and Process for Preparation thereof | |
| CN102229753A (en) | Method for extracting purple corn pigment | |
| CN104256847B (en) | A kind of efficient defecation method of cider |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| AD01 | Patent right deemed abandoned | ||
| C20 | Patent right or utility model deemed to be abandoned or is abandoned |