CN105410279A - Composite tea composed of extracts of siraitia grosvenorii and radix isatidis and preparation method thereof - Google Patents

Composite tea composed of extracts of siraitia grosvenorii and radix isatidis and preparation method thereof Download PDF

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CN105410279A
CN105410279A CN201510973867.XA CN201510973867A CN105410279A CN 105410279 A CN105410279 A CN 105410279A CN 201510973867 A CN201510973867 A CN 201510973867A CN 105410279 A CN105410279 A CN 105410279A
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extract
momordica grosvenori
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weight
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程忠泉
杨丹
温宇
吴丽文
孙显屏
徐宝敏
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Echo Bio Tech Ltd Guilin
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Echo Bio Tech Ltd Guilin
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23FCOFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
    • A23F3/00Tea; Tea substitutes; Preparations thereof
    • A23F3/34Tea substitutes, e.g. matè; Extracts or infusions thereof

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Abstract

The invention discloses a composite tea composed of extracts of siraitia grosvenorii and radix isatidis and a preparation method thereof. The composite tea is composed of, by mass, 25% of the siraitia grosvenorii extract and 75% of the radix isatidis extract. The preparation method includes the steps of pre-treatment, preparation of a siraitia grosvenorii peel extract, preparation of a siraitia grosvenorii seed extract, preparation of the radix isatidis extract, and mixing and packaging. In the invention, the extracts are employed as the raw materials, wherein peels and seeds of the siraitia grosvenorii are separated and are extracted in different methods, so that dissolving-out speed of active components in the siraitia grosvenorii and radix isatidis is greatly increased and further utilization rate and absorption rate of the materials are increased. The preparation method develops the commercial values of the siraitia grosvenorii and radix isatidis.

Description

A kind of Momordica grosvenori isatis root extract compound tea and preparation method thereof
Technical field
The present invention relates to a kind of Momordica grosvenori isatis root extract compound tea and preparation method thereof, belong to tea technical field.
Background technology
Tea is not only the diuresis of a kind of tool cardiac stimulant, the health beverages of the effect such as arousing brain, raising spirit, especially indispensable bavin in people's life, rice, oil, salt, sauce, vinegar, the one in tea.Though tea has a long history, but develop by leaps and bounds along with soda, fruit drink and mineral water, the status of tea little by little reduces.However, along with development and the people's living standard raising of society, more and more people focuses on health, and tea is a kind of health beverages meeting modern.This be due to modern tea be utilize pure natural plant to be deducted a percentage by scientific technology after add to inside drink tea, the existing good health value of tea is had again and certain drinks effect.
Momordica grosvenori, belonging to cucurbitaceous plant, is the medicinal and edible plant of Chinese tradition, and has effect of clearing heat and moistening lung, laxation defaecation, promotion stomach.Fruits nutrition is worth very high, and containing abundant vitamin C and glucoside, fructose, glucose, protein, lipid etc., wherein, the nonsugar triterpenes components mogrosides of strong sweet taste is the main active ingredient of Momordica grosvenori.
Radix Isatidis, also known as great Lan root, is the dry root of Cruciferae woaded blue platymiscium woaded blue, bitter, and cold in nature, the thoughts of returning home, stomach warp, have heat-clearing, removing toxic substances, cool blood, the effects such as relieve sore throat, can treat the diseases such as influenza, warm disease heating, abscess of throat, the headache of high heat.Radix Isatidis effective component has alkaloid, organic acid, flavonoids, lignanoids, ester class, amino acids, ucleosides, coumarin kind compound etc., wherein organic acid has significantly antibacterial, anti-endotoxin effect, and then makes Radix Isatidis have good heat-clearing toxin-expelling functions.
Because Momordica grosvenori and Radix Isatidis have so many effect, people carry out many research to them, as Chinese patent CN104543259A discloses one " processing technology of " Luohan " fruit tea fine powder ", the method take Momordica grosvenori as raw material, be made through steps such as raw material preparation, extraction, enzymolysis, the enzyme that goes out, filtration, pulverizing, mixing, spraying dry, finishing and packings, it mainly solves the nutriment losing issue in caffein powder; CN102113589B discloses one " health protection tea and preparation method thereof ", this tea is made up of ginseng 12 ~ 16 parts, dried longan pulp 12 ~ 26 parts, matrimony vine 18 parts, Momordica grosvenori 4 parts, Jasmine 5 ~ 10 parts, honeysuckle 8 ~ 11 parts, Radix Isatidis 12 ~ 16 parts, lotus nut 4 parts, the sterculia seed 6 ~ 9 parts, 8 parts, Radix Glycyrrhizae, ginkgo leaf 18 ~ 22 parts, 15 ~ 30 parts, rock sugar, and there is raising body immunity, preventing cold, effect of beauty care; CN104068408A discloses one " the aloe Momordica grosvenori compound capsule that a kind of mogroside IV content is high ", this capsule be by primary colors aloe leaf freeze dried powder 5-10%, mogroside IV content be Fructus Monordicae extract 8-12%, the Honegsukle flower P.E 6-12% of 15%-75%, isatis root extract 4-8%, cornstarch 30-40%, maltodextrin 10-25%, microcrystalline cellulose 10-15% make, and have the effect of prevention and therapy third liver, and side effect is low; CN104644705A discloses one " a kind of preparation method of Radix Isatidis thin membrane coated tablet ", and the method is mainly extracted make Radix Isatidis coating tablet have good hydrophily, coagulability, invertibity, caking property and palatability by being added Luo Han Guo Paste In Foods and ultrasonic wave in coating solution.Although the research of Momordica grosvenori and Radix Isatidis has obtained certain achievement, the research that the compound tea active ingredient dissolution rate about their compositions crosses slow problem has remained very limited, and this limits the development of Momordica grosvenori Radix Isatidis compound tea to a certain extent.Therefore, it is very important for developing a kind of preparation method improving Momordica grosvenori Radix Isatidis compound tea active ingredient dissolution velocity.
Summary of the invention
The technical problem to be solved in the present invention is to overcome the deficiencies in the prior art, the invention provides a kind of Momordica grosvenori isatis root extract compound tea and preparation method thereof.
The present invention for the taked technical scheme that solves the problem is:
A kind of Momordica grosvenori isatis root extract compound tea, it is made up of Fructus Monordicae extract and isatis root extract, and described Fructus Monordicae extract and the mass percent of isatis root extract are 25%:75%; Described Fructus Monordicae extract is made up of Momordica grosvenori bark extract and Momordica grosvenori seed extract, and wherein Momordica grosvenori bark extract adopts water extraction to extract, and Momordica grosvenori seed extract adopts ultrasonic method to extract; Described isatis root extract adopts water logging to extract ethanol precipitation and extracts.
The preparation method of described Momordica grosvenori isatis root extract compound tea comprises following technique: pretreatment, the preparation of Momordica grosvenori bark extract, the preparation of Momordica grosvenori seed extract, isatis root extract preparation and hybrid packed five steps:
(1) pretreatment: first arhat pericarp is separated with seed, then by clean, the sterilization respectively of arhat pericarp, Momordica grosvenori seed and Radix Isatidis, be ground into powder, and cross 100 ~ 200 mesh sieves, for subsequent use;
(2) Momordica grosvenori bark extract preparation: by arhat fruit peel powder 100 weight portion of selecting step (1), decoct three times in 95 ~ 115 DEG C, add the distilled water of 6 times of weight for the first time, decoct 85 ~ 105 minutes, second time adds the distilled water of 3 times of weight, decoct 45 ~ 65 minutes, add the distilled water of 2 times of weight for the third time, decoct 25 ~ 35 minutes, filter, merging filtrate, then filtrate to be placed in rotating speed be on the centrifuge of 6000 ~ 8000 turns centrifugal 25 ~ 35 minutes, again filters, concentrated, spray drying treatment, obtains Momordica grosvenori bark extract powder, for subsequent use;
(3) Momordica grosvenori seed extract preparation: by Momordica grosvenori seed meal 100 weight portion of selecting step (1), be 50 ~ 70kHz in frequency, 30 ~ 60 DEG C of ultrasonic lower extractions three times, add the distilled water of 5 times of weight for the first time, ultrasonic 65 ~ 85 minutes, second time adds the distilled water of 3 times of weight, ultrasonic 45 ~ 65 minutes, add the distilled water of 1 times of weight for the third time, ultrasonic 25 ~ 45 minutes, filter, merging filtrate, on the centrifuge again filtrate being placed in 6000 ~ 8000 turns centrifugal 25 ~ 35 minutes, again filter, concentrated, spray drying treatment, obtain Momordica grosvenori seed extract powder, for subsequent use,
(4) isatis root extract preparation: by Radix Isatidis powder 100 weight portion of selecting step (1), decoct three times in 110 ~ 150 DEG C, add the distilled water of 8 times of weight for the first time, decoct 90 ~ 100 minutes, second time adds the distilled water of 6 times of weight, decoct 45 ~ 65 minutes, add the distilled water of 3 times of weight for the third time, decoct 10 ~ 20 minutes, filter, merging filtrate, on the centrifuge again filtrate being placed in 6000 ~ 8000 turns centrifugal 25 ~ 35 minutes, again filter, adding ethanol regulates determining alcohol to 30 ~ 60%, leave standstill, to be precipitated completely after, incline and supernatant, filter, isatis root extract powder is obtained after concentrated, for subsequent use,
(5) hybrid packed:
The Momordica grosvenori bark extract powder, Momordica grosvenori seed extract powder and the isatis root extract powder that first step (2), (3), (4) are obtained respectively through freeze drying, pulverized 500 ~ 600 mesh sieves; And then Momordica grosvenori bark extract powder and Momordica grosvenori seed extract powder are fully mixed, obtain Fructus Monordicae extract; Be finally that Fructus Monordicae extract and the isatis root extract of 25%:75% fully mixes by mass percent, Momordica grosvenori isatis root extract compound tea can be obtained.
The nonsugar triterpenes components mogrosides of the strong sweet taste in Fructus Monordicae extract, optimizes the taste of Radix Isatidis, improves the palatability of Momordica grosvenori isatis root extract compound tea.Simultaneously in Fructus Monordicae extract also containing multiple inorganic elements, as calcium, magnesium, selenium, and elemental selenium has good curative effect at coronary heart disease control, anti-ageing, anticancer aspect.Fructus Monordicae extract have eliminate the phlegm, the pharmacology such as antibechic, anti-inflammatory and antalgic is antibacterial and improve immune effect.The one-tenth such as alkaloid, organic acid, flavonoids, lignanoids, ester class, amino acids, ucleosides, coumarin kind compound are had in isatis root extract, wherein organic acid has significantly antibacterial, anti-endotoxin effect, and then makes Radix Isatidis have good heat-clearing toxin-expelling functions.Fructus Monordicae extract combines with isatis root extract, can overcome the defect of single extract, and respective effect can be made again more effectively to embody, thus makes effect of compound tea more effective comprehensively.
Compared with prior art, the invention has the beneficial effects as follows:
1, adopt extract as raw material, the nutrition of original compound tea can be retained, its active ingredient dissolution rate can be accelerated again, decrease the loss of active ingredient, improve utilization rate and the absorptivity of material, for the commercial value realizing Momordica grosvenori and Radix Isatidis has opened up approach;
2, be separated with seed by arhat pericarp, greatly can improve the recovery rate of Momordica grosvenori active ingredient, thus promote active ingredient dissolution rate, optimize the taste of Radix Isatidis bitter, improve the palatability of Momordica grosvenori isatis root extract compound tea, too increase absorptivity and effect of compound tea simultaneously;
3, adopt different extracting methods and often kind three times extract, effectively reduce the loss of compound tea active ingredient, thus increase the content of active ingredient, and then promote compound tea active ingredient dissolution rate, the extraction time of often kind of extracting method becomes staged simultaneously, improves compound tea active ingredient dissolution rate further;
4, ultrasonic process can be conducive at utmost protecting Organic Selenium, reduces the high-temperature oxydation degraded of Organic Selenium, improves effect of compound tea;
5, the preparation method of Momordica grosvenori isatis root extract compound tea provided by the present invention, its preparation formula is reasonable, technique is simple, with short production cycle, and equipment investment expense is low, greatly increases production efficiency, reduces production cost.
Detailed description of the invention
Embodiment 1
A preparation method for Momordica grosvenori isatis root extract compound tea, comprises the following steps:
(1) pretreatment: first arhat pericarp is separated with seed, then by clean, the sterilization respectively of arhat pericarp, Momordica grosvenori seed and Radix Isatidis, be ground into powder, and cross 150 mesh sieves, for subsequent use;
(2) Momordica grosvenori bark extract preparation: by arhat fruit peel powder 100 weight portion of selecting step (1), decoct three times in 105 DEG C, add the distilled water of 6 times of weight for the first time, decoct 95 minutes, second time adds the distilled water of 3 times of weight, decoct 55 minutes, add the distilled water of 2 times of weight for the third time, decoct 30 minutes, filter, merging filtrate, then filtrate to be placed in rotating speed be on the centrifuge of 7000 turns centrifugal 30 minutes, again filters, concentrated, spray drying treatment, obtains Momordica grosvenori bark extract powder, for subsequent use;
(3) Momordica grosvenori seed extract preparation: by Momordica grosvenori seed meal 100 weight portion of selecting step (1), be 60kHz, 45 DEG C of ultrasonic lower extractions three times in frequency, add the distilled water of 5 times of weight for the first time, ultrasonic 75 minutes, second time adds the distilled water of 3 times of weight, ultrasonic 55 minutes, add the distilled water of 1 times of weight for the third time, ultrasonic 35 minutes, filter, merging filtrate, then on centrifuge filtrate being placed in 7000 turns centrifugal 30 minutes, again filter, concentrated, spray drying treatment, obtains Momordica grosvenori seed extract powder, for subsequent use;
(4) isatis root extract preparation: by Radix Isatidis powder 100 weight portion of selecting step (1), decoct three times in 130 DEG C, add the distilled water of 8 times of weight for the first time, decoct 95 minutes, second time adds the distilled water of 6 times of weight, decoct 55 minutes, add the distilled water of 3 times of weight for the third time, decoct 15 minutes, filter, merging filtrate, on the centrifuge again filtrate being placed in 7000 turns centrifugal 30 minutes, again filter, adding ethanol regulates determining alcohol to 45%, leave standstill, to be precipitated completely after, incline and supernatant, filter, isatis root extract powder is obtained after concentrated, for subsequent use,
(5) hybrid packed:
The Momordica grosvenori bark extract powder, Momordica grosvenori seed extract powder and the isatis root extract powder that first step (2), (3), (4) are obtained respectively through freeze drying, pulverized 600 mesh sieves; And then Momordica grosvenori bark extract powder and Momordica grosvenori seed extract powder are fully mixed, obtain Fructus Monordicae extract; Be finally that Fructus Monordicae extract and the isatis root extract of 25%:75% fully mixes by mass percent, Momordica grosvenori isatis root extract compound tea can be obtained.
Carry out Se content detection by GB/t21729-2008 National Standard Method to obtained Momordica grosvenori isatis root extract compound tea, testing result is 272 μ g/kg.
Embodiment 2
A preparation method for Momordica grosvenori isatis root extract compound tea, comprises the following steps:
(1) pretreatment: first arhat pericarp is separated with seed, then by clean, the sterilization respectively of arhat pericarp, Momordica grosvenori seed and Radix Isatidis, be ground into powder, and cross 100 mesh sieves, for subsequent use;
(2) Momordica grosvenori bark extract preparation: by arhat fruit peel powder 100 weight portion of selecting step (1), decoct three times in 95 DEG C, add the distilled water of 6 times of weight for the first time, decoct 85 minutes, second time adds the distilled water of 3 times of weight, decoct 45 minutes, add the distilled water of 2 times of weight for the third time, decoct 25 minutes, filter, merging filtrate, then filtrate to be placed in rotating speed be on the centrifuge of 6000 turns centrifugal 25 minutes, again filters, concentrated, spray drying treatment, obtains Momordica grosvenori bark extract powder, for subsequent use;
(3) Momordica grosvenori seed extract preparation: by Momordica grosvenori seed meal 100 weight portion of selecting step (1), be 50kHz, 30 DEG C of ultrasonic lower extractions three times in frequency, add the distilled water of 5 times of weight for the first time, ultrasonic 65 minutes, second time adds the distilled water of 3 times of weight, ultrasonic 45 minutes, add the distilled water of 1 times of weight for the third time, ultrasonic 25 minutes, filter, merging filtrate, then on centrifuge filtrate being placed in 6000 turns centrifugal 25 minutes, again filter, concentrated, spray drying treatment, obtains Momordica grosvenori seed extract powder, for subsequent use;
(4) isatis root extract preparation: by Radix Isatidis powder 100 weight portion of selecting step (1), decoct three times in 110 DEG C, add the distilled water of 8 times of weight for the first time, decoct 90 minutes, second time adds the distilled water of 6 times of weight, decoct 45 minutes, add the distilled water of 3 times of weight for the third time, decoct 10 minutes, filter, merging filtrate, on the centrifuge again filtrate being placed in 6000 turns centrifugal 25 minutes, again filter, adding ethanol regulates determining alcohol to 30%, leave standstill, to be precipitated completely after, incline and supernatant, filter, isatis root extract powder is obtained after concentrated, for subsequent use,
(5) hybrid packed:
The Momordica grosvenori bark extract powder, Momordica grosvenori seed extract powder and the isatis root extract powder that first step (2), (3), (4) are obtained respectively through freeze drying, pulverized 500 mesh sieves; And then Momordica grosvenori bark extract powder and Momordica grosvenori seed extract powder are fully mixed, obtain Fructus Monordicae extract; Be finally that Fructus Monordicae extract and the isatis root extract of 25%:75% fully mixes by mass percent, Momordica grosvenori isatis root extract compound tea can be obtained.
Carry out Se content detection by GB/t21729-2008 National Standard Method to obtained Momordica grosvenori isatis root extract compound tea, testing result is 256 μ g/kg.
Embodiment 3
A preparation method for Momordica grosvenori isatis root extract compound tea, comprises the following steps:
(1) pretreatment: first arhat pericarp is separated with seed, then by clean, the sterilization respectively of arhat pericarp, Momordica grosvenori seed and Radix Isatidis, be ground into powder, and cross 200 mesh sieves, for subsequent use;
(2) Momordica grosvenori bark extract preparation: by arhat fruit peel powder 100 weight portion of selecting step (1), decoct three times in 115 DEG C, add the distilled water of 6 times of weight for the first time, decoct 105 minutes, second time adds the distilled water of 3 times of weight, decoct 65 minutes, add the distilled water of 2 times of weight for the third time, decoct 35 minutes, filter, merging filtrate, then filtrate to be placed in rotating speed be on the centrifuge of 8000 turns centrifugal 35 minutes, again filters, concentrated, spray drying treatment, obtains Momordica grosvenori bark extract powder, for subsequent use;
(3) Momordica grosvenori seed extract preparation: by Momordica grosvenori seed meal 100 weight portion of selecting step (1), be 70kHz, 60 DEG C of ultrasonic lower extractions three times in frequency, add the distilled water of 5 times of weight for the first time, ultrasonic 85 minutes, second time adds the distilled water of 3 times of weight, ultrasonic 65 minutes, add the distilled water of 1 times of weight for the third time, ultrasonic 45 minutes, filter, merging filtrate, then on centrifuge filtrate being placed in 8000 turns centrifugal 35 minutes, again filter, concentrated, spray drying treatment, obtains Momordica grosvenori seed extract powder, for subsequent use;
(4) isatis root extract preparation: by Radix Isatidis powder 100 weight portion of selecting step (1), decoct three times in 150 DEG C, add the distilled water of 8 times of weight for the first time, decoct 100 minutes, second time adds the distilled water of 6 times of weight, decoct 65 minutes, add the distilled water of 3 times of weight for the third time, decoct 20 minutes, filter, merging filtrate, on the centrifuge again filtrate being placed in 8000 turns centrifugal 35 minutes, again filter, adding ethanol regulates determining alcohol to 60%, leave standstill, to be precipitated completely after, incline and supernatant, filter, isatis root extract powder is obtained after concentrated, for subsequent use,
(5) hybrid packed:
The Momordica grosvenori bark extract powder, Momordica grosvenori seed extract powder and the isatis root extract powder that first step (2), (3), (4) are obtained respectively through freeze drying, pulverized 600 mesh sieves; And then Momordica grosvenori bark extract powder and Momordica grosvenori seed extract powder are fully mixed, obtain Fructus Monordicae extract; Be finally that Fructus Monordicae extract and the isatis root extract of 25%:75% fully mixes by mass percent, Momordica grosvenori isatis root extract compound tea can be obtained.
Carry out Se content detection by GB/t21729-2008 National Standard Method to obtained Momordica grosvenori isatis root extract compound tea, testing result is 266 μ g/kg.
Embodiment 4
A preparation method for Momordica grosvenori isatis root extract compound tea, comprises the following steps:
(1) pretreatment: first arhat pericarp is separated with seed, then by clean, the sterilization respectively of arhat pericarp, Momordica grosvenori seed and Radix Isatidis, be ground into powder, and cross 180 mesh sieves, for subsequent use;
(2) Momordica grosvenori bark extract preparation: by arhat fruit peel powder 100 weight portion of selecting step (1), decoct three times in 110 DEG C, add the distilled water of 6 times of weight for the first time, decoct 100 minutes, second time adds the distilled water of 3 times of weight, decoct 60 minutes, add the distilled water of 2 times of weight for the third time, decoct 30 minutes, filter, merging filtrate, then filtrate to be placed in rotating speed be on the centrifuge of 7500 turns centrifugal 32 minutes, again filters, concentrated, spray drying treatment, obtains Momordica grosvenori bark extract powder, for subsequent use;
(3) Momordica grosvenori seed extract preparation: by Momordica grosvenori seed meal 100 weight portion of selecting step (1), be 65kHz, 50 DEG C of ultrasonic lower extractions three times in frequency, add the distilled water of 5 times of weight for the first time, ultrasonic 80 minutes, second time adds the distilled water of 3 times of weight, ultrasonic 60 minutes, add the distilled water of 1 times of weight for the third time, ultrasonic 40 minutes, filter, merging filtrate, then on centrifuge filtrate being placed in 7500 turns centrifugal 30 minutes, again filter, concentrated, spray drying treatment, obtains Momordica grosvenori seed extract powder, for subsequent use;
(4) isatis root extract preparation: by Radix Isatidis powder 100 weight portion of selecting step (1), decoct three times in 140 DEG C, add the distilled water of 8 times of weight for the first time, decoct 92 minutes, second time adds the distilled water of 6 times of weight, decoct 60 minutes, add the distilled water of 3 times of weight for the third time, decoct 18 minutes, filter, merging filtrate, on the centrifuge again filtrate being placed in 7500 turns centrifugal 32 minutes, again filter, adding ethanol regulates determining alcohol to 50%, leave standstill, to be precipitated completely after, incline and supernatant, filter, isatis root extract powder is obtained after concentrated, for subsequent use,
(5) hybrid packed:
The Momordica grosvenori bark extract powder, Momordica grosvenori seed extract powder and the isatis root extract powder that first step (2), (3), (4) are obtained respectively through freeze drying, pulverized 500 mesh sieves; And then Momordica grosvenori bark extract powder and Momordica grosvenori seed extract powder are fully mixed, obtain Fructus Monordicae extract; Be finally that Fructus Monordicae extract and the isatis root extract of 25%:75% fully mixes by mass percent, Momordica grosvenori isatis root extract compound tea can be obtained.
Carry out Se content detection by GB/t21729-2008 National Standard Method to obtained Momordica grosvenori isatis root extract compound tea, testing result is 260 μ g/kg.
Embodiment 5: compound tea cough-relieving efficacy test
1, by body weight 18 ~ 22g and clinical symptoms are healthy mouse 120, be divided into embodiment 1 group, embodiment 2 groups, embodiment 3 groups, control group 1, control group 2 and blank group at random, often organize 20;
2, carry out gavage by 0.5ml/20g reagent to mouse, gavage, after 1 hour, adopts 30% ammoniacal liquor to draw the method for coughing and carries out cough-relieving test, namely in closed glass container, ammoniacal liquor is full of, mouse is put into glass container, records and add up mouse and to cough in 3 minutes number of times, the results are shown in Table 1.
Wherein, the reagent of each group gavage is: compound tea prepared by the compound tea of the embodiment 1 group of gavage embodiment of the present invention 1 preparation, embodiment 2 groups of gavage embodiment of the present invention 2, the compound tea of the embodiment 3 groups of gavage embodiment of the present invention 3 preparations, control group 1 gavage Fructus Monordicae extract, control group 2 gavage isatis root extract and blank group gavage physiological saline.
Table 1 mouse cough result of the test
Test group Cough number of times
Embodiment 1 group 26.48±7.38
Embodiment 2 groups 29.58±7.63
Embodiment 3 groups 28.45±8.14
Control group 1 37.87±9.62
Control group 2 39.36±9.87
Blank group 48.24±10.42
From table 1 result, compound tea of the present invention can reduce mouse cough number of times, has better antitussive action to the mouse cough that ammoniacal liquor causes.
Certainly, just the preferred embodiments of the disclosure is described in detail above, not limits practical range of the present invention with this, and all equivalence changes done according to principle of the present invention, structure and structure, all should be covered by protection scope of the present invention.

Claims (2)

1. a Momordica grosvenori isatis root extract compound tea, is characterized in that: it is made up of Fructus Monordicae extract and isatis root extract, and described Fructus Monordicae extract and the mass percent of isatis root extract are 25%:75%; Described Fructus Monordicae extract is made up of Momordica grosvenori bark extract and Momordica grosvenori seed extract, and wherein Momordica grosvenori bark extract adopts water extraction to extract, and Momordica grosvenori seed extract adopts ultrasonic method to extract; Described isatis root extract adopts water logging to extract ethanol precipitation and extracts;
The preparation method of described Momordica grosvenori isatis root extract compound tea, it comprises following technique: pretreatment, the preparation of Momordica grosvenori bark extract, the preparation of Momordica grosvenori seed extract, isatis root extract preparation and hybrid packed five steps:
(1) pretreatment: first arhat pericarp is separated with seed, then by clean, the sterilization respectively of arhat pericarp, Momordica grosvenori seed and Radix Isatidis, be ground into powder, and cross 100 ~ 200 mesh sieves, for subsequent use;
(2) Momordica grosvenori bark extract preparation: by arhat fruit peel powder 100 weight portion of selecting step (1), decoct three times in 95 ~ 115 DEG C, add the distilled water of 6 times of weight for the first time, decoct 85 ~ 105 minutes, second time adds the distilled water of 3 times of weight, decoct 45 ~ 65 minutes, add the distilled water of 2 times of weight for the third time, decoct 25 ~ 35 minutes, filter, merging filtrate, then filtrate to be placed in rotating speed be on the centrifuge of 6000 ~ 8000 turns centrifugal 25 ~ 35 minutes, again filters, concentrated, spray drying treatment, obtains Momordica grosvenori bark extract powder, for subsequent use;
(3) Momordica grosvenori seed extract preparation: by Momordica grosvenori seed meal 100 weight portion of selecting step (1), be 50 ~ 70kHz in frequency, 30 ~ 60 DEG C of ultrasonic lower extractions three times, add the distilled water of 5 times of weight for the first time, ultrasonic 65 ~ 85 minutes, second time adds the distilled water of 3 times of weight, ultrasonic 45 ~ 65 minutes, add the distilled water of 1 times of weight for the third time, ultrasonic 25 ~ 45 minutes, filter, merging filtrate, on the centrifuge again filtrate being placed in 6000 ~ 8000 turns centrifugal 25 ~ 35 minutes, again filter, concentrated, spray drying treatment, obtain Momordica grosvenori seed extract powder, for subsequent use,
(4) isatis root extract preparation: by Radix Isatidis powder 100 weight portion of selecting step (1), decoct three times in 110 ~ 150 DEG C, add the distilled water of 8 times of weight for the first time, decoct 90 ~ 100 minutes, second time adds the distilled water of 6 times of weight, decoct 45 ~ 65 minutes, add the distilled water of 3 times of weight for the third time, decoct 10 ~ 20 minutes, filter, merging filtrate, on the centrifuge again filtrate being placed in 6000 ~ 8000 turns centrifugal 25 ~ 35 minutes, again filter, adding ethanol regulates determining alcohol to 30 ~ 60%, leave standstill, to be precipitated completely after, incline and supernatant, filter, isatis root extract powder is obtained after concentrated, for subsequent use,
(5) hybrid packed:
The Momordica grosvenori bark extract powder, Momordica grosvenori seed extract powder and the isatis root extract powder that first step (2), (3), (4) are obtained respectively through freeze drying, pulverized 500 ~ 600 mesh sieves; And then Momordica grosvenori bark extract powder and Momordica grosvenori seed extract powder are fully mixed, obtain Fructus Monordicae extract; Be finally that Fructus Monordicae extract and the isatis root extract of 25%:75% fully mixes by mass percent, Momordica grosvenori isatis root extract compound tea can be obtained.
2. the preparation method of Momordica grosvenori isatis root extract compound tea according to claim 1, is characterized in that: described ultrasonic extraction is 60kHz in frequency, extracts at 45 DEG C.
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CN107410587A (en) * 2017-04-21 2017-12-01 长兴紫茗茶文化传播有限公司 A kind of preparation method of the sharp leaf instant tea of Momordica grosvenori dragon with clearing lung and eliminating phlegm effect
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CN111494450A (en) * 2020-03-19 2020-08-07 贺州学院 Preparation method of isatis root indigo-momordica grosvenori seed tea
CN111494450B (en) * 2020-03-19 2022-01-28 贺州学院 Preparation method of isatis root indigo-momordica grosvenori seed tea

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