CN1560005A - Process for extracting compound of hemp phenol from hemp seed oil - Google Patents
Process for extracting compound of hemp phenol from hemp seed oil Download PDFInfo
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- CN1560005A CN1560005A CNA2004100135999A CN200410013599A CN1560005A CN 1560005 A CN1560005 A CN 1560005A CN A2004100135999 A CNA2004100135999 A CN A2004100135999A CN 200410013599 A CN200410013599 A CN 200410013599A CN 1560005 A CN1560005 A CN 1560005A
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Abstract
The invention discloses a method of extracting phenolic compound hemp phenolic compound from hemp seed oil, taking a certain amount of hemp seed oil, adding in 4-6 times alcohol water solution with concentration 80-95%, making water bath and ultrasonic extraction for 15 minutes, centrifuging, and taking out supernatant to obtain pure edible oil at once. It uses alcohol water solution to extract and separate toxic component-hemp phenolic compound from hemp seed oil, the process is simple, low-cost, after extraction, none of the flavor, color and appearance of the oil change obviously, it does not destroy nutrition constituents in the oil and has no residual toxic components, the yield is high, the extraction is high and it is suitable for industrialized production.
Description
Technical field
The invention belongs to field of fine chemical, be specifically related to the extracting method of hemp phenoloid in a kind of hemp seed oil.
Background technology
Hemp, promptly hemp has another name called fiery fiber crops etc., moraceae plants, 1 year all one's life draft.Seed can extract oil, and also can be used as medicine.Contain a large amount of unsaturated fatty acidss and a certain amount of hemp phenoloid in hemp seed and the oil thereof, unsaturated fatty acids has very strong antioxygenation, health to the human body cardiovascular and cerebrovascular is highly profitable, but hemp phenoloid-cannabinol, tetrahydrocannabinol, the cannabidiol that contains in hemp seed and the oil has toxicity simultaneously, after removing the toxic component in the sesame oil, this sesame oil can be used as a kind of good edible oil and uses.The applicant once adopted silica gel column chromatography and alumina column chromatography method that hemp phenoloid in the sesame oil is carried out extraction separation, but the rate of recovery of sesame oil only is 58-62% behind the extraction separation, and yield is not high, and speed is slow, and the time is long, the cost height.
Summary of the invention
The object of the present invention is to provide the extracting method of hemp phenoloid in a kind of hemp seed oil, this method can thoroughly be removed the hemp phenoloid in the flaxseed oil, obtains colory hemp seed edible oil simultaneously.
The extracting method of hemp phenoloid is in the hemp seed oil of the present invention: get a certain amount of hemp seed oil, adding 4～6 times of amount concentration is 80～95% aqueous ethanolic solution, water-bath supersound extraction 15 minutes, centrifugal, take out supernatant liquor, remaining oil is used with quadrat method water-bath supersound extraction again 2～3 times, centrifugal, take out supernatant liquor.
The physico-chemical property of the hemp phenoloid that is contained in the hemp seed oil is, be soluble in benzene, chloroform, ether, hexanol, methyl alcohol organic solvent etc., wherein methyl alcohol is to the dissolving power minimum of hemp seed oil, next is an ethanol, though the extraction effect of methyl alcohol is best, but owing to the health of its toxicity to the operator constitutes a threat to, and easily residue in the oil, be unfavorable for producing.Dehydrated alcohol is bigger to the dissolving power of oil, reduces the yield of oil, also is difficult for centrifugation simultaneously.The present invention utilizes aqueous ethanolic solution extraction separation from hemp seed oil of 80%～95% to go out the hemp phenoloid, technology is simple, cost is low, considerable change does not all take place in smell, color and the outward appearance of extracting back oil, do not destroy the nutrition in the oil, the nontoxicity composition is residual, the yield height, the extraction efficiency height is fit to suitability for industrialized production.
Alcohol concn is big more, and extraction efficiency is high more, and centrifugal separating effect is good more, but its dissolved ability is strong more, the yield of easy more influence oil.
Take by weighing hemp seed oil 2ml, the concentration that adds 5 times of amounts of oil respectively is 95%, 90%, 85%, 80% ethanolic soln 10ml, water-bath supersound extraction 15 minutes, centrifugal, get its supernatant liquor solvent is volatilized, residue dissolves with the methyl alcohol gradation and is settled in the 10ml measuring bottle, shakes up, it is an amount of with behind the 0.45 μ m filtering with microporous membrane to get above-mentioned four kinds of solution, get subsequent filtrate 20 μ L and inject high performance liquid chromatograph and analyze, the results are shown in Table one, extract 4 numbers after, remaining oil is purified edible oil.
Table one
From peak area, after extracting three times, the cannabinol in the hemp seed oil, cannabidiol and tetrahydrocannabinol have been extracted totally substantially, the 4th time extract concentrations are increased to be twice, do not detect above-mentioned three kinds of materials, consider that extraction time can be for 3-4 time from cost and time and security standpoint.From peak area as can be seen, 95% extraction using alcohol rate is the highest, secondly is 90% ethanol, and the easy and oily emulsification of 85% and 80% ethanol is difficult for centrifugal settling, and extraction efficiency is relatively low.
Precision weighing extracts the quality of oil remaining after four times, calculated yield.The result is: with concentration is 95% extraction using alcohol, and the yield of oil is 76.98%, is 90% extraction using alcohol with concentration, the yield of oil is 92.7%, is 85% extraction using alcohol with concentration, and oily yield is 91.88%, with concentration is 80% extraction using alcohol, and the yield of oil is 90.76%.
Take all factors into consideration, it is that 90% aqueous ethanolic solution water-bath supersound extraction is preferred plan three times that the present invention adopts the concentration of 5 times of hemp seed oil consumptions.
Claims (4)
1, the extracting method of hemp phenoloid in a kind of hemp seed oil, it is characterized in that: get a certain amount of hemp seed oil, adding 4～6 times of amount concentration is 80～95% aqueous ethanolic solution, water-bath supersound extraction 15 minutes, centrifugal, take out supernatant liquor, remaining oil is used with quadrat method water-bath supersound extraction again 2-3 time, centrifugal, take out supernatant liquor.
2, the extracting method of hemp phenoloid in the hemp seed oil according to claim 1 is characterized in that: the consumption of aqueous ethanolic solution is 5 times of hemp seed oil.
3, the extracting method of hemp phenoloid in the hemp seed oil according to claim 1 and 2, it is characterized in that: described aqueous ethanolic solution optimum concn is 90%.
4, the extracting method of hemp phenoloid in the described hemp seed oil according to claim 1 is characterized in that: the best number of times of described water-bath supersound extraction is 3 times.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2004100135999A CN1560005A (en) | 2004-02-27 | 2004-02-27 | Process for extracting compound of hemp phenol from hemp seed oil |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2004100135999A CN1560005A (en) | 2004-02-27 | 2004-02-27 | Process for extracting compound of hemp phenol from hemp seed oil |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1560005A true CN1560005A (en) | 2005-01-05 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| CNA2004100135999A Pending CN1560005A (en) | 2004-02-27 | 2004-02-27 | Process for extracting compound of hemp phenol from hemp seed oil |
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| CN (1) | CN1560005A (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101333221B (en) * | 2008-07-24 | 2011-01-19 | 武汉大学 | Stabilization method for annular L-proline anhydride configuration |
| CN104031736A (en) * | 2014-07-01 | 2014-09-10 | 辽宁晟麦实业股份有限公司 | Preparation method for hemp seed oil with low tetrahydrocannabinol content |
| CN110683938A (en) * | 2019-08-27 | 2020-01-14 | 桂林莱茵生物科技股份有限公司 | Preparation method of cannabidiol |
| US11040932B2 (en) | 2018-10-10 | 2021-06-22 | Treehouse Biotech, Inc. | Synthesis of cannabigerol |
| US11084770B2 (en) | 2016-12-07 | 2021-08-10 | Treehouse Biotech, Inc. | Cannabis extracts |
| US11202771B2 (en) | 2018-01-31 | 2021-12-21 | Treehouse Biotech, Inc. | Hemp powder |
| CN114258960A (en) * | 2021-12-21 | 2022-04-01 | 吉林农业大学 | Detoxified hemp seed cheese |
-
2004
- 2004-02-27 CN CNA2004100135999A patent/CN1560005A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101333221B (en) * | 2008-07-24 | 2011-01-19 | 武汉大学 | Stabilization method for annular L-proline anhydride configuration |
| CN104031736A (en) * | 2014-07-01 | 2014-09-10 | 辽宁晟麦实业股份有限公司 | Preparation method for hemp seed oil with low tetrahydrocannabinol content |
| CN104031736B (en) * | 2014-07-01 | 2015-11-18 | 辽宁晟麦实业股份有限公司 | A kind of preparation method of fructus cannabis oil of low tetrahydrocannabinol content |
| US11084770B2 (en) | 2016-12-07 | 2021-08-10 | Treehouse Biotech, Inc. | Cannabis extracts |
| US11202771B2 (en) | 2018-01-31 | 2021-12-21 | Treehouse Biotech, Inc. | Hemp powder |
| US11040932B2 (en) | 2018-10-10 | 2021-06-22 | Treehouse Biotech, Inc. | Synthesis of cannabigerol |
| CN110683938A (en) * | 2019-08-27 | 2020-01-14 | 桂林莱茵生物科技股份有限公司 | Preparation method of cannabidiol |
| CN110683938B (en) * | 2019-08-27 | 2022-11-15 | 桂林莱茵生物科技股份有限公司 | Preparation method of cannabidiol |
| CN114258960A (en) * | 2021-12-21 | 2022-04-01 | 吉林农业大学 | Detoxified hemp seed cheese |
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