CN1472207A - Extracting method of polyphenol from lotus root - Google Patents
Extracting method of polyphenol from lotus root Download PDFInfo
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- CN1472207A CN1472207A CNA031282156A CN03128215A CN1472207A CN 1472207 A CN1472207 A CN 1472207A CN A031282156 A CNA031282156 A CN A031282156A CN 03128215 A CN03128215 A CN 03128215A CN 1472207 A CN1472207 A CN 1472207A
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- polyphenol
- lotus rhizome
- lotus
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- ethyl acetate
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Abstract
A process for extracting polyphenol from lotus root includes such steps as pulverizing lotus root, immersing in alcohol solution, heating while stirring, filtering, thermal concentrating, extracting with ethyl acetate, concentrating, drying to obtain coarse product, dissolving in acetone solution, separating and purifying. Its advantages are high quality of product, low cost and no pollution.
Description
Technical field:
The present invention relates to biological technical field, more specifically relate to the extracting method of polyphenol in a kind of lotus rhizome.
Background technology:
As far back as the beginning of the sixties in last century, Japanese scientist has just verified tealeaves and has contained and have in a large number than the active material of strong anti-oxidation---tea-polyphenol.To the eighties in last century, the technology of preparing of tea-polyphenol has bigger development, has entered the practical stage of suitability for industrialized production.The tealeaves antioxidant commodity of selling in the international market have nearly ten kinds of three kinds of formulations (water-based, oiliness, powdery) at present.Since the nineties in last century, China has also developed extraction process of several tea-polyphenol and products thereof in succession, and has carried out suitability for industrialized production.
Lotus rhizome is the important cash crop of China, and is closely during the last ten years, very fast in the cultivated area growth of China.Exist a large amount of polyphenol substances in the lotus rhizome.The polyphenol of lotus rhizome is based on catechin, and is similar with tea-polyphenol, mainly comprises anthocyanidin and xantheine two big classes, and wherein xantheine comprises flavones and derivative, riboflavin etc. again.A series of important pharmacological functions such as that tea-polyphenol has is anticancer, anti-ageing, radioprotective, removing human free radical, lowering blood glucose blood fat have broad application prospects in fields such as grease, food, medicine, daily use chemicals.The extraction of lotus rhizome polyphenol substance (being called for short the lotus rhizome polyphenol), to help comprehensive utilization to lotus rhizome, promote the development of lotus rhizome industry, this not only can make contributions for ensureing people ' s health, but also can increase peasant's income, create huge economic benefit, the guiding industrial structure develops to high speed.
With regard to the extracting method of tea-polyphenol in the tealeaves, can be divided into four classes (being organic solvent extractionprocess, alkali solution precipitate method, ion-exchange-resin process, supercritical extraction) according to its principle, they have following characteristics respectively:
Organic solvent extractionprocess.Its principle is to utilize tea-polyphenol to be soluble in or water soluble, ethanol, ether, propyl carbinol equal solvent and it is extracted from tealeaves, or with organic solvents such as ethyl acetate it is extracted from aqueous extract.This method is a kind of maturation and the method widely used on producing, and the shortcoming of this method is to need with a large amount of organic solvent (comprising noxious solvents such as chloroform), and the energy that solvent recuperation and tea juice concentrate consumption is bigger.Particularly use poisonous organic solvents such as chloroform in this production technique, chloroform cost height, volatile, harmful, technology is also complicated, makes to produce and operation safety etc. inadequately.
The alkali solution precipitate method.Its principle is to utilize tea-polyphenol in certain precipitation medium, by co-precipitation enrichment tea-polyphenol, then through changeing the molten organic solvent extraction of using again.This method has reduced the consumption of organic solvent, and the production cycle shortens, and energy consumption is lower, but owing to used a large amount of heavy metallic salts to make precipitation agent, has influenced quality product, makes aftertreatment technology loaded down with trivial details, the equipment complexity.Although recent research has been developed nontoxic Ca cheap and easy to get
2+, Mg
2+, Al
2+, Zn
2+Deng normal salt is precipitation agent, but owing to tea-polyphenol in alkaline medium very easily oxidation make a large amount of tea-polyphenol oxidation wastages, thereby the utilization ratio of raw material (tealeaves) is reduced.
Ion-exchange-resin process.The technological operation of this method extraction tea-polyphenol is simple, and energy consumption is lower, but because the resin of selecting has higher selectivity and bigger equilibrium adsorption capacity to tea-polyphenol, realize that large-scale industrial production is still waiting further exploration.
Supercritical extraction.Supercutical fluid is a kind of high pressure, the dense fluids that material is presented when being in the above state of its stagnation point.Supercritical extraction is to utilize supercutical fluid to carry out the method for extracting and separating as solvent.This method rate of extraction is fast, and the extraction Functionality, quality and appealing design more effectively keeps volatile effective ingredient than the traditional extraction method, keeps " natural " true qualities, has broad application prospects.But this explained hereafter needs relatively large production unit, and this Ang of Cheng is expensive, and working condition is had relatively high expectations.
Summary of the invention:
The object of the present invention is to provide the extracting method of polyphenol in a kind of lotus rhizome, technology is easy, easy to operate, safety, and good product quality, low production cost, pollution-free.
To achieve these goals, the present invention by the following technical solutions.
The method that the present invention proposes has the following steps:
1, raw material pulverizing.Use general pulverizer that raw materials for production (lotus leaf, lotus stem, rhizoma nelumbinis, lotus root whip etc.) are pulverized.
2, lixiviate.Raw material after pulverizing is added ethanolic soln, and transferring PH is 3～3.5, heating, and temperature is controlled at 68-72 ℃, stirring and leaching; Time is 1-3 hour, adopts general press filtration or uses Plate Filtration.
3, concentrated and purified.Transferring filtrate PH is 10.5～11, heating, and temperature is controlled at 68-72 ℃, concentrates, and eliminates to ethanol evaporation; Again concentrated solution PH is adjusted to 3～3.5, makes chlorophyll change water-fast pheophorbide into and remove.
4, extraction.With this concentrated solution of ethyl acetate extraction.
5, concentrate drying.Acetic acid ethyl acetate extract is concentrated, drying obtains the thick product of lotus rhizome polyphenol.
6, refining.Thick product is dissolved in 40% acetone soln, adopts column chromatography for separation to purify, obtain purified lotus rhizome polyphenol.
The present invention compared with prior art has the following advantages and effect:
One: be rich in the phenolic substance tea-polyphenol in the tealeaves, as a kind of antioxidant from natural food, tea-polyphenol is listed in national standard by China.Tea-polyphenol is the complex body of polyphenolic compound, have step-down efficiently, reducing blood-fat, prevent and treat effects such as cardiovascular disorder, antisepsis and anti-inflammation, also have a series of important pharmacological functions such as anticancer, anti-ageing, radioprotective, removing human free radical, have broad application prospects in fields such as grease, food, medicine, daily use chemicals.Lotus rhizome is the Nymphaeceae nelumbium, and lotus rhizome is nutritious, and whole body is precious, and purposes is extremely wide.Not only have edibleness but also have pharmaceutical use and nourishing function.The pharmaceutical use of lotus rhizome is high, and each several part all can be used as medicine.The Compendium of Material Medica LI Shi-Zhen claims that lotus rhizome is " a clever root ", can prevent and treat intestinal cancer, cervical cancer, nasopharyngeal carcinoma, and tumour is dwindled.In addition, lotus rhizome can also be improved looks that fitness, U.S. flesh anti-spot, wrinkle resistant fine flour, the crow of nourishing blood send out, inducing resuscitation makes eye bright, enriching yin and nourishing kidney, hard marrow, defatting beauty, be quenched the thirst and calm the nerves, arresting bleeding and miscarriage prevention.Exist a large amount of polyphenol substances in the lotus rhizome, the lotus rhizome polyphenol is not so far also by real utilization.Extracting method for polyphenol in the lotus rhizome yet there are no report.Based on present more sophisticated and (the tealeaves tea-polyphenol extracts) organic solvent extractionprocess of widespread use on producing, various Physiology and biochemistry characteristics at lotus rhizome byproduct (lotus leaf, lotus stem, rhizoma nelumbinis, lotus root whip etc.), develop the novel technology for extracting of polyphenol in the cover lotus rhizome, filled up the blank in this field.
Its two: do not use organic solvents such as chloroform in this technology, do not have " three wastes " and pollute, simultaneously, the efficient of extracting the lotus rhizome polyphenol is also very high.The less investment instant effect is particularly suited for medium-sized and small enterprises and practices midwifery.
Description of drawings:
Fig. 1 is the extracting method block diagram of polyphenol in a kind of lotus rhizome.
Embodiment:
According to Fig. 1 the present invention is described in further detail:
According to Fig. 1 as can be known, lotus leaf is pulverized 1 and is obtained lotus leaf wood 2.Get lotus leaf end 20g, add the ethanolic soln of 160ml 70%, transferring PH is 3,70 ℃ of stirred in water bath lixiviates (time is respectively 1h, 2h, 3h), three filtrates is merged, and filters, and discards filter residue 3, keeps filtrate 4; Transferring filtrate PH is 11, concentrates in 70 ℃ of water-baths, eliminate to ethanol evaporation, concentrated solution 5; Again concentrated solution PH is adjusted to 3, makes chlorophyll be transformed into water-fast de-magging folic acid and disgorging 6, keep water layer 7.Water layer discards water layer 8 with duple ethyl acetate extraction secondary, keeps ethyl acetate layer 9; Steam and remove ethyl acetate, vacuum-drying gets the khaki solid 10 of 2.2g, extraction yield 11%, purity 70%.This crude product is dissolved in 40% acetone soln, carries out column chromatography (being filled with adsorbent A A) and separate purification, the gained purified product is a buff powder 11, content＞90%, the rate of recovery 80%.
Claims (1)
1, the extracting method of polyphenol in a kind of lotus rhizome comprises the following steps:
A, raw material pulverizing are pulverized lotus leaf with pulverizer;
B, lixiviate add ethanolic soln with the raw material after pulverizing, and transferring PH is 3～3.5, heating, and temperature is controlled at 68-72 ℃, stirring and leaching, the time is 1-3 hour, uses Plate Filtration;
C, concentrated and purified, transferring filtrate PH is 10.5～11, heating, temperature is controlled at 68-72 ℃, concentrates, and eliminates to ethanol evaporation, again concentrated solution PH is adjusted to 3～3.5, makes chlorophyll change water-fast pheophorbide into and removes;
D, extraction are with this concentrated solution of ethyl acetate extraction;
E, concentrate drying, acetic acid ethyl acetate extract is concentrated, drying obtains the thick product of lotus rhizome polyphenol;
F, refining is dissolved in thick product in 40% acetone soln, adopts column chromatography for separation to purify, and obtains purified lotus rhizome polyphenol.
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| CNA031282156A CN1472207A (en) | 2003-06-26 | 2003-06-26 | Extracting method of polyphenol from lotus root |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100425605C (en) * | 2005-09-01 | 2008-10-15 | 桂林莱茵生物科技股份有限公司 | Method for extracting persimmon polyphenol from persimmon |
| CN101007064B (en) * | 2006-09-09 | 2010-05-12 | 福州大学 | Isolation and purification method of lotus leaf extract by macroporous resin and its application thereof |
| CN101824021B (en) * | 2009-10-14 | 2013-01-09 | 南开大学 | Method for extracting catechin small molecules in lotus root and application thereof |
| CN104938646A (en) * | 2015-05-29 | 2015-09-30 | 武汉轻工大学 | Lotus root polyphenol extraction method, lotus root polyphenol oil-resistant oxidant preparation method, lotus root polyphenols, lotus root polyphenol oil-resistant oxidant, application of lotus root polyphenols, and application of lotus root polyphenol oil-resistant oxidant |
| CN107349239A (en) * | 2017-06-23 | 2017-11-17 | 百色学院 | A kind of extracting method of mango leaf polyphenol |
| CN108441372A (en) * | 2018-03-26 | 2018-08-24 | 安徽工程大学 | A kind of preparation method of lotus leaf polyphenol yellow rice wine |
| CN111150758A (en) * | 2018-11-07 | 2020-05-15 | 江苏省农业科学院 | Method for continuously preparing polyphenol, polysaccharide and dietary fiber from lotus rhizome nodes |
-
2003
- 2003-06-26 CN CNA031282156A patent/CN1472207A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100425605C (en) * | 2005-09-01 | 2008-10-15 | 桂林莱茵生物科技股份有限公司 | Method for extracting persimmon polyphenol from persimmon |
| CN101007064B (en) * | 2006-09-09 | 2010-05-12 | 福州大学 | Isolation and purification method of lotus leaf extract by macroporous resin and its application thereof |
| CN101824021B (en) * | 2009-10-14 | 2013-01-09 | 南开大学 | Method for extracting catechin small molecules in lotus root and application thereof |
| CN104938646A (en) * | 2015-05-29 | 2015-09-30 | 武汉轻工大学 | Lotus root polyphenol extraction method, lotus root polyphenol oil-resistant oxidant preparation method, lotus root polyphenols, lotus root polyphenol oil-resistant oxidant, application of lotus root polyphenols, and application of lotus root polyphenol oil-resistant oxidant |
| CN107349239A (en) * | 2017-06-23 | 2017-11-17 | 百色学院 | A kind of extracting method of mango leaf polyphenol |
| CN108441372A (en) * | 2018-03-26 | 2018-08-24 | 安徽工程大学 | A kind of preparation method of lotus leaf polyphenol yellow rice wine |
| CN111150758A (en) * | 2018-11-07 | 2020-05-15 | 江苏省农业科学院 | Method for continuously preparing polyphenol, polysaccharide and dietary fiber from lotus rhizome nodes |
| CN111150758B (en) * | 2018-11-07 | 2022-03-18 | 江苏省农业科学院 | Method for continuously preparing polyphenol, polysaccharide and dietary fiber from lotus rhizome nodes |
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