CN1557832A - Peocess for extracting ursolic acid from flower of loquat - Google Patents
Peocess for extracting ursolic acid from flower of loquat Download PDFInfo
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- CN1557832A CN1557832A CNA2004100228813A CN200410022881A CN1557832A CN 1557832 A CN1557832 A CN 1557832A CN A2004100228813 A CNA2004100228813 A CN A2004100228813A CN 200410022881 A CN200410022881 A CN 200410022881A CN 1557832 A CN1557832 A CN 1557832A
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- ethanol
- loquat leaf
- filtering
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- impurities
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Abstract
The present invention is the process of extracting ursolic acid from loquat leaf. The technological process includes water soaking, washing and partially dissolving loquat leaf to obtain partial water soluble matter; filtering, air drying, crushing and sieving; adding ethanol for Soxlet's extraction, regulating pH, concentration and temperature, eliminating impurity and filtering; vacuum concentrating the extract to obtain coarse crystal while recovering ethanol; filtering, ethanol dissolving precipitate, adding decolorizing agent and reflux decolorizing; crystallizing the filter liquid for 3-6 times to eliminate impurity, and filtering to obtain ursolic acid crystal with purity over 90 %. The process is simple, low in cost, low in pollution, high in yield and has other advantages. The product may be used in the development and application of various medicines.
Description
Technical field
The invention belongs to tcm field---the manufacture craft that Chinese herbal medicine effective ingredients extracts, particularly relating to Rosaceae Chinese medicine Loquat Leaf is the preparation method that raw material extracts ursonic acid.
Background technology
At present, extracting, separate ursonic acid abroad with Chinese Academy of Sciences institute of materia medica from Loquat Leaf adopts post to separate the method for wash-out.This method treatment capacity is little, cost height, only suitable laboratory study.About from Loquat Leaf, extracting the single active ingredient ursonic acid, still there is not relevant report at home and abroad with industrialized preparing process.
Summary of the invention
The purpose of this invention is to provide a kind of suitability for industrialized production that is suitable for, cost is low, yield is high, purity is high, solvent consumption is little, pollution is little, extract the preparation method of single active ingredient ursonic acid from Loquat Leaf.
The present invention realizes by following technical process:
With Loquat Leaf be soaked in water, behind washing, removal of impurities and the dissolving portion water dissolubility thing, filter is done; To filter dry material again through air seasoning, behind the crushing screening, add 80-95% ethanol, its weight be Loquat Leaf 2-6 doubly, extracted 6-10 hour through Soxhlet, extracting solution is adjusted PH7-12, concentration 70-90% and temperature 4-30 ℃, removal of impurities, filtration; Filtrate through vacuum concentration, reclaim ethanol to 20-60% volume, temperature 4-30 ℃, coarse crystallization, more after filtration, taking precipitate with dissolve with ethanol, add the discoloring agent reflux decolour; Filtrate through temperature 4-30 ℃, 3-6 the crystallization removal of impurities of ethanol of 80-95%, filter and obtain the ursonic acid crystallization that purity is 90-99%, drying promptly obtains product of the present invention again.
Product provided by the invention have pure natural, low toxicity, purity height, method simple, extract yield height, advantage that the prepared using degree is high.The present invention effectively utilizes southern area Loquat Leaf resource, make domestic utilization to Loquat Leaf from the cough-relieving application extension to antitumor, anti-hepatitis, exploitation, the application of calmness, anti-inflammatory, antibiotic, anti-diabetic, antiulcer agent, lowering blood glucose, medicine such as anti-oxidant; Promote the Chinese materia medica industry to develop, for industrial enterprise creates considerable economic benefit to high-quality, modernized, mass-producing direction; Improve the availability of plant simultaneously, increase the product high added value, be increasing peasant income, extra earning, make the poverty-stricken area peasant programme.
Description of drawings
Accompanying drawing is a process flow sheet of the present invention.
Specific embodiments
Get 10 kilograms of dried Loquat Leaves, soak removal of impurities and dissolving portion water soluble substance, take out filter and do through washing; After air seasoning, be ground into meal and cross the mesh sieve again, add 40 kilograms of ropes of 95% ethanol and carry 7-8 hour, extracting solution is through transferring concentration 80%, PH8, filtering and impurity removing; Filtrate is through vacuum concentration, recovery ethanol to 50% volume, 20 ℃ of coarse crystallization of room temperature; Again after filtration, taking precipitate with dissolve with ethanol, add the discoloring agent reflux decolour; Filtrate is through 20 ℃ of temperature, 4 crystallization removal of impurities of ethanol of 95%, and filtering and obtaining purity is that ursonic acid crystallization 82 90% or more restrains, and promptly obtains product of the present invention through vacuum drying again.
Claims (1)
1. preparation method who from Loquat Leaf, extracts ursonic acid, its technical process is:
(1) Loquat Leaf is soaked in water, behind washing, removal of impurities and the dissolving portion water dissolubility thing, filter is done;
(2) will filter dry material through air seasoning, behind the crushing screening, add 80-95% ethanol, its weight be Loquat Leaf 2-6 doubly, extracted 6-10 hour through Soxhlet, extracting solution is adjusted PH7-12, concentration 70-90% and temperature 4-30 ℃, removal of impurities, filtration;
(3) filtrate through vacuum concentration, reclaim ethanol to 20-60% volume, temperature 4-30 ℃, coarse crystallization, more after filtration, taking precipitate with dissolve with ethanol, add the discoloring agent reflux decolour;
(4) filtrate through temperature 4-30 ℃, 3-6 the crystallization removal of impurities of ethanol of 80-95%, filter and obtain the ursonic acid crystallization that purity is 90-99%, drying promptly obtains product of the present invention again.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2004100228813A CN1557832A (en) | 2004-02-06 | 2004-02-06 | Peocess for extracting ursolic acid from flower of loquat |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2004100228813A CN1557832A (en) | 2004-02-06 | 2004-02-06 | Peocess for extracting ursolic acid from flower of loquat |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1557832A true CN1557832A (en) | 2004-12-29 |
Family
ID=34352108
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2004100228813A Pending CN1557832A (en) | 2004-02-06 | 2004-02-06 | Peocess for extracting ursolic acid from flower of loquat |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1557832A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1980569A1 (en) * | 2007-04-04 | 2008-10-15 | Kenko Corporation | Process for preparing high purity corosolic acid and high purity ursolic acid |
| CN1931258B (en) * | 2006-09-21 | 2010-10-13 | 福州大学 | Preparation process and use of loquat seed extract |
| CN101220076B (en) * | 2008-01-10 | 2011-05-11 | 刘富来 | Method for extracting and separating prunol from Mesona Procumbens Hemsl |
-
2004
- 2004-02-06 CN CNA2004100228813A patent/CN1557832A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1931258B (en) * | 2006-09-21 | 2010-10-13 | 福州大学 | Preparation process and use of loquat seed extract |
| EP1980569A1 (en) * | 2007-04-04 | 2008-10-15 | Kenko Corporation | Process for preparing high purity corosolic acid and high purity ursolic acid |
| CN101220076B (en) * | 2008-01-10 | 2011-05-11 | 刘富来 | Method for extracting and separating prunol from Mesona Procumbens Hemsl |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |