CN101921343A - Method for extracting polysaccharide from fresh hedgehog hydnum - Google Patents
Method for extracting polysaccharide from fresh hedgehog hydnum Download PDFInfo
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- CN101921343A CN101921343A CN2010102329725A CN201010232972A CN101921343A CN 101921343 A CN101921343 A CN 101921343A CN 2010102329725 A CN2010102329725 A CN 2010102329725A CN 201010232972 A CN201010232972 A CN 201010232972A CN 101921343 A CN101921343 A CN 101921343A
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Abstract
The invention relates to a method for extracting polysaccharide from fresh hedgehog hydnum, which comprises the following steps of: leaching the fresh hedgehog hydnum in water at the temperature of between 55 and 65 DEG C for 2 hours, and filtering to obtain filter liquor; regulating a pH value of the filter liquor to between 4.0 and 4.5; performing chromatography on a HD-3 resin column for decoloring and deproteinizing, and performing ultrafiltration grading by using ceramic ultrafiltration membranes of which the molecular weight cutoff is 50 KD and 10 KD; and concentrating intermediate trapped fluid and spray-drying to prepare the polysaccharide, wherein the total recovery is 0.96 percent, and the purity is between 71 and 83 percent. The method has the advantages of high purity of the obtained hedgehog hydnum polysaccharide, simple and feasible process, no need of a drying process or an organic solvent, recycled resin, energy conservation, pollution reduction, and particular suitability for continuous industrial production.
Description
Technical field:
The present invention relates to utilize fresh Hericium erinaceus to extract the method for polysaccharide.
Background technology:
Hericium erinaceus (Bull. Ex Fr.) Pers. (Hericium erinaceus) is a kind of famous and precious edible and medicinal fungi, has sharp the five internal organs, aid digestion, nourishing and anticancer function.Its main active ingredient polysaccharide be main chain by β-1,3 glycosidic link, side chain is the dextran mixture that β-1,6 glycosidic link connects.Studies show that in a large number, effects such as that Hericium erinaceus polysaccharide has is antitumor, strengthen humoral immune function, hypoglycemic, reducing blood-fat, anticoagulation, anti-mutation, and diseases such as digestive system and ulcer, antral gastritis, chronic gastritis are all had good efficacy.Hericium erinaceum polysaccharide has great value medical health care.
Existing extracting method is many with the drying back of Hericium erinaceus (Bull. Ex Fr.) Pers. water, diluted acid, diluted alkaline lixiviate, and the method for organic solvent deposit is produced.This kind method extraction efficiency is low, expend a large amount of solvents, be difficult to carry out suitability for industrialized production, and gained polysaccharide goods purity is low, not only contains impurity such as a large amount of pigments, albumen, and inevitable dissolvent residual is arranged, and has limited the application of hericium erinaceum polysaccharide.The industrialization continuous production of research hericium erinaceum polysaccharide, the high purity polysaccharide that preparation has wide range of applications are the keys of China's mushroom industry upgrading.
Summary of the invention:
The present invention is to be raw material with the fresh Hericium erinaceus, the technology of producing the high purity polysaccharide of continuous high-efficient.
Technical process of the present invention:
(1) gets and cross colloidal mill 2～3 times after the new fresh Hericium erinaceus precrushing, add the water of 5～7 times of quality,, also can carry out the secondary lixiviate, filter merging filtrate in 55 ℃～65 ℃ following lixiviates 2 hours.
(2) regulating filtrate pH is 4.0～4.5, and filled media is the HD-3 resin in the last chromatography column, post, and chromatography decolouring deproteinated is collected elutriant under 4～5BV/h flow velocity.
(3) regulating elutriant pH is 6.5～7.5, under 0.1～0.25Mpa pressure, is that the ceramic membrane of 50KD and 10KD carries out ultrafiltration, spraying drying after trapped fluid concentrates in the middle of collecting with ultra-filtration membrane.
The present invention adopts resin decolorization deproteinated after the fresh Hericium erinaceus flooding, carries out the ultrafiltration classification again and concentrates, and spray-dired method is produced polysaccharide, and total recovery reaches 0.96%, and purity is 71%～83%.The technology simple possible does not need drying technics and organic solvent, and conserve energy reduces and pollutes, and is fit to very much suitability for industrialized production.
Embodiment:
Described a kind of concrete embodiment of method that utilizes fresh Hericium erinaceus to extract polysaccharide is as follows:
(1) select intact no septic fresh Hericium erinaceus for use,, add the water of 1.5 times of quality through crusher in crushing, again through milling treatment of colloid 2 times, the Hericium erinaceus (Bull. Ex Fr.) Pers. slurry.
(2) water of 6 times of quality of adding in the gained Hericium erinaceus (Bull. Ex Fr.) Pers. slurry, the back 60 ℃ of water-bath lixiviate 2h that stir filter and also collect filtrate.
(3) regulating filtrate pH value with dilute hydrochloric acid is 4.5, adopts the HD-3 resin column, and chromatography under the 5BV/h flow velocity is collected the 6BV elutriant.
(4) regulating elutriant pH with rare NaOH is 7.0, and adopting molecular weight cut-off earlier is the ultrafiltration of 50KD ultra-filtration membrane, collects filtrate, is the ultra-filtration membrane ultrafiltration of 10KD again with molecular weight cut-off, collects trapped fluid, and ultrafiltration pressure is 0.2Mpa.
(5) trapped fluid is concentrated into 1/3 volume, spraying drying gets hericium erinaceum polysaccharide, and recording hericium erinaceum polysaccharide purity is 76%.
Claims (2)
1. method of utilizing fresh Hericium erinaceus to extract polysaccharide, described method comprises:
(1) gets and cross colloidal mill 2～3 times after the new fresh Hericium erinaceus precrushing, add the water of 5～7 times of quality,, also can carry out the secondary lixiviate, filter merging filtrate in 55 ℃～65 ℃ following lixiviates 2 hours.
(2) regulating filtrate pH is 4.0～4.5, and filled media is the HD-3 resin in the last chromatography column, post, and chromatography decolouring deproteinated is collected 6～7BV elutriant under 4～5BV/h flow velocity.
(3) regulating elutriant pH is 6.5～7.5, is that the ceramic super-filtering film of 50KD and 10KD carries out ultrafiltration with molecular weight cut-off, and pressure is 0.1～0.25Mpa, trapped fluid in the middle of collecting, and spraying drying after concentrating obtains hericium erinaceum polysaccharide.
2. the method for claim 1 is characterized in that:
(1) determines best extracting technology
Utilize after the fresh Hericium erinaceus (Bull. Ex Fr.) Pers. fragmentation milling treatment of colloid 2～3 times, add the water of 5～7 times of quality, the control extraction temperature between 55 ℃～65 ℃, lixiviate 2 hours, after the filtration the polysaccharide vat liquor.
(2) determine optimum resin decolouring deproteinated technology
Adopt polysaccharide vat liquor in (1), utilize HD-3 resin column chromatography decolouring deproteinated, top condition is: regulating polysaccharide vat liquor pH value is 4.0～4.5, and the control flow velocity is 4～5BV/h, collects the elutriant of 6～7BV.
(3) ultrafiltration technology
Adopt (2) middle elutriant to carry out ultrafiltration, optimised process is: regulating elutriant pH value is 6.5～7.5, and adopting molecular weight cut-off is the ceramic super-filtering film of 50KD and 10KD, 0.1 ultrafiltration under the～0.25Mpa pressure, trapped fluid in the middle of collecting concentrates the back spraying drying, obtains hericium erinaceum polysaccharide.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010102329725A CN101921343A (en) | 2010-07-22 | 2010-07-22 | Method for extracting polysaccharide from fresh hedgehog hydnum |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2010102329725A CN101921343A (en) | 2010-07-22 | 2010-07-22 | Method for extracting polysaccharide from fresh hedgehog hydnum |
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| CN101921343A true CN101921343A (en) | 2010-12-22 |
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| CN2010102329725A Pending CN101921343A (en) | 2010-07-22 | 2010-07-22 | Method for extracting polysaccharide from fresh hedgehog hydnum |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102382199A (en) * | 2011-09-02 | 2012-03-21 | 广东太阳神集团有限公司 | High yield energy saving preparation method of Hericium erinaceus polysaccharide |
| CN102643359A (en) * | 2012-04-10 | 2012-08-22 | 上海市农业科学院 | Hericium erinaceus polysaccharide and preparation method thereof |
| CN105273100A (en) * | 2015-11-03 | 2016-01-27 | 广西南宁胜祺安科技开发有限公司 | Method for extracting hericium erinaceus polysaccharide from hericium erinaceus |
| CN107686527A (en) * | 2017-09-29 | 2018-02-13 | 华南理工大学 | A kind of extracting method of Hericium erinaceus antiallergic activity polysaccharide |
| CN114292888A (en) * | 2021-12-30 | 2022-04-08 | 赣州禾绿康健生物技术有限公司 | Preparation process of hericium erinaceus extract capable of improving immunity |
-
2010
- 2010-07-22 CN CN2010102329725A patent/CN101921343A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102382199A (en) * | 2011-09-02 | 2012-03-21 | 广东太阳神集团有限公司 | High yield energy saving preparation method of Hericium erinaceus polysaccharide |
| CN102382199B (en) * | 2011-09-02 | 2012-12-12 | 广东太阳神集团有限公司 | High yield energy saving preparation method of Hericium erinaceus polysaccharide |
| CN102643359A (en) * | 2012-04-10 | 2012-08-22 | 上海市农业科学院 | Hericium erinaceus polysaccharide and preparation method thereof |
| CN102643359B (en) * | 2012-04-10 | 2014-09-03 | 上海市农业科学院 | Hericium erinaceus polysaccharide and preparation method thereof |
| CN105273100A (en) * | 2015-11-03 | 2016-01-27 | 广西南宁胜祺安科技开发有限公司 | Method for extracting hericium erinaceus polysaccharide from hericium erinaceus |
| CN107686527A (en) * | 2017-09-29 | 2018-02-13 | 华南理工大学 | A kind of extracting method of Hericium erinaceus antiallergic activity polysaccharide |
| CN114292888A (en) * | 2021-12-30 | 2022-04-08 | 赣州禾绿康健生物技术有限公司 | Preparation process of hericium erinaceus extract capable of improving immunity |
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Application publication date: 20101222 |
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| WD01 | Invention patent application deemed withdrawn after publication |